Traditional Chinese medicine composition for treating zearalenone poisoning of pigs and application thereof

Abstract

The invention discloses a traditional Chinese medicine composition for treating zearalenone poisoning of pigs and application of the traditional Chinese medicine composition, and belongs to the technical field of traditional Chinese medicines. The traditional Chinese medicine composition comprises the following raw materials in parts by weight: 4-8 parts of geranium wilfordii, 3-5 parts of hyperforin perforatum, 5-10 parts of polygonum aviculare, 1-3 parts of cinnamon and 2-5 parts of hawthorn leaves. According to the traditional Chinese medicine composition, geranium wilfordii and hyperforin perforatum are used as monarch drugs, hawthorn leaves are used as ministerial drugs, polygonum aviculare and cinnamon are used as conductant drugs, both exterior and interior are treated, and the five drugs are applied to the five internal organs to raise spleen qi, relieve liver yang to treat exterior, purge heart fire, tonify kidney yin to treat interior, borrow the force of lung to settle down and enable heart and kidney to intersect, so that the curing effect is achieved. Experiments prove that the prepared traditional Chinese medicine composition can effectively relieve pig ZEA poisoning, the cure rate can reach 96.67%, and a new thought is provided for preventing and treating pig ZEA poisoning.

Description

Technical Field

[0001] The invention relates to the technical field of traditional Chinese medicine, and in particular to a traditional Chinese medicine composition for treating zearalenone poisoning in pigs and an application thereof. Background Art

[0002] Zearalenone (ZEA) is an estrogen-like nonsteroidal mycotoxin produced by Fusarium fungi such as F. graminearum. It has a stable structure and is not easy to decompose. It is usually detected in mold-contaminated grains such as corn, wheat, barley, oats and sorghum. It is one of the most widely distributed mycotoxins. The International Cancer Agency classifies ZEA as a Class III carcinogen. China stipulates that the maximum residue of ZEA in grains and their products is 60 μg / kg. ZEA produces cytotoxicity, genotoxicity, immunotoxicity and reproductive toxicity to livestock. According to literature records, pigs are the most sensitive livestock species to ZEA. ZEA can cause problems such as intestinal and organ inflammation and decreased immune function. In addition, ZEA has a chemical structure similar to estrogen and can bind to estrogen receptors, affecting the reproductive function of pigs, such as causing abortion and false estrus in sows and low sperm motility in boars. After entering the body, ZEA is rapidly absorbed by the intestines and metabolized in the liver, resulting in damage to organs such as the liver, kidneys, spleen, uterus, and testicles. Currently, most studies focus on the toxicity of ZEA to mice. For example, ZEA can reduce the semen quality of male mice, increase the apoptosis rate of spermatogenic cells, and hinder spermatogenesis, thereby damaging male reproductive function.

[0003] However, the above contents currently focus more on scientific research, and most of the experimental animals are mice. The data and research progress obtained are more theoretical. In the actual production process, incidents of zearalenone poisoning in pigs occur frequently, causing serious economic losses. The above contents do not fundamentally solve the problem of the harm caused by zearalenone to pigs. Summary of the invention

[0004] The purpose of the present invention is to provide a Chinese medicine composition for treating ZEA poisoning in pigs and its application, so as to solve the problems existing in the above-mentioned prior art. Experimental verification shows that the Chinese medicine composition prepared by the present invention can effectively alleviate ZEA poisoning in pigs, and the cure rate can reach 100%, which provides a new idea for preventing and treating ZEA poisoning in pigs.

[0005] To achieve the above object, the present invention provides the following solutions:

[0006] The invention provides a traditional Chinese medicine composition for treating zearalenone poisoning in pigs. The composition comprises the following raw materials in parts by weight: 4-8 parts of geranium, 3-5 parts of Hypericum perforatum, 5-10 parts of Polygonum multiflorum, 1-3 parts of cinnamon bark and 2-5 parts of hawthorn leaves.

[0007] Optionally, the Chinese medicine composition comprises the following raw materials in parts by weight: 8 parts of geranium, 3 parts of Hypericum perforatum, 5 parts of Polygonum multiflorum, 2 parts of cinnamon bark and 5 parts of hawthorn leaves.

[0008] The present invention also provides a method for preparing the Chinese medicine composition, comprising the following steps:

[0009] Weigh geranium, Hypericum perforatum, Polygonum multiflorum, cinnamon bark and hawthorn leaves according to weight to obtain a Chinese medicine mixture, add water to boil, and filter to obtain a first filtrate and medicinal residues;

[0010] The residue is decocted again with water until the edge of the extract is gelatinized, and the second filtrate is obtained by filtering;

[0011] The first filtrate and the second filtrate were combined and concentrated to obtain the product.

[0012] Optionally, the weight ratio of the Chinese medicine mixture to water is 1:(5-20), and the decoction time is 1.5 hours.

[0013] Optionally, the mass ratio of the medicinal residue to water is 1:1.

[0014] The present invention also provides application of the traditional Chinese medicine composition in preparing medicine for preventing and / or treating zearalenone poisoning in pigs.

[0015] The present invention also provides a medicine for preventing and / or treating zearalenone poisoning in pigs, comprising the traditional Chinese medicine composition.

[0016] Optionally, the drug is administered at a dosage of 100 mL / kg and is administered twice a day.

[0017] Optionally, the method of taking the drug includes oral administration.

[0018] Optionally, the dosage form of the drug includes a liquid dosage form.

[0019] The present invention discloses the following technical effects:

[0020] The traditional Chinese medicine composition provided by the invention uses geranium and forsythia suspensa as main medicines, hawthorn leaf as assistant medicine, and radix polygoni multiflori and cinnamon bark as guiding medicines, and treats both the exterior and the interior. The five medicines are applied to the five internal organs, so as to raise spleen qi and relieve liver yang to treat the exterior, and to purge heart fire and replenish kidney yin to treat the interior, and utilize the descending power of the lung to make the heart and kidney communicate with each other, so as to achieve the effect of healing.

[0021] Experimental verification shows that the Chinese medicine composition prepared by the present invention can effectively alleviate ZEA poisoning in pigs, with a cure rate of up to 96.67%, providing a new idea for preventing and treating ZEA poisoning in pigs. DETAILED DESCRIPTION

[0022] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as limiting the present invention, but should be understood as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0023] It should be understood that the terms described in the present invention are only for describing a particular embodiment and are not intended to limit the present invention. In addition, for the numerical range in the present invention, it should be understood that each intermediate value between the upper and lower limits of the scope is also specifically disclosed. The intermediate value in any stated value or stated range, and each smaller range between any other stated value or intermediate value in the described range is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded in the scope.

[0024] Unless otherwise indicated, all technical and scientific terms used herein have the same meanings as those generally understood by those skilled in the art. Although the present invention describes only preferred methods and materials, any methods and materials similar or equivalent to those described herein may also be used in the implementation or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials associated with the documents. In the event of a conflict with any incorporated document, the content of this specification shall prevail.

[0025] It will be apparent to those skilled in the art that various modifications and variations may be made to the specific embodiments of the present invention description without departing from the scope or spirit of the present invention. Other embodiments derived from the present invention description will be apparent to those skilled in the art. The present invention description and examples are exemplary only.

[0026] The words “include,” “including,” “have,” “contain,” etc. used in this document are open-ended terms, meaning including but not limited to.

[0027] The "Gezhi Yu Lun" points out that "when a person is alive, the heart is fire, located at the top, and the kidney is water, located at the bottom; water can rise and fire can fall, one rises and the other falls, without end, so life exists." The interaction between the heart and the kidney is also called the harmony of water and fire. The heart is located at the upper part of the body, which is yang and belongs to fire in the five elements; the kidney is located at the lower part of the body, which is yin and belongs to water in the five elements. The two are interdependent and restrictive. "Suwen·Liuweizhi Dalun" says "under the minister fire, water vapor supports it" and "under the monarch fire, yin essence supports it." The heart fire descends to the kidney and warms the kidney yin together with the kidney yang, so that the kidney water is not cold. The kidney water goes up to the heart and nourishes the heart yang together with the heart yin, so that the heart fire is not too strong. If the kidney does not have the heart fire, the water will be cold, and if the heart does not have the kidney water, the fire will be hot. In addition, the heart governs blood, and the kidney governs the storage of essence. The two have the same origin, both of which come from food and water, and can nourish and transform each other. If the essence is sufficient, the blood will be prosperous, and if the essence is deficient, the blood will be deficient. Therefore, "The Treatise on the Origin and Symptoms of Various Diseases" says: "Essence is formed by blood." Moreover, the heart is the sovereign fire, and the kidney is the secondary fire. "If the life fire is hidden, the heart yang will be sufficient, and if the heart yang is abundant, the secondary fire will also be prosperous." The two are in their respective positions, and the heart and kidney will interact with each other. "The interaction between the heart and kidney depends entirely on the rise and fall." ("Shenzhai's Legacy") The heart yang descends to the right and descends to the kidney water. Therefore, the kidney water contains yang energy with a yin body. This yang energy is the yang root. The yang energy is warm and dispersed, and the yin water rises to the left instead of sinking due to the yang root. However, the mechanism of rise and fall lies entirely in the spleen. “The spleen has the tranquility of Kun and the strength of Qian, so it can cause the yang of the heart and lungs to descend and the yin of the kidneys and liver to rise, thus forming the state of heaven and earth.” (Gezhi Yu Lun) The liver belongs to wood and the kidney belongs to water. Water can produce wood. Kidney yin can nourish liver yin and prevent liver yang from rising.

[0028] Zearalenone is mainly derived from Fusarium graminearum, which is a type of mold. Mold is only produced in places with heavy moisture, so it is believed that zearalenone belongs to the water element in the five elements. When pigs eat feed contaminated with zearalenone, they are actually introducing water into their bodies. Introducing water into the body first damages the spleen and stomach. The spleen belongs to the earth, and the earth originally controls water. However, pigs are originally aquatic animals, so they are easily burdened by water. After eating for a long time, the water is extremely abundant and insults the spleen and earth, resulting in spleen deficiency, inability to rise, sinking of the middle qi, lack of power to transport and movement, and accumulation of water and moisture, and long-term diarrhea. Weak spleen qi, poor transportation and transformation, and the inability of water and grain essence to nourish the kidney can lead to insufficient kidney essence, loss of kidney qi, malnutrition of the testicles, and then impotence; loss of essence and blood, inability to reveal the soul, and water does not contain wood, so the liver yang rises, resulting in blindness; kidney essence is damaged, kidney yin is insufficient, and kidney yang is lacking in source, so there is no water to rise, and the heart yang cannot be restrained, and then the heart fire is too hyperactive, resulting in impaired consciousness. In summary, this syndrome can be explained by drawing water into the body, which in turn insults the spleen and earth, causing spleen qi to sink, kidney yin deficiency, blindness due to loss of yin, excessive heart yang, and hyperactivity of liver yang.

[0029] This prescription contains five herbs. Considering the economic cost, the herbs are cheap and easy to obtain, which is the premise of large-scale implementation. This prescription specifically contains 4-8 parts of geranium, 3-5 parts of Hypericum perforatum, 5-10 parts of Polygonum multiflorum, 1-3 parts of cinnamon bark, and 2-5 parts of hawthorn leaves. The key is to strengthen the spleen and earth, raise the spleen qi, purge the heart fire, and replenish the kidney yin, so that the yang qi of the whole body rises on the left and falls on the right, and the heart and kidney interact to strengthen the body. The prescription is explained as follows: Geranium and Hypericum perforatum are the main herbs. Both are bitter, bitter belongs to fire, enter the heart, can purge the heart fire, and calm the mind. Geranium tastes spicy and bitter, and is flat in nature. It has the effects of dispelling wind and unblocking collaterals, clearing heat and detoxifying. It can relieve liver yang, clear heat and activate blood circulation when it enters the liver meridian; it can stop chronic dysentery, thicken the intestines and stomach, regulate the middle and strengthen the spleen when it enters the spleen meridian; it can strengthen the tendons and bones and dredge the collaterals when it enters the kidney meridian. Hypericum perforatum tastes bitter and astringent, and is neutral in nature. It enters the liver meridian, clears away heat and detoxifies, relieves liver depression, and relieves liver yang hyperactivity. The astringent taste is astringent and can also stop diarrhea. When the spleen qi does not rise, the essence and blood rush downward and the yin is lost. The "Nanjing" says: "Those who lose yin will be blind." The symptom of losing yin is actually a superficial phenomenon. The root cause is that the heart yang is excessive and the liver yang is hyperactive. Therefore, Geranium and Hypericum perforatum are first used to drain the yang of the heart and liver, stop diarrhea, and improve eyesight to treat the surface. Hawthorn leaves are used as ministers to assist. They are sour and neutral in nature. The sour taste belongs to wood, enters the liver, and has the effect of regulating qi and unblocking meridians. Polygonum multiflorum and cinnamon are both messengers. Polygonum multiflorum is bitter and cold, enters the bladder meridian and large intestine meridian, can promote diuresis and relieve stranguria, and regulate water channels. The lung and large intestine are exterior and interior, and Polygonum multiflorum acts on the large intestine meridian, which can also promote the lung to play the role of spreading and descending. "The heart and kidney are not connected. After all, the kidney water is drying up and the heart fire is inflamed. Most of them are due to yin deficiency" ("Selected Medical Theories of Fuxi"). Cinnamon is spicy and sweet, and enters the kidney and spleen meridians. "Suwen. Theory of Storing Qi and the Way of Time" says: "The kidney is dry, so eat spicy food to moisten it." Now that kidney yin is weak, the spicy taste of cinnamon can moisten the kidney, enter the kidney meridian to warm the kidney yang, and lead the fire back to the origin; the spleen desires sweetness, so its sweet taste nourishes the spleen, cultivates the spleen earth to prosper in all seasons, and then makes the heart and kidney connect to treat the root cause, and achieves the function of water and fire being in harmony.

[0030] This prescription treats both the exterior and the interior. The five herbs are applied to the five internal organs, raising spleen qi and soothing liver yang to treat the exterior, and purging heart fire and replenishing kidney yin to treat the interior. It uses the lung's descending power to enable the heart and kidney to communicate, thus achieving a healing effect.

[0031] Example 1

[0032] A Chinese medicine composition for treating zearalenone poisoning in pigs, comprising the following raw materials:

[0033] 80g of geranium, 30g of Hypericum perforatum, 50g of Polygonum multiflorum, 20g of cinnamon bark and 50g of hawthorn leaf.

[0034] Preparation method: weigh the raw materials according to the above raw material formula and mix them to obtain a Chinese medicine mixture, put it into a medicine pot, add water 10 times the total weight, heat to boiling, boil for 1.5 hours, filter, and obtain a first filtrate; add an equal weight of water to the residue, heat to boiling again and boil again, boil until the edge of the extract is gelatinized, filter, and obtain a second filtrate; combine the first filtrate and the second filtrate, and concentrate to 200 mL to obtain a medicinal solution.

[0035] Dosage and Administration: Oral administration; 100mL / kg each time, once in the morning and once in the evening.

[0036] Example 2

[0037] A Chinese medicine composition for treating zearalenone poisoning in pigs, comprising the following raw materials:

[0038] 50g of geranium, 30g of Hypericum perforatum, 50g of Polygonum multiflorum, 10g of cinnamon bark and 30g of hawthorn leaf.

[0039] Preparation method: weigh the raw materials according to the above raw material formula and mix them to obtain a Chinese medicine mixture, put it into a medicine pot, add water 5 times the total weight, heat to boiling, boil for 1.5 hours, filter, and obtain a first filtrate; add an equal weight of water to the residue, heat to boiling again and boil again, boil until the edge of the extract is gelatinized, filter, and obtain a second filtrate; combine the first filtrate and the second filtrate, and concentrate to 200 mL to obtain a medicinal solution.

[0040] Dosage and Administration: Oral administration; 100mL / kg each time, once in the morning and once in the evening.

[0041] Example 3

[0042] A Chinese medicine composition for treating zearalenone poisoning in pigs, comprising the following raw materials:

[0043] 60g of geranium, 40g of Hypericum perforatum, 60g of Polygonum multiflorum, 10g of cinnamon bark and 20g of hawthorn leaf.

[0044] Preparation method: weigh the raw materials according to the above raw material formula and mix them to obtain a Chinese medicine mixture, put it into a medicine pot, add water 20 times the total weight, heat to boiling, boil for 1.5 hours, filter, and obtain a first filtrate; add an equal weight of water to the residue, heat to boiling again and boil again, boil until the edge of the extract is gelatinized, filter, and obtain a second filtrate; combine the first filtrate and the second filtrate, and concentrate to 200 mL to obtain a medicinal solution.

[0045] Dosage and Administration: Oral administration; 100mL / kg each time, once in the morning and once in the evening.

[0046] Example 4

[0047] A Chinese medicine composition for treating zearalenone poisoning in pigs, comprising the following raw materials:

[0048] 40g of geranium, 50g of Hypericum perforatum, 100g of Polygonum multiflorum, 30g of cinnamon bark and 40g of hawthorn leaf.

[0049] Preparation method: weigh the raw materials according to the above raw material formula and mix them to obtain a Chinese medicine mixture, put it into a medicine pot, add water 10 times the total weight, heat to boiling, boil for 1.5 hours, filter, and obtain a first filtrate; add an equal weight of water to the residue, heat to boiling again and boil again, boil until the edge of the extract is gelatinized, filter, and obtain a second filtrate; combine the first filtrate and the second filtrate, and concentrate to 200 mL to obtain a medicinal solution.

[0050] Dosage and Administration: Oral administration; 100mL / kg each time, once in the morning and once in the evening.

[0051] Comparative Example 1

[0052] A traditional Chinese medicine composition for treating zearalenone poisoning in pigs, comprising the same raw materials as those used in Example 1, except that the weight portions of the raw materials are greatly different: the weight portions of geranium, Hypericum perforatum and cinnamon bark are increased, and the weight portions of Polygonum multiflorum and Crataegus pinnatifida are decreased, specifically: 100 g geranium, 90 g Hypericum perforatum, 20 g Polygonum multiflorum, 50 g cinnamon bark and 10 g Crataegus pinnatifida.

[0053] Preparation method: weigh the raw materials according to the above raw material formula and mix them to obtain a Chinese medicine mixture, put it into a medicine pot, add water 10 times the total weight, heat to boiling, boil for 1.5 hours, filter, and obtain a first filtrate; add an equal weight of water to the residue, heat to boiling again and boil again, boil until the edge of the extract is gelatinized, filter, and obtain a second filtrate; combine the first filtrate and the second filtrate, and concentrate to 200 mL to obtain a medicinal solution.

[0054] Dosage and Administration: Oral administration; 100mL / kg each time, once in the morning and once in the evening.

[0055] Comparative Example 2

[0056] A traditional Chinese medicine composition for treating zearalenone poisoning in pigs, which differs from Example 1 in that cinnamon is replaced by cloves, specifically: 80g of geranium, 30g of Hypericum perforatum, 50g of Polygonum multiflorum, 20g of cloves and 50g of hawthorn leaves.

[0057] Preparation method: weigh the raw materials according to the above raw material formula and mix them to obtain a Chinese medicine mixture, put it into a medicine pot, add water 10 times the total weight, heat to boiling, boil for 1.5 hours, filter, and obtain a first filtrate; add an equal weight of water to the residue, heat to boiling again and boil again, boil until the edge of the extract is gelatinized, filter, and obtain a second filtrate; combine the first filtrate and the second filtrate, and concentrate to 200 mL to obtain a medicinal solution.

[0058] Dosage and Administration: Oral administration; 100mL / kg each time, once in the morning and once in the evening.

[0059] Application Examples

[0060] 1. Experimental Animals

[0061] In July 2023, a large number of pigs in a farm in Heilongjiang Province suffered from diarrhea, vomiting, and decreased feed intake. In severe cases, corneal clouding and death even occurred. After testing, the zearalenone content in the feed was seriously exceeded, so it was diagnosed as zearalenone poisoning.

[0062] 2. Experimental drugs

[0063] The experimental animals were divided into 8 groups and isolated and raised, namely a blank group (pigs not poisoned by zearalenone), a model group (pigs poisoned by zearalenone), two control groups and four treatment groups (pigs poisoned by zearalenone were treated by taking drugs: oral administration, 100 mL / kg each time, once in the morning and evening), 30 pigs in each group, including 15 male and female pigs. The control group was treated with the drugs prepared in Comparative Examples 1-2, and the four treatment groups were treated with the drugs prepared in Examples 1-4.

[0064] 3. Experimental methods

[0065] All pigs were fed regularly and had free access to water. The experimental period was 14 days, with a course of treatment of 7 days each, for a total of 2 courses. The selected experimental pigs were all kept in the same pig house, which was clean, dry, well ventilated, and at a suitable temperature (15-25°C). During the experiment, feeding and management were carried out in accordance with the unified requirements of the pig farm.

[0066] 4. Detection indicators

[0067] During the experiment, the diarrhea and blindness of each group of pigs, the degree of edema of the foreskin of boars and the degree of swelling of the vulva of sows were recorded, the malondialdehyde (MDA) content and glutathione peroxidase (GSH-Px) content in the serum were detected, and the cure rate was calculated.

[0068] Among them, the efficacy criteria for diarrhea, blindness, boar foreskin edema, and sow vulva swelling are as follows:

[0069] (1) “—”: no symptoms;

[0070] (2) “+”: mild symptoms but not obvious, and not affecting daily activities and production performance;

[0071] (3) “++”: symptoms exist, affecting daily activities and interfering with production performance;

[0072] (4) “+++”: There are obvious symptoms, which seriously affect the daily activities of animals and significantly reduce production performance.

[0073] The malondialdehyde content and glutathione peroxidase content were determined using kits, and the malondialdehyde content determination kit and the glutathione peroxidase content determination kit were purchased from Nanjing Jiancheng Bioengineering Institute.

[0074] 5. Results

[0075] The experimental results are shown in Table 1. It can be seen that the Chinese medicine composition prepared by the embodiment of the present invention can effectively alleviate ZEA poisoning in pigs, and the cure rate can reach 100%.

[0076] Table 1 Effects of each group on the production performance of ZEA poisoning pigs

[0077]

[0078]

[0079] Note: Compared with the same column, different letters on the shoulders indicate significant differences (P<0.05), and the same letters on the shoulders indicate no significant differences (P>0.05).

[0080] The embodiments described above are only descriptions of the preferred modes of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary technicians in this field should all fall within the protection scope determined by the claims of the present invention.

Claims

1. A Chinese medicine composition for treating zearalenone poisoning in pigs, characterized in that: The invention comprises the following raw materials in parts by weight: 4-8 parts of geranium, 3-5 parts of Hypericum perforatum, 5-10 parts of Polygonum multiflorum, 1-3 parts of cinnamon bark and 2-5 parts of hawthorn leaves.

2. The Chinese medicine composition according to claim 1, characterized in that The traditional Chinese medicine composition comprises the following raw materials in parts by weight: 8 parts of geranium, 3 parts of Hypericum perforatum, 5 parts of Polygonum multiflorum, 2 parts of cinnamon bark and 5 parts of hawthorn leaves.

3. The method for preparing the Chinese medicine composition according to claim 1 or 2, characterized in that: The following steps are involved: Weigh geranium, Hypericum perforatum, Polygonum multiflorum, cinnamon bark and hawthorn leaves according to weight to obtain a Chinese medicine mixture, add water to boil, and filter to obtain a first filtrate and medicinal residues; The residue is decocted again with water until the edge of the extract is gelatinized, and the second filtrate is obtained by filtering; The first filtrate and the second filtrate were combined and concentrated to obtain the product.

4. The preparation method according to claim 3, characterized in that: The weight ratio of the Chinese medicine mixture to water is 1:(5-20), and the decoction time is 1.5 hours.

5. The preparation method according to claim 3, characterized in that: The mass ratio of the medicinal residue to water is 1:

1.

6. Use of the Chinese medicine composition as claimed in claim 1 or 2 in the preparation of a medicament for preventing and / or treating zearalenone poisoning in pigs.

7. A drug for preventing and / or treating zearalenone poisoning in pigs, characterized in that: Including the Chinese medicine composition according to claim 1 or 2.

8. The drug according to claim 7, characterized in that The dosage of the drug is 100 mL / kg; the administration frequency is 2 times a day.

9. The drug according to claim 7, characterized in that The method of taking the drug includes oral administration.

10. The drug according to claim 7, characterized in that The dosage form of the drug includes a liquid dosage form.

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