Anti-fatigue high-efficiency low-sugar sports drink based on biotechnology and preparation method thereof

Abstract

The present application relates to the technical field of anti-fatigue beverage, in particular to an anti-fatigue high-efficiency sugar-reduced sports beverage based on biotechnology and a preparation method thereof, which comprises the following components: water 80-100 parts by weight, etherified rhodiola glycoside 5-10 parts by weight, hawthorn extract 8-15 parts by weight, green tea extract 6-12 parts by weight and the like. The present application adds methyl glucoside to undergo etherification reaction with rhodiola glycoside, and the etherification product generated is more stable when the environment changes, and the active ingredients are not easy to decompose; at the same time, the improvement of water solubility of methyl glucoside promotes the absorption of rhodiola glycoside, indirectly enhances the anti-fatigue effect, and thus relieves fatigue more quickly.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of anti-fatigue beverages, in particular to an anti-fatigue high-efficiency low-sugar sports beverage based on biotechnology and a preparation method thereof. BACKGROUND

[0002] Most of the sports beverages on the market are mainly composed of artificial synthetic ingredients and high-sugar formulations; although traditional high-sugar sports beverages can quickly replenish energy, they pose a risk of blood sugar fluctuation for diabetic patients, people concerned about health, and consumers pursuing a low-sugar diet, and long-term consumption may cause health problems such as obesity and cardiovascular disease, thereby limiting the consumer group; in addition, some artificial synthetic ingredients may have potential health risks, and consumers' demand for more natural and healthy sports beverages is increasing.

[0003] Plant extracts are rich in various bioactive components such as flavonoids, polysaccharides, saponins, etc., and have various effects such as antioxidant, anti-fatigue, and immune regulation; however, the active components in plant extracts have complex chemical structures, and during processing and storage, their effective components are easily degraded by factors such as light, temperature, and oxygen, thereby reducing the efficacy; in view of this, we propose an anti-fatigue high-efficiency low-sugar sports beverage based on biotechnology and a preparation method thereof. SUMMARY

[0004] The present application aims to provide an anti-fatigue high-efficiency low-sugar sports beverage based on biotechnology and a preparation method thereof, to solve the problem of complex chemical structure of active components in plant extracts, which are easily degraded by factors such as light, temperature, and oxygen during processing and storage, thereby reducing the efficacy.

[0005] To achieve the above-mentioned purpose, the present application provides an anti-fatigue high-efficiency low-sugar sports beverage based on biotechnology, comprising the following components: water 80-100 parts by weight, etherified rhodioloside 5-10 parts by weight, hawthorn extract 8-15 parts by weight, green tea extract 6-12 parts by weight, guarana extract 3-8 parts by weight, wolfberry extract 8-15 parts by weight, erythritol 20-30 parts by weight, sodium chloride 1-3 parts by weight, potassium chloride 0.5-2 parts by weight, sour agent 2-5 parts by weight, gamma-aminobutyric acid 1-3 parts by weight, L-ornithine 1-3 parts by weight, coenzyme Q10 0.5-2 parts by weight, choline 0.5-2 parts by weight, sodium carboxymethyl cellulose 0.5-2 parts by weight.

[0006] The etherified rhodioloside is prepared by etherification reaction of rhodioloside with methyl glucoside and addition of polyethylene glycol-phosphorylcholine.

[0007] As a preferred embodiment, the preparation method of the etherified rhodioloside is as follows:

[0008] S1.1, respectively, take the following weight parts of components: 10-20 parts by weight of rhodioloside, 15-30 parts by weight of methyl glucoside, 3-8 parts by weight of polyethylene glycol-phosphoryl choline, 5-12 parts by weight of potassium carbonate;

[0009] S1.2, under nitrogen protection, dissolve rhodioloside and methyl glucoside in deionized water, stir at a speed of 100-200 rpm for 30-60 min, add potassium carbonate solution to adjust the pH value of the solution; slowly add polyethylene glycol-phosphoryl choline, and place the reaction system in a constant temperature water bath at 40-60℃, and stir at a speed of 300-400 rpm for 10-24 h;

[0010] Under the conditions of nitrogen protection and potassium carbonate alkalinity, the 7-hydroxyl group of rhodioloside is first deprotonated to form a nucleophilic alkoxy negative ion; the negative ion attacks the 2-hydroxyl carbon (β-D configuration) of methyl glucoside, initiating a nucleophilic substitution reaction; the 2-hydroxyl group of methyl glucoside is activated under the action of alkali, and its leaving group (-OH) combines with the alkoxy negative ion to form an ether bond.

[0011] Some groups in the rhodioloside molecule (such as hydroxyl groups) have certain reactivity and are easily reacted with external substances or changed themselves under certain conditions; by etherification with methyl glucoside, these active groups are converted into relatively stable ether bond structures, which have high bond energy and are not easily broken under general environmental conditions, thereby improving the stability of the entire molecule; the solubility of rhodioloside itself may be limited, while methyl glucoside has multiple hydroxyl groups and is a substance with strong hydrophilicity; after etherification, the product molecule introduces the structural fragments of methyl glucoside, increasing the interaction sites between the molecule and water molecules, allowing more hydrogen bonds and other forces to form between the molecule and water molecules, thereby improving the solubility of the product in water; etherification changes the molecular structure of rhodioloside, on the one hand, making the original rhodioloside bind more tightly and specifically to the target, thereby enhancing the original biological activity; on the other hand, the newly introduced methyl glucoside structure will endow the product with the ability to interact with other targets, thereby producing new biological activity; after etherification, some groups in rhodioloside are modified, and the overall properties of the molecule change, making it less likely to interact with sensitive components in biological tissues when it comes into contact with the skin, mucous membranes, etc., reducing the possibility of triggering a stimulation reaction.

[0012] Polyethylene glycol-phosphorylcholine has a unique molecular structure and properties, its polyethylene glycol segment has good solubility and flexibility, can significantly increase the flowability and collision probability of reactant molecules in the reaction system, thereby promoting the approach and reaction of rhodioside and methyl glucoside, increasing the rate of etherification reaction, shortening the reaction time, and improving the production efficiency; during the etherification reaction, the generated reaction intermediates usually have high activity and instability, polyethylene glycol-phosphorylcholine forms specific interactions (such as hydrogen bonds and electrostatic interactions) with these intermediates, effectively stabilizing the intermediates, making them more smoothly participate in subsequent reactions, reducing the possibility of intermediate decomposition or side reactions, thereby improving the selectivity of the reaction and the yield of the product; in addition, polyethylene glycol-phosphorylcholine can form a protective film-like structure around the product molecules, reducing the influence of external environmental factors (such as oxygen, moisture, light, etc.) on the product, reducing the possibility of product oxidation, hydrolysis, degradation, etc. reaction, thereby improving the chemical stability and storage stability of the product.

[0013] S1.3, after the reaction, the reaction liquid is filtered to remove insoluble impurities; the filtrate is subjected to rotary evaporation at 50-60℃ at a speed of 100-150rpm, and then dried in a vacuum drying box to obtain etherified rhodioside.

[0014] Preferably, in S1.2, the concentration of the potassium carbonate solution is 5%-10%.

[0015] Preferably, in S1.2, the pH value of the potassium carbonate adjusting solution is 9-10.

[0016] Preferably, in S1.2, the speed of slowly adding polyethylene glycol-phosphorylcholine is 1-2 parts by weight per minute.

[0017] Preferably, in S1.3, the temperature of vacuum drying is 40-50℃, and the drying time is 4-8h.

[0018] In another aspect, the present application provides a preparation method of a bio-technology-based anti-fatigue high-efficiency low-sugar sports drink, which is used for the bio-technology-based anti-fatigue high-efficiency low-sugar sports drink of any one of the above, comprising the following steps:

[0019] S2.1, respectively, take the following weight parts of components: water 80-100 parts by weight, etherified rhodiol 5-10 parts by weight, hawthorn extract 8-15 parts by weight, green tea extract 6-12 parts by weight, guarana extract 3-8 parts by weight, medlar extract 8-15 parts by weight, erythritol 20-30 parts by weight, sodium chloride 1-3 parts by weight, potassium chloride 0.5-2 parts by weight, sour agent 2-5 parts by weight, gamma-aminobutyric acid 1-3 parts by weight, L-ornithine 1-3 parts by weight, coenzyme Q10 0.5-2 parts by weight, choline 0.5-2 parts by weight, sodium carboxymethyl cellulose 0.5-2 parts by weight;

[0020] S2.2, add 60-70% water in the container, add etherified rhodiol, hawthorn extract, green tea extract, guarana extract, medlar extract in turn, stir at 15-20℃ with the speed of 100-150rpm for 10-15min; add erythritol, sodium chloride and potassium chloride, continue to stir at the speed of 100-150rpm for 5-10min, to get mixed solution;

[0021] Hawthorn extract contains a large amount of organic acids and dietary fiber, which can promote gastrointestinal peristalsis and help digestion, and its flavonoids also have cardiovascular protection effect; caffeine in green tea extract can stimulate central nervous system and reduce exercise fatigue, and tea polyphenols have strong antioxidant capacity; caffeine and natural sugar in guarana extract can provide energy and enhance endurance; polysaccharides in medlar extract can regulate immune function of the body, and various antioxidants can scavenge free radicals and supplement nutrients lost during exercise.

[0022] S2.3, add sour agent to the mixed solution, stir at the speed of 100-200rpm for 5-10min, adjust pH to 3.5-4.5; add gamma-aminobutyric acid, L-ornithine, coenzyme Q10 and choline, stir at the speed of 100-200rpm for 10-15min until evenly distributed;

[0023] S2.4, add sodium carboxymethyl cellulose solution to the mixed solution in S2.3, stir at the speed of 200-300rpm for 15-30min; add 30-40% water, stir at the speed of 300-400rpm for 10-15min; after mixing evenly, filter, then perform homogenization treatment by high-pressure homogenizer, to get anti-fatigue high-efficiency sugar-reduced sports drink based on biotechnology.

[0024] As preferred, in S2.3, the sour agent is any one of citric acid or isocitric acid.

[0025] As preferred, in the S2.4, the sodium carboxymethyl cellulose solution is prepared by dissolving sodium carboxymethyl cellulose in water at 60-70℃ to obtain a sodium carboxymethyl cellulose solution with a concentration of 1%-2%.

[0026] As preferred, in the S2.4, the pressure of the high-pressure homogenizer is 50-60MPa, and the cycle number is 1-3.

[0027] Compared with the prior art, the present application has the following advantages:

[0028] 1. In the anti-fatigue high-efficiency low-sugar sports drink based on biotechnology and the preparation method, methyl glucoside is added, which can undergo etherification reaction with rhodioloside. The etherification product is not easy to decompose under light and temperature changes, which is beneficial to long-term preservation of the beverage. In addition, the energy produced by the hydrolysis of methyl glucoside can supplement the consumption during exercise, and synergize with the anti-fatigue effect of rhodioloside to relieve exercise fatigue faster and improve body function. The product after the reaction can also improve the taste and bring a mild sweetness to the beverage, optimizing the overall flavor.

[0029] 2. In the anti-fatigue high-efficiency low-sugar sports drink based on biotechnology and the preparation method, polyethylene glycol-phosphorylcholine is added, which can form a structure similar to a protective film around the product molecules, reducing the influence of external environmental factors (such as oxygen, moisture, light, etc.) on the product through steric hindrance effect and physical shielding effect, reducing the possibility of oxidation, hydrolysis, degradation, etc. of the product, thereby improving the chemical stability and storage stability of the product. DETAILED DESCRIPTION

[0030] The technical solutions in the embodiments of the present application will be described clearly and completely below in conjunction with the embodiments of the present application. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor fall within the scope of protection of the present application.

[0031] The present application provides an anti-fatigue high-efficiency low-sugar sports drink based on biotechnology, which comprises the following components: water 80-100 parts by weight, etherified rhodioloside 5-10 parts by weight, hawthorn extract 8-15 parts by weight, green tea extract 6-12 parts by weight, guarana extract 3-8 parts by weight, wolfberry extract 8-15 parts by weight, erythritol 20-30 parts by weight, sodium chloride 1-3 parts by weight, potassium chloride 0.5-2 parts by weight, acidifying agent 2-5 parts by weight, gamma-aminobutyric acid 1-3 parts by weight, L-ornithine 1-3 parts by weight, coenzyme Q10 0.5-2 parts by weight, choline 0.5-2 parts by weight, sodium carboxymethyl cellulose 0.5-2 parts by weight.

[0032] The etherified rhodiolin is prepared by etherification reaction of rhodiolin and methyl glucoside, and addition of polyethylene glycol-phosphoryl choline.

[0033] The acidifying agent is any one of citric acid or isocitric acid, preferably isocitric acid.

[0034] The main active ingredients in the hawthorn extract include flavonoids (quercetin, rutin, hyperoside, etc.), organic acids (maslinic acid, ursolic acid, oleanolic acid, etc.), triterpenes (hawthorn terpene diol, hawthorn terpene triol, etc.), and polysaccharides.

[0035] The main active ingredients in the green tea extract include tea polyphenols (catechin), caffeine, and theanine.

[0036] The main active ingredients in the guarana extract include caffeine, tannic acid, ginsenoside, amino acid, and mineral substance.

[0037] The main active ingredients in the wolfberry extract include wolfberry polysaccharide, carotenoids (β-carotene, zeaxanthin, etc.), flavonoids (quercetin, rutin, etc.), and alkaloids (betaine).

[0038] Example 1: A bio-technology-based anti-fatigue high-efficiency low-sugar sports drink and a preparation method thereof, comprising the following steps:

[0039] S2.1, respectively, take the following weight parts of components: water 80 parts by weight, etherified rhodiolin 5 parts by weight, hawthorn extract 8 parts by weight, green tea extract 6 parts by weight, guarana extract 3 parts by weight, wolfberry extract 8 parts by weight, erythritol 20 parts by weight, sodium chloride 1 part by weight, potassium chloride 0.5 parts by weight, isocitric acid 2 parts by weight, γ-aminobutyric acid 1 part by weight, L-ornithine 1 part by weight, coenzyme Q10 0.5 parts by weight, choline 0.5 parts by weight, sodium carboxymethyl cellulose 0.5 parts by weight;

[0040] S2.2, add 70% water in a container, and sequentially add etherified rhodiolin, hawthorn extract, green tea extract, guarana extract, and wolfberry extract, and stir at a speed of 150 rpm at 20°C for 15 min; then add erythritol, sodium chloride, and potassium chloride, and continue to stir at a speed of 150 rpm for 10 min to obtain a mixed solution;

[0041] S2.3, add isocitric acid to the mixed solution, and stir at a speed of 200 rpm for 10 min to adjust the pH to 4.0; then add γ-aminobutyric acid, L-ornithine, coenzyme Q10, and choline, and fully stir at a speed of 200 rpm for 15 min until uniformly distributed;

[0042] S2.4, add a 2% sodium carboxymethyl cellulose solution to the mixed solution in S2.3, stir at a speed of 300 rpm for 30 min; then add 30% water, stir at a speed of 400 rpm for 15 min; after mixing evenly, filter, and then perform homogenization treatment by a high-pressure homogenizer, at a pressure of 60 MPa, for 3 cycles, to obtain the bio-technology-based anti-fatigue high-efficiency low-sugar sports drink.

[0043] The preparation method of the etherified rhodioloside is as follows:

[0044] S1.1, respectively weigh the following components: 15 parts by weight of rhodioloside, 18 parts by weight of methyl glucoside, 3 parts by weight of polyethylene glycol-phosphorylcholine, and 5 parts by weight of potassium carbonate;

[0045] S1.2, under nitrogen protection, dissolve the rhodioloside and the methyl glucoside in deionized water, stir at a speed of 200 rpm for 40 min, and add a 5% potassium carbonate solution to adjust the pH value of the solution to 9; slowly add the polyethylene glycol-phosphorylcholine at a speed of 2 parts by weight per minute, and place the reaction system in a constant-temperature water bath at 60℃, and stir at a speed of 400 rpm for 20 h;

[0046] S1.3, after the reaction is completed, filter the reaction liquid to remove insoluble impurities; perform rotary evaporation of the filtrate at 60℃ at a speed of 150 rpm, and then place it in a vacuum drying box for drying treatment, at a drying temperature of 50℃ for 8 h, to obtain the etherified rhodioloside.

[0047] Example 2: A bio-technology-based anti-fatigue high-efficiency low-sugar sports drink and a preparation method thereof, comprising the following steps:

[0048] S2.1, respectively weigh the following components: 80 parts by weight of water, 5 parts by weight of etherified rhodioloside, 8 parts by weight of hawthorn extract, 6 parts by weight of green tea extract, 3 parts by weight of guarana extract, 8 parts by weight of Chinese wolfberry extract, 20 parts by weight of erythritol, 1 part by weight of sodium chloride, 0.5 part by weight of potassium chloride, 2 parts by weight of iso-citric acid, 1 part by weight of gamma-aminobutyric acid, 1 part by weight of L-ornithine, 0.5 part by weight of coenzyme Q10, 0.5 part by weight of choline, and 0.5 part by weight of sodium carboxymethyl cellulose;

[0049] S2.2, add 70% water in a container, and then add the etherified rhodioloside, the hawthorn extract, the green tea extract, the guarana extract, and the Chinese wolfberry extract, and stir at a speed of 150 rpm at 20℃ for 15 min; then add the erythritol, the sodium chloride, and the potassium chloride, and continue to stir at a speed of 150 rpm for 10 min, to obtain a mixed solution;

[0050] S2.3, add isocitric acid to the mixed solution, stir at a speed of 200 rpm for 10 min, adjust the pH to 4.0; then add gamma-aminobutyric acid, L-ornithine, coenzyme Q10 and choline, and stir at a speed of 200 rpm for 15 min until evenly distributed;

[0051] S2.4, add a 2% sodium carboxymethyl cellulose solution to the mixed solution in S2.3, stir at a speed of 300 rpm for 30 min; then add 30% water, stir at a speed of 400 rpm for 15 min; after mixing evenly, filter, and then perform homogenization treatment by a high-pressure homogenizer, with a pressure of 60 MPa and 3 cycles, to obtain the bio-technology-based anti-fatigue high-efficiency low-sugar sports drink.

[0052] The preparation method of the etherified rhodioloside is as follows:

[0053] S1.1, respectively weigh the following components: 15 parts by weight of rhodioloside, 21 parts by weight of methyl glucoside, 3 parts by weight of polyethylene glycol-phosphorylcholine, and 5 parts by weight of potassium carbonate;

[0054] S1.2, under nitrogen protection, dissolve the rhodioloside and the methyl glucoside in deionized water, stir at a speed of 200 rpm for 40 min, and add a 5% potassium carbonate solution to adjust the pH value of the solution to 9; slowly add the polyethylene glycol-phosphorylcholine at a speed of 2 parts by weight per minute, and place the reaction system in a constant-temperature water bath at 60°C, and stir at a speed of 400 rpm for 20 h;

[0055] S1.3, after the reaction is completed, filter the reaction liquid to remove insoluble impurities; perform rotary evaporation of the filtrate at 60°C at a speed of 150 rpm, and then place it in a vacuum drying box for drying treatment, with a drying temperature of 50°C and a drying time of 8 h, to obtain the etherified rhodioloside.

[0056] Example 3: A bio-technology-based anti-fatigue high-efficiency low-sugar sports drink and a preparation method thereof, comprising the following steps:

[0057] S2.1, respectively weigh the following components: 80 parts by weight of water, 5 parts by weight of etherified rhodioloside, 8 parts by weight of hawthorn extract, 6 parts by weight of green tea extract, 3 parts by weight of guarana extract, 8 parts by weight of medlar extract, 20 parts by weight of erythritol, 1 part by weight of sodium chloride, 0.5 parts by weight of potassium chloride, 2 parts by weight of isocitric acid, 1 part by weight of gamma-aminobutyric acid, 1 part by weight of L-ornithine, 0.5 parts by weight of coenzyme Q10, 0.5 parts by weight of choline, and 0.5 parts by weight of sodium carboxymethyl cellulose;

[0058] S2.2, add 70% water in the container, add etherified rhodiolin, hawthorn extract, green tea extract, guarana extract, and medlar extract in sequence, stir at 150 rpm at 20℃ for 15 min; then add erythritol, sodium chloride, and potassium chloride, continue to stir at 150 rpm for 10 min, to obtain a mixed solution;

[0059] S2.3, add isocitric acid to the mixed solution, stir at 200 rpm for 10 min, adjust the pH to 4.0; then add gamma-aminobutyric acid, L-ornithine, coenzyme Q10, and choline, stir at 200 rpm for 15 min until evenly distributed;

[0060] S2.4, add a 2% carboxymethyl cellulose sodium solution to the mixed solution in S2.3, stir at 300 rpm for 30 min; then add 30% water, stir at 400 rpm for 15 min; after mixing evenly, filter, and then perform homogenization treatment by a high-pressure homogenizer, at a pressure of 60 MPa, for 3 cycles, to obtain a bio-technology-based anti-fatigue high-efficiency low-sugar sports drink.

[0061] The preparation method of the etherified rhodiolin is as follows:

[0062] S1.1, respectively weigh the following components: 15 parts by weight of rhodiolin, 24 parts by weight of methyl glucoside, 3 parts by weight of polyethylene glycol-phosphorylcholine, and 5 parts by weight of potassium carbonate;

[0063] S1.2, under nitrogen protection, dissolve the rhodiolin and methyl glucoside in deionized water, stir at 200 rpm for 40 min, and add a 5% potassium carbonate solution to adjust the pH value of the solution to 9; slowly add the polyethylene glycol-phosphorylcholine at a rate of 2 parts by weight per minute, and place the reaction system in a constant-temperature water bath at 60℃, and stir at 400 rpm for 20 h;

[0064] S1.3, after the reaction is completed, filter the reaction liquid to remove insoluble impurities; place the filtrate in a rotary evaporator at 60℃ and stir at 150 rpm, then place it in a vacuum drying box for drying treatment, at a drying temperature of 50℃ for 8 h, to obtain the etherified rhodiolin.

[0065] Example 4: A bio-technology-based anti-fatigue high-efficiency low-sugar sports drink and a preparation method thereof, comprising the following steps:

[0066] S2.1, respectively, take the following weight parts of components: water 80 parts by weight, etherified rhodioloside 5 parts by weight, hawthorn extract 8 parts by weight, green tea extract 6 parts by weight, guarana extract 3 parts by weight, medlar extract 8 parts by weight, erythritol 20 parts by weight, sodium chloride 1 part by weight, potassium chloride 0.5 parts by weight, isocitric acid 2 parts by weight, gamma-aminobutyric acid 1 part by weight, L-ornithine 1 part by weight, coenzyme Q10 0.5 parts by weight, choline 0.5 parts by weight, sodium carboxymethyl cellulose 0.5 parts by weight;

[0067] S2.2, add 70% water in the container, add etherified rhodioloside, hawthorn extract, green tea extract, guarana extract, medlar extract in turn, stir at 20℃ with the speed of 150rpm for 15min; add erythritol, sodium chloride and potassium chloride, continue to stir at the speed of 150rpm for 10min, get mixed solution;

[0068] S2.3, add isocitric acid to the mixed solution, stir at the speed of 200rpm for 10min, adjust pH to 4.0; add gamma-aminobutyric acid, L-ornithine, coenzyme Q10 and choline, stir at the speed of 200rpm for 15min until uniform distribution;

[0069] S2.4, add 2% sodium carboxymethyl cellulose solution to the mixed solution in S2.3, stir at the speed of 300rpm for 30min; add 30% water, stir at the speed of 400rpm for 15min; after mixing evenly, filter, then carry out homogenization treatment by high pressure homogenizer, the pressure is 60MPa, cycle 3 times, get anti-fatigue high-efficiency sugar-reduced sports drink based on biotechnology.

[0070] The preparation method of etherified rhodioloside is as follows:

[0071] S1.1, respectively, take the following weight parts of components: rhodioloside 15 parts by weight, methyl glucoside 27 parts by weight, polyethylene glycol-phosphorylcholine 3 parts by weight, potassium carbonate 5 parts by weight;

[0072] S1.2, under the protection of nitrogen, dissolve rhodioloside and methyl glucoside in deionized water, stir at the speed of 200rpm for 40min, add 5% potassium carbonate solution at the same time to adjust the pH value of the solution to 9; slowly add polyethylene glycol-phosphorylcholine at the speed of 2 parts by weight per minute, and put the reaction system in a constant temperature water bath at 60℃, stir at the speed of 400rpm for 20h;

[0073] S1.3, after the reaction, the reaction liquid is filtered to remove insoluble impurities; the filtrate is rotary evaporated at 60℃ at a speed of 150 rpm, and then dried in a vacuum drying box, the drying temperature is 50℃, and the drying time is 8h, to obtain etherified rhodioside.

[0074] Example 5: A bio-technology-based anti-fatigue high-efficiency low-sugar sports drink and a preparation method, comprising the following steps:

[0075] S2.1, respectively weigh the following components: water 90 parts by weight, etherified rhodioside 5 parts by weight, hawthorn extract 11 parts by weight, green tea extract 9 parts by weight, guarana extract 5 parts by weight, wolfberry extract 11 parts by weight, erythritol 25 parts by weight, sodium chloride 2 parts by weight, potassium chloride 1.2 parts by weight, isocitric acid 3.5 parts by weight, gamma-aminobutyric acid 2 parts by weight, L-ornithine 2 parts by weight, coenzyme Q10 1.2 parts by weight, choline 1.2 parts by weight, sodium carboxymethyl cellulose 1.2 parts by weight;

[0076] S2.2, add 70% water in a container, and then add etherified rhodioside, hawthorn extract, green tea extract, guarana extract and wolfberry extract in turn, and stir at 20℃ at a speed of 150 rpm for 15 min; then add erythritol, sodium chloride and potassium chloride, and continue to stir at a speed of 150 rpm for 10 min to obtain a mixed solution;

[0077] S2.3, add isocitric acid to the mixed solution, and stir at a speed of 200 rpm for 10 min to adjust the pH to 4.0; then add gamma-aminobutyric acid, L-ornithine, coenzyme Q10 and choline, and stir at a speed of 200 rpm for 15 min until evenly distributed;

[0078] S2.4, add a 2% sodium carboxymethyl cellulose solution to the mixed solution in S2.3, and stir at a speed of 300 rpm for 30 min; then add 30% water, and stir at a speed of 400 rpm for 15 min; after mixing evenly, filter, and then perform homogenization treatment by a high-pressure homogenizer, the pressure is 60MPa, and the cycle is 3 times, to obtain a bio-technology-based anti-fatigue high-efficiency low-sugar sports drink.

[0079] The preparation method of the etherified rhodioside is as follows:

[0080] S1.1, respectively weigh the following components: rhodioside 15 parts by weight, methyl glucoside 24 parts by weight, polyethylene glycol-phosphorylcholine 6 parts by weight, potassium carbonate 8 parts by weight;

[0081] S1.2, under the protection of nitrogen, dissolve the rhodioloside and methyl glucoside in deionized water, stir at a speed of 200 rpm for 40 min, add a 5% potassium carbonate solution to adjust the pH value of the solution to 9, slowly add polyethylene glycol-phosphoryl choline at a speed of 2 parts by weight per minute, and place the reaction system in a constant temperature water bath at 60°C, stir at a speed of 400 rpm for 20 h;

[0082] S1.3, after the reaction is completed, the reaction liquid is filtered to remove insoluble impurities; the filtrate is rotary evaporated at 60°C at a speed of 150 rpm, and then dried in a vacuum drying box, the drying temperature is 50°C, and the drying time is 8 h, to obtain etherified rhodioloside.

[0083] Example 6: A bio-technology-based anti-fatigue high-efficiency low-sugar sports drink and a preparation method thereof, comprising the following steps:

[0084] S2.1, respectively weigh the following components: water 90 parts by weight, etherified rhodioloside 8 parts by weight, hawthorn extract 11 parts by weight, green tea extract 9 parts by weight, guarana extract 5 parts by weight, wolfberry extract 11 parts by weight, erythritol 25 parts by weight, sodium chloride 2 parts by weight, potassium chloride 1.2 parts by weight, iso-citric acid 3.5 parts by weight, gamma-aminobutyric acid 2 parts by weight, L-ornithine 2 parts by weight, coenzyme Q10 1.2 parts by weight, choline 1.2 parts by weight, sodium carboxymethyl cellulose 1.2 parts by weight;

[0085] S2.2, add 70% water in a container, and then add etherified rhodioloside, hawthorn extract, green tea extract, guarana extract, and wolfberry extract, stir at a speed of 150 rpm at 20°C for 15 min; then add erythritol, sodium chloride and potassium chloride, continue to stir at a speed of 150 rpm for 10 min, to obtain a mixed solution;

[0086] S2.3, add iso-citric acid to the mixed solution, stir at a speed of 200 rpm for 10 min, and adjust the pH to 4.0; then add gamma-aminobutyric acid, L-ornithine, coenzyme Q10 and choline, and stir at a speed of 200 rpm for 15 min until they are evenly distributed;

[0087] S2.4, add a 2% sodium carboxymethyl cellulose solution to the mixed solution in S2.3, stir at a speed of 300 rpm for 30 min; then add 30% water, stir at a speed of 400 rpm for 15 min; after mixing evenly, filter, and then perform homogenization treatment by a high-pressure homogenizer, the pressure is 60 MPa, and the cycle is 3 times, to obtain a bio-technology-based anti-fatigue high-efficiency low-sugar sports drink.

[0088] The preparation method of the etherified rhodiolin is as follows:

[0089] S1.1, respectively, take the following weight parts of components: rhodiolin 15 parts by weight, methyl glucoside 24 parts by weight, polyethylene glycol-phosphorylcholine 6 parts by weight, potassium carbonate 8 parts by weight;

[0090] S1.2, under the protection of nitrogen, dissolve rhodiolin and methyl glucoside in deionized water, stir at a speed of 200 rpm for 40 min, and add a 5% potassium carbonate solution to adjust the pH value of the solution to 9; slowly add polyethylene glycol-phosphorylcholine at a speed of 2 parts by weight per minute, and place the reaction system in a constant temperature water bath at 60°C, and stir at a speed of 400 rpm for 20 h;

[0091] S1.3, after the reaction is completed, the reaction liquid is filtered to remove insoluble impurities; the filtrate is rotary evaporated at 150 rpm at 60°C, and then dried in a vacuum drying box, with a drying temperature of 50°C and a drying time of 8 h, to obtain etherified rhodiolin.

[0092] Example 7: A high-efficiency anti-fatigue low-sugar sports drink based on biotechnology and a preparation method thereof, comprising the following steps:

[0093] S2.1, respectively, take the following weight parts of components: water 90 parts by weight, etherified rhodiolin 10 parts by weight, hawthorn extract 11 parts by weight, green tea extract 9 parts by weight, guarana extract 5 parts by weight, wolfberry extract 11 parts by weight, erythritol 25 parts by weight, sodium chloride 2 parts by weight, potassium chloride 1.2 parts by weight, isocitric acid 3.5 parts by weight, gamma-aminobutyric acid 2 parts by weight, L-ornithine 2 parts by weight, coenzyme Q10 1.2 parts by weight, choline 1.2 parts by weight, sodium carboxymethylcellulose 1.2 parts by weight;

[0094] S2.2, add 70% water in a container, and sequentially add etherified rhodiolin, hawthorn extract, green tea extract, guarana extract, and wolfberry extract, and stir at a speed of 150 rpm at 20°C for 15 min; then add erythritol, sodium chloride, and potassium chloride, and continue to stir at a speed of 150 rpm for 10 min to obtain a mixed solution;

[0095] S2.3, add isocitric acid to the mixed solution, and stir at a speed of 200 rpm for 10 min to adjust the pH to 4.0; then add gamma-aminobutyric acid, L-ornithine, coenzyme Q10, and choline, and fully stir at a speed of 200 rpm for 15 min until they are evenly distributed;

[0096] S2.4, 2% sodium carboxymethyl cellulose solution is added to the mixed solution in S2.3, stirring at a speed of 300 rpm for 30 min; then 30% water is added, stirring at a speed of 400 rpm for 15 min; after mixing uniformly, filtering is carried out, and then homogenization treatment is carried out through a high-pressure homogenizer, the pressure is 60 MPa, and the cycle is 3 times, to obtain the bio-technology-based anti-fatigue high-efficiency low-sugar sports beverage.

[0097] The preparation method of the etherified rhodioloside is as follows:

[0098] S1.1, the following components are weighed respectively: 15 parts by weight of rhodioloside, 24 parts by weight of methyl glucoside, 6 parts by weight of polyethylene glycol-phosphorylcholine, and 8 parts by weight of potassium carbonate;

[0099] S1.2, under the protection of nitrogen, the rhodioloside and the methyl glucoside are dissolved in deionized water, stirring at a speed of 200 rpm for 40 min, while adding a 5% potassium carbonate solution to adjust the pH value of the solution to 9; the polyethylene glycol-phosphorylcholine is slowly added at a speed of 2 parts by weight per minute, and the reaction system is placed in a constant temperature water bath at 60 DEG C, and stirring is carried out at a speed of 400 rpm for 20 h;

[0100] S1.3, after the reaction is completed, the reaction liquid is filtered to remove insoluble impurities; the filtrate is rotary evaporated at 60 DEG C at a speed of 150 rpm, and then dried in a vacuum drying box, the drying temperature is 50 DEG C, and the drying time is 8 h, to obtain the etherified rhodioloside.

[0101] Comparative Example 1

[0102] The method of Example 7 is adopted, and the etherified rhodioloside is not used, and the rhodioloside is directly used to prepare the bio-technology-based anti-fatigue high-efficiency low-sugar sports beverage.

[0103] Comparative Example 2

[0104] The method of Example 7 is adopted, and the polyethylene glycol-phosphorylcholine is removed in the preparation method of the etherified rhodioloside.

[0105] The bio-technology-based anti-fatigue high-efficiency low-sugar sports beverage prepared by the etherified rhodioloside has the following performance index test items and test standards:

[0106] The anti-fatigue effect is determined through animal experiments: rats are selected, a certain dose of the sports beverage is given, and then the animals are allowed to exercise intensively for 30 minutes; immediately after the exercise, blood samples are collected, serum or plasma is separated by using a centrifuge, and the blood lactic acid concentration is determined by using an enzyme method; a lower blood lactic acid concentration tends to indicate that a substance has better anti-fatigue effect.

[0107] The sample is diluted according to a certain proportion, the insoluble substances are removed by filtration, a series of standard solutions with known concentrations are prepared to cover the expected sample concentration range; the standard solutions are analyzed using a high performance liquid chromatography system, the peak area or peak height is recorded, and the standard curve is drawn; the treated sample is injected into the HPLC system, and the chromatogram and peak area or peak height are recorded; the concentration of the active ingredient in the sample is calculated according to the standard curve.

[0108] The anti-fatigue high-efficiency low-sugar sports drinks prepared in Examples 1-7 and Comparative Examples 1-2 are tested according to the above standard, and the data obtained are shown in Table 1:

[0109] Table 1 Performance data of anti-fatigue high-efficiency low-sugar sports drinks of Examples 1-7 and Comparative Examples 1-2

[0110]

[0111] As can be seen from Examples 1-4, when the mass ratio of salidroside to methyl glucoside in the etherified salidroside gradually increases, the content of active ingredients and the anti-fatigue efficacy of the anti-fatigue high-efficiency low-sugar sports drink gradually increase, but when the mass ratio of salidroside to methyl glucoside reaches a certain value, the content of active ingredients and the anti-fatigue efficacy of the anti-fatigue high-efficiency low-sugar sports drink gradually decrease. Therefore, with the increase of the mass ratio of methyl glucoside, the content of active ingredients and the anti-fatigue efficacy of the anti-fatigue high-efficiency low-sugar sports drink are improved, but excessive increase may reduce the content of active ingredients and the anti-fatigue efficacy of the anti-fatigue high-efficiency low-sugar sports drink;

[0112] Salidroside is the main active ingredient of Rhodiola, which has a unique chemical structure and physiological activity. It can promote the body's utilization of oxygen, improve the energy metabolism level of cells, enhance the function of mitochondria, and promote the synthesis of adenosine triphosphate, thereby providing more energy for muscle contraction and physical movement. In addition, salidroside also has significant antioxidant effects, which can increase the activity of antioxidant enzymes such as superoxide dismutase and glutathione peroxidase, reduce the content of lipid peroxidation products such as malondialdehyde, and reduce the damage of free radicals to cells. Methyl glucoside, as another active ingredient in Rhodiola, can participate in the process of sugar metabolism, optimize the synthesis and decomposition of glycogen, maintain the stability of blood glucose level, and ensure the continuous supply of energy during exercise.

[0113] With the increase of the mass ratio of rhodioloside and methyl glucoside, their regulation on energy metabolism is more significant, which can better meet the body's energy demand during exercise and delay the production of fatigue. A large number of free radicals are produced during exercise, and excessive free radicals will attack biological macromolecules in cells, leading to impaired cell function and triggering fatigue. Rhodioloside can effectively scavenge free radicals, reduce oxidative stress damage, and thus improve the anti-fatigue effect by increasing the activity of antioxidant enzymes and reducing the content of lipid peroxidation products. Methyl glucoside may also have certain antioxidant capacity or synergistically enhance the function of the antioxidant system with rhodioloside. When the mass ratio of the two increases, the overall antioxidant capacity of the sports drink is enhanced, which can effectively scavenge free radicals, reduce oxidative stress damage, and thus improve the anti-fatigue effect.

[0114] Further, as can be seen from the comparison of Examples 5-7, when the proportion of etherified rhodioloside gradually increases while other components remain unchanged, the content of active ingredients and the anti-fatigue effect of the anti-fatigue high-efficiency low-sugar sports drink gradually increase. Therefore, etherified rhodioloside may have a stabilizing effect on other active ingredients in the drink. When its proportion increases, it can better protect other active ingredients, reduce their degradation or inactivation during storage and use, and thus maintain a high level of total active ingredients in the drink that can play a role. Etherified rhodioloside may have stronger antioxidant properties. As its proportion increases, it can more effectively scavenge free radicals in the body, reduce oxidative damage, and protect the normal function of cells and tissues. In addition, as the proportion of etherified rhodioloside increases, its regulation on the nervous endocrine system may be more significant. It may regulate neural endocrine pathways such as the hypothalamic-pituitary-adrenal axis, allowing the body to better adapt to stress during exercise and maintain homeostasis. At the same time, it may also affect the secretion and transmission of neurotransmitters, such as increasing the release of dopamine and norepinephrine, improving brain excitability and body movement ability, and reducing fatigue.

[0115] As can be seen from the comparison of Example 7 and Comparative Example 1, when rhodioloside is not etherified and is directly used to prepare an anti-fatigue high-efficiency low-sugar sports drink based on biotechnology, the content of active ingredients and the anti-fatigue effect of the anti-fatigue high-efficiency low-sugar sports drink are significantly reduced.

[0116] As can be seen from the comparison of Example 7 and Comparative Example 2, when polyethylene glycol-phosphorylcholine is removed in the preparation method of etherified rhodioloside, the content of active ingredients and the anti-fatigue effect of the anti-fatigue high-efficiency low-sugar sports drink are significantly reduced.

[0117] The polyethylene glycol-phosphorylcholine may play a role of a reaction intermediate or catalyst in the synthesis reaction of etherified salidiloside, which helps to improve the reaction rate and completeness, thereby increasing the generation amount of etherified salidiloside, and its presence may have a protective effect on the stability of etherified salidiloside, reducing the occurrence of degradation or isomerization and other side reactions, and ensuring the content of the final active ingredient; the polyethylene glycol-phosphorylcholine may also promote the absorption of etherified salidiloside, which can help etherified salidiloside better pass through the gastrointestinal mucosa into the blood circulation, thereby improving its absorption efficiency in the body and anti-fatigue efficacy; in addition, the polyethylene glycol-phosphorylcholine itself has a certain antioxidant capacity, or can synergistically enhance the antioxidant effect with etherified salidiloside, more effectively clearing free radicals and reducing oxidative damage during exercise; after removing the polyethylene glycol-phosphorylcholine, the generation amount and absorption efficiency of etherified salidiloside will decrease, and the anti-fatigue efficacy will be significantly weakened; at the same time, the lack of its antioxidant effect may lead to a decrease in the body's ability to clear free radicals and an increase in oxidative stress, which is easy to cause fatigue.

[0118] The above shows and describes the basic principles, main features and advantages of the present application. Those skilled in the art should understand that the present application is not limited by the above examples, and the above examples and descriptions in the specification are only preferred examples of the present application and are not intended to limit the present application. Without departing from the spirit and scope of the present application, various changes and improvements can be made to the present application, and these changes and improvements all fall within the scope of the claimed present application. The scope of protection of the present application is defined by the appended claims and their equivalents.

Claims

1. A method for preparing a biotechnology-based anti-fatigue, high-efficiency, low-sugar sports drink, characterized in that, The preparation method is as follows: S2.1 Weigh the following components by weight: 80-100 parts water, 5-10 parts etherified rhodioloside, 8-15 parts hawthorn extract, 6-12 parts green tea extract, 3-8 parts guarana extract, 8-15 parts wolfberry extract, 20-30 parts erythritol, 1-3 parts sodium chloride, 0.5-2 parts potassium chloride, 2-5 parts acidulant, 1-3 parts γ-aminobutyric acid, 1-3 parts L-ornithine, 0.5-2 parts coenzyme Q10, 0.5-2 parts choline, and 0.5-2 parts sodium carboxymethyl cellulose. The etherified rhodioloside is prepared by etherification of rhodioloside with methyl glucoside and the addition of polyethylene glycol-phosphorylcholine. S2.2 Add 60-70% water to a container, then add etherified rhodioloside, hawthorn extract, green tea extract, guarana extract, and wolfberry extract in sequence. Stir at 100-150 rpm for 10-15 minutes at 15-20℃. Then add erythritol, sodium chloride, and potassium chloride, and continue stirring at 100-150 rpm for 5-10 minutes to obtain a mixed solution. S2.3 Add the acidulant to the mixed solution and stir at 100-200 rpm for 5-10 min to adjust the pH to 3.5-4.5; then add γ-aminobutyric acid, L-ornithine, coenzyme Q10 and choline, and stir thoroughly at 100-200 rpm for 10-15 min until evenly distributed. S2.4 Add sodium carboxymethyl cellulose solution to the mixed solution in S2.3 and stir at 200-300 rpm for 15-30 min; then add 30-40% water and stir at 300-400 rpm for 10-15 min; after mixing evenly, filter and then homogenize using a high-pressure homogenizer to obtain a biotechnology-based anti-fatigue high-efficiency sugar-reducing sports drink. The preparation method of the etherified rhodioloside is as follows: S1.1 Weigh the following components by weight: 10-20 parts rhodioloside, 15-30 parts methyl glucoside, 3-8 parts polyethylene glycol-phosphorylcholine, and 5-12 parts potassium carbonate. S1.2 Under nitrogen protection, dissolve rhodioloside and methyl glucoside in deionized water and stir at 100-200 rpm for 30-60 min. At the same time, add potassium carbonate solution to adjust the pH of the solution. Slowly add polyethylene glycol-phosphorylcholine and place the reaction system in a constant temperature water bath at 40-60℃ and stir at 300-400 rpm for 10-24 h. S1.3 After the reaction is complete, the reaction solution is filtered to remove insoluble impurities; the filtrate is then rotary evaporated at 50-60℃ at a speed of 100-150 rpm, and then dried in a vacuum drying oven to obtain etherified rhodioloside. In step S1.2, the pH of the potassium carbonate solution is adjusted to 9-10.

2. The preparation method of the anti-fatigue, high-efficiency, low-sugar sports drink based on biotechnology according to claim 1, characterized in that, In step S1.2, the concentration of the potassium carbonate solution is 5%-10%.

3. The preparation method of the anti-fatigue, high-efficiency, low-sugar sports drink based on biotechnology according to claim 1, characterized in that, In step S1.2, polyethylene glycol-phosphorylcholine is added slowly at a rate of 1-2 parts by weight per minute.

4. The preparation method of the anti-fatigue, high-efficiency, low-sugar sports drink based on biotechnology according to claim 1, characterized in that, In step S1.3, the vacuum drying temperature is 40-50℃ and the drying time is 4-8h.

5. The preparation method of the anti-fatigue, high-efficiency, low-sugar sports drink based on biotechnology according to claim 1, characterized in that, In S2.3, the acidulant is either citric acid or isocitric acid.

6. The preparation method of the anti-fatigue, high-efficiency, low-sugar sports drink based on biotechnology according to claim 1, characterized in that, In step S2.4, the sodium carboxymethyl cellulose solution is obtained by dissolving sodium carboxymethyl cellulose in water at 60-70°C to obtain a sodium carboxymethyl cellulose solution with a concentration of 1%-2%.

7. The preparation method of the anti-fatigue, high-efficiency, low-sugar sports drink based on biotechnology according to claim 1, characterized in that, In S2.4, the pressure of the high-pressure homogenizer is 50-60 MPa, and the number of cycles is 1-3.

Images