Circular polarization luminescence detection device and detection method

Abstract

The invention discloses a circular polarization luminescence detection device which comprises an excitation light source, a sample tank arranged at the output end of the excitation light source, a first lens arranged between the sample tank and the excitation light source, and a photoelasticity regulator arranged at the output end of the sample tank and comprising a second lens and a linear polarizer, a circular polarizer capable of rotating leftwards or rightwards is arranged between the second lens and the linear polarizer, the photon detector is arranged at the output end of the photoelastic regulator, the left-rotating circularly polarized light or the right-rotating circularly polarized light can be switched to pass through the linear polarizer by adjusting the fast axis and the slow axis of the quarter-wave plate, the light is further converted into linear polarized light, and the linear polarized light is transmitted to the photon detector. Therefore, the circularly polarized light intensity can be obtained by comparing the left-handed or right-handed circularly polarized light intensity difference, the operation is simple, the signal is accurate, and the invention belongs to the technical field of optical detection.

Description

Technical Field

[0001] The present invention belongs to the technical field of optical detection, and more specifically relates to a circularly polarized luminescence detection device and a detection method. Background Art

[0002] Circularly polarized luminescence, as a manifestation of excited state chirality, has one more chirality dimension than ordinary luminescence, and has broad application prospects in the fields of three-dimensional imaging, high contrast display, and encrypted communication. Usually, optical chirality is represented by circular dichroism CD and circularly polarized luminescence CPL to represent the ground state and excited state chirality respectively.

[0003] As an excited state chirality descriptor, CPL is crucial to the study of chirality generation, transfer, amplification, and regulation. However, due to the lack of transient characterization, high requirements for sample Stocks displacement, weak signal, few types, and high prices of existing CPL detection equipment, the lack of characterization technology for measuring chiral transfer and amplification processes has seriously restricted the development and application of chiral functional materials. Therefore, a circularly polarized luminescence detection device and detection method that can solve the above problems are urgently needed. Summary of the invention

[0004] The main purpose of the present invention is to provide a circularly polarized luminescence detection device and detection method with simple operation and accurate signals.

[0005] To achieve the above object, the technical solution of the present invention is:

[0006] A circularly polarized luminescence detection device comprises an excitation light source, a sample slot is provided at the output end of the excitation light source, a first lens is provided between the sample slot and the excitation light source, a photoelastic regulator is provided at the output end of the sample slot, the photoelastic regulator comprises a second lens and a linear polarizer, a circular polarizer which can perform left-hand rotation or right-hand rotation is provided between the second lens and the linear polarizer, a photon detector is provided at the output end of the photoelastic regulator, and the circular polarizer can be adjusted so that the photon detector detects light intensity.

[0007] According to the first aspect of the present invention, the photoelastic regulator can adjust the fast and slow axes of the quarter wave plate by left-rotating or right-rotating the circular polarizer so that the circularly polarized light is incident on the linear polarizer at 50 Hz.

[0008] According to the first aspect of the present invention, the transmission axis of the linear polarizer is 45°.

[0009] According to the first aspect of the present invention, the second lens is located between the sample slot and the circular polarizer, the first lens is located on the exit light path of the excitation light source, and the second lens is located on the exit light path of the sample slot.

[0010] According to a second aspect of the present invention, the method for detecting by the circularly polarized luminescence detection device comprises the following steps:

[0011] 1) For the luminous photons emitted by the excitation light source, the left-handed light or the right-handed light is filtered by the left-handed or right-handed circular polarizer respectively, and the polarization lifetime of the single wavelength photons is tested;

[0012] 2) According to the asymmetric factor formula g count =2(σ + -σ - ) / (σ + +σ - ) Dynamic monitoring of photon spin polarization and decay quenching process, realizing microscopic transient chiral optical signal detection from picoseconds to nanoseconds;

[0013] 3) Analyze the difference in luminescence lifetime of left-handed or right-handed circular polarizers from a macroscopic steady-state level;

[0014] 4) Through formula g decay =2(τ L -τ R ) / (τ L +τ R ) Calculate the luminescence lifetime asymmetry factor of the circular polarizer to provide parameters for quantitatively describing chiral transfer and amplification.

[0015] According to the second aspect of the present invention, in step 2), σ + is the instantaneous photon intensity of the left-handed circular polarizer, σ - is the instantaneous photon intensity of the right-handed circular polarizer.

[0016] According to the second aspect of the present invention, in step 4), τ L is + Detect the data difference after curve fitting, τ R is - Detect the difference in data after curve fitting.

[0017] One of the above technical solutions of the present invention has at least one of the following advantages or beneficial effects:

[0018] The present invention arranges a photoelastic regulator at the output end of the sample slot and the front end of the photon detector, so that the fast and slow axes of the quarter-wave plate can be adjusted to switch the left-handed or right-handed circularly polarized light to pass through the linear polarizer respectively, and the light is further converted into linear polarized light. Then, the circularly polarized light intensity can be obtained by comparing the intensity difference of the left-handed or right-handed circularly polarized light. The operation is simple and the signal is accurate. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] The present invention will be further described below in conjunction with the accompanying drawings and embodiments;

[0020] Attached Figure 1 It is an overall schematic diagram of an embodiment of the present invention;

[0021] Attached Figure 2 A schematic diagram of a left-handed or right-handed circular polarizer according to an embodiment of the present invention;

[0022] Attached Figure 3 A physical diagram of an embodiment of the present invention;

[0023] Attached Figure 4 A wavelength comparison diagram of an existing device according to an embodiment of the present invention and the present invention;

[0024] Attached Figure 5 A dynamic monitoring diagram of photon spin polarization and decay quenching process according to an embodiment of the present invention;

[0025] Attached Figure 6 A reference diagram of an existing detection device according to an embodiment of the present invention;

[0026] Attached Figure 7 This is a core optical path diagram of an existing detection device according to an embodiment of the present invention. DETAILED DESCRIPTION

[0027] The embodiments of the present invention are described in detail below, and examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals represent the same or similar elements or elements having the same or similar functions from beginning to end. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and cannot be understood as limiting the present invention.

[0028] In the description of the present invention, it should be understood that descriptions involving orientations, such as up, down, front, back, left, right, etc., and orientations or positional relationships indicated are based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present invention.

[0029] In the description of the present invention, "several" means one or more, "more" means more than two, "greater than", "less than", "exceed" etc. are understood as not including the number itself, and "above", "below", "within" etc. are understood as including the number itself. If there is a description of "first" or "second", it is only used for the purpose of distinguishing the technical features, and cannot be understood as indicating or implying the relative importance or implicitly indicating the number of the indicated technical features or implicitly indicating the order of the indicated technical features.

[0030] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, the features defined as "first" and "second" may explicitly or implicitly include one or more features.

[0031] In the description of the present invention, it should be noted that, unless otherwise clearly stipulated and limited, the term "connection" should be understood in a broad sense. For example, it can be a fixed connection or a movable connection, a detachable connection or a non-detachable connection, or an integral connection; it can be a mechanical connection, an electrical connection, or mutual communication; it can be a direct connection, or an indirect connection through an intermediate medium, and it can be internal connection between two elements, indirect connection, or an interactive relationship between two elements.

[0032] The following disclosure provides many different embodiments or examples for implementing different solutions of the present invention.

[0033] See attached Figure 1 To Attachment Figure 7 As shown, a circularly polarized luminescence detection device includes an excitation light source 1, a sample slot 2, a photoelastic regulator 4 and a photon detector 5 are provided at the output end of the excitation light source 1, a first lens 3 is provided between the sample slot 2 and the excitation light source 1, and the first lens 3 is located on the output light path of the excitation light source 1.

[0034] In one embodiment of the present invention, the photoelastic regulator 4 is disposed at the output end of the sample tank 2, and the photoelastic regulator 4 includes a second lens 41, a linear polarizer 42, and a circular polarizer 43 that can be left-handed or right-handed and is disposed between the second lens 41 and the linear polarizer 42. The photoelastic regulator 4 can adjust the fast and slow axes of the quarter-wave plate, and through the left-handed or right-handed circular polarizer 43, circularly polarized light enters the linear polarizer 42 at 50 Hz.

[0035] In one embodiment of the present invention, the transmission axis of the linear polarizer 42 is 45°, the second lens 41 is located between the sample tank 2 and the circular polarizer 43 , and the second lens 41 is located on the outgoing light path of the sample tank 2 .

[0036] In one embodiment of the present invention, the photon detector 5 is disposed at the output end of the photoelastic modulator 4, and the circular polarizer 43 can be adjusted so that the photon detector 5 detects the light intensity, and further by comparing the intensity difference of the left-handed or right-handed circularly polarized light, the circularly polarized light intensity ΔI=I L –I R , where I L with I R Represent the intensity of left-handed circularly polarized light and right-handed circularly polarized light, respectively.

[0037] For the existing CPL detection equipment and core optical path, please refer to the attached Figure 6 , Attachment Figure 7 , where the left side is the light source and the right side is the photon detector. It is found that there are factors such as lack of transient characterization, high requirements for sample Stocks displacement, weak signal, few types, and high price. The circular polarization luminescence detection device improves the ordinary fluorescence detector by using the physical circular dichroism transient absorption spectrum test method, and builds a circular polarization detection light path, which can achieve the following advantages (see attached Figure 4 ):

[0038] 1. Develop new precision detection technologies based on the polarization origin of circularly polarized luminescence and establish time-resolved spin-photon asymmetry analysis methods;

[0039] 2. Low cost: With the existing fluorescence instrument as the basic equipment, CPL testing can be achieved by only appropriately improving the optical path, which is much lower than the purchase cost of existing CPL equipment;

[0040] 3. Accurate signal: Taking chiral rare earth (Eu) complex as an example, the half width at half maximum (FWHM) of CPL is matched with the actual luminescence, but the FWHM measured by the existing JASCO CPL-300 is more than 20nm, which is inconsistent with the actual situation.

[0041] 4. Simple operation: Existing fluorescence detectors are widely available and can be operated skillfully by ordinary personnel. However, ultrafast spectroscopy has high requirements for operation. By improving the CPL detector based on the existing fluorescence spectrometer, the sample can be completed quickly.

[0042] In one embodiment of the present invention, referring to the attached Figure 4 , referring to the physical circular dichroism transient absorption spectrum test method, a circular polarization detection optical path is built to selectively detect the spin photons of the luminescent photon spin polarization and attenuation quenching process at the microscopic level, and its dynamic change process is analyzed according to the asymmetry factor formula (refer to the attached Figure 5 ), realizing the detection of microscopic transient chiral optical signals from picoseconds to nanoseconds.

[0043] The difference between left-handed and right-handed circularly polarized luminescence lifetimes is analyzed from a macroscopic steady-state level, and the circularly polarized luminescence lifetime asymmetry factor is calculated by formula, providing new parameters for quantitatively describing chiral transfer and amplification. The specific operation of the circularly polarized luminescence detection device includes the following steps:

[0044] 1. For the luminous photons emitted by the excitation light source 1, the left-handed light or the right-handed light is filtered by the left-handed or right-handed circular polarizer 43 respectively, and the polarization lifetime of the single wavelength photons is tested;

[0045] 2. According to the asymmetric factor formula g count =2(σ + -σ - ) / (σ ++σ - ) Dynamic monitoring of photon spin polarization and decay quenching process, realizing microscopic transient chiral optical signal detection from picoseconds to nanoseconds; (where σ + is the instantaneous online photon intensity of the left-handed circular polarizer 43, that is, the photon intensity at any abscissa time at this time; σ - is the instantaneous photon intensity of the right-hand circular polarizer 43, that is, the photon intensity at any abscissa time at that time. )

[0046] 3. Analyze the difference in luminescence lifetime of left-handed or right-handed circular polarizer 43 from the macroscopic steady-state level, and use the formula g decay =2(τ L -τ R ) / (τ L +τ R ) Calculate the asymmetric factor of the luminescence lifetime of the circular polarizer 43, and provide parameters for quantitatively describing chiral transfer and amplification. (where τ L is + Detect the data difference after curve fitting, τ R is - Detect the difference in data after curve fitting. )

[0047] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the claims and their equivalents.

Claims

1. A circularly polarized luminescence detection device, characterized in that: include: An excitation light source (1), wherein a sample slot (2) is provided at the output end of the excitation light source (1), and a first lens (3) is provided between the sample slot (2) and the excitation light source (1); A photoelasticity regulator (4), the photoelasticity regulator (4) being arranged at the output end of the sample tank (2), the photoelasticity regulator (4) comprising a second lens (41) and a linear polarizing plate (42), and a circular polarizing plate (43) capable of left-hand rotation or right-hand rotation being arranged between the second lens (41) and the linear polarizing plate (42); A photon detector (5) is arranged at the output end of the photoelasticity regulator (4), and the circular polarizer (43) can be adjusted so that the photon detector (5) detects light intensity.

2. The circularly polarized luminescence detection device according to claim 1, characterized in that: The photoelastic regulator (4) can adjust the fast and slow axes of the quarter wave plate, and by left-hand or right-hand rotation of the circular polarizer (43), circularly polarized light is incident on the linear polarizer (42) at 50 Hz.

3. The circularly polarized luminescence detection device according to claim 2, characterized in that: The transmission axis of the linear polarizing plate (42) is 45°.

4. The circularly polarized luminescence detection device according to claim 1, characterized in that: The second lens (41) is located between the sample slot (2) and the circular polarizer (43), the first lens (3) is located on the outgoing light path of the excitation light source (1), and the second lens (41) is located on the outgoing light path of the sample slot (2).

5. A method for detecting by the circularly polarized luminescence detection device according to claim 1, characterized in that: The steps include: 1) For the luminous photons emitted by the excitation light source (1), the left-handed light or the right-handed light is filtered by the left-handed or right-handed circular polarizer (43) respectively, and the polarization lifetime of the single-wavelength photons is tested; 2) According to the asymmetric factor formula g count =2(σ + -σ - ) / (σ + +σ - ) Dynamic monitoring of photon spin polarization and decay quenching process, realizing microscopic transient chiral optical signal detection from picoseconds to nanoseconds; 3) Analyzing the difference in luminescence lifetime of the left-handed or right-handed circular polarizer (43) from a macroscopic steady-state level; 4) Through formula g decay =2(τ L -τ R ) / (τ L +τ R ) calculates the luminescence lifetime asymmetry factor of the circular polarizer (43) to provide parameters for quantitatively describing chiral transfer and amplification.

6. The detection method according to claim 5, characterized in that: In step 2), σ + is the instantaneous photon intensity of the left-handed circular polarizer (43), σ - is the instantaneous photon intensity when the circular polarizer (43) is right-handed.

7. The detection method according to claim 5, characterized in that: In the step 4), τ L is + Detect the data difference after curve fitting, τ R is - Detect the difference in data after curve fitting.

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