A method for enhancing nitrogen removal in an anaerobic ammonium oxidation system through the synergy of tea polyphenols and ferric ions

Abstract

The present invention relates to the technical field of wastewater treatment, and discloses a method for synergistically enhancing nitrogen removal in an anaerobic ammonium oxidation system based on tea polyphenols and ferric iron. The method includes: inoculating anaerobic ammonium oxidation sludge and adding wastewater into an anaerobic ammonium oxidation reactor, and then adding tea polyphenol-Fe<supgt;3+< / supgt; to treat the wastewater. In the present invention, tea polyphenols can promote the absorption of ferric iron by anaerobic ammonium oxidation bacteria, enhancing the biological utilization of iron elements in the anaerobic ammonium oxidation system. The iron elements absorbed by anaerobic ammonium oxidation bacteria can be used for synthesizing key functional enzymes, enhancing the anaerobic ammonium oxidation and dissimilatory nitrate reduction to ammonium metabolic activities of anaerobic ammonium oxidation bacteria, and at the same time promoting the coupling of the two nitrogen conversion pathways, synergistically improving the total nitrogen removal rate and enhancing the nitrogen removal efficiency of the anaerobic ammonium oxidation system.

Description

Technical Field

[0001] The present invention relates to the technical field of wastewater treatment, and particularly relates to a method for synergistically enhancing nitrogen removal in an anaerobic ammonia oxidation system based on tea polyphenols and ferric iron. Background Art

[0002] The anaerobic ammonia oxidation (Anammox) process is a sustainable sewage biological treatment technology, which has the advantages of low aeration energy consumption, low sludge yield, and no need for external carbon source, and has good application potential in the treatment of high ammonia nitrogen wastewater such as landfill leachate, sludge digestate, and coking wastewater.

[0003] The principle of the anaerobic ammonia oxidation process is that anaerobic ammonia oxidizing bacteria convert ammonia nitrogen (NH4 + −N) and nitrite nitrogen (NO2 − −N) into nitrogen gas, and at the same time produce a small amount of nitrate nitrogen (NO3 − −N).

[0004] However, in practical engineering applications, anaerobic ammonia oxidizing bacteria grow slowly and are sensitive to environmental conditions, resulting in problems such as limited nitrogen removal efficiency of the system. In addition, 11% of nitrate nitrogen is produced during the anaerobic ammonia oxidation process, which further limits the total nitrogen removal rate (NRE) of the system. Although some anaerobic ammonia oxidizing bacteria (such as Candidatus Brocadia and Candidatus Jettenia) can reduce nitrate nitrogen to nitrite nitrogen and ammonia nitrogen by the dissimilatory nitrate reduction to ammonium (DNRA) pathway, using small molecular organic matter as an electron donor, their DNRA activity is low, and it is difficult to effectively consume the nitrate nitrogen produced during the anaerobic ammonia oxidation process.

[0005] As an important trace element for microbial growth and metabolism, iron participates in the formation of Anammox functional enzymes such as hydrazine synthase (HZS) and hydrazine dehydrogenase (HDH), as well as DNRA functional enzymes such as periplasmic dissimilatory nitrate reductase (NAP), cytoplasmic NADH-dependent nitrite reductase (NIR), and periplasmic dissimilatory nitrite reductase (NRF), which helps to improve the Anammox and DNRA metabolic activities of anaerobic ammonia oxidizing bacteria.

[0006] However, in the anaerobic ammonia oxidation system (pH 7.0 - 8.5, redox potential -100 - 400 mV), iron mainly exists in the form of ferric iron, while anaerobic ammonia oxidizing bacteria can only directly absorb dissolved divalent iron, which limits the application of iron in enhancing the anaerobic ammonia oxidation process.

[0007] Tea polyphenols (TP) are important cytokines for microorganisms to uptake iron in natural water bodies, and can stably chelate dissolved ferric iron (Fe 3+). Introducing tea polyphenols into the anaerobic ammonium oxidation system is expected to promote the absorption of ferric iron by anaerobic ammonium-oxidizing bacteria, thereby enhancing their Anammox and DNRA metabolic activities. Moreover, through the coupling of the Anammox and DNRA nitrogen transformation pathways, the total nitrogen removal rate can be synergistically improved, and the nitrogen removal efficiency of the anaerobic ammonium oxidation system can be strengthened. Summary of the Invention

[0008] The object of the present invention is to provide a method for synergistically strengthening the nitrogen removal of an anaerobic ammonium oxidation system based on tea polyphenols and ferric iron, aiming at the problem that the nitrogen removal activity of anaerobic ammonium-oxidizing bacteria is low during the actual application process, resulting in limited total nitrogen removal rate of the anaerobic ammonium oxidation system.

[0009] To achieve the above object, the first aspect of the present invention provides a method for synergistically strengthening the nitrogen removal of an anaerobic ammonium oxidation system based on tea polyphenols and ferric iron, and the method includes:

[0010] In an anaerobic ammonium oxidation reactor, inoculate anaerobic ammonium oxidation sludge, add wastewater, and then add tea polyphenol-Fe 3+ to treat the wastewater.

[0011] The method provided by the present invention at least further has the following beneficial effects:

[0012] (1) By externally adding tea polyphenols, the present invention promotes the absorption of ferric iron by anaerobic ammonium-oxidizing bacteria, can effectively enhance the biological utilization of iron elements, and improve the intracellular iron element content of anaerobic ammonium-oxidizing bacteria.

[0013] (2) The present invention promotes the synthesis of Anammox and DNRA functional enzymes with the help of tea polyphenol-Fe 3+ , which is beneficial to enhancing the Anammox and DNRA metabolisms of anaerobic ammonium-oxidizing bacteria and strengthening the nitrogen removal efficiency of the anaerobic ammonium oxidation system.

[0014] (3) The tea polyphenols adopted by the present invention are inexpensive and have a low dosage, with good cost-effectiveness.

[0015] (4) The method of the present invention is simple to operate, environmentally friendly, economical and efficient, and is applicable to improving the nitrogen removal efficiency of the anaerobic ammonium oxidation process in environments with low iron concentrations such as municipal wastewater and natural surface water bodies.

[0016] (5) Introducing tea polyphenol-Fe 3+ into the anaerobic ammonium oxidation system is beneficial to promoting the absorption of ferric iron by anaerobic ammonium-oxidizing bacteria, enhancing the biological utilization of iron elements in the system, thereby promoting the Anammox and DNRA metabolisms of anaerobic ammonium-oxidizing bacteria and strengthening the nitrogen removal efficiency of the anaerobic ammonium oxidation system, which has guiding significance for the engineering application of anaerobic ammonium oxidation. Brief Description of the Drawings

[0017] Figure 1It is a graph showing the changes in intracellular iron content and NRE in the anaerobic ammonium oxidation reactors of Examples 1 to 7 and Comparative Example 1;

[0018] Figure 2 It is a graph showing the changes in the nitrogen concentration of influent and effluent and NRE with the running time in the anaerobic ammonium oxidation reactor of Comparative Example 2;

[0019] Figure 3 It is a graph showing the changes in the nitrogen concentration of influent and effluent and NRE with the running time in the anaerobic ammonium oxidation reactor of Comparative Example 3;

[0020] Figure 4 It is a graph showing the changes in the nitrogen concentration of influent and effluent and NRE with the running time in the anaerobic ammonium oxidation reactor of Example 8;

[0021] Figure 5 It is a graph showing the composition of anaerobic ammonium oxidizing bacteria in the sequencing batch (SBR) reactors of Example 8, Comparative Examples 2 to 3 of the present invention during inoculation of anaerobic ammonium oxidation sludge and in Stage III;

[0022] Figure 6 It is a graph showing the relative abundances of nitrogen conversion functional genes in the SBR reactors of Example 8, Comparative Examples 2 to 3 above during Stage III;

[0023] Figure 7 It is a graph showing the species contribution of nitrogen conversion functional genes in the SBR reactors of Example 8, Comparative Examples 2 to 3 above during Stage III. Detailed implementation manners

[0024] In the ranges disclosed herein, the endpoints and any values are not limited to the exact ranges or values, and these ranges or values should be understood to include values close to these ranges or values. For numerical ranges, between the endpoint values of each range, between the endpoint values of each range and individual point values, and between individual point values, they can be combined with each other to obtain one or more new numerical ranges, and these numerical ranges should be regarded as specifically disclosed herein.

[0025] In the present invention, "NER" represents the total nitrogen removal rate of the anaerobic ammonium oxidation system; "Anammox" represents the anaerobic ammonium oxidation pathway; "DNRA" represents the dissimilatory nitrate reduction to ammonium pathway; " Candidatus Brocadia, Candidatus Jettenia, Candidatus Kuenenia" represents three different genera of anaerobic ammonium oxidizing bacteria; "HZS" represents hydrazine synthase; "HDH" represents hydrazine dehydrogenase; "NAP" represents periplasmic dissimilatory nitrate reductase; "NADH" represents reduced coenzyme I; "NIR" represents cytoplasmic NADH-dependent nitrite reductase; "NRF" represents "periplasmic dissimilatory nitrite reductase".

[0026] As described above, the first aspect of the present invention provides a method for synergistically enhancing nitrogen removal in an anaerobic ammonium oxidation system based on tea polyphenols and ferric iron, and the method includes:

[0027] In an anaerobic ammonium oxidation reactor, anaerobic ammonium oxidation sludge is inoculated, wastewater is added, and then tea polyphenols-Fe3+ is added to treat the wastewater.

[0028] Preferably, the dosing concentration of the anaerobic ammonium oxidation sludge is 2000 mg SS / L.

[0029] More preferably, the inoculated anaerobic ammonium oxidation sludge contains Candidatus Brocadia and Candidatus Jettenia of the genus Anaerobic ammonium oxidizing bacteria.

[0030] Preferably, the preparation method of the tea polyphenols-Fe3+ is to complex tea polyphenols and FeCl3 with a molar concentration ratio of 1:1.

[0031] More preferably, the continuous dosing concentration of the tea polyphenols-Fe3+ is 12.5 - 150 μmol / L.

[0032] Preferably, in the short-term startup stage, the continuous dosing concentration of the tea polyphenols-Fe3+ is 25 μmol / L; in the long-term stable operation stage, the continuous dosing concentration of the tea polyphenols-Fe3+ is 12.5 μmol / L; wherein, the short-term startup stage is preferably 1 - 30 days, and the long-term stable operation stage is preferably > 30 days.

[0033] Preferably, the temperature in the anaerobic ammonium oxidation reactor is controlled at 30 - 35 °C, the pH is 7.8 ± 0.5, and the dissolved oxygen is < 0.1 mg / L.

[0034] In the present invention, the technical principle of synergistically enhancing nitrogen removal in the anaerobic ammonium oxidation system based on tea polyphenols and ferric iron is as follows: Tea polyphenols can promote the absorption of ferric iron by anaerobic ammonium oxidizing bacteria, enhancing the biological utilization of iron elements in the anaerobic ammonium oxidation system. The iron elements absorbed by anaerobic ammonium oxidizing bacteria can be used to synthesize functional enzymes for the Anammox process such as HZS and HDH, as well as functional enzymes for the DNRA process such as NAP, NIR, and NRF, enhancing the Anammox and DNRA metabolic activities of anaerobic ammonium oxidizing bacteria, while promoting the coupling of the two nitrogen conversion pathways, synergistically improving the total nitrogen removal rate, and enhancing the nitrogen removal efficiency of the anaerobic ammonium oxidation system.

[0035] The present invention will be described in detail below through examples. In the following examples, unless otherwise specified, the raw materials are all commercially available products.

[0036] Example 1

[0037] Take 2000 mg of anaerobic ammonium oxidation sludge per liter (SS / L) and place it in a 100 mL serum bottle. Add 100 mL of synthetic wastewater to form an anaerobic ammonium oxidation reactor, and continuously add tea polyphenol-Fe with a concentration of 25 μmol / L. 3+ Treat the wastewater.

[0038] Before inoculation, the anaerobic ammonium oxidation sludge is uniformly pretreated for about 45 days. During this period, no iron element and tea polyphenol are added to the influent water, and other components are added normally to exclude the interference of iron element in the inoculated sludge as much as possible.

[0039] The synthetic wastewater is artificially prepared in the laboratory. In the synthetic wastewater, the ammonia nitrogen concentration is 50 mg / L, the nitrite nitrogen concentration is 60 mg / L, the pH is 7.5 ± 0.1, and the dissolved oxygen is < 0.1 mg / L.

[0040] The anaerobic ammonium oxidation reactor operates in the SBR mode for 7 days, with 2 cycles per day. The duration of a single cycle is 12 h. The temperature is controlled at 32 - 34 °C and the rotation speed is 180 rpm by a constant temperature shaker.

[0041] The synthetic wastewater in the anaerobic ammonium oxidation reactor is changed every half day, and the water change ratio is 100%.

[0042] The tea polyphenol-Fe 3+ is formed by complexing tea polyphenol and Fe with a molar ratio of 1:1. 3+ Complexed.

[0043] Example 2

[0044] Carry out according to the method of Example 1. The difference is that the continuous addition concentration of the tea polyphenol-Fe 3+ is 12.5 μmol / L, and the remaining steps and parameters are the same as those in Example 1.

[0045] Example 3

[0046] Carry out according to the method of Example 1. The difference is that the continuous addition concentration of the tea polyphenol-Fe 3+ is 37.5 μmol / L, and the remaining steps and parameters are the same as those in Example 1.

[0047] Example 4

[0048] Carry out according to the method of Example 1. The difference is that the continuous addition concentration of the tea polyphenol-Fe 3+ is 50 μmol / L, and the remaining steps and parameters are the same as those in Example 1.

[0049] Example 5

[0050] Carry out according to the method of Example 1. The difference is that the tea polyphenol-Fe3+ The continuous dosing concentration of

[0051] Example 6

[0052] was carried out according to the method of Example 1, except that the continuous dosing concentration of the tea polyphenol-Fe 3+ was 75 μmol / L, and the remaining steps and parameters were the same as those in Example 1.

[0053] Example 7

[0054] was carried out according to the method of Example 1, except that the continuous dosing concentration of the tea polyphenol-Fe 3+ was 100 μmol / L, and the remaining steps and parameters were the same as those in Example 1.

[0055] Comparative Example 1

[0056] was carried out according to the method of Example 1, except that the tea polyphenol-Fe 3+ was not added, and the remaining steps and parameters were the same as those in Example 1.

[0057] Example 8

[0058] 2000 mg SS / L of anaerobic ammonium oxidation sludge was placed in an SBR reactor with an effective volume of 1 L, 1 L of synthetic wastewater was added, and the tea polyphenol-Fe 3+ was continuously dosed during Stage I to Stage III for wastewater treatment;

[0059] The anaerobic ammonium oxidation sludge was pretreated for about 45 days before inoculation. During this period, iron elements and tea polyphenols were not added to the influent water, and other components were added normally to exclude the interference of iron elements in the inoculated sludge as much as possible;

[0060] The ammonia nitrogen concentration in the wastewater was 100 mg / L, the nitrite nitrogen concentration was 120 mg / L, the pH was 7.5 ± 0.1, and the dissolved oxygen was <0.1 mg / L;

[0061] The SBR reactor was operated for 75 days, with 3 cycles per day, and the duration of a single cycle was 7 h. The temperature was controlled at 32 - 34 °C by water bath heating, and the stirring speed was 30 rpm;

[0062] The wastewater in the SBR reactor was replaced every 8 h, and the water replacement ratio was 50%;

[0063] Stage I was from the 1st day to the 30th day, and in Stage I, the dosing concentration of the tea polyphenol-Fe 3+ in the SBR reactor was 25 μmol / L;

[0064] The second stage is from the 31st day to the 45th day, and in the second stage, the dosage concentration of the tea polyphenol-Fe in the SBR reactor is 25 μmol / L; 3+

[0065] The third stage is from the 46th day to the 75th day, and in the third stage, the dosage concentration of the tea polyphenol-Fe in the SBR reactor is 12.5 μmol / L; 3+

[0066] The tea polyphenol-Fe 3+ is formed by complexing tea polyphenol and Fe with a concentration molar ratio of 1:1 3+

[0067] Comparative Example 2

[0068] It was carried out according to the method of Example 8, except that tea polyphenol-Fe 3+ was not added, and the remaining steps and parameters were the same as those in Example 8.

[0069] Comparative Example 3

[0070] It was carried out according to the method of Example 8, except that FeCl3 was used to replace the tea polyphenol-Fe 3+ , and FeCl3 with a concentration of 25 μmol / L was continuously added during the first to third stages, and the remaining steps and parameters were the same as those in Example 8.

[0071] Test Example 1

[0072] After the experiment, the intracellular iron content and NRE changes in the anaerobic ammonium oxidation reactors of the above Examples 1 to 7 and Comparative Example 1 were detected, and the results are as Figure 1 shown.

[0073] It can be seen from Figure 1 that the NRE and intracellular iron content in the anaerobic ammonium oxidation reactor increased with the increase of the tea polyphenol-Fe 3+ concentration. However, when the tea polyphenol-Fe 3+ concentration was higher than 25 μmol / L, the NRE and intracellular iron content in the anaerobic ammonium oxidation reactor tended to be stable, indicating that the appropriate dosage concentration of the tea polyphenol-Fe 3+ in the anaerobic ammonium oxidation reactor during the short-term startup stage was 25 μmol / L (i.e., the dosage concentration of the tea polyphenol-Fe 3+ in Example 1 of the present invention).

[0074] Moreover, compared with Comparative Example 1 (without adding tea polyphenol-Fe 3+ ), the NRE in the anaerobic ammonium oxidation reactors of Examples 1 to 7 of the present invention increased by 3.2 to 6.7 times, and the intracellular iron content increased by 91% to 188%, indicating that the tea polyphenol-Fe​​​3+ The introduction of promoted the absorption of trivalent iron by anaerobic ammonium oxidizing bacteria, and then anaerobic ammonium oxidizing bacteria used iron to enhance the metabolism of Anammox, DNRA, etc., significantly enhancing the denitrification efficiency in the anaerobic ammonium oxidation reactor.

[0075] Test Example 2

[0076] During the operation of the reactor, the denitrification efficiency in the SBR reactor of the above-mentioned Example 8 and Comparative Examples 2 and 3 was detected. The results are as follows: Figures 2 - 4 shown.

[0077] Depend on Figures 2 - 4 It can be seen that in stage I, adding 25μmol / L tea polyphenols-Fe 3 + After that, in Example 8 of the present invention, NRE first increased rapidly and then stabilized at 87.7%; however, in stage II, long-term addition of 25 μmol / L tea polyphenols-Fe 3+ It is not conducive to the stable operation of the anaerobic ammonium oxidation system, causing its NRE to drop to 68.4%; in stage III, tea polyphenols-Fe 3+ After the addition concentration was reduced to 12.5 μmol / L, the denitrification efficiency of Example 8 of the present invention recovered rapidly, and NRE finally stabilized to 88.8%, indicating that in the long-term stable operation stage, tea polyphenols-Fe 3+ The appropriate dosage concentration is 12.5 μmol / L.

[0078] Secondly, the denitrification performance in the reactors of Comparative Example 2 and Comparative Example 3 was maintained at a low level, with the NRE in the reactor of Comparative Example 2 being 12.2% and the NRE in the reactor of Comparative Example 3 being 13.5%, indicating that the addition of Fe alone 3+ The denitrification efficiency of the anaerobic ammonium oxidation system cannot be effectively improved. However, in stage III, the NRE in the reactor of Example 8 of the present invention can be stabilized at 88.8%, and at the same time, ∆NO3 − −N / ∆NH4 + −N ratio is 0.23, which is significantly lower than that of comparative example 2 (∆NO3 − −N / ∆NH4 + −N ratio is 0.27) and comparative example 3 (∆NO3 − −N / ∆NH4 + −N ratio is 0.27), indicating that tea polyphenols-Fe 3+ The addition of anammox promoted the metabolism of Anammox and DNRA at the same time, thereby enhancing the denitrification efficiency of the anaerobic ammonium oxidation system.

[0079] Test Example 2

[0080] During the operation of the reactor, the compositions of anaerobic ammonium-oxidizing bacteria in the inoculated anaerobic ammonium-oxidizing sludge and the SBR reactors of Example 8 and Comparative Examples 2 to 3 in Stage III were respectively detected. The results are as Figure 5 shown.

[0081] It can be seen from Figure 5 that the inoculated anaerobic ammonium-oxidizing sludge contains Candidatus Brocadia, Candidatus Jettenia, Candidatus Kuenenia, three genera of anaerobic ammonium-oxidizing bacteria, and Candidatus Brocadia is the main one (accounting for 80%). Compared with the inoculated sludge, due to the lack of directly available dissolved divalent iron in the reactors of Comparative Example 2 and Comparative Example 3, the absolute abundance (2×10 9 →6×10 7 、3×10 7 copies / g VSS) and relative abundance (16%→4%, 2%) of anaerobic ammonium-oxidizing bacteria decreased significantly; while in the reactor of Example 8 of the present invention, the absolute abundance (9×10 8 copies / g VSS) and relative abundance (12%) of anaerobic ammonium-oxidizing bacteria remained basically unchanged, but Candidatus Jettenia was enriched, and the proportion in anaerobic ammonium-oxidizing bacteria increased significantly from 17% to 39%, indicating that tea polyphenol-Fe 3+ helps to maintain the proliferation of anaerobic ammonium-oxidizing bacteria in the anaerobic ammonium-oxidizing system, especially Candidatus the proliferation of Jettenia.

[0082] Test Example 2

[0083] During the operation of the reactor, in Stage III, the relative abundances of nitrogen conversion functional genes and the species contributions of nitrogen conversion functional genes in the SBR reactors of Example 8 and Comparative Examples 2 to 3 were respectively detected. The results are respectively as Figure 6 and Figure 7 shown.

[0084] It can be seen from Figure 6 that compared with Comparative Example 2 and Comparative Example 3, the relative abundances of the hzs , hdh genes related to Anammox metabolism, and the napAB , nirBD , nrfAH genes related to DNRA metabolism in Example 8 of the present invention all increased significantly, indicating that tea polyphenol-Fe 3+ strengthened the Anammox and DNRA metabolisms of the anaerobic ammonium-oxidizing system.

[0085] Moreover, the genus of anaerobic ammonium-oxidizing bacteriaCandidatus Brocadia and Anaerobic ammonium oxidizing bacteria Candidatus Jettenia is the main microorganism in the system that conducts Anammox ( hzs and hdh gene) and DNRA ( nrfAH gene) metabolism. As Figure 7 can be seen, in the reactor of Comparative Example 2, anaerobic ammonium oxidizing bacteria contributed 95% hzs gene, 77% hdh gene, 5% nrfAH gene; in the reactor of Comparative Example 3, anaerobic ammonium oxidizing bacteria contributed 88% hzs gene, 57% hdh gene, 3% nrfAH gene; in the reactor of Example 8 of the present invention, anaerobic ammonium oxidizing bacteria contributed 99% hzs gene, 99% hdh gene, 17% nrfAH gene. Compared with Comparative Example 2 and Comparative Example 3, the contribution of anaerobic ammonium oxidizing bacteria to the functional genes of Anammox and DNRA pathways in Example 8 of the present invention increased significantly, indicating that tea polyphenol-Fe 3+ mainly promoted the Anammox and DNRA metabolism of anaerobic ammonium oxidizing bacteria.

[0086] In summary, as Figures 1 - 7 can be seen, tea polyphenol-Fe 3+ can enhance the Anammox and DNRA metabolism of anaerobic ammonium oxidizing bacteria by promoting the absorption of ferric iron by anaerobic ammonium oxidizing bacteria, and significantly strengthen the nitrogen removal efficiency of the anaerobic ammonium oxidation system. Among them, the appropriate dosing concentration of tea polyphenol-Fe 3+ is 25 μmol / L in the short-term startup stage and 12.5 μmol / L in the long-term stable operation stage.

[0087] The preferred embodiments of the present invention have been described in detail above. However, the present invention is not limited thereto. Within the scope of the technical concept of the present invention, various simple modifications can be made to the technical solutions of the present invention, including any other suitable combination of each technical feature. These simple modifications and combinations should also be regarded as the content disclosed by the present invention and fall within the protection scope of the present invention.

Claims

1. A method for denitrification of anaerobic ammonium oxidation system based on tea polyphenols and trivalent iron, characterized in that: The method includes: In the anaerobic ammonium oxidation reactor, anaerobic ammonium oxidation sludge is inoculated, wastewater is added, and then tea polyphenols-Fe are added. 3+ , treat wastewater; The tea polyphenols-Fe 3+ The preparation method is to complex tea polyphenols and FeCl3 with a molar concentration of 1:

1.

2. The method according to claim 1, characterized in that: The addition concentration of the anaerobic ammonium oxidation sludge is 2000 mgSS / L.

3. The method according to claim 1, characterized in that: The inoculated anaerobic ammonium oxidation sludge contains anaerobic ammonium oxidizing bacteria Candidatus Brocadia and Anammox bacteria Candidatus Jettenia.

4. The method according to claim 1, characterized in that: The tea polyphenols-Fe 3+ The continuous addition concentration is 12.5~150μmol / L.

5. The method according to claim 1, characterized in that In the short-term startup phase, the tea polyphenols-Fe 3+ The continuous addition concentration is 25 μmol / L; in the long-term stable operation stage, the tea polyphenol-Fe 3+ The continuous addition concentration is 12.5 μmol / L; wherein the short-term startup phase is 1 to 30 days, and the long-term stable operation phase is greater than 30 days.

6. The method according to claim 1, characterized in that The temperature in the anaerobic ammonium oxidation reactor was controlled at 30~35℃, the pH at 7.8±0.5, and the dissolved oxygen <0.1mg / L.

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