Thyroid cancer gene detection kit and use method thereof
By designing the connecting rod and track structure of the thyroid cancer gene detection kit, as well as the precise docking of steering gears and directional dials, the problems of loose component connections, solution overflow and impurity interference are solved, and automatic precise injection and efficient detection are achieved.
Patent Information
- Application Number
- CN202510153429.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-02-12
- Publication Date
- 2025-05-02
- Estimated Expiration
- Not applicable · inactive patent
AI Technical Summary
The components of the existing thyroid cancer gene detection kit are loosely connected, which can easily cause solution overflow; some kits are easily stored in low temperatures, which can interfere with subsequent detection; lack of special syringe placement, making it inconvenient to operate.
A thyroid cancer gene detection kit is designed, using a PC-based kit and cover plate to realize liquid transmission between the solution bottle and the mixing bottle through the connecting rod and track structure, and precise docking is achieved using steering gears and directional dials. The filter plate filters soluble particles and the rubber ring remains sealed.
It realizes automatic and accurate injection of the solution, simplifies the operation process, prevents solution spillover and impurities from interfering with, and improves the reliability and repeatability of the kit.
Smart Images
Figure CN119913031A_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to the technical field of thyroid cancer gene detection, and in particular to a thyroid cancer gene detection kit and a method for using the kit. Background Art
[0002] Thyroid cancer gene testing is a testing technology that assesses an individual's risk of thyroid cancer, diagnoses thyroid cancer, guides treatment options, and predicts disease prognosis by testing genetic information in samples such as thyroid tissue or blood. It mainly focuses on genes in thyroid cells that are closely related to the occurrence and development of cancer.
[0003] The thyroid cancer gene detection kit is a tool kit specially designed for detecting thyroid cancer-related genes. It integrates various reagents, materials and containers required for gene testing to facilitate users to fuse samples with internal reagents, and obtain and analyze thyroid cancer-related gene information through mixed solvents through external medical equipment.
[0004] Currently, 1. Most of the existing test kits are disposable consumables, and the connections between their components are relatively loose. The reagent bottles are simply placed in the test kit and need to be injected one by one, which makes it very easy for the solution to overflow during the injection process; 2. Many existing test kits do not provide a dedicated placement for the injection needle when injecting blood samples and other operations; 3. Some of the solutions involved are prone to produce soluble particles during low-temperature storage, which causes interference when mixing with blood samples later. Summary of the invention
[0005] In view of the deficiencies of the prior art, the present invention provides a thyroid cancer gene detection kit and a method of using the same, which solves the problem proposed in the above background technology that the connections between the components are relatively loose and soluble particles are easily generated, resulting in interference when mixed with blood samples later.
[0006] To achieve the above objectives, the present invention is implemented through the following technical solutions: a thyroid cancer gene detection kit and a method of using the same, comprising a kit, one side of the kit is fitted with a cover plate and rotatably connected, both ends of the inner wall of the kit are fixedly connected to a first track, one side of the inner wall of the kit is fixedly connected to a second track below the first track, the first track and the second track are respectively slidably connected to a first beam and a second beam through sliders on their surfaces, one side of the first beam is connected to a base by bolts, one side of the base is fixedly connected to one end of an external frame, the other side of the external frame is rotatably connected to the second beam, and both ends of the outer wall of the base are connected to one end of a first connecting rod. The base is rotatably connected, and the other end of the first connecting rod is rotatably connected to one side of the cover plate through the second connecting rod. The inside of the base is fixedly connected with a mounting barrel, the top of the mounting barrel is slidably connected with a ring frame, the outer wall of the ring frame is fixedly connected with a solution bottle, the inside of the mounting barrel is sleeved with a mixing bottle, the top wall of the reagent box is rotatably connected with a steering gear, the top of the reagent box is fixedly connected with a directional dial, the cover plate is fixedly connected with a C-shaped frame on one side corresponding to the reagent box, a through hole for fixedly connecting a first pipe mouth sealing plug is penetrated through the surface of the solution bottle, a sealing cover is threadedly connected to the position of the solution bottle surface corresponding to the first pipe mouth sealing plug, and the first pipe mouth sealing plug is located inside the sealing cover.
[0007] A supporting and fixing gear is fixedly connected between the inner walls of the external frame, an idler wheel is rotatably connected above the supporting and fixing gear, the idler wheels are connected by a toothed belt sleeved on the outside, a transmission gear is rotatably connected between the inner walls of the external frame, the transmission gear is located above the toothed belt and meshed with the toothed belt, and a pry bar is rotatably connected to the outside of the external frame.
[0008] A toothed disc is fixedly connected to the top of the annular frame, and a first liquid discharge port is penetrated and opened on one side of the annular frame.
[0009] A one-way valve is fixedly connected to one side of the solution bottle, and a piston is movably connected to the other side of the solution bottle through a spring. A push rod is fixedly connected to the axis of the piston, and one end of the push rod passes through the outer wall of one side of the solution bottle.
[0010] The inner wall at the top of the mixing bottle is threadedly connected with a disassembly cover, the inner wall of the mixing bottle is fixedly connected with a filter plate, the interior of the mixing bottle is fixedly connected with a delivery pipe, one end of the delivery pipe passes through the filter plate and extends to the bottom thereof, the other end of the delivery pipe passes through the disassembly cover and extends to the top thereof, a second liquid discharge port is penetrated through one side of the outer wall of the mixing bottle, a plug column is fixedly connected to one side of the outer wall of the mixing bottle, and the second liquid discharge port is located between the plug columns.
[0011] Optionally, the test kit and the cover plate are made of PC material, and the top of the cover plate is fixedly connected with an elastic buckle, and the position of the top of the test kit corresponding to the elastic buckle is fixedly connected with a semicircular structure that engages with it, and at the same time, the test kit and the cover plate are fixedly connected with rubber strips that fit tightly with each other at the joints, and the cover plate forms an opening and closing structure on the outer wall of the test kit, and a folding structure is formed between the first connecting rod and the second connecting rod.
[0012] Optionally, the base and the external frame slide on the first track and the second track respectively through the first connecting rod and the second connecting rod and form a flip structure, and the mixing bottle is flipped 30° through the base. At the same time, the mixing bottle and the solution bottle are both made of glass, and the axis points of the solution bottles are distributed in a cross shape with equal angles about the annular frame. At the same time, the outer walls of the solution bottles are bonded with drug labels.
[0013] Optionally, a visual window is provided through one side of the mounting barrel, and a scale is marked on the outer wall of the mixing bottle corresponding to the position of the visual window, and a slot for inserting and connecting a plug column is provided on the inner wall of the mounting barrel, and a sliding groove for slidingly connecting a ring frame is provided on one side of the inner wall of the mounting barrel, and the ring frame and the mounting barrel are integrally connected.
[0014] Optionally, the solution bottle is provided with a through hole at a position corresponding to the first drain port, and the first drain port is consistent with the second drain port in position, and the first drain port and the second drain port are located above the filter plate, and at the same time, the outer wall of the mixing bottle is covered with a circular rubber ring at a position corresponding to the second drain port, and the inner wall of the annular frame is covered with an arc-shaped rubber ring at a position corresponding to the first drain port, and the arc-shaped rubber ring slides and fits under the circular rubber ring.
[0015] Optionally, the disassembly cover is threadedly connected to a structure consistent with the sealing cover, and a second pipe mouth sealing plug is provided at a position corresponding to the delivery pipe at the top of the disassembly cover, and the second pipe mouth sealing plug is an integrated structure consisting of two groups of inner and outer circular parts, the outer circular part of which is glued to the top of the disassembly cover, and the inner circular part of which is inserted into the inner wall of the pipe mouth end of the delivery pipe, and at the same time, a cross-shaped through-groove is provided on the surface of the first pipe mouth sealing plug and the second pipe mouth sealing plug.
[0016] Optionally, the steering gear is distributed at 30° inside the test kit, and the toothed disc is meshingly connected to the steering gear, and the annular frame is rotatably connected to the outer wall of the mixing bottle through the toothed disc and the steering gear, and at the same time, the shaft rod at the axis center of the steering gear is fixedly connected to the circular handle rotatably connected to the top of the directional dial, and the surface of the directional dial is marked with cross-shaped scale lines.
[0017] Optionally, the supporting fixed gear and the transmission gear are respectively located at the opposite ends of the bottom and top of the toothed belt, and the toothed belt constitutes a transmission structure, and the transmission gear constitutes relative motion with it, and at the same time, the transmission gear and the pry bar are coaxially connected, the pry bar is located between the cover plate and the base, and the pry bar constitutes a rotating structure through the transmission gear, and the top of the pry bar is in contact with the top rod.
[0018] Optionally, the outer wall of the solution bottle is covered with a rubber pad at a position corresponding to the push rod, and the piston is attached to and slidably connected to the inner wall of the solution bottle via the push rod, and the end of the piston adjacent to the annular frame is conical, and the other side is circular. At the same time, the piston forms an elastic reset structure through a spring, and the outer wall of the spring is sleeved with an elastic silicone sleeve.
[0019] A thyroid cancer gene detection kit for use in any one of the above claims and a method of using the kit, comprising the following specific steps: Step 1: The solution bottle is filled with a buffer bottle, dNTp, a primer bottle and Taq enzyme respectively. The kit is usually stored in a freezing device. During the test, the elastic buckle on the top of the cover is unfastened and the handle is pulled to open the cover. During this process, the second connecting rod rotates with the cover and stretches the first connecting rod. The first connecting rod pulls the base, and then drives the external frame, so that the first beam and the second beam slide up along the bottom notches of the first track and the second track respectively, to achieve structural angle flipping; Step 2: The base moves outward to contact the pry bar. When the base pushes the pry bar in the external frame, the transmission gear rotates under the drive of the toothed belt, driving the pry bar to rotate, and the top of the pry bar pushes the ejector rod, and the ejector rod pushes the piston to slide to one side of the one-way valve, and the solution flows into the mixing bottle through the first and second liquid discharge ports. The filter disc can filter soluble particles that are easy to exist in the solution. When a group of solutions is injected, the cover plate is pushed to reset, the first connecting rod and the second connecting rod are folded, and the base and the external frame slide back to the bottom along the track, the ejector rod is separated from the pry bar, and the piston is reset under the action of the spring; Step 3: After resetting, the mixing bottle is tilted at 30°, and the gear disc on the top of the ring frame is inserted through the tooth shape on the surface of the steering gear under the steering structure and meshed with it. The operator turns the directional dial handle through the visual window, and controls the steering gear to rotate according to the cross-shaped scale line and the pointer, ensuring that the first discharge port can accurately mate with the second discharge port on the surface of the solution bottle when the ring frame rotates. Repeat the above steps to open the cover plate to inject other groups of solutions. The range of the pry rod push rod can be adjusted by the cover plate opening angle to control the solution volume as needed. The operator can observe the injection volume of each solution through the visual window on the surface of the installation barrel and the scale on the surface of the mixing bottle; Step 4: After all solutions are injected, turn the sealing cover on the top of the disassembly cover, and inject the processed blood sample through the cross-shaped notch on the top of the second tube port sealing plug through the external injection needle. The blood sample enters the mixing bottle through the delivery tube and mixes with the previously injected solution. During this process, the operator can stably place the syringe on the C-frame; Step 5: When the solution reserve in the solution bottle is insufficient, the sealing cap can be unscrewed and the solution can be poured through the sealing plug of the first pipe mouth. The mixing bottle, as a reaction container, adopts a plug-in connection structure and can be disassembled for cleaning and disinfection before reuse. During installation, the plug posts on the mixing bottle are precisely docked with the corresponding structures on the inner wall of the mounting barrel to ensure that the second discharge port is accurately docked with the first discharge port, and at the same time, the scale on the surface of the mixing bottle is aligned with one side of the visible window of the mounting barrel.
[0020] The present invention provides a thyroid cancer gene detection kit and a method of using the kit, which has the following beneficial effects: The thyroid cancer gene detection kit and its use method, the kit and the cover are made of PC material to provide stable support for the whole and prevent deformation and damage. The first connecting rod and the second connecting rod drive the base and the external frame to move when the cover is opened and closed, so as to realize the liquid transmission function between the solution bottle and the mixing bottle, and realize that there is no need for manual injection of each solution one by one. Only by the simple operation of opening the cover, the transmission and linkage process can be triggered, so that the solution in the solution bottle is automatically and accurately injected into the mixing bottle through the discharge port, which greatly simplifies the operation process. At the same time, it can be compactly stored when closed, which not only makes full use of the internal space, but also improves the reliability and repeatability of the kit.
[0021] The thyroid cancer gene detection kit and the use method thereof can realize the precise docking of the first liquid discharge port and the second liquid discharge port through the meshing of the steering gear and the toothed disc and the precise control of the directional dial, combined with the specific distribution of the solution bottles on the annular frame, to ensure that the solution flows into the mixing bottle at a predetermined speed and sequence. At the same time, the transmission structure composed of the supporting fixed gear, the transmission gear and the toothed belt can accurately control the rotation of the pry rod, and then adjust the piston displacement by adjusting the push rod to control the solution injection amount.
[0022] The thyroid cancer gene detection kit and its use method, the filter disc can effectively intercept insoluble particles in the solution, prevent impurities from interfering with the key links of gene detection, and at the same time, the rubber ring components at each drainage port and the rubber pads at the corresponding push rod positions of the solution bottle can not only maintain a good sealing effect in dynamic operation to prevent solution leakage and cross contamination, but also reduce component wear, ensure the long-term stable operation of the kit, and maintain the purity of the solution and the stability of the detection environment.
[0023] The thyroid cancer gene detection kit and its use method are characterized in that the disassembly cover can be used to conveniently disassemble the mixing bottle for cleaning and disinfection; the first tube mouth sealing plug and the second tube mouth sealing plug can be used to respectively replenish the solution in the solution bottle, add the blood sample in the mixing bottle and extract the mixed solution, thereby simplifying the operation process and improving the work efficiency; in addition, the visual window of the installation barrel and the scale of the mixing bottle are convenient for real-time observation of the solution injection amount, thereby enhancing the practicality and flexibility of the kit.
[0024] The thyroid cancer gene detection kit and its use method, the device can be adapted to several groups of mixing bottles and flexibly replaced, effectively improving the detection efficiency, reducing the sample waiting time, and ensuring the continuity of the detection work. When it is necessary to add blood samples to the mixing bottle or replenish the solution bottle, the operator can stably place the syringe on the C-shaped frame for easy access at any time, avoiding the problems of slipping, contamination or inconvenience in finding caused by the syringe being placed arbitrarily on the operating table, and reducing the interference caused by improper placement of tools. BRIEF DESCRIPTION OF THE DRAWINGS
[0025] Figure 1 It is a schematic diagram of the main view structure of the invention; Figure 2 It is a schematic diagram of the main view closed structure in the invention; Figure 3 It is a schematic diagram of the internal structure of the kit in the invention; Figure 4 A schematic diagram of the distribution structure of the first connecting rod and the second connecting rod in the invention; Figure 5 It is a schematic diagram of the internal structure of the external frame in the invention; Figure 6 It is a schematic diagram of the base structure in the invention; Figure 7 It is a schematic diagram of the structure of the mixing bottle in the invention; Figure 8 It is a schematic diagram of the ring frame structure in the invention; Fig. 9 It is a schematic diagram of the structure of the solution bottle in the invention.
[0026] In the figure: 1, reagent box; 2, cover plate; 3, first track; 4, second track; 5, first beam; 6, second beam; 7, base; 8, external frame; 801, support fixed gear; 802, idler wheel; 803, toothed belt; 804, transmission gear; 805, pry bar; 9, first connecting rod; 10, second connecting rod; 11, installation barrel; 12, ring frame; 1201, toothed disc; 1202, first liquid discharge port; 13, solution bottle; 13 01. One-way valve; 1302. Spring; 1303. Piston; 1304. Push rod; 1305. Elastic silicone sleeve; 14. Mixing bottle; 1401. Disassembly cover; 1402. Filter plate; 1403. Delivery pipe; 1404. Second liquid discharge port; 1405; Plug column; 15. Steering gear; 16. Directional dial; 17. C-type frame; 18. First pipe opening sealing plug; 19. Sealing cover; 20. Elastic buckle; 21. Second pipe opening sealing plug. DETAILED DESCRIPTION
[0027] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.
[0028] In the description of the present invention, unless otherwise specified, "plurality" means two or more than two; the orientations or positional relationships indicated by the terms "upper", "lower", "left", "right", "inner", "outer", "front end", "rear end", "head", "tail", etc. are based on the orientations or positional relationships shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operate in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first", "second", "third", etc. are only used for descriptive purposes and cannot be understood as indicating or implying relative importance.
[0029] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "connected" and "connection" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.
[0030] See also Figures 1 to 9The present invention provides a technical solution: a thyroid cancer gene detection kit and a method for using the same, comprising a kit 1, one side of the kit 1 is fitted with a cover plate 2 and rotatably connected, both ends of the inner wall of the kit 1 are fixedly connected with a first track 3, one side of the inner wall of the kit 1 is fixedly connected with a second track 4 corresponding to the lower side of the first track 3, the first track 3 and the second track 4 are respectively slidably connected with a first beam 5 and a second beam 6 through sliders on their surfaces, one side of the first beam 5 is connected to a base 7 by bolts, one side of the base 7 is fixedly connected to one end of an external frame 8, the other side of the external frame 8 is rotatably connected to the second beam 6, the two ends of the outer wall of the base 7 are rotatably connected to one end of a first connecting rod 9, and the other end of the first connecting rod 9 The base 7 is rotatably connected to one side of the cover 2 through the second connecting rod 10, and a mounting barrel 11 is fixedly connected inside the base 7. A ring frame 12 is slidably connected to the top of the mounting barrel 11, and a solution bottle 13 is fixedly connected to the outer wall of the ring frame 12. A mixing bottle 14 is sleeved inside the mounting barrel 11, and a steering gear 15 is rotatably connected to the inner top wall of the reagent kit 1. A directional dial 16 is fixedly connected to the top of the reagent kit 1, and a C-shaped frame 17 is fixedly connected to the side of the cover 2 corresponding to the reagent kit 1. A through hole for fixedly connecting a first pipe mouth sealing plug 18 is opened through the surface of the solution bottle 13, and a sealing cover 19 is threadedly connected to the position of the first pipe mouth sealing plug 18 on the surface of the solution bottle 13 corresponding to the first pipe mouth sealing plug 18, and the first pipe mouth sealing plug 18 is located inside the sealing cover 19.
[0031] A supporting fixed gear 801 is fixedly connected between the inner walls of the external frame 8, an idler wheel 802 is rotatably connected above the supporting fixed gear 801, and the idler wheels 802 are connected to each other through a toothed belt 803 sleeved on the outside thereof. A transmission gear 804 is rotatably connected between the inner walls of the external frame 8, and the transmission gear 804 is located above the toothed belt 803 and meshed with it. A pry bar 805 is rotatably connected to the outside of the external frame 8.
[0032] A toothed disc 1201 is fixedly connected to the top of the annular frame 12 , and a first liquid discharge port 1202 is formed through one side of the annular frame 12 .
[0033] A one-way valve 1301 is fixedly connected to one side of the solution bottle 13, and a piston 1303 is movably connected to the other side of the solution bottle 13 through a spring 1302. A push rod 1304 is fixedly connected to the axis of the piston 1303, and one end of the push rod 1304 passes through the outer wall of one side of the solution bottle 13.
[0034] The inner wall at the top of the mixing bottle 14 is threadedly connected with a disassembly cover 1401, the inner wall of the mixing bottle 14 is fixedly connected with a filter plate 1402, the interior of the mixing bottle 14 is fixedly connected with a delivery pipe 1403, one end of the delivery pipe 1403 passes through the filter plate 1402 and extends to the bottom thereof, the other end of the delivery pipe 1403 passes through the disassembly cover 1401 and extends to the top thereof, a second liquid discharge port 1404 is penetrated on one side of the outer wall of the mixing bottle 14, a plug post 1405 is fixedly connected to one side of the outer wall of the mixing bottle 14, and the second liquid discharge port 1404 is located between the plug posts 1405.
[0035] In this embodiment, Figure 1 , Figure 2 and Figure 3 As shown, the reagent box 1 and the cover plate 2 are made of PC material, and an elastic buckle 20 is fixedly connected to the top of the cover plate 2, and a semicircular structure that is engaged with the elastic buckle 20 is fixedly connected to the position of the top of the reagent box 1 corresponding to the elastic buckle 20, and at the same time, the reagent box 1 and the cover plate 2 are fixedly connected with adhesive strips that are tightly fitted to each other, and the cover plate 2 forms an opening and closing structure on the outer wall of the reagent box 1, and a folding structure is formed between the first connecting rod 9 and the second connecting rod 10; the material of the reagent box 1 and the cover plate 2 can provide a stable support structure during frequent opening and closing operations, and placement under different environmental conditions, effectively preventing the reagent box 1 and the cover plate 2 from being deformed or damaged, thereby ensuring the reliability and repeatability of the entire detection process, and at the same time, the tightly fitting adhesive strip can further enhance the sealing when the cover plate 2 is closed, and when the cover plate 2 is opened, the first connecting rod 9 and the second connecting rod 10 can drive the base 7 and the external frame 8 to be unfolded, and when the cover plate 2 is closed, the first connecting rod 9 and the second connecting rod 10 fold back to restore the base 7 and the external frame 8 to their initial positions and be compactly stored inside the reagent box 1.
[0036] In this embodiment, Figure 1 , Figure 3 and Figure 4As shown, the base 7 and the external frame 8 slide on the first track 3 and the second track 4 respectively through the first connecting rod 9 and the second connecting rod 10 and present a flip structure, and the mixing bottle 14 is flipped at 30° through the base 7, and the mixing bottle 14 and the solution bottle 13 are both made of glass, and the axis points of the solution bottles 13 about the annular frame 12 are cross-shaped and equiangularly distributed, and the outer walls of the solution bottles 13 are bonded and connected with drug labels; the flip structure effectively utilizes the space inside the reagent box 1, and realizes the complex liquid transmission and mixing functions between the solution bottle 13 and the mixing bottle 14 without occupying too much additional space. When the reagent box 1 is in a closed state, the various components can be compactly stored together, and when it is opened for use, it can be unfolded through the linkage between the various components, providing sufficient operating space for the processing and reaction of the solution, improving the utilization rate and functional integration of the internal space of the reagent box 1, and clearly marking the name of the reagent in each solution bottle 13 and other information, so that the operator can quickly identify and select the required reagent during the operation, and avoid the solution mixing error caused by misoperation.
[0037] In this embodiment, Figure 1 , Figure 7 and Figure 8 As shown, a visual window is provided on one side of the installation barrel 11, and a scale is engraved on the outer wall of the mixing bottle 14 at the position corresponding to the visual window, and a notch for plugging and connecting the plug post 1405 is provided on the inner wall of the installation barrel 11, and a slide groove for slidingly connecting the annular frame 12 is provided on one side of the inner wall of the installation barrel 11, and the annular frame 12 is integrally connected with the installation barrel 11; in operation, a series of mechanical transmissions are triggered by pulling open the cover plate 2, and when the pry bar 805 contacts the push rod 1304 and pushes the piston 1303 to squeeze the solvent in the solution bottle 13 into the mixing bottle 14, the visual window of the installation barrel 11 and the scale on the outer wall of the mixing bottle 14 play an important role, and the operator can observe the volume of the solution flowing into the mixing bottle 14 in real time and intuitively, and accurately know the volume of each solution according to the scale. The specific amount of liquid injected can be controlled by controlling the opening angle of the cover 2 to change the distance that the pry bar 805 squeezes the push rod 1304 when it rotates, thereby indirectly regulating the stroke of the piston 1303 and the discharge amount of the solution, so that each reagent is added in strict accordance with the precise ratio required for thyroid cancer gene detection. The plug-in connection structure between the plug column 1405 and the groove on the inner wall of the mounting barrel 11 provides a precise positioning function for the re-installation of the mixing bottle 14 after disassembly. In actual applications, the mixing bottle 14 needs to be cleaned and disinfected and is disassembled. When it is reinstalled, relying on this unique plug-in structure, the mixing bottle 14 can accurately return to the initially designed position to ensure that the second liquid discharge port 1404 on its outer wall is effectively and accurately connected with the corresponding first liquid discharge port 1202 on the annular frame 12.
[0038] In this embodiment, Figure 7 and Figure 8 As shown, the solution bottle 13 is provided with a through hole corresponding to the first drain port 1202, and the first drain port 1202 is in the same position as the second drain port 1404, and the first drain port 1202 and the second drain port 1404 are located above the filter disc 1402. At the same time, the outer wall of the mixing bottle 14 is covered with a circular rubber ring at the position corresponding to the second drain port 1404, and the inner wall of the annular frame 12 is covered with an arc-shaped rubber ring at the position corresponding to the first drain port 1202, and the arc-shaped rubber ring slides and fits under the circular rubber ring; the filter disc 1402 can effectively intercept the insoluble substances that are easy to exist in the solution. Particles, thereby purifying the solution, preventing these impurities from entering the subsequent reaction system, and avoiding interference with key links in the gene detection process such as enzyme activity, primer and template binding, etc., taking into account the rotation of the annular frame 12 relative to the mixing bottle 14 during operation, and the sliding fit between the rubber rings can always maintain a good sealing effect during dynamic operation. At the same time, the rubber material itself has certain elasticity and adaptability. Even if there is a certain amount of wear during long-term use, it can compensate for the sealing gap through its own elastic deformation and continuously maintain good sealing performance.
[0039] In this embodiment, Figure 7 and Fig. 9 As shown, the disassembly cover 1401 is threadedly connected with a structure consistent with the sealing cover 19, and a second pipe mouth sealing plug 21 is provided at the position of the top of the disassembly cover 1401 corresponding to the delivery tube 1403, and the second pipe mouth sealing plug 21 is an integrated structure composed of two sets of inner and outer circular parts, the outer circular part of which is glued and connected to the top of the disassembly cover 1401, and the inner circular part is inserted into the inner wall of the pipe mouth end of the delivery tube 1403, and at the same time, the surfaces of the first pipe mouth sealing plug 18 and the second pipe mouth sealing plug 21 are provided with a cross-shaped through groove; the mixing bottle 14 will add blood samples, and cannot be effectively cleaned, which will not only affect the accuracy of the next test, but also effectively disinfect the inside of the mixing bottle 14. The first tube mouth sealing plug 18 can be used to supplement the solution required for daily use in the solution bottle 13 with the help of an external injection device, ensuring that there is always sufficient and appropriate amount of reagent in the solution bottle 13 for subsequent detection processes. The second tube mouth sealing plug 21 cooperates with the external injection device, and can be used to add blood samples to the mixing bottle 14, and after the blood sample and the solution are mixed, the mixed liquid can be extracted through the mixing bottle for detection. This multifunctional design greatly simplifies the operating process and improves work efficiency. It should be noted that in order to expand the detection throughput to meet the needs of large-scale genetic testing screening or diversified testing projects carried out in parallel, the device can be adapted to several groups of mixing bottles 14, which can be flexibly replaced to ensure the continuity of the detection work.
[0040] In this embodiment, Figure 3 and Figure 8As shown, the steering gear 15 is distributed at 30° inside the reagent box 1, and the toothed disc 1201 is meshed with the steering gear 15, and the annular frame 12 is rotatably connected to the outer wall of the mixing bottle 14 through the toothed disc 1201 and the steering gear 15, and at the same time, the shaft at the axis of the steering gear 15 is fixedly connected to the circular handle rotatably connected to the top of the directional dial 16, and the surface of the directional dial 16 is marked with cross-shaped scale lines; the meshing connection between the steering gear 15 and the toothed disc 1201 and the linkage structure with the directional dial 16 constitute a relatively stable rotation structure, combined with the 90° equiangular distribution of the solution bottles 13 on the annular frame 12 and the cross scale lines of the directional dial 16 for operation, it can achieve effective docking of the first discharge port 1202 and the second discharge port 1404, thereby ensuring that the solution can flow into the mixing bottle 14 at a predetermined speed and sequence.
[0041] In this embodiment, Figure 5 As shown, the supporting fixed gear 801 and the transmission gear 804 are respectively located at the opposite ends of the bottom and top of the toothed belt 803, and the toothed belt 803 constitutes a transmission structure, and the transmission gear 804 forms a relative motion with it, and at the same time, the transmission gear 804 is coaxially connected with the pry bar 805, the pry bar 805 is located between the cover plate 2 and the base 7, and the pry bar 805 forms a rotating structure through the transmission gear 804, and the top of the pry bar 805 is in contact with the top rod 1304; when the cover plate 2 is opened, the base 7 moves outward and contacts the pry bar 805, and the rotation of the pry bar 805 makes the top of the pry bar 805 fit with the top rod 1304, and pushes the top rod 1 304 moves, and the push rod 1304 further pushes the piston 1303 to slide toward the side of the one-way valve 1301, so that the solution in the solution bottle 13 is accurately injected into the mixing bottle 14 through the first discharge port 1202 and the second discharge port 1404. The transmission structure composed of the toothed belt 803 and the transmission gear 804 can accurately control the rotation angle and force of the pry bar 805, and then accurately control the displacement of the push rod 1304. The supporting fixed gear 801 is tightly engaged with the toothed belt 803. When the transmission gear 804 starts to rotate, the toothed belt 803 relies on the friction between the supporting fixed gear 801 to achieve effective transmission.
[0042] In this embodiment, Fig. 9As shown, the outer wall of the solution bottle 13 corresponding to the position of the push rod 1304 is covered with a rubber pad, and the piston 1303 is attached to and slidably connected to the inner wall of the solution bottle 13 through the push rod 1304, and the end of the piston 1303 adjacent to the annular frame 12 is conical, and the other side is circular. At the same time, the piston 1303 forms an elastic reset structure through the spring 1302, and the outer wall of the spring 1302 is sleeved with an elastic silicone sleeve 1305; the rubber on the outer wall of the push rod 1304 can improve the sealing performance, thereby ensuring that the internal solution is not polluted by the outside and can be stored and used normally, the tapered part of the piston 1303 can guide the solution to flow toward the discharge port, and improve the discharge efficiency of the solution. After the solution is injected, the spring 1302 can automatically reset the piston 1303 to the initial position, and the elastic silicone sleeve 1305 has a certain degree of stretchability, which can be used as an isolation layer to separate the spring 1302 from the solution to ensure the purity of the solution.
[0043] In this embodiment, Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figure 5 , Figure 6 , Figure 7 , Figure 8 and Fig. 9 As shown, the above-mentioned thyroid cancer gene detection kit and its use method are used, and the specific steps are as follows: Step 1: The solution bottle 13 is respectively filled with a buffer bottle, dNTp, a primer bottle and Taq enzyme. The test kit 1 is usually stored in a freezing device. When testing, the elastic buckle 20 on the top of the cover plate 2 is unlocked, and the handle is pulled to open the cover plate 2. During this process, the second connecting rod 10 rotates with the cover plate 2 and stretches the first connecting rod 9. The first connecting rod 9 pulls the base 7, and then drives the external frame 8, so that the first crossbeam 5 and the second crossbeam 6 slide up along the bottom notches of the first track 3 and the second track 4 respectively, so as to realize the structural angle flip; Step 2: the base 7 moves outward to contact the pry bar 805. When the base 7 pushes the pry bar 805 in the external frame 8, the transmission gear 804 rotates under the transmission of the toothed belt 803, driving the pry bar 805 to rotate, and the top of the pry bar pushes the ejector rod 1304. The ejector rod 1304 pushes the piston 1303 to slide toward the side of the one-way valve 1301, and the solution flows into the mixing bottle 14 through the first liquid discharge port 1202 and the second liquid discharge port 1404. The filter disc 1402 can filter the soluble particles that are easy to exist in the solution. When a group of solution is injected, the cover plate 2 is pushed to reset, the first connecting rod 9 and the second connecting rod 10 are folded, the base 7 and the external frame 8 slide back to the bottom along the track, the ejector rod 1304 is separated from the pry bar 805, and the piston 1303 is reset under the action of the spring 1302; Step 3: After resetting, the mixing bottle 14 is tilted at 30°, and the toothed disc 1201 on the top of the annular frame 12 is inserted through the toothed shape on the surface of the steering gear 15 under the steering structure and is meshed with it. The operator turns the handle of the directional dial 16 through the visual window, and controls the rotation of the steering gear 15 according to the cross-shaped scale line and the pointer, ensuring that the first discharge port 1202 can accurately dock with the second discharge port 1404 on the surface of the solution bottle 13 when the annular frame 12 rotates. Repeat the above steps of opening the cover plate 2 to inject other groups of solutions. The range of the push rod 1304 of the pry bar 805 can be adjusted by adjusting the opening angle of the cover plate 2 according to needs to control the amount of solution. The operator can observe the injection amount of each solution through the visual window on the surface of the mounting barrel 11 and the scale on the surface of the mixing bottle 14; Step 4: After all solutions are injected, the sealing cover 19 on the top of the disassembly cover 1401 is rotated, and the processed blood sample is injected through the cross-shaped notch on the top of the second tube opening sealing plug 21 through the external injection needle tube. The blood sample enters the mixing bottle 14 through the delivery tube 1403 and mixes with the previously injected solution. During this process, the operator can stably place the syringe on the C-shaped frame 17; Step 5: When the solution reserve in the solution bottle 13 is insufficient, the sealing cap 19 can be unscrewed and the solution can be poured through the first pipe mouth sealing plug 18. The mixing bottle 14 is used as a reaction container and adopts a plug-in connection structure. It can be disassembled for cleaning and disinfection before reuse. During installation, the plug post 1405 on the mixing bottle 14 is precisely docked with the corresponding structure on the inner wall of the mounting barrel 11 to ensure that the second liquid discharge port 1404 is accurately docked with the first liquid discharge port 1202, and at the same time, the scale on the surface of the mixing bottle 14 is aligned with one side of the visible window of the mounting barrel 11.
[0044] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.
Claims
1. A thyroid cancer gene detection kit and a method for using the kit, comprising the kit (1), characterized in that: One side of the reagent box (1) is fitted with and rotatably connected to the cover plate (2); both ends of the inner wall of the reagent box (1) are fixedly connected to a first track (3); one side of the inner wall of the reagent box (1) is fixedly connected to a second track (4) corresponding to the lower side of the first track (3); the first track (3) and the second track (4) are respectively slidably connected to a first crossbeam (5) and a second crossbeam (6) via sliders on their surfaces; one side of the first crossbeam (5) is connected to a base (7) via bolts; one side of the base (7) is fixedly connected to one end of an external frame (8); the other side of the external frame (8) is rotatably connected to the second crossbeam (6); both ends of the outer wall of the base (7) are rotatably connected to one end of a first connecting rod (9); the other end of the first connecting rod (9) is rotatably connected to one side of the cover plate (2) via a second connecting rod (10); The base (7) is fixedly connected to a mounting barrel (11) inside, the top of the mounting barrel (11) is slidably connected to an annular frame (12), the outer wall of the annular frame (12) is fixedly connected to a solution bottle (13), the mounting barrel (11) is sleeved with a mixing bottle (14) inside, the inner top wall of the reagent box (1) is rotatably connected to a steering gear (15), the top of the reagent box (1) is fixedly connected to a directional dial (16), the cover plate (2) is fixedly connected to a C-shaped frame (17) on a side corresponding to the reagent box (1), a through hole for fixedly connecting a first pipe opening sealing plug (18) is formed through the surface of the solution bottle (13), a sealing cover (19) is threadedly connected to a position on the surface of the solution bottle (13) corresponding to the first pipe opening sealing plug (18), and the first pipe opening sealing plug (18) is located inside the sealing cover (19); A supporting fixed gear (801) is fixedly connected between the inner walls of the external frame (8); an idler wheel (802) is rotatably connected above the supporting fixed gear (801); the idler wheels (802) are connected to each other via a toothed belt (803) sleeved on the outside thereof; a transmission gear (804) is rotatably connected between the inner walls of the external frame (8); the transmission gear (804) is located above the toothed belt (803) and meshed with the toothed belt; and a pry bar (805) is rotatably connected to the outside of the external frame (8); A toothed disc (1201) is fixedly connected to the top of the annular frame (12), and a first liquid discharge port (1202) is provided through one side of the annular frame (12); A one-way valve (1301) is fixedly connected to one side of the solution bottle (13), and a piston (1303) is movably connected to the other side of the solution bottle (13) via a spring (1302). A push rod (1304) is fixedly connected to the axis of the piston (1303), and one end of the push rod (1304) passes through the outer wall of one side of the solution bottle (13); The inner wall at the top of the mixing bottle (14) is threadedly connected to a disassembly cover (1401), the inner wall of the mixing bottle (14) is fixedly connected to a filter plate (1402), the interior of the mixing bottle (14) is fixedly connected to a delivery pipe (1403), one end of the delivery pipe (1403) passes through the filter plate (1402) and extends to the bottom thereof, the other end of the delivery pipe (1403) passes through the disassembly cover (1401) and extends to the top thereof, a second liquid discharge port (1404) is formed through one side of the outer wall of the mixing bottle (14), a plug post (1405) is fixedly connected to one side of the outer wall of the mixing bottle (14), and the second liquid discharge port (1404) is located between the plug posts (1405).
2. A thyroid cancer gene detection kit and a method for using the same according to claim 1, characterized in that: The reagent box (1) and the cover plate (2) are made of PC material, and the top of the cover plate (2) is fixedly connected with an elastic buckle (20), and a semicircular structure that engages with the elastic buckle (20) is fixedly connected to the top of the reagent box (1), and at the position corresponding to the elastic buckle (20), a rubber strip that fits tightly with each other is fixedly connected to the joint position of the reagent box (1) and the cover plate (2), and the cover plate (2) forms an opening and closing structure on the outer wall of the reagent box (1), and a folding structure is formed between the first connecting rod (9) and the second connecting rod (10).
3. A thyroid cancer gene detection kit and its use method according to claim 1, characterized in that: The base (7) and the external frame (8) slide on the first track (3) and the second track (4) respectively via the first connecting rod (9) and the second connecting rod (10) and are in a flipping structure, and the mixing bottle (14) flips at 30 degrees via the base (7), and the mixing bottle (14) and the solution bottle (13) are both made of glass, and the axis points of the solution bottles (13) are equiangularly distributed in a cross shape with respect to the annular frame (12), and the outer walls of the solution bottles (13) are bonded and connected with drug labels.
4. A thyroid cancer gene detection kit and a method of using the same according to claim 1, characterized in that: A visual window is provided through one side of the installation barrel (11), and a scale is engraved on the outer wall of the mixing bottle (14) at a position corresponding to the visual window. A notch for inserting and connecting the plug post (1405) is provided on the inner wall of the installation barrel (11), and a sliding groove for slidingly connecting the annular frame (12) is provided on one side of the inner wall of the installation barrel (11), and the annular frame (12) and the installation barrel (11) are connected in an integrated manner.
5. A thyroid cancer gene detection kit and its use method according to claim 1, characterized in that: The solution bottle (13) is provided with a through hole at a position corresponding to the first liquid discharge port (1202), and the first liquid discharge port (1202) and the second liquid discharge port (1404) are at the same position, and the first liquid discharge port (1202) and the second liquid discharge port (1404) are located above the filter plate (1402). At the same time, the outer wall of the mixing bottle (14) at a position corresponding to the second liquid discharge port (1404) is covered with a circular rubber ring, and the inner wall of the annular frame (12) at a position corresponding to the first liquid discharge port (1202) is covered with an arc-shaped rubber ring, and the arc-shaped rubber ring slides and fits below the circular rubber ring.
6. A thyroid cancer gene detection kit and a method of using the same according to claim 1, characterized in that: The disassembly cover (1401) is threadedly connected to a structure consistent with the sealing cover (19), and a second pipe opening sealing plug (21) is provided at a position on the top of the disassembly cover (1401) corresponding to the delivery pipe (1403), and the second pipe opening sealing plug (21) is an integrated structure consisting of two sets of inner and outer circular parts, wherein the outer circular part is glued to the top of the disassembly cover (1401), and the inner circular part is inserted into the inner wall of the pipe opening end of the delivery pipe (1403), and at the same time, the surfaces of the first pipe opening sealing plug (18) and the second pipe opening sealing plug (21) are provided with a cross-shaped through-going groove.
7. A thyroid cancer gene detection kit and a method of using the same according to claim 1, characterized in that: The steering gear (15) is distributed inside the reagent box (1) at an angle of 30 degrees, and the toothed disc (1201) is meshedly connected to the steering gear (15), and the annular frame (12) is rotatably connected to the outer wall of the mixing bottle (14) through the toothed disc (1201) and the steering gear (15), and at the same time, the shaft at the axis center of the steering gear (15) is fixedly connected to a circular handle rotatably connected to the top of the directional dial (16), and the surface of the directional dial (16) is marked with cross-shaped scale lines.
8. A thyroid cancer gene detection kit and its use method according to claim 1, characterized in that: The supporting fixed gear (801) and the transmission gear (804) are respectively located at opposite ends of the bottom and top of the toothed belt (803), and the toothed belt (803) forms a transmission structure, and the transmission gear (804) forms relative motion with the toothed belt (803), and the transmission gear (804) and the pry bar (805) are coaxially connected, and the pry bar (805) is located between the cover plate (2) and the base (7), and the pry bar (805) forms a rotating structure through the transmission gear (804), and the top of the pry bar (805) is in contact with the top rod (1304).
9. A thyroid cancer gene detection kit and a method of using the same according to claim 1, characterized in that: The position of the outer wall of the solution bottle (13) corresponding to the push rod (1304) is covered with a rubber pad, and the piston (1303) is attached to and slidably connected to the inner wall of the solution bottle (13) through the push rod (1304), and the end of the piston (1303) adjacent to the annular frame (12) is conical, and the other end is circular, and the piston (1303) forms an elastic reset structure through the spring (1302), and the outer wall of the spring (1302) is sleeved with an elastic silicone sleeve (1305).
10. A thyroid cancer gene detection kit for use in any one of claims 1 to 9 and a method of using the kit, characterized in that: The specific steps include: Step 1: a buffer bottle, dNTP, a primer bottle and Taq enzyme are respectively injected into the solution bottle (13), and the test kit (1) is usually stored in a freezing device. When testing, the elastic buckle (20) on the top of the cover plate (2) is unlocked, and the handle is pulled to open the cover plate (2). During this process, the second connecting rod (10) rotates with the cover plate (2) and stretches the first connecting rod (9). The first connecting rod (9) pulls the base (7), thereby driving the external frame (8), so that the first crossbeam (5) and the second crossbeam (6) slide up along the bottom notches of the first track (3) and the second track (4), respectively, to achieve structural angle flipping; Step 2: The base (7) moves outward to contact the pry bar (805). When the base (7) pushes the pry bar (805) in the external frame (8), the transmission gear (804) rotates under the transmission of the toothed belt (803), driving the pry bar (805) to rotate, and the top of the pry bar pushes the push rod (1304). The push rod (1304) pushes the piston (1303) to slide toward the side of the one-way valve (1301), and the solution flows into the mixing bottle (14) through the first liquid discharge port (1202) and the second liquid discharge port (1404). The filter plate (1402) can filter soluble particles that are easily present in the solution. When a group of solutions is injected, the cover plate (2) is pushed to reset, the first connecting rod (9) and the second connecting rod (10) are folded, the base (7) and the external frame (8) slide back to the bottom along the track, the push rod (1304) is separated from the pry bar (805), and the piston (1303) is reset under the action of the spring (1302); Step 3: After resetting, the mixing bottle (14) is tilted at 30°, and the toothed disc (1201) on the top of the annular frame (12) is inserted into the toothed surface of the steering gear (15) under the steering structure and meshed with it. The operator rotates the handle of the directional dial (16) through the visual window, and controls the rotation of the steering gear (15) according to the cross-shaped scale line and the pointer, ensuring that the first discharge port (1202) can accurately align with the second discharge port (1404) on the surface of the solution bottle (13) when the annular frame (12) rotates. Repeat the above-mentioned step of opening the cover plate (2) to inject other groups of solutions. The range of the pry bar (805) and the push rod (1304) can be adjusted according to the opening angle of the cover plate (2) to control the amount of solution. The operator can observe the injection amount of each solution through the visual window on the surface of the installation barrel (11) and the scale on the surface of the mixing bottle (14); Step 4: After all solutions are injected, the sealing cover (19) on the top of the disassembly cover (1401) is rotated, and the processed blood sample is injected through the cross-shaped notch on the top of the second tube opening sealing plug (21) through the external injection needle tube. The blood sample enters the mixing bottle (14) through the delivery tube (1403) and mixes with the previously injected solution. During this process, the operator can stably place the syringe on the C-shaped frame (17); Step 5: When the solution reserve in the solution bottle (13) is insufficient, the sealing cap (19) can be unscrewed and the solution can be poured through the first pipe opening sealing plug (18). The mixing bottle (14) is used as a reaction container and adopts a plug-in connection structure. It can be disassembled for cleaning and disinfection before reuse. During installation, the plug post (1405) on the mixing bottle (14) is precisely docked with the corresponding structure on the inner wall of the installation barrel (11) to ensure that the second liquid discharge port (1404) is accurately docked with the first liquid discharge port (1202), and at the same time, the scale on the surface of the mixing bottle (14) is aligned with one side of the visible window of the installation barrel (11).