Traditional Chinese medicine composition for treating coronary heart disease and preparation method thereof

Abstract

The invention discloses a traditional Chinese medicine compound composition for treating coronary heart disease, which is prepared from the following raw materials in parts by weight: 15-40 parts of acanthopanax root, 10-30 parts of gynostemma pentaphylla, 15-30 parts of uniflower swisscentaury root, 5-20 parts of lucid ganoderma, 5-20 parts of turmeric, 5-20 parts of safflower, 5-20 parts of giant knotweed rhizome and 10-30 parts of kudzuvine root, and discloses a preparation method and application of the traditional Chinese medicine compound composition.

Description

Technical Field

[0001] The present application belongs to the field of traditional Chinese medicine, and specifically relates to a traditional Chinese medicine composition for treating coronary heart disease and a preparation method thereof. Background Art

[0002] According to the "China Cardiovascular Health and Disease Report 2023", cardiovascular disease (CVD) has become the leading cause of death among urban and rural residents in my country, and its morbidity and mortality rates are still on the rise. Among them, there are about 11.39 million patients with coronary heart disease (CHD), which is one of the main diseases causing cardiovascular death, seriously affecting the quality of life of patients and increasing their economic burden.

[0003] Atherosclerosis is the main pathological process of coronary heart disease, and its onset is closely related to lipid metabolism disorders. Low-density lipoprotein cholesterol is an independent risk factor for atherosclerosis. Lowering LDL has become the main treatment strategy for preventing and treating AS. Although such methods are very effective, lowering LDL-c only reduces the incidence of AS by 50-60%. There are still many residual risk factors in plaques, and the incidence of adverse cardiovascular events (MACE) is still very high. Many studies have found that while lipids are lowered, there are still many inflammatory burdens in plaques. Abnormal lipid metabolism does not exist independently in the occurrence and development of atherosclerotic plaques. AS patients are accompanied by elevated inflammatory factors, and many inflammatory cytokines and inflammatory mediators are significantly associated with the incidence of coronary heart disease and adverse cardiovascular events. At present, more and more studies have shown that AS is a chronic inflammatory disease.

[0004] Although the role of inflammation in the pathogenesis of AS has been gradually recognized, how to transform it into an effective anti-inflammatory treatment strategy is still full of challenges. The individual differences in atherosclerotic inflammatory pathogenesis are large, the reactions are complex, and there are many targets, including proinflammatory cytokines and inflammatory cascade signaling molecules. Currently, there are no clinical drugs on the market for anti-inflammatory treatment of coronary heart disease. The above studies show that anti-inflammatory treatment is still under exploration, and it is still very important to find new anti-inflammatory pathways.

[0005] Traditional Chinese medicine believes that coronary heart disease can be classified as "chest pain". Most doctors treat coronary heart disease from the perspective of "mutual phlegm and blood stasis". They believe that chest pain and heart pain are often related to the "heart". "Phlegm and blood stasis" is the main pathogenesis of chest pain. Phlegm-resolving and blood-stasis-dispersing methods are the common methods of Chinese medicine to treat chest pain and heart pain. However, pathological changes such as cell necrosis, oxidative stress injury, and inflammatory response in the process of arteriosclerosis seem to be difficult to be summarized by "phlegm turbidity" and "blood stasis". Now a large number of clinical and basic studies have shown that while "activating blood circulation and resolving phlegm", combining the treatment of soothing the liver, invigorating qi, nourishing yin, and clearing heat will achieve more satisfactory results. This shows that coronary heart disease is a disease involving multiple pathogenic factors such as "qi deficiency", "yin deficiency", "blood stasis", and "heat toxicity".

[0006] "Holistic concept" is one of the main characteristics of traditional Chinese medicine. "Holistic concept" is one of the basic characteristics of traditional Chinese medicine. Starting from the overall human organs, the onset of chest pain and heart pain should not be limited to the "heart", but also related to the liver. Treating both the symptoms and the root cause is the concept of traditional Chinese medicine. "Huangdi Neijing·Suwen·Biaobenbingchuanlun" says: "Those who know the symptoms and the root cause will do everything right; those who do not know the symptoms and the root cause will act recklessly." Therefore, in the treatment of coronary heart disease, we must not only see the pathological factors such as phlegm, blood stasis, and heat toxicity, but also take into account the basic pathogenesis of qi and yin deficiency, and take into account both the symptoms and the root cause. "Stagnation and heat" is the main pathogenesis of chest pain and heart pain, which coincides with the interaction between lipid metabolism and inflammation to promote the progression of AS. Traditional Chinese medicine has the advantages of multiple targets and multiple pathways, and plays an important role in improving patients' clinical symptoms, increasing efficacy, reducing side effects, and improving complications. Therefore, the applicant believes that the selection of stasis and heat syndrome for coronary heart disease is critical and has practical significance. It is of great significance to provide a traditional Chinese medicine formula for treating qi and yin deficiency and stasis and heat syndrome of coronary heart disease. Summary of the invention

[0007] The purpose of the present invention is to provide a Chinese medicine composition for significantly treating angina pectoris caused by coronary heart disease, which has the effects of invigorating qi and nourishing yin, clearing away heat, cooling blood and dispersing blood stasis, and can treat coronary heart disease caused by deficiency of both qi and yin in the heart meridian and mutual stasis and heat through lipid-lowering and anti-inflammatory treatment. The Chinese medicine composition of the present invention not only conforms to traditional Chinese medicine theory, but also combines the characteristics of modern coronary heart disease, combines traditional properties and meridians with modern pharmacology, takes both the symptoms and the root causes into consideration, and has a good effect.

[0008] The present invention is achieved by adopting the following technical solutions:

[0009] A Chinese medicinal compound composition for treating coronary heart disease is prepared from the following raw materials in parts by weight: 15-30 parts of radix scutellariae, 5-20 parts of polygonum cuspidatum, 15-40 parts of acanthopanax senticosus, 10-30 parts of gynostemma pentaphyllum, 5-20 parts of turmeric, 5-20 parts of safflower, 5-20 parts of ganoderma lucidum and 10-30 parts of kudzu root.

[0010] The traditional Chinese medicine compound composition for treating coronary heart disease is prepared from the following raw materials in parts by weight: 30 parts of radix scutellariae, 10 parts of polygonum cuspidatum, 30 parts of acanthopanax senticosus, 15 parts of gynostemma pentaphyllum, 10 parts of turmeric, 10 parts of safflower, 10 parts of ganoderma lucidum and 15 parts of kudzu root.

[0011] The preparation method of the Chinese medicinal compound composition for treating coronary heart disease comprises the following steps:

[0012] Step S1: Soak the radix spatholobi, rhizome of knotweed, acanthopanax senticosus, gynostemma pentaphyllum, turmeric, safflower, ganoderma lucidum and kudzu root in water for 1 hour;

[0013] Step S2: adding water and boiling for 2 times, adding 10 times the amount of water for the first time, soaking for 30 minutes, boiling, keeping slightly boiling for 45 minutes, filtering through double-layer gauze to obtain a filtrate; adding 8 times the amount of water to the filter residue and boiling, keeping slightly boiling for 30 minutes, filtering through double-layer gauze to obtain a filtrate, combining the two filtrates, concentrating by rotary evaporation, and adding auxiliary materials to prepare one of oral liquid, tablets, capsules, and granules.

[0014] Application of the Chinese medicinal compound composition for treating coronary heart disease in preparing medicine for treating coronary heart disease.

[0015] The Chinese medicinal compound composition for treating coronary heart disease is used in preparing medicine for treating coronary heart disease, which belongs to the type of deficiency of both qi and yin, and stasis and heat.

[0016] Lophatherum gracile is the dried root of the plant Lophatherum gracile of the Asteraceae family. It is bitter and cold. It enters the stomach meridian. It clears away heat and detoxifies, eliminates carbuncle, promotes lactation, relaxes tendons and dredges the meridians. It is used for carbuncle, sore, scrofula, phlegm nodules, breast carbuncle, swelling and pain, milk blockage, tendon and vein cramps, and joint pain.

[0017] Polygonum cuspidatum is the dried rhizome and root of Polygonum cuspidatum, a plant of Polygonum cuspidatum. It tastes slightly bitter and slightly cold. It enters the liver, gallbladder, and lung meridians. It can dispel dampness and jaundice, clear away heat and detoxify, dissipate blood stasis and relieve pain, and relieve cough and resolve phlegm. It is used for damp-heat jaundice, stranguria, leucorrhea, rheumatic pain, carbuncle, heat toxicity, ulcer, burns caused by water or fire, snake bites, amenorrhea, dysmenorrhea, accumulation of masses, injuries caused by falls, lung heat cough, and heat-induced constipation. Ganoderma lucidum is the dried fruiting body of Ganoderma lucidum or Ganoderma rubrum, a fungus of the Polyporaceae family. It tastes sweet and neutral. It enters the heart, lung, liver, and kidney meridians. It can replenish qi and calm the mind, relieve cough and asthma. It is used for restlessness, insomnia, palpitations, asthenia, shortness of breath, and loss of appetite.

[0018] Acanthopanax senticosus is the dried root and rhizome or stem of the plant Acanthopanax senticosus of the Araliaceae family. It is sweet, slightly bitter, and warm. It enters the spleen, lung, kidney, and heart meridians. It replenishes qi and strengthens the spleen, nourishes the kidney and calms the mind. It is used for lung and spleen deficiency, physical weakness, loss of appetite, lung and kidney deficiency, chronic cough and asthma, kidney deficiency, waist and knee pain, heart and spleen deficiency, and insomnia and dreaminess.

[0019] Gynostemma pentaphyllum is the dried aerial part of the plant Gynostemma pentaphyllum of the Cucurbitaceae family. It is sweet, bitter and cold. It enters the spleen and lung meridians. It replenishes qi and strengthens the spleen, resolves phlegm and relieves cough, clears away heat and detoxifies, and resolves turbidity and reduces fat. It is used for fatigue, poor appetite, lung heat and thick phlegm, cough and asthma, and hyperlipidemia.

[0020] Turmeric is the dried rhizome of the ginger family. It is pungent, bitter and warm. It enters the liver and spleen meridians. It can promote blood circulation and qi circulation, relieve pain. It is used for chest and flank pain, dysmenorrhea, amenorrhea, postpartum abdominal pain, traumatic injuries, bruises and pain, and rheumatic shoulder pain.

[0021] Safflower is the dried flower of the safflower plant of the Asteraceae family. It is pungent and warm. It enters the heart and liver meridians. It promotes blood circulation and menstruation, dissipates blood stasis and relieves pain. It is used for amenorrhea, dysmenorrhea, lumps and masses, chest pain, sores, swelling and pain, and injuries from falls.

[0022] Ganoderma lucidum is the dried fruiting body of the fungus Ganoderma lucidum (Leyss.ex Fr.) Karst. or Ganoderma sinense Zhao, Xu et Zhang of the Polyporaceae family. It has the effects of replenishing qi and calming the mind, relieving cough and relieving asthma. It is mainly used to treat restlessness, insomnia, palpitations, cough, asthma, excessive sputum, and asthenia.

[0023] Pueraria root is the dried root of Pueraria lobata, a plant of the Leguminosae family. It is sweet, pungent and cool. It enters the spleen, stomach and lung meridians. It relieves muscle and fever, promotes the production of body fluid, promotes the exfoliation of rashes, promotes yang and stops diarrhea, promotes blood circulation and activates collaterals, and relieves alcohol poisoning. It is used for exogenous fever and headache, stiff neck and back pain, thirst due to fever, thirst, measles that does not clear up, spleen deficiency diarrhea, dizziness and headache, hemiplegia due to stroke, chest pain and heartache, and alcohol poisoning. A Chinese medicine composition is prepared.

[0024] Beneficial effects of the present invention:

[0025] Coronary heart disease belongs to the category of chest pain and angina in traditional Chinese medicine. The present invention combines the modern characteristics of chest pain and angina, treats both the symptoms and the root cause, and harmonizes the heart and liver to treat chest pain and angina caused by qi and yin deficiency and blood stasis and heat. Most patients with coronary heart disease are elderly people. The Yellow Emperor's Classic of Internal Medicine states that when a person reaches 40, the yin qi is halved, and his daily life is declining. The elderly "often have insufficient yang and excess yin". The liver and kidney yin deficiency lasts for a long time, and the qi and blood source is insufficient, resulting in deficiency of yin and blood in the heart, arterial endothelial dysfunction, insufficient blood vessels, poor circulation of qi and blood, and endogenous blood stasis. Therefore, the treatment of chest pain should consider the basic pathogenesis of qi and yin deficiency of the heart.

[0026] "Holistic concept" is one of the basic characteristics of traditional Chinese medicine. Starting from the overall human organs, the onset of chest pain and angina should not be limited to the "heart", but also related to the liver. The liver is the mother of the heart. The heart governs blood vessels, the liver governs fascia, and the heart valves and ventricles are all components of fascia. At the same time, the pericardium and liver belong to the same meridian. The heart governs blood circulation, the liver regulates blood volume, and the blood circulation of the heart depends on the regulation of the liver. If the liver's dredging and drainage are abnormal, the qi movement may be blocked, which may lead to poor circulation of heart blood or blood cannot be stored normally in the liver, affecting the heart system, causing palpitations, or blood rupture, plaque rupture, blood not flowing through the normal veins, and true heart pain. The pathological reflection is mainly manifested as fascial disharmony, or tension, or spasm, or bleeding. Pathological factors are related to blood stasis, phlegm turbidity, and heat toxicity. In addition, the "blood stasis and heat" pathogenesis of chest pain and angina also manifests in the following aspects: (1) The pathogenic process of blood stasis and heat runs through the stages of AS development. Moreover, modern people’s lifestyle and diet are not regulated. They are fond of fatty and greasy foods, which cause abnormal lipid metabolism. Smoking and drinking can all lead to the internal generation of phlegm and turbidity. The long-term accumulation of phlegm and turbidity will turn into heat, which will torment the blood vessels, increase blood viscosity, and make blood stasis and heat obstructed. The accumulation of heat will aggravate blood stasis, and the stasis and heat will form plaques, resulting in chest pain. Stasis and heat will enter the collaterals for a long time, damage the blood vessels, aggravate the inflammation in the core of arterial plaques, cause plaque rupture, and form "stasis" of blood away from the meridians. (2) The pathological products and risk factors of AS are mostly related to "stasis and heat": the increase of blood viscosity and blood lipids in arterial plaques is non-physiological, turbid blood, which is the category of "stasis". Its risk factors, such as hypertension, hyperlipidemia, diabetes, smoking, etc., all have the pathological mechanism of "heat" causing disease. Gu Songyuan, a doctor in the Qing Dynasty, believed that "smoking is the leader of pungent heat" and diabetes (polydipsia) is a "yang knot" disease. Inflammation plays an important role in the formation of AS and plaque rupture, and the inflammatory response coincides with "stasis and heat"; (3) "stasis and heat" have their pathophysiological basis. Related studies have shown that unstable plaques have a significantly higher temperature than stable plaques because they have more inflammatory cells in their core. Therefore, chest pain and heart pain should be treated from a holistic perspective, and the heart and liver should be treated together.

[0027] The radix polygoni multiflori and polygonum cuspidatum in the prescription are bitter and cold, can clear away heat and detoxify, enter blood and remove blood stasis, can clear liver heat, relieve liver fire, regulate the liver's dredging, relax the meridians and dredge the vessels, clear away heat, activate blood circulation and remove blood stasis, can clear away heat and detoxify, and stop the source of blood stasis, so they are the main medicines; the treatment of diseases must be based on the root cause, the acanthopanax senticosus in the prescription is sweet and slightly bitter, and enters the heart, kidney, spleen, and lung meridians, can invigorate qi, strengthen the spleen and tonify the kidney, the gynostemma pentaphyllum enters the spleen and lung meridians, can tonify the spleen qi, and nourish the blood vessels, and the gynostemma pentaphyllum is bitter and cold, and can also nourish yin and generate The combination of the two can invigorate qi, nourish yin and clear away heat, and they are all assistant drugs; "When qi moves, blood moves, and when qi stagnates, blood stagnates." Turmeric and safflower are pungent and warm, which can not only invigorate blood circulation and relieve pain, promote blood circulation, but also dissipate blood stasis and promote qi circulation; if the qi and yin of the heart meridian are insufficient, the mind will be malnourished. Ganoderma lucidum can not only assist the main drug in invigorating qi, but also calm the mind. Pueraria root is sweet and cool, and can encourage the spleen and stomach to rise in the clear yang qi while clearing heat, and has the function of promoting body fluid while dispersing the stasis and heat in the upper part of the body. The four drugs are all assistant drugs. The combination of all drugs can treat the heart and liver at the same time, taking into account both the symptoms and the root causes, and achieve the effect of "invigorating qi, nourishing yin, invigorating blood circulation and removing blood stasis, clearing heat and detoxifying."

[0028] This prescription establishes the treatment principle of "tonifying qi and nourishing yin, promoting blood circulation and removing blood stasis, clearing away heat and detoxifying" and the treatment concept of treating both the heart and liver and taking both the symptoms and the root cause into consideration. It not only focuses on strengthening the body's health and strengthening the root cause, nourishing the liver and kidney to strengthen the body's health and improve the body's ability to repair itself and resist diseases; it also emphasizes expelling evil spirits, eliminating pathological factors and relieving symptoms by promoting blood circulation and removing blood stasis, clearing away heat and detoxifying. Activating blood circulation and replenishing qi are used together, clearing heat and nourishing yin are used together, promoting blood circulation without hurting the body, and clearing heat without hurting the yin. This balanced concept of treating both the symptoms and the root cause avoids the drawbacks of simply strengthening the body and possibly helping the evil spirits or simply removing the evil spirits and possibly hurting the body, achieving a dynamic balance in the treatment process and improving the overall effect of the treatment. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 Pathological conditions of aortic plaques in mice in each group

[0030] A. Arterial Oil Red O staining results, lipids accumulate in the aorta, and lipids are represented by red. B. Quantification of lipid area percentage. C. HE staining results of aortic sections and Oil Red O staining results of aortic sections. The red arrow indicates the site of lipid accumulation in the vascular intima. The blue arrow indicates the site of lipid accumulation in the vascular intima. The scale bars are 200μm. DETAILED DESCRIPTION

[0031] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0032] Example 1: Take 30g of Rhizoma Lepidium, 10g of Polygonum cuspidatum, 30g of Acanthopanax senticosus, 15g of Gynostemma pentaphyllum, 10g of Curcuma longa, 10g of Carthamus tinctorius, 10g of Ganoderma lucidum, and 15g of Pueraria lobata, and the preparation method comprises the following steps:

[0033] Step S1: Soak Acanthopanax senticosus, Gynostemma pentaphyllum, Rhizoma Cynomorii, Ganoderma lucidum, Curcuma longa, Carthamus tinctorius, Polygonum cuspidatum, and Pueraria root in water for 1 hour;

[0034] Step S2: add water and boil twice, add 10 times the amount of water for the first time, soak for 30 minutes, boil, keep slightly boiling for 45 minutes, filter through double gauze to obtain filtrate; add 8 times the amount of water to the filter residue, boil, keep slightly boiling for 30 minutes, filter through double gauze to obtain filtrate, combine the two filtrates, and prepare an oral solution.

[0035] Example 2: Take 15g of Rhizoma Lepidium, 20g of Polygonum Cuspidati, 15g of Acanthopanax Senticosus, 30g of Gynostemma Pentaphyllum, 5g of Curcuma Longa, 20g of Carthamus Tinctorius, 5g of Ganoderma Lucidum, and 30g of Puerariae Radix, and prepare the mixture by the following steps:

[0036] Step S1: Soak Acanthopanax senticosus, Gynostemma pentaphyllum, Rhizoma Cynomorii, Ganoderma lucidum, Curcuma longa, Carthamus tinctorius, Polygonum cuspidatum, and Pueraria root in water for 1 hour;

[0037] Step S2: add water and boil twice, add 10 times the amount of water for the first time, soak for 30 minutes, boil, keep slightly boiling for 45 minutes, filter through double gauze to obtain a filtrate; add 8 times the amount of water to the filter residue, boil, keep slightly boiling for 30 minutes, filter through double gauze to obtain a filtrate, combine the two filtrates, and prepare into granules according to conventional processes.

[0038] Example 3: Take 30g of Rhizoma Cynomorii, 5g of Polygonum cuspidatum, 40g of Acanthopanax senticosus, 10g of Gynostemma pentaphyllum, 20g of Curcuma longa, 5g of Carthamus tinctorius, 20g of Ganoderma lucidum, and 10g of Pueraria lobata, and prepare the mixture by the following steps:

[0039] Step S1, soaking Acanthopanax senticosus, Gynostemma pentaphyllum, Rhizoma Cynomorii, Ganoderma lucidum, Curcuma longa, Carthamus tinctorius, Polygonum cuspidatum, and Pueraria root in water for 1 hour;

[0040] Step S2, adding water and boiling for 2 times, adding 10 times the amount of water for the first time, soaking for 30 minutes, boiling, keeping slightly boiling for 45 minutes, filtering through double-layer gauze to obtain a filtrate; adding 8 times the amount of water to the filter residue, boiling, keeping slightly boiling for 30 minutes, filtering through double-layer gauze to obtain a filtrate, combining the two filtrates, and preparing into tablets according to conventional processes.

[0041] Example 4: Pharmacodynamics Experiment

[0042] Purpose of the experiment: To observe the pharmacological effects of the Chinese medicine composition by conducting pharmacological experiments on blood lipids, serum inflammatory factors, aortic plaque area, etc. in atherosclerotic ApoE- / - mice using blank group, model group, low-dose group (group a), medium-dose group (group b), high-dose group (group c), and atorvastatin calcium group.

[0043] 1 Effect of the Chinese medicine combination of the present invention on atherosclerosis in ApoE- / - mice with coronary atherosclerosis

[0044] 1.1 Establishment of Apo E- / - mouse atherosclerosis model by high-fat diet feeding

[0045] After 1 week of adaptive feeding, 8-week-old Apo E- / - mice were fed a high-fat diet (1% cholesterol + 10% lard + 10% egg yolk powder + 0.2% pig bile salt + 78.8% basic feed) for 12 weeks for modeling.

[0046] 1.2 Experimental animals and groups

[0047] 6 C57BL / 6 mice, 36 Apo E- / - mice, male, 8 weeks old, weighing 20±3g. ①C57BL / 6 mice were used as the control group and fed with normal feed. ApoE- / - mice were randomly divided into 6 groups, 6 mice in each group, fed with high-fat feed, and the low, medium and high dose groups were given the drug prepared in Example 1 at 0.5 times, 1 times and 2 times the dose of 70kg adult body weight, respectively, specifically divided into: ②Model group; ③Atorvastatin group [A, converted drug concentration 0.13mg / ml gavage]; ④a group [low dose group, drug concentration of 0.4225g raw drug amount / ml]; ⑤b group [medium dose group, drug concentration of 0.845g raw drug amount / ml]; ⑥c group [high dose group, drug concentration of 1.69g raw drug amount / ml]; once a day, continuously administered for 10 weeks, and the gavage volume was 0.2mL / 10g body weight. The mice in the normal group and the model group were given oral administration of 0.2 mL / 10 g body weight of double distilled water every day. Two hours after the last oral administration, blood was collected from the fundus vein and centrifuged to obtain serum. The serum total cholesterol (TC), triglycerides (TG), low-density lipoprotein cholesterol (LDL-c), and high-density lipoprotein cholesterol (HDL-c) were detected by automatic biochemical analyzer. The Elisa method was used to examine the indicators of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interferon-γ (IFN-γ) in the serum. The aortic root was stained with Oil Red O, HE staining, and the whole aorta was stained with Oil Red O to analyze the plaque morphology, structural integrity, fibrous cap thickness, number of necrotic cores and other indicators. The specific experimental results are shown in Tables 1, 2, 3 and Figure 1 .

[0048] Table 1 Aortic plaque area of ​​mice in each group ( n=3)

[0049]

[0050] Compared with the control group, ##P<0.01, #P<0.05; compared with the model group, **P<0.01, *P<0.05.

[0051] Figure 1 The pathological conditions of aortic plaques in each group of mice are shown. Compared with the control group, ##P<0.01, #P<0.05; compared with the model group, **P<0.01, *P<0.05. (A) Lipids accumulate in the aorta, and lipids are represented by red. (B) Quantification of lipid area percentage. (C) HE staining results of aortic sections. The red arrows indicate the sites of lipid accumulation in the vascular intima. The scale bars are 200μm. (D) Oil Red O staining results of aortic sections. The blue arrows indicate the sites of lipid accumulation in the vascular intima. The scale bars are 200μm.

[0052] The results showed that the Chinese herbal compound groups a, b and c could significantly reduce the plaque area of ​​atherosclerotic mice. There was a statistically significant difference between group a and the control group (P < 0.05), and there was a significant statistical difference between group b, group c and atorvastatin calcium group and the control group (P < 0.01).

[0053] Table 2 Blood lipid levels of mice in each group ( n=6)

[0054]

[0055] Compared with the control group, ##P<0.01, #P<0.05; compared with the model group, **P<0.01, *P<0.05.

[0056] The results showed that the Chinese herbal compound group a, group b, and group c could significantly reduce the levels of TC, TG, LDL-c, and HDL-c in mice with atherosclerosis, with significant statistical differences compared with the control group (P < 0.01).

[0057] Table 3 Serum inflammation levels of mice in each group

[0058]

[0059] Compared with the control group, ##P<0.01, #P<0.05; compared with the model group, **P<0.01, *P<0.05.

[0060] The results showed that the Chinese herbal compound group b and group c could significantly reduce the levels of TC, TG, LDL-c and HDL-c in mice with atherosclerosis, with significant statistical differences compared with the control group (P < 0.01).

[0061] In summary, the Chinese medicine combination of the present application can lower blood lipids and serum inflammation levels, reduce the area of ​​atherosclerotic plaques, and its lipid-lowering and anti-inflammatory effects are better than those of the atorvastatin group, reflecting the effectiveness and scientificity of the present application in treating coronary heart disease based on the concept of treating both the heart and the liver.

[0062] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A Chinese medicinal compound composition for treating coronary heart disease, characterized in that: The composition is prepared from the following raw materials in parts by weight: 15-30 parts of radix scutellariae, 5-20 parts of polygonum cuspidatum, 15-40 parts of acanthopanax senticosus, 10-30 parts of gynostemma pentaphyllum, 5-20 parts of turmeric, 5-20 parts of safflower, 5-20 parts of ganoderma lucidum and 10-30 parts of kudzu root.

2. The Chinese medicinal compound composition for treating coronary heart disease according to claim 1, characterized in that: The composition is prepared from the following raw materials in parts by weight: 30 parts of radix scutellariae, 10 parts of polygonum cuspidatum, 30 parts of acanthopanax senticosus, 15 parts of gynostemma pentaphyllum, 10 parts of turmeric, 10 parts of safflower, 10 parts of ganoderma lucidum and 15 parts of kudzu root.

3. The Chinese medicinal compound composition for treating coronary heart disease according to claim 1, characterized in that: The preparation method comprises the following steps: Step S1: Soak the radix spatholobi, rhizome of knotweed, acanthopanax senticosus, gynostemma pentaphyllum, turmeric, safflower, ganoderma lucidum and kudzu root in water for 1 hour; Step S2: adding water and boiling for 2 times, adding 10 times the amount of water for the first time, soaking for 30 minutes, boiling, keeping slightly boiling for 45 minutes, filtering through double-layer gauze to obtain a filtrate; adding 8 times the amount of water to the filter residue and boiling, keeping slightly boiling for 30 minutes, filtering through double-layer gauze to obtain a filtrate, combining the two filtrates, concentrating by rotary evaporation, and adding auxiliary materials to prepare one of oral liquid, tablets, capsules, and granules.

4. Use of the Chinese medicinal compound composition for treating coronary heart disease according to claim 1 in preparing medicine for treating coronary heart disease.

5. Use of the Chinese medicinal compound composition for treating coronary heart disease according to claim 1 in preparing a drug for treating coronary heart disease, characterized in that: Coronary heart disease belongs to the type of deficiency of both Qi and Yin, and accumulation of blood stasis and heat.

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