Oil-control acne-removal composition

Abstract

The invention discloses an oil-controlling and acne-removing composition, and belongs to the field of cosmetics. The composition is prepared from a pueraria mirifica extract, an artemisia annua extract, sarcosine and an asparagus fern extract. According to the composition provided by the invention, all the raw materials are compounded for use and have a synergistic effect, so that excellent oil-controlling and acne-removing effects are obtained.

Description

Technical Field

[0001] The present application relates to the field of cosmetics, and in particular to an oil-controlling and acne-removing composition. Background Art

[0002] Acne is a common skin problem, mainly caused by excessive sebum secretion, abnormal keratinization of hair follicles, infection with Propionibacterium acnes and inflammatory response. Although there are many kinds of existing acne removal products, they often have problems such as strong irritation, insignificant effect or dependence on hormones, which makes it difficult to meet consumers' demand for gentle and efficient acne removal products.

[0003] In recent years, natural plant extracts have gradually become an important ingredient in anti-acne cosmetics due to their mildness, safety and diverse biological activities. For example, green tea extract, white willow bark extract, and Phellodendron bark extract have been widely used in anti-acne products, with significant antibacterial, anti-inflammatory and oil-control effects. In addition, some plant extracts can also regulate the balance of skin microecology and reduce the recurrence of acne.

[0004] The formula design of anti-acne cosmetics should follow the following principles: fast onset and high efficiency; avoid the use of banned ingredients such as antibiotics and hormones, and use natural ingredients to the maximum extent; at the same time, focus on repairing the skin and improving skin immunity. The formula design ideas include removing surface keratin, unblocking the oil secretion pathway, adding oil control ingredients, inhibiting excessive oil secretion, and anti-inflammatory and antioxidant.

[0005] Although a variety of plant extracts have been used in acne-removing products, existing products still have certain limitations. For example, some products are not effective enough in removing acne or are not gentle enough on sensitive skin. Therefore, there is an important market demand for developing a natural plant composition that can both effectively remove acne and gently care for the skin. Summary of the invention

[0006] The purpose of the present invention is to provide an oil-controlling and acne-removing composition, which comprises pueraria root extract, artemisia annua extract, sarcosine and asparagus spinulosa extract, has high safety and excellent oil-controlling and acne-removing effects.

[0007] In order to achieve the above object, the present invention adopts the following technical solutions:

[0008] In a first aspect, the present invention provides an oil-controlling and anti-acne composition, comprising the following raw materials: Pueraria lobata extract, Artemisia annua extract, sarcosine, and Asparagus spinulosa extract.

[0009] Preferably, the oil control and acne removal composition comprises the following raw materials in parts by weight:

[0010] Pueraria root extract: 0.5-2 parts;

[0011] Artemisia annua extract: 0.1-2 parts;

[0012] Creatine: 1-10 parts;

[0013] Asparagus spinulosa extract: 0.2-5 parts.

[0014] Furthermore, the oil-controlling and anti-acne composition comprises the following raw materials in parts by weight:

[0015] Pueraria root extract: 1.3-2 parts;

[0016] Artemisia annua extract: 1.2-2 parts;

[0017] Creatine: 5-10 parts;

[0018] Asparagus spinulosa extract: 2.5-5 parts.

[0019] Most preferably, the oil control and acne removal composition comprises the following raw materials in parts by weight:

[0020] Pueraria root extract: 1 part;

[0021] Artemisia annua extract: 1 part;

[0022] Creatine: 5 parts;

[0023] Asparagus spinulosa extract: 2.7 parts.

[0024] In a second aspect, the present invention provides an essence, wherein the essence comprises the following components in percentage by weight:

[0025] The oil-controlling and anti-acne composition of the first aspect: 1-10 wt %;

[0026] Emulsifier: 1-5wt%;

[0027] Humectant: 10-12wt%;

[0028] Emollient: 1-5wt%;

[0029] Deionized water was added to make up to 100 wt%.

[0030] Preferably, the emulsifier is one or more of polysorbate 20, polysorbate 80, PEG-40 hydrogenated castor oil, and lecithin; the moisturizer is one or more of glycerin, hyaluronic acid, propylene glycol, butylene glycol, and panthenol; and the emollient is one or more of squalane, olive oil, polydimethylsiloxane, and grape seed oil.

[0031] In a third aspect, the present invention provides a method for preparing an essence, the specific steps of which are as follows:

[0032] S1: Mix 1 / 3 of deionized water and moisturizer evenly to obtain a mixed solution A;

[0033] S2: Evenly mix 1 / 3 of deionized water with an emulsifier and a moisturizer to obtain a mixed solution B;

[0034] S3: Evenly mix 1 / 3 of deionized water, the oil-controlling and anti-acne composition of the first aspect and the mixed solution A, and homogenize for 0.5-1h to obtain a mixed solution C;

[0035] S4: Mix the mixed solution B and the mixed solution C evenly, homogenize for 0.5-1h, fill, sterilize, seal, protect from light, and store at room temperature.

[0036] Compared with the prior art, the present invention has the following beneficial effects: the present invention provides an oil-controlling and acne-removing composition, which is compounded by Pueraria lobata extract, Artemisia annua extract, sarcosine, and Asparagus spinulosa extract. The Pueraria lobata extract has anti-inflammatory, anti-oxidative and skin metabolism regulating effects, and can reduce acne and inflammatory reactions around acne by inhibiting the generation of inflammatory factors, and can also promote skin metabolism and help repair damaged skin barriers; Artemisia annua extract can reduce the accumulation of oil on the skin surface by inhibiting the oil secretion of sebaceous gland cells, and also has a certain antibacterial effect, which can inhibit the proliferation of Propionibacterium acnes; sarcosine can reduce excessive secretion of skin oil; Asparagus spinulosa can reduce skin inflammatory reactions, reduce the redness and swelling around acne, regulate the skin microenvironment, and reduce oil secretion. Among the above raw materials, Pueraria lobata extract and Asparagus spinulosa extract reduce oil secretion by regulating skin metabolism and water-oil balance, while Artemisia annua extract can directly inhibit the oil secretion of sebaceous gland cells, further synergistically controlling oil; Artemisia annua extract and Asparagus spinulosa extract reduce possible inflammatory responses by inhibiting the growth of Propionibacterium acnes and reducing the production of inflammatory factors, thereby playing a synergistic antibacterial and anti-inflammatory role.

[0037] The research results show that the combined use of Pueraria lobata extract, Artemisia annua extract, sarcosine and Asparagus spinulosa extract has a significant synergistic effect and can significantly control oil and remove acne. DETAILED DESCRIPTION

[0038] In order to better understand the present invention, the present invention is further described below in conjunction with specific application examples, wherein the terms used in the application examples are for describing specific implementation schemes and do not constitute limitations on the protection scope of the present invention.

[0039] The experimental methods in the following examples without specifying specific conditions are usually carried out under conventional conditions or conditions recommended by the manufacturer. Unless otherwise specified, the calculation is based on mass percentage and mass fraction.

[0040] Some raw materials and their sources are as follows:

[0041] Pueraria lobata extract: purchased from Xi'an Youshuo Biotechnology Co., Ltd. and Beijing Oriental Miaosen Biotechnology Co., Ltd.

[0042] Artemisia annua extract: purchased from Yunnan Yingge Biotechnology Co., Ltd.

[0043] Sarcosine: purchased from Jiahe Biotechnology Co., Ltd.

[0044] Asparagus spinulosa extract: purchased from Guangzhou Baihaobo Co., Ltd.

[0045] Other raw materials and reagents are commercially available.

[0046] The mass proportions of the raw materials in the oil-controlling and anti-acne composition are as follows:

[0047] Composition 1:

[0048] Pueraria root extract: 1 part, Artemisia annua extract: 1 part, Sarcosine: 5 parts, Asparagus spinulosa extract 2.7 parts.

[0049] Composition 2:

[0050] Pueraria root extract: 0.5 parts, Artemisia annua extract: 2 parts, Sarcosine: 1 part, Asparagus spinulosa extract 5 parts.

[0051] Composition 3:

[0052] Pueraria root extract: 2 parts, Artemisia annua extract: 0.1 parts, Sarcosine: 10 parts, Asparagus spinulosa extract 0.2 parts.

[0053] Composition ①:

[0054] The difference from composition 1 is that the Pueraria lobata extract is missing, and the missing part is made up with Artemisia annua extract, sarcosine and Asparagus spinulosa extract in a mass ratio of 1:5:2.7.

[0055] Composition ②:

[0056] The difference from composition 1 is that the Artemisia annua extract is missing, and the missing part is made up by Pueraria lobata extract, sarcosine and Asparagus spinulosa extract in a mass ratio of 1:5:2.7.

[0057] Composition ③:

[0058] The difference from composition 1 is that sarcosine is lacking, and the lacking mass fraction is made up by using pueraria root extract, artemisia annua extract and asparagus spinulosa extract in a mass ratio of 1:1:2.7.

[0059] Composition ④:

[0060] The difference from composition 1 is that the Asparagaceae spinulosa extract is missing, and the missing part is made up with Pueraria lobata extract, Artemisia annua extract and sarcosine in a mass ratio of 1:1:5:2.

[0061] Composition ⑤:

[0062] Pueraria lobata extract: 5 parts, Artemisia annua extract: 3.8 parts, Sarcosine: 0.8 parts, Asparagus spinulosa extract: 0.1 parts.

[0063] The mass percentages of the components in the essence are as follows:

[0064] Table 1 Essence components and their mass percentages

[0065]

[0066]

[0067] The emulsifier is polysorbate-20, polysorbate-80, and PEG-40 hydrogenated castor oil, and the mass ratio of polysorbate-20, polysorbate-80, and PEG-40 hydrogenated castor oil is 1:0.3:0.4;

[0068] The moisturizing agent is glycerin and panthenol, and the mass ratio of glycerin to panthenol is 1:0.5;

[0069] The emollients are olive oil and grape seed oil, and the mass ratio of the olive oil to the grape seed oil is 1:1.

[0070] The preparation methods of the above application examples 1-5 are as follows:

[0071] S1: Mix 1 / 3 of deionized water and moisturizer evenly to obtain a mixed solution A;

[0072] S2: Evenly mix 1 / 3 of deionized water with an emulsifier and a moisturizer to obtain a mixed solution B;

[0073] S3: 1 / 3 of deionized water, the oil-controlling and anti-acne composition and the mixed solution A are mixed evenly, and homogenized for 1 hour to obtain a mixed solution C;

[0074] S4: Mix the mixed solution B and the mixed solution C evenly, homogenize for 1 hour, fill, sterilize, seal, protect from light, and store at room temperature.

[0075] The preparation method of the blank application example is as follows:

[0076] S1: Mix 1 / 3 of deionized water and moisturizer evenly to obtain a mixed solution A;

[0077] S2: Evenly mix 1 / 3 of deionized water with an emulsifier and a moisturizer to obtain a mixed solution B;

[0078] S3: Mix 1 / 3 of deionized water and mixed solution A evenly, and homogenize for 1 hour to obtain mixed solution C;

[0079] S4: Mix the mixed solution B and the mixed solution C evenly, homogenize for 1 hour, fill, sterilize, seal, protect from light, and store at room temperature.

[0080] Test 1: Patch test:

[0081] The present invention experiments verify the biosafety of application examples 1-5 and blank application examples.

[0082] The 2015 "Technical Specifications for Safety of Cosmetics" was used as a reference standard for the irritation evaluation of cosmetics. The test method was a skin patch test, and 60 people aged 16-65 years were randomly distributed and tested.

[0083] Test method: Put the test substance into the patch tester, the dosage is 0.020-0.025g, cover the patch tester with the test substance on the back or forearm of the subject with non-irritating cloth-based tape, and gently press it with the palm to make it evenly adhere to the skin surface for 24 hours. After removing the test patch tester, observe the skin reaction after the indentation disappears 30 minutes later. If the result is negative, observe again 24 hours and 48 hours after the patch test.

[0084] Evaluation criteria:

[0085] Grade 0: negative reaction;

[0086] Grade 1: Suspicious reaction, only slight erythema;

[0087] Grade 2: weak positive reaction, erythema, infiltration, edema, and papules may be present;

[0088] Grade 3: Strong positive reaction, erythema, infiltration, edema, papules, and the reaction may extend beyond the test area;

[0089] Grade 4: Very strong positive reaction, obvious erythema, severe infiltration, edema, confluent herpes, and reaction beyond the test area.

[0090] Test results: Skin reactions were negative in all subjects.

[0091] Test 2: Evaluation of oil control efficacy

[0092] Test samples: compositions 1-3, compositions ①-⑤.

[0093] Preparation of test samples: Each test sample was prepared into a sample solution with a concentration of 5 wt % using Tris-HCl (pH=7.0) buffer.

[0094] Principle of the test: 5α-reductase is a membrane protease located in the microsomes and cell nuclei. It uses the reduced coenzyme NADPH as a hydrogen donor to catalyze the conversion of testosterone into dihydrotestosterone, which can induce excessive oil secretion of sebaceous glands. By inhibiting the activity of 5α-reductase and reducing the production of dihydrotestosterone, the level of sebaceous gland lipid secretion is reduced. Using the 5α-reductase inhibitory activity as an indicator, the inhibitory effect of the product on 5α-reductase is explored through experimental methods to evaluate the oil control effect of the product.

[0095] Test method: 1mL reaction system contains 100μmol / L NADPH, 5μmol / L testosterone, 0.6mg / L 5α-reductase and 200μL prepared sample solution, and the balance is supplemented with Tris-HCl buffer; the blank group uses Tris-HCl (pH=7.0) buffer to replace the prepared sample solution. The reaction system reacts at 37°C for 10min, and the absorbance of the sample group and the blank group at 0min and 10min at OD340nm is measured respectively. The inhibition rate of the test sample on 5α-reductase is calculated according to the following formula, and the average is taken after three times to evaluate the inhibitory effect of the test sample on 5α-reductase. The results are shown in Table 2, and the test results are expressed as mean ± SD.

[0096] 5α-reductase inhibition rate = [(B 0 -B 10 )-(A 0 -A 10 )] / (B 0 -B 10 )×100%

[0097] In the above formula: A 0 -The absorbance of the sample group at 0 min reaction time;

[0098] A 10 - Absorbance of the sample group after 10 min of reaction;

[0099] B 0 -The absorbance of the blank group at 0 min reaction time;

[0100] B 10 -The absorbance of the blank group after 10 min of reaction.

[0101] Experiment 3: Evaluation of acne-removing efficacy

[0102] Test samples: compositions 1-3, compositions ①-⑤.

[0103] Preparation of test samples: The test samples were prepared into sample solutions with a concentration of 5 wt % using deionized sterile water.

[0104] Test method: Activate the Propionibacterium acnes strain and dilute it to 1.0x10 6 CFU / mL, 100 μL of diluted bacterial solution and 20 μL of sample solution were added to each well, and the blank group used an equal amount of deionized sterile water instead of sample solution. The 96-well plate with sample solution and bacterial solution was placed in an anaerobic box for incubation for 48 hours and then taken out. The OD600nm of each well was detected by an ELISA instrument, and the bacterial content in each well system was determined according to the absorbance, and the inhibition rate of Propionibacterium acnes was calculated according to the following formula. The results are shown in Table 2, and the test results are expressed as mean ± SD.

[0105] The culture medium required for activation and cultivation of Propionibacterium acnes is Propionibacterium acnes liquid culture medium purchased from Shifeng Biotechnology.

[0106] Inhibition rate % = (OD blank - OD sample) / OD blank

[0107] OD blank is the OD value of the blank group;

[0108] OD sample is the OD value of the sample group.

[0109] Table 2 Evaluation results of the oil control and acne removal effects of the compositions

[0110]

[0111]

[0112] Note: “*” indicates that compared with composition 1, p<0.05.

[0113] According to the results in Table 2, compositions 1-3 have significant oil control and acne removal effects. By comparing the results of composition 1 with compositions ①-④, it can be seen that there is a significant synergistic effect between the Pueraria lobata extract, Artemisia annua extract, sarcosine and Asparagus spinulosa extract specified in the present invention; by comparing the results of composition 1 with composition ⑤, it can be seen that when the dosage ratio of Pueraria lobata extract, Artemisia annua extract, sarcosine and Asparagus spinulosa extract is within the range specified in the present invention, the oil control and acne removal effect of the composition is more significant.

[0114] Experiment 4: Human body oil control efficacy test

[0115] Thirty volunteers with oily facial skin (aged 18-26) were recruited and divided into 6 groups, with 5 people in each group. They were smeared with the essence of application examples 1-5 and blank application example respectively. They were smeared on the face once in the morning and evening after cleansing, with a single application of 2 mL for 30 days. The results are shown in Table 3:

[0116] 30-day oil reduction rate = (measured value on day 0 - measured value on day 30) / measured value on day 0 × 100%.

[0117] The measured values ​​are obtained by measuring the skin of a fixed area using a skin oil tester (Sebum Scale).

[0118] Table 3 Human body oil control efficacy test results

[0119]

[0120] As shown in Table 3 above, Application Examples 1-5 have significant oil control effects compared to the blank application example.

[0121] The embodiments described above are part of the embodiments of the present application, rather than all of the embodiments. The detailed description of the embodiments of the present application is not intended to limit the scope of the present application for protection, but merely represents the selected embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without making creative work are within the scope of protection of the present application.

Claims

1. An oil-controlling and acne-removing composition, characterized in that: Including the following raw materials by weight: Pueraria root extract: 0.5-2 parts; Artemisia annua extract: 0.1-2 parts; Creatine: 1-10 parts; Asparagus spinulosa extract: 0.2-5 parts.

2. The composition according to claim 1, characterized in that Including the following raw materials by weight: Pueraria root extract: 1.3-2 parts; Artemisia annua extract: 1.2-2 parts; Creatine: 5-10 parts; Asparagus spinulosa extract: 2.5-5 parts.

3. The composition according to claim 2, characterized in that Including the following raw materials by weight: Pueraria root extract: 1 part; Artemisia annua extract: 1 part; Creatine: 5 parts; Asparagus spinulosa extract: 2.7 parts.

4. Use of the oil-controlling and anti-acne composition according to any one of claims 1 to 3 in preparing a skin care product having any one or more of the functions of oil-controlling, anti-acne, anti-oxidation, antibacterial, anti-inflammatory, and soothing.

5. The use according to claim 4, characterized in that: The skin care product is any one of essence, lotion and cream.

6. An essence as claimed in claim 5, characterized in that: Contains the following components by mass percentage: The oil-controlling and anti-acne composition according to any one of claims 1 to 3: 1-10 wt %; Emulsifier: 1-5wt%; Humectant: 10-12wt%; Emollient: 1-5wt%; Deionized water was added to make up to 100 wt%.

7. The essence according to claim 6, characterized in that: The emulsifier is one or more of polysorbate-20, polysorbate-80, PEG-40 hydrogenated castor oil, and lecithin.

8. The essence according to claim 6, characterized in that: The moisturizing agent is one or more of glycerin, hyaluronic acid, propylene glycol, butylene glycol, and panthenol.

9. The essence according to claim 6, characterized in that: The emollient is one or more of squalane, olive oil, polydimethylsiloxane, and grape seed oil.

10. A method for preparing the essence according to any one of claims 6 to 9, characterized in that: The method comprises the following preparation steps: S1: Mix 1 / 3 of deionized water and moisturizer evenly to obtain a mixed solution A; S2: Evenly mix 1 / 3 of deionized water with an emulsifier and a moisturizer to obtain a mixed solution B; S3: Evenly mix 1 / 3 of deionized water, the oil-controlling and anti-acne composition and the mixed solution A, and homogenize for 0.5-1h to obtain a mixed solution C; S4: Mix the mixed solution B and the mixed solution C evenly, homogenize for 0.5-1h, fill, sterilize, seal, protect from light, and store at room temperature.

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