Fluorescent probe for detecting phenylethylamine in aqueous solution and urine and detection method thereof

Abstract

The invention belongs to the technical field of analytical chemistry, and particularly relates to a fluorescent probe for detecting phenylethylamine in an aqueous solution and urine and a detection method of the fluorescent probe, and the fluorescent probe for detecting phenylethylamine in the aqueous solution and urine is prepared from a cucurbit [8] uril aqueous solution and a coumarin derivative PDT aqueous solution; the coumarin derivative PDT is C20H21N2O3, the cucurbit [8] uril is C48H48N32O16, and the coumarin derivative PDT is C20H21N2O3. The fluorescent probe has remarkable advantages in the aspects of sensitivity, anti-interference performance, selectivity, quantitative detection capacity, low cost, simple and convenient preparation and the like.

Description

Technical Field

[0001] The present invention belongs to the technical field of analytical chemistry, and specifically relates to a fluorescent probe for detecting phenylethylamine in aqueous solution and urine and a detection method thereof. Background Art

[0002] Phenethylamine (PEA) is a naturally occurring biogenic amine that is called the "mood hormone" by scientists because of its significant effect on mood. When the human body ingests large amounts of PEA, it can induce the production of norepinephrine and dopamine, which can trigger feelings of excitement and hallucinations. Due to its potential to improve athletic performance, PEA has been listed as a banned substance by the international sports community. The detection of PEA in an athlete's urine may result in severe sanctions, such as disqualification from domestic and international competitions. In the past few decades, scientists have developed a variety of analytical methods for the detection of PEA, mainly including the following: 1. Electrochemical method: Detection using the redox properties of PEA has high sensitivity, but requires complex sample pretreatment processes and expensive instrumentation. 2. Ion chromatography: Determination by separating and detecting ionized PEA has good selectivity, but the operation is complex and time-consuming. 3. Atomic absorption spectroscopy: Detection using the interaction between PEA and metal ions has high sensitivity, but requires large experimental equipment and professional operators. These limitations make it difficult for existing traditional methods to be widely promoted in practical applications, especially in situations where fast and simple testing is required, such as doping testing in sports events.

[0003] Compared with the above-mentioned traditional methods, the fluorescence measurement method has attracted much attention because of its following advantages: 1. High sensitivity: it can detect extremely low concentrations of PEA. 2. Low detection cost: compared with large-scale instruments and equipment, the equipment cost required for fluorescence measurement is low. 3. Simple sample processing: no complicated sample pretreatment process is required. 4. Convenient operation: the operation steps are simple and easy to master. 5. Fast determination: the detection can be completed in a short time. 6. Real-time detection: the concentration change of PEA can be monitored in real time. For example, the patent with publication number CN116640132A discloses a coumarin fluorescent probe based on benzoxazole derivatives and its application. 7-Hydroxy-4-methylcoumarin-3-acetic acid and 6-bromobenzo[d]oxazole are dissolved in dimethyl sulfoxide solution, and the reaction solution is heated to reflux at 70-90°C for 3-4 hours under alkaline conditions of potassium carbonate. The obtained crude product is separated and purified by thin layer chromatography to obtain a coumarin fluorescent probe SYL-4 based on benzoxazole derivatives. The coumarin fluorescent probe obtained by the present invention can quickly identify biogenic amines (BAs) in a cetyltrimethylammonium bromide (CTAB) surfactant aqueous solution, but the reaction temperature of this method is relatively high and room temperature reaction cannot be achieved.

[0004] Although the patent with publication number CN117625178A discloses a method for preparing and using a supramolecular fluorescent probe for detecting aliphatic biogenic amines in water, the supramolecular fluorescent probe is constructed with symmetrical tetramethyl hexacyclic cucurbitacin (TMeQ[6]) as the host and 2-(2-pyridyl)benzimidazole as the guest, and recognizes aliphatic biogenic amines. The present invention uses ultraviolet-visible spectroscopy, fluorescence spectroscopy and nuclear magnetic titration to prove that TMeQ[6] and 2-(2-pyridyl)benzimidazole can form a host-guest supramolecular fluorescent probe with a molar ratio of 1:1, and has specific recognition for aliphatic biogenic amines (putrescine, cadaverine, spermine and spermidine) under aqueous medium conditions, and can be applied to the detection of biogenic amines in aqueous solution.

[0005] However, in the existing technology, there are few technologies that use eight-membered cucurbitacin as the main body and coumarin derivatives as the objects to construct a supramolecular system and detect phenylethylamine in aqueous solution or urine. Therefore, it is particularly important to develop a highly sensitive fluorescent probe that can not only meet the needs of rapid and simple detection, but also provide reliable results in practical applications, especially in doping detection in sports events and clinical diagnosis.

[0006] In summary, developing a fluorescence detection technology with low detection cost, low reaction cost, rapid and effective, selective and sensitive, especially a technology based on fluorescence measurement method, is an important issue that needs to be solved urgently. Summary of the invention

[0007] In view of the deficiencies of the prior art, the present invention provides a fluorescent probe for detecting phenylethylamine in aqueous solution and urine and a detection method thereof.

[0008] This is achieved specifically through the following technical solutions:

[0009] A fluorescent probe for detecting phenylethylamine in aqueous solution and urine, wherein the fluorescent probe for detecting phenylethylamine in aqueous solution and urine is prepared from an eight-membered cucurbitacin aqueous solution and a coumarin derivative PDT aqueous solution; the coumarin derivative PDT is C 20 H 21 N2O3.

[0010] The eight-membered melon ring: C 48 H 48 N 32 O 16 .

[0011] The probe molecular formula is (C 48 H 48 N 32 O 16 )2@C 20 H21 N2O3, the structure is as follows:

[0012]

[0013] The preparation method of the fluorescent probe for detecting phenylethylamine in aqueous solution and urine is as follows:

[0014] 1) Take the eight-membered cucurbit ring and dissolve it in water to obtain a concentration of 1×10 -4 mol / L solution A,;

[0015] 2) Take the coumarin derivative PDT and dissolve it in water to obtain a concentration of 1×10 -3 mol / L solution B;

[0016] 3) Solution A and solution B are mixed, and the mixed solution is reacted at room temperature to prepare a fluorescent probe for detecting phenylethylamine in aqueous solution and urine.

[0017] The molar ratio of the eight-membered cucurbitacin ring to the coumarin derivative PDT in the mixed solution is 2:1.

[0018] A method for detecting phenylethylamine in aqueous solution or urine using the above fluorescent probe is as follows:

[0019] 1) Take a fluorescent probe for detecting phenylethylamine in aqueous solution and urine, dilute it with secondary water at pH 7 to obtain a probe standard solution;

[0020] 2) adding the sample to be tested to the probe solution and mixing evenly to obtain a fluorescent probe solution to be tested, performing fluorescence emission at a fixed excitation wavelength of 312 nm, and determining whether the fluorescent probe solution to be tested contains phenylethylamine by observing the fluorescence intensity at 395 nm;

[0021] 3) preparing a phenylethylamine (PEA) standard solution with a concentration gradient, then adding the phenylethylamine standard solution with a concentration gradient to the fluorescent probe and mixing evenly to obtain a series of standard solutions, and then performing fluorescence emission spectrum measurement at a fixed excitation wavelength of 312 nm, drawing a standard curve of fluorescence intensity change at an emission wavelength of 395 nm, and calculating the fluorescence emission spectrum intensity change value ΔI at 395 nm before and after adding the sample to be tested according to the standard curve, so as to quantitatively detect phenylethylamine in the sample to be tested.

[0022] The concentration of the probe standard solution is 2×10 -5 mol / L.

[0023] In the step 3), when the fluorescence emission spectrum intensity at 395 nm of the fluorescent probe solution to be tested is enhanced, it indicates that phenylethylamine is contained in the sample to be tested.

[0024] The standard curve uses the concentration of phenethylamine as the abscissa and the difference between the fluorescence emission intensity of the probe at 395 nm and the fluorescence emission intensity at 395 nm after adding a phenethylamine standard solution with a concentration gradient is used as the ordinate.

[0025] The sample to be tested is an aqueous solution or urine containing phenylethylamine.

[0026] Beneficial effects:

[0027] 1. High sensitivity and anti-interference.

[0028] The fluorescent probe prepared by the present invention exhibits excellent performance in detecting phenylethylamine (PEA) in aqueous solution or urine. It not only has high sensitivity and can effectively detect PEA in a low concentration range, but also has strong anti-interference properties. In a complex urine simulation environment, the probe can still maintain high sensitivity and good anti-interference properties, ensuring the accuracy and reliability of the test results.

[0029] 2. The advantage of strong selectivity.

[0030] During the experiment, the fluorescent probe of the present invention only showed a significant response to phenylethylamine, and had almost no response to other interfering substances involved in the experiment. This property shows that the probe has extremely strong selectivity and can selectively identify and detect phenylethylamine in a complex environment, avoiding interference from other substances, thereby improving the accuracy and reliability of the detection.

[0031] 3. Quantitative detection capability.

[0032] According to the difference in fluorescence intensity change, the fluorescent probe of the present invention can realize the quantitative detection of phenylethylamine in aqueous solution or urine. Through experimental data, a linear relationship between the fluorescence intensity change value and the phenylethylamine concentration was established, and the linear regression equations were:

[0033] In aqueous solution, the linear range is 0-1×10 -4 M, y = 16.37x + 11.46, the correlation coefficient is 0.9749, x represents the concentration of phenylethylamine mol / L, and the detection limit is 1.16×10 -6 M;

[0034] In aqueous solution, the linear range is 1-4×10 -4 M, y = 5.076x-38.21, the correlation coefficient is 0.9987, x represents the concentration of phenylethylamine mol / L, and the detection limit is 3.73×10 -6 M;

[0035] In urine, the linear range is 0-1×10 -4M, y = 17.918x + 8.89, the correlation coefficient is 0.9746, x represents the concentration of phenylethylamine mol / L, and the detection limit is 1.05×10 -6 M;

[0036] In urine, the linear range is 1-4×10 -4 When M, y = 5.287x + 169.96, the correlation coefficient is 0.9921x represents the concentration of phenylethylamine mol / L, and the detection limit is 3.58×10 -6 M.

[0037] These linear relationships indicate that there is a good linear relationship between the change in fluorescence intensity and the concentration of phenylethylamine, and that the results can be used for the quantitative detection of phenylethylamine.

[0038] 4. Low cost and easy preparation.

[0039] The fluorescent probe of the present invention has the characteristics of low cost and simple preparation, which makes it have great application advantages in the field of biogenic amine recognition. Compared with the traditional complex and expensive detection method, the probe of the present invention can achieve efficient detection of phenylethylamine with lower cost and simpler preparation process, thereby reducing the detection cost and improving the detection efficiency.

[0040] 5. Expand the application areas.

[0041] The fluorescent probe of the present invention is based on the cucurbitacin-based supramolecular assembly, and its excellent performance is not limited to the detection of phenylethylamine, but also provides new ideas and possibilities for the application of the cucurbitacin-based supramolecular assembly in various fields. Its advantages in the recognition of biogenic amines can be extended to the detection and analysis of other biomolecules, providing new tools and methods for the fields of biomedicine, environmental monitoring, etc.

[0042] In summary, the fluorescent probe of the present invention exhibits significant advantages in terms of sensitivity, anti-interference, selectivity, quantitative detection capability, low cost and simple preparation. BRIEF DESCRIPTION OF THE DRAWINGS

[0043] Figure 1 The fluorescence emission spectra and ultraviolet absorption spectra of the eight-membered cucurbit ring and coumarin derivative PDT. Among them: a is a series of solutions prepared by the molar ratio method, and the fluorescence emission spectra obtained by data processing using the origin software; c is a series of solutions prepared by the molar ratio method, and the ultraviolet absorption spectra obtained by data processing using the origin software; b is the fluorescence emission spectrum of PDT at 395nm with N Q[8] / N PDT The relationship diagram of the change is the point diagram obtained by data processing using origin software; d is the ultraviolet absorption spectrum of PDT at 295nm with N Q[8] / NPDT The relationship diagram of the changes, the point diagram obtained by data processing using origin software;

[0044] Figure 2 This is a diagram of the PDT inclusion pattern of eight-membered cucurbitacin and coumarin derivatives;

[0045] Figure 3 The results of PDT nuclear magnetic titration of eight-membered cucurbitacin and coumarin derivatives;

[0046] Figure 4 This is a photo of the fluorescence effect of the fluorescent probe interacting with more than a dozen biogenic amines;

[0047] Figure 5 The fluorescence diagram for the specific selection of fluorescent probes for biogenic amines in aqueous solution and the relative error diagram for the anti-interference of PEA for the specific selection of fluorescent probes for other biogenic amines as interfering substances;

[0048] in, Figure 4 and Figure 5 Where 1-15 are tryptamine, N,n-dimethylethylenediamine, ammonia, ethylamine, n-propylamine, 1,2-propylenediamine, trimethylamine, dimethylamine, putrescine, cadaverine, glutathione, hydroxylamine hydrochloride, tyramine, hydrazine hydrate, and triethylamine, respectively;

[0049] Figure 6 The fluorescence titration spectrum and titration detection limit of adding concentration gradient PEA standard solution to the probe standard solution in aqueous solution;

[0050] Figure 7 The relative error diagram of the anti-interference of PEA selected specifically for the probe when the main components in urine are used as interfering substances and the fluorescence titration spectrum curve of the PEA standard solution with a concentration gradient added; a is the fluorescence emission spectrum comparison of several main components in urine as interfering substances for the probe specific selection of PEA; b is the statistics of the relative error of the anti-interference of several main components in urine as interfering substances for the probe specific selection of PEA; c is the fluorescence titration spectrum comparison of the probe standard urine with the PEA standard solution with a concentration gradient added; d is the point diagram obtained by processing the fluorescence titration spectrum data of the probe standard urine with the PEA standard solution with a concentration gradient added;

[0051] Figure 8 The titration detection limit diagram of adding concentration gradient PEA standard solution to the probe standard urine. DETAILED DESCRIPTION

[0052] The specific embodiments of the present invention are further described in detail below, but the present invention is not limited to these embodiments, and any improvement or substitution based on the basic spirit of the present embodiment still falls within the scope of protection required by the claims of the present invention.

[0053] Example 1

[0054] 1. Preparation of fluorescent probe standard solution:

[0055] (1) Accurately weigh an appropriate amount of eight-membered cucurbit ring, dissolve it in secondary water and dilute it to a 100 mL volumetric flask to obtain a concentration of 1.0 × 10 -4 mol / L eight-membered cucurbitacin solution.

[0056] (2) Accurately weigh an appropriate amount of PDT, dissolve it in secondary water and dilute it to a 100 mL volumetric flask to obtain a concentration of 1.0 × 10 -3 mol / L PDT solution.

[0057] (3) Mix the two solutions in a volume ratio of 20:1 and react at room temperature for a period of time to obtain a concentration of 2.0×10 - 5 mol / L fluorescent probe standard solution;

[0058] The combination of fluorescent probes such as Figure 1 As shown, 1 H NMR spectrum Figure 2 As shown, the structural formula is as follows:

[0059]

[0060] 2. Preparation of PEA standard solution:

[0061] Accurately weigh an appropriate amount of phenylethylamine, dissolve it in secondary water at pH = 7 and make up to 10 mL to obtain a concentration of 1.0 × 10 - 2 mol / L PEA standard solution;

[0062] 3. Preparation of interfering substance solution:

[0063] 3.1 Accurately weigh analytically pure standard products of tryptamine, N,n-dimethylethylenediamine, ammonia, ethylamine, n-propylamine, 1,2-propylenediamine, trimethylamine, dimethylamine, putrescine, cadaverine, glutathione, hydroxylamine hydrochloride, tyramine, hydrazine hydrate, and triethylamine, and dissolve them in a secondary aqueous solution at pH = 7 to obtain a concentration of 1.0×10 -2 mol / L standard solutions of various interfering biogenic amines;

[0064] 3.2 Accurately weigh analytically pure standard products of MgCl2, KCl, NaCl, CaCl2, creatinine (Crea), glucose (Glu), Na2SO4, NH4Cl, NaHCO3, serum creatinine (Cre), urea (Urea), uric acid, and L-proline (L-Pre), and dissolve them in a secondary aqueous solution at pH = 7 to obtain a concentration of 1.0×10 -2mol / L standard solutions of various urine interfering components;

[0065] 4. PEA spectrum drawing by fluorescent probe specificity selection:

[0066] Twenty times the equivalent of various interfering biogenic amine standard solutions were added to the fluorescent probe standard solution, and a series of fluorescence curves were measured at an excitation wavelength of 312 nm. The fluorescence effect diagram is shown in FIG. Figure 4 , Figure 5 (a), wherein 1-15 are tryptamine, N,n-dimethylethylenediamine, ammonia, ethylamine, n-propylamine, 1,2-propylenediamine, trimethylamine, dimethylamine, putrescine, cadaverine, glutathione, hydroxylamine hydrochloride, tyramine, hydrazine hydrate, triethylamine;

[0067] 5. Drawing of the anti-interference spectrum of PEA by fluorescent probe specific recognition:

[0068] Twenty times the equivalent of various interfering biogenic amine standard solutions and twenty times the equivalent of PEA standard solution were added to the fluorescent probe standard solution, and a series of fluorescence curves were measured at an excitation wavelength of 312 nm. The fluorescence effect diagram is shown in FIG. Figure 5 (b), wherein 1-15 are tryptamine, N,n-dimethylethylenediamine, ammonia, ethylamine, n-propylamine, 1,2-propylenediamine, trimethylamine, dimethylamine, putrescine, cadaverine, glutathione, hydroxylamine hydrochloride, tyramine, hydrazine hydrate, and triethylamine, respectively;

[0069] 6. Fluorescence spectrum and standard curve drawing of PEA titration fluorescent probe in water:

[0070] Take a quartz fluorescence cuvette and add 2.0×10 -5 mol / L fluorescent probe standard solution 3000μL, accurately add 1.0×10 -2 mol / LPEA standard solution 6 μL was placed in a cuvette, stirred evenly, and the fluorescence emission spectrum was determined at a fixed excitation wavelength of 312 nm;

[0071] According to the above operation, a quantitative amount of 6 μL PEA standard solution is continuously added to the above 3000 μL probe solution, and a series of fluorescence curves are measured at an excitation wavelength of 312 nm until the vertical coordinate value of the fluorescence curve changes slowly, and the titration operation can be stopped;

[0072] Then, the PEA concentration is used as the horizontal axis, and the difference between the fluorescence emission intensity of the probe at 395 nm and the fluorescence emission intensity of adding different concentrations of PEA is used as the vertical axis to obtain a standard curve, such as Figure 6 shown).

[0073] 7. Anti-interference experiment in urine and drawing of standard curve: Same as step 5 and step 6, only need to replace various interfering biogenic amine standard solutions with various urine interfering component standard solutions. The anti-interference fluorescence spectrum, titration spectrum and standard curve drawing in urine are as follows: Figure 7 , Figure 8 shown.

[0074] 8. Sample testing:

[0075] Take the solution containing PEA, add the prepared fluorescent probe standard solution into it, and control its concentration not to exceed the linear range of (0-40)×10 -5 mol / L, and under the excitation wavelength of 312nm, observe whether there is an enhancement in fluorescence intensity at 395nm. If so, it means that it contains PEA; if not, it means that it does not contain PEA.

[0076] Example 2

[0077] A method for preparing a fluorescent probe for detecting phenylethylamine in aqueous solution and urine comprises the steps of:

[0078] (1) Accurately weigh an appropriate amount of eight-membered cucurbit ring, dissolve it in secondary water and dilute it to a 100 mL volumetric flask to obtain a concentration of 1.0 × 10 -4 mol / L eight-membered cucurbit ring solution;

[0079] (2) Accurately weigh an appropriate amount of the coumarin derivative PDT, dissolve it in secondary water and dilute it to a 100 mL volumetric flask to obtain a concentration of 1.0 × 10 -3 mol / L coumarin derivative PDT solution;

[0080] (3) 1.0×10 -4 mol / L eight-membered cucurbitacin solution and 1.0×10 -3 mol / L coumarin derivative PDT solution was mixed at a volume ratio of 20:1 and reacted at room temperature for a period of time to obtain a concentration of 2.0×10 -5 mol / L fluorescent probe standard solution.

[0081] Example 3

[0082] A method for preparing a fluorescent probe for detecting phenylethylamine in aqueous solution and urine comprises the steps of:

[0083] (1) Accurately weigh an appropriate amount of eight-membered cucurbitacin ring, dissolve it in secondary water and dilute it to a 500 mL volumetric flask to obtain a concentration of 1×10 -4 mol / L eight-membered cucurbit ring solution;

[0084] (2) Accurately weigh an appropriate amount of the coumarin derivative PDT, dissolve it in secondary water and dilute it to 100 mL in a volumetric flask to obtain a concentration of 1×10 -4 mol / L coumarin derivative PDT solution;

[0085] (3) 1.0×10 -4 mol / L eight-membered cucurbitacin solution and 1.0×10 -4 mol / L coumarin derivative PDT solution was mixed at a volume ratio of 2:1 and reacted at room temperature for a period of time to obtain a concentration of 2.0×10 -5 mol / L fluorescent probe standard solution.

[0086] Example 4

[0087] The specific operations of each determination in the detection method in Example 1 are:

[0088] (1) Determination of standard curve:

[0089] Take 5 5 mL centrifuge tubes and add 2.0 × 10 -5 mol / L fluorescent probe solution 3000 μL, and then 1.0×10 -2 6 μL, 9.0 μL, 12.0 μL, 15.0 μL, 18.0 μL, 21.0 μL, ..., 36.0 μL of 1 mol / L PEA standard solution were made up to volume with a secondary aqueous solution of pH=7 and shaken evenly. After standing at room temperature for 10-20 min, the fluorescence emission spectrum was determined at a fixed excitation wavelength of 312 nm. The standard curve was drawn with the PEA concentration as the abscissa and the difference (ΔI) between the probe fluorescence emission intensity (I0) at 395 nm and the fluorescence emission intensity (I) of the added concentration gradient PEA as the ordinate. The detection limit of PEA by the fluorescent probe was calculated based on the slope of the standard curve and the standard deviation of 10 blank values.

[0090] (3) Sample testing:

[0091] Take an aqueous solution containing PEA but with unknown concentration and add it to the prepared fluorescent probe standard solution, and control its concentration not to exceed the linear range of (0-40.0)×10 -5 mol / L, and under the excitation wavelength of 312nm, the fluorescence intensity is enhanced at 395nm, which means that the water sample contains PEA.

[0092] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical solutions and inventive concepts of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A fluorescent probe for detecting phenylethylamine in aqueous solution and urine, characterized in that: The fluorescent probe for detecting phenylethylamine in aqueous solution and urine is prepared from an aqueous solution of eight-membered cucurbitacin and an aqueous solution of coumarin derivative PDT; the coumarin derivative PDT is C 20 H 21 N2O3.

2. A fluorescent probe for detecting phenylethylamine in aqueous solution and urine according to claim 1, characterized in that: The eight-membered melon ring: C 48 H 48 N 32 O 16 .

3. A fluorescent probe for detecting phenylethylamine in aqueous solution and urine according to claim 1, characterized in that: The probe molecular formula is (C 48 H 48 N 32 O 16 )2@C 20 H 21 N2O3, the structure is as follows:

4. The fluorescent probe for detecting phenylethylamine in aqueous solution and urine according to claim 1, characterized in that: The preparation method of the fluorescent probe for detecting phenylethylamine in aqueous solution and urine is as follows: 1) Take the eight-membered cucurbit ring and dissolve it in water to obtain a concentration of 1×10 -4 mol / L solution A,; 2) Take the coumarin derivative PDT and dissolve it in water to obtain a concentration of 1×10 -3 mol / L solution B; 3) Solution A and solution B are mixed, and the mixed solution is reacted at room temperature to prepare a fluorescent probe for detecting phenylethylamine in aqueous solution and urine.

5. The fluorescent probe for detecting phenylethylamine in aqueous solution and urine according to claim 3, characterized in that: The molar ratio of the eight-membered cucurbitacin ring to the coumarin derivative PDT in the mixed solution is 2:

1.

6. A method for detecting phenylethylamine in aqueous solution or urine using the fluorescent probe according to any one of claims 1 to 5, the method being as follows: 1) Take a fluorescent probe for detecting phenylethylamine in aqueous solution and urine, dilute it with secondary water at pH 7 to obtain a probe standard solution; 2) adding the sample to be tested to the probe solution and mixing evenly to obtain a fluorescent probe solution to be tested, performing fluorescence emission at a fixed excitation wavelength of 312 nm, and determining whether the fluorescent probe solution to be tested contains phenylethylamine by observing the fluorescence intensity at 395 nm; 3) preparing a phenethylamine standard solution with a concentration gradient, then adding the phenethylamine standard solution with a concentration gradient to the fluorescent probe and mixing evenly to obtain a series of standard solutions, then performing fluorescence emission spectrum measurement at a fixed excitation wavelength of 312 nm, plotting a standard curve of fluorescence intensity change at an emission wavelength of 395 nm, and calculating the fluorescence emission spectrum intensity change value ΔI at 395 nm before and after adding the sample to be tested according to the standard curve, so as to quantitatively detect the phenethylamine in the sample to be tested.

7. The method for detecting phenylethylamine in aqueous solution or urine using a fluorescent probe according to claim 5, characterized in that: The concentration of the probe standard solution is 2×10 -5 mol / L.

8. The method for detecting phenylethylamine in aqueous solution or urine using a fluorescent probe according to claim 5, characterized in that: In the step 3), when the fluorescence emission spectrum intensity at 395 nm of the fluorescent probe solution to be tested is enhanced, it indicates that phenylethylamine is contained in the sample to be tested.

9. The method for detecting phenylethylamine in aqueous solution or urine using a fluorescent probe according to claim 5, characterized in that: The standard curve uses the concentration of phenethylamine as the abscissa and the difference between the fluorescence emission intensity of the probe at 395 nm and the fluorescence emission intensity at 395 nm after adding a phenethylamine standard solution with a concentration gradient is used as the ordinate.

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