Traditional Chinese medicine composition for treating psoriasis vulgaris as well as preparation method and application thereof

Abstract

The invention provides a traditional Chinese medicine composition for treating psoriasis vulgaris. The traditional Chinese medicine composition is prepared from the following raw material medicines in parts by weight: 2-5 parts of purslane, 2-4 parts of rhizoma smilacis glabrae, 1-3 parts of liquorice, 2-4 parts of humifuse euphorbia herb, 2-4 parts of muskmelon seed, 1-3 parts of folium artemisiae argyi, 1-3 parts of rhizoma nardostachyos and 1-3 parts of hollyhock seed. The invention further provides a preparation method and application of the traditional Chinese medicine composition. The traditional Chinese medicine composition disclosed by the invention is scientific in compatibility, is used for treating psoriasis vulgaris (static period), is remarkable in curative effect and simple in efficacy mechanism, and provides a new means for improving and treating psoriasis.

Description

Technical Field

[0001] The invention relates to a traditional Chinese medicine composition for treating psoriasis vulgaris, belonging to the field of traditional Chinese medicine. Background Art

[0002] Psoriasis is a chronic, recurrent and inflammatory skin disease induced by multiple factors. Its typical clinical manifestations are scaly erythema or plaques, and it can cause multiple complications such as metabolic syndrome, cardiovascular disease and inflammatory arthritis, which seriously affect the quality of life of patients. Epidemiological surveys show that the global prevalence is about 2% to 3%. Among all age groups, the severity of psoriasis in female patients is lower than that in males. The patient's condition worsens in autumn and winter. With the increase of social pressure, the accelerated pace of life and bad eating habits, it has shown an increasing trend year by year in recent years. Psoriasis not only affects the patient's appearance, but also has a long course and is prone to recurrence, causing serious physical and mental distress to patients, leading to heavy economic and social burdens.

[0003] At present, topical drugs for psoriasis include emollients, biological agents, vitamin D3 derivatives, retinoic acid, glucocorticoids, calcineurin inhibitors, AhR agonists and tar preparations. Traditional systemic treatment drugs mainly include retinoic acid, methotrexate and cyclosporine. Most oral drugs have serious side effects, and long-term use affects liver and kidney function. Relapse or even rebound is easy after discontinuation of medication, making it difficult to maintain efficacy. A variety of new biological agents require continuous injection, are expensive, and have a reducing effect on human immunity. Therefore, the demand for safe, comfortable and effective treatment of psoriasis is becoming more and more urgent.

[0004] The TCM name of psoriasis is "white sputum". Doctors of all dynasties believed that its essence lies in "blood", so they treated it according to the "blood division". Whether it is exogenous wind, cold, dampness, poison, or internal pathological factors such as dryness and stasis, they can all fight with the blood and make the blood vessels hot, which is a blood heat syndrome; or excessive heat damages the body fluid, which is a blood dryness syndrome; or long-term disease causes blood stasis, which is a blood stasis syndrome. Therefore, the three main symptoms of psoriasis are formed in the "blood division" treatment. In the early stage of this disease, it is mostly blood heat. If the heat in the blood cannot be removed in time, it will consume the yin blood for a long time, which is easy to cause yin blood deficiency, wind and dryness, and skin malnutrition, which is a blood dryness syndrome; or toxic heat scorches the yin blood, and the qi and blood flow is not smooth, which is a blood stasis syndrome. Therefore, blood heat syndrome is often seen in the early and progressive stages. Symptoms include new skin lesions, bright red color, covered with silvery white scales, many scales and easy to fall off, accompanied by skin itching, irritability, dry mouth, constipation, and yellow and red urine. The tongue is red with thin yellow fur, and the pulse is rapid or stringy or slippery. The treatment is mainly to dispel wind and heat, cool blood and detoxify.

[0005] In the treatment of psoriasis with external treatment of traditional Chinese medicine, the drugs can act directly on the surface of the skin, the drug power reaches the lesion directly, and the curative effect is remarkable. Chinese medicine extracts are a class of substances with a certain odor extracted from Chinese medicinal plants. The ingredients are natural and can act directly on the surface of the skin. They are safe and have no side effects. The active ingredients in Chinese medicine can directly increase blood circulation, promote the discharge of toxins on the body surface, and quickly promote the shedding of dandruff; at the same time, they can reduce epidermal inflammation. The nutrients in the medicine can also moisturize the skin, reduce the production of scales, and increase skin elasticity. Chinese medicine bath therapy is a treatment method with distinctive characteristics, and it is also the main treatment method for clinical psoriasis vulgaris. It is expected to become a treatment method with good family treatment and compliance, economy, safety, and few side effects.

[0006] Application number: CN202210920145.8, invention name: A Chinese medicine composition and lotion for treating psoriasis, providing a Chinese medicine composition and lotion for treating psoriasis, belonging to the technical field of Chinese medicine. The Chinese medicine composition provided by the invention uses purslane and phellodendron as the main medicines, and is compounded with herba rutinae, cortex daphne, fructus cnidii, fructus salviae miltiorrhizae, poria cocos, and licorice. Aiming at the characteristics of spleen deficiency and dampness excess syndrome of plaque psoriasis, the combination of various medicines can play the role of strengthening the spleen and removing dampness, detoxifying and relieving itching, and has a significant effect on psoriasis vulgaris. Summary of the invention

[0007] The present invention provides a Chinese medicine composition for treating psoriasis vulgaris, mainly for treating psoriasis vulgaris in the quiescent stage. The present invention also provides a preparation method and use of the Chinese medicine composition.

[0008] The present invention provides a traditional Chinese medicine composition for treating psoriasis vulgaris, which is prepared from the following raw materials in weight proportion:

[0009] 2-5 parts of purslane, 2-4 parts of wild yam, 1-3 parts of licorice, 2-4 parts of ground ivy, 2-4 parts of melon seeds, 1-3 parts of mugwort, 1-3 parts of nard, and 1-3 parts of hibiscus.

[0010] Preferably, it is prepared from the following bulk drugs in the following weight ratio:

[0011] 5 parts of purslane, 3 parts of smilax glabra, 2 parts of liquorice, 3 parts of herba euphorbiae, 3 parts of musk melon seeds, 2 parts of wormwood leaves, 2 parts of nard, and 3 parts of marshmallow seeds.

[0012] The Chinese medicine composition of the present invention is prepared from the raw powder of the raw material medicine, water or organic solvent extract as active ingredients, and pharmaceutically acceptable excipients or auxiliary ingredients to form a commonly used external preparation in medicine.

[0013] It is prepared from crude powder of the original drug, separating oil and water after steam distillation, filtering the decoction, concentrating, mixing with oil, freeze-drying, and then adding pharmaceutically acceptable excipients or auxiliary ingredients to prepare a commonly used external preparation in medicine;

[0014] Alternatively, coarse powders of Herba Euphorbiae Immaturus, Folium Artemisiae Argyi and Nardostachys grandiflora are subjected to steam distillation, and aromatic water, distillation mother liquor and medicinal residues are collected for later use; five medicinal materials of Portulaca oleracea, Smilax glabra, Licorice, Melon seeds and Althaea officinalis seeds and the medicinal residues are mixed, extracted with water, filtered, the distillation mother liquors are combined, concentrated, impurities are removed, aromatic water is added, the pH value is adjusted, water is added, mixed, and then pharmaceutically acceptable excipients or auxiliary ingredients are added to prepare a commonly used external preparation in medicine.

[0015] Wherein, the external preparation is lotion, tincture, spray, paint, ointment, cream, gel, paste, powder, patch, film coating.

[0016] The present invention provides a method for preparing the Chinese medicine composition for treating psoriasis vulgaris, which comprises the following steps:

[0017] a. Weigh the APIs of various weight ratios;

[0018] b. Crush the medicinal materials into coarse powder, mix them evenly, put them in a volatile oil extraction tank, steam distill them, let them stand until the oil and water are separated in the oil-water separator, filter the decoction, concentrate it, mix it with oil, and prepare it into freeze-dried powder.

[0019] Or, comprising the steps of:

[0020] a. Weigh the APIs of various weight ratios;

[0021] b. Grind the herb of wormwood, wormwood leaf and nard into coarse powder, mix them evenly, add steam for distillation extraction, collect the aromatic water, distillation mother liquor and medicinal residues respectively, and set them aside; add polysorbate 80 to the aromatic water, mix them evenly, and set them aside; the extraction is to add 5-10 times the amount of water, and distill and extract for 3-6 hours;

[0022] c. Take five medicinal materials of purslane, smilax glabra, licorice, melon seeds, marshmallow seeds and the Chinese medicine residue in step b, add water to extract, filter, combine the distillation mother liquor in step b, concentrate, remove impurities, and set aside; add aromatic water to the medicinal liquid after impurities removal in step c, adjust the pH value to 4.0-6.0, add water, mix, and package to obtain;

[0023] The extraction is performed by decoction for three times, adding 6 times the amount of water each time, and decoction for 40 minutes; the reduced pressure concentration condition is 50-70° C., and the relative density is concentrated to 1.05-1.15 g / ml;

[0024] The impurity removal comprises adding ethanol to an alcohol content of 50-70%, refrigerating for 12-36 hours, and concentrating.

[0025] The present invention provides use of the traditional Chinese medicine composition in preparing a medicine for treating psoriasis vulgaris.

[0026] Preferably, the drug is a drug for treating psoriasis vulgaris in the quiescent phase; the drug is used to alleviate symptoms of scaly erythema and plaques.

[0027] Preferably, the drug is a drug for treating blood-heat and dampness type psoriasis;

[0028] The invention provides use of the traditional Chinese medicine composition in preparing antibacterial and anti-inflammatory drugs.

[0029] TCM's understanding of the treatment of psoriasis includes "treating from the lungs", "arguing from the blood", "arguing from the deficiency", etc. The mainstream view is "treating from the blood", believing that psoriasis is caused by the deficiency of blood, wind and dryness, and skin malnutrition. "Blood heat" is the root cause and key to the onset of psoriasis. In the drug prescription of the present invention, purslane and herba euphorbiae are cold in nature, have the effects of clearing heat and detoxifying, and cooling blood, and play the effects of clearing heat and detoxifying, and cooling blood together as the main medicine; hibiscus seeds and wild smilax glabra clear heat and detoxify and eliminate dampness, and are the minister medicines; wormwood leaves and sweet pinellia are warm in nature, and both have the effects of removing dampness and relieving itching, and are used as cold-controlling drugs; licorice and melon seeds harmonize the various drugs and moisturize and detoxify, and are the guiding drugs. The combination of various drugs can achieve the effects of clearing heat and cooling blood, removing dampness and relieving itching.

[0030] The Chinese medicine external-use composition of the present invention has a scientific compatibility and conforms to the theory of traditional Chinese medicine. The components work synergistically and have the effects of clearing away heat and detoxifying, cooling blood, removing dampness and relieving itching, and moisturizing the skin. It is used for psoriasis with more scales and red rashes, has a significant therapeutic effect, and the efficacy mechanism is simple, which provides a new choice for the improvement and treatment of psoriasis. DETAILED DESCRIPTION

[0031] Example 1 Preparation of the Chinese medicine composition of the present invention

[0032] Take 20g of purslane, 20g of wild yam, 10g of licorice, 20g of ground ivy, 30g of melon seeds, 20g of mugwort, 20g of nard, and 10g of marshmellow seed.

[0033] The Chinese medicinal composition is extracted by steam distillation and water decoction respectively.

[0034] Process (1): The above medicinal materials are crushed into coarse powder, mixed evenly, placed in a volatile oil extraction tank, and steam distilled for 5 hours. The mixture is allowed to stand for a period of time until the oil and water are separated in an oil-water separator. The decoction is filtered, concentrated, and prepared into freeze-dried powder.

[0035] Process (2): Grind Herba Lysimachiae, Artemisiae Argyi and Nardostachys grandiflora into coarse powder, mix them evenly, add water to steam for distillation extraction, collect aromatic water, distillation mother liquor and medicinal residues respectively, and set them aside; add polysorbate 80 to the aromatic water, mix them evenly, and set them aside; take five medicinal materials of Portulaca oleracea, Smilax glabra, Licorice, Melon seeds and Althaea officinalis and the medicinal residues in step b, extract them with water, filter, combine the distillation mother liquor in step b, concentrate, remove impurities, and set them aside; add the aromatic water to the medicinal solution after impurities are removed in step c, adjust the pH value, add water, mix, and package to obtain the product.

[0036] The extract of process 1 (traditional Chinese medicine composition and freeze-dried powder) and the extract of process 2 (traditional Chinese medicine composition) were respectively prepared into external lotions, each 100 ml lotion containing 30 g of raw medicinal materials.

[0037] The extract of process 1 (traditional Chinese medicine composition and freeze-dried powder) and the extract of process 2 (traditional Chinese medicine composition) were dissolved in water containing 1.00% Tween-80 and ultrasonically emulsified, and each 100 ml contained 45 g of the raw medicinal material.

[0038] The beneficial effects of the present invention are demonstrated by pharmacodynamic tests as follows.

[0039] Test Example 1 Preliminary clinical trial of the drug of the present invention in the treatment of psoriasis vulgaris (quiescent stage)

[0040] 20 volunteers with psoriasis vulgaris (quiescent stage) were divided into 2 groups, and used process 1 extract lotion and process 2 extract lotion for bathing once every 2 days for 3 consecutive months. Questionnaire survey was conducted before and after use, and skin care was performed according to previous habits during use. The questionnaire survey results showed that the subjects self-evaluated that process 2 extract lotion was better than process 1 in improving psoriasis symptoms.

[0041] Experimental Example 2 Animal Experiment on the Treatment of Psoriasis Vulgaris with the Drug of the Present Invention

[0042] A 5% imiquimod cream was used to replicate a psoriasis vulgaris mouse model. The experimental groups were treated with process 1 extract lotion and process 2 extract lotion, respectively, evenly applied to the depilated area, 1 ml twice a day, for 30 consecutive days; the model group was treated with an equal amount of 1.00% Tween water, and the effects of the lotions on the psoriasis symptoms of mice were evaluated by the scores of mouse skin erythema and scaling. The scores of skin erythema and scaling in the process 2 extract group were significantly lower than those in the process 1 extract group, and the process 2 extract had a better improvement effect on psoriasis symptoms than the process 1 extract.

[0043] The experimental results are shown in Table 1.

[0044] Table 1 Effects of different extraction methods on the improvement of psoriasis symptoms

[0045] Group n Erythema score Scaly score Model Group 8 7.46±0.33 26.27±1.16 Process 1 Extract Group 8 6.64±0.38** 24.88±1.01** Process 2 Extract Group 8 5.80±0.45** 22.32±1.06***

[0046] Compared with the model group, *P<0.05, **P<0.01; compared with the process 1 extract group, ▲P<0.05, ▲▲P<0.01

[0047] Test Example 3 Screening test of different raw material ratios of the Chinese medicine composition of the present invention

[0048] The following three formulations are compared:

[0049] (1) 2 parts of purslane, 2 parts of smilax glabra, 1 part of liquorice, 2 parts of herba euphorbiae, 3 parts of melon seeds, 2 parts of wormwood leaves, 2 parts of nard, and 1 part of marsh mallow seeds.

[0050] (2) 5 parts of purslane, 3 parts of smilax glabra, 2 parts of liquorice, 3 parts of herba euphorbiae, 3 parts of musk melon seeds, 2 parts of wormwood leaves, 2 parts of nard, and 3 parts of marshmallow seeds.

[0051] (3) 3 parts of purslane, 3 parts of smilax glabra, 2 parts of liquorice, 3 parts of herba euphorbiae, 5 parts of musk melon seeds, 3 parts of wormwood leaves, 3 parts of nard, and 2 parts of marshmellow seeds.

[0052] The above medicinal materials were weighed in proportion, extracted by the steam distillation method and water decoction method of Example 1, respectively, to prepare an external lotion.

[0053] A 5% imiquimod cream was used to replicate a mouse model of psoriasis vulgaris. The experimental group applied the lotion evenly to the depilatory area, 1 ml twice a day, for 30 consecutive days; the model group applied the same amount of basic lotion, and the effect of the lotion on the psoriasis symptoms of mice was evaluated by the mouse skin erythema and scaling scores. Prescription 2 had the strongest effect on improving the psoriasis symptoms of mice. The results are shown in Table 2.

[0054] Table 2 Effects of different prescription compatibility ratios on the improvement of psoriasis symptoms

[0055] Group n Erythema score Scaly score Model Group 8 7.51±0.33 26.50±1.04 Prescription 1 set 8 6.64±0.38** 24.88±1.01** Prescription 2 sets 8 5.80±0.45** 22.32±1.06*** Prescription 3 groups 8 6.12±0.33** 23.81±1.45***

[0056] Compared with the model group, *P<0.05, **P<0.01; compared with the prescription 1 group, ▲P<0.05, ▲▲P<0.01

[0057] Experimental Example 4 Effect of the Chinese medicine composition of the present invention on a mouse model of psoriasis vulgaris

[0058] A 5% imiquimod cream was used to replicate a psoriasis vulgaris mouse model. The erythema and scaling scores of the mouse skin were used to evaluate the effect of the lotion on the psoriasis symptoms in mice. The ELISA method was used to determine the levels of VEGF and TGF-β1 in the mouse skin to preliminarily explore the mechanism of the lotion in treating psoriasis.

[0059] 1. Establishment of Psoriasis Vulgaris Mouse Model

[0060] The hair on the back of the mouse (2 cm × 3 cm) was shaved, and the short hair was removed with a depilatory cream. 5% imiquimod cream (50 mg / cm) was applied to the depilatory area. 2 , once a day for 2 weeks. The skin on the back of the mouse was obviously thickened, reddish in color, and covered with thick scales. After the scales were removed, punctate bleeding was seen, indicating that the psoriasis model was successfully established.

[0061] 2. Experimental groups and drug administration methods

[0062] 84 SPF grade 5-7 week old male C57BL / 6 mice, weighing 20±2g, were housed in the laboratory for 3 days and randomly divided into a blank group, a model group, a lotion group 1 (concentration 100%), a lotion group 2 (diluted with a basic lotion to a lotion concentration of 50%), a lotion group 3 (diluted with a basic lotion to a lotion concentration of 10%), a lotion group 4 (diluted with a basic lotion to a lotion concentration of 5%), and a positive drug group (0.05g / ml glucocorticoid), with 12 mice in each group. The lotion group was prepared using the prescription ratio of prescription 2 in Test Example 3.

[0063] Except for the blank group, which only underwent hair removal, the mice in the model group and the experimental group were treated with the same method to establish the psoriasis vulgaris mouse model. After modeling, the mice in the model group and the experimental group continued to be treated with 5% imiquimod cream at 50 mg / cm 2 , once a day; the lotion group evenly applied 0.1 ml of lotion on the back hair removal experimental observation area, the model group applied the same volume of basic lotion, and the positive drug group applied the same volume of glucocorticoid, twice a day, for 30 consecutive days.

[0064] 3. Evaluation indicators

[0065] The skin condition of the back observation area of ​​each group of mice was observed every day, including the basic physiological characteristics of the skin and color changes, and the erythema and scaling scores of the mice skin were recorded. On the 15th and 30th days after administration, 10 skin sites were randomly selected in the experimental area, and the erythema and scaling scores were measured. The average value was calculated to evaluate the improvement of psoriasis symptoms.

[0066] At the end of the experiment, the mice were killed, and the skin of the experimental area was taken for tissue homogenization and centrifuged at 3000r / min for 10min. The supernatant was taken and the VEGF and TGF-β1 contents were determined by ELISA.

[0067] 4. Experimental results and conclusions

[0068] When the 100% concentration of the Chinese herbal lotion was applied, the mice showed no allergic reactions such as redness, swelling, or rash, indicating that the Chinese herbal lotion has little irritation to the skin.

[0069] The scores of skin erythema and scaling of mice in the model group and experimental group were significantly higher than those in the blank group. Compared with the model group, the scores of skin erythema and scaling of mice in the lotion group and positive drug group were significantly reduced (P<0.05). On the 15th day after administration, compared with the model group, the scores of skin erythema and scaling of mice in the lotion group 1 and group 2 were significantly reduced (P<0.05), and the scores of skin erythema and scaling of mice in the positive drug glucocorticoid group were also reduced, but there was no significant difference. On the 30th day after administration, compared with the model group, the scores of skin erythema and scaling of mice in the lotion group and positive drug group were significantly reduced. The skin erythema and scaling scores were significantly reduced (P<0.05); compared with the blank group, the model group had a significantly reduced VEGF content in the mouse skin, and a significantly increased TGF-β1, with statistically significant differences (P<0.01). After 30 days of administration, the lotion group and the positive drug group had a significantly increased VEGF content in the mouse skin, and a significantly decreased TGF-β1 (P<0.05) compared with the model group; compared with the positive drug group, lotion group 1, lotion group 2, and lotion group 3 were better than the positive drug group, and lotion group 4 was equivalent to it. The results are shown in Table 3.

[0070] Table 3 Effects of lotion on skin erythema, scaling scores, skin VEGF and TGF-β1 levels in mice with psoriasis vulgaris

[0071]

[0072]

[0073] Compared with the model group, *P<0.05, **P<0.01; compared with the blank group, #P<0.05, ##P<0.01; compared with the positive drug group, ▲P<0.05, ▲▲P<0.01.

[0074] Growth factors VEGF and TGF-β1 participate in skin inflammatory responses by regulating cell proliferation, differentiation and apoptosis. Vascular endothelial growth factor VEGF promotes cell growth and capillary formation, thereby promoting skin repair. TGF-β1 binds to its receptor and activates the downstream caspase apoptosis signaling pathway, mediating the occurrence of cell apoptosis. In skin inflammation, it induces apoptosis of skin cells, thereby aggravating the inflammatory response. Therefore, inhibiting the secretion of TGF-β1 can achieve an anti-inflammatory effect.

[0075] In the experiment, the VEGF content in the skin of mice in the model group was significantly reduced, and TGF-β1 was significantly increased. The lotion group can produce anti-inflammatory and skin repair effects by increasing VEGF and reducing the expression of TGF-β1.

[0076] Experimental Example 5 Effect of the Chinese medicine composition of the present invention on patients with psoriasis vulgaris

[0077] Twenty volunteers with both genders, aged 25-45 years, who suffered from psoriasis vulgaris (quiescent stage) from June 2022 to September 2022 in a community in Chengdu, were selected.

[0078] 1. Selection criteria

[0079] ① Mild to moderate psoriasis, with skin lesions covering less than 10% of the body surface area;

[0080] ②No other diseases related to psoriasis in the recent period;

[0081] ③ Participate voluntarily and sign the informed consent.

[0082] 2. Exclusion criteria

[0083] ① The skin lesion area is greater than 10% of the body surface area;

[0084] ② Those who are allergic to Chinese medicine lotion;

[0085] ③ Those with severe systemic diseases, immunodeficiency or autoimmune diseases;

[0086] ④ Those who suffer from other dermatological diseases or are receiving medication in the test area;

[0087] ⑤ Have used topical or systemic psoriasis treatment products within three months before the start of the trial;

[0088] ⑥ Those who have received phototherapy or systemic treatment.

[0089] 3. Dosage

[0090] Add 42-44℃ warm water to the Chinese medicine lotion, pour it into a basin, and bathe the whole body for 20-30 minutes. Do it once every other day for 2 consecutive weeks as a course of treatment. Before using the lotion and 4 weeks after using the lotion, volunteers will undergo skin microscopic imaging tests on the lesion area, and at the same time, they will complete a questionnaire survey; a skin examination will be conducted in the test area midway to facilitate identification of the test area.

[0091] 4. Treatment or improvement of psoriasis vulgaris

[0092] After using the lotion for 4 weeks, the lesion area of ​​the volunteers increased from 10.35cm 2 Reduced to 8.72cm 2 , the scores of skin lesions erythema and scaling were significantly reduced (P < 0.05); in the questionnaire survey, the subjects' self-assessed psoriasis symptoms and skin conditions were significantly improved. 75% of the subjects believed that the lotion had a significant effect on improving erythema and scaling; 85% of the subjects believed that the lotion had a significant effect on relieving skin itching. The results are shown in Table 4.

[0093] Table 4 The therapeutic or improving effects of lotions on patients with psoriasis vulgaris

[0094] Group <![CDATA[Skin lesion area (cm 2 )]]> Erythema score Scaly score Before using lotion 10.35±1.88 7.44±0.33 26.26±1.16 After using lotion 8.72±1.33* 5.42±0.80* 22.05±1.29*

[0095] Compared with before using lotion, *P<0.05, **P<0.01.

[0096] Test Example 6: Verification test of the best prescription of the Chinese medicine of the present invention

[0097] In order to further verify the synergistic effect of the Chinese medicine composition of the present invention, a negative control group was set up, and a simplified prescription of Portulaca oleracea and Herba Euphorbiae was used for comparative tests. The negative control group is only for psoriasis of heat-toxicity type, with symptoms of bright red skin lesions, burning pain, red tongue with yellow fur, and rapid pulse, but its single heat-clearing and detoxifying mechanism cannot fully intervene in the pathological links of psoriasis. In contrast, the whole prescription composition of the present invention, through the synergy of multiple drugs, takes into account the effects of clearing heat and detoxifying, cooling blood, removing dampness and relieving itching, and moisturizing the skin, and is suitable for psoriasis of blood-heat and dampness type, and the therapeutic effect is significantly better than that of a single drug group.

[0098] The specific tests are as follows:

[0099] 1. Prescription for negative control group: 5 parts of Portulaca oleracea and 5 parts of Herba Euphorbiae.

[0100] 2. Preparation method:

[0101] 1) Weigh purslane and herba euphorbiae, grind into coarse powder, and mix well;

[0102] 2) Decoction and extract 3 times, adding 6 times the amount of water each time, and decoct for 40 minutes;

[0103] 3) The filtrates were combined and concentrated under reduced pressure to a relative density of 1.05-1.15 g / ml;

[0104] 4) Add ethanol to 60% alcohol content, refrigerate for 24 hours, and filter to remove impurities;

[0105] 5) After concentration, add water to 1000 ml, adjust the pH to 5.0, and dispense.

[0106] 6. Experimental methods:

[0107] A 5% imiquimod cream was used to replicate a mouse model of psoriasis vulgaris. The experimental group was treated with the whole prescription (prescription in Example 2) and the negative control group lotion, which was evenly applied to the depilatory area. Both groups were applied twice a day, 1 ml each time, for 30 consecutive days. The effect of the lotion on the symptoms of psoriasis in mice was evaluated by scoring the erythema and scaling of the mouse skin, and the contents of VEGF and TGF-β1 in the skin were determined.

[0108] 7. Experimental results:

[0109] Group Erythema score Scaly score VEGF (pg / ml) TGF-β1(pg / ml) Model Group 7.55±0.96 26.79±1.31 5.42±0.80 169.01±15.32 All-round group 4.64±0.78 20.17±1.32 13.94±1.16 91.22±12.30 Negative control group 6.12±0.85 24.58±1.28 8.35±0.92 142.16±14.75

[0110] 8. Conclusion:

[0111] The efficacy of the negative control group was significantly lower than that of the whole prescription group (P<0.01), indicating that the original combination enhanced the overall efficacy through multi-drug synergy;

[0112] The negative control group had a weaker regulatory effect on VEGF and TGF-β1, confirming that its single heat-clearing and detoxifying mechanism cannot fully intervene in the pathological process of psoriasis.

[0113] The Chinese medicine lotion of the present invention has a scientific compatibility and is used for treating psoriasis vulgaris (quiescent stage). It has a significant therapeutic effect and a simple efficacy mechanism, and provides a new choice for the improvement and treatment of psoriasis.

Claims

1. A Chinese medicine composition for treating psoriasis vulgaris, characterized in that: It is prepared from the following raw materials in the following weight ratio: 2-5 parts of purslane, 2-4 parts of wild yam, 1-3 parts of licorice, 2-4 parts of ground ivy, 2-4 parts of melon seeds, 1-3 parts of mugwort, 1-3 parts of nard, and 1-3 parts of hibiscus.

2. The Chinese medicine composition for treating psoriasis vulgaris according to claim 1, characterized in that: It is prepared from the following raw materials in the following weight ratio: 5 parts of purslane, 3 parts of smilax glabra, 2 parts of liquorice, 3 parts of herba euphorbiae, 3 parts of musk melon seeds, 2 parts of wormwood, 2 parts of nard, and 3 parts of marshmallow seeds.

3. The Chinese medicine composition for treating psoriasis vulgaris according to claim 1 or 2, characterized in that: It is prepared from the raw powder of the raw material medicine, water or organic solvent extract as active ingredients, and pharmaceutically acceptable excipients or auxiliary ingredients to form a commonly used external preparation in medicine.

4. The Chinese medicine composition for treating psoriasis vulgaris according to claim 3, characterized in that: It is prepared from crude powder of the original drug, separating oil and water after steam distillation, filtering the decoction, concentrating, mixing with oil, freeze-drying, and then adding pharmaceutically acceptable excipients or auxiliary ingredients to prepare a commonly used external preparation in medicine; Alternatively, coarse powders of Herba Euphorbiae Immaturus, Folium Artemisiae Argyi and Nardostachys grandiflora are subjected to steam distillation, and aromatic water, distillation mother liquor and medicinal residues are collected for later use; five medicinal materials of Portulaca oleracea, Smilax glabra, Licorice, Melon seeds and Althaea officinalis seeds and the medicinal residues are mixed, extracted with water, filtered, the distillation mother liquors are combined, concentrated, impurities are removed, aromatic water is added, the pH value is adjusted, water is added, mixed, and then pharmaceutically acceptable excipients or auxiliary ingredients are added to prepare a commonly used external preparation in medicine.

5. The Chinese medicine composition for treating psoriasis vulgaris according to claim 3 or 4, characterized in that: The external preparation is a lotion, a tincture, a spray, a paint, an ointment, a cream, a gel, a paste, a powder, a patch, and a film-coating.

6. A method for preparing the Chinese medicine composition for treating psoriasis vulgaris according to any one of claims 1 to 5, characterized in that: It includes the following steps: a. Weigh the APIs of various weight ratios; b. Crush the medicinal materials into coarse powder, mix them evenly, put them in a volatile oil extraction tank, steam distill them, let them stand until the oil and water are separated in the oil-water separator, filter the decoction, concentrate it, mix it with oil, and prepare it into freeze-dried powder. Or, comprising the steps of: a. Weigh the APIs of various weight ratios; b. Grind the herb of wormwood, wormwood leaf and nard into coarse powder, mix them evenly, add steam for distillation extraction, collect the aromatic water, distillation mother liquor and medicinal residues respectively, and set them aside; add polysorbate 80 to the aromatic water, mix them evenly, and set them aside; the extraction is to add 5-10 times the amount of water, and distill and extract for 3-6 hours; c. Take five medicinal materials of purslane, smilax glabra, licorice, melon seeds, marshmallow seeds and the Chinese medicine residue in step b, add water to extract, filter, combine the distillation mother liquor in step b, concentrate, remove impurities, and set aside; add aromatic water to the medicinal liquid after impurities removal in step c, adjust the pH value to 4.0-6.0, add water, mix, and package to obtain; The extraction is performed by decoction for three times, adding 6 times the amount of water each time, and decoction for 40 minutes; the reduced pressure concentration condition is 50-70° C., and the relative density is concentrated to 1.05-1.15 g / ml; The impurity removal comprises adding ethanol to an alcohol content of 50-70%, refrigerating for 12-36 hours, and concentrating.

7. Use of the Chinese medicine composition according to any one of claims 1 to 5 in the preparation of a medicament for treating psoriasis vulgaris.

8. The use according to claim 7, characterized in that: The medicine is a medicine for treating the dormant period of common psoriasis; the medicine is used to alleviate the symptoms of scaly erythema and plaques.

9. The use according to claim 7, characterized in that: The medicine is a medicine for treating blood-heat and dampness type psoriasis.

10. Use of the Chinese medicine composition according to any one of claims 1 to 5 in the preparation of antibacterial and anti-inflammatory drugs.

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