Preparation method, product and application of Grass rose extract

Abstract

The invention provides a preparation method, a product and application of a Grass rose extract, and belongs to the technical field of cosmetic raw materials. The method comprises the following steps: mixing Grass rose with a solvent, and carrying out flash extraction to obtain a Grass rose extract; wherein the solvent is a butanediol aqueous solution. The content of astragalus smicus glycoside, ellagic acid and isoquercitrin in the Grass rose extract prepared by adopting the preparation method is higher, the proportion of astragalus smicus glycoside, ellagic acid and isoquercitrin is more beneficial to reducing the content of melanin in melanocytes and the activity of tyrosinase, and a basis is provided for application of the Grass rose extract in whitening cosmetics.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetic raw materials and relates to a preparation method, product and application of Grasse rose extract. Background Art

[0002] Grasse rose, also known as Rosa Centifolia L., is a plant of the genus Rosa in the family Rosaceae. The nodding flowers are born in late June or July, solitary or rarely in clusters, without bracts; often double; fragrant; from the bend of the petals to a transparent pink, very double, wine glass-shaped, and become looser when completely blown away, revealing tightly packed petals. The pedicels are slender, curved, and densely covered with glandular hairs; the sepals are ovate, with inconspicuous leaf-like tips, spread out, longer than the buds, and covered by the pedicels and buds, and like the pedicels and buds, they have sticky aromatic glands. Grasse rose is an internationally fragrant oil rose and an important source plant for extracting cosmetic raw materials.

[0003] In his paper "Study on Chemical Components and Quality Standards of Roses" (Master's thesis of Shandong University of Traditional Chinese Medicine, online publication date 2014.5.29), Ma Changhao conducted a preliminary study on the chemical components of the dried buds of the perennial shrub rose (Rosa rugosa Thunb.) of the Rosaceae family. Through spectral and chromatographic identification, six compounds were finally identified, namely gallic acid, protocatechuic acid, kaempferol, herbicide-8-methyl ether, isoquercitrin, and astragaloside. Using gallic acid and hyperoside as reference substances, an HPLC fingerprint of roses was established, providing a reference for rose identification and quality control. The ribosomal rDNA ITS sequences of roses of different cultivars were determined, providing a molecular biological basis for rose variety identification and germplasm resource optimization.

[0004] Chinese invention patent CN119032155A provides a method for preparing spice extracts and / or absolute oils, which method uses a method of contacting a specific, fresh, withered or dried solid natural material with a first solvent system comprising at least one "green" alkane solvent containing at least 8 carbon atoms. Another subject of the technology is a spice extract and spice absolute oil obtained by the preparation method, a composition comprising the extract and / or the absolute oil, and the use of the "green" alkane solvent for extracting the extract and / or the absolute oil without a chemical smell of the solvent smell type. The disclosure does not study the content of the active ingredient in the extract obtained by the extraction method, the ratio and the relationship between it and the whitening effect.

[0005] In summary, the prior art has not yet clarified the optimal ratio of various active ingredients in Grasse rose that have whitening effects, and has not yet provided a preparation process for a Grasse rose extract with a better ratio of ingredients for improving the whitening effect. Summary of the invention

[0006] In view of this, in view of the problems of the above-mentioned prior art, the purpose of the present invention is to provide a preparation method, product and application of Grasse rose extract. Taking the melanin content and tyrosinase activity of B16-F10 cells as indicators, the main monomers of rose, astragaloside, ellagic acid and isoquercetin, were screened in multiple ratios, and the ratio combination with the best whitening effect was successfully determined. On this basis, the Grasse rose extraction process was further screened and optimized. Under the condition of maintaining the optimal monomer ratio, by changing the preparation conditions in the extraction process, the monomer content in the Grasse rose extract was effectively improved, and a Grasse rose extract cosmetic raw material with significant whitening effect was provided, providing a basis for its application in cosmetics.

[0007] To achieve the above-mentioned object of the invention, on the one hand, the present invention provides a method for preparing a Grasse rose extract, comprising the following steps:

[0008] Grasse rose is mixed with solvent and flash extracted to obtain Grasse rose extract;

[0009] Wherein, the solvent is a butanediol aqueous solution.

[0010] Preferably, the Grasse rose is dried Grasse rose; and the butanediol is 1,3-butanediol.

[0011] Preferably, the butanediol aqueous solution is a butanediol aqueous solution with a weight concentration of 20%-40%.

[0012] More preferably, the butanediol aqueous solution is a butanediol aqueous solution with a weight concentration of 30%.

[0013] Preferably, the solid-liquid ratio of the Grasse rose to the solvent is 1:60-80, in g / mL.

[0014] More preferably, the solid-liquid ratio of the Grasse rose to the solvent is 1:70, expressed in g / mL.

[0015] Preferably, the time of the flash extraction is 20-40s, and the voltage of the flash extraction is 80-100V.

[0016] More preferably, the time of the flash extraction is 30s, and the voltage of the flash extraction is 80V.

[0017] Preferably, the Grasse rose is crushed before being mixed with the solvent.

[0018] Preferably, the flash extraction is followed by filtration and drying.

[0019] More preferably, the filtration is filter plate filtration.

[0020] As an example of the present invention, the pore size of the filter plate is 0.45 μm.

[0021] More preferably, the drying is freeze-drying.

[0022] On the other hand, the present invention provides a Grasse rose extract prepared by the above preparation method.

[0023] Preferably, the Grasse rose extract contains ellagic acid, isoquercetin and astragaloside.

[0024] More preferably, in the Grasse rose extract, the concentration of ellagic acid is 50-95 mg / g, the concentration of isoquercetin is 21-48 mg / g, and the concentration of astragaloside is 23-143 mg / g.

[0025] More preferably, the concentration ratio of astragaloside, ellagic acid and isoquercetin is 2.5-3.5::1.5-2.5:0.8-1.2, more preferably 2.9-3.1:1.9-2.1:0.9-1.1, and most preferably 3:2:1.

[0026] In another aspect, the present invention provides use of the Grasse rose extract obtained by the above preparation method in cosmetics.

[0027] In the last aspect, the present invention provides a cosmetic comprising the Grasse rose extract prepared by the above preparation method.

[0028] Compared with the prior art, the present invention has the following beneficial effects:

[0029] The present invention provides a method for preparing Grasse rose extract by flash extraction. The present invention uses B16-F10 cell melanin content and tyrosinase activity as indicators, screens various ratios of main components of Grasse rose, namely astragaloside, ellagic acid and isoquercetin, and successfully determines the ratio combination with the best whitening effect. Based on the screened optimal astragaloside, ellagic acid and isoquercetin ratio, the flash extraction technology is applied to the extraction of Grasse rose, and the process conditions of the flash extraction are optimized, so as to achieve an effective increase in the monomer content in the Grasse rose extract. A Grasse rose extract cosmetic raw material with significant whitening effect is provided, which provides a basis for its application in cosmetics. DETAILED DESCRIPTION

[0030] Terms and Claims of the Present Invention:

[0031] 1. The articles “a”, “an” and “the” include plural referents unless expressly limited to one or more referents otherwise.

[0032] 2. Numerical range: Unless otherwise expressly stated, all ranges or ratios disclosed herein will be understood to include any and all sub-ranges or sub-ratios contained therein. For example, a range or ratio of 1 to 30 stated should be considered to be included between a minimum value of 1 and a maximum value of 30, and any sub-range or sub-ratio, integer, decimal, or sub-range or sub-ratio consisting of integers or decimals including the end points.

[0033] The following non-limiting examples can enable those skilled in the art to more fully understand the present invention, but do not limit the present invention in any way. The following content is merely an exemplary description of the scope of the present invention, and those skilled in the art can make various changes and modifications to the present invention based on the disclosed content, which should also fall within the scope of the present invention.

[0034] The present invention is further described below by way of specific examples. The various chemical reagents used in the examples of the present invention are obtained through conventional commercial channels unless otherwise specified. Unless otherwise specified, the contents described below are all weight contents. Unless otherwise specified, it is understood that the process is carried out at room temperature.

[0035] In the following examples and comparative examples, the sources of some reagents are shown in Table 1:

[0036] Table 1

[0037] name Manufacturer / Source Item No. / Specification 1,3-Butanediol Shanghai Aladdin Biochemical Technology Co., Ltd. 1kg Astragaloside Shanghai Yuanye Biotechnology Co., Ltd. 20mg Ellagic acid Shanghai Yuanye Biotechnology Co., Ltd. 20mg Isoquercetin Shanghai Yuanye Biotechnology Co., Ltd. 20mg

[0038] In the following examples and comparative examples, some of the equipment information used is shown in Table 2:

[0039] Table 2

[0040]

[0041]

[0042] In the present invention, high performance liquid chromatography was used to detect the contents of astragaloside and ellagic acid, i.e., isoquercetin, in the Grasse rose extract in each embodiment / comparative example. The detection method is as follows:

[0043] (1) Chromatographic conditions and system suitability test: Agilent 1260 Infinity HPLC system was used to perform quantitative analysis of the components of the samples.

[0044] Instrument model: Agilent 1260; chromatographic column: ZORBAX SB-C18 (4.6 mm×250 mm, 5 μm); injection volume: 10 μL; detection wavelength: 254 nm; mobile phase: A: 0.3% formic acid aqueous solution B: acetonitrile; flow rate: 1.0 mL / min.

[0045] The elution program is shown in Table 3 below.

[0046] Table 3

[0047] Time (min) Mobile phase A (%) Mobile phase B (%) 0 95 5 5 95 5 10 90 10 15 85 15 25 85 15 35 80 20 45 70 30 55 60 40

[0048] (2) Preparation of reference solution: Accurately weigh three reference substances, namely ellagic acid, isoquercetin and astragaloside, respectively, and add methanol to prepare three solutions of 0.5 mg / ml. Then, take 300 μl of each of the three 0.5 mg / ml solutions, mix them evenly, and filter them through a 0.22 μm organic microporous filter. Take the filtrate to obtain the standard solution.

[0049] (3) Preparation of test solution: Take an appropriate amount of sample, accurately weigh it, and prepare a 1 mg / ml test solution with methanol. Pass it through a 0.22 μm organic microporous filter and take the filtrate.

[0050] (4) Determination method: Accurately pipette 10 μL of the reference solution and the test solution respectively, inject into the liquid chromatograph, and determine.

[0051] Experimental Example 1

[0052] Evaluation of the effects of different proportions of astragaloside, ellagic acid and isoquercetin mixture on melanin content and tyrosinase activity in B16-F10 cells.

[0053] Astragaloside, ellagic acid and isoquercetin are all active ingredients contained in Grasse rose. In order to explore what ratio of the above three ingredients has a better whitening effect, the following experiment was conducted.

[0054] Group 1: Accurately weigh astragaloside and dissolve it in DMSO liquid to prepare the mother solution, and use fresh culture medium to prepare liquids with concentrations of 10μg / mL, 5μg / mL, and 1μg / mL.

[0055] Group 2: Accurately weigh ellagic acid and dissolve it in DMSO liquid to prepare a stock solution, and use fresh culture medium to prepare liquids with concentrations of 10 μg / mL, 5 μg / mL, and 1 μg / mL.

[0056] Group 3: Accurately weigh isoquercetin and dissolve it in DMSO liquid to prepare the stock solution, and use fresh culture medium to prepare liquids with concentrations of 10 μg / mL, 5 μg / mL, and 1 μg / mL.

[0057] Group 4: The stock solutions of astragaloside, ellagic acid and isoquercetin were mixed in a ratio of 1:1:1 and prepared into liquids with concentrations of 10 μg / mL, 5 μg / mL and 1 μg / mL using fresh culture medium.

[0058] Group 5: The stock solutions of astragaloside, ellagic acid and isoquercetin were mixed in a ratio of 2:1:1 and prepared into liquids with concentrations of 10 μg / mL, 5 μg / mL and 1 μg / mL using fresh culture medium.

[0059] Group 6: The stock solutions of astragaloside, ellagic acid and isoquercetin were mixed in a ratio of 3:1:1 and prepared into liquids with concentrations of 10 μg / mL, 5 μg / mL and 1 μg / mL using fresh culture medium.

[0060] Group 7: The stock solutions of astragaloside, ellagic acid and isoquercetin were mixed in a ratio of 1:2:1 and prepared into liquids with concentrations of 10 μg / mL, 5 μg / mL and 1 μg / mL using fresh culture medium.

[0061] Group 8: The stock solutions of astragaloside, ellagic acid and isoquercetin were mixed in a ratio of 1:3:1 and prepared into liquids with concentrations of 10 μg / mL, 5 μg / mL and 1 μg / mL using fresh culture medium.

[0062] Group 9: The stock solutions of astragaloside, ellagic acid and isoquercetin were mixed in a ratio of 2:2:1 and prepared into liquids with concentrations of 10 μg / mL, 5 μg / mL and 1 μg / mL using fresh culture medium.

[0063] Group 10: The stock solutions of astragaloside, ellagic acid and isoquercetin were mixed in a ratio of 3:2:1 and prepared into liquids with concentrations of 10 μg / mL, 5 μg / mL and 1 μg / mL using fresh culture medium.

[0064] Group 11: The stock solutions of astragaloside, ellagic acid and isoquercetin were mixed in a ratio of 2:3:1 and prepared into liquids with concentrations of 10 μg / mL, 5 μg / mL and 1 μg / mL using fresh culture medium.

[0065] The experimental methods for the effects of the above 11 groups of solutions on the melanin inhibition rate and tyrosinase inhibition rate of B16-F10 cells are as follows.

[0066] 1. NaOH method to detect melanin inhibition rate of B16-F10 cells

[0067] (1)Packing

[0068] Collect B16-F10 cell solution and prepare 1×10 6 The cells / well were inoculated in a 6-well plate (sterile PBS solution was added to the remaining wells) and incubated in an incubator with 5% CO2 and 37°C.

[0069] (2) Sample loading

[0070] When 80%-90% of the B16-F10 cells adhered to the wall, the culture medium was discarded, and then washed twice with PBS buffer, and samples of corresponding concentrations were prepared with the culture medium (for those insoluble in the culture medium, the mother solution was prepared with DMSO), and sterilized by membrane. If the cells secreted melanin normally, the samples were directly loaded. The blank group was the model group. If the melanin secretion was less, α-MSH was added to stimulate the cells to secrete melanin. The group containing only α-MSH was the model group, and the group without α-MSH was the blank group. Each group had 2 replicate wells.

[0071] (3) Cell lysis

[0072] Place in the incubator and culture for 24 hours, aspirate the liquid in the well, wash with PBS, add the sample again after washing twice, and culture for 12 hours. Aspirate the liquid in the well. Add PBS, slowly adhere to the wall, wash twice, pour out the PBS, and use a 200μL pipette to absorb the remaining PBS. Add 400μL of 1mmol / LPMSF cell lysis solution to each well, lyse on ice for five minutes, collect the cell suspension and put it in a centrifuge tube. Centrifuge at 4℃, 12000r / min for 10min.

[0073] (4) Melanin determination

[0074] Transfer the supernatant to a new centrifuge tube and measure the total cell protein content. Melanin precipitates at the bottom of the centrifuge tube. Add 300 μL of 1M NaOH lysis solution (containing 10% DMSO) to the precipitate, vortex thoroughly, and place in a metal bath at 85°C for 20 minutes to completely dissolve the melanin precipitate. Take it out and vortex thoroughly, place 100 μL per well in a 96-well plate, set up 3 replicates, and measure the OD value at a wavelength of 405 nm.

[0075] (5) BCA method for determination of cell protein concentration

[0076] Prepare BCA solution according to the instructions of the BCA kit, add 25 μL of total cell protein solution and water to a 96-well plate, then add 200 μL of BCA solution, and make three duplicate wells for each tube. Place on a shaker at 37°C for 30 minutes, and measure the OD value at 562nm. (Use the BCA kit and operate according to the instructions of the kit) Calculate the total protein concentration based on the standard curve.

[0077] Melanin inhibition rate = 1-(OD value of experimental group / protein concentration) / (OD value of model group / protein concentration)

[0078] The experimental results of melanin inhibition rate of each group are shown in Table 4 below (mean ± standard deviation).

[0079] Table 4

[0080]

[0081]

[0082] It can be seen that in group 10 (the ratio of astragaloside, ellagic acid, and isoquercetin is 3:2:1), the melanin inhibition rate at different concentrations (10μg / mL, 5μg / mL, 1μg / mL) is the highest among all groups at the same concentration. That is, when the ratio of astragaloside, ellagic acid, and isoquercetin is 3:2:1, it has the best melanin inhibition effect.

[0083] 2. Determination of tyrosinase activity by L-DOPA oxidation method

[0084] Add 120 μg of total protein supernatant to a 1.5 mL centrifuge tube, add PBS buffer to the volume to 400 μL, vortex and mix, add to 96-well plates in sequence, add 100 μL of 0.1% L-DOPA, set 3 replicate wells for the same group, place in a 37°C oven and incubate in the dark for 1 hour. Measure the OD value at 475 nm with an enzyme reader.

[0085] Tyrosinase inhibition rate = 1-(experimental group / model group)

[0086] The experimental results of tyrosinase inhibition in each group are shown in Table 5 below (mean ± standard deviation).

[0087] Table 5

[0088]

[0089] It can be seen that in group 10 (the ratio of astragaloside, ellagic acid, and isoquercetin is 3:2:1), the tyrosinase inhibition rate at different concentrations (10μg / mL, 5μg / mL, 1μg / mL) is the highest among all groups at the same concentration. That is, when the ratio of astragaloside, ellagic acid, and isoquercetin is 3:2:1, it has the best tyrosinase inhibition effect.

[0090] In summary, when the ratio of astragalus glycosides, ellagic acid, and isoquercetin is 3:2:1, the melanin inhibition rate and tyrosinase inhibition rate of B16-F10 cells are the highest at 10, 5, and 1 μg / mL, and the whitening effect is the best.

[0091] Example 1

[0092] A method for preparing a Grasse rose extract.

[0093] Weigh 5 g of naturally air-dried Grasse rose flowers and crush them. Add 350 mL of 30% 1,3-butanediol aqueous solution at a solid-liquid ratio of 1:70, perform flash extraction for 30 s (voltage 90 V), filter the extract through a 0.45 μm filter plate, take half of it and freeze-dry it to obtain Grasse rose extract 1.

[0094] Comparative Example 1

[0095] Preparation of Grasse rose extract by microwave reflux extraction.

[0096] Weigh 5 g of naturally air-dried Grasse rose flowers and crush them. Add 350 mL of 30% 1,3-butanediol aqueous solution at a solid-liquid ratio of 1:70, and perform microwave reflux extraction for 150 min. After cooling to room temperature, the extract is filtered through a 0.45 μm filter plate and rotary evaporated to less than 87.5 mL to allow the ethanol to fully evaporate. Add 1,3-butanediol aqueous solution to 350 mL, take half of it and freeze-dry it to obtain Grasse rose extract D1.

[0097] Comparative Example 2

[0098] Preparation of Grasse rose extract by microwave reflux extraction.

[0099] Compared with Comparative Example 1, the difference is that 350 mL of 30% 1,3-butanediol aqueous solution is replaced by 350 mL of deionized water, and the rest is the same. The obtained extract is recorded as Grasse extract D2.

[0100] Comparative Example 3

[0101] Preparation of Grasse rose extract by ultrasound-assisted extraction.

[0102] Weigh 5 g of naturally air-dried Grasse rose flowers and crush them. Add 350 mL of 75% ethanol solution at a solid-liquid ratio of 1:70, perform ultrasonic-assisted extraction for 50 min, cool to room temperature, filter the extract through a 0.45 μm filter plate, and freeze-dry half of it to obtain Grasse rose extract D3.

[0103] The contents of astragaloside, ellagic acid and isoquercetin in the Grasse rose extracts obtained in Example 1 and Comparative Examples 1 to 3 were detected by HPLC. The results are shown in Table 6 below (mean ± standard deviation).

[0104] Table 6

[0105] Ellagic acid (mg / g) Isoquercetin(mg / g) Astragaloside(mg / g) Grasse Rose Extract 1 69.88±0.11 34.19±0.62 102.61±0.74 Grasse Rose Extract D1 92.10±0.39 44.48±0.15 90.48±0.11 Grasse Rose Extract D2 17.60±0.56 17.19±0.09 52.78±0.45 Grasse Rose Extract D3 29.5±0.19 39.99±0.55 99.03±0.15

[0106] Grasse rose extract 1, Grasse rose extract D1, Grasse rose extract D2, Grasse rose extract D3 were respectively prepared into mixed solutions with weight concentrations of 2.5%, 1.25% and 0.63% using fresh culture medium. The melanin inhibition rate and tyrosinase inhibition rate of each group of mixed solutions on B16-F10 cells were detected by the method provided in Experimental Example 1. The experimental results of melanin inhibition rate are shown in Table 7 below (mean ± standard deviation).

[0107] Table 7

[0108]

[0109] The experimental results of tyrosinase inhibition rate are shown in Table 8 below (mean ± standard deviation).

[0110] Table 8

[0111]

[0112] The results showed that in the Grasse rose extract obtained by the flash extraction method in Example 1, the ratio of astragaloside, ellagic acid and isoquercetin was closest to the ratio with the best whitening effect, and the melanin inhibition rate and tyrosinase inhibition rate of B16-F10 cells were the highest. However, the above effects could not be achieved by microwave thermal reflux, ultrasonic extraction and other methods.

[0113] Example 2

[0114] Weigh 5 g of naturally air-dried Grasse rose flowers and crush them. Add 350 mL of 30% 1,3-butanediol aqueous solution at a solid-liquid ratio of 1:70, perform flash extraction for 30 s (voltage 80 V), filter the extract through a 0.45 μm filter plate, take half of it and freeze-dry it to obtain Grasse rose extract 2.

[0115] Example 3

[0116] Compared with Example 2, the only difference is that the voltage of the flash extraction is changed to 100V, and the rest is the same.

[0117] The obtained extract was designated as Grasse rose extract 3.

[0118] Example 4

[0119] Compared with Example 2, the only difference is that 300 mL of 30% 1,3-butanediol aqueous solution is added at a material-liquid ratio of 1:60, and the rest is the same.

[0120] The obtained extract was designated as Grasse rose extract 4.

[0121] Example 5

[0122] Compared with Example 2, the only difference is that 400 mL of 30% 1,3-butanediol aqueous solution is added at a material-liquid ratio of 1:80, and the rest is the same.

[0123] The obtained extract was designated as Grasse rose extract 5.

[0124] Example 6

[0125] Compared with Example 2, the only difference is that the flash extraction time is changed to 40s, and the rest is the same.

[0126] The obtained extract was designated as Grasse rose extract 6.

[0127] Example 7

[0128] Compared with Example 2, the only difference is that the flash extraction time is changed to 20s, and the rest is the same.

[0129] The obtained extract was designated as Grasse rose extract 7.

[0130] Example 8

[0131] Compared with Example 2, the only difference is that the concentration of the 1,3-butanediol aqueous solution is changed to 40%, and the rest is the same.

[0132] The obtained extract was designated as Grasse rose extract 8.

[0133] Example 9

[0134] Compared with Example 2, the only difference is that the concentration of the 1,3-butanediol aqueous solution is changed to 20%, and the rest is the same.

[0135] The obtained extract was designated as Grasse rose extract 9.

[0136] Comparative Example 4

[0137] Compared with Example 2, the only difference is that 1,3-butanediol is replaced by ethanol, and the rest is the same.

[0138] The obtained extract was designated as Grasse rose extract D4.

[0139] The contents of ellagic acid, isoquercetin and astragaloside in Grasse rose extracts 1 to 9 and Grasse rose extract D4 were detected by HPLC. The test results are shown in Table 9 below (mean ± standard deviation):

[0140] Table 9

[0141] Ellagic acid (mg / g) Isoquercetin(mg / g) Astragaloside(mg / g) Grasse Rose Extract 1 69.88±0.11 34.19±0.62 102.61±0.74 Grasse Rose Extract 2 94.88±0.11 47.19±0.62 141.61±0.74 Grasse Rose Extract 3 72.13±0.29 36.01±0.11 107.18±0.21 Grasse Rose Extract 4 67.51±0.39 31.29±0.15 99.13±0.12 Grasse Rose Extract 5 61.31±0.26 30.12±0.12 92.71±0.21 Grasse Rose Extract 6 53.41±0.19 24.13±0.13 79.11±0.41 Grasse Rose Extract 7 57.34±0.31 27.21±0.21 86.10±0.16 Grasse Rose Extract 8 50.27±0.23 26.12±0.31 53.17±0.62 Grasse Rose Extract 9 63.61±0.15 21.41±0.16 23.63±0.15 Grasse Rose Extract D4 34.55±0.15 26.22±0.48 67.34±0.51

[0142] The results showed that the contents of astragalus glycoside, ellagic acid and isoquercetin in Grasse rose extract 2 were significantly increased and met the optimal ratio.

[0143] In summary, when the ratio of astragaloside, ellagic acid, and isoquercetin is 3:2:1, the melanin inhibition rate and tyrosinase inhibition rate of B16-F10 cells are the highest. When the Grasse rose extract is obtained by the preparation process in Example 2, the astragaloside, ellagic acid, and isoquercetin in the extract meet the best ratio for whitening effect, and the content of these three monomers is significantly improved compared with other embodiments. The present invention determines for the first time the best ratio of astragaloside, ellagic acid, and isoquercetin in rose monomers for whitening effect, and provides a new preparation process for Grasse rose extract, namely flash extraction, and the monomers in the Grasse rose extract using this preparation process meet the optimal ratio for whitening effect, and the content has been significantly improved, providing a cosmetic raw material for Grasse rose extract with whitening effect, providing a basis for its application in cosmetics.

[0144] Finally, it should be noted that the above content is only used to illustrate the technical solution of the present invention, rather than to limit the scope of protection of the present invention. Simple modifications or equivalent substitutions of the technical solution of the present invention by ordinary technicians in this field do not deviate from the essence and scope of the technical solution of the present invention.

Claims

1. A method for preparing Grasse rose extract, characterized in that: The following steps are involved: Grasse rose is mixed with solvent and flash extracted to obtain Grasse rose extract; Wherein, the solvent is a butanediol aqueous solution.

2. The preparation method according to claim 1, characterized in that: The Grasse rose is dried Grasse rose; and the butanediol is 1,3-butanediol.

3. The preparation method according to claim 1, characterized in that: The butanediol aqueous solution is a butanediol aqueous solution with a weight concentration of 20%-40%; the solid-liquid ratio of the Grasse rose to the solvent is 1:60-80, and the unit is g / mL.

4. The preparation method according to claim 1, characterized in that: The time of the flash extraction is 20-40s, and the voltage of the flash extraction is 80-100V.

5. The preparation method according to claim 4, characterized in that: The time of the flash extraction is 30s, and the voltage of the flash extraction is 80V.

6. The preparation method according to claim 1, characterized in that: The Grasse rose is crushed before being mixed with the solvent, and filtered and dried after the flash extraction.

7. The preparation method according to claim 6, characterized in that: The filtration is filter plate filtration, and the drying is freeze drying.

8. Grasse rose extract obtained by the preparation method according to any one of claims 1 to 7.

9. Use of the Grasse rose extract obtained by any one of the preparation methods of claims 1 to 7 in cosmetics.

10. A cosmetic, characterized in that: The invention relates to a Grasse rose extract prepared by the preparation method according to any one of claims 1 to 7.

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