A detection device and method for detecting a bacterial culture

Abstract

The application discloses a kind of detection device and detection method of strain culture, belong to strain culture technical field.A kind of detection device of strain culture, including box and box cover;The inner bottom of the box is provided with culture cabin, and is provided with one or more sampling disc groups;The lower end of the box cover is provided with the extraction structure that cooperates with sampling disc group.The application, cooperation is formed the box and box cover of closed state, strain culture is carried out in inside, is not disturbed by outside;Sampling from the inside of box, and after sampling is finished, it is closed again, the whole operation is disturbed by outside factor little;Setting has multilayer morphology's sampling disc group, can simultaneously sample multilayer;Multiple sampling disc groups can be optionally set, can simultaneously sample multiple, further enrich sampling point, guarantee the reliable, effective of detection data;Center groove is opened on sampling tray, strain culture in cultivation process preferentially covers center groove, relatively more concentrated, facilitate detection.

Description

Technical Field

[0001] This invention relates to the field of microbial culture technology, and in particular to a detection device and method for microbial culture. Background Technology

[0002] In agriculture, the application of beneficial microbial communities is of paramount importance. Specific beneficial microbial communities can play a significant role in soil improvement, creating a more suitable soil environment for crop growth through various means such as improving soil structure, regulating soil pH, and enhancing soil fertility. Simultaneously, they demonstrate remarkable efficacy in promoting crop yields, increasing both crop output and quality, and bringing considerable economic benefits to agricultural production. Therefore, these beneficial microbial communities are widely used in agricultural production.

[0003] In the production of relevant microbial strains, accurate monitoring of the strain culture and ensuring the final culture quality are crucial. To achieve this goal, it is necessary to conduct regular or irregular monitoring of the strain culture. During the culture process, because the growth status of the strain may change with time and environmental variations, multiple sampling operations are often required to understand the dynamics of the strain culture in a timely manner.

[0004] In existing technologies, the common method for culturing and detecting microbial strains is to directly sample from the surface of the culture structure, such as an incubator, and then perform analysis. However, this sampling method has significant limitations. First, it is extremely limited, only obtaining samples from the surface of the incubator and failing to provide multi-level sampling inside the incubator, making it difficult to comprehensively reflect the actual cultivation status of the strains at different depths and locations. Second, if the incubator is left open for an extended period during sampling, the microbial strains inside may come into contact with external bacteria; external bacteria may enter the incubator and contaminate the strains. This contamination not only affects the normal growth of the strains but may also lead to mutations or death. Furthermore, contamination can severely impact the test results, even causing significant discrepancies between the test results and the actual results, thus affecting the accurate assessment of the strain cultivation status and subsequent production decisions.

[0005] Therefore, the applicant made improvements. Summary of the Invention

[0006] The purpose of this invention is to solve the above-mentioned problems in the prior art by providing a detection device and method for bacterial culture.

[0007] To achieve the above objectives, the present invention adopts the following technical solution:

[0008] A detection device for bacterial culture includes a box body and a box cover; the bottom of the box body is provided with a culture chamber and one or more sampling trays are placed thereon; the lower end of the box cover is provided with an extraction structure that cooperates with the sampling trays.

[0009] In some embodiments, the sampling tray assembly consists of a vertically arranged central rod and multiple sampling trays coaxially arranged and fixedly connected to the central rod.

[0010] In some embodiments, the extraction structure includes: an inverted cylinder;

[0011] The bottom layer of the sampling disk assembly is provided with a disk or magnetic ring; the lower end of the inverted cylinder is provided with a magnetic bottom ring that cooperates with the disk or magnetic ring.

[0012] In some embodiments, the extraction structure further includes: one or more telescopic rods installed on the inner bottom of the box cover; a cylinder seat is fixedly provided at the lower end of the telescopic rod; and the lower end of the cylinder seat is connected to the inverted cylinder.

[0013] In some embodiments, the cylinder base is magnetic; a magnetic attracting plate is provided on the top of the inverted cylinder.

[0014] In some embodiments, an equipment compartment with increased internal dimensions is provided above the culture chamber; the equipment compartment is connected to the culture chamber, and a positioning step is formed at the connection point;

[0015] A reel compartment is provided on one side of the equipment compartment, and a take-up reel is provided inside the reel compartment. A sealing cloth is wound around the take-up reel.

[0016] In some embodiments, a clamping mechanism is provided inside the equipment compartment;

[0017] The clamping mechanism includes: a lower pressure bar respectively provided for the positioning steps on the four sides; the lower pressure bar is vertically driven by a second telescopic rod provided on the inner wall of the equipment compartment.

[0018] In some embodiments, the equipment compartment is provided with one or more sterilization components.

[0019] A detection method for bacterial strain culture includes the following steps:

[0020] S1: Preparation for bacterial culture;

[0021] S2, the bacterial culture process;

[0022] S3. Microbial sampling;

[0023] S4. Microbial strain detection.

[0024] In some embodiments, step S1 includes the following steps:

[0025] The equipment is cleaned, and the culture chamber and equipment chamber are sterilized using sterilization components to ensure a clean and sterile internal environment.

[0026] In some embodiments, step S2 includes the following steps:

[0027] The culture chamber is filled with bacterial culture, and the corresponding sampling trays are placed there.

[0028] After that, wait for the bacterial strain to be cultured to the required time point for sampling and testing.

[0029] In some embodiments, in step S2, the culture chamber is in a closed state or connected to the equipment chamber.

[0030] In some embodiments, step S5 includes the following steps:

[0031] Ensure the cover is folded up;

[0032] Control the descent of the extraction structure and coordinate with the sampling disk assembly;

[0033] After the extraction structure drives the sampling tray assembly to the top, the control of the sealing cloth movement, in conjunction with the pressing mechanism, temporarily seals the culture chamber;

[0034] Next, open the lid and remove the inverted cylinder.

[0035] Compared with the prior art, the present invention provides a detection device and detection method for bacterial culture, which has the following beneficial effects.

[0036] 1. This invention features a box and lid that work together to form a closed system, allowing the microbial culture to be cultivated internally without external interference. Sampling is taken from inside the box, and the box is resealed after sampling, minimizing external interference during the overall operation. A multi-layered sampling tray assembly allows for simultaneous sampling of multiple layers. Multiple sampling tray assemblies can be optionally provided, enabling simultaneous sampling from multiple locations, further enriching the sampling points and ensuring the reliability and validity of the test data. A central groove is provided on the sampling tray, allowing the microbial culture to preferentially cover the central groove during cultivation, resulting in a more concentrated distribution and easier detection.

[0037] 2. In this invention, an inverted cylinder is used to completely enclose and cover the sampling trays of each layer, bringing the sampling tray assembly out to form a closed structure. This further reduces the influence of the external environment and improves the accuracy of the data. The bottom layer of the sampling tray assembly is equipped with a disk or magnetic ring, and the lower end of the inverted cylinder is equipped with a magnetic bottom ring for automatic docking, which is convenient and efficient. A flexible sealing gasket / ring is provided at the lower end of the magnetic bottom ring, with the disk acting as a bottom cover, making the inverted cylinder a sealed structure. The sampled colonies are sealed, and the sealing effect is excellent.

[0038] 3. In this invention, during the upward sampling process, the lid is always in a closed state with the chamber body, so there will be no external environmental interference or impact on sampling; the cylinder base is equipped with a magnetic suction piece, which makes it easy to quickly remove the inverted cylinder connected to the sampling tray assembly; a temporary sealing component is set to further reduce the impact on the culture chamber during sampling and testing; and a clamping mechanism is set to improve the sealing performance of the temporary seal.

[0039] 4. In this invention, the sealing cloth has a three-layer structure, including: an outer gauze, a filter cloth, and an inner gauze from the outside to the inside, which has a reliable and effective bacterial filtration effect, and also has a ventilation function; a sterilization component is set to treat the inside; the ultraviolet germicidal lamp is movable for better coverage.

[0040] Other advantages, objectives and features of the invention will be set forth in part in the description which follows; and in part will be apparent to those skilled in the art upon examination of the following description; or may be learned from practice of the invention. Attached Figure Description

[0041] Figure 1 This is a schematic diagram of the structure of the present invention.

[0042] Figure 2 This is a cross-sectional structural diagram of the present invention.

[0043] Figure 3 This is a structural diagram of the box.

[0044] Figure 4 This is a cross-sectional structural diagram of the box.

[0045] Figure 5 This is a top-view sectional structural diagram of the box.

[0046] Figure 6 This is a schematic diagram of the sampling container.

[0047] Figure 7 This is a schematic diagram of the sampling disk assembly.

[0048] In the picture:

[0049] 1. Box body;

[0050] 2. Incubation chamber;

[0051] 3. Equipment compartment;

[0052] 4. Roller compartment;

[0053] 5. Sampling tray assembly; 51. Center rod; 52. Sampling tray; 53. Magnetic disk;

[0054] 6. Locate the steps;

[0055] 7. Rewinding shaft; 71. Sealing fabric; 72. Rewinding motor;

[0056] 8. Clamping mechanism; 81. Second telescopic rod; 82. Lower pressure bar;

[0057] 9. Inner groove;

[0058] 10. Sterilization component; 101. First motor; 102. Second lead screw; 103. Lamp holder; 104. Ultraviolet germicidal lamp;

[0059] 11. Box lid;

[0060] 12. Telescopic rod; 13. Base;

[0061] 14. Inverted cylinder; 141. Cylinder body; 142. Magnetic bottom ring; 143. Magnetic plate;

[0062] 15. Guide groove; 16. Guide rod;

[0063] 17. Moving slot; 18. Second motor; 19. First lead screw;

[0064] 20. Tie bar; 21. Positioning groove; 22. Positioning rod. Detailed Implementation

[0065] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments.

[0066] Reference Figure 1-7 A detection device for bacterial culture includes a box 1 and a lid 11 that are fitted together to form a closed state; bacterial culture is carried out inside the box 1, and the lid 11 is fitted to the box 1 to perform sampling and detection of the bacterial culture.

[0067] like Figure 1 As shown, the box body 1 and the box cover 11 are independent structures; when the box cover 11 is closed from top to bottom, the two form a closed shape, and the bacteria can be cultured inside without being disturbed by the outside; when sampling and testing are required, samples are taken from the inside of the box body 1 through the box cover 11; and after sampling is completed, the box is closed again; the bacteria can continue to be cultured inside the box body 1.

[0068] In some embodiments, a culture chamber 2 is provided at the bottom of the box 1, and one or more sampling trays 5 with a multi-layered shape are placed in the culture chamber 2; at the same time, an extraction structure that cooperates with the sampling trays 5 is provided at the lower end of the box cover 11 to lift the sampling trays 5 and take them out to the outside.

[0069] like Figure 2 , 4As shown; inside the culture chamber 2, there are two sampling trays 5 for detecting bacterial species.

[0070] It should be noted that the extraction structure has a first form that is far away from the sampling plate group 5, and a second form that is close to the sampling plate group 5 and then removed from it. In the first form, the extraction structure and the sampling plate group 5 do not interfere with each other, and the bacteria naturally multiply on the sampling plate group 5. In the second form, the two work together to remove the sampling plate group 5 for testing.

[0071] Specifically, such as Figure 2 , 7 As shown; in order to form a multi-layered structure, the sampling tray group 5 consists of: a vertically set central rod 51, and a multi-layer sampling tray 52 coaxially set and fixedly connected to the central rod 51; the spacing and height of the sampling trays 52 are set according to the actual sampling requirements.

[0072] Understandably, the bottom sampling tray 52 serves as a base, providing stable support.

[0073] like Figure 7 As shown; the sampling tray group 5 includes three sampling trays 52 arranged from top to bottom; a magnetic ring is provided on the outer ring side of the bottom sampling tray 52.

[0074] Preferably, the sampling tray 52 has a central groove, which facilitates the inoculation, placement, and aggregation culture of the strains. It also allows the strains to preferentially cover the central groove during the culture process, making them more concentrated and easier to detect.

[0075] The corresponding extraction structure includes: inverted cylinder 14.

[0076] The inverted cylinder 14 includes a cylinder 141 with an open bottom and a closed top.

[0077] When preparing for testing, the inverted cylinder 14 moves downward to cover and enclose the sampling trays 52 of each layer inside it, and then moves upward to bring the sampling tray group 5 out of the culture chamber 2.

[0078] Understandably, the inner diameter of the inverted cylinder 14 should be larger than the outer diameter of the sampling tray 52 "except for the bottom layer" to ensure smooth downward movement.

[0079] Correspondingly, we also need to set up a connection structure between the two (inverted cylinder 14 and sampling disk group 5) to achieve synchronous movement; considering the built-in nature of sampling disk group 5 (placed in a closed box), and in order to form a form that does not require manual operation and reduce external interference, we set up a magnetic suction form to achieve adaptive cooperation between the extraction structure and sampling disk group 5.

[0080] Specifically, a disk 53 or a magnetic ring is provided at the bottom layer of the sampling disk group 5; correspondingly, a magnetic bottom ring 142 that cooperates with the disk 53 or the magnetic ring is provided at the lower end of the inverted cylinder 14.

[0081] It is understandable that when set to disk 53 mode, the disk structure is located below the bottom sampling tray 52 and does not affect the bacterial culture of the bottom sampling tray 52; when set to magnetic ring mode, the magnetic ring structure is located on the outer ring side of the bottom sampling tray 52.

[0082] In addition, it should be noted that the disk 53 or the magnetic ring is made of a material that is magnetic; or, it is made of a material that is not magnetic and can be attracted by the magnetic bottom ring 142. Simply put, as long as it can cooperate with the magnetic bottom ring 142 to attract each other, it is acceptable.

[0083] Furthermore, a flexible sealing gasket / ring is provided at the lower end of the magnetic bottom ring 142.

[0084] When in the working state, the magnetic bottom ring 142 attracts the disk 53 and presses the sealing gasket / ring in the middle; the disk 53 becomes the bottom cover, making the inverted cylinder 14 a sealed structure, and the sampled colonies are sealed; then, the sampling disk assembly 5 is taken out along with the extraction structure for testing.

[0085] It is understandable that the sampling disk assembly 5 has a certain weight; under normal conditions, the sampling disk assembly 5 is in a stable position; when the extraction structure approaches, the magnetic bottom ring 142 and the disk 53 attract each other and pull the sampling disk assembly 5 away.

[0086] It should be noted that the magnetic bottom ring 142 and the disk 53 or magnetic ring should have an adsorption force greater than the weight of the sampling disk group 5 to ensure that it remains in a stable and effective adsorption state during movement.

[0087] Furthermore, the magnetic bottom ring 142 is electromagnetic in nature; it can be controlled to have magnetic force or lose magnetic force according to the working state, making it more flexible and convenient; it can also be conveniently put back into the culture chamber 2 after the sampling plate group 5 is tested, so that the culture can continue.

[0088] It should be noted that the sampling disk assembly 5 is placed at a designated position in the culture chamber 2, corresponding to the extraction structure, in order to achieve adsorption.

[0089] Preferably, markings are set on the inner bottom surface of the culture chamber 2 to assist in the alignment and placement of the sampling tray group 5.

[0090] In some embodiments, we further provide a moving component for the extracted structure to enable switching between two forms of the extracted structure.

[0091] Specifically, the extraction structure also includes: one or more telescopic rods 12 installed on the inner bottom of the box cover 11; a cylinder seat 13 is fixedly installed at the lower end of the telescopic rod 12; the lower end of the cylinder seat 13 is connected to the inverted cylinder 14; the inverted cylinder 14 is adjusted up and down by driving the telescopic rod 12.

[0092] Preferably, the telescopic rod 12 is electrically powered, which facilitates direct control from the outside.

[0093] It should be noted that the telescopic rod 12 and the cylinder seat 13 correspond one-to-one with the inverted cylinder 14; and each telescopic rod 12 is independently controlled; when sampling and testing, one sampling disc group 5 or multiple sampling disc groups 5 can be taken out.

[0094] It should also be noted that during the upward sampling process, the lid 11 remains closed to the body 1, preventing external environmental interference and ensuring the sampling is not affected. Figure 6 As shown, one extraction structure is in a downward position and the other extraction structure is in an upward position. In this step, the inverted cylinder 14 and the sampling plate group 5 are in a closed box and work together. The inverted cylinder 14 and the sampling plate group 5 are also in a closed state to temporarily store the bacteria to be tested.

[0095] Of course, the lid 11 needs to be removed afterward to take out the sampling tray assembly 5 (the sampling tray assembly 5 and the inverted cylinder 14 are together).

[0096] In some embodiments, in order to further reduce contamination, environmental interference and other factors before reaching the detection site, we set the sampling tray group 5 and the inverted cylinder 14 as an integrated sampling unit; therefore, we need to control the connection state between the inverted cylinder 14 and the cylinder base 13.

[0097] Specifically, the cylinder base 13 is magnetic; a magnetic absorbing piece 143 is provided on the top of the inverted cylinder 14; the magnetic absorbing piece 143 is attracted by the cylinder base 13; making it convenient to quickly remove the inverted cylinder 14 connected to the sampling tray assembly 5.

[0098] It is understandable that the cylinder base 13 may have an embedded magnetic structure such as a magnet; or it may be made of a magnetic material. In addition, the attraction between the cylinder base 13 and the magnetic plate 143 should be greater than the overall weight of the inverted cylinder 14 and the sampling disk assembly 5, so that it will not fall off when not pulled by external force.

[0099] Therefore, after removing the box cover 11, we can directly remove the inverted cylinder 14 (by applying external force to overcome the suction force, the inverted cylinder 14 can be removed from the cylinder base 13).

[0100] Preferably, the cylinder base 13 has a groove at the bottom to fit the lower groove at the top of the inverted cylinder 14; after the inverted cylinder 14 is inserted, it fits tightly.

[0101] In some embodiments, a temporary sealing component is also provided to further reduce the impact on the culture chamber 2 during sampling and testing.

[0102] Specifically, an equipment compartment 3 with increased internal dimensions is provided above the culture chamber 2; the equipment compartment 3 is connected to the culture chamber 2, and a positioning step 6 is formed at the connection; at the same time, a roll compartment 4 is provided on one side of the equipment compartment 3, and the roll compartment 4 is connected to the equipment compartment 3 through a roll channel; the inside of the roll compartment 4 is provided with a take-up shaft 7, and a sealing cloth 71 is wound on the take-up shaft 7, and a take-up motor 72 is provided at one end of the take-up shaft 7 for driving; the sealing cloth 71 enters above the positioning step 6 after passing through the roll channel, and extends to the inner wall of the equipment compartment 3, thereby temporarily sealing the culture chamber 2 below.

[0103] It is understandable that the winding shaft 7 drives the closed fabric 71 to shrink and coil; however, the extension and covering of the closed fabric 71 require additional power components.

[0104] Specifically, the end of the sealing cloth 71 is fixed to the pull strip 20; on the inner wall of the front or rear side of the equipment compartment 3, corresponding to the position above the positioning step 6, a moving groove 17 is provided along the moving direction of the sealing cloth 71; a second motor 18 is provided in the moving groove 17, and the output end of the second motor 18 is connected to a rotatable first lead screw 19; a screw hole is provided at the end of the pull strip 20, which cooperates with the first lead screw 19 for transmission; driven by the first lead screw 19, the pull strip 20 is driven to extend the sealing cloth 71.

[0105] Understandably, when the pull bar 20 moves to the end of the first lead screw 19, the sealing cloth 71 covers the culture chamber 2.

[0106] It should be noted that one movable slot 17 can be installed on each of the front and rear inner walls of the equipment compartment 3, and the second motor 18 in the movable slots 17 on both sides is driven synchronously to provide stable and reliable pulling force.

[0107] Of course, it is also possible to configure one side as a motor-driven first lead screw 19 and the other side as a smooth guide rod. For example... Figure 5 As shown; the equipment compartment 3 is provided with a moving groove 17 on one side and a corresponding positioning groove 21 on the other side; the positioning groove 21 is provided with a positioning rod 22 (smooth rod slide rod) that passes through the pull bar 20; the positioning rod 22 cooperates with the first lead screw 19.

[0108] In some embodiments, in order to further enhance the temporary sealing effect of the sealing cloth 71, a clamping mechanism 8 is also provided.

[0109] Specifically, a clamping mechanism 8 is installed inside the equipment compartment 3.

[0110] The clamping mechanism 8 includes: a lower pressure strip 82 respectively provided on the positioning steps 6 on the four sides.

[0111] The pressure bar 82 is vertically driven by the second telescopic rod 81 set on the inner wall of the equipment compartment 3.

[0112] When closure is required, the pull strip 20 drives the closure cloth 71 to the end, and the lower pressure strip 82 is pressed down by the second telescopic rod 81, pressing the closure cloth 71 onto the positioning step 6; the four lower pressure strips 82 can press the four sides of the closure cloth 71 tightly, improving the closure of the temporary closure.

[0113] In some embodiments, we further designed the form of the closed fabric 71.

[0114] Specifically, the sealing cloth 71 has a three-layer structure, including: an outer gauze, a filter cloth, and an inner gauze from the outside to the inside; the three-layer structure sandwiches the filter cloth between the inner and outer layers to ensure reliable and effective bacterial filtration; it also has a ventilation function.

[0115] In some embodiments, a sterilization component 10 is also provided to take into account the treatment of the culture environment.

[0116] Specifically, one or more sterilization components 10 are installed in the equipment compartment 3.

[0117] The sterilization component 10 includes: an ultraviolet germicidal lamp 104.

[0118] Preferably, the ultraviolet germicidal lamps 104 are arranged in the front-to-back direction.

[0119] Furthermore, the UV germicidal lamp 104 is movable for better coverage.

[0120] It is understandable that the UV germicidal lamps 104 are arranged in the front-to-back direction and moved in the left-to-right direction.

[0121] Specifically, an inner groove 9 is provided on one side of the equipment compartment 3 along its length, and a drive assembly is provided inside the inner groove 9; the ultraviolet germicidal lamp 104 is mounted on the lamp holder 103, and the lamp holder 103 is connected to the drive assembly for transmission; the drive assembly drives the lamp holder 103 to move.

[0122] Preferably, an ultraviolet germicidal lamp 104 is provided on each of the left and right sides of the equipment compartment 3, and each lamp is independently controlled to move; preferably, it is configured as two intermittent inner tanks 9.

[0123] In some embodiments, the drive assembly includes: a first motor 101 installed in the inner groove 9, and a second lead screw 102 driven through the output end of the first motor 101; the second lead screw 102 is threadedly engaged with the end of the lamp holder 103 for transmission.

[0124] Thus, the movement of the ultraviolet germicidal lamp 104 is controlled by the first motor 101; the two ultraviolet germicidal lamps 104 move freely in the equipment chamber 3, and the disinfection range is fully covered with good effect.

[0125] Meanwhile, a guide groove 15 is provided on the side of the inner wall of the equipment compartment 3 away from the inner groove 9, and the guide groove 15 corresponds to the inner groove 9; a guide rod 16 (light rod slide rod) that passes through the lamp holder 103 is provided inside the guide groove 15.

[0126] It should be noted that the sterilization component 10 is positioned above the pull strip 20 and does not affect or interfere with the movement of the sealing fabric 71; at the same time, the sterilization component 10 should cooperate with the lower pressure strip 82 to avoid interference.

[0127] like Figure 4 As shown; when the sterilization component 10 moves, the pressure bar 82 cannot move up or down; correspondingly, when the pressure bar 82 moves up or down, the sterilization component 10 should move to a clearance position. Similarly, when the inverted cylinder 14 moves, the sterilization component 10 should move to a clearance position.

[0128] Specifically, the sterilization component 10 does not move to its left or rightmost ends, and the two downward pressure strips 82 on the left and right sides do not interfere with the sterilization component 10; at the same time, the length of the front and rear downward pressure strips 82 should be less than the distance between the two sterilization components 10 in the avoidance state; so as to... Figure 4 In the avoidance state shown, the lower pressure strips 82 on the front and rear sides can move up and down.

[0129] A method for detecting bacterial strains, using the aforementioned detection device for bacterial strains;

[0130] It includes the following steps:

[0131] S1: Preparation for bacterial culture;

[0132] S2, the bacterial culture process;

[0133] S3. Microbial sampling;

[0134] S4. Microbial strain detection.

[0135] Step S1 includes the following steps:

[0136] The device is cleaned, and the culture chamber 2 and equipment chamber 3 are sterilized by the sterilization component 10 to ensure a clean and sterile internal environment.

[0137] Step S2 includes the following steps:

[0138] Place the bacterial culture into the culture chamber 2 and place the sampling tray group 5 accordingly;

[0139] After that, wait for the bacterial strain to be cultured to the required time point for sampling and testing.

[0140] In step S2, the culture chamber 2 is either in a closed state or connected to the equipment chamber 3.

[0141] Step S3 includes the following steps:

[0142] Ensure that the closed fabric 71 is in a stowed state;

[0143] Control the descent of the extraction structure and coordinate with the sampling disk group 5;

[0144] After the extraction structure drives the sampling plate group 5 to the top, the control of the sealing cloth 71 moves in conjunction with the pressing mechanism 8 to temporarily seal the culture chamber 2;

[0145] Next, open the box cover 11 and remove the inverted cylinder 14.

[0146] Step S4 includes the following steps:

[0147] The inverted cylinder 14 was taken along with the sampling tray group 5 for testing.

[0148] It should be noted that: the sampling tray group 5 is placed at the designated position at the bottom of the culture chamber 2 so that it can cooperate with and adsorb the inverted cylinder 14; after the bacteria have been cultured in the culture chamber 2 for a period of time, some of the bacteria will fall onto the sampling tray group 5 (or can be directly inoculated onto the sampling tray group 5); when the extraction structure drives the sampling tray group 5 to the top, its lower end should be higher than the sealing cloth 71.

[0149] In this invention, a box 1 and a lid 11 are configured to form a closed system, allowing the microbial culture to be cultivated internally without external interference. Sampling is taken from inside the box 1, and the box is resealed after sampling, minimizing external interference during the overall operation. A multi-layered sampling tray group 5 is provided, allowing for simultaneous sampling of multiple layers. Multiple sampling tray groups 5 can be optionally provided, enabling simultaneous sampling at multiple locations, further enriching the sampling points and ensuring the reliability and validity of the test data. A central groove is formed on the sampling tray 52, allowing the microbial culture to preferentially cover the central groove during cultivation, resulting in a more concentrated and easier-to-detect culture. An inverted cylinder 14 encloses and covers each layer of the sampling tray 52, then pulls the sampling tray group 5 out, forming a closed system, further reducing the influence of the external environment and improving data accuracy. A magnetic disk 53 or magnetic ring is provided at the bottom of the sampling tray group 5, and a magnetic bottom ring 142 is provided at the bottom of the inverted cylinder 14 for automatic... The connection is convenient and efficient; a flexible sealing gasket / ring is set at the lower end of the magnetic bottom ring 142, and the disk 53 becomes the bottom cover, making the inverted cylinder 14 a sealed structure, and the sampled colonies are sealed, with a good sealing effect; during the upward sampling process, the box cover 11 is always in a closed state with the box body 1, and there will be no external environmental interference or impact on sampling; the cylinder base 13, together with the magnetic suction plate 143, makes it easy to quickly remove the inverted cylinder 14 connected to the sampling disk assembly 5; a temporary sealing component is set to further reduce the impact on the culture chamber 2 during sampling and testing; a pressing mechanism 8 is set to improve the sealing performance of the temporary seal; the sealing cloth 71 has a three-layer structure, including: outer gauze, filter cloth and inner gauze from the outside to the inside, which has reliable and effective bacterial filtration effect, and also has a ventilation function; a sterilization component 10 is set to treat the inside; the ultraviolet germicidal lamp 104 is movable for better coverage.

[0150] The above description is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the scope of the technology disclosed in the present invention, based on the technical solution and inventive concept of the present invention, should be covered within the scope of protection of the present invention.

[0151] In the description of this specification, the references to terms such as "one embodiment," "some embodiments," "example," "specific example," or "some examples," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the present invention. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. Moreover, without contradiction, those skilled in the art can combine and integrate the different embodiments or examples described in this specification, as well as the features of different embodiments or examples.

[0152] Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention. Those skilled in the art can make changes, modifications, substitutions and variations to the above embodiments within the scope of the present invention.

Claims

1. A device for detecting a culture of a bacterial species, characterized in that, It comprises a box (1) and a box cover (11) which fit to form a closed state; the inner bottom of the box (1) is provided with a culture cabin (2) and one or more sampling disc groups (5) are placed; the lower end of the box cover (11) is provided with an extraction structure which cooperates with the sampling disc group (5); The extraction structure has a first form away from the sampling disc group (5) and a second form close to the sampling disc group (5) and moving it away; in the first form, the extraction structure does not interfere with the sampling disc group (5), and the bacteria on the sampling disc group (5) naturally proliferate; in the second state, the two cooperate to take out the sampling disc group (5) for detection; The sampling disc group (5) forms a multi-level structure; it is composed of a vertically arranged central rod (51) and a plurality of layers of sampling trays (52) coaxially arranged and fixedly connected to the central rod (51); The extraction structure comprises an inverted cylinder (14); the lowermost layer of the sampling disc group (5) is provided with a magnetic disc (53) or a magnetic ring; the lower end of the inverted cylinder (14) is provided with a magnetic suction bottom ring (142) which cooperates with the magnetic disc (53) or the magnetic ring; when preparing for detection, the inverted cylinder (14) moves downward, covers each layer of sampling trays (52) inside, and then moves upward to take out the sampling disc group (5) from the culture cabin (2); The extraction structure further comprises one or more telescopic rods (12) installed on the inner bottom of the box cover (11); the lower end of the telescopic rod (12) is fixedly provided with a cylinder seat (13); the lower end of the cylinder seat (13) is connected with the inverted cylinder (14); The upper position of the culture cabin (2) is provided with an equipment cabin (3) with increased internal dimensions; the equipment cabin (3) is communicated with the culture cabin (2) and forms a positioning step (6) at the connection; one side of the equipment cabin (3) is provided with a reel cabin (4), the inside of the reel cabin (4) is provided with a winding shaft (7), and the winding shaft (7) is wound with a closed cloth (71); The equipment cabin (3) is provided with a pressing mechanism (8); the pressing mechanism (8) comprises a lower pressing strip (82) arranged corresponding to each side of the positioning step (6); the lower pressing strip (82) is vertically driven by a second telescopic rod (81) arranged on the inner wall of the equipment cabin (3).

2. The device for detecting the cultivation of bacteria according to claim 1, wherein A central groove is formed on the sampling tray (52); it is more convenient for bacteria inoculation, placement, and aggregation culture operation; and the bacteria preferentially cover the central groove during the culture process, which is relatively more concentrated and convenient for detection.

3. The device for detecting the cultivation of bacteria according to claim 1, wherein The magnetic suction bottom ring (142) is in electromagnetic form; according to the operation state, it has magnetic force or loses magnetic force; after detecting the sampling disc group (5), it is convenient to put the sampling disc group (5) back into the culture cabin (2) for continuous culture.

4. The device for detecting a culture of bacteria according to claim 1, wherein The sampling disc group (5) and the inverted cylinder (14) are arranged in an integrated sampling form; the cylinder seat (13) has magnetism; the top of the inverted cylinder (14) is provided with a magnetic suction piece (143).

5. The device for detecting the cultivation of bacteria according to claim 1, wherein The equipment cabin (3) is provided with one or more sterilization components (10).

6. A method for detecting a culture of a bacterial species, characterized by, The detection device for bacteria culture of any one of claims 1-5 is adopted; The detection method comprises the following steps: S1: strain culture preparation; S2, strain culture process; S3, strain sampling; S4, strain detection.

7. The method according to claim 6, wherein In step S3, Comprise the following steps: Ensure that the closure cloth (71) is in the storage state; Control the extraction structure to descend and cooperate with the sampling disc group (5); After the extraction structure drives the sampling disc group (5) to rise to the top, control the closure cloth (71) to move and cooperate with the compression mechanism (8), temporarily seal the culture cabin (2); Then, open the box cover (11), and remove the inverted cylinder (14).

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