Electrophoresis unit, component analyzer, and electrophoresis method

Abstract

The invention provides an electrophoresis unit, a component analyzer, and an electrophoresis method, which can make the pressure of a liquid applied to two ends of a capillary consistent through a simple mechanism. The electrophoresis unit (20) is provided with: a first flow path (30) having a first liquid level adjustment unit (38) that opens to the outside, adjusts the liquid level of a first liquid reservoir to a predetermined position, and makes the pressure of the liquid level in the first liquid reservoir equal to the external pressure; a second flow path (60) having a second liquid level adjustment unit (68) that opens to the outside, adjusts the liquid level of the second reservoir to a predetermined position, and makes the pressure of the liquid surface in the second reservoir equal to the external pressure; a capillary tube (24) communicating from the first reservoir (32) to the second reservoir (62); and electrodes (28) disposed inside the first flow path (30) and inside the second flow path (60), respectively, in which the positions (H) of the first liquid level adjustment unit (38) and the second liquid level adjustment unit (68) in the vertical direction are equal to each other.

Description

Technical Field

[0001] This invention relates to an electrophoresis unit, a component analysis device, and an electrophoresis method. Background Technology

[0002] For example, Japanese Patent Application Publication No. 2001-124736 discloses a capillary electrophoresis apparatus in which multiple capillaries are arranged at equal intervals parallel to the vertical on a plane, with the sample injection end immersed in the cathode buffer facing downwards, facilitating connection with the cathode buffer and the sample solution, ensuring that the liquid levels of the two buffers are consistent, and preventing the movement of the separation medium inside the capillary.

[0003] In addition, for example, Japanese Patent Application Publication No. 2020-128904 discloses a microchip electrophoresis apparatus in which buffer solutions in a first reservoir and a second reservoir are sequentially drawn from the surface side, the tips of the first suction nozzle and the second suction nozzle are lowered to a predetermined height while performing a suction action from above the first reservoir and the second reservoir, so that the liquid level in the first reservoir and the liquid level in the second reservoir are the same.

[0004] In addition, for example, Japanese Patent Application Publication No. 2016-24021 discloses an electrophoresis analysis chip, which includes: a first reservoir and a second reservoir disposed on a substrate; a swimming flow path connecting the first reservoir and the second reservoir and for swimming of a sample; and a liquid level adjustment unit capable of adjusting the liquid level of at least one of the liquid held in the first reservoir and the liquid held in the second reservoir. Summary of the Invention

[0005] The problem that the invention aims to solve

[0006] In existing technologies, it is necessary to make fine adjustments to the liquid levels at both ends of the capillary tube through adjustment mechanisms, which requires adjustment time and results in complex mechanisms and larger devices.

[0007] The purpose of this invention is to provide a technique that can make the pressure of the liquid applied to both ends of a capillary tube consistent through a simple mechanism.

[0008] Methods for solving problems

[0009] The electrophoresis unit of the first embodiment includes: a first flow path having a first liquid reservoir, a first inflow passage for liquid to flow into the first liquid reservoir, a first outflow passage for liquid to flow out of the first liquid reservoir, and a first liquid level adjustment section opening to the outside; a second liquid reservoir; a second flow path having a second inflow passage for liquid to flow into the second liquid reservoir, a second outflow passage for liquid to flow out of the second liquid reservoir, and a second liquid level adjustment section opening to the outside; a capillary connecting the first liquid reservoir to the second liquid reservoir; and electrodes respectively disposed inside the first flow path and inside the second flow path, wherein the vertical positions of the first liquid level adjustment section and the second liquid level adjustment section are equal to each other.

[0010] In one embodiment of the electrophoresis unit of the present invention, the vertical positions of the first liquid level adjustment section and the second liquid level adjustment section, which open to the outside, are equal to each other, so that the liquid levels accumulated in the first liquid reservoir and the second liquid reservoir are equal. Thus, the liquid pressure applied to both ends of the capillary can be made consistent through a simple mechanism. Attached Figure Description

[0011] Figure 1 This is a schematic structural diagram of the component analysis apparatus according to the first embodiment of the present invention.

[0012] Figure 2 This is a control flowchart of the control unit according to the first embodiment of the present invention.

[0013] Figure 3A This is a schematic diagram illustrating the situation where the liquid levels of the first liquid storage section and the second liquid storage section of the first embodiment of the present invention are consistent, and a diagram showing the situation where the electrophoretic liquid flows into the first liquid storage section.

[0014] Figure 3B then Figure 3A The diagram shows the situation where the capillary is filled with electrophoretic solution.

[0015] Figure 3C then Figure 3B , is a diagram showing the flow of the sample solution into the second reservoir.

[0016] Figure 3D then Figure 3C This is a diagram showing the situation where the sample solution is discharged from the second effluent passage and the electrophoresis solution is discharged from the first effluent passage.

[0017] Figure 3E then Figure 3D This is a diagram showing the situation where the liquid levels in the first and second liquid reservoirs are the same.

[0018] Figure 3F then Figure 3EThe figure shows the movement of components in a sample solution through a capillary tube via electrophoresis.

[0019] Figure 4 This is a schematic structural diagram of the component analysis apparatus according to the second embodiment of the present invention.

[0020] Figure 5 This is a control flowchart of the control unit according to the second embodiment of the present invention.

[0021] Figure 6A This is a schematic diagram illustrating the situation where the liquid levels of the first liquid storage section and the second liquid storage section of the second embodiment of the present invention are consistent, and a diagram showing the situation where the electrophoretic liquid flows into the first liquid storage section.

[0022] Figure 6B then Figure 6A , is a diagram showing the flow of the sample solution into the second reservoir.

[0023] Figure 6C then Figure 6B This is a diagram showing the situation where the liquid levels in the first and second liquid reservoirs are the same.

[0024] Figure 7 This is a schematic structural diagram of the component analysis apparatus according to the third embodiment of the present invention.

[0025] Figure 8 This is a control flow chart of the control unit according to the third embodiment of the present invention.

[0026] Figure 9A This is a schematic diagram illustrating the situation where the liquid levels of the first liquid storage section and the second liquid storage section of the third embodiment of the present invention are consistent, and a diagram showing the situation where the electrophoretic liquid flows into the first liquid storage section.

[0027] Figure 9B then Figure 9A , is a diagram showing the flow of the sample solution into the second reservoir.

[0028] Figure 9C then Figure 9B This is a diagram showing the situation where the sample solution is discharged from the second opening passage and the electrophoresis solution is discharged from the first opening passage.

[0029] Figure 9D then Figure 9C This diagram illustrates the situation where the sample solution is discharged from the second inflow passage and the electrophoresis solution is discharged from the first outflow passage, making the liquid levels of the first and second reservoirs the same.

[0030] Figure 9E then Figure 9D The figure shows the movement of components in a sample solution through a capillary tube via electrophoresis.

[0031] Figure 10 This is a schematic structural diagram of the component analysis apparatus according to the fourth embodiment of the present invention.

[0032] Figure 11 This is a control flow chart of the control unit according to the fourth embodiment of the present invention.

[0033] Figure 12A This is a schematic diagram illustrating the situation where the liquid levels of the first liquid storage section and the second liquid storage section of the fourth embodiment of the present invention are consistent, and a diagram showing the situation where the electrophoretic solution flows into the first liquid storage section.

[0034] Figure 12B then Figure 12A , is a diagram showing the flow of the sample solution into the second reservoir.

[0035] Figure 12C then Figure 12B This is a diagram showing the situation where the sample solution is discharged from the second opening passage and the electrophoresis solution is discharged from the first opening passage.

[0036] Figure 12D then Figure 12C This is a diagram showing the situation where the liquid levels in the first and second liquid reservoirs are the same.

[0037] Figure 13 This is a control flow chart of the control unit according to the fifth embodiment of the present invention.

[0038] Figure 14A This is a schematic diagram illustrating the situation where the liquid levels of the first liquid storage section and the second liquid storage section of the fifth embodiment of the present invention are consistent, and a diagram showing the situation where the electrophoretic solution flows into the first liquid storage section.

[0039] Figure 14B then Figure 14A , is a diagram showing the flow of the sample solution into the second reservoir.

[0040] Figure 14C then Figure 14B This is a diagram showing the situation where the sample solution is discharged from the second opening passage and the electrophoresis solution is discharged from the first opening passage.

[0041] Figure 14D then Figure 14C This is a diagram showing the situation where the liquid levels in the second liquid storage section and the first liquid storage section are the same.

[0042] Figure 15 This is a schematic structural diagram of the component analysis apparatus according to the sixth embodiment of the present invention.

[0043] Figure 16This is a control flow chart of the control unit according to the sixth embodiment of the present invention.

[0044] Figure 17A This is a schematic diagram illustrating the situation where the liquid levels of the first liquid storage section and the second liquid storage section of the sixth embodiment of the present invention are consistent, and a diagram showing the situation where the electrophoretic liquid flows into the first liquid storage section.

[0045] Figure 17B then Figure 17A , is a diagram showing the flow of the sample solution into the second reservoir.

[0046] Figure 17C then Figure 17B This is a diagram showing the situation where the sample solution is discharged from the second opening passage and the electrophoresis solution is discharged from the first opening passage.

[0047] Figure 17D then Figure 17C This is a diagram showing the situation where the liquid levels in the first and second liquid reservoirs are the same. Detailed Implementation

[0048] Hereinafter, an example of an embodiment of the present invention will be described with reference to the accompanying drawings. Furthermore, in each drawing, the same or equivalent constituent elements and components are labeled with the same reference numerals. Additionally, the scale of the drawings is exaggerated for ease of explanation and may sometimes differ from the actual scale. Also, in each drawing, the component analysis apparatus is viewed from the front, with arrow U positioned at the top, arrow D at the bottom, arrow R at the right side, arrow L at the left side, arrow B at the inside, and arrow F at the near-front side.

[0049] Furthermore, in the following description, the operator refers to the person who uses a capillary electrophoresis analysis device to analyze the components contained in the sample solution.

[0050] [First Implementation Method]

[0051] Appropriate reference Figures 1 to 3F This describes the first embodiment of the technology of the present invention.

[0052] (structure)

[0053] Figure 1 This is a schematic structural diagram of the capillary electrophoresis analysis apparatus 10 according to the first embodiment of the present invention. Figure 1 As shown, the capillary electrophoresis analysis apparatus 10 includes a first supply unit 44, a second supply unit 74, an electrophoresis unit 20, and a control unit 22. Furthermore, the capillary electrophoresis analysis apparatus 10 is an example of a "component analysis apparatus" according to this embodiment.

[0054] The first supply unit 44 is a device that supplies the electrophoresis solution RS used in the capillary electrophoresis analysis apparatus 10 of this embodiment to the first inflow passage 34 of the electrophoresis unit 20, and is controlled by the control unit 22 described later. The first supply unit 44 can be any device as long as it can supply the amount of electrophoresis solution RS desired by the operator; examples include pumps such as peristaltic pumps and drip devices such as electric pipettes. Furthermore, the electrophoresis solution RS can be any liquid, such as phosphate-buffered saline, which has a buffering effect on the components contained in the sample solution SS. Additionally, the first supply unit 44 can also be controlled by providing a valve (not shown) between it and the first inflow passage 34 described later to control the supply of the electrophoresis solution RS.

[0055] The second supply unit 74 is a device that supplies the sample solution SS analyzed by the capillary electrophoresis analysis apparatus 10 of this embodiment to the second inflow passage 64 of the electrophoresis unit 20, and is controlled by the control unit 22 described later. That is, the second supply unit 74 can be configured to have the same structure as the first supply unit 44, except for the type of liquid supplied. In addition, the sample solution SS can be any liquid; for example, a biological sample containing blood components can be used. Furthermore, the second supply unit 74 can also be configured to control the supply of the sample solution SS by providing a valve (not shown) between it and the second inflow passage 64 described later.

[0056] Furthermore, the first supply unit 44 and the second supply unit 74 are examples of "supply units" in this embodiment. Additionally, the sample solution SS and the electrophoresis solution RS are examples of "liquids" in this embodiment.

[0057] like Figure 1 As shown, the electrophoresis unit 20 includes a first flow path 30, a second flow path 60, a capillary 24, a detector 26, and a pair of electrodes 28.

[0058] like Figure 1 As shown, the first flow path 30 has a first liquid storage section 32 and a first outflow passage 36 that opens outward from the side wall of the first liquid storage section 32. In addition, a first flow path valve 40 is provided on the side of the first outflow passage 36 opposite to the first liquid storage section 32.

[0059] like Figure 1 As shown, the first liquid reservoir 32 is a recessed portion with a circular bottom surface in the first flow path 30. The upper side of the first liquid reservoir 32 serves as the first inflow passage 34 into which the electrophoresis solution RS flows. Furthermore, one end of the capillary tube 24 (described later) is connected to the opening on the bottom side of the first liquid reservoir 32. It should be noted that in this embodiment, the shape of the first liquid reservoir 32 is not limited to a circular bottom surface; the bottom surface can also be polygonal. Moreover, the bottom side and the upper side (i.e., the first inflow passage 34) can also be provided with different shapes.

[0060] In addition, such as Figure 1 As shown, one of a pair of electrodes 28 is disposed in the first liquid reservoir 32. The electrode 28 is subjected to voltage by the control unit 22 during capillary electrophoresis analysis, which will be described later.

[0061] The first outflow passage 36 is the portion connecting the first liquid storage section 32 and the exterior of the electrophoresis unit 20. By extending laterally within the vertical direction of the first liquid storage section 32, liquid can flow from the first liquid storage section 32 to the exterior of the electrophoresis unit 20. In other words, as... Figure 1 As shown, the first outflow passage 36 opens outward in a horizontal direction from the side wall of the first liquid storage section 32. Additionally, at the outer end of the first outflow passage 36 ( Figure 1 A first flow path valve 40 is provided on the front side of the first liquid storage section 32, which can prevent liquid from flowing to the outside through the first outflow passage 36.

[0062] In addition, such as Figure 1 As shown, the upper side of the first inflow passage 34 in this embodiment is open to the outside. In other words, in the first liquid storage section 32 of this embodiment, the electrophoresis liquid RS can be made to flow in from above the first flow path 30 using a pipette or other dripping device.

[0063] Furthermore, the first flow path 30 is an example of the "first flow path" involved in this embodiment. That is, the first liquid storage unit 32, the first inflow passage 34, the first outflow passage 36, and the first flow path valve 40 in this embodiment are respectively an example of the "first liquid storage unit", the "first inflow passage", the "first outflow passage", and the "valve that stops the flow of liquid".

[0064] like Figure 1 As shown, the second flow path 60 has a second liquid storage section 62 and a second outflow passage 66 that opens to the outside from the side wall of the second liquid storage section 62. In addition, a second flow path valve 70 is provided on the side of the second outflow passage 66 opposite to the second liquid storage section 62.

[0065] That is, the second flow path 60 is a portion formed on the opposite side of the first flow path 30, separated from it by the capillary tube 24, and has the same shape as the first flow path 30. In other words, the second flow path 60, the second liquid reservoir 62, the second inflow passage 64, the second outflow passage 66, and the second flow path valve 70 are respectively components of the first flow path 30, the first liquid reservoir 32, the first inflow passage 34, the first outflow passage 36, and the first flow path valve 40.

[0066] Furthermore, the second flow path 60 is an example of the "second flow path" in this embodiment. That is, the second liquid storage section 62, the second inflow passage 64, the second outflow passage 66, and the second flow path valve 70 in this embodiment are examples of the "second liquid storage section", "second inflow passage", "second outflow passage", and "valve that prevents liquid flow", respectively.

[0067] like Figure 1 As shown, the capillary 24 is a tubular portion connecting the first liquid reservoir 32 and the second liquid reservoir 62. The capillary 24 is not limited in shape as long as its diameter is thin enough to produce a capillary effect in the sample solution SS and the electrophoresis solution RS. For example, it can be a circle with a diameter of 25 μm to 100 μm, or a rectangle with sides of 25 μm to 100 μm. Furthermore, the capillary 24 in this embodiment is formed of a transparent material such as glass or acrylic (i.e., allowing light to pass through).

[0068] The detector 26, controlled by the control unit 22, is a device for detecting the components contained in the liquid located inside the capillary 24. As an example, the detector 26 can be a spectrophotometer, which measures the amount of light emitted from a light source not shown and transmitted through the capillary 24, thereby measuring the absorbance of the liquid located inside the capillary 24.

[0069] It should be noted that, in this embodiment, when the sample solution SS flows into the second reservoir 62 and the liquid level LH ​​(the vertical position H of the liquid surface in the liquid) is higher upstream than the outflow passages 36 and 66, the second flow path valve 70 is opened, and the sample solution SS flows outward to the vertical position H of the second outflow passage 66. Furthermore, in this embodiment, when the electrophoresis solution RS flows into the first reservoir 32 and the downstream liquid level LH ​​is higher than the outflow passages 36 and 66, the first flow path valve 40 is opened, and the electrophoresis solution RS flows outward to the vertical position H of the first outflow passage 36. In other words, the first outflow passage 36 opens outward to adjust the liquid level LH ​​of the electrophoresis solution RS in the first reservoir 32 to a predetermined position, i.e., position H, and to make the pressure of the liquid surface in the first reservoir 32 equal to the external pressure. In addition, the second outflow passage 66 opens to the outside to adjust the liquid level LH ​​of the sample solution SS in the second liquid storage section 62 to a predetermined position, namely position H, and to make the pressure of the liquid surface in the second liquid storage section 62 equal to the external pressure.

[0070] In addition, such as Figure 1As shown in Figure 3, in this embodiment, the vertical positions H of the second outflow passage 66 and the first outflow passage 36 are equal. Therefore, with the second flow path valve 70 and the first flow path valve 40 open, the liquid level LH ​​of the sample solution SS flowing into the second liquid storage section 62 is equal to the liquid level LH ​​of the electrophoresis solution RS flowing into the first liquid storage section 32. That is, in this embodiment, the second outflow passage 66 is an example of a "second liquid level adjustment section", and the first outflow passage 36 is an example of a "first liquid level adjustment section".

[0071] Furthermore, the electrophoresis unit 20 in this embodiment can be detachable and replaceable within the capillary electrophoresis analysis apparatus 10, or it can be configured to be installed and fixed to the capillary electrophoresis analysis apparatus 10. In either case, the first liquid level adjustment unit 38 and the second liquid level adjustment unit 68 are both configured such that their vertical positions H are equal when the electrophoresis unit 20 is installed in the capillary electrophoresis analysis apparatus 10.

[0072] Furthermore, in the following description, without distinguishing between the first flow path 30 and the second flow path 60, reference numerals will be used together with flow paths 30 and 60 in the accompanying drawings. Similarly, without specifically distinguishing between the structures of the first flow path 30 and the second flow path 60, reference numerals will be used together with the following: flow path 30, flow path 60, liquid storage section 32, liquid storage section 62, inflow passage 34, inflow passage 64, outflow passage 36, outflow passage 66, liquid level adjustment section 38, liquid level adjustment section 68, flow path valve 40, flow path valve 70, and reference numerals in the accompanying drawings. Similarly, without specifically distinguishing between the first supply section 44 and the second supply section 74, reference numerals will be used together with the following reference numerals in the accompanying drawings.

[0073] The control unit 22 is a device that controls the various structures of the electrophoresis unit 20, the electrode 28, and the supply units 44 and 74. The control unit 22 can have any structure, but as an example, it is a device with a CPU, ROM, and RAM. The CPU reads and executes the program stored in the ROM to perform various control actions.

[0074] More specifically, when the sample solution SS flows into the second reservoir 62 and the electrophoresis solution RS flows into the first reservoir 32 and the inside of the capillary 24, the control unit 22 applies a predetermined voltage to the electrode 28, thereby causing the components in the sample solution SS to be electrophoresed from the second reservoir 62 to the first reservoir 32. Furthermore, the control unit 22 uses the detector 26 to perform absorbance analysis on the liquid flowing in the capillary 24, thereby determining the concentration and type of components in the sample solution SS flowing in the capillary 24.

[0075] However, in capillary electrophoresis analysis, it is generally preferred that the liquid levels LH at both ends of the capillary 24, namely the first reservoir 32 and the second reservoir 62, be equal. More specifically, it is preferable that the liquid levels of the electrophoretic solution RS stored in the first reservoir 32 and the sample solution SS stored in the second reservoir 62 are equal before electrophoresis.

[0076] Here, refer to Figures 2 to 3F The steps of the electrophoresis method in this embodiment will be explained. Additionally, Figures 3A to 3F The vertical positional relationship of each structure in flow path 30, flow path 60, flow path valve 40, flow path valve 70 and capillary tube 24 is indicated in the figure, and other structures are sometimes omitted.

[0077] (Electrophoresis method)

[0078] In this embodiment, the control unit 22 executes a program (not shown) sequentially. Figure 2 The steps are shown.

[0079] First, in step S102, as Figure 3A As shown, the control unit 22 drives the first supply unit 44 to introduce the electrophoresis solution RS into the first flow path 30. More specifically, as... Figure 3A As shown, the control unit 22 supplies electrophoretic solution RS until the liquid level LH ​​of the electrophoretic solution RS in the first reservoir 32 is higher than the first outflow passage 36. Furthermore, the control unit 22 stops driving the first supply unit 44 after the liquid level LH ​​of the electrophoretic solution RS is higher than the first outflow passage 36.

[0080] Next, in step S104, the control unit 22, as follows: Figure 3B As shown, the capillary tube 24 is filled from the first flow path 30 with electrophoretic solution RS. Furthermore, in this embodiment, the electrophoretic solution RS fills the capillary tube 24 through a capillary effect. In other words, the control unit 22 waits until the electrophoretic solution RS stored in the first reservoir 32 fills the capillary tube 24.

[0081] Next, in step S106, as Figure 3C As shown, the control unit 22 drives the second supply unit 74 to introduce the sample solution SS into the second flow path 60. More specifically, as... Figure 3C As shown, the control unit 22 supplies sample solution SS until the liquid level LH ​​of sample solution SS in the second reservoir 62 is higher than the second outflow passage 66. Furthermore, the control unit 22 stops driving the second supply unit 74 after the liquid level LH ​​of sample solution SS is higher than the second outflow passage 66.

[0082] Next, in step S108, as Figure 3D As shown, the control unit 22 opens the flow valves 40 and 70 provided in the outflow passages 36 and 66, and discharges the electrophoresis solution RS and the sample solution SS from each outflow passage 36 and 66.

[0083] Next, in step S110, as Figure 3E As shown, the control unit 22 remains idle until the electrophoretic solution RS and sample solution SS inside the flow paths 30 and 60 reach equal levels LH due to gravity. More specifically, the control unit 22 remains idle until the levels LH of the electrophoretic solution RS and sample solution SS accumulated in the reservoirs 32 and 62 are equal to the vertical position H of the outflow passages 36 and 66. It should be noted that the idle period of the control unit 22 can be predetermined. Alternatively, a level gauge (not shown) can be installed in the reservoirs 32 and 62, and the control unit 22 can determine the idle period based on the level LH ​​value obtained from the level gauge. In addition, if the viscosity of the electrophoretic solution RS or sample solution SS is high, instead of waiting until the levels LH reach equal levels due to gravity, compressed air can be supplied from the inflow passages 34 and 64 to forcibly discharge the electrophoretic solution RS or sample solution SS from the outflow passages 36 and 66. In this case, a pressure pump (not shown) is connected to inflow passage 34 and inflow passage 64.

[0084] Next, in step S112, as Figure 3F As shown, the control unit 22 applies a voltage to a pair of electrodes 28 disposed in the second flow path 60 and the first flow path 30 to initiate electrophoresis. Furthermore, the control unit 22 determines the concentration and type of components contained in the sample solution SS by measuring the composition of the fluid moving in the capillary 24 due to electroosmotic flow using a detector 26.

[0085] In this embodiment, the liquid levels LH at both ends of the capillary 24 are adjusted through the above steps. Specifically, step S102 in this embodiment is an example of "allowing liquid to flow in from the first inflow passage," step S106 is an example of "allowing liquid to flow in from the second inflow passage," and step S104 is an example of "filling the capillary with liquid." Furthermore, step S110 in this embodiment is an example of "making the vertical positions of the liquid levels in the first and second liquid reservoirs equal, i.e., an isobaric step," and step S112 is an example of "performing electrophoresis of the components contained in the liquid inside the capillary."

[0086] It should be noted that in step S112, the concentration and type of components contained in the sample solution SS can be arbitrary. That is, the electrophoresis method described in this embodiment may not include the step of component analysis.

[0087] According to the capillary electrophoresis analysis apparatus 10, electrophoresis unit 20 and electrophoresis method of this embodiment, the following effects and benefits can be obtained.

[0088] (Functions and Effects)

[0089] The electrophoresis unit 20 of this embodiment includes: a first flow path 30 having a first liquid level adjustment section 38 that opens to the outside and makes the pressure of the first liquid reservoir 32 equal to the external pressure; and a second flow path 60 having a second liquid level adjustment section 68 that opens to the outside and makes the pressure of the second liquid reservoir 62 equal to the external pressure. Furthermore, in the electrophoresis unit 20 of this embodiment, the vertical positions H of the first liquid level adjustment section 38 and the second liquid level adjustment section 68 are equal to each other.

[0090] In the electrophoresis unit 20 of this embodiment, the vertical positions H of the first liquid level adjustment section 38 and the second liquid level adjustment section 68, which open to the outside, are equal to each other, so the liquid levels LH accumulated in the first liquid storage section 32 and the second liquid storage section 62 are equal. Therefore, according to the electrophoresis unit 20 of this embodiment, the pressure of the liquid applied to both ends of the capillary 24 can be made consistent through a simple mechanism.

[0091] Furthermore, in this embodiment, the electrophoresis unit 20 functions as a liquid level adjustment unit 38, 68, which is oriented laterally within the vertical direction of the liquid storage sections 32, 62 via the outflow passages 36, 66. This allows for the equalization of the liquid levels LH on both sides of the capillary 24 through a simple mechanism, and also ensures that the pressure of the liquid applied to both ends of the capillary 24 is consistent.

[0092] Furthermore, the electrophoresis unit 20 of this embodiment also includes a detector 26 for detecting the composition of the liquid flowing inside the capillary 24. Thus, according to the electrophoresis unit 20 of this embodiment, by including the detector 26, the composition of the sample solution SS flowing inside the capillary 24 can be detected.

[0093] Furthermore, the capillary electrophoresis analysis apparatus 10 of this embodiment includes a control unit 22, an electrophoresis unit 20, and supply units 44 and 74 for supplying sample solution SS to the electrophoresis unit 20. Therefore, according to the capillary electrophoresis analysis apparatus 10 of this embodiment, the pressure of the liquid applied to both ends of the capillary 24 is made uniform through a simple mechanism, thus improving the accuracy of component analysis based on capillary electrophoresis.

[0094] Furthermore, the electrophoresis method of this embodiment includes the step of allowing the electrophoretic solution RS to flow from the first inflow passage 34 into the first liquid storage section 32 of the first flow path 30. The first flow path 30 has a first liquid level adjustment section 38 that opens to the outside, making the pressure in the first liquid storage section 32 equal to the external pressure, and whose vertical position H is equal to that of the second liquid level adjustment section 68. Additionally, the electrophoresis method of this embodiment includes the step of allowing the sample solution SS to flow from the second inflow passage 64 into the second liquid storage section 62 of the second flow path 60, which has a second liquid level adjustment section 68 that opens to the outside, making the pressure in the second liquid storage section 62 equal to the external pressure. Furthermore, the electrophoresis method of this embodiment includes the steps of: filling the capillary 24, which extends from the first liquid storage section 32 to the second liquid storage section 62, with the electrophoretic solution RS; and using the first liquid level adjustment section 38 and the second liquid level adjustment section 68 to make the vertical position H of the liquid levels in the first liquid storage section 32 and the second liquid storage section 62 equal, i.e., an isobaric step. In addition, the electrophoresis method of this embodiment includes the step of using electrodes 28 disposed inside the first flow path 30 and the second flow path 60 to electrophore the components contained in the sample solution SS inside the capillary 24.

[0095] In the electrophoresis method of this embodiment, since the first liquid level adjustment section 38 and the second liquid level adjustment section 68, whose vertical positions H are equal, open to the outside, the liquid levels LH accumulated in the first liquid reservoir 32 and the second liquid reservoir 62 are equal. Therefore, according to the electrophoresis method of this embodiment, the pressure of the sample solution SS and the electrophoresis solution RS applied to both ends of the capillary 24 can be made consistent through a simple mechanism, improving the accuracy of electrophoresis of the components contained in the sample solution SS.

[0096] Furthermore, in the step of making the vertical position H of the liquid surface in the liquid equal in the electrophoresis method of this embodiment, the liquid is allowed to flow out from the outflow passages 36 and 66, which function as level adjustment units 38 and 68, in the vertical direction within the range of the liquid storage sections 32 and 62, thereby adjusting the vertical position H of the liquid surface in the liquid stored in the liquid storage sections 32 and 62. Therefore, by employing the electrophoresis method of this embodiment, the liquid levels LH on both sides of the capillary 24 can be aligned using the outflow passages 36 and 66, which function as level adjustment units 38 and 68, and the pressure of the liquid applied to both ends of the capillary 24 can be made consistent.

[0097] Furthermore, the electrophoresis method of this embodiment includes a step of analyzing the internal components of the capillary 24 using a detector 26 that detects the components of the liquid flowing in the capillary 24. Therefore, according to the electrophoresis method of this embodiment, the pressure of the liquid applied to both ends of the capillary 24 is made uniform through a simple mechanism, thus improving the accuracy of component analysis based on capillary electrophoresis. In other words, according to the electrophoresis method of this embodiment, even if the amounts of sample solution SS and electrophoretic solvent RS introduced into the first reservoir 32 and the second reservoir 62 are different, precise control is not required, and analysis can be performed by electrophoresis.

[0098] Next, refer to Figures 4 to 6C The capillary electrophoresis analysis apparatus 110 according to the second embodiment of the present invention will be described. Furthermore, the same structural reference numerals as those in the first embodiment are used in the capillary electrophoresis analysis apparatus 110 according to the second embodiment, and specific descriptions are omitted.

[0099] [Second Implementation]

[0100] (structure)

[0101] In this embodiment, such as Figure 4 As shown, the shapes of the inflow passages 134 and 164, the outflow passages 136 and 166, and the liquid storage sections 132 and 162 are different from those in the first embodiment.

[0102] In this embodiment, such as Figure 4 As shown, inflow passages 134 and 164 are tubular liquid passages extending vertically. Furthermore, upstream of inflow passages 134 and 164 in the capillary electrophoresis analysis apparatus 110, flow path valves 140 and 170 are provided at the open ends of flow paths 130 and 160. Electrophoresis solution RS or sample solution SS supplied from supply units 44 and 74 flows into inflow passages 134 and 164 through flow path valves 140 and 170.

[0103] In addition, in this embodiment, such as Figure 4 As shown, the outflow passages 136 and 166 extend horizontally. In this embodiment, the side of the outflow passages 136 and 166 opposite to the liquid storage portions 132 and 162 opens outside the flow paths 130 and 160, and the pressure at the opening is equal to the external air pressure outside the flow paths 130 and 160. In other words, the pressure at the downstream opening of the outflow passages 136 and 166 is equal to the external air pressure.

[0104] Furthermore, in this embodiment, such as Figure 4As shown, the liquid storage sections 132 and 162 extend horizontally from the downstream side of the inflow passages 134 and 164 to the upstream side of the outflow passages 136 and 166, and extend upward on the downstream side. In other words, the liquid storage sections 132 and 162 in this embodiment are the bottom portions of the flow paths 130 and 160, which are generally U-shaped. In addition, the capillary tube 124 connects the liquid storage sections 132 and 162 to each other.

[0105] In this embodiment, the electrophoresis solution RS or sample solution SS is supplied from the supply units 44 and 74 to the inflow passages 134 and 164, thereby flowing out to the outside sequentially through the inflow passages 134 and 164, the storage units 132 and 162, and the outflow passages 136 and 166. Furthermore, in this embodiment, when the electrophoresis solution RS or sample solution SS is supplied from the supply units 44 and 74 to the inflow passages 134 and 164, any portion of the inflow passages 134 and 164, and the storage units 132 and 162, is filled with the electrophoresis solution RS or sample solution SS. Additionally, in this embodiment, the liquid level of the electrophoresis solution RS or sample solution SS may be located midway through the outflow passages 136 and 166. In other words, the outflow passages 136 and 166 may not be completely filled with the electrophoresis solution RS or sample solution SS.

[0106] Furthermore, in this embodiment, when the flow path valves 140 and 170 are closed, the flow of liquid within the inflow passages 134 and 164 is stopped. More specifically, with the flow path valves 140 and 170 closed, external gas will not flow into the inflow passages 134 and 164 from the outside, therefore the flow of liquid within the flow paths 130 and 160 stops (see also the following description). Figure 6B wait).

[0107] Furthermore, in this embodiment, the vertical positions H of the first outflow passage 136 and the second outflow passage 166 are also as described later. Figure 6B As shown, they are equal. Therefore, in this embodiment, the first outflow passage 136 also becomes the first liquid level adjustment unit 138, and the second outflow passage 166 also becomes the second liquid level adjustment unit 168. That is, in this embodiment, the first outflow passage 136 is also an example of a "first liquid level adjustment unit", and the second outflow passage 166 is also an example of a "second liquid level adjustment unit". In addition, other structures are the same as in the first embodiment.

[0108] Next, refer to Figures 5 to 6C The steps of the electrophoresis method in this embodiment will be explained. Furthermore, in Figure 6C The diagram shows the vertical positional relationship of each structure in flow paths 130, 160, flow valves 140, 170, and capillary tube 124, with other structures sometimes omitted.

[0109] (Electrophoresis method)

[0110] In this embodiment, the control unit 22 executes a program (not shown) sequentially. Figure 5 The steps are shown.

[0111] First, in step S202, as Figure 6A As shown, the control unit 22 drives the first supply unit 44 to introduce the electrophoresis liquid RS into the first flow path 130, and fills the first inflow passage 134, the first storage unit 132 and the first outflow passage 136 with the electrophoresis liquid RS.

[0112] Next, in step S204, the control unit 22 fills the capillary 124 from the first flow path 130 using the electrophoretic solution RS. Furthermore, in this embodiment, the electrophoretic solution RS fills the capillary 124 through a capillary effect. In other words, the control unit 22 waits until the electrophoretic solution RS stored in the first reservoir 132 fills the capillary 124.

[0113] Next, in step S206, the control unit 22 stops the drive of the first supply unit 44 and closes the first flow path valve 140.

[0114] Next, in step S208, the control unit 22, as follows: Figure 6B As shown, the second supply unit 74 is driven to introduce the sample solution SS into the second flow path 160, and the sample solution SS fills the second inflow passage 164, the second storage unit 162 and the second outflow passage 166.

[0115] Next, in step S210, as Figure 6C As shown, the control unit 22 stops the drive of the second supply unit 74 and closes the second flow path valve 170. Furthermore, in this embodiment, with the flow path valves 140 and 170 closed, as... Figure 6C As shown, the liquid levels LH of the electrophoresis solution RS and the sample solution SS are equal.

[0116] Next, in step S212, the control unit 22 applies a voltage to a pair of electrodes 28 disposed in the second flow path 160 and the first flow path 130 to start electrophoresis. In addition, the control unit 22 determines the concentration and type of components contained in the sample solution SS by using the detector 26 to measure the composition of the fluid moving in the capillary 124 due to electroosmosis.

[0117] In this embodiment, the liquid levels LH at both ends of the capillary 124 are adjusted through the above steps. Specifically, step S202 in this embodiment is an example of "allowing liquid to flow in from the first inflow passage," step S208 is an example of "allowing liquid to flow in from the second inflow passage," and step S204 is an example of "filling the capillary with liquid." Furthermore, steps S206 and S210 in this embodiment are examples of "ordering the vertical positions of the liquid surfaces in the first and second liquid reservoirs to be equal, i.e., isobaric order," and step S212 is an example of "ordering the components contained in the liquid inside the capillary to undergo electrophoresis."

[0118] The component analysis apparatus and electrophoresis method of this embodiment can achieve the following effects and benefits.

[0119] (Functions and Effects)

[0120] The electrophoresis unit 120 of this embodiment has flow path valves 140 and 170 in the inflow passages 134 and 164 to prevent the flow of liquid into the reservoirs 132 and 162 from above. Therefore, by stopping the flow of liquid into the passages 134 and 164 through the flow path valves 140 and 170, the liquid level in the inflow passages 134 and 164 does not affect the pressure of the liquid in the reservoirs 132 and 162. In other words, the pressure of the liquid applied to the reservoirs 132 and 162 depends on the external pressure and the liquid level in the outflow passages 136 and 166; therefore, the pressure of the liquid applied to the second reservoir 162 is equal to the pressure of the liquid applied to the first reservoir 132.

[0121] Therefore, according to the electrophoresis unit 120 of this embodiment, without discharging the liquid inside the flow path 130, 160 in the liquid storage section 132, 162 from the inflow passage 134, 164, the pressure of the liquid applied to both ends of the capillary 124 can be made consistent.

[0122] Furthermore, in the isobaric step of the electrophoresis method of this embodiment, flow path valves 140 and 170 are used to stop the flow of liquid into the reservoirs 132 and 162 of the inflow passages 134 and 164 from above, thereby stopping the flow of liquid inside the inflow passages 134 and 164. Therefore, stopping the flow of liquid into the passages 134 and 164 by the flow path valves 140 and 170 does not affect the pressure of the liquid in the reservoirs 132 and 162. In other words, the pressure of the liquid applied to the reservoirs 132 and 162 depends on the external pressure and the liquid height in the outflow passages 136 and 166, so the pressure of the liquid applied to the second reservoir 162 is equal to the pressure of the liquid applied to the first reservoir 132.

[0123] Therefore, according to the electrophoresis method of this embodiment, without discharging the liquid inside the flow path 130, 160 in the liquid storage section 132, 162 from the inflow passage 134, 164, the pressure of the liquid applied to both ends of the capillary 124 can be made consistent.

[0124] Furthermore, in the above description, flow path valves 140 and 170 are provided in the capillary electrophoresis analysis apparatus 110, but are not limited thereto; the electrophoresis unit 120 may also have flow path valves 140 and 170. That is, flow path valves 140 and 170 may also be provided in the inflow passages 134 and 164.

[0125] Furthermore, in this embodiment, the same structure as in the first embodiment can also achieve the same function and effect as in the first embodiment.

[0126] Next, refer to Figures 7 to 10 The capillary electrophoresis analysis apparatus 210 according to the third embodiment of the present invention will be described. Furthermore, the same structural reference numerals as those in the first embodiment are used in the capillary electrophoresis analysis apparatus 210 according to the third embodiment, and specific descriptions are omitted.

[0127] [Third Implementation Method]

[0128] (structure)

[0129] In this embodiment, such as Figure 7 As shown, the shapes of the inflow passages 234 and 264, the outflow passages 236 and 266, and the liquid storage sections 232 and 262 are different from those of the first embodiment or the second embodiment.

[0130] In this embodiment, such as Figure 7 As shown, inflow passages 234 and 264 are tubular liquid passages extending horizontally. Furthermore, the side of inflow passages 234 and 264 opposite to the liquid storage sections 232 and 262 opens to the outside of flow paths 230 and 260, and the pressure at the opening is equal to the external air pressure outside flow paths 230 and 260. In other words, the pressure at the first opening in outflow passages 236 and 266 is equal to the external air pressure. In addition, in this embodiment, the vertical position H1 of inflow passages 234 and 264 is lower than the vertical position H2 of outflow passages 236 and 266 (also refer to the following description). Figures 9A to 9E wait).

[0131] In this embodiment, when electrophoresis solution RS or sample solution SS is supplied from supply units 44 and 74, inflow passages 234 and 264 are connected to tubes (not shown). Furthermore, when the supply of electrophoresis solution RS or sample solution SS from supply units 44 and 74 is stopped, the tubes (not shown) are removed from inflow passages 234 and 264, and inflow passages 234 and 264 open to the outside.

[0132] Furthermore, in this embodiment, such as Figure 7 As shown, outflow passages 236 and 266 extend vertically and branch horizontally. The portions branching from outflow passages 236 and 266 and extending horizontally are opening passages 246 and 276. That is, the first opening passage 246 is an example of the "first opening passage" in this embodiment, and the second opening passage 276 is an example of the "second opening passage" in this embodiment. Furthermore, flow path valves 240 and 270 are provided on the side of the opening passages 246 and 276 in the capillary electrophoresis analysis apparatus 210 opposite to the outflow passages 236 and 266. The side of the flow path valves 240 and 270 opposite to the flow paths 230 and 260 (the capillary electrophoresis analysis apparatus 210 side) opens to the outside. When the flow path valves 240 and 270 are open, the pressure at the opening end of the flow paths 230 and 260 is equal to the external air pressure. In other words, when the flow path valves 240 and 270 are open, the internal pressure of the open passages 246 and 276 is equal to the external air pressure.

[0133] Furthermore, in this embodiment, such as Figure 7 As shown, the liquid storage sections 232 and 262 extend horizontally from the downstream side of the inflow passages 234 and 264 to the upstream side of the outflow passages 236 and 266, and extend upward on the upstream side. In other words, the liquid storage sections 232 and 262 in this embodiment are the bottom portions of the flow paths 230 and 260, which are approximately U-shaped. Furthermore, capillary tubes 224 connect the liquid storage sections 232 and 262 to each other.

[0134] In this embodiment, the electrophoresis solution RS or the sample solution SS is supplied from the supply units 44 and 74 to the inflow passages 234 and 264, thereby flowing out to the outside sequentially through the inflow passages 234 and 264, the storage units 232 and 262, and the outflow passages 236 and 266. Furthermore, in this embodiment, when the electrophoresis solution RS or the sample solution SS is supplied from the supply units 44 and 74 to the inflow passages 234 and 264, any portion of the inflow passages 234 and 264, the storage units 232 and 262, and the outflow passages 236 and 266 is filled with the electrophoresis solution RS or the sample solution SS.

[0135] Furthermore, in this embodiment, when the flow path valves 240 and 270 are closed, the flow of liquid within the opening passages 246 and 276 is stopped. More specifically, with the flow path valves 240 and 270 closed, the opening passages 246 and 276 do not open to the outside, thus stopping the flow of liquid within the flow paths 230 and 260 (see also the description below). Figure 9B wait).

[0136] Furthermore, in this embodiment, the lower position H2 of the first opening passage 246 and the second opening passage 276 in the vertical direction is as described later. Figure 9B As shown, they are equal. Furthermore, in this embodiment, the lower vertical position H1 in the first inflow passage 234 and the second inflow passage 264 is as described later. Figure 9B As shown, they are equal. Furthermore, in this embodiment, the upper vertical position H2 of the first inflow passage 234 and the second inflow passage 264 is equal to the lower vertical position H2 of the first opening passage 246 and the second opening passage 276. In this embodiment, the first inflow passage 234 becomes the first liquid level adjustment unit 238, and the second inflow passage 264 becomes the second liquid level adjustment unit 268. That is, in this embodiment, the first inflow passage 234 is an example of a "first liquid level adjustment unit," and the second inflow passage 264 is an example of a "second liquid level adjustment unit." Other structures are the same as in the first embodiment.

[0137] Next, refer to Figures 8 to 9E The steps of the electrophoresis method in this embodiment will be explained. Furthermore, in Figures 9A to 9E The diagram shows the vertical positional relationship of each structure in flow paths 230, 260, flow valves 240, 270, and capillary tube 224, with other structures sometimes omitted.

[0138] (Electrophoresis method)

[0139] In this embodiment, the control unit 22 executes a program (not shown) sequentially. Figure 8 The steps are shown.

[0140] First, in step S302, as Figure 9A As shown, the control unit 22 drives the first supply unit 44 to introduce the electrophoresis liquid RS into the first flow path 230, and fills the first inflow passage 234, the first storage unit 232 and the first outflow passage 236 with the electrophoresis liquid RS.

[0141] Next, in step S304, the control unit 22 fills the capillary 224 from the first flow path 230 using the electrophoretic solution RS. Furthermore, in this embodiment, the electrophoretic solution RS fills the capillary 224 through a capillary effect. In other words, the control unit 22 waits until the electrophoretic solution RS stored in the first reservoir 232 fills the capillary 224.

[0142] Next, in step S306, the control unit 22, as follows: Figure 9B As shown, the second supply unit 74 is driven to introduce the sample solution SS into the second flow path 260, and the sample solution SS fills the second inflow passage 264, the second storage unit 262 and the second outflow passage 266.

[0143] Next, in step S308, as Figure 9C As shown, control unit 22 stops driving supply units 44 and 74 and opens flow path valves 240 and 270. Thus, in this embodiment, as... Figure 9C As shown, the liquid levels LH of the electrophoresis solution RS and the sample solution SS drop to position H2 in the vertical direction of the opening passages 246 and 276. More specifically, when the viscosity of the electrophoresis solution RS or the sample solution SS is low, after opening the flow valves 240 and 270, the liquid level LH ​​drops to position H2 on the lower side in the vertical direction of the opening passages 246 and 276 due to gravity. Alternatively, when the viscosity of the electrophoresis solution RS or the sample solution SS is high, instead of waiting until the liquid levels LH are equal due to gravity, compressed air can be supplied from the outflow passages 236 and 266 to forcibly discharge the electrophoresis solution RS or the sample solution SS from the inflow passages 234 and 264. In this case, the outflow passages 236 and 266 are connected to a pressure pump (not shown).

[0144] Next, in step S310, as Figure 9D As shown, the control unit 22 removes a tube (not shown) from the second inflow passage 264 and the first inflow passage 234, opening the inflow passages 234 and 264 to the outside. Here, in this embodiment, when the viscosity of the electrophoresis solution RS or the sample solution SS is high, such as... Figure 9D As shown, the liquid level of the electrophoresis solution RS or the sample solution SS is located inside the inflow passages 234 and 264, therefore the liquid level LH ​​is position H2 in the vertical direction of the inflow passages 234 and 264. In other words, when the viscosity of the electrophoresis solution RS or the sample solution SS is high, the liquid level LH ​​does not change even if the tube (not shown) is removed from the second inflow passage 264 and the first inflow passage 234. Furthermore, in this embodiment, when the viscosity of the electrophoresis solution RS or the sample solution SS is low, the liquid level LH ​​drops to position H1 in the lower vertical direction of the inflow passages 234 and 264 due to gravity.

[0145] That is, in this embodiment, in step S310, after the tube (not shown) is removed from the first inflow passage 234 and the second inflow passage 264, the system waits until it is confirmed that the height of the liquid level LH ​​no longer changes.

[0146] Next, in step S312, as Figure 9E As shown, the control unit 22 applies a voltage to a pair of electrodes 28 disposed in the second flow path 260 and the first flow path 230 to initiate electrophoresis. Furthermore, the control unit 22 determines the concentration and type of components contained in the sample solution SS by using a detector 26 to measure the composition of the fluid moving in the capillary 224 due to electroosmotic flow.

[0147] Furthermore, in this embodiment, such as Figure 9E As shown, if the vertical positions H1 of the inflow passages 234 and 264 are equal, and there are electrophoresis solution RS and sample solution SS liquid surfaces inside the inflow passages 234 and 264, then the lengths from the opening end to the liquid surface can also be inconsistent. For example, in Figure 9E In the second flow path 260, the length EB1 from the opening end to the liquid surface is shorter than the length EB2 from the opening end to the liquid surface in the first flow path 230.

[0148] In this embodiment, the liquid levels LH at both ends of the capillary 224 are adjusted through the above steps. Specifically, step S302 in this embodiment is an example of "allowing liquid to flow in from the first inflow passage," step S306 is an example of "allowing liquid to flow in from the second inflow passage," and step S304 is an example of "filling the capillary with liquid." Furthermore, steps S308 and S310 in this embodiment are examples of "ordering the vertical positions of the liquid surfaces in the first and second liquid reservoirs to be equal, i.e., isobaric order," and step S312 is an example of "ordering the components contained in the liquid inside the capillary to undergo electrophoresis."

[0149] The component analysis apparatus and electrophoresis method of this embodiment can achieve the following effects and benefits.

[0150] (Functions and Effects)

[0151] In this embodiment, the inflow passages 234 and 264 of the electrophoresis unit 220 allow liquid to flow from above into the storage portions 232 and 262, and open outwards to function as liquid level adjustment portions 238 and 268. Therefore, according to this embodiment, the electrophoresis unit 220 can achieve uniform liquid levels LH on both sides of the capillary 224 through a simple mechanism, and uniform pressure of the liquid applied to both ends of the capillary 224.

[0152] Furthermore, according to the electrophoresis unit 220 of this embodiment, even if air is mixed into the electrophoresis solution RS or sample solution SS flowing into the inflow passages 234 and 264, the air can be easily discharged from the inflow passages 234 and 264 during the isobaric step. Therefore, the electrophoresis unit 220 of this embodiment can maintain a consistent liquid level LH ​​regardless of the sealing performance of the upstream side of the inflow passages 234 and 264.

[0153] In the electrophoresis method of this embodiment, during the isobaric step, liquid flows into the reservoirs 232 and 262 from above, and flows out through the inflow passages 234 and 264, which open to the outside and function as level adjustment sections 238 and 268. Therefore, according to the electrophoresis method of this embodiment, the liquid levels LH on both sides of the capillary 224 can be made consistent using a simple mechanism, and the pressure of the liquid applied to both ends of the capillary 224 can be made consistent.

[0154] Furthermore, in the electrophoresis method of this embodiment, during the isobaric step, the liquid flows out from the inflow passages 234 and 264. Therefore, even if air is mixed into the electrophoresis solution RS or sample solution SS flowing into the inflow passages 234 and 264, the air can be easily discharged from the inflow passages 234 and 264. Thus, according to the electrophoresis method of this embodiment, the liquid level LH ​​can be kept consistent regardless of the sealing performance of the upstream side of the inflow passages 234 and 264.

[0155] Furthermore, in the above description, flow path valves 240 and 270 are provided in the capillary electrophoresis analysis apparatus 210, but are not limited thereto; the electrophoresis unit 220 may also have flow path valves 240 and 270. That is, flow path valves 240 and 270 may also be provided in the open passages 246 and 276.

[0156] Furthermore, in this embodiment, the same structure as the first or second embodiment can also achieve the same function and effect as the first or second embodiment.

[0157] Next, refer to Figures 10 to 12D The capillary electrophoresis analysis apparatus 310 according to the fourth embodiment of the present invention will be described. Furthermore, the same structural reference numerals as those in the first embodiment are used in the capillary electrophoresis analysis apparatus 310 according to the fourth embodiment, and specific descriptions are omitted.

[0158] [Fourth Implementation Method]

[0159] (structure)

[0160] In this embodiment, such as Figure 10As shown, the shapes of the inflow passages 334 and 364, the outflow passages 336 and 366, and the liquid storage sections 332 and 362 are different from those of the first embodiment or the second embodiment.

[0161] In this embodiment, such as Figure 10 As shown, inflow passages 334 and 364 are tubular liquid passages extending vertically. In addition, the side of inflow passages 334 and 364 opposite to the liquid storage sections 332 and 362 opens to the outside of flow paths 330 and 360, and the pressure at the opening end is equal to the external air pressure outside flow paths 330 and 360.

[0162] Furthermore, in this embodiment, the outflow passages 336 and 366 are formed with the same shape as the outflow passages 236 and 266 in the third embodiment. That is, in this embodiment, as... Figure 10 As shown, outflow passages 336 and 366 extend vertically and branch horizontally. Furthermore, flow path valves 340 and 370 are provided on the side of the opening passages 346 and 376 in the capillary electrophoresis analysis apparatus 310 opposite to the outflow passages 336 and 366. The side of the flow path valves 340 and 370 opposite to the flow paths 330 and 360 (the capillary electrophoresis analysis apparatus 310 side) opens to the outside. When the flow path valves 340 and 370 are open, the pressure at the opening end of the flow paths 330 and 360 is equal to the external air pressure. In other words, when the flow path valves 340 and 370 of the opening passages 346 and 376 are open, the pressure inside the opening passages 346 and 376 is equal to the external air pressure.

[0163] Furthermore, in this embodiment, such as Figure 10 As shown, the liquid storage sections 332 and 362 extend horizontally from the downstream side of the inflow passages 334 and 364 to the upstream side of the outflow passages 336 and 366, and extend upward on both the upstream and downstream sides. In other words, the liquid storage sections 332 and 362 in this embodiment are the bottom portions of the flow paths 330 and 360, which are approximately U-shaped. Furthermore, capillary tubes 324 connect the liquid storage sections 332 and 362 to each other.

[0164] In this embodiment, the electrophoresis solution RS or sample solution SS is supplied from the supply units 44 and 74 to the inflow passages 334 and 364, thereby flowing out to the outside sequentially through the inflow passages 334 and 364, the storage units 332 and 362, and the outflow passages 336 and 366. Furthermore, in this embodiment, when the electrophoresis solution RS or sample solution SS is supplied from the supply units 44 and 74 to the inflow passages 334 and 364, any portion of the inflow passages 334 and 364, the storage units 332 and 362, and the outflow passages 336 and 366 are filled with the electrophoresis solution RS or sample solution SS. Moreover, in this embodiment, as long as the liquid level of the electrophoresis solution RS or sample solution SS is located vertically above the opening passages 346 and 376, the liquid level may also be located midway through the outflow passages 336 and 366. In other words, the outflow passages 336 and 366 may not be completely filled with the electrophoresis solution RS or sample solution SS.

[0165] Furthermore, in this embodiment, when the flow path valves 340 and 370 are closed, the flow of liquid within the opening passages 346 and 376 is stopped. More specifically, when the flow path valves 340 and 370 are closed, the opening passages 346 and 376 do not open to the outside, thus stopping the flow of liquid within them (see also the description below). Figure 12B wait).

[0166] Furthermore, in this embodiment, the vertical positions H of the first opening passage 346 and the second opening passage 376 are as described later. Figure 12B As shown, they are equal. Furthermore, in this embodiment, the first opening passage 346 becomes the first liquid level adjustment unit 338, and the second opening passage 376 becomes the second liquid level adjustment unit 368. That is, in this embodiment, the first opening passage 346 is an example of a "first liquid level adjustment unit," and the second opening passage 376 is an example of a "second liquid level adjustment unit." Other structures are the same as in the first embodiment.

[0167] Next, refer to Figures 11 to 12D The steps of the electrophoresis method in this embodiment will be explained. Furthermore, in Figures 12A to 12D The diagram shows the vertical positional relationship of each structure in flow paths 330, 360, flow path valves 340, 370, and capillary tube 324, sometimes omitting other structures.

[0168] (Electrophoresis method)

[0169] In this embodiment, the control unit 22 executes a program (not shown) sequentially. Figure 11 The steps are shown.

[0170] First, in step S402, as Figure 12AAs shown, the control unit 22 drives the first supply unit 44 to introduce the electrophoresis liquid RS into the first flow path 330, and fill the first inflow passage 334, the first storage unit 332 and the first outflow passage 336 with the electrophoresis liquid RS.

[0171] Next, in step S404, the control unit 22 fills the capillary 324 from the first flow path 330 using the electrophoretic solution RS. Furthermore, in this embodiment, the electrophoretic solution RS fills the capillary 324 through a capillary effect. In other words, the control unit 22 waits until the electrophoretic solution RS stored in the first reservoir 332 fills the capillary 324.

[0172] Next, in step S406, the control unit 22, as follows: Figure 12B As shown, the second supply unit 74 is driven to introduce the sample solution SS into the second flow path 360, and the sample solution SS fills the second inflow passage 364, the second storage unit 362 and the second outflow passage 366.

[0173] Next, in step S408, as Figure 12C As shown, control unit 22 stops the drive of supply units 44 and 74, and opens flow path valves 340 and 370. Thus, as... Figure 12C As shown, the liquid levels LH of the electrophoresis solution RS and the sample solution SS decreased.

[0174] Next, in step S410, as Figure 12D As shown, the control unit 22 remains on standby until the levels LH of the electrophoretic solution RS and the sample solution SS inside the flow paths 330 and 360 become equal due to gravity. More specifically, the control unit 22 remains on standby until the levels LH of the electrophoretic solution RS and the sample solution SS stored in the liquid storage sections 332 and 362 are equal to the vertical positions H of the opening passages 346 and 376.

[0175] Next, in step S412, the control unit 22 applies a voltage to a pair of electrodes 28 disposed in the first flow path 330 and the second flow path 360 to start electrophoresis. In addition, the control unit 22 determines the concentration and type of components contained in the sample solution SS by using the detector 26 to measure the composition of the fluid moving in the capillary 324 due to electroosmosis.

[0176] In this embodiment, the liquid levels LH at both ends of the capillary 324 are adjusted through the above steps. Specifically, step S402 in this embodiment is an example of "allowing liquid to flow in from the first inflow passage," step S406 is an example of "allowing liquid to flow in from the second inflow passage," and step S404 is an example of "filling the capillary with liquid." Furthermore, steps S408 and S410 in this embodiment are examples of "ordering the vertical positions of the liquid surfaces in the first and second liquid reservoirs to be equal, i.e., isobaric order," and step S412 is an example of "ordering the components contained in the liquid inside the capillary to undergo electrophoresis."

[0177] The component analysis apparatus and electrophoresis method of this embodiment can achieve the following effects and benefits.

[0178] (Functions and Effects)

[0179] In this embodiment, the electrophoresis unit 320 functions as a liquid level adjustment unit 338, 368 by opening to the outside through opening passages 346, 376 branching from the outflow passages 336, 366. Therefore, according to the electrophoresis unit 320 of this embodiment, liquid flowing in the flow paths 330, 360 can be made to flow out through the outflow passages 336, 366 or the opening passages 346, 376.

[0180] Furthermore, the electrophoresis unit 320 of this embodiment has flow path valves 340 and 370 in the opening passages 346 and 376 to prevent the flow of liquid from above the liquid storage sections 332 and 362. Therefore, according to the electrophoresis unit 320 of this embodiment, when liquid flows from the inflow passages 334 and 364 to the outflow passages 336 and 366, it is possible to prevent liquid from accidentally flowing out from the opening passages 346 and 376.

[0181] Furthermore, in the isobaric step of the electrophoresis method of this embodiment, the liquid is branched from the outflow passages 336 and 366 from the liquid storage sections 332 and 362, and flows out through the opening passages 346 and 376, which function as liquid level adjustment sections 338 and 368, by opening to the outside. Therefore, according to the electrophoresis method of this embodiment, the liquid flowing in the flow paths 330 and 360 can be made to flow out through the outflow passages 336 and 366 or the opening passages 346 and 376.

[0182] Furthermore, in the isobaric step of the electrophoresis method of this embodiment, a valve is used to stop the flow of liquid flowing out from above the liquid storage portions 332 and 362 of the opening passages 346 and 376, thereby stopping the liquid inside the opening passages 346 and 376. Therefore, according to the electrophoresis method of this embodiment, when liquid flows from the inflow passages 334 and 364 to the outflow passages 336 and 366, accidental leakage of liquid from the opening passages 346 and 376 can be prevented.

[0183] Furthermore, in the above description, flow path valves 340 and 370 are provided in the capillary electrophoresis analysis apparatus 310, but are not limited thereto; the electrophoresis unit 320 may also have flow path valves 340 and 370. That is, flow path valves 340 and 370 may also be provided in the open passages 346 and 376.

[0184] Furthermore, in this embodiment, the same structure as in the first to third embodiments can also achieve the same function and effect as in the first to third embodiments.

[0185] Next, refer to Figures 13 to 14D The capillary electrophoresis analysis apparatus 410 according to the fifth embodiment of the present invention will be described. Furthermore, structural reference numerals identical to those in the first embodiment are used in the capillary electrophoresis analysis apparatus 410 according to the fifth embodiment, and detailed descriptions are omitted.

[0186] [Fifth Implementation Method]

[0187] (structure)

[0188] In this embodiment, the same as in the fourth embodiment, such as... Figures 14A to 14D As shown, inflow passages 434 and 464 are tubular liquid passages extending vertically. Furthermore, flow path valves 440 and 470 are provided upstream of inflow passages 434 and 464 in the capillary electrophoresis analysis apparatus 410. Electrophoresis solution RS or sample solution SS supplied from supply units 44 and 74 flows into inflow passages 434 and 464 through flow path valves 440 and 470. In other words, in this embodiment, flow path valves 440 and 470 are provided upstream of inflow passages 434 and 464 and at the opening ends of opening passages 446 and 476, respectively. Additionally, capillary tubes 424 connect liquid storage units 432 and 462 to each other.

[0189] Furthermore, in this embodiment, the outflow passages 436 and 466 are externally connected to a pressure pump via a switching valve such as a three-way valve (not shown), enabling the flow from the downstream side of the outflow passages 436 and 466 ( Figures 14A to 14DCompressed air is supplied from the upper side of the outlet passages 430 and 466. In other words, in this embodiment, the flow paths 430 and 460 are pressurized by supplying compressed air from the downstream side of the outlet passages 436 and 466.

[0190] Furthermore, in this embodiment, the viscosity of the electrophoresis solution RS and the sample solution SS is greater than that of the electrophoresis solution RS and the sample solution SS in the fourth embodiment. Therefore, in this embodiment, if the flow valves 440 and 470 of the opening passages 446 and 476 are opened only as in step S408 of the fourth embodiment, the liquid level LH ​​is difficult to drop.

[0191] Furthermore, the other structures are the same as in the fourth embodiment. That is, in this embodiment, the first opening passage 446 is an example of a "first liquid level adjustment unit", and the second opening passage 476 is an example of a "second liquid level adjustment unit".

[0192] Next, refer to Figures 13 to 14D The steps of the electrophoresis method in this embodiment will be explained. Furthermore, in Figures 14A to 14D The diagram shows the vertical positional relationship of each structure in flow paths 430, 460, flow path valves 440, 470, and capillary tube 424, sometimes omitting other structures.

[0193] (Electrophoresis method)

[0194] In this embodiment, the control unit 22 executes a program (not shown) sequentially. Figure 13 The steps are shown.

[0195] First, in step S502, as Figure 14A As shown, the control unit 22 drives the first supply unit 44 to introduce the electrophoretic solution RS into the first flow path 430, filling the first inflow passage 434, the first storage unit 432, and the first outflow passage 436 with the electrophoretic solution RS. Furthermore, in this embodiment, as long as the liquid level of the electrophoretic solution RS is located vertically above the first opening passage 446, the liquid level can also be located in the middle of the first outflow passage 436. In other words, the first outflow passage 436 may not be completely filled with the electrophoretic solution RS.

[0196] Next, in step S504, the control unit 22 fills the capillary 424 from the first flow path 430 using the electrophoretic liquid RS. In this embodiment, the electrophoretic liquid RS is pressurized by compressed air supplied from the downstream side of the first outflow passage 436, thereby filling the capillary 424.

[0197] Next, in step S506, the control unit 22, as follows: Figure 14BAs shown, the second supply unit 74 is driven to introduce the sample solution SS into the second flow path 460, filling the second inflow passage 464, the second storage unit 462, and the second outflow passage 466 with the sample solution SS. Furthermore, in this embodiment, the liquid level of the sample solution SS only needs to be located vertically above the second opening passage 476; the liquid level can also be located midway through the second outflow passage 466. In other words, the second outflow passage 466 may not be completely filled with the sample solution SS.

[0198] Next, in step S508, as Figure 14C As shown, control unit 22 stops driving supply units 44 and 74, and opens flow valves 440 and 470 in opening passages 446 and 476. Additionally, in step S508, control unit 22 closes flow valves 440 and 470 in inflow passages 434 and 464.

[0199] Next, in step S510, as Figure 14D As shown, the control unit 22 supplies compressed air from the downstream side of the outflow passages 436 and 466 to pressurize the outflow passages 436 and 466. Thus, as... Figure 14D As shown, the electrophoresis solution RS and the sample solution SS are discharged from the open passages 446 and 476, thereby causing the liquid levels LH of the electrophoresis solution RS and the sample solution SS to decrease.

[0200] Next, in step S512, the control unit 22 applies a voltage to a pair of electrodes 28 disposed in the second flow path 460 and the first flow path 430 to start electrophoresis. In addition, the control unit 22 determines the concentration and type of components contained in the sample solution SS by using the detector 26 to measure the composition of the fluid moving in the capillary 424 due to electroosmosis.

[0201] In this embodiment, the liquid levels LH at both ends of the capillary 424 are adjusted through the above steps. Specifically, step S502 in this embodiment is an example of "allowing liquid to flow in from the first inflow passage," step S506 is an example of "allowing liquid to flow in from the second inflow passage," and step S504 is an example of "filling the capillary with liquid." Furthermore, steps S508 and S510 in this embodiment are examples of "ordering the vertical positions of the liquid surfaces in the first and second liquid reservoirs to be equal, i.e., isobaric order," and step S512 is an example of "ordering the components contained in the liquid inside the capillary to undergo electrophoresis."

[0202] The component analysis apparatus and electrophoresis method of this embodiment can achieve the following effects and benefits.

[0203] (Functions and Effects)

[0204] In this embodiment, the electrophoresis unit 420 has flow path valves 440 and 470 in the inflow passages 434 and 464 to prevent the flow of liquid flowing from above into the liquid storage sections 432 and 462.

[0205] In the electrophoresis unit 420 of this embodiment, the inflow passages 434 and 464 are equipped with flow path valves 440 and 470. The flow path valves 440 and 470 stop the liquid flowing into the passages 434 and 464, thereby not affecting the pressure of the liquid in the reservoirs 432 and 462. In other words, the pressure of the liquid applied to the reservoirs 432 and 462 depends on the external pressure and the liquid height in the outflow passages 436 and 466. Therefore, the pressure of the liquid applied to the first reservoir 432 is equal to the pressure of the liquid applied to the second reservoir 462. Thus, according to the electrophoresis unit 420 of this embodiment, without discharging the liquid inside the flow paths 430 and 460 in the reservoirs 432 and 462 from the inflow passages 434 and 464, the pressure of the liquid applied to both ends of the capillary 424 can be made consistent.

[0206] In addition, in the isobaric step, the electrophoresis method of this embodiment uses flow path valves 440 and 470 to stop the flow of liquid into the liquid storage sections 432 and 462 of the inflow passages 434 and 464 from above, thereby stopping the liquid flowing into the interior of the inflow passages 434 and 464.

[0207] The electrophoresis method of this embodiment stops the liquid flowing into passages 434 and 464 by using flow path valves 440 and 470, thereby not affecting the pressure of the liquid in the reservoirs 432 and 462. In other words, the pressure of the liquid applied to the reservoirs 432 and 462 depends on the external pressure and the liquid height in the outflow passages 436 and 466, so the pressure of the liquid applied to the first reservoir 432 is equal to the pressure of the liquid applied to the second reservoir 462. Thus, according to the electrophoresis method of this embodiment, without discharging the liquid inside the flow paths 430 and 460 in the reservoirs 432 and 462 from the inflow passages 434 and 464, the pressure of the liquid applied to both ends of the capillary 424 can be made consistent.

[0208] Furthermore, in the above description, flow path valves 440 and 470 are provided in the capillary electrophoresis analysis apparatus 410, but are not limited thereto; the electrophoresis unit 420 may also have flow path valves 440 and 470. That is, flow path valves 440 and 470 may also be provided in the inflow passages 434 and 464 and the opening passages 446 and 476.

[0209] Additionally, in the above description, in step S504, the electrophoretic solution RS is pressurized by compressed air supplied from the downstream side of the first outflow passage 436. However, alternatively, the capillary 424 can be filled by evacuating the second flow path 460. Furthermore, in this case, a pressure reducing pump can be connected to the external side of the second outflow passage 466 or the second opening passage 476.

[0210] Furthermore, in the above description, in step S510, the outflow passages 436 and 466 are pressurized by supplying compressed air from the downstream side. However, this is not limited to this; a vacuum can also be applied to the outer side of the opening passages 446 and 476. In other words, instead of pressurizing the outflow passages 436 and 466, the electrophoresis solution RS or the sample solution SS can be discharged by depressurizing the opening passages 446 and 476, thus becoming... Figure 14D In this case, a pressure reducing pump can be connected to the outside of the opening passages 446 and 476.

[0211] Furthermore, in this embodiment, the same structure as in the first to fourth embodiments can also achieve the same function and effect as in the first to fourth embodiments.

[0212] Next, refer to Figures 15 to 17D The capillary electrophoresis analysis apparatus 510 according to the sixth embodiment of the present invention will be described. Furthermore, the same structural reference numerals as those in the first embodiment are used in the capillary electrophoresis analysis apparatus 510 according to the sixth embodiment, and specific descriptions are omitted.

[0213] [Sixth Implementation Method]

[0214] (structure)

[0215] In this embodiment, the same as in the fifth embodiment, such as... Figure 15 As shown, inflow passages 534 and 564 are tubular liquid passages extending vertically. Furthermore, upstream of inflow passages 534 and 564 in the capillary electrophoresis analysis apparatus 510, flow path valves 540 and 570 are provided at the open ends of flow paths 530 and 560. Electrophoresis solution RS or sample solution SS supplied from supply units 44 and 74 flows into inflow passages 534 and 564 through flow path valves 540 and 570. Additionally, capillary tube 524 connects liquid storage units 532 and 562 to each other.

[0216] Furthermore, in this embodiment, such as Figure 15As shown, the inflow passages 534 and 564 extend vertically and branch horizontally. The portions branching from the inflow passages 534 and 564 and extending horizontally are the opening passages 546 and 576. That is, the electrophoresis unit 520 of this embodiment has two first opening passages 546 and two second opening passages 576, respectively.

[0217] Furthermore, the vertical positions H of each of the first opening passages 546 and the second opening passages 576 are equal. That is, both first opening passages 546 are examples of the "first opening passage" in this embodiment, and both second opening passages 576 are examples of the "second opening passage" in this embodiment. In addition, the opening passages 546 and 576 in this embodiment have flow path valves 540 and 570 respectively on the side opposite to the inflow passages 534 and 564, and open to the outside of the flow paths 530 and 560, with the pressure at the opening end being equal to the external air pressure outside the flow paths 530 and 560. In other words, when the flow path valves 540 and 570 of the opening passages 546 and 576 are open, the internal pressure of the opening passages 546 and 576 is equal to the external air pressure.

[0218] Furthermore, in this embodiment, the inflow passages 534 and 564 are externally connected to a pressure pump via a switching valve such as a three-way valve (not shown), enabling the pump to operate from the upstream side of the inflow passages 534 and 564. Figures 17A to 17D Compressed air is supplied from the upper side of the inflow passages 534 and 564. In other words, the flow paths 530 and 560 in this embodiment are pressurized by supplying compressed air from the upstream side of the inflow passages 534 and 564.

[0219] Furthermore, the other structures are the same as in the fifth embodiment. That is, in this embodiment, the first opening passage 546 is an example of a "first liquid level adjustment unit", and the second opening passage 576 is an example of a "second liquid level adjustment unit". In addition, in this embodiment, flow path valves 540 and 570 are respectively provided in the inflow passages 534 and 564 and the opening passages 546 and 576.

[0220] Next, refer to Figures 16 to 17D The steps of the electrophoresis method in this embodiment will be explained. Furthermore, in Figures 17A to 17D The diagram shows the vertical positional relationship of each structure in flow paths 530, 560, flow path valves 540, 570, and capillary tube 524, sometimes omitting other structures.

[0221] (Electrophoresis method)

[0222] In this embodiment, the control unit 22 executes a program (not shown) sequentially. Figure 16 The steps are shown.

[0223] First, in step S602, as Figure 17A As shown, the control unit 22 drives the first supply unit 44 to introduce the electrophoresis liquid RS into the first flow path 530, and fills the first inflow passage 534, the first storage unit 532 and the first outflow passage 536 with the electrophoresis liquid RS.

[0224] Next, in step S604, the control unit 22 fills the capillary 524 from the first flow path 530 using the electrophoretic liquid RS. Alternatively, in this embodiment, the electrophoretic liquid RS is pressurized by compressed air supplied from the downstream side of the first outflow passage 536, thereby filling the capillary 524.

[0225] Next, in step S606, the control unit 22, as follows: Figure 17B As shown, the second supply unit 74 is driven to introduce the sample solution SS into the second flow path 560, and the sample solution SS fills the second inflow passage 564, the second storage unit 562 and the second outflow passage 566.

[0226] Next, in step S608, as Figure 17C As shown, control unit 22 stops driving supply units 44 and 74, and opens flow path valves 540 and 570 of opening passages 546 and 576 on the outflow passages 536 and 566. In addition, control unit 22 closes flow path valves 540 and 570 of inflow passages 534 and 564 in step S608.

[0227] Next, in step S610, as Figure 17D As shown, the control unit 22 supplies compressed air from the downstream side of the outflow passages 536 and 566 to pressurize the outflow passages 536 and 566. Thus, as... Figure 17D As shown, the electrophoresis solution RS and the sample solution SS are discharged from the openings 546 and 576 of the outflow passages 536 and 566, thereby causing the liquid levels LH of the electrophoresis solution RS and the sample solution SS to decrease.

[0228] Furthermore, in step S610, the control unit 22 of this embodiment further opens the flow valves 540 and 570 of the inflow passages 534 and 564 and the flow valves 540 and 570 of the opening passages 546 and 576 on the side of the inflow passages 534 and 564.

[0229] Furthermore, in step S610, the control unit 22 of this embodiment further includes, as follows: Figure 17D As shown, compressed air is supplied from the upstream side of inflow passages 534 and 564 to pressurize them. Thus, as... Figure 17D As shown, the electrophoresis solution RS and the sample solution SS are discharged from the opening passages 546 and 576 of the inflow passages 534 and 564, thereby causing the liquid levels LH of the electrophoresis solution RS and the sample solution SS to decrease.

[0230] Next, in step S612, the control unit 22 applies a voltage to a pair of electrodes 28 disposed in the first flow path 530 and the second flow path 560 to start electrophoresis. In addition, the control unit 22 determines the concentration and type of components contained in the sample solution SS by using the detector 26 to measure the composition of the fluid moving in the capillary 524 due to electroosmosis.

[0231] In this embodiment, the liquid levels LH at both ends of the capillary 524 are adjusted through the above steps. Specifically, step S602 in this embodiment is an example of "allowing liquid to flow in from the first inflow passage," step S606 is an example of "allowing liquid to flow in from the second inflow passage," and step S604 is an example of "filling the capillary with liquid." Furthermore, steps S608 and S610 in this embodiment are examples of "ordering the vertical positions of the liquid surfaces in the first and second liquid reservoirs to be equal, i.e., isobaric order," and step S612 is an example of "ordering the components contained in the liquid inside the capillary to undergo electrophoresis."

[0232] The component analysis apparatus and electrophoresis method of this embodiment can achieve the following effects and benefits.

[0233] (Functions and Effects)

[0234] The electrophoresis unit 520 of this embodiment has opening passages 546 and 576 that open to the outside in the inflow passages 534 and 564 and the outflow passages 536 and 566, respectively. Therefore, compared with the case where the opening passages 546 and 576 are only formed in the outflow passages 536 and 566 or the inflow passages 534 and 564, the electrophoresis unit 520 of this embodiment can accelerate the unification of the liquid levels LH of the electrophoresis solution RS or the sample solution SS.

[0235] Furthermore, according to the electrophoresis unit 520 of this embodiment, even if air is mixed into the electrophoresis solution RS or sample solution SS flowing into the inflow passages 534 and 564, the air can be easily discharged from either opening passage 546 or 576 during the isobaric step. Therefore, the electrophoresis unit 520 of this embodiment can maintain a consistent liquid level LH ​​regardless of the sealing performance of the upstream side of the inflow passages 534 and 564.

[0236] Furthermore, in this embodiment, the electrophoresis method discharges the electrophoretic solution RS or the sample solution SS from the externally opening passages 546 and 576, which are respectively provided in the inflow passages 534 and 564 and the outflow passages 536 and 566. Therefore, according to this embodiment, compared to the case where the electrophoretic solution RS or the sample solution SS is discharged only from the opening passages 546 and 576 provided in the inflow passages 534 and 564, the electrophoresis method can accelerate the equalization of the liquid level LH ​​of the electrophoretic solution RS or the sample solution SS.

[0237] Furthermore, in the electrophoresis method of this embodiment, during the isobaric step, the liquid flows out from the inflow passages 534 and 564. Therefore, even if air is mixed into the electrophoresis solution RS or sample solution SS flowing into the inflow passages 534 and 564, the air can be easily discharged from either opening passage 546 or 576. Thus, according to the electrophoresis method of this embodiment, the liquid level LH ​​can be kept consistent regardless of the sealing performance of the upstream side of the inflow passages 534 and 564.

[0238] Furthermore, in the above description, flow path valves 540 and 570 are provided in the capillary electrophoresis analysis apparatus 510, but are not limited thereto; the electrophoresis unit 520 may also have flow path valves 540 and 570. That is, flow path valves 540 and 570 may also be provided in the inflow passages 534 and 564 and the multiple opening passages 546 and 576, respectively.

[0239] Furthermore, in this embodiment, the same structure as in the first to fifth embodiments can also achieve the same function and effect as in the first to fifth embodiments.

[0240] [Other Implementation Methods]

[0241] It should be noted that in the above description, the capillary 24 connects the side surfaces of the first liquid reservoir 32 and the second liquid reservoir 62 in a straight line, but the technology of the present invention is not limited to this. For example, if the detector 26 can determine the components in the capillary 24, the capillary 24 can also be connected to the first liquid reservoir 32 and the second liquid reservoir 62 in a curved manner. That is, the shape of the capillary 24 is not limited to the shape described above.

[0242] Furthermore, in the above description, the bottom surface of the liquid storage section 62 is positioned where the vertical position H in the flow paths 30 and 60 is lowest, but the technology of the present invention is not limited to this. If the vertical positions H of the first liquid level adjustment section 38 and the second liquid level adjustment section 68 are equal to each other, then the liquid levels LH of the first liquid storage section 32 and the second liquid storage section 62 are equal. Therefore, for example, the inflow passage 34, the inflow passage 64, and the outflow passage 36, the outflow passage 66 can also be connected to the bottom surface of the liquid storage section 62. In other words, the fluid flowing into the liquid storage section 62 and the fluid flowing out of the liquid storage section 62 can also pass through positions where the vertical position H is lower than the bottom surface of the liquid storage section 62.

[0243] Furthermore, in the above description, the shapes of the first flow path 30 and the second flow path 60 are the same in any embodiment, but the technology of the present invention is not limited thereto. That is, as long as the vertical positions H of the first liquid level adjustment part 38 and the second liquid level adjustment part 68 are equal, the shapes of the first flow path 30 and the second flow path 60 can also adopt the structure of any of the above embodiments. In other words, the shapes of the first flow path 30 and the second flow path 60 can also adopt shapes of different embodiments.

[0244] Furthermore, in the above description, in any embodiment, the electrophoresis unit 20 integrally forms the first flow path 30, the second flow path 60, and the capillary 24, but the technology of the present invention is not limited thereto. For example, the electrophoresis unit 20 may also be formed by combining multiple components that are divided in the vertical or horizontal direction.

[0245] Furthermore, in the above description, in the second embodiment, the electrophoresis solution RS and the sample solution SS flow into the flow paths 130 and 160 from the inflow passages 134 and 164, respectively; however, the technology of the present invention is not limited to this. That is, in the second embodiment, the sample solution SS and the electrophoresis solution RS may also flow into the flow paths 130 and 160 from the outflow passages 136 and 166, respectively. Additionally, regarding this variation, the third to sixth embodiments may also be used in the same way.

[0246] In these variations, the same effects and functions as described above can also be achieved.

[0247] The embodiments of the present invention have been described above with reference to the accompanying drawings. However, it is obvious that anyone with ordinary knowledge in the technical field to which the present invention pertains can conceive of various modifications or applications within the scope of the technical concept described in the claims, and these are of course also understood to fall within the technical scope of the present invention.

[0248] Furthermore, preferred embodiments of the present invention are shown below.

[0249] (Postscript 1)

[0250] An electrophoresis unit, comprising:

[0251] The first flow path has a first liquid storage section, a first inflow passage through which liquid flows into the first liquid storage section, a first outflow passage through which liquid flows out of the first liquid storage section, and a first liquid level adjustment section that opens to the outside.

[0252] The second flow path includes: a second liquid storage section, a second inflow passage through which liquid flows into the second liquid storage section, a second outflow passage through which liquid flows out of the second liquid storage section, and a second liquid level adjustment section that opens to the outside;

[0253] A capillary tube, connecting the first liquid reservoir to the second liquid reservoir; and

[0254] Electrodes are respectively disposed inside the first flow path and inside the second flow path;

[0255] The vertical positions of the first liquid level adjustment part and the second liquid level adjustment part are equal to each other.

[0256] (Postscript 2)

[0257] As described in Appendix 1, the electrophoresis unit wherein the first outflow passage is the first liquid level adjustment section facing laterally within the vertical direction of the first liquid storage section.

[0258] (Note 3)

[0259] The electrophoresis unit as described in Appendix 2 includes a valve in the first inflow passage that prevents the flow of liquid into the first reservoir from above.

[0260] (Note 4)

[0261] As described in Appendix 1, the electrophoresis unit wherein the first inflow passage is the first liquid level adjustment section that allows liquid to flow from above into the first liquid storage section and opens to the outside.

[0262] (Note 5)

[0263] As described in Appendix 1, the electrophoresis unit wherein the first opening passage branching from the first outflow passage is the first liquid level adjustment section opening to the outside.

[0264] (Note 6)

[0265] The electrophoresis unit as described in Appendix 5 includes a valve in the first opening passage that prevents the flow of liquid from above the first liquid reservoir.

[0266] (Note 7)

[0267] According to the electrophoresis unit described in Appendix 6, a valve is provided in the first inflow passage to prevent the flow of liquid flowing into the first reservoir from above.

[0268] (Postscript 8)

[0269] A component analysis device, comprising:

[0270] Electrophoresis unit as described in any one of Appendix 1 to Appendix 7;

[0271] The supply unit supplies the sample solution to the electrophoresis unit; and

[0272] The control unit controls the supply unit.

[0273] (Note 9)

[0274] The component analysis apparatus according to Appendix 8 further includes: a detector for detecting the composition of the liquid flowing inside the capillary.

[0275] (Postscript 10)

[0276] An electrophoresis method, comprising:

[0277] The step of allowing liquid to flow from a first inflow passage into a first flow path, wherein the first flow path has a first liquid level adjustment section that opens to the outside and makes the pressure of the first liquid storage section equal to the external pressure;

[0278] The step of allowing liquid to flow from a second inflow passage into a second flow path, wherein the second flow path has a second liquid level adjustment that opens to the outside so that the pressure of the second liquid storage section is equal to the external pressure and the vertical position is equal to the vertical position of the first liquid level adjustment section.

[0279] The step of filling the capillary tube that connects the first liquid reservoir to the second liquid reservoir with liquid;

[0280] In the isobaric step, the first liquid level adjustment unit and the second liquid level adjustment unit are used to make the vertical positions of the liquid levels in the liquid accumulated in the first liquid storage unit and the second liquid storage unit equal; and

[0281] The step of electrophoresis of components contained in the liquid inside the capillary by means of electrodes respectively disposed inside the first flow path and the second flow path.

[0282] (Postscript 11)

[0283] The electrophoresis method described in Appendix 10, wherein,

[0284] In the isobaric step, liquid is allowed to flow out from the first outflow passage, and the vertical position of the liquid level in the liquid accumulated in the first liquid storage section is adjusted. The first outflow passage is the first liquid level adjustment section facing to the side within the vertical direction of the first liquid storage section.

[0285] (Postscript 12)

[0286] The electrophoresis method described in Appendix 11, wherein,

[0287] In the isobaric step, a valve is used to prevent the flow of liquid into the first reservoir portion of the first inflow passage from above, thereby preventing the flow of liquid inside the first inflow passage.

[0288] (Postscript 13)

[0289] The electrophoresis method described in Appendix 10, wherein,

[0290] In the isobaric step, liquid is allowed to flow into the first liquid storage section from above, and liquid is allowed to flow out from the first liquid level adjustment section, i.e., the first inflow passage, which opens to the outside.

[0291] (Postscript 14)

[0292] The electrophoresis method described in Appendix 10, wherein,

[0293] In the isobaric step, liquid is allowed to flow out from the first outflow passage, which allows liquid to flow out from the first liquid storage section and from the first liquid level adjustment section, i.e., the first opening passage, which opens to the outside.

[0294] (Postscript 15)

[0295] The electrophoresis method described in Appendix 14, wherein,

[0296] In the isobaric step, a valve is used to stop the flow of liquid flowing out from above the first liquid reservoir in the first open passage, thereby stopping the liquid inside the first open passage.

[0297] (Postscript 16)

[0298] The electrophoresis method described in Appendix 15, wherein,

[0299] In the isobaric step, a valve is used to prevent the flow of liquid into the first reservoir portion of the first inflow passage from above, thereby blocking the liquid inside the first inflow passage.

Claims

1. An electrophoresis unit, comprising: a first flow path having a first liquid storage portion, a first inflow passage through which liquid flows into the first liquid storage portion, a first outflow passage through which liquid flows out of the first liquid storage portion, and a first liquid level adjustment portion open to the outside; a second flow path having: a second liquid storage portion, a second inflow passage through which liquid flows into the second liquid storage portion, a second outflow passage through which liquid flows out of the second liquid storage portion, and a second liquid level adjustment portion open to the outside; a capillary tube connected from the first liquid storage part to the second liquid storage part; as well as Electrodes are respectively arranged inside the first flow path and inside the second flow path; The first liquid level adjusting portion and the second liquid level adjusting portion are located at the same position in the vertical direction.

2. The electrophoresis unit according to claim 1, wherein: The first outflow passage is the first liquid level adjusting portion that is directed to the side within the vertical direction of the first liquid storage portion.

3. The electrophoresis unit according to claim 2, wherein: The first inflow passage is provided with a valve that blocks the flow of liquid flowing into the first liquid storage portion from above.

4. The electrophoresis unit according to claim 1, wherein: The first inflow passage is the first liquid level adjusting portion that allows liquid to flow into the first liquid storage portion from above and is open to the outside.

5. The electrophoresis unit according to claim 1, wherein: The first open passage branched from the first outflow passage is the first liquid level adjusting portion opened to the outside.

6. The electrophoresis unit according to claim 5, wherein: The first open passage is provided with a valve that blocks the flow of liquid flowing out from above the first liquid storage section.

7. The electrophoresis unit according to claim 6, wherein: The first inflow passage is provided with a valve that blocks the flow of liquid flowing into the first liquid storage portion from above.

8. A component analysis device, comprising: The electrophoresis unit according to any one of claims 1 to 7; a supply unit for supplying a sample solution to the electrophoresis unit; as well as The control unit controls the supply unit.

9. The component analysis device according to claim 8, further comprising: The detector detects components of the liquid flowing inside the capillary.

10. An electrophoresis method comprising: A step of causing liquid to flow from the first inflow passage into a first liquid storage portion of a first flow path, the first flow path having a first liquid level adjustment portion that opens to the outside and makes the pressure of the first liquid storage portion equal to the external pressure; A step of causing liquid to flow from the second inflow passage into a second liquid storage portion of a second flow path, the second flow path having a second liquid level adjusting portion that is open to the outside so that the pressure of the second liquid storage portion is equal to the external pressure and the vertical position is equal to the vertical position of the first liquid level adjusting portion; A step of filling a capillary connected from the first liquid storage part to the second liquid storage part with a liquid; an equal pressure step of making the positions of the liquid levels in the first liquid storage part and the second liquid storage part in the vertical direction equal by using the first liquid level adjusting part and the second liquid level adjusting part; as well as A step of electrophoresing components contained in the liquid inside the capillary using electrodes respectively provided inside the first flow channel and the second flow channel.

11. The electrophoresis method according to claim 10, wherein: In the isobaric step, the liquid is caused to flow out from a first outflow passage to adjust the vertical position of the liquid surface in the liquid accumulated in the first liquid storage section. The first outflow passage is the first liquid level adjustment section facing the side within the vertical direction of the first liquid storage section.

12. The electrophoresis method according to claim 11, wherein: In the pressure equalization step, the flow of the liquid in the first inflow passage is blocked by using a valve that blocks the flow of the liquid flowing into the first liquid storage portion provided in the first inflow passage from above.

13. The electrophoresis method according to claim 10, wherein: In the pressure-equalizing step, the liquid is caused to flow into the first liquid storage portion from above, and the liquid is caused to flow out from the first liquid level adjusting portion, ie, the first inflow passage, which is open to the outside.

14. The electrophoresis method according to claim 10, wherein: In the pressure equalization step, the liquid is made to flow out from a first outflow passage through which the liquid flows out from the first liquid storage portion and flows out from a first opening passage that is the first liquid level adjusting portion opened to the outside.

15. The electrophoresis method according to claim 14, wherein: In the pressure equalization step, the liquid in the first open passage is stopped using a valve that stops the flow of the liquid flowing out from above the first liquid storage portion included in the first open passage.

16. The electrophoresis method according to claim 15, wherein: In the pressure equalization step, the liquid in the first inflow passage is blocked by using a valve that blocks the flow of the liquid flowing into the first liquid storage portion included in the first inflow passage from above.

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