Method for enhancing microbial gasification coal by using biomass nutrition additive

Abstract

The invention discloses a method for enhancing microbial gasification of coal by utilizing a biomass nutrition additive, which comprises the following steps: respectively crushing a coal sample and the biomass nutrition additive by utilizing a crusher, and stirring and mixing coal powder and biomass powder in a stirrer to obtain a reaction substrate; loading reaction substrates into fermentation bottles, loading an inorganic salt culture medium and bacterial liquid into the fermentation bottles, loading a vitamin solution and a trace metal solution into each fermentation bottle, adjusting the pH value to 7.0, sealing, carrying out fermentation culture in a 36-DEG C constant-temperature fermentation box, carrying out parallel tests on three groups, and respectively changing the reaction substrates into pure coal powder and pure biomass powder in a control group; according to reaction time, gas generated by reaction is extracted every three days at the beginning of the reaction, gas components in a fermentation system are detected by utilizing a gas chromatograph, air pressure in a bottle is detected by utilizing an air pressure sensor, the method is suitable for any environment, an oxygen-free environment is manufactured by adopting carbon dioxide exhausted by industrial production, utilization of carbon dioxide is realized, and the production cost is reduced. And the greenhouse effect is reduced.

Description

Technical Field

[0001] The invention relates to the technical field of fluidized mining, and in particular to a method for strengthening microbial gasification of coal by utilizing biomass nutrient additives. Background Art

[0002] Traditional coal mining technology usually produces coal pillars left in the goaf, and the geological conditions in some areas are complex, which makes it difficult to mine some coal resources according to the existing traditional mining technology. The underground legacy coal resources account for about 30% of the total resources and are resources that cannot be mined again. Fluidized mining is one of the important components of green coal mining technology. In fluidized mining, coal microbial gasification provides a new technical approach for deep coal resources, thin coal seams and legacy coal mining. If microorganisms can be injected into abandoned mines for in-situ coal gasification, this part of the residual coal can be mined in the form of methane, realizing microbial gasification coal mining. In addition, it has become a consensus in the industry that there is a reduction pathway for carbon dioxide in the formation of biomethane in coal seams. Therefore, carbon dioxide can be injected into coal seams to enhance methane production while providing an anaerobic environment for the survival of methanogens and making one-time use of carbon dioxide to achieve greenhouse gas emission reduction.

[0003] Coalbed methane is commonly known as "gas". Its main component is methane and coal, which is stored in the coal seam in an adsorbed state. At present, microbial coal gasification faces problems such as low gasification efficiency and high gasification cost. Although microorganisms can be used to achieve fluidized coal mining, the efficiency of microorganisms in converting coal into methane is low, and the methane production does not match the needs of industrial applications. One of the reasons is that the high carbon element composition of coal itself limits the use of microorganisms. Industry scholars have proposed to improve the methane conversion rate of coal by adding nutrient organic matter for co-fermentation to increase the nitrogen content in the reaction system.

[0004] The carbon-nitrogen ratio in the anaerobic fermentation system is also a factor that needs to be considered. Too high or too low a carbon-nitrogen ratio will inhibit the fermentation process. For example, algae formed by urban organic waste and eutrophication of water bodies should be properly handled in a timely manner, otherwise they will pose a serious threat to the environment, such as releasing odors, breeding various pests, spreading animal pathogens, and polluting surface and groundwater. Compared with other organic waste treatment methods, anaerobic fermentation can not only effectively reduce and remove a large amount of organic matter in the waste, but also produce biogas mainly composed of methane (60%) and carbon dioxide (40%), which can be directly used or converted into heat and electricity to meet local energy needs. Excessive nitrogen content in urban organic waste is usually an important reason for the failure of anaerobic fermentation. Excessive nitrogen will produce mild ammonia toxicity during anaerobic fermentation, inhibiting the occurrence of the reaction. At the same time, when coal is subjected to anaerobic fermentation alone, the excessive proportion of carbon elements may also lead to acidification inhibition of the reaction process.

[0005] Chinese patent CN 111075415 A discloses a low-energy consumption, zero-carbon emission recovery and carbon cycle method for fossil energy. The natural system under the stratum such as oil fields, coal mines, or gas reservoirs is used as a chemical reaction system. Low-value organic matter is fermented with composite original microorganisms of fossil energy. The obtained fermentation liquid, composite original microbial bacterial liquid, ultra-clean absorption liquid of combustion tail gas obtained by composite original microorganisms of fossil energy in a microbial electrosynthetic atmosphere, and / or carbon dioxide from other sources are continuously injected into the chemical reaction system by the absorption liquid of the original composite microorganisms, and free electrons e are introduced into the chemical reaction system. At the same time, artificial mass transfer is carried out through all well networks connected to the oil / ore / gas reservoir, such as production wells, water injection wells, and gas injection wells, with incubation as the main method and elastic drive of natural energy for oil and gas production. The present invention is applicable to all oil, gas reservoirs and underground coal mines that are being mined, mined out, and have been found to be unmined. However, considering that the existing fossil energy mining technology generally has the problems of high energy consumption, low efficiency and unsustainable resource utilization, traditional mining methods, such as thermal mining, water injection and gas injection, rely on a large amount of external energy input (such as heat energy, mechanical energy and chemical substances), resulting in large energy consumption and low mining efficiency, especially in the later development of oil fields and gas reservoirs, the extraction efficiency of resources is greatly reduced. In addition, the existing technology lacks deep application in the regulation of underground resources, especially in the mined or difficult to mine oil and gas reservoirs, the traditional method cannot effectively recover the remaining resources, which promotes the depletion of resources. In terms of microbial application, although microorganisms have potential in methane generation and carbon cycle, their role in energy mining systems has not been fully explored, especially the application of microbial electrosynthesis of atmosphere and composite original microorganisms is still limited, and efficient energy recovery and resource recovery cannot be achieved. These problems limit the sustainability and energy utilization efficiency of the existing technology, and the existing technology appears to be slightly insufficient.

[0006] Chinese patent CN 110678539 A discloses microbial conversion of CO2 and other C1 substrates into vegan nutrients, fertilizers, biostimulants, and a system for accelerating soil carbon sequestration, and provides microorganisms and biological processes for converting gaseous substrates such as renewable H2 and waste CO2 producer gas or synthesis gas into high-protein biomass, wherein the high-protein biomass can be used directly for human nutrition, or as a nutrient for plants, fungi or other microorganisms, or as a source of soil carbon, nitrogen and other mineral nutrients. The renewable H2 used in the process described herein can be produced by electrolysis using solar or wind energy, and the producer gas used in the process described herein can be derived from sources including: gasification of waste raw materials and / or biomass residues, waste gas from industrial processes, or natural gas, biogas or landfill gas. However, considering that the prior art has proposed innovative ideas in the process of converting CO2 into high-protein biomass, nutrients and fertilizers, it still faces problems such as low conversion efficiency, high energy demand, unstable raw material supply, complex process control, and poor environmental sustainability. Existing technologies rely on low-concentration gas sources such as waste CO2 and biomass residues. The conversion process is limited by the adaptability and conversion rate of microbial species, and large-scale applications face challenges in terms of economy and environmental pollution risks. Therefore, future technologies should focus on improving conversion efficiency, optimizing energy utilization, reducing costs, improving process controllability, and ensuring environmental sustainability to promote the commercial application of this technology. Existing technologies appear to be slightly insufficient.

[0007] As biomass, compared with chemical synthetic urea, peptone and other nitrogen-containing organic matter, urban organic waste and aquatic algae are more economical in price, can be obtained sustainably by collecting urban waste and planting algae, and their utilization is more environmentally friendly. The ideal carbon-nitrogen ratio of biomass nutrient stimulants and coal for microbial anaerobic fermentation is 20-30. For coal from different regions, reaction substrates with suitable carbon-nitrogen ratios can be obtained by flexibly combining them with these organic matter. Summary of the invention

[0008] Technical issues solved:

[0009] The purpose of the present invention is to overcome the technical problems of low gasification efficiency and high gasification cost faced by microbial coal gasification in the prior art. The present application provides a method for strengthening microbial gasification of coal using biomass nutrient additives, utilizing a variety of existing organic wastes to enhance the gas production efficiency of microbial coal gasification in experiments and improve coal utilization.

[0010] Technical solution:

[0011] To achieve the above objectives, this application is implemented through the following technical solutions:

[0012] A method for enhancing microbial gasification of coal using biomass nutrient additives comprises the following steps:

[0013] Step 1: The coal sample and the biomass nutrient additive are crushed by a crusher respectively, and after crushing, the coal powder and the biomass powder are obtained by passing through a 200-mesh sieve;

[0014] Step 2: Take coal powder and biomass powder at a carbon-nitrogen ratio of 20-30;

[0015] Step 3: Mix the coal powder and biomass powder in a mixer at 50-150 rpm for 3 minutes to obtain a reaction substrate;

[0016] Step 4: subject the fermentation bottle and the anaerobic fermentation box to high temperature and high pressure treatment at 126°C and 2.59 MPa for 15 minutes;

[0017] Step 5: 10 g of the reaction substrate was taken into a 250 ml fermentation bottle according to the mass volume ratio, 50 ml of inorganic salt culture medium and 50 ml of bacterial solution were taken into the fermentation bottle, and each fermentation bottle was then filled with 1 ml of vitamin solution and 1 ml of trace metal solution and the pH was adjusted to 7.0. After sealing, the fermentation was carried out in a 36 ° C constant temperature fermentation box. Three groups were tested in parallel. The reaction substrates of the control group were changed to 10 g of pure coal powder and 10 g of pure biomass powder respectively;

[0018] Step six: According to the reaction time, extract the gas produced by the reaction every 3 days after the reaction starts, use a gas chromatograph to detect the components of the gas in the fermentation system, use a pressure sensor to detect the air pressure in the bottle, and use the gas balance equation pV=nRT to calculate the total volume of the gas in the bottle based on the detected air pressure in the bottle. Use a gas chromatograph to detect the component volume of methane in the gas, and then use the change in gas production volume and time to calculate the methane production efficiency. The monitoring period is 30 days.

[0019] Preferably, the coal sample in step 1 is derived from the remaining coal resources in the target area for microbial gasification.

[0020] Preferably, the coal sample comes from Baiyinhua Coal Mine in Inner Mongolia, Madiliang Coal Mine in Inner Mongolia, Xiezhuang Coal Mine in Shandong or Lingxin Coal Mine in Ningxia.

[0021] Preferably, the biomass nutrient additive in step 1 is nitrogen-containing organic matter.

[0022] Preferably, the nitrogen-containing organic matter is one or more of municipal sludge, animal feces, algae, and kitchen waste.

[0023] Preferably, the algae is one or more of Dulse, Nannochloropsis, Spirulina, and Chlorella.

[0024] Preferably, the bacterial liquid in step five is anaerobic bacteria, and the anaerobic bacteria are one or more composite strains of anaerobic cellulolytic bacteria, hydrolytic bacteria, hydrogen-producing acetogenic bacteria, sulfate-reducing bacteria, methylotrophic methanogens, and hydrogenotrophic methanogens.

[0025] Preferably, the inorganic salt medium in step 5 comprises, by mass volume ratio: 1 L of deionized water, NH4Cl

[0026] 1.0g, KH2PO4 0.1g, MgCI2·6H2O 0.165g, NaCI 0.8g, KCI 0.1g, CaCI2·2H2O

[0027] 0.04g.

[0028] Preferably, the vitamin solution in step 5 comprises, by mass to volume ratio, 1 L of deionized water, 5 mg of thiamine hydrochloride, 5 mg of riboflavin, 5 mg of calcium pantothenate, 5 mg of lipoic acid, 5 mg of p-aminobenzoic acid, 5 mg of niacin, 5 mg of vitamins, 5 mg of MESA mercaptoethanesulfonic acid, 2 mg of biotin, and 2 mg of folic acid.

[0029] Preferably, the solution used to adjust the pH to 7.0 in step 5 is a NaHCO3 solution or a NaOH solution.

[0030] The technical principle of the present invention is: the vitamin nutrient solution of the present application provides the substances necessary for microbial activities, as the nutrients necessary for microbial growth and metabolism, promotes the growth and metabolism of microorganisms, and can promote biochemical reactions and enzyme catalysis during anaerobic fermentation, thereby improving fermentation efficiency and yield. This method uses biomass as an exogenous additive to provide necessary nutrients such as carbohydrates, proteins, and trace elements, and optimizes the microbial metabolic pathway by adjusting the appropriate carbon-nitrogen ratio, thereby promoting the biosynthesis process of methane. In this way, the methane fermentation efficiency can be effectively improved, the microbial activity in the anaerobic digestion process can be enhanced, the degradation and conversion of organic matter can be promoted, and the methane yield can be increased.

[0031] Beneficial effects:

[0032] The present application provides a method for enhancing microbial gasification of coal using biomass nutrient additives, which has the following beneficial effects:

[0033] 1. The vitamin nutrient solution of the present invention provides substances necessary for microbial activities, which are nutrients necessary for microbial growth and metabolism, promote the growth and metabolism of microorganisms, and can promote biochemical reactions and enzyme catalysis during anaerobic fermentation, thereby improving fermentation efficiency and yield;

[0034] 2. The present invention is applicable to any environment, both aerobic and anaerobic. The carbon dioxide discharged from industrial production can be used to create an anaerobic environment, thereby realizing the utilization of carbon dioxide, reducing the greenhouse effect, and being eco-friendly.

[0035] 3. Urban organic waste should be properly disposed of in a timely manner, otherwise it will cause serious threats to the environment, such as releasing odors, breeding various pests, spreading animal pathogens, and polluting surface and groundwater. The reuse of urban waste realizes the sustainable use of energy;

[0036] 4. Various urban wastes and algae can be flexibly combined in proportions, and can achieve the expected carbon-nitrogen ratio after being mixed with coal samples from different regions. It has strong universality, low cost, and feasibility of engineering development;

[0037] 5. The mixed addition of multiple organic matters can provide necessary trace elements for microbial activities. At the same time, the combination of multiple organic matters and coal greatly enhances the stability of the entire anaerobic fermentation system, improves the utilization rate of coal and urban organic matter, and improves the gas production effect of the entire anaerobic fermentation;

[0038] 6. The present invention can ensure the continuous production of biomethane at room temperature of 20°C-36°C, without considering activating the bacteria by raising the temperature, thereby effectively reducing development costs. BRIEF DESCRIPTION OF THE DRAWINGS

[0039] Figure 1 This is a process flow chart of the method of using biomass nutrient additives to enhance microbial gasification of coal in this application. DETAILED DESCRIPTION

[0040] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0041] Example 1

[0042] A method for enhancing microbial gasification of coal using a biomass nutrient additive, wherein the biomass nutrient additive is dulse, and the coal sample is taken from Baiyinhua Coal Mine in Inner Mongolia, comprising the following steps:

[0043] Step 1: crush the coal sample from Baiyinhua Coal Mine in Inner Mongolia and the dulse algae by a crusher respectively, and pass through a 200-mesh sieve to obtain the coal powder and dulse algae powder from Baiyinhua Coal Mine in Inner Mongolia;

[0044] Step 2: taking coal powder and dulse powder from Baiyinhua Coal Mine, Inner Mongolia at a carbon-nitrogen ratio of 25;

[0045] Step 3: Mix the coal powder from Baiyinhua Coal Mine in Inner Mongolia and the dulse powder in a blender at 50-150 rpm for 3 minutes to obtain a reaction substrate;

[0046] Step 4: Treat the fermentation bottle and anaerobic fermentation box at 126°C and 2.59 MPa for 15 minutes to reduce the interference of bacteria on the experiment;

[0047] Step 5: Take 10g of the reaction substrate according to the mass volume ratio and put it into a 250ml fermentation bottle, take 50mL of inorganic salt culture medium and 50mL of bacterial solution into the fermentation bottle, and then put 1mL of vitamin solution and 1mL of trace metal solution into each fermentation bottle and adjust the pH to 7.0. After sealing, ferment and culture in a 36℃ constant temperature fermentation box. Three groups were tested in parallel. The reaction substrates of the control group were changed to 10g of pure Inner Mongolia Baiyinhua Coal Mine coal powder and 10g of pure red algae powder respectively; the purpose of the inorganic salt culture medium, bacterial solution, vitamin solution and trace metal solution is to simulate the living environment of microorganisms;

[0048] Step 6: Extract the gas produced by the reaction every 3 days at the beginning of the reaction according to the reaction time, use a gas chromatograph to detect the gas components in the fermentation system, use a pressure sensor to detect the air pressure in the bottle, and calculate the total volume of the gas in the bottle using the gas balance equation pV=nRT based on the detected air pressure in the bottle. Use a gas chromatograph to detect the component volume of methane in the gas, and then calculate the methane production efficiency using the gas production volume and time changes. The monitoring time is 30 days. The final fermentation gas production of the experimental group was 25.28ml / g, which was 20.69 times and 40.11 times the gas production of the pure coal control group and the pure biomass control group, respectively.

[0049] The coal sample in step 1 comes from the remaining coal resources in the target area for microbial gasification.

[0050] In the step 1, the biomass nutrient additive is nitrogen-containing organic matter, and the nitrogen-containing organic matter is one or more of urban sludge, animal feces, algae, and kitchen waste.

[0051] The bacterial liquid in step 5 is anaerobic bacteria, and the anaerobic bacteria are one or more composite strains of anaerobic cellulose decomposing bacteria, hydrolytic bacteria, hydrogen-producing acetogenic bacteria, sulfate-reducing bacteria, methylotrophic methanogens, and hydrogenotrophic methanogens.

[0052] The trace metal solution in step 5 includes, by mass volume ratio, 1L of deionized water, 0.8g of MnCI2·H2O, 0.4g of FeCI2·4H2O, 0.2g of CoCI2·6H2O, 0.08g of ZnCI2, 0.02g of CuCI2·2H2O, 0.02g of NiCI2·6H2O, 0.02g of Na2MoO4·2H2O, 0.02g of Na2SeO3, and 0.02g of NaWO4·2H2O. Among them, MnCI2, FeCI2, etc. provide trace elements for microorganisms.

[0053] The inorganic salt culture medium in step 5 includes, by mass volume ratio, 1L of deionized water, 1.0g of NH4CI, 0.1g of KH2PO4, 0.165g of MgCI2·6H2O, 0.8g of NaCI, 0.1g of KCI, and 0.04g of CaCI2·2H2O. Among them, NaCI, CaCI2, etc. provide trace elements for microorganisms, and NH4Cl provides nitrogen source for microorganisms.

[0054] The vitamin solution in step 5 includes, by mass volume ratio: 1L of deionized water, 5mg of thiamine hydrochloride, 5mg of riboflavin, 5mg of calcium pantothenate, 5mg of lipoic acid, 5mg of p-aminobenzoic acid, 5mg of niacin, and vitamin B 12 5mg, MESA mercaptoethanesulfonic acid 5mg, biotin 2mg, folic acid 2mg. Among them, the vitamin can be one or more of B1, B2, B3, B6, B7, B9, B12. Vitamins are some organic compounds necessary for the growth and metabolism of microorganisms. The vitamin nutrient solution provides the substances necessary for microbial activities. As the nutrients necessary for the growth and metabolism of microorganisms, it promotes the growth and metabolism of microorganisms. In the anaerobic fermentation process, it can promote biochemical reactions and enzyme catalysis, thereby improving the fermentation efficiency and yield.

[0055] The solution used to adjust the pH to 7.0 in step 5 is a NaHCO3 solution or a NaOH solution.

[0056] Example 2

[0057] A method for enhancing microbial gasification of coal using a biomass nutrient additive, wherein the biomass nutrient additive is Nannochloropsis, and the coal sample is taken from the Madiliang Coal Mine, comprising the following steps:

[0058] Step 1: The coal sample of the Madiliang coal mine and the red algae are crushed by a crusher respectively, and after crushing, the crushed coal is passed through a 200-mesh sieve to obtain the Madiliang coal powder and the Nannochloropsis algae powder;

[0059] Step 2: Take coal powder from Madiliang coal mine and Nannochloropsis algae powder at a carbon-nitrogen ratio of 25.3;

[0060] Step 3: Mix the Madiliang coal mine coal powder and the Nannochloropsis algae powder in a blender at 50-150 rpm for 3 minutes to obtain a reaction substrate;

[0061] Step 4: Treat the fermentation bottle and anaerobic fermentation box at 126°C and 2.59 MPa for 15 minutes to reduce the interference of bacteria on the experiment;

[0062] Step 5: Take 10g of the reaction substrate according to the mass volume ratio and put it into a 250ml fermentation bottle, take 50mL of inorganic salt culture medium and 50mL of bacterial solution into the fermentation bottle, and then put 1mL of vitamin solution and 1mL of trace metal solution into each fermentation bottle and adjust the pH to 7.0. After sealing, ferment and culture in a 36℃ constant temperature fermentation box. Three groups were tested in parallel. The reaction substrate of the control group was changed to 10g of pure Madiliang coal powder and 10g of pure Nannochloropsis powder respectively; the purpose of the inorganic salt culture medium, bacterial solution, vitamin solution and trace metal solution is to simulate the living environment of microorganisms;

[0063] Step 6: Extract the gas produced by the reaction every 3 days at the beginning of the reaction according to the reaction time, use a gas chromatograph to detect the gas components in the fermentation system, use a pressure sensor to detect the air pressure in the bottle, and calculate the total volume of the gas in the bottle using the gas balance equation pV=nRT based on the detected air pressure in the bottle. Use a gas chromatograph to detect the component volume of methane in the gas, and then calculate the methane production efficiency using the change in gas production volume and time. The monitoring time is 30 days. The final fermentation gas production of the experimental group was 15.57ml / g, which was 10.17 times and 21.75 times the gas production of the pure coal control group and the pure biomass control group, respectively.

[0064] The coal sample in step 1 comes from the remaining coal resources in the target area for microbial gasification.

[0065] In the step 1, the biomass nutrient additive is nitrogen-containing organic matter, and the nitrogen-containing organic matter is one or more of urban sludge, animal feces, algae, and kitchen waste.

[0066] The bacterial liquid in step 5 is anaerobic bacteria, and the anaerobic bacteria are one or more composite strains of anaerobic cellulose decomposing bacteria, hydrolytic bacteria, hydrogen-producing acetogenic bacteria, sulfate-reducing bacteria, methylotrophic methanogens, and hydrogenotrophic methanogens.

[0067] The trace metal solution in step 5 includes, by mass volume ratio, 1L of deionized water, 0.8g of MnCI2·H2O, 0.4g of FeCI2·4H2O, 0.2g of CoCI2·6H2O, 0.08g of ZnCI2, 0.02g of CuCI2·2H2O, 0.02g of NiCI2·6H2O, 0.02g of Na2MoO4·2H2O, 0.02g of Na2SeO3, and 0.02g of NaWO4·2H2O. Among them, MnCI2, FeCI2, etc. provide trace elements for microorganisms.

[0068] The inorganic salt culture medium in step 5 includes, by mass volume ratio, 1L of deionized water, 1.0g of NH4CI, 0.1g of KH2PO4, 0.165g of MgCI2·6H2O, 0.8g of NaCI, 0.1g of KCI, and 0.04g of CaCI2·2H2O. Among them, NaCI, CaCI2, etc. provide trace elements for microorganisms, and NH4Cl provides nitrogen source for microorganisms.

[0069] The vitamin solution in step 5 includes, by mass volume ratio: 1L of deionized water, 5mg of thiamine hydrochloride, 5mg of riboflavin, 5mg of calcium pantothenate, 5mg of lipoic acid, 5mg of p-aminobenzoic acid, 5mg of niacin, and vitamin B 12 5mg, MESA mercaptoethanesulfonic acid 5mg, biotin 2mg, folic acid 2mg. Among them, the vitamin can be one or more of B1, B2, B3, B6, B7, B9, B12. Vitamins are some organic compounds necessary for the growth and metabolism of microorganisms. The vitamin nutrient solution provides the substances necessary for microbial activities. As the nutrients necessary for the growth and metabolism of microorganisms, it promotes the growth and metabolism of microorganisms. In the anaerobic fermentation process, it can promote biochemical reactions and enzyme catalysis, thereby improving the fermentation efficiency and yield.

[0070] The solution used to adjust the pH to 7.0 in step 5 is a NaHCO3 solution or a NaOH solution.

[0071] Example 3

[0072] A method for enhancing microbial gasification of coal using a biomass nutrient additive, wherein the biomass nutrient additive is Nannochloropsis, and the coal sample is taken from the Madiliang Coal Mine in Inner Mongolia, comprising the following steps:

[0073] Step 1: crush the coal sample from the Madiliang Coal Mine in Inner Mongolia and the Nannochloropsis algae by a crusher, and then pass through a 200-mesh sieve to obtain the coal powder from the Baiyinhua Coal Mine in Inner Mongolia and the Nannochloropsis algae powder;

[0074] Step 2: taking coal powder from Madiliang Coal Mine in Inner Mongolia and Nannochloropsis algae powder at a carbon-nitrogen ratio of 28.96;

[0075] Step 3: Mix the coal powder from the Madiliang Coal Mine in Inner Mongolia and the Nannochloropsis algae powder in a blender at 50-150 rpm for 3 minutes to obtain a reaction substrate;

[0076] Step 4: subject the fermentation bottle and the anaerobic fermentation box to high temperature and high pressure treatment at 126°C and 2.59 MPa for 15 minutes;

[0077] Step 5: Take 10g of the reaction substrate according to the mass volume ratio and put it into a 250ml fermentation bottle, take 50mL of inorganic salt culture medium and 50mL of bacterial solution into the fermentation bottle, and then put 1mL of vitamin solution and 1mL of trace metal solution into each fermentation bottle and adjust the pH to 7.0. After sealing, ferment and culture in a 36℃ constant temperature fermentation box. Three groups were tested in parallel. The reaction substrate of the control group was changed to 10g of pure Inner Mongolia Madiliang Coal Mine coal powder and 10g of pure Nannochloropsis powder respectively; the purpose of the inorganic salt culture medium, bacterial solution, vitamin solution and trace metal solution is to simulate the living environment of microorganisms;

[0078] Step 6: Extract the gas produced by the reaction every 3 days at the beginning of the reaction according to the reaction time, use a gas chromatograph to detect the gas components in the fermentation system, use a pressure sensor to detect the air pressure in the bottle, and calculate the total volume of the gas in the bottle using the gas balance equation pV=nRT based on the detected air pressure in the bottle. Use a gas chromatograph to detect the component volume of methane in the gas, and then calculate the methane production efficiency using the gas production volume and time changes. The monitoring time is 30 days. The final fermentation gas production of the experimental group was 16.37ml / g, which was 10.69 times and 22.87 times the gas production of the pure coal control group and the pure biomass control group, respectively.

[0079] The coal sample in step 1 comes from the remaining coal resources in the target area for microbial gasification.

[0080] In the step 1, the biomass nutrient additive is nitrogen-containing organic matter, and the nitrogen-containing organic matter is one or more of urban sludge, animal feces, algae, and kitchen waste.

[0081] The bacterial liquid in step 5 is anaerobic bacteria, and the anaerobic bacteria are one or more composite strains of anaerobic cellulose decomposing bacteria, hydrolytic bacteria, hydrogen-producing acetogenic bacteria, sulfate-reducing bacteria, methylotrophic methanogens, and hydrogenotrophic methanogens.

[0082] The trace metal solution in step 5 includes, by mass volume ratio, 1L of deionized water, 0.8g of MnCI2·H2O, 0.4g of FeCI2·4H2O, 0.2g of CoCI2·6H2O, 0.08g of ZnCI2, 0.02g of CuCI2·2H2O, 0.02g of NiCI2·6H2O, 0.02g of Na2MoO4·2H2O, 0.02g of Na2SeO3, and 0.02g of NaWO4·2H2O. Among them, MnCI2, FeCI2, etc. provide trace elements for microorganisms.

[0083] The inorganic salt culture medium in step 5 includes, by mass volume ratio, 1L of deionized water, 1.0g of NH4CI, 0.1g of KH2PO4, 0.165g of MgCI2·6H2O, 0.8g of NaCI, 0.1g of KCI, and 0.04g of CaCI2·2H2O. Among them, NaCI, CaCI2, etc. provide trace elements for microorganisms, and NH4Cl provides nitrogen source for microorganisms.

[0084] The vitamin solution in step 5 includes, by mass volume ratio: 1L of deionized water, 5mg of thiamine hydrochloride, 5mg of riboflavin, 5mg of calcium pantothenate, 5mg of lipoic acid, 5mg of p-aminobenzoic acid, 5mg of niacin, and vitamin B 12 5mg, MESA mercaptoethanesulfonic acid 5mg, biotin 2mg, folic acid 2mg. Among them, the vitamin can be one or more of B1, B2, B3, B6, B7, B9, B12. Vitamins are some organic compounds necessary for the growth and metabolism of microorganisms. The vitamin nutrient solution provides the substances necessary for microbial activities. As the nutrients necessary for the growth and metabolism of microorganisms, it promotes the growth and metabolism of microorganisms. In the anaerobic fermentation process, it can promote biochemical reactions and enzyme catalysis, thereby improving the fermentation efficiency and yield.

[0085] The solution used to adjust the pH to 7.0 in step 5 is a NaHCO3 solution or a NaOH solution.

[0086] Example 4

[0087] A method for enhancing microbial gasification of coal using a biomass nutrient additive, wherein the biomass nutrient additive is spirulina, and the coal sample is taken from Shandong Xiezhuang Coal Mine, comprising the following steps:

[0088] Step 1: grind the coal sample from Xiezhuang Coal Mine of Shandong Province and Spirulina respectively by using a grinder, and then pass through a 200-mesh sieve to obtain coal powder from Xiezhuang Coal Mine of Shandong Province and Spirulina powder;

[0089] Step 2: Take Shandong Xiezhuang Coal Mine coal powder and spirulina powder according to the carbon-nitrogen ratio of 26.14;

[0090] Step 3: mixing the Shandong Xiezhuang Coal Mine coal powder and the spirulina powder in a blender at 50-150 rpm for 3 minutes to obtain a reaction substrate;

[0091] Step 4: subject the fermentation bottle and the anaerobic fermentation box to high temperature and high pressure treatment at 126°C and 2.59 MPa for 15 minutes;

[0092] Step 5: Take 10g of the reaction substrate according to the mass volume ratio and put it into a 250ml fermentation bottle, take 50mL of inorganic salt culture medium and 50mL of bacterial solution into the fermentation bottle, and then put 1mL of vitamin solution and 1mL of trace metal solution into each fermentation bottle and adjust the pH to 7.0. After sealing, ferment and culture in a 36℃ constant temperature fermentation box. Three groups were tested in parallel. The reaction substrate of the control group was changed to 10g of pure Shandong Xiezhuang Coal Mine coal powder and 10g of pure Spirulina powder respectively; the purpose of the inorganic salt culture medium, bacterial solution, vitamin solution and trace metal solution is to simulate the living environment of microorganisms;

[0093] Step 6: Extract the gas produced by the reaction every 3 days at the beginning of the reaction according to the reaction time, use a gas chromatograph to detect the components of the gas in the fermentation system, use a pressure sensor to detect the air pressure in the bottle, and calculate the total volume of the gas in the bottle using the gas balance equation pV=nRT based on the detected air pressure in the bottle. Use a gas chromatograph to detect the component volume of methane in the gas, and then calculate the methane production efficiency using the change in gas production volume and time. The monitoring time is 30 days. The final fermentation gas production of the experimental group was 17.89ml / g, which was 15.87 times and 24.38 times the gas production of the pure coal control group and the pure biomass control group, respectively.

[0094] The coal sample in step 1 comes from the remaining coal resources in the target area for microbial gasification.

[0095] In the step 1, the biomass nutrient additive is nitrogen-containing organic matter, and the nitrogen-containing organic matter is one or more of urban sludge, animal feces, algae, and kitchen waste.

[0096] The bacterial liquid in step 5 is anaerobic bacteria, and the anaerobic bacteria are one or more composite strains of anaerobic cellulose decomposing bacteria, hydrolytic bacteria, hydrogen-producing acetogenic bacteria, sulfate-reducing bacteria, methylotrophic methanogens, and hydrogenotrophic methanogens.

[0097] The trace metal solution in step 5 includes, by mass volume ratio, 1L of deionized water, 0.8g of MnCI2·H2O, 0.4g of FeCI2·4H2O, 0.2g of CoCI2·6H2O, 0.08g of ZnCI2, 0.02g of CuCI2·2H2O, 0.02g of NiCI2·6H2O, 0.02g of Na2MoO4·2H2O, 0.02g of Na2SeO3, and 0.02g of NaWO4·2H2O. Among them, MnCI2, FeCI2, etc. provide trace elements for microorganisms.

[0098] The inorganic salt culture medium in step 5 includes, by mass volume ratio, 1L of deionized water, 1.0g of NH4CI, 0.1g of KH2PO4, 0.165g of MgCI2·6H2O, 0.8g of NaCI, 0.1g of KCI, and 0.04g of CaCI2·2H2O. Among them, NaCI, CaCI2, etc. provide trace elements for microorganisms, and NH4Cl provides nitrogen source for microorganisms.

[0099] The vitamin solution in step 5 includes, by mass volume ratio: 1L of deionized water, 5mg of thiamine hydrochloride, 5mg of riboflavin, 5mg of calcium pantothenate, 5mg of lipoic acid, 5mg of p-aminobenzoic acid, 5mg of niacin, and vitamin B 12 5mg, MESA mercaptoethanesulfonic acid 5mg, biotin 2mg, folic acid 2mg. Among them, the vitamin can be one or more of B1, B2, B3, B6, B7, B9, B12. Vitamins are some organic compounds necessary for the growth and metabolism of microorganisms. The vitamin nutrient solution provides the substances necessary for microbial activities. As the nutrients necessary for the growth and metabolism of microorganisms, it promotes the growth and metabolism of microorganisms. In the anaerobic fermentation process, it can promote biochemical reactions and enzyme catalysis, thereby improving the fermentation efficiency and yield.

[0100] The solution used to adjust the pH to 7.0 in step 5 is a NaHCO3 solution or a NaOH solution.

[0101] Example 5

[0102] A method for strengthening microbial gasification of coal using biomass nutrient additives, wherein the biomass nutrient additives are a mixture of multiple algae, with a mass ratio of Spirulina: Chlorella: Dulse=1.5:0.4:1.1. The coal sample is taken from Lingxin Coal Mine in Ningxia, and the method comprises the following steps:

[0103] Step 1: The coal sample of Ningxia Lingxin Coal Mine and the algae mixture are crushed by a crusher respectively, and after crushing, the mixture is sieved through a 200-mesh sieve to obtain the Ningxia Lingxin Coal Mine coal powder and the algae mixture powder;

[0104] Step 2: taking a mixture powder of coal powder and algae from Lingxin Coal Mine, Ningxia, at a carbon-nitrogen ratio of 25.01;

[0105] Step 3: The Ningxia Lingxin Coal Mine coal powder and the algae mixture powder are stirred and mixed in a blender at 50-150 rpm for 3 minutes to obtain a reaction substrate;

[0106] Step 4: subject the fermentation bottle and the anaerobic fermentation box to high temperature and high pressure treatment at 126°C and 2.59 MPa for 15 minutes;

[0107] Step 5: 10 g of the reaction substrate was taken into a 250 ml fermentation bottle according to the mass volume ratio, 50 ml of the inorganic salt culture medium and 50 ml of the bacterial solution were taken into the fermentation bottle, and each fermentation bottle was then filled with 1 ml of the vitamin solution and 1 ml of the trace metal solution and the pH was adjusted to 7.0. After sealing, the bottles were fermented and cultured in a 36°C constant temperature fermentation box. Three groups were tested in parallel. The reaction substrates of the control group were changed to 10 g of pure Ningxia Lingxin Coal Mine coal powder and 10 g of pure algae mixture powder respectively; the inorganic salt culture medium, bacterial solution, vitamin solution and trace metal solution were used to simulate the living environment of microorganisms;

[0108] Step 6: Extract the gas produced by the reaction every 3 days at the beginning of the reaction according to the reaction time, use a gas chromatograph to detect the gas components in the fermentation system, use a pressure sensor to detect the air pressure in the bottle, and calculate the total volume of the gas in the bottle using the gas balance equation pV=nRT based on the detected air pressure in the bottle. Use a gas chromatograph to detect the component volume of methane in the gas, and then calculate the methane production efficiency using the gas production volume and time changes. The monitoring time is 30 days. The final fermentation gas production of the experimental group was 27.79ml / g, which was 24.22 times and 9.43 times the gas production of the pure coal control group and the pure biomass control group, respectively.

[0109] The coal sample in step 1 comes from the remaining coal resources in the target area for microbial gasification.

[0110] In the step 1, the biomass nutrient additive is nitrogen-containing organic matter, and the nitrogen-containing organic matter is one or more of urban sludge, animal feces, algae, and kitchen waste.

[0111] The bacterial liquid in step 5 is anaerobic bacteria, and the anaerobic bacteria are one or more composite strains of anaerobic cellulose decomposing bacteria, hydrolytic bacteria, hydrogen-producing acetogenic bacteria, sulfate-reducing bacteria, methylotrophic methanogens, and hydrogenotrophic methanogens.

[0112] The trace metal solution in step 5 includes, by mass volume ratio, 1L of deionized water, 0.8g of MnCI2·H2O, 0.4g of FeCI2·4H2O, 0.2g of CoCI2·6H2O, 0.08g of ZnCI2, 0.02g of CuCI2·2H2O, 0.02g of NiCI2·6H2O, 0.02g of Na2MoO4·2H2O, 0.02g of Na2SeO3, and 0.02g of NaWO4·2H2O. Among them, MnCI2, FeCI2, etc. provide trace elements for microorganisms.

[0113] The inorganic salt culture medium in step 5 includes, by mass volume ratio, 1L of deionized water, 1.0g of NH4CI, 0.1g of KH2PO4, 0.165g of MgCI2·6H2O, 0.8g of NaCI, 0.1g of KCI, and 0.04g of CaCI2·2H2O. Among them, NaCI, CaCI2, etc. provide trace elements for microorganisms, and NH4Cl provides nitrogen source for microorganisms.

[0114] The vitamin solution in step 5 includes, by mass volume ratio: 1L of deionized water, 5mg of thiamine hydrochloride, 5mg of riboflavin, 5mg of calcium pantothenate, 5mg of lipoic acid, 5mg of p-aminobenzoic acid, 5mg of niacin, and vitamin B 12 5mg, MESA mercaptoethanesulfonic acid 5mg, biotin 2mg, folic acid 2mg. Among them, the vitamin can be one or more of B1, B2, B3, B6, B7, B9, B12. Vitamins are some organic compounds necessary for the growth and metabolism of microorganisms. The vitamin nutrient solution provides the substances necessary for microbial activities. As the nutrients necessary for the growth and metabolism of microorganisms, it promotes the growth and metabolism of microorganisms. In the anaerobic fermentation process, it can promote biochemical reactions and enzyme catalysis, thereby improving the fermentation efficiency and yield.

[0115] The solution used to adjust the pH to 7.0 in step 5 is a NaHCO3 solution or a NaOH solution.

[0116] The present invention is not limited by the above embodiments. The above embodiments and descriptions are only for illustrating the principles of the present invention. The present invention may be subjected to various changes and modifications without departing from the spirit and scope of the present invention. These changes and modifications all fall within the scope of the present invention claimed for protection.

Claims

1. A method for enhancing microbial gasification of coal using biomass nutrient additives, characterized in that: The following steps are involved: Step 1: The coal sample and the biomass nutrient additive are crushed by a crusher respectively, and after crushing, the coal powder and the biomass powder are sieved through a 200-mesh sieve to obtain; Step 2: Take coal powder and biomass powder at a carbon-nitrogen ratio of 20-30; Step 3: Mix the coal powder and biomass powder in a mixer at 50-150 rpm for 3 minutes to obtain a reaction substrate; Step 4: subject the fermentation bottle and the anaerobic fermentation box to high temperature and high pressure treatment at 126°C and 2.59Mpa for 15 minutes; Step 5: 10 g of the reaction substrate was taken into a 250 ml fermentation bottle according to the mass volume ratio, 50 ml of inorganic salt culture medium and 50 ml of bacterial solution were taken into the fermentation bottle, and each fermentation bottle was then filled with 1 ml of vitamin solution and 1 ml of trace metal solution and the pH was adjusted to 7.

0. After sealing, the fermentation was carried out in a 36 ° C constant temperature fermentation box. Three groups were tested in parallel. The reaction substrates of the control group were changed to 10 g of pure coal powder and 10 g of pure biomass powder respectively; Step six: According to the reaction time, extract the gas produced by the reaction every 3 days after the reaction starts, use a gas chromatograph to detect the components of the gas in the fermentation system, use a pressure sensor to detect the air pressure in the bottle, and use the gas balance equation pV=nRT to calculate the total volume of the gas in the bottle based on the detected air pressure in the bottle. Use a gas chromatograph to detect the component volume of methane in the gas, and then use the change in gas production volume and time to calculate the methane production efficiency. The monitoring period is 30 days.

2. The method for enhancing microbial gasification of coal using biomass nutrient additives according to claim 1, characterized in that: The coal sample in step 1 comes from the remaining coal resources in the target area for microbial gasification.

3. The method for enhancing microbial gasification of coal using biomass nutrient additives according to claim 2, characterized in that: The coal samples are sourced from the Baiyinhua Coal Mine in Inner Mongolia, the Madiliang Coal Mine in Inner Mongolia, the Xiezhuang Coal Mine in Shandong or the Lingxin Coal Mine in Ningxia.

4. The method for enhancing microbial gasification of coal using biomass nutrient additives according to claim 1, characterized in that: In the step 1, the biomass nutrient additive is nitrogen-containing organic matter.

5. The method for enhancing microbial gasification of coal using biomass nutrient additives according to claim 4, characterized in that: The nitrogen-containing organic matter is one or more of urban sludge, animal feces, algae, and kitchen waste.

6. The method for enhancing microbial gasification of coal using biomass nutrient additives according to claim 5, characterized in that: The algae are one or more of dulse, Nannochloropsis, Spirulina, and Chlorella pyrenoidosa.

7. The method for enhancing microbial gasification of coal using biomass nutrient additives according to claim 1, characterized in that: The bacterial liquid in step 5 is anaerobic bacteria, and the anaerobic bacteria are one or more composite strains of anaerobic cellulose decomposing bacteria, hydrolytic bacteria, hydrogen-producing acetogenic bacteria, sulfate-reducing bacteria, methylotrophic methanogens, and hydrogenotrophic methanogens.

8. The method for enhancing microbial gasification of coal using biomass nutrient additives according to claim 1, characterized in that: The inorganic salt culture medium in step 5 includes, by mass volume ratio, 1 L of deionized water, 1.0 g of NH4CI, 0.1 g of KH2PO4, 0.165 g of MgCI2·6H2O, 0.8 g of NaCI, 0.1 g of KCI, and 0.04 g of CaCI2·2H2O.

9. The method for enhancing microbial coal gasification by using biomass nutrient additives according to claim 1, characterized in that: The vitamin solution in step 5 includes, by mass to volume ratio, 1 L of deionized water, 5 mg of thiamine hydrochloride, 5 mg of riboflavin, 5 mg of calcium pantothenate, 5 mg of lipoic acid, 5 mg of p-aminobenzoic acid, 5 mg of niacin, 5 mg of vitamins, 5 mg of MESA mercaptoethanesulfonic acid, 2 mg of biotin, and 2 mg of folic acid.

10. The method of enhancing microbial gasification of coal using biomass nutrient additives according to claim 1, characterized in that: The solution used to adjust the pH to 7.0 in step 5 is a NaHCO3 solution or a NaOH solution.

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