Pretreatment instrument for sample analysis

Abstract

The invention discloses a pretreatment instrument for sample analysis. The pretreatment instrument comprises a rack, an XYZ moving module, a three-axis manipulator and a pipetting device, a placing area, a feeding area, a uniform mixing area and a centrifugal area are arranged on the rack, and the placing area is located at one end corner of the rack; the centrifugal area is located on one side of the rack. The feeding area is located between the containing area and the centrifugal area and provided with a salt adding module. The uniform mixing area is positioned at the front end of the placing area; the salt adding module comprises a base, a driving assembly, a storage barrel and a feeding assembly, a discharging opening is formed in the bottom end of the storage barrel, the feeding assembly comprises a feeding shaft, a fixing frame and a discharging pipe, the feeding shaft is horizontally and transversely arranged in the fixing frame in a rotating mode, the feeding shaft comprises a rod body part and a bearing part, and a plurality of feeding grooves are formed in the surface of the bearing part; the bearing part is located at the bottom end of the discharging port. The device has the advantages that all the modules are reasonably arranged, the experiment efficiency can be improved, the salt adding module can accurately control the salt adding amount, and experiment errors can be reduced.

Description

Technical Field

[0001] The invention relates to the technical field of chemical sample processing instruments, and in particular to an instrument for sample pre-analysis processing. Background Art

[0002] QuECHERS (Quick, Easy, Cheap, Rugged, Safe) is a rapid sample pretreatment technology for agricultural product testing that has been developed internationally in recent years. The principle is similar to that of high-performance liquid chromatography and solid phase extraction, both of which use the interaction between adsorbent fillers and impurities in the matrix to adsorb impurities to achieve the purpose of impurity removal and purification. Specifically, the homogenized sample is extracted with acetonitrile, and then the sample is separated by salting out and stratified using extraction salt. The matrix dispersed extraction mechanism is used, and PSA or other adsorbents are combined with most of the interfering substances (organic acids, fatty acids, carbohydrates, etc.) in the matrix, and removed by centrifugation to achieve the purpose of purification.

[0003] In order to improve the efficiency of sample pre-analysis treatment, a QuECHERS experimental device has emerged, including a salting module, a homogenizer module, a liquid adding module, an oscillation module, a centrifugal module, and a vortex mixing module. By setting up an XYZ three-axis module, the multifunctional robotic arm can be moved forward and backward, left and right, and up and down, thereby ensuring that the multifunctional robotic arm can grab the sample tube or purification tube or finished product tube and move it to a suitable position in multiple directions, thereby reducing manual handling. After the sample tube, purification tube, or finished product tube is moved to a suitable position, the sample is collected for liquid extraction, centrifugal separation, solid phase extraction, and nitrogen blowing concentration. This improves the degree of automation of the entire experiment, reduces manual operation, and saves experimental time.

[0004] However, the modules in the existing sample analysis pretreatment equipment are arranged in a chaotic manner, and the XYZ three-axis module requires a large span to drive the multifunctional robotic arm to move, which results in low efficiency in moving the sample tube during the experiment; and when the salt adding module adds salt to the sample tube, it is difficult to control the amount of salt added, which makes the concentration of the supernatant in the sample tube prone to experimental errors, and improvements need to be made. Summary of the invention

[0005] The purpose of the present invention is to provide an instrument for sample pre-analysis treatment, in which the positions of the modules are reasonably arranged and the salt adding module can accurately control the amount of salt added, thereby reducing experimental errors.

[0006] The present invention provides an instrument for sample analysis pretreatment, comprising a frame, an XYZ moving module, a three-axis manipulator and a liquid transfer device, wherein the XYZ moving module drives the three-axis manipulator and the liquid transfer device to perform XYZ three-dimensional movement; The rack is provided with a placement area, a feeding area, a mixing area and a centrifugal area, wherein the placement area is located at one end corner of the rack, and the placement area is provided with a refrigeration module, a centrifugal tube rack for placing sample tubes and purification tubes, and a gun tip rack for placing disposable gun tips; The centrifugal area is located on one side of the frame, and a centrifuge is provided at the centrifugal area; The feeding area is located between the placement area and the centrifugal area. The feeding area is provided with a salt adding module, a homogenizing module, a liquid adding module, a cover switch device and a translation module along its length direction. The translation module is provided with a bottle body fixing module for fixing the sample tube; The mixing zone is located at the front end of the placement zone, and the mixing zone is provided with a vortex module and a vertical oscillation module along its length direction; The salt adding module includes a base, a driving assembly, a storage barrel and a feeding assembly. A discharge port is formed at the bottom end of the storage barrel. The feeding assembly includes a feeding shaft, a fixing frame and a discharge pipe. The feeding shaft is horizontally and laterally rotatably arranged in the fixing frame. The feeding shaft includes a rod body and a bearing portion. A plurality of feed grooves are formed on the surface of the bearing portion. The bearing portion is located at the bottom end of the discharge port, and the discharge pipe is arranged at the bottom end of the bearing portion.

[0007] Preferably, the driving assembly includes a driving motor, a rotating shaft and a driving gear. The rotating shaft is rotatably installed on the base and the storage barrel. The driving motor drives the rotating shaft to rotate. The driving gear is fixed on the rotating shaft. A driven gear meshing with the driving gear is fixed on the rod body.

[0008] Preferably, the driving assembly also includes a driving wheel, a driven wheel, a synchronous belt and a mounting frame, the driving wheel is fixedly set on the rotating shaft of the driving motor, the mounting frame is fixedly set on the rotating shaft, the driven wheel is fixedly set on the periphery of the mounting frame, and the synchronous belt is wound around the driving wheel and the driven wheel.

[0009] Preferably, one end of the rotating shaft is inserted into the storage barrel, and a stirring assembly is provided on the rotating shaft, and the stirring assembly includes a stirring handle and a plurality of stirring blades. The stirring handle is relatively fixed to the rotating shaft, and the plurality of stirring blades are fixed on the peripheral side of the stirring handle. The rotating shaft rotates to drive the stirring handle and the stirring blades to rotate.

[0010] Preferably, four stirring blades are provided in the stirring assembly, and two adjacent stirring blades are perpendicular to each other. The four stirring blades are fixed to each other and enclose a through groove. A protrusion is provided at the bottom end of the stirring handle, and the protrusion is embedded in the through groove, and the peripheral side of the protrusion abuts against the inner wall of the through groove.

[0011] Preferably, the stirring blade abuts against the bottom surface of the material storage barrel, and the blade surface of the stirring blade faces downward.

[0012] Preferably, the XYZ mobile module includes an X-axis component, a Y-axis component and a Z-axis component, and the Z-axis component includes a first lifting motor and a second lifting motor. The first lifting motor drives the three-axis manipulator to perform lifting motion, and the second lifting motor drives the pipetting device to perform lifting motion.

[0013] Preferably, the bottle body fixing module comprises a bottle body placing platform and a bottle body clamping cylinder, and the bottle body clamping cylinder is located above the bottle body placing platform.

[0014] Preferably, the switch cover device includes a frame plate, a vertical rodless cylinder, a mounting plate, a plurality of vertical rodless cylinders and a bottle cap rotating module, the vertical rodless cylinder is fixedly arranged on the frame plate and drives the mounting plate to rise and fall, a plurality of the vertical rodless cylinders are vertically fixedly arranged on the mounting plate and drive the bottle cap rotating module to rise and fall; the vertical rodless cylinder includes a cylinder barrel, a slide, an upper end cover and a lower end cover, the outer sleeve of the cylinder barrel is provided with a pre-stressed spring, the pre-stressed spring is located between the slide and the upper end cover, and the bottle cap rotating module is fixed to the surface of the slide; the bottle cap rotating module includes a rotating jaw, a plurality of clamping blocks and a driving unit for driving the rotating jaw to rotate, a plurality of the clamping blocks are fixedly arranged at the bottom end of the jaws of each rotating jaw, the clamping blocks are in the shape of a plurality of steps, the inner wall surface of the clamping blocks is formed into a plurality of layers of arcuate surfaces, and the diameter of the arcuate surface of the inner wall of the clamping block decreases step by step toward the rotating jaw.

[0015] Preferably, the clamping block comprises a first clamping portion, a second clamping portion and a fixing portion, the first clamping portion is located at the upper end of the second clamping portion, and the diameter of the arcuate surface of the inner side wall of the first clamping portion is smaller than the diameter of the arcuate surface of the inner side wall of the second clamping portion.

[0016] Preferably, the fixing portion is located outside the first clamping portion, a transition block is provided between each clamping block and the jaws of the rotating clamp, and two ends of the transition block are fixedly connected to the fixing portion and the jaws of the rotating clamp respectively.

[0017] Preferably, a buffer spring is provided on the outer sleeve of the cylinder, and the buffer spring is located between the slide and the lower end cover.

[0018] Preferably, the bottle cap rotation module further comprises a fixing plate, the fixing plate is respectively fixed to the slide table and the rotating clamp, a sliding seat is arranged on the fixing plate, a sliding guide rail is vertically arranged on the mounting plate, and the sliding seat is clamped on the sliding guide rail.

[0019] Preferably, the liquid adding module comprises a liquid adding pipe and a liquid adding pump, the liquid adding pump is arranged at the bottom end of the frame, and the liquid adding pipe is located at the bottom end of the homogenizing submodule and is connected to the liquid adding pump.

[0020] From the above description of the present invention, it can be seen that the present invention has the following beneficial effects: 1. A discharge port is formed at the bottom of the storage barrel of the salt adding module. The salt particles in the storage barrel fall quantitatively into the feed slot of the feeding shaft from the discharge port. The rotating shaft drives the feeding shaft to rotate through the mutually meshing driving gear and driven gear, so that the salt particles can be quantitatively added to the sample bottle; thereby, the amount of salt particles added to the sample bottle can be accurately controlled, thereby reducing the error of the experimental results.

[0021] 2. The rack is divided into a placement area, a feeding area, a mixing area and a centrifugal area, so that each module is arranged reasonably and orderly in the pre-analysis processing equipment. During the experiment, the XYZ mobile module does not need to drive the three-axis manipulator to move with a large span, thereby making the three-axis manipulator have a higher moving efficiency for the sample tube during the experiment.

[0022] 3. In the sample analysis pretreatment instrument, the cover opening and closing device can open and close the cover of the sample tube by itself. There is no need to use the three-axis manipulator to assist in opening and closing the cover of the sample tube, which reduces unnecessary movement of the three-axis manipulator during the experiment and improves the overall experimental efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 This is a schematic diagram of the overall structure of an instrument for sample analysis pretreatment according to an embodiment; Figure 2 is a schematic structural diagram of the mobile module of Example XYZ; Figure 3 Schematic diagram of the positions of the placement area, feeding area, mixing area and centrifugation area of ​​the embodiment; Figure 4 is a top view of an instrument for sample analysis pretreatment according to an embodiment; Figure 5 It is a structural schematic diagram of a centrifuge tube rack and a gun tip rack in an embodiment; Figure 6 is a structural schematic diagram of a translation module of an embodiment; Figure 7 is a schematic structural diagram of a switch cover device of an embodiment; Figure 8 is a schematic structural diagram of a vertical rodless cylinder and a fixed plate of an embodiment; Fig. 9 is a schematic structural diagram of a bottle cap rotating module of an embodiment; Fig.10 is a schematic structural diagram of a clamping block in an embodiment; Fig.11 2 is a schematic diagram of the structure of a preload spring and a buffer spring in an embodiment; Fig.12 is a schematic structural diagram of a salt adding module in an embodiment; Fig.13 It is a schematic structural diagram of the discharge port of the embodiment; Fig.14 is a schematic diagram of the structure of a drive component of an embodiment; Fig.15 is a schematic structural diagram of the driving gear of the embodiment; Fig.16 is a schematic structural diagram of a stirring handle and a stirring blade in an embodiment; Fig.17 is a cross-sectional view of a feeding assembly of an embodiment; Fig.18 It is a schematic structural diagram of the feeding shaft of the embodiment; Fig.19 It is a schematic structural diagram of a liquid adding module according to an embodiment.

[0024] Figure numerals: 1, rack; 2, XYZ moving module; 21, X-axis assembly; 22, Y-axis assembly; 23, Z-axis assembly; 231, first lifting motor; 232, second lifting motor; 3, three-axis manipulator; 4, liquid transfer device; 5, placement area; 51, centrifuge tube rack; 511, sample tube; 512, purification tube; 52, gun head rack; 521, disposable gun head; 53, refrigeration module; 6, feeding area; 61, salting module; 611, base; 612, drive assembly; 6 121, driving motor; 6122, rotating shaft; 6123, driving gear; 6124, driving wheel; 6125, driven wheel; 6126, synchronous belt; 6127, mounting frame; 613, storage barrel; 6131, discharge port; 614, feeding assembly; 6141, feeding shaft; 61411, rod body; 61412, carrier; 61413, feeding trough; 6142, driven gear; 6143, fixing frame; 6144, discharge pipe; 615, stirring assembly; 61 51. stirring handle; 6152. stirring blade; 6153. through groove; 6154. protrusion; 62. homogenizing module; 63. liquid adding module; 631. liquid adding pump; 632. liquid adding pipe; 64. switch cover device; 641. shelf; 642. vertical rodless cylinder; 643. mounting plate; 644. vertical rodless cylinder; 6441. cylinder barrel; 6442. slide; 6443. upper end cover; 6444. lower end cover; 6445. preload spring; 6446. buffer spring; 645. Bottle cap rotation module; 6451. Rotating claw; 6452. Clamping block; 64521. First clamping part; 64522. Second clamping part; 64523. Fixing part; 64524. Arc surface; 6453. Driving unit; 6454. Fixing plate; 65. Translation module; 66. Bottle body fixing module; 661. Bottle body placement table; 662. Bottle body clamping cylinder; 7. Mixing zone; 71. Vortex module; 72. Vertical oscillation module; 8. Centrifugal zone; 81. Centrifuge. DETAILED DESCRIPTION

[0025] In order to make the technical problems, technical solutions and beneficial effects to be solved by the present invention clearer and more understandable, the following is a summary of the technical problems, technical solutions and beneficial effects to be solved by the present invention. Figure 1-19 It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0026] Reference Figure 1 and Figure 2 , a sample analysis pre-processing instrument, comprising a frame 1, an XYZ moving module 2, a three-axis manipulator 3 and a liquid transfer device 4, wherein the XYZ moving module 2 is arranged at the top of the frame 1, the XYZ moving module 2 comprises an X-axis assembly 21, a Y-axis assembly 22 and a Z-axis assembly 23, and the Z-axis assembly 23 comprises a first lifting motor 231 and a second lifting motor 232. The three-axis manipulator 3 is fixed to the telescopic rod of the first lifting motor 231, and the three-axis manipulator 3 is driven to rise and fall by the first lifting motor 231. The liquid transfer device 4 is fixed to the telescopic rod of the second lifting motor 232, and the liquid transfer device 4 is driven to rise and fall by the second lifting motor 232. The three-axis manipulator 3 and the liquid transfer device 4 are driven to move in the X direction by the X-axis assembly 21, and the three-axis manipulator 3 and the liquid transfer device 4 are driven to move in the Y direction by the Y-axis assembly 22, so that the XYZ moving module 2 can drive the three-axis manipulator 3 and the liquid transfer device 4 to move in the three directions of XYZ.

[0027] In order to enable the first lifting motor 231 to drive the first lifting seat to achieve lifting and sliding on the first lifting guide rail, and the second lifting motor 232 to drive the second lifting seat to achieve lifting and sliding on the second lifting guide rail. A first screw is fixedly arranged on the rotating shaft of the first lifting motor 231, and a second screw is fixedly arranged on the rotating shaft of the second lifting motor 232, and the first screw is threadedly connected to the first lifting seat, and the second screw is threadedly connected to the second lifting seat. The rotating shaft of the first lifting motor 231 rotates to drive the first screw to rotate, thereby realizing the lifting and lowering of the three-axis manipulator 3, and the rotating shaft of the second lifting motor 232 rotates to drive the second screw to rotate, thereby realizing the lifting and lowering of the liquid transfer device 4.

[0028] Reference Figure 3 , Figure 4 , Figure 5, a placement area 5, a feeding area 6, a mixing area 7 and a centrifugal area 8 are arranged on the rack 1, wherein the placement area 5 is located at one end corner of the rack 1, and a centrifugal tube rack 72651, a gun tip rack 52 and a refrigeration module 53 are arranged at the placement area 5, wherein the centrifugal tube rack 72651 is used to place sample tubes 511 and purification tubes 512, and a disposable gun tip 521 is placed on the gun tip rack 52. The feeding area 6 is located at one side end of the placement area 5, and the mixing area 7 is located at the front end of the placement area 5; the centrifugal area 8 is arranged on one side of the rack 1, so that the feeding area 6 is located between the centrifugal area 8 and the placement area 5, and a centrifuge 81 is arranged at the centrifugal area 8. A salt adding module 61, a homogenizing module 62, a liquid adding module 63, a switch cover device 64 and a translation module 65 are arranged in the feeding area 6. The salt adding module 61, the homogenizing module 62, the liquid adding module 63 and the switch cover device 64 are arranged along the width direction of the frame 1, and the switch cover device 64 is located at one end of the feeding area 6 close to the mixing area 7.

[0029] Reference Figure 1 , Figure 4 and Figure 6 , the translation module 65 is arranged at the bottom of the salt adding module 61, the homogenizing module 62 and the switch cover device 64, and a bottle body fixing module 66 for fixing the sample tube 511 is arranged on the translation module 65, and the bottle body fixing module 66 includes a bottle body placing table 661 and a bottle body clamping cylinder 662, wherein the bottle body placing table 661 is fixedly arranged above the translation module 65, and the bottle body clamping cylinder 662 is arranged above the bottle body placing table 661. The sample tube 511 is grasped by the three-axis manipulator 3 and placed on the bottle body placing table 661, and the sample tube 511 is clamped and fixed by the bottle body clamping cylinder 662, and the bottle body fixing module 66 is driven by the translation module 65 to move in the feeding area 6, so that the sample tube 511 fixed in the bottle body fixing module 66 can be moved to the bottom of the salt adding module 61, the homogenizing module 62, the liquid adding module 63 and the switch cover device 64.

[0030] Reference Figure 7 , Figure 8 and Fig. 9When the sample tube 511 fixed in the bottle body fixing module 66 moves to the bottom of the switch cover device 64, the bottle cap on the sample tube 511 is opened by the switch cover device 64. The switch cover device 64 includes a frame plate 641, a vertical rodless cylinder 642, a mounting plate 643, a pair of vertical rodless cylinders 644 and a pair of bottle cap rotating modules 645. The vertical rodless cylinder 642 is vertically fixed on the frame plate 641, and the mounting plate 643 is fixed to the vertical rodless cylinder 642. The mounting plate 643 is driven to rise and fall by the vertical rodless cylinder 642. The two vertical rodless cylinders 644 are vertically fixed on the mounting plate 643 to keep the two vertical rodless cylinders 644 parallel to each other. The vertical rodless cylinder 644 includes a cylinder barrel 6441, a slide 6442, an upper end cover 6443 and a lower end cover 6444. The upper end cover 6443 and the lower end cover 6444 are respectively located at the two ends of the cylinder barrel 6441, and the slide 6442 can slide on the cylinder barrel 6441.

[0031] The bottle cap rotating module 645 includes a rotating clamp 6451, a plurality of clamping blocks 6452, a driving unit 6453 and a fixing plate 6454. The fixing plate 6454 is fixed to the surface of the slide 6442, and a sliding seat is fixedly arranged on the surface of the fixing plate 6454. Correspondingly, a sliding guide rail is vertically fixedly arranged on the surface of the mounting plate 643, and the sliding seat is clamped on the sliding guide rail. The rotating clamp 6451 is fixed to the fixing plate 6454, and a transition block is fixedly arranged at the bottom end of each jaw of the rotating air claw by bolt locking, and a plurality of clamping blocks 6452 are fixedly arranged at the bottom end of each transition block.

[0032] Reference Fig. 9 and Fig.10The clamp block 6452 includes a first clamping portion 64521, a second clamping portion 64522 and a fixing portion 64523, wherein the first clamping portion 64521 is located at the top of the second clamping portion 64522, so that the clamp block 6452 is in a two-layer stepped shape; the fixing portion 64523 is located on one side of the first clamping portion 64521, and the fixing portion 64523 and the transition block are fixed by bolts, so that the clamp block 6452 is firmly installed. The inner surface of the first clamping portion 64521 and the inner surface of the second clamping portion 64522 are both arc-shaped surfaces 64524, and the diameter of the arc-shaped surface 64524 of the inner wall of the first clamping portion 64521 is smaller than the diameter of the arc-shaped surface 64524 of the inner wall of the second clamping portion 64522. Thus, the first clamping parts 64521 of each clamping block 6452 can be mutually enclosed to clamp a small-diameter bottle cap, and the second clamping parts 64522 of each clamping block 6452 can be mutually enclosed to clamp a large-diameter bottle cap. The setting of the transition block plays a transition role between the clamping block 6452 and the jaws of the rotating clamp 6451 to avoid interference between the clamping blocks 6452. The clamping block 6452 is set to a multi-layer stepped shape, so that the automatic opening and closing cover module has better adaptability to the opening of bottle caps with different calibers.

[0033] When it is necessary to open a small-diameter bottle cap, the vertical rodless cylinder 642 drives the mounting plate 643, the vertical rodless cylinder 644 and the bottle cap rotating module 645 to descend, so that the bottle cap rotating module 645 is close to the bottle cap. As the top surface of the small-diameter bottle cap abuts against the bottom end of each transition block, the rotating clamp 6451 drives each clamp block 6452 to enclose and approach each other, and the small-diameter bottle cap is clamped by the first clamping portion 64521 of each clamp block 6452. When it is necessary to open a large-diameter bottle cap, the top surface of the large-diameter bottle cap abuts against the bottom end of each first clamping portion 64521, and the rotating clamp 6451 drives each clamp block 6452 to enclose and approach each other, and the large-diameter bottle cap is clamped by the second clamping portion 64522 of each clamp block 6452. After the rotating clamping jaw 6451 drives each clamping block 6452 to clamp the bottle cap firmly, the driving unit 6453 drives the rotating clamping jaw 6451 and the bottle cap to rotate, thereby opening the bottle cap on the sample tube 511.

[0034] Reference Fig.11In order to make the height of the bottle cap rotating module 645 driven by the vertical rodless cylinder 642 after lifting and lowering, it can adapt to the centrifuge tubes of bottle caps of different calibers. A preload spring 6445 and a buffer spring 6446 are sleeved on the outer periphery of the cylinder 6441, wherein the preload spring 6445 is located between the slide 6442 and the upper end cover 6443, and the two ends of the preload spring 6445 are respectively in contact with the slide 6442 and the upper end cover 6443. The buffer spring 6446 is arranged between the slide 6442 and the lower end cover 6444, and the two ends of the buffer spring 6446 are respectively in contact with the slide 6442 and the lower end cover 6444. The pre-compression spring 6445 is provided to pre-compress the cap of the centrifuge tube. The slide 6442 compresses the pre-compression spring 6445 to enable each clamping block 6452 to adapt to the clamping of centrifuge tubes of different lengths and provide flexible jumping between the cap rotating module 645 and the centrifuge tube. The buffer spring 6446 is provided to buffer the descent of the cap rotating module 645, thereby preventing excessive pressure on the cap surface when the cap rotating module 645 descends.

[0035] Reference Fig.12 and Fig.13 When the bottle cap of the sample tube 511 is opened, it moves to the bottom of the salt adding module 61 under the driving action of the translation module 65, and salt is added to the sample tube 511 through the salt adding module 61. The salt adding module 61 includes a base 611, a driving component 612, a storage barrel 613, a feeding component 614 and a stirring component 615, wherein the storage barrel 613, the driving component 612 and the feeding component 614 are all arranged on the base 611, and the storage barrel 613 is arranged on the top of the driving component 612 and the feeding component 614. A discharge port 6131 is formed at the bottom of the storage barrel 613, and the salt particles in the storage barrel 613 are discharged from the discharge port 6131 under the action of gravity; the stirring component 615 is arranged in the storage barrel 613, and the salt particles in the storage barrel 613 are stirred, so that the salt particles located in the corners of the storage barrel 613 can be discharged from the discharge port 6131. The feeding assembly 614 is disposed at the bottom end of the discharge port 6131 so that the salt particles discharged from the discharge port 6131 are kept quantitative each time.

[0036] Reference Fig.14 and Fig.15The driving assembly 612 includes a driving motor 6121, a rotating shaft 6122, a driving gear 6123, a driving wheel 6124, a driven wheel 6125, a synchronous belt 6126 and a mounting frame 6127. The rotating shaft 6122 is vertically rotatably mounted on the base 611 and the material storage barrel 613. The mounting frame 6127 is mounted and fixed on the rotating shaft 6122, and the driven wheel 6125 is sleeved on the periphery of the mounting frame 6127 and fixed. The driving motor 6121 is fixed on the base 611, keeping the rotating shaft of the driving motor 6121 vertically upward, the driving wheel 6124 is fixed on the rotating shaft of the driving motor 6121, and finally the synchronous belt 6126 is wound around the driving wheel 6124 and the driven wheel 6125. The rotating shaft of the driving motor 6121 rotates, thereby driving the mounting frame 6127 and the rotating shaft 6122 to rotate through the driving wheel 6124, the synchronous belt 6126 and the driven wheel 6125.

[0037] Reference Fig.16 The stirring assembly 615 includes a stirring handle 6151 and four stirring blades 6152. The two adjacent stirring blades 6152 are kept perpendicular to each other, and the end of one stirring blade 6152 is fixed to the middle position of the other stirring blade 6152, so that the four stirring blades 6152 are mutually surrounded to form a through groove 6153. The blade surface of each stirring blade 6152 is kept downward, so that the four stirring blades 6152 are placed in the storage barrel 613 and abut against its bottom surface. Correspondingly, a protrusion 6154 is formed at the bottom end of the stirring handle 6151, so that the protrusion 6154 at the bottom end of the stirring handle 6151 is embedded in the through groove 6153, so that the outer surface of the protrusion 6154 abuts against the inner side wall of the through groove 6153. The rotating shaft 6122 rotates to drive the stirring handle 6151 to rotate. When the stirring handle 6151 rotates, the stirring blades 6152 are driven to rotate, thereby sweeping the salt particles in the storage barrel 613 to move and discharge them from the discharge port 6131 of the storage barrel 613.

[0038] Reference Fig.17 and Fig.18The feeding assembly 614 includes a feeding shaft 6141, a driven gear 6142, a fixing frame 6143 and a discharge pipe 6144. The fixing frame 6143 is fixedly arranged on the base 611 and located at the bottom end of the discharge port 6131. ​​The feeding shaft 6141 is horizontally rotatably arranged in the fixing frame 6143. The feeding shaft 6141 is composed of a rod body 61411 and a carrier part 61412. The carrier part 61412 is located at the bottom end of the discharge port 6131, and the circumference of the carrier part 61412 is in contact with the inner side wall of the fixing frame 6143. In addition, a plurality of feeding grooves 61413 are formed on the surface of the carrier part 61412. The driven gear 6142 is fixedly arranged at the end of the rod body 61411, and the driven gear 6142 is meshed with the driving gear 6123 of the driving assembly 612, so that the rotating shaft 6122 rotates to drive the feeding shaft 6141 to rotate. After the salt particles are discharged from the discharge port 6131 of the storage barrel 613, they enter the feeding trough 61413 of the carrier part 61412; the driving motor 6121 drives the rotating shaft 6122 to rotate at a constant speed, thereby also driving the powder feeding shaft to rotate at a constant speed, so that the amount of salt particles entering the feeding trough 61413 each time is equal. The discharge pipe 6144 is vertically arranged at the bottom end of the carrier part 61412 and fixed to the fixed frame 6143; the powder feeding shaft rotates at a uniform speed so that when the feed trough 61413 filled with salt particles faces downward, the salt particles in the feed trough 61413 fall into the discharge pipe 6144 and are discharged, so that the salt particles are quantitatively loaded into the sample tube 511.

[0039] Reference Figure 1 , Figure 4 and Fig.19 , then the translation module 65 drives the bottle body fixing module 66 fixed with the sample bottle to move to the bottom end of the homogenizing module 62, and the homogenizing module 62 adds homogenizers into the sample tube 511. Then, liquid is added into the sample tube 511 through the liquid adding module 63, and the liquid adding module 63 includes a liquid adding pump 631 and a liquid adding tube 632, wherein the liquid adding pump 631 is fixedly arranged at the bottom end of the rack 1, and the liquid adding tube 632 is arranged at the bottom end of the homogenizing module 62 and connected to the liquid adding pump 631. After the sample tube 511 moves to the bottom end of the liquid adding tube 632, the liquid adding pump 631 starts to work, thereby extracting the sample analysis liquid and adding it into the sample tube 511, thereby completing the liquid adding operation in the sample tube 511. Then the translation module 65 continues to drive the bottle body fixing module 66 fixed with the sample bottle to move to the bottom end of the switch cover device 64, and the switch cover device 64 re-covers the bottle cap on the sample tube 511 and tightens it.

[0040] After the sample tube 511 is covered with the bottle cap, the sample tube 511 is grabbed to the mixing area 7 by the three-axis manipulator 3, and the sample in the sample tube 511 is first vortexed and shaken by the vortex module 71. Then the three-axis manipulator 3 grabs the sample tube 511 to the vertical oscillation module 72 for vertical oscillation treatment, so that the sample in the sample tube is evenly mixed. After the sample in the sample tube 511 is vertically oscillated by the vertical oscillation module 72, the three-axis manipulator 3 grabs the sample tube 511 and places the sample tube 511 in the centrifuge 81, and the sample in the sample tube 511 is centrifuged and layered by the centrifuge 81, so that the upper clear liquid is formed in the sample tube 511. Then the three-axis manipulator 3 grabs the sample tube 511 and places it in the bottle body fixing module 66 for fixing, and the bottle cap on the sample tube 511 is opened again by the switch cover device 64. The XYZ moving module 2 drives the pipetting device 4 to move, and the disposable gun tip 521 on the gun tip rack 52 is sucked up by the pipetting device 4, and then the pipetting device 4 sucks up the upper layer of clear liquid in the sample tube 511 into the purification tube 512 through the disposable gun tip 521. Finally, the purification tube 512 can be grabbed by the three-axis manipulator 3 and placed in the refrigeration module 53 for refrigeration treatment.

[0041] The specific implementation principle of the embodiment of the present application is as follows: when the sample analysis pre-treatment instrument is needed to perform sample analysis pre-treatment, the XYZ moving module 2 drives the three-axis manipulator 3 to move, and the three-axis manipulator 3 grabs the sample tube 511 and places it on the bottle body placement table 661, and the bottle body clamping cylinder 662 clamps and fixes the sample tube 511. The vertical rodless cylinder 642 drives the bottle cap rotating module 645 and each clamping block 6452 to descend. When each clamping block 6452 descends to a suitable height, the rotating jaw 6451 drives each clamping block 6452 to clamp the bottle cap firmly, and then the driving unit 6453 drives the rotating jaw 6451 to rotate, thereby opening the bottle cap on the sample bottle.

[0042] The translation module 65 drives the sample bottle located on the bottle body fixing module 66 to move in the feeding area 6. When the sample bottle moves below the salt adding module 61, the salt particles in the storage barrel 613 are discharged from the discharge port 6131 to the feeding slot 61413 of the feeding shaft 6141 under the action of the stirring assembly 615. The rotating shaft of the driving motor 6121 rotates, and the rotating shaft 6122 is driven to rotate through the driving wheel 6124, the driven wheel 6125, the synchronous belt 6126 and the mounting frame 6127; during the rotation process, the rotating shaft 6122 drives the feeding shaft 6141 to rotate through the meshing driving gear 6123 and the driven gear 6142. The uniform rotation of the feed shaft 6141 causes the feed trough 61413 filled with salt particles to move vertically downward, and the salt particles in the feed trough 61413 fall into the discharge pipe 6144 under the action of gravity, and enter the sample bottle after passing through the discharge pipe 6144, thereby completing the quantitative addition of salt particles into the sample bottle. Subsequently, driven by the translation module 65, it moves to the bottom of the homogenizer module 62 to add homogenizers to the sample tube 511. Driven by the translation module 65, the sample tube 511 moves to the liquid adding module 63, and under the action of the liquid adding pump 631, the sample liquid flows into the sample tube 511 along the liquid adding pipe 632. Finally, the bottle cap is covered on the top of the sample tube 511 again through the switch cover device 64 and tightened.

[0043] The three-axis manipulator 3 grabs the sample bottle with the added sample to the vortex module 71, and vortexes and shakes the sample in the sample bottle through the vortex module 71. The three-axis manipulator 3 grabs the sample bottle to the vertical oscillation module 72, and further shakes the sample in the sample tube 511. The three-axis manipulator 3 grabs the sample tube 511 to the centrifuge 81 for centrifugation, so that the sample in the sample tube 511 is stratified. Then the sample tube 511 is grabbed again to the bottle body fixing module 66, and the bottle cap on the sample tube 511 is opened by the switch cover device 64.

[0044] The XYZ moving module 2 drives the liquid transfer device 4 to move, and the liquid transfer device 4 sucks up the disposable gun tip 521 to extract the upper layer of clear liquid in the sample tube 511 into the purification tube 512 through the disposable gun tip 521. Finally, the three-axis manipulator 3 grabs the purification tube 512 containing the upper layer of clear liquid to the refrigeration module 53 for refrigeration treatment, thereby completing the sample pre-analysis treatment.

[0045] The present invention is described above by way of example in conjunction with the accompanying drawings. It is obvious that the specific implementation of the present invention is not limited to the above-mentioned method. As long as various non-substantial improvements are made using the method concept and technical solution of the present invention, or the concept and technical solution of the present invention are directly applied to other occasions without improvement, they are all protected by the present invention.

Claims

1. An instrument for sample pre-analysis treatment, characterized in that: It includes a frame, an XYZ moving module, a three-axis manipulator and a liquid transfer device, wherein the XYZ moving module drives the three-axis manipulator and the liquid transfer device to perform XYZ three-dimensional movement; The rack is provided with a placement area, a feeding area, a mixing area and a centrifugal area, wherein the placement area is located at one end corner of the rack, and the placement area is provided with a refrigeration module, a centrifugal tube rack for placing sample tubes and purification tubes, and a gun tip rack for placing disposable gun tips; The centrifugal area is located on one side of the frame, and a centrifuge is provided at the centrifugal area; The feeding area is located between the placement area and the centrifugal area. The feeding area is provided with a salt adding module, a homogenizing module, a liquid adding module, a cover switch device and a translation module along its length direction. The translation module is provided with a bottle body fixing module for fixing the sample tube; The mixing zone is located at the front end of the placement zone, and the mixing zone is provided with a vortex module and a vertical oscillation module along its length direction; The salt adding module includes a base, a driving assembly, a storage barrel and a feeding assembly. A discharge port is formed at the bottom end of the storage barrel. The feeding assembly includes a feeding shaft, a fixing frame and a discharge pipe. The feeding shaft is horizontally and laterally rotatably arranged in the fixing frame. The feeding shaft includes a rod body and a bearing portion. A plurality of feed grooves are formed on the surface of the bearing portion. The bearing portion is located at the bottom end of the discharge port, and the discharge pipe is arranged at the bottom end of the bearing portion.

2. The instrument for sample pre-analysis processing according to claim 1, characterized in that: The driving assembly includes a driving motor, a rotating shaft and a driving gear. The rotating shaft is rotatably installed on the base and the storage barrel. The driving motor drives the rotating shaft to rotate. The driving gear is fixedly arranged on the rotating shaft. A driven gear meshing with the driving gear is fixedly arranged on the rod body.

3. The instrument for sample pre-analysis treatment according to claim 2, characterized in that: The driving assembly also includes a driving wheel, a driven wheel, a synchronous belt and a mounting frame, wherein the driving wheel is fixedly arranged on the rotating shaft of the driving motor, the mounting frame is fixedly arranged on the rotating shaft, the driven wheel is fixedly arranged on the periphery of the mounting frame, and the synchronous belt is wound around the driving wheel and the driven wheel.

4. The instrument for sample pre-analysis processing according to claim 2, characterized in that: One end of the rotating shaft is inserted into the material storage barrel, and a stirring assembly is arranged on the rotating shaft. The stirring assembly includes a stirring handle and a plurality of stirring blades. The stirring handle is relatively fixed to the rotating shaft, and the plurality of stirring blades are fixed on the peripheral side of the stirring handle. The rotating shaft rotates to drive the stirring handle and the stirring blades to rotate.

5. The instrument for sample pre-analysis processing according to claim 4, characterized in that: The stirring assembly is provided with four stirring blades, and two adjacent stirring blades are perpendicular to each other. The four stirring blades are fixed to each other and enclose a through groove. The bottom end of the stirring handle is provided with a protrusion, which is embedded in the through groove, and the peripheral side of the protrusion is in contact with the inner wall of the through groove.

6. The instrument for sample pre-analysis treatment according to claim 4, characterized in that: The stirring blade abuts against the bottom surface in the material storage barrel, and the blade surface of the stirring blade faces downward.

7. The instrument for sample pre-analysis processing according to claim 1, characterized in that: The XYZ moving module includes an X-axis component, a Y-axis component and a Z-axis component. The Z-axis component includes a first lifting motor and a second lifting motor. The first lifting motor drives the three-axis manipulator to perform lifting motion, and the second lifting motor drives the pipetting device to perform lifting motion.

8. The instrument for sample pre-analysis processing according to claim 1, characterized in that: The bottle body fixing module comprises a bottle body placing platform and a bottle body clamping cylinder, and the bottle body clamping cylinder is located above the bottle body placing platform.

9. The instrument for sample pre-analysis processing according to claim 1, characterized in that: The cover opening and closing device comprises a frame plate, a vertical rodless cylinder, a mounting plate, a plurality of vertical rodless cylinders and a bottle cap rotating module, wherein the vertical rodless cylinder is fixedly arranged on the frame plate and drives the mounting plate to rise and fall, and the plurality of vertical rodless cylinders are vertically fixedly arranged on the mounting plate and drive the bottle cap rotating module to rise and fall; The vertical rodless cylinder comprises a cylinder barrel, a slide, an upper end cover and a lower end cover, the outer sleeve of the cylinder barrel is provided with a pre-compression spring, the pre-compression spring is located between the slide and the upper end cover, and the bottle cap rotating module is fixed to the surface of the slide; The bottle cap rotating module includes a rotating jaw, a plurality of clamping blocks and a driving unit for driving the rotating jaw to rotate. The plurality of clamping blocks are fixedly arranged at the bottom end of the jaws of each rotating jaw. The clamping blocks are in a plurality of stepped shapes. The inner wall surface of the clamping blocks is formed into a plurality of layers of arcuate surfaces. The diameter of the arcuate surface of the inner wall of the clamping block decreases step by step toward the rotating jaw.

10. The instrument for sample pre-analysis processing according to claim 9, characterized in that: The clamp block includes a first clamping portion, a second clamping portion and a fixing portion, the first clamping portion is located at the upper end of the second clamping portion, and the diameter of the arc surface of the inner side wall of the first clamping portion is smaller than the diameter of the arc surface of the inner side wall of the second clamping portion.

11. The instrument for sample pre-analysis processing according to claim 10, characterized in that: The fixing portion is located outside the first clamping portion, and a transition block is provided between each clamping block and the jaws of the rotating clamping jaw, and two ends of the transition block are respectively fixedly connected to the fixing portion and the jaws of the rotating clamping jaw.

12. The instrument for sample pre-analysis processing according to claim 9, characterized in that: The outer sleeve of the cylinder is provided with a buffer spring, and the buffer spring is located between the slide table and the lower end cover.

13. The instrument for sample pre-analysis processing according to claim 9, characterized in that: The bottle cap rotating module also includes a fixing plate, which is respectively fixed to the slide table and the rotating clamp, a sliding seat is arranged on the fixing plate, a sliding guide rail is vertically arranged on the mounting plate, and the sliding seat is clamped on the sliding guide rail.

14. The instrument for sample pre-analysis treatment according to claim 1, characterized in that: The liquid adding module comprises a liquid adding pipe and a liquid adding pump. The liquid adding pump is arranged at the bottom end of the frame. The liquid adding pipe is located at the bottom end of the homogenizing submodule and is connected with the liquid adding pump.

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