Application of Canxin oral liquid in preparation of anti-aging drugs

Abstract

The invention relates to an application of a Canxin oral liquid in preparation of an anti-aging drug, and belongs to the field of medicines. The Canxin oral liquid can tonify kidney and replenish essence, invigorate spleen and replenish qi, nourish blood and activate blood, and can effectively improve spleen and kidney deficiency, deficiency of qi and blood, soreness and weakness of waist and knees, insomnia and amnesia and weakness after illness caused by aging. Modern medicine proves that the Canxin oral liquid has the functions of improving blood sugar and blood fat levels of middle-aged and elderly diabetics and inhibiting occurrence and development of atherosclerotic plaques, animal experiment research proves that the Canxin oral liquid has a good treatment effect on aging model rats, can remarkably increase organ indexes and reduce oxidative stress and inflammation related factors, and has a good application prospect. The pathological forms of the liver and the kidney are improved, and a good anti-aging effect is achieved.

Description

Technical Field

[0001] The invention belongs to the field of medicine, and in particular relates to an application of Kangxin oral liquid in the preparation of anti-aging drugs. Background Art

[0002] Aging refers to the phenomenon that the body's physiological and psychological adaptability to the environment is progressively reduced and gradually tends to death. It is the inevitable result of the combined effects of many pathological, physiological and psychological processes. It is a biological and psychological process in the final stage of individual growth and development. According to modern medical theory, the aging process will cause aging of reproductive organs, neurodegeneration and dementia, disorders of gastrointestinal microbiota and intestinal immune system, decreased autonomous function of hematopoietic cells and osteoporosis, decreased memory and executive function

[50] . By linking traditional Chinese medicine theory with modern medical theory, we can systematically and deeply understand the specific changes in various aspects of aging, which is conducive to determining research indicators for aging.

[0003] At present, the treatment of aging includes medical aesthetics and chemical drug treatment. Medical aesthetics include photorejuvenation, Thermage, etc. However, it can only improve the surface aging phenomenon to a certain extent, and may cause skin allergies and recurrence. Chemical drug treatment includes dasatinib and metformin, etc. These drugs have positive effects on cardiovascular diseases, hypertension, cancer and neurodegenerative diseases. However, they have the disadvantages of single action site and potential side effects. In recent years, traditional Chinese medicine has begun to emerge in the application of anti-aging, and has attracted more and more attention with its unique multi-target effect, good tolerance, low toxicity and price advantage.

[0004] In traditional Chinese medicine, it is believed that kidney deficiency, spleen and stomach deficiency, heart deficiency and liver deficiency will lead to aging. The kidney is the innate foundation and can be said to be the driving force of human life; the spleen and stomach are the acquired foundation, and the essence of water and grain enter the stomach; the liver stores blood and is responsible for the release of qi; and the essence is the source of human life activities. These are all important parts of the human body and maintaining health. When these parts are deficient, it will cause the body to be unbalanced and unable to work normally, which will be reflected in the overall aging. Studies have shown that drugs with the effects of replenishing the kidney and replenishing essence, strengthening the spleen and replenishing qi, nourishing blood and promoting blood circulation can effectively delay aging. In clinical practice, drugs for replenishing essence and replenishing the kidney, replenishing qi and strengthening the spleen, and promoting blood circulation and nourishing blood often use wolfberry, Polygonum multiflorum, Salvia miltiorrhiza, etc.

[0005] Kangxin Oral Liquid (Kangxin Koufuye) National Drug Standard, standard number: WS-5027(B-0027)-2014Z, the prescription composition includes: 13 Chinese medicinal herbs: 36 grams of Cuscuta seeds, 36 grams of Polygonatum sibiricum, 36 grams of Chrysanthemum, 27 grams of Astragalus, 27 grams of Crataegus, 24 grams of Angelica sinensis, 18 grams of Lycium barbarum, 18 grams of Processed Polygonum multiflorum, 18 grams of Epimedium, 18 grams of Ligustrum lucidum, 18 grams of Salvia miltiorrhiza, 18 grams of Ziziphus jujuba seeds and 18 grams of Rehmannia root. In the prescription, wolfberry has the effects of nourishing the kidney, moistening the lung, nourishing the liver, and improving eyesight; processed Polygonum multiflorum has the effects of nourishing the liver and kidney, benefiting the essence and blood, blackening the hair, and strengthening the tendons and bones; Epimedium has the effects of nourishing the kidney and strengthening yang, dispelling wind and removing dampness; Ligustrum lucidum has the effects of nourishing the liver and kidney, strengthening the waist and knees, improving eyesight and blackening hair; Cuscuta has the effects of nourishing the liver and kidney, consolidating essence and reducing urine, calming the fetus, improving eyesight, and stopping diarrhea; Astragalus has the effects of strengthening the spleen and replenishing the middle, raising yang and lifting the sunken, benefiting the defense and consolidating the exterior, diuresis, and supporting poison and promoting muscle regeneration; Polygonatum (processed) has the effects of nourishing qi and nourishing yin, strengthening the spleen, moistening the lungs, and benefiting the kidney; Salvia miltiorrhiza has the effects of promoting blood circulation and removing blood stasis, dredge menstruation and relieving pain, clearing the heart and removing troubles, cooling blood and eliminating carbuncle; Rehmannia has the effects of clearing away heat and cooling blood. All the medicines are used together to play the effects of nourishing the kidney and replenishing essence, strengthening the spleen and replenishing qi, nourishing blood and promoting blood circulation. Kangxin oral liquid is a brown-yellow solution, with a small amount of precipitate that is easily dispersed after being left for a long time; it has a slight fragrance, spicy and slightly sweet taste. It has the effects of nourishing the kidney and replenishing essence, strengthening the spleen and replenishing qi, nourishing blood and activating blood circulation. It can be used for symptoms such as fatigue, soreness of waist and knees, insomnia and forgetfulness, and physical weakness after illness caused by deficiency of both spleen and kidney and insufficient qi and blood. Summary of the invention

[0006] The invention provides an application of Kangxin oral liquid in the preparation of anti-aging drugs, aiming at increasing new drug uses of the drug.

[0007] The technical solution adopted by the present invention is: application of Kangxin oral liquid in the preparation of anti-aging drugs.

[0008] Under the intervention of Kangxin oral liquid, the levels of oxidative stress and inflammation-related factors in aging model rats were significantly reduced.

[0009] Under the intervention of Kangxin oral liquid, the organ indexes and pathological morphology of the liver and kidneys were also effectively improved.

[0010] The advantages of the present invention are: in traditional Chinese medicine, it is believed that kidney deficiency, spleen and stomach deficiency, heart deficiency and liver deficiency will lead to aging. The kidney is the innate foundation and can be said to be the driving force of human life; the spleen and stomach are the acquired foundation, and the essence of water and grain all enter the stomach; the liver stores blood and is responsible for dredging; essence is the source of human life activities. These are all important parts of the human body composition and health maintenance. When these parts are deficient, it will cause the body to be unbalanced and unable to work normally, which is reflected in the whole body as aging. Studies have shown that drugs with the effects of tonifying the kidney and filling essence, strengthening the spleen and replenishing qi, nourishing blood and activating blood circulation can effectively delay aging. Kangxin oral liquid tonifies the kidney and fills essence, strengthens the spleen and replenishes qi, nourishes blood and activates blood circulation, and can timely and significantly improve the symptoms of fatigue, soreness of waist and knees, insomnia and forgetfulness, and weakness after illness caused by spleen and kidney deficiency and insufficient qi and blood in the aging process, and improve the quality of life.

[0011] Modern medical research has proven that Kangxin Oral Liquid has the function of improving blood sugar and blood lipid levels in middle-aged and elderly diabetic patients and inhibiting the occurrence and development of atherosclerotic plaques. Animal experimental studies have shown that it has a good therapeutic effect on aging model rats, and can significantly reduce the levels of oxidative stress and inflammation-related factors in aging model rats, and effectively improve organ indexes and the pathological morphology of the liver and kidneys. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] Figure 1 is the graph of daily body weight changes of rats in each group;

[0013] Figure 2 is the graph of changes in organ indexes of rats in each group;

[0014] Figure 3 is the change graph of oxidative stress indexes and inflammatory factors in the blood of rats in each group;

[0015] Figure 4 is the change diagram of oxidative stress indexes and inflammatory factors in brain tissue of rats in each group;

[0016] Figure 5 is the change diagram of oxidative stress indexes and inflammatory factors in spleen tissue of rats in each group;

[0017] Figure 6 is the change diagram of oxidative stress indexes and inflammatory factors in liver tissue of rats in each group;

[0018] Figure 7 is the change diagram of oxidative stress indexes and inflammatory factors in kidney tissue of rats in each group;

[0019] Figure 8 are H&E staining photos of liver section samples of rats in each group;

[0020] Fig. 9 These are H&E staining photos of kidney section samples from rats in each group. DETAILED DESCRIPTION

[0021] Application of Kangxin oral liquid in the preparation of anti-aging drugs.

[0022] Under the intervention of Kangxin oral liquid, the levels of oxidative stress and inflammation-related factors in aging model rats were significantly reduced.

[0023] Under the intervention of Kangxin oral liquid, the organ indexes and pathological morphology of the liver and kidneys were also effectively improved.

[0024] The effects of the present invention are further described below through experimental examples.

[0025] Experimental example: The intervention effect of Kangxin oral liquid on aging model rats

[0026] 1.1 Experimental Materials

[0027] 1.1.1 Drugs and reagents. The test samples and main reagents are shown in Table 1.

[0028] Table 1 Drugs and reagents

[0029]

[0030] 1.1.2 Instruments and Equipment

[0031] The main instruments are shown in Table 2.

[0032] Table 2 Main experimental instruments

[0033]

[0034] 1.1.3 Experimental animals

[0035] SPF SD rats (female, 6 months old, weighing 200 g ± 20 g) were purchased from Changchun Yisi Laboratory Animal Technology Co., Ltd. (Batch No.: 202200044263). Natural light cycle lighting (12 hours of light / dark alternation) was used, the temperature was 25 °C ± 2 °C, and the relative humidity was 60% ± 5%. Relevant animal experiments were carried out in accordance with the "Guide for the care and use of laboratory animals" and the requirements of the Ethics Committee of the School of Pharmacy of Jilin University.

[0036] 1.1.4 Preparation of main solutions

[0037] Modeling drug D-galactose solution: Use a balance to accurately weigh a certain amount of D-galactose, dissolve it with physiological saline to an appropriate concentration, and after it is completely dissolved, filter it with a filter membrane to sterilize it and set it aside.

[0038] Metformin solution: Grind the metformin hydrochloride tablets and dissolve them completely in sterile water for later use.

[0039] Kangxin Oral Liquid: Dilute Kangxin Oral Liquid with sterile water by 2 times and 4 times respectively for later use.

[0040] 1.2 Experimental methods

[0041] 1.2.1 Modeling and drug administration

[0042] SD male rats that need to establish an aging model are intraperitoneally injected with D-galactose solution (400 mg / kg) daily, and the blank control group is intraperitoneally injected with normal saline for 8 consecutive weeks. Mice in the blank control group and the model group are gavaged with distilled water, and other groups are gavaged with corresponding drugs. After fasting for 12 hours at the end of the 8th week, the rats were killed on the second day. During the experiment, the basic conditions of the experimental rats were carefully observed and recorded in detail every day, including weight changes, activity, mental state, and glossiness of fur. The symptoms of weight loss, mental depression, and withered fur after intraperitoneal injection of D-galactose solution were used as a sign of the success of establishing the experimental model.

[0043] 1.2.2 Weight Record

[0044] During the experiment, all rats were weighed every day, their general behavioral states were observed, and their weight changes were recorded every day.

[0045] 1.2.3 Sample collection and pretreatment

[0046] Serum samples: At the end of the 8th week of the experiment, the mice were fasted for 12 h, and whole blood samples were obtained through the aorta. After standing at 4°C for 30 min, the blood samples were centrifuged at 4000 r / min for 10 min to collect serum samples, which were stored at -80°C for future use.

[0047] Brain tissue, spleen, liver and kidney samples: Organ tissues were separated and accurately weighed, and the organ coefficient was calculated according to the following formula: Organ coefficient = organ mass (g) / rat body weight (g) * 100%.

[0048] 1.2.4 Detection of oxidative stress index factors and inflammatory factors in various parts

[0049] Each rat (8 ml / kg) was anesthetized by intraperitoneal injection of a small animal anesthetic, and blood was collected from the abdominal aorta and allowed to stand for half an hour before centrifugation to obtain the upper serum. After weighing each tissue and organ, a certain amount of tissue of the same weight was cut, added with 9 times the volume of normal saline, and then ground and centrifuged to obtain the supernatant for ELISA determination of SOD, MDA, IL-1β and IL-6.

[0050] 1.2.5 Effect of Kangxin Oral Liquid on the Pathological Morphology of Liver and Kidney in Aging Rats

[0051] Each rat (8 ml / kg) was anesthetized by intraperitoneal injection of a special anesthetic for small animals. The same part of each required tissue was fixed in 10% neutral formalin for 24 hours, then embedded in paraffin and sectioned. H﹠E staining was performed, and pathological changes of liver tissue were observed under a light microscope and photographs were taken.

[0052] 1.2.6 Data Statistics and Analysis

[0053] GraphPad Prism 10.0 software (GraphPad software, CA) was used to analyze the experimental results. All data were expressed as mean ± standard deviation (± SD). The t-test was used for comparison between the two groups, and one-way analyses of variance (ANOVA) was used for multiple comparisons. p < 0.05 was considered statistically significant.

[0054] 1.3 Experimental Results

[0055] 1.3.1 Effect of Kangxin Oral Liquid on Body Weight of Rats

[0056] like Figure 1 As shown, compared with the normal group, the weight gain trend of the rats in the model group slowed down significantly during the 8-week period. Compared with the weight in the last week, the average weight of the model group was 3.84% lower than that of the blank group (p<0.05), indicating that the model of accelerated aging of rats caused by intraperitoneal injection of D-galactose was successfully established. Compared with the model group, the weight gain trend of the three groups treated with Kangxin oral liquid was better. In the eighth week, the high-dose group increased by 27.27% (p<0.01). This shows that Kangxin oral liquid can significantly alleviate the loss of appetite caused by aging and the subsequent slowing of weight gain. The weight changes of rats in each group are shown in Table 3.

[0057] Table 3 Body weight changes of rats in each group ( n=10)

[0058]

[0059] 1.3.2 Effects of Kangxin Oral Liquid on Organ Indexes of Rats

[0060] like Figure 2As shown, compared with the blank group, the brain, spleen, liver and kidney indexes of the model group decreased to varying degrees, with the brain index decreasing by 7.49% (p<0.05), the spleen index decreasing by 10.45% (p<0.01), the liver index decreasing by 4.14% (p<0.05), and the kidney index decreasing by 4% (p<0.05), indicating that the aging model was successfully established. Compared with the model group, the high-dose group of Kangxin oral liquid increased the brain index by 8.98% (p<0.01), the spleen index by 14.72% (p<0.01), the liver index by 10.14% (p<0.01), and the kidney index by 6.94% (p<0.01). The low-dose and medium-dose groups were slightly improved compared with the model group, but there was no significant difference. This shows that high-dose Kangxin oral liquid can effectively alleviate the condition of organ atrophy or degenerative lesions caused by aging, verifying the anti-aging pharmacological activity of Kangxin oral liquid. At the same time, this part also compared the damage to the liver and kidneys of the positive drug and the high-dose Kangxin oral liquid group after long-term use. It was found that the liver and kidney conditions of the high-dose Kangxin oral liquid group were better than those of the positive drug group (p<0.01, p<0.05). This shows that when taken for a long time, Kangxin oral liquid has less burden on the liver and kidneys and is more suitable for long-term use. It is an ideal long-term anti-aging drug. The organ indexes of rats in each group are shown in Table 1.4.

[0061] Table 4 Changes of organ indexes of rats in each group ( n=10)

[0062]

[0063] 1.3.3 Effects of Kangxin oral liquid on oxidative stress indicators and inflammatory factors in various tissues of aging rats

[0064] like Figures 3 to 7As shown, this study compared the differences in SOD activity, MDA content, IL-6 content and IL-1β content in serum, brain, spleen, liver and kidney tissues, and explored the differences in the effects of Kangxin oral solution on different organs during the aging process. First, the differences in various indicators in serum were compared. Compared with the blank group, the SOD activity in serum decreased by 16.66% (p<0.01), the MDA content increased by 22.77% (p<0.01), the IL-6 content increased by 20.31% (p<0.01), and the IL-1β content increased by 30.51% (p<0.01), indicating that the D-galactose-induced rapid aging model in rats was successfully established, and the oxidative stress and inflammatory response of rats in the model group increased significantly. After administration, the Kangxin oral liquid treatment group significantly adjusted the abnormalities of various indicators caused by aging. Compared with the model group, the SOD activity in the serum of the high-dose group increased by 16.4% (p<0.01), the MDA content decreased by 15.8% (p<0.01), the IL-6 content decreased by 13.1% (p<0.01), and the IL-1β content decreased by 15.7% (p<0.01). It was verified that Kangxin oral liquid has a good anti-aging effect, and its anti-aging activity in the blood is dose-dependent.

[0065] In the brain tissue, compared with the blank group, the SOD activity in the brain tissue decreased by 39% (p<0.01), the MDA content increased by 133.65% (p<0.01), the IL-6 content increased by 151.6% (p<0.01), and the IL-1β content increased by 61.09% (p<0.01), indicating that the D-galactose-induced rapid aging model of rats was successfully established, and the brain aging of the rats in the model group was obvious. After administration, the Kangxin oral liquid treatment group significantly adjusted the abnormalities of various indicators caused by aging. Compared with the model group, the SOD activity in the brain tissue of the high-dose group increased by 39.2% (p<0.01), the MDA content decreased by 53.3% (p<0.01), the IL-6 content decreased by 53.4% ​​(p<0.01), and the IL-1β content decreased by 28.5% (p<0.01). These results indicate that Kangxin oral liquid may be able to act on the brain through the blood-brain barrier, delay brain aging, and its anti-aging activity in the brain is dose-dependent.

[0066] In spleen tissue, compared with the blank group, SOD activity in spleen tissue decreased by 33.48% (p<0.01), MDA content increased by 46.89% (p<0.01), IL-6 content increased by 25.85% (p<0.01), and IL-1β content increased by 110.49% (p<0.01), indicating that the D-galactose-induced rapid aging model of rats was successfully established, and the spleen of rats in the model group was obviously aged. After administration, the Kangxin oral liquid treatment group significantly adjusted the abnormalities of various indicators caused by aging. Compared with the model group, SOD activity in spleen tissue of the high-dose group increased by 25.9% (p<0.01), MDA content decreased by 29.3% (p<0.01), IL-6 content decreased by 13.9% (p<0.01), and IL-1β content decreased by 29.8% (p<0.01). These results indicate that Kangxin oral liquid significantly improves spleen aging, effectively enhances the activity state of the spleen, and its anti-aging activity in the spleen is dose-dependent.

[0067] In liver tissue, compared with the blank group, SOD activity in liver tissue decreased by 46.81% (p<0.01), MDA content increased by 27.93% (p<0.01), IL-6 content increased by 30.55% (p<0.01), and IL-1β content increased by 48.17% (p<0.01), indicating that the D-galactose-induced rapid aging model of rats was successfully established, and the liver of the model group rats was obviously aged. After administration, the Kangxin oral liquid treatment group significantly adjusted the abnormalities of various indicators caused by aging. Compared with the model group, the SOD activity in liver tissue of the high-dose group increased by 69.1%, MDA content decreased by 19% (p<0.01), IL-6 content decreased by 23.2% (p<0.01), and IL-1β content decreased by 21.2% (p<0.01). At the same time, the high-dose group was compared with the positive drug group, and it was found that the SOD activity in the high-dose group increased by 24.71% (p<0.01), and the IL-1β content decreased by 7.03% (p<0.05). The liver is an organ with rich blood flow and is also the main organ of metabolism, so it can be predicted that Kangxin Oral Liquid has a good effect in the liver and is dose-dependent. Compared with the positive drug, it was found that under the condition of long-term use, Kangxin Oral Liquid has less burden on the liver.

[0068] In kidney tissue, compared with the blank group, SOD activity in liver tissue decreased by 37.56%, MDA content increased by 23.78%, IL-6 content increased by 41.55%, and IL-1β content increased by 31.88%, indicating that the D-galactose-induced rapid aging model of rats was successfully established, and the kidneys of rats in the model group were obviously aged. After administration, the Kangxin oral liquid treatment group significantly adjusted the abnormalities of various indicators caused by aging. Compared with the model group, the SOD activity in kidney tissue of the high-dose group increased by 52.1% (p<0.01), MDA content decreased by 17.1% (p<0.01), IL-6 content decreased by 28.9% (p<0.01), and IL-1β content decreased by 15.4% (p<0.01). At the same time, the high-dose group was compared with the positive drug group, and it was found that the SOD activity in the high-dose group increased by 15.16% (p<0.01), the MDA content decreased by 8.1% (p<0.01), and the IL-6 content decreased by 13.5% (p<0.05). These results show that Kangxin oral liquid also has a good anti-aging effect on the kidneys, and it is dose-dependent. At the same time, compared with the positive drug, it was found that under the condition of long-term use, Kangxin oral liquid also has a relatively small burden on the kidneys.

[0069] The specific effects of Kangxin oral liquid on the activity and content of oxidative stress and inflammatory factors in various parts of rats are shown in Tables 5 to 9.

[0070] Table 5 Changes in the activity and content of oxidative stress factors and inflammatory factors in the serum of rats in each group (±SD, n=10)

[0071]

[0072] Table 6 Changes in the activity and content of oxidative stress factors and inflammatory factors in the brain tissue of rats in each group ( n=10)

[0073]

[0074] Table 7 Changes in the activity and content of oxidative stress factors and inflammatory factors in spleen tissue of rats in each group ( n=10)

[0075]

[0076] Table 8 Changes in the activity and content of oxidative stress factors and inflammatory factors in the liver tissue of rats in each group ( n=10)

[0077]

[0078] Table 9 Changes in the activity and content of oxidative stress factors and inflammatory factors in the kidney tissue of rats in each group ( n=10)

[0079]

[0080] 1.3.4 Effect of Kangxin Oral Liquid on Liver and Kidney Pathological Morphology in Aging Rats

[0081] Long-term medication may cause damage to the liver and kidneys, leading to abnormal pathological morphology of the liver and kidneys. The specific manifestations are as follows Figure 8 and 9 As shown. The liver tissue fiber structure of the KXM group and the KXH group was normal, the hepatocyte plate and cord structure was normal, the hepatic lobule was clearly demarcated, the cells were evenly distributed and polygonal, the cell membrane was intact, and the cell nuclei were clearly arranged and visible. No inflammatory cells and red blood cell infiltration were observed. In the Met group, the hepatic lobule boundaries were unclear, the hepatic sinusoids were dilated and congested, and the hepatic sinusoidal gaps were narrowed; the cell plate cords were disordered, the volume was enlarged, swollen, necrotic, turbid, vacuolar degeneration, the nucleus was condensed or fragmented and dissolved; inflammatory cells and red blood cell infiltration were observed.

[0082] The renal tissue fiber structure of the KXM group and the KXH group was normal, with dense and complete edges, clear boundaries between the medulla and cortex, intact tubular epithelium, and normal cell morphology; there were no abnormalities in the collecting ducts and Bowman's capsule, and the nuclei were clearly arranged and visible. No granular degeneration or vacuolar degeneration was found in the renal tubules. No glomerular volume change was found, and no mesangial cell proliferation was found. No inflammatory cell infiltration was found, showing normal renal tissue morphological structural characteristics. The kidneys of the Met group showed structural atrophy and necrosis, cysts appeared in the renal pelvis, tubular epithelium atrophy, and vacuolar deformation of the tubular lumen. The glomerular epithelial cells were necrotic, cell fragments fell off, and the edges were slightly blurred. The glomeruli were atrophic, and the mesangial cells proliferated massively. A large number of inflammatory cell infiltrations appeared.

[0083] The above results show that Kangxin oral liquid is an anti-aging drug that can be taken for a long time and has less burden on the liver and kidneys.

[0084] 1.4 Summary

[0085] This section mainly explores the anti-aging efficacy of Kangxin Oral Liquid in the D-galactose-induced aging rat model, and finds that Kangxin Oral Liquid has good anti-aging activity. It was found that various indicators in brain tissue were also improved, indicating that Kangxin Oral Liquid can improve brain aging through the blood-brain barrier. And compared the differences in the anti-aging activity of Kangxin Oral Liquid on each organ, the results showed that the best site of anti-aging activity of Kangxin Oral Liquid at different concentrations was the brain, followed by the liver, kidney, spleen and blood. This is consistent with the functions of Kangxin Oral Liquid, which mainly invigorates the kidney and replenishes essence, invigorates the spleen and replenishes qi, and nourishes blood and activates blood circulation.

[0086] In addition, it was found that by comparing the physiological indicators and morphological changes of the liver and kidneys, Kangxin oral liquid has less burden on the liver and kidneys than positive drugs under the condition of long-term medication. Traditional Chinese patent medicines usually have mild effects and are suitable for chronic diseases that require long-term medication. Aging is also a slow process. The experimental results show that Kangxin oral liquid can effectively and gently adjust the physiological indicators that change during the aging process, thereby achieving the purpose of delaying aging.

Claims

1. Application of Kangxin oral liquid in the preparation of anti-aging drugs.

2. The use of Kangxin oral liquid according to claim 1 in the preparation of anti-aging drugs, characterized in that: Under the intervention of Kangxin oral liquid, the levels of oxidative stress and inflammation-related factors in aging model rats were significantly reduced.

3. The use of Kangxin oral liquid according to claim 1 in the preparation of anti-aging drugs, characterized in that: Under the intervention of Kangxin oral liquid, the organ indexes and pathological morphology of liver and kidney of aging model rats were also effectively improved.

Images