Automatic sample preliminary screening detection equipment based on magnetic solid-phase extraction

Abstract

The invention discloses automatic sample preliminary screening detection equipment based on magnetic solid-phase extraction. The detection equipment is integrally arranged, automatic operation is achieved from a sample to a detection result to maximize, and the equipment is simple in structure, stable in operation and high in detection structure accuracy. The magnetic bar assembly, the magnetic sleeve assembly, the sample groove assembly, the centrifugal tube assembly and the detection device are respectively in power connection with the independent power device, and each assembly independently runs through the power device, so that the running stability of the equipment is improved; the magnetic bar assembly, the magnetic sleeve assembly, the sample groove assembly and the centrifugal tube assembly achieve sample screening through simple vertical reciprocating motion and horizontal reciprocating motion respectively, the structure is simple, operation is convenient, running is stable, and the sample screening accuracy is high; the sample groove moves back and forth to enrich a target object, sample mixing and target object enrichment can be realized without left-right movement of the magnetic sleeve, an action power device is omitted, the equipment operation stability is improved, and the detection accuracy is improved.

Description

Technical Field

[0001] The invention relates to the technical field of magnetic solid phase extraction detection equipment, and in particular to an automated sample primary screening detection equipment based on magnetic solid phase extraction. Background Art

[0002] Magnetic solid phase extraction is a new type of solid phase extraction technology developed in recent years. It is a method for separation, enrichment and detection based on the specific interaction between magnetic particles and target substances. The basic principle of magnetic solid phase extraction technology is to use magnetic or magnetically modified adsorbents to adsorb the analyzed substances without adsorbing impurities such as proteins. At the same time, magnetically modified adsorbents can aggregate and disperse under the action of an external magnetic field to complete the mixing, pipetting and washing steps, thereby achieving the purpose of separating the target substance from the impurities. Afterwards, the target substance is released from the eluent and the extraction is finally completed.

[0003] Magnetic solid phase extraction technology is widely used in biomedicine, environmental monitoring, food safety and other fields, such as DNA / RNA extraction, protein purification, drug screening, water quality monitoring, pesticide residue research, etc. Its advantages are simple operation, rapid and efficient, high sensitivity, high purity and high throughput.

[0004] In the related art, the initial screening detection equipment based on solid phase extraction has the following disadvantages:

[0005] 1. The initial screening detection equipment currently on the market that uses the SPE solid phase extraction method can only test 1-2 samples at the same time, and the number of samples tested at a single time is relatively low; a variety of organic reagents are used during the operation, the steps are cumbersome, and high operating requirements are placed on the operator.

[0006] 2. The volume of nucleic acid extraction instruments commonly used in the market using magnetic beads is about 1 ml, and the sample volume that can be enriched is relatively small. The enrichment effect is poor for large volumes, such as 50 mL, that is, it is difficult to extract trace substances in large volume samples.

[0007] 3. The sample screening and testing instruments on the market are all devices that separate the concentration extraction and detection phases, with a low degree of integration. It is very inconvenient for grassroots personnel to carry two sets of devices on site, and the portability is low.

[0008] Therefore, in practical applications, an automated sample screening and detection equipment based on magnetic solid phase extraction is needed, which has an integrated design, simple structure, simple operation, high sample detection efficiency, and high degree of automation. Summary of the invention

[0009] In response to the problems in the related art, the present application discloses an automated sample screening and detection device based on magnetic solid phase extraction, which solves the related problems in the background technology.

[0010] To achieve the above objectives, this application provides the following technical solutions:

[0011] An automated sample screening and detection device based on magnetic solid phase extraction, comprising a housing,

[0012] The housing is provided with a control unit, a power unit, a screening unit, a detection unit, a printing unit and a display unit;

[0013] The control unit includes a control panel, which is fixedly mounted on a control bracket and connected to the signal control of each unit;

[0014] The power unit includes multiple groups of power devices which are respectively connected to various parts of the detection equipment to realize the movement operation of each component;

[0015] The screening part includes a sample slot assembly, a magnetic rod assembly, a magnetic sleeve assembly and a centrifuge tube assembly.

[0016] The sample tank assembly pre-stores the sample to be tested and mixes it.

[0017] The magnetic rod assembly cooperates with the magnetic sleeve assembly to enrich the target in the sample slot assembly, and the magnetic rod assembly cooperates with the magnetic sleeve assembly to extract the target into the centrifuge tube assembly, and absorbs the target solution and drips it into the reagent card;

[0018] The detection unit includes a detection device, a card holder device is provided at the lower part of the detection device, the card holder device is connected to the detection power device, the detection power device can drive the card holder device to move in and out, the card holder device can hold a reagent card and carry the reagent card in and out, and the detection device scans the information on the reagent card and performs detection readings;

[0019] The printing unit includes a printer, and the printer prints and outputs the detection data of the detection unit;

[0020] The display unit includes a display screen, which is arranged outside the shell and is used to operate the device and display data.

[0021] As a further solution of the present application: the sample slot assembly includes a plurality of sample slots for pre-storing samples, the sample slots are in the shape of long strips, and the sample slots are arranged horizontally.

[0022] The magnetic bar assembly comprises a magnetic bar, and the magnetic bar is connected to a magnetic bar power device, and the magnetic bar power device drives the magnetic bar to move.

[0023] The magnetic sleeve assembly includes a magnetic sleeve, and the magnetic sleeve is connected to a magnetic sleeve power device, and the magnetic sleeve power device drives the magnetic sleeve to move.

[0024] The centrifuge tube assembly comprises a centrifuge tube and a heating device, wherein the heating device surrounds the bottom and sides of the centrifuge tube and performs heating operation on the centrifuge tube.

[0025] The sample slot assembly and the centrifuge tube assembly are arranged in parallel on the upper part of the moving plate, and a plurality of moving wheels are arranged on the lower part of the moving plate. The moving plate is connected to a moving power device, and the moving power device drives the moving plate to move forward and backward.

[0026] The detection device includes a detection shell, in which a detection camera and an annular light source are arranged. The center of the annular light source is hollow. The detection camera is arranged at the top position of the center of the annular light source. The detection camera scans information on the reagent card at the bottom and performs detection readings.

[0027] As a further solution of the present application: the magnetic rod power device and the magnetic sleeve power device are vertical power devices, the magnetic rod assembly is arranged above the magnetic sleeve assembly, and the magnetic rod power device and the magnetic sleeve power device respectively drive the magnetic rod and the magnetic sleeve to move or vibrate independently or relatively up and down,

[0028] The mobile power device is a horizontal power device, the mobile plate is arranged below the magnetic sleeve assembly, and the mobile power device drives the sample slot assembly and the centrifuge tube assembly to move forward and backward relative to the magnetic sleeve assembly through the mobile plate.

[0029] The sample tank moves to the bottom of the magnetic sleeve, and the magnetic sleeve moves down to the inside of the sample tank. The sample tank reciprocates back and forth to achieve sample mixing.

[0030] The magnetic rod moves down to the bottom of the magnetic sleeve, and the magnetic rod and the magnetic sleeve are located together inside the sample slot. The sample slot moves back and forth to achieve target enrichment.

[0031] The magnetic rod and the magnetic sleeve are moved out of the sample tank as a whole, the centrifuge tube is moved to the bottom of the magnetic sleeve, the magnetic rod is stationary, the magnetic sleeve moves downward to the bottom of the centrifuge tube, and the magnetic sleeve vibrates to separate the target.

[0032] The centrifuge tube is pre-filled with an elution solution, and the heating device heats the centrifuge tube to complete the elution of the target object.

[0033] As a further solution of the present application: the magnetic sleeve moves down to the inside of the sample slot, and the sample slot reciprocates back and forth 10-20 times to achieve sample mixing operation.

[0034] As a further solution of the present application: the magnetic rod moves down to the bottom of the magnetic sleeve, the magnetic rod and the magnetic sleeve are located together inside the sample slot, the sample slot is allowed to stand at multiple points, and after the sample slot is allowed to stand, it moves to the next point to continue standing, and the sample slot achieves target enrichment after being allowed to stand at multiple points in turn.

[0035] As a further solution of the present application: the static time at each point is 80-120 seconds.

[0036] As a further solution of the present application: the magnetic sleeve vibrates in the centrifuge tube for 20-40 seconds to separate the target object.

[0037] As a further solution of the present application: the heating device heats the centrifuge tube to a temperature of 60-80 degrees Celsius.

[0038] In summary, the beneficial effects of this application are:

[0039] 1. The automated sample screening and detection equipment based on magnetic solid phase extraction has a control unit, a power unit, a screening unit, a detection unit, a printing unit and a display unit inside the shell; the control unit performs signal control connection on various units in the equipment to control the coordinated operation of various units; the power unit includes multiple groups of power devices which are respectively connected to various power units of the detection equipment to realize the movement operation of various components; the screening unit enriches and extracts the target in the sample, and the detection device scans the reagent card information and performs detection readings; the printing unit prints out the detection data of the detection unit; the display unit operates the equipment and displays the data; this new detection equipment integrates the control unit, power unit, screening unit, detection unit, printing unit and display unit, and maximizes the automated operation from sample to detection result. The equipment has a simple structure, stable operation and high detection structure accuracy.

[0040] 2. The magnetic rod assembly and the magnetic sleeve assembly are separately connected to the independent power device. The sample slot is horizontally arranged, and the sample slot assembly and the centrifuge tube assembly are arranged side by side on the upper part of the moving plate. The sample slot assembly and the centrifuge tube assembly are driven to move forward and backward by the moving power device through the moving plate and the moving wheel. The magnetic rod assembly, the magnetic sleeve assembly, the sample slot assembly and the centrifuge tube assembly are respectively connected to the independent power device. Each assembly operates independently through the power device, which improves the operation stability of the equipment. The heating device is arranged to surround the bottom and sides of the centrifuge tube, which has a higher heating efficiency.

[0041] 3. The magnetic rod power device and the magnetic sleeve power device are vertical power devices. The magnetic rod assembly is arranged above the magnetic sleeve assembly. The magnetic rod power device and the magnetic sleeve power device respectively drive the magnetic rod and the magnetic sleeve to move up and down or vibrate independently or relatively. The mobile power device is a horizontal power device. The moving plate is arranged below the magnetic sleeve assembly. The mobile power device drives the sample slot assembly and the centrifuge tube assembly to move back and forth relative to the magnetic sleeve assembly through the moving plate. The magnetic rod assembly, the magnetic sleeve assembly, the sample slot assembly and the centrifuge tube assembly respectively realize sample screening through simple vertical reciprocating motion and horizontal reciprocating motion. The structure is simple, the operation is convenient, the operation is stable, and the sample screening accuracy is high.

[0042] 4. The magnetic sleeve moves down to the inside of the sample slot, and the sample slot moves back and forth to achieve sample mixing. The magnetic rod and the magnetic sleeve are located together in the sample slot, and the sample slot moves back and forth to achieve target enrichment. Sample mixing and target enrichment can be achieved without the magnetic sleeve moving left and right, omitting a motion power device, improving the equipment operation stability and improving the detection accuracy.

[0043] 5. The magnetic rod and the magnetic sleeve are located together inside the sample tank. The sample tank is allowed to stand at multiple points. After the sample tank stands still, it moves to the next point to continue standing still. The sample tank is allowed to stand still at multiple points in sequence to achieve target enrichment. The target is enriched by standing still at multiple points in sequence, completing the all-round separation of the sample solution in the sample tank and improving the extraction efficiency and accuracy of the sample target. BRIEF DESCRIPTION OF THE DRAWINGS

[0044] The accompanying drawings are used to provide further understanding of the present application and constitute a part of the specification. They are used to explain the present application together with the embodiments of the present application and do not constitute a limitation of the present application.

[0045] In the attached picture:

[0046] Figure 1 This is a schematic diagram of the shell structure of this application.

[0047] Figure 2 This is a schematic diagram of the structure inside the shell of this application.

[0048] Figure 3 This is a schematic diagram of the structure of the screening part of this application.

[0049] Figure 4 This is a schematic diagram of the top view of the screening part of this application.

[0050] Figure 5 This is a schematic diagram of the top view of the detection device of the present application.

[0051] Figure 6 This is a schematic diagram of the structure of the detection device of the present application from a bottom up view.

[0052] Reference numerals:

[0053] 01. Shell; 02. Screening cover; 03. Inlet and outlet; 11. Control bracket; 12. Control board; 21. Detection power motor; 22. Magnetic rod power motor; 23. Magnetic sleeve power motor; 24. Mobile power motor; 31. Magnetic rod; 32. Magnetic sleeve; 33. Sample slot; 34. Centrifuge tube; 35. Heating device; 36. Mobile plate; 37. Mobile wheel; 38. Support frame; 41. Detection device; 411. Detection shell; 412. Detection camera; 413. Ring light source; 42. Card tray device; 51. Printer; 61. Display screen; DETAILED DESCRIPTION

[0054] Exemplary embodiments will be described in detail herein, examples of which are shown in the accompanying drawings. When the following description refers to the drawings, the same numbers in different drawings represent the same or similar elements unless otherwise indicated. The implementations described in the following exemplary embodiments do not represent all implementations consistent with the present application. Instead, they are merely examples of devices and methods consistent with some aspects disclosed in the present embodiments as detailed in the appended claims.

[0055] It should be noted that all directional indications in the embodiments (such as up, down, left, right, front, back, etc.) are only used to explain the relative position relationship, movement status, etc. between the components under a certain specific posture (as shown in the accompanying drawings). If the specific posture changes, the directional indication will also change accordingly.

[0056] In addition, the descriptions of "first", "second", etc. in the embodiments are only for descriptive purposes, and do not specifically refer to the order or sequence, nor are they used to limit the present application. They are only used to distinguish components or operations described with the same technical terms, and cannot be understood as indicating or implying their relative importance or implicitly indicating the number of technical features indicated. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of the features. In addition, the technical solutions between the various embodiments can be combined with each other, but they must be based on the ability of ordinary technicians in the field to implement them. When the combination of technical solutions is contradictory or cannot be implemented, it should be considered that such a combination of technical solutions does not exist and is not within the scope of protection required by the present application.

[0057] In order to further understand the content, features and effects of the present application, the following embodiments are given as examples and described in detail with reference to the accompanying drawings:

[0058] like Figure 1-4 As shown:

[0059] The automated sample screening and detection equipment based on magnetic solid phase extraction comprises a housing 01, wherein a control unit, a power unit, a screening unit, a detection unit, a printing unit and a display unit are arranged inside the housing 01.

[0060] The control unit includes a control panel 12 , which is fixedly mounted on the control bracket 11 , and is connected to the signal control of each unit.

[0061] The power unit includes multiple power devices, namely, a detection power motor 21, a magnetic rod power motor 22, a magnetic sleeve power motor 23, and a moving power motor 24, which are respectively connected to the power of each part of the detection equipment to realize the movement operation of each component.

[0062] The screening part includes a sample slot assembly, a magnetic rod assembly, a magnetic sleeve assembly and a centrifuge tube assembly. The sample to be tested is pre-stored and mixed inside the sample slot assembly. The magnetic rod assembly cooperates with the movement of the magnetic sleeve assembly to enrich the target in the sample slot assembly. The magnetic rod assembly cooperates with the movement of the magnetic sleeve assembly to extract the target into the centrifuge tube assembly, and the target solution is absorbed and dripped into the reagent card.

[0063] The detection part includes a detection device 41, which includes a detection shell 411. A detection camera 412 and a ring-shaped light source 413 are arranged inside the detection shell 411. The ring-shaped light source 413 is an LED ring-shaped fill light. The center of the ring-shaped light source 413 is hollow. The detection camera 412 is arranged at the top position of the center of the ring-shaped light source 413. The detection camera 412 scans the information of the reagent card at the bottom and performs detection readings.

[0064] A card holder device 42 is provided at the lower part of the detection device 41, and the card holder device 42 is connected to the detection power device. The detection power device can drive the card holder device 42 to move in and out. The card holder device 42 can hold a reagent card and carry the reagent card in and out. The detection device 41 scans the information on the reagent card and performs detection readings.

[0065] The printing unit includes a printer 51, and the printer 51 prints out the detection data of the detection unit.

[0066] The display unit includes a display screen 61, which is arranged outside the housing 01. The display screen 61 operates the equipment and displays data.

[0067] This new testing equipment integrates the control unit, power unit, screening unit, testing unit, printing unit and display unit, and maximizes the automation operation from sample to test result. The equipment has a simple structure, stable operation and high detection structure accuracy.

[0068] Specifically, the housing 01 is provided with a display screen 61 on the operation surface, and the housing 01 is provided with a printer 51 on the other side surface.

[0069] The shell 01 is provided with a reagent card inlet and outlet 03 on the operating surface, and a card holder device 42 is connected inside the inlet and outlet 03. A detection device 41 is provided on the upper part of the card holder device 42. The card holder device 42 is connected to the detection power motor 21 through a synchronous belt. The detection power motor 21 drives the card holder device 42 to carry the reagent card in and out through the synchronous belt. The reagent card enters the lower part of the detection device 41 to realize information scanning and detection reading. The reagent card is ejected from the inlet and outlet 03 to realize the taking and placing of the reagent card.

[0070] The housing 01 is provided with a screening cover plate 02 on the operation surface, the screening cover plate 02 is connected to the housing 01, and a screening part is provided inside the screening cover plate 02. The screening cover plate 02 is opened to install and replace the sample slot 33, the magnetic sleeve 32 and the centrifuge tube 34. The sample slot 33 is pre-loaded with a sample, and the centrifuge tube 34 is pre-loaded with an eluent.

[0071] A support frame 38 is provided inside the shell 01, and a magnetic rod power motor 22 and a magnetic sleeve power motor 23 are provided side by side on the top of the support frame 38. The magnetic rod power motor 22 and the magnetic sleeve power motor 23 are vertical power motors. The magnetic rod power motor 22 and the magnetic sleeve power motor 23 are respectively connected to the magnetic rod 31 and the magnetic sleeve 32 through a ball screw assembly. The magnetic rod 31 and the magnetic sleeve 32 are slidingly connected to the support frame 38 through a slide rail structure. Under the action of the motor, the magnetic rod 31 and the magnetic sleeve 32 transmit power through the ball screw assembly, and the magnetic rod 31 and the magnetic sleeve 32 move up and down along the support frame 38 through the slide rail structure.

[0072] The magnetic rod 31 is arranged above the magnetic sleeve 32 and can be inserted into the magnetic sleeve 32. The magnetic rod power motor 22 and the magnetic sleeve power motor 23 respectively drive the magnetic rod 31 and the magnetic sleeve 32 to move or vibrate up and down independently or relatively.

[0073] A control bracket 11 is fixedly provided at the rear of the support frame 38 , and a control panel 12 is fixedly provided on the control bracket 11 .

[0074] A sample slot assembly and a centrifuge tube assembly are provided below the support frame 38 .

[0075] The sample slot assembly includes four sample slots 33 for pre-storing samples. The sample slots 33 are in the shape of long strips and are arranged horizontally.

[0076] The centrifuge tube assembly includes four centrifuge tubes 34 and a heating device 35 . The heating device 35 is disposed around the bottom and sides of the centrifuge tubes 34 , and the heating device 35 performs a heating operation on the centrifuge tubes 34 .

[0077] Four sample slots 33 and four centrifuge tubes 34 are arranged in parallel on the upper part of the movable plate 36, and four movable wheels 37 are provided at the lower part of the movable plate 36. The movable plate 36 is connected to the movable power motor 24 through a synchronous belt power. The movable power motor 24 drives the movable plate 36 to move forward and backward through the synchronous belt. The forward and backward movement of the movable plate 36 realizes the forward and backward movement of the sample slots 33 and the centrifuge tubes 34.

[0078] The automated operation of the sample in the screening section includes the following steps:

[0079] The sample slot 33 moves to the bottom of the magnetic sleeve 32, and the magnetic sleeve 32 moves down to the inside of the sample slot 33. The sample slot 33 reciprocates 10-20 times to achieve sample mixing operation.

[0080] Preferably, the sample slot 33 reciprocates 15 times, and the reciprocating motion takes a total of 120 seconds to achieve sample mixing.

[0081] Preferably, the sample slot 33 reciprocates 10 times, and the reciprocating motion takes a total of 140 seconds to achieve sample mixing.

[0082] The magnetic rod 31 moves down to the bottom of the magnetic sleeve 32. The magnetic rod 31 and the magnetic sleeve 32 are located together inside the sample slot 33. The sample slot 33 is allowed to stand at multiple points. The sample slot 33 stands at each point for 80-120 seconds. After standing, it moves to the next point to continue standing. The sample slot 33 is allowed to stand at multiple points in turn to achieve target enrichment.

[0083] Preferably, the sample tank 33 is left standing at six positions in sequence, and the standing time at each position is 90 seconds.

[0084] Preferably, the sample tank 33 is left standing at eight positions in sequence, and the standing time at each position is 110 seconds.

[0085] The magnetic rod 31 and the magnetic sleeve 32 are slowly moved out of the sample slot 33 as a whole, the centrifuge tube 34 moves to the bottom of the magnetic sleeve 32, the magnetic rod 31 is stationary, the magnetic sleeve 32 moves downward to the inside of the centrifuge tube 34, and the magnetic sleeve 32 vibrates in the centrifuge tube 34 for 20-40 seconds to separate the target. Preferably, the magnetic sleeve 32 vibrates in the centrifuge tube 34 for 30 seconds to separate the target.

[0086] The heating device 35 heats the centrifuge tube 34 to complete the elution of the target object. The outside of the heating device 35 is provided with an insulation layer to improve the heating efficiency of the centrifuge tube 34. The inside of the heating device 35 also includes a temperature sensor to achieve precise temperature control. The heating temperature of the centrifuge tube 34 by the heating device 35 is 60-80 degrees Celsius.

[0087] The preferred heating temperature is 70 degrees Celsius.

[0088] Open the screening cover 02, remove the centrifuge tube 34, draw the target solution and drip it into the reagent card, insert the reagent card into the card holder device 42, the detection device 41 scans the information and detects the readings of the reagent card, and the printer 51 prints out the detection data of the detection unit.

[0089] In practical application:

[0090] The new testing equipment integrates the control unit, power unit, screening unit, testing unit, printing unit and display unit, maximizing the automation of operations from sample to test result. The equipment has a simple structure, stable operation and high detection structure accuracy.

[0091] The magnetic bar assembly and the magnetic sleeve assembly are separately connected to the power of an independent power device. The sample slot 33 is horizontally arranged, and the sample slot assembly and the centrifuge tube assembly are arranged in parallel on the upper part of the movable plate 36. The sample slot assembly and the centrifuge tube assembly are driven to move back and forth by the movable power device through the movable plate 36 and the movable wheel 37. The magnetic bar assembly, the magnetic sleeve assembly, the sample slot assembly and the centrifuge tube assembly are respectively connected to the power of an independent power device. Each assembly operates independently through the power device, thereby improving the operating stability of the equipment.

[0092] This new detection equipment can efficiently enrich samples of larger volumes, and has a better enrichment effect on large volumes, such as 50mL samples. In other words, trace substances are easier to extract in large volume samples, and the extraction efficiency is higher.

[0093] This new detection equipment realizes integrated design and automated operation, and realizes automated operation of extraction, detection and data display, which greatly shortens the sample concentration and extraction time and improves detection efficiency and accuracy.

[0094] The heating device 35 is disposed around the bottom and sides of the centrifuge tube 34 to increase the heating efficiency.

[0095] The magnetic rod power device and the magnetic sleeve power device are vertical power devices. The magnetic rod assembly is arranged above the magnetic sleeve assembly. The magnetic rod power device and the magnetic sleeve power device respectively drive the magnetic rod 31 and the magnetic sleeve 32 to move up and down or vibrate independently or relatively. The mobile power device is a horizontal power device. The moving plate 36 is arranged below the magnetic sleeve assembly. The mobile power device drives the sample slot assembly and the centrifuge tube assembly to move back and forth relative to the magnetic sleeve assembly through the moving plate 36. The magnetic rod assembly, the magnetic sleeve assembly, the sample slot assembly and the centrifuge tube assembly respectively realize sample screening through simple vertical reciprocating motion and horizontal reciprocating motion. The structure is simple, the operation is convenient, the operation is stable, and the sample screening accuracy is high.

[0096] The magnetic sleeve 32 moves down to the inside of the sample slot 33, and the sample slot 33 reciprocates back and forth to achieve sample mixing. The magnetic rod 31 and the magnetic sleeve 32 are jointly located inside the sample slot 33, and the sample slot 33 moves back and forth to achieve target enrichment. Sample mixing and target enrichment can be achieved without the magnetic sleeve 32 moving left and right, thereby omitting an action power device, thereby improving the operating stability of the equipment and the detection accuracy.

[0097] The magnetic rod 31 and the magnetic sleeve 32 are located together inside the sample slot 33. The sample slot 33 is allowed to stand at multiple points. After the sample slot 33 stands still, it moves to the next point to continue standing still. After the sample slot 33 stands still at multiple points in sequence, the target object is enriched. The multiple points are allowed to stand still in sequence to enrich the target object, which completes the all-round separation of the sample solution in the sample slot 33, thereby improving the extraction efficiency and accuracy of the sample target object.

[0098] Finally, it should be noted that the above disclosure is only a preferred embodiment of the present application and is not intended to limit the present application. Although the present application is described in detail with reference to the aforementioned embodiments, it is still possible for a person skilled in the art to modify the technical solutions described in the aforementioned embodiments or to replace some of the technical features therein by equivalents. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present application shall be included in the scope of protection of the present application. The scope of the present application is limited only by the attached claims.

Claims

1. An automated sample screening and detection device based on magnetic solid phase extraction, comprising a housing (01), characterized in that: The housing (01) is provided with a control unit, a power unit, a screening unit, a detection unit, a printing unit and a display unit; The control unit comprises a control panel (12), the control panel (12) is fixedly mounted on a control bracket (11), and the control panel (12) is connected to the signal control of each unit; The power unit includes multiple groups of power devices which are respectively connected to various parts of the detection equipment to realize the movement operation of each component; The screening part includes a sample slot assembly, a magnetic rod assembly, a magnetic sleeve assembly and a centrifuge tube assembly. The sample tank assembly pre-stores the sample to be tested and mixes it. The magnetic rod assembly cooperates with the magnetic sleeve assembly to enrich the target in the sample slot assembly, and the magnetic rod assembly cooperates with the magnetic sleeve assembly to extract the target into the centrifuge tube assembly, and absorbs the target solution and drips it into the reagent card; The detection unit comprises a detection device (41), a card holder device (42) is provided at the lower part of the detection device (41), the card holder device (42) is connected to the detection power device, the detection power device can drive the card holder device (42) to move in and out, the card holder device (42) can hold a reagent card and carry the reagent card in and out, and the detection device (41) scans information on the reagent card and performs detection readings; The printing unit comprises a printer (51), and the printer (51) prints out the detection data of the detection unit; The display unit comprises a display screen (61), and the display screen (61) is arranged outside the housing (01). The display screen (61) operates the equipment and displays data.

2. The automated sample screening and detection device based on magnetic solid phase extraction according to claim 1, characterized in that: The sample slot assembly comprises a plurality of sample slots (33) for pre-storing samples, wherein the sample slots (33) are in the shape of long strips of slots and are arranged horizontally. The magnetic bar assembly comprises a magnetic bar (31), wherein the magnetic bar (31) is dynamically connected to a magnetic bar power device, and the magnetic bar power device drives the magnetic bar (31) to move. The magnetic sleeve assembly comprises a magnetic sleeve (32), wherein the magnetic sleeve (32) is connected to a magnetic sleeve power device, and the magnetic sleeve power device drives the magnetic sleeve (32) to move. The centrifuge tube assembly comprises a centrifuge tube (34) and a heating device (35), wherein the heating device (35) is arranged to surround the bottom and sides of the centrifuge tube (34), and the heating device (35) performs a heating operation on the centrifuge tube (34). The sample slot assembly and the centrifuge tube assembly are arranged in parallel on the upper part of the moving plate (36), and a plurality of moving wheels (37) are arranged on the lower part of the moving plate (36). The moving plate (36) is connected to a moving power device, and the moving power device drives the moving plate (36) to move forward and backward. The detection device (41) comprises a detection shell (411), wherein a detection camera (412) and an annular light source (413) are arranged inside the detection shell (411), wherein the center of the annular light source (413) is hollow, and the detection camera (412) is arranged at the top center of the annular light source (413), and the detection camera (412) scans information on a reagent card at the bottom and performs detection readings.

3. The automated sample screening and detection device based on magnetic solid phase extraction according to claim 2, characterized in that: The magnetic rod power device and the magnetic sleeve power device are vertical power devices. The magnetic rod assembly is arranged above the magnetic sleeve assembly. The magnetic rod power device and the magnetic sleeve power device respectively drive the magnetic rod (31) and the magnetic sleeve (32) to move or vibrate up and down independently or relatively. The moving power device is a horizontal power device, the moving plate (36) is arranged below the magnetic sleeve assembly, and the moving power device drives the sample slot assembly and the centrifuge tube assembly to move forward and backward relative to the magnetic sleeve assembly through the moving plate (36). The sample slot (33) moves below the magnetic sleeve (32), the magnetic sleeve (32) moves down to the inside of the sample slot (33), and the sample slot (33) reciprocates back and forth to achieve a sample mixing operation. The magnetic rod (31) moves down to the bottom of the magnetic sleeve (32), the magnetic rod (31) and the magnetic sleeve (32) are located together inside the sample slot (33), and the sample slot (33) moves back and forth to achieve target enrichment. The magnetic rod (31) and the magnetic sleeve (32) are moved out of the sample tank (33) as a whole, the centrifuge tube (34) is moved to the bottom of the magnetic sleeve (32), the magnetic rod (31) is stationary, the magnetic sleeve (32) moves downward to the inside of the centrifuge tube (34), and the magnetic sleeve (32) vibrates to separate the target object. The centrifuge tube (34) is pre-filled with an eluent, and the heating device (35) heats the centrifuge tube (34) to complete the elution of the target object.

4. The automated sample screening and detection device based on magnetic solid phase extraction according to claim 3, characterized in that: The magnetic sleeve (32) moves down to the interior of the sample slot (33), and the sample slot (33) reciprocates back and forth 10-20 times to achieve a sample mixing operation.

5. The automated sample screening and detection device based on magnetic solid phase extraction according to claim 3, characterized in that: The magnetic rod (31) moves down to the bottom of the magnetic sleeve (32), and the magnetic rod (31) and the magnetic sleeve (32) are located together in the sample slot (33). The sample slot (33) is stationary at multiple points. After the sample slot (33) is stationary, it moves to the next point to continue to be stationary. After the sample slot (33) is stationary at multiple points in sequence, target enrichment is achieved.

6. The automated sample screening and detection device based on magnetic solid phase extraction according to claim 5, characterized in that: The static time at each point is 80-120 seconds.

7. The automated sample screening and detection device based on magnetic solid phase extraction according to claim 3, characterized in that: The magnetic sleeve (32) vibrates in the centrifuge tube (34) for 20-40 seconds to separate the target objects.

8. The automated sample screening and detection device based on magnetic solid phase extraction according to claim 3, characterized in that: The heating device (35) heats the centrifuge tube (34) to a temperature of 60-80 degrees Celsius.

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