Thin-layer chromatography detection method for cistanche in cistanche and herba epimedii black tea
Through the thin-layer chromatography detection method, using appropriate expanding agents and ultraviolet lamp color development treatment, the problem of large deviations and low accuracy of the detection of Cistanche ingredients in Cistanche Epimedium Black Tea was solved, and the detection effect of high accuracy was achieved.
Patent Information
- Application Number
- CN202510124384.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-01-26
- Publication Date
- 2025-05-06
AI Technical Summary
The prior art has problems of large deviations and low accuracy when detecting the Cistanche ingredients in Cistanche Epimedium Black Tea.
A thin layer chromatography detection method is used to prepare test sample solution and reference solution, and chromatographic analysis is performed using appropriate expanders (such as different ratios of ethanol, formic acid and water). After the color development treatment, the spot position and color are observed under the ultraviolet lamp to determine the component content.
The high accuracy detection of the Cistanche marking components of Cistanche and Mullin in Cistanche Epimedium Black Tea was achieved, and the problems of poor identification effect and prone to chromatography in the existing methods were overcome, and the accuracy and comprehensiveness of quality control were improved.
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Abstract
Description
Technical Field
[0001] The present application relates to the technical field of food detection, and in particular to a thin layer chromatography detection method for Cistanche deserticola in Cistanche epimedium black tea. Background Art
[0002] Cistanche and Epimedium Black Tea is a health food that relieves physical fatigue, made from Cistanche, jujube, epimedium, Acanthopanax, black tea, dextrin and other main raw and auxiliary materials, using Chinese medicine water extraction technology combined with modern technology. Cistanche is one of the important ingredients in this product, and accurate testing of it can ensure the quality and efficacy of the product.
[0003] Cistanche tubulosa (Schenk) Wight, CtW, is a perennial parasitic herb belonging to the genus Cistanche in the family Orobanchaceae. It is also known as Cistanche and Dayun. The chemical components of Cistanche tubulosa mainly include glycosides, terpenes, alkaloids, sugars, trace elements and amino acids. The ethanol extract of Cistanche tubulosa can delay the occurrence of exercise fatigue in mice, and has the effects of resisting exercise fatigue in mice, improving exercise endurance and delaying the occurrence of exhaustion. Total oligosaccharides of Cistanche tubulosa improve central and exercise-induced fatigue by regulating neurotransmitter levels, enhancing glycogen storage and improving antioxidant enzyme activity, indicating its potential therapeutic benefits in fatigue-related diseases. The quality control of Cistanche tubulosa medicinal materials is controlled according to the thin layer chromatography method (General Rule 0502) in the "Chinese Pharmacopoeia 2020 Edition Volume 1" to control echinacoside and verbascoside.
[0004] However, there are currently problems with large deviations and low accuracy when testing the Cistanche deserticola component in Cistanche epimedium black tea. Summary of the invention
[0005] Based on this, the present application provides a thin layer chromatography detection method for Cistanche deserticola in Cistanche epimedium black tea with good separation effect and high detection accuracy.
[0006] The specific technical solutions are as follows:
[0007] A thin layer chromatography detection method for Cistanche deserticola in Cistanche epimedium black tea comprises the following steps:
[0008] Prepare the test solution of Cistanche deserticola and Epimedium black tea, echinacea glycoside reference substance, verbascoside reference substance and Cistanche deserticola reference medicinal material solution;
[0009] The test solution of the Cistanche epimedium black tea to be tested, the echinacoside reference substance, the verbascoside reference substance and the Cistanche deserticola reference medicinal material solution are spotted on the same polyamide thin layer plate, developed with a developing agent, taken out, dried and then subjected to color development treatment;
[0010] Wherein, the developing agent is selected from the following:
[0011] (1) ethanol, formic acid and water in a volume ratio of 2:(0.3-0.8):(6-10);
[0012] (2) ethanol, acetic acid and water in a volume ratio of 2:(0.8-1.2):(6-10);
[0013] (3) Methanol, acetic acid and water in a volume ratio of 1:(1.5-2.5):(6-10).
[0014] In some of the embodiments, the developing agent is ethanol, formic acid and water in a volume ratio of 2:(0.3-0.8):(6-10).
[0015] In some of these embodiments, the color development process includes the step of observing under ultraviolet light.
[0016] In some embodiments, the wavelength of the ultraviolet lamp is 365nm.
[0017] In some embodiments, the test solution of Cistanche deserticola and Epimedium black tea comprises the following steps:
[0018] Adding a first solvent to the tested Cistanche deserticola and Epimedium black tea for extraction, evaporating the obtained extract to dryness to prepare a first solid;
[0019] The first solid is added into the second solvent to be dissolved, so as to prepare the test solution of the Cistanche deserticola and Epimedium black tea to be tested;
[0020] And / or, preparing the Cistanche deserticola control medicinal material solution comprises the following steps:
[0021] Adding a first solvent to a Cistanche deserticola reference medicinal material for extraction, and evaporating the obtained extract to dryness to prepare a first solid;
[0022] The first solid is added into the second solvent to dissolve, so as to prepare the Cistanche deserticola control medicinal material solution.
[0023] In some embodiments, the first solvent is each independently methanol.
[0024] In some embodiments, the second solvent is each independently methanol.
[0025] In some embodiments, adding the first solvent for extraction includes the step of performing ultrasonic extraction; the conditions of the ultrasonic extraction include: a frequency of 30kHZ to 50kHZ, and a power of 250W to 350W.
[0026] In some embodiments, the ultrasonic extraction time is 5 min to 25 min.
[0027] In some embodiments, the temperature for evaporating the extract to dryness is 40°C to 50°C.
[0028] The above-mentioned thin layer chromatography detection method of Cistanche deserticola in Cistanche epimedium black tea adopts suitable developing agent and thin layer plate for detection, which can make the adsorption of Cistanche deserticola marker components echinacoside and verbascoside in Cistanche epimedium black tea more stable and uniform, and the chromatographic migration of test sample and reference sample is stable, and the chromatographic position matching is more accurate, thereby overcoming the problems of poor identification effect and easy deviation of chromatography in existing methods, and realizing high-accuracy detection of Cistanche deserticola in Cistanche epimedium black tea.
[0029] At the same time, the thin layer chromatography detection method also has the advantages of high sensitivity, simple detection operation, short detection time, low cost, and clear and interference-free corresponding thin layer chromatography spots, which improves the accuracy and comprehensiveness of the quality control of Cistanche and Epimedium black tea, and is conducive to the establishment and overall evaluation of the quality standard of Cistanche and Epimedium black tea. BRIEF DESCRIPTION OF THE DRAWINGS
[0030] Figure 1 This is a thin layer plate image of comparative example 1 using methanol: acetic acid: water (2:1:7) as the developing agent and a polyamide plate as the thin layer plate.
[0031] Figure 2 This is a thin layer plate image of Example 1 using ethanol:formic acid:water (2:0.5:8) as the developing agent and a polyamide plate as the thin layer plate.
[0032] Figure 3 This is a thin layer plate image of comparative example 2 using methanol:formic acid:water (2:1:7) as the developing agent and a polyamide plate as the thin layer plate.
[0033] Figure 4 This is a thin layer plate image of Example 2 using ethanol: acetic acid: water (2:1:7) as the developing agent and a polyamide plate as the thin layer plate.
[0034] Figure 5 This is a thin layer plate image of Example 3 using methanol: acetic acid: water (1:2:7) as the developing agent and a polyamide plate as the thin layer plate.
[0035] Figure 6 This is a thin layer plate image of comparative example 3 using methanol: acetic acid: water (2:5:3) as the developing agent and a polyamide plate as the thin layer plate.
[0036] Figure 7 This is a thin layer plate image of comparative example 4 using methanol: acetic acid: water (4:1:5) as the developing agent and a polyamide plate as the thin layer plate.
[0037] Figure 8This is a thin layer plate image of comparative example 5 using methanol: acetic acid: water (7:1:2) as the developing agent and a polyamide plate as the thin layer plate.
[0038] Fig. 9 This is a thin layer plate image of comparative example 6 using chloroform: methanol: water (7:2.5:0.25) as the developing agent and a polyamide plate as the thin layer plate.
[0039] Fig.10 This is the thin layer plate image of comparative example 7 using ethanol: water (2:8) as the developing agent and a polyamide plate as the thin layer plate.
[0040] Fig.11 This is a thin layer plate image of comparative example 8 using acetic acid: water (2:8) as the developing agent and a polyamide plate as the thin layer plate.
[0041] Fig.12 This is a thin layer plate image of comparative example 9 using acetic acid: water (3:6) as the developing agent and a polyamide plate as the thin layer plate.
[0042] Fig.13 This is a thin layer plate image of comparative example 10 using ethanol: acetic acid: water (6:2:2) as the developing agent and a polyamide plate as the thin layer plate.
[0043] Fig.14 This is the thin layer plate image of comparative example 11 using ethyl acetate:methanol (8:1) as the developing solvent and a polyamide plate as the thin layer plate.
[0044] Fig.15 This is the thin layer plate image of comparative example 12 with ethyl acetate:formic acid:water (11:2:1.5) as the developing agent and polyamide plate as the thin layer plate.
[0045] Fig.16 This is the thin layer plate image of comparative example 13 with ethyl acetate:formic acid:water (11:2:1.5) as the developing solvent and silica gel G plate as the thin layer plate.
[0046] Fig.17 This is the thin layer plate image of comparative example 14 with n-butanol: acetic acid: water (5:4:1) as the developing agent and silica gel GF254 plate as the thin layer plate.
[0047] Fig.18 This is a thin layer plate image of comparative example 15 using methanol: acetic acid: water (2:1:7) as the developing solvent and silica gel H plate as the thin layer plate.
[0048] Fig.19 This is the thin layer plate image of Comparative Example 16 using methanol: acetic acid: water (2:1:7) as the developing solvent and silica gel G plate as the thin layer plate.
[0049] Fig. 20This is the thin layer plate image of comparative example 17 with methanol: acetic acid: water (2:1:7) as the developing agent and silica gel GF254 plate as the thin layer plate.
[0050] Among them, the samples spotted on each thin layer plate are, from left to right, Cistanche epimedium black tea test solution, Cistanche control medicinal material solution, echinacea glycoside reference substance, verbascoside reference substance, and negative control medicinal material. DETAILED DESCRIPTION
[0051] Below in conjunction with specific embodiment, the thin layer chromatography detection method of Cistanche deserticola in Cistanche epimedium black tea of the present application is further described in detail. The present application can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the disclosure of the present application more thoroughly understood.
[0052] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art to which this application belongs. The terms used herein in the specification of this application are only for the purpose of describing specific embodiments and are not intended to limit this application.
[0053] The optional scope of the terms "and / or", "or / and", and "and / or" used in this article includes any one of two or more related listed items, and also includes any and all combinations of the related listed items, said any and all combinations include any combination of any two related listed items, any more related listed items, or all related listed items.
[0054] As used herein, "one or more" refers to any one, any two, or any two or more of the listed items.
[0055] In the present application, the technical features described in an open manner include closed technical solutions composed of the listed features, and also include open technical solutions containing the listed features.
[0056] In this application, when it comes to numerical ranges, unless otherwise specified, the above numerical ranges are deemed to be continuous and include the minimum and maximum values of the range, as well as each value between such minimum and maximum values. Further, when a range refers to an integer, each integer between the minimum and maximum values of the range is included. In addition, when multiple ranges are provided to describe features or characteristics, the ranges can be merged. In other words, unless otherwise specified, all ranges disclosed herein should be understood to include any and all subranges included therein.
[0057] The percentage contents involved in this application, unless otherwise specified, refer to mass percentage for solid-liquid mixing and solid-solid mixing, and refer to volume percentage for liquid-liquid mixing.
[0058] The percentage concentrations mentioned in this application, unless otherwise specified, refer to the final concentration, which refers to the percentage of the added component in the system after the addition of the component.
[0059] The temperature parameters in this application, unless otherwise specified, allow for both constant temperature treatment and treatment within a certain temperature range. The constant temperature treatment allows the temperature to fluctuate within the accuracy range of instrument control.
[0060] The room temperature in the present application generally refers to 4°C to 30°C, preferably 20±5°C.
[0061] Some examples of the present application provide a thin layer chromatography detection method for Cistanche deserticola in Cistanche epimedium black tea, comprising the following steps:
[0062] Prepare the test solution of Cistanche deserticola and Epimedium black tea, echinacea glycoside reference substance, verbascoside reference substance and Cistanche deserticola reference medicinal material solution;
[0063] The test solution of the Cistanche epimedium black tea to be tested, the echinacoside reference substance, the verbascoside reference substance and the Cistanche deserticola reference medicinal material solution are spotted on the same polyamide thin layer plate, developed with a developing agent, taken out, dried and then subjected to color development treatment;
[0064] Wherein, the developing agent is selected from the following:
[0065] (1) ethanol, formic acid and water in a volume ratio of 2:(0.3-0.8):(6-10);
[0066] (2) ethanol, acetic acid and water in a volume ratio of 2:(0.8-1.2):(6-10);
[0067] (3) Methanol, acetic acid and water in a volume ratio of 1:(1.5-2.5):(6-10).
[0068] It can be understood that after the color development treatment, it is possible to judge whether the sample contains echinacoside and verbascoside components and preliminarily determine their content based on the spot positions, colors and other characteristics of the test sample and the reference substance on the thin layer plate.
[0069] Without limitation, the above-mentioned thin layer chromatography detection method further includes the steps of preparing a negative control medicinal material solution and spotting the negative control medicinal material solution on the same polyamide thin layer plate, so as to better reflect the accuracy of the target spot. It can be understood that the "negative control medicinal material" refers to the rest of the samples that are the same as the Cistanche Epimedium Black Tea, but do not contain the Cistanche medicinal material.
[0070] In some examples, the developing agent is ethanol, formic acid and water in a volume ratio of 2:(0.3-0.8):(6-10).
[0071] In some of these examples, the color development process includes a step of viewing under ultraviolet light.
[0072] In some examples, the UV lamp has a wavelength of 365 nm.
[0073] In addition, the accuracy and sensitivity of the detection can be further improved by effectively extracting the echinacoside and verbascoside components in the Cistanche deserticola and Epimedium black tea.
[0074] In some of these examples, the test solution of Cistanche deserticola and Epimedium black tea includes the following steps:
[0075] Adding a first solvent to the tested Cistanche deserticola and Epimedium black tea for extraction, evaporating the obtained extract to dryness to prepare a first solid;
[0076] The first solid is added into the second solvent to be dissolved, so as to prepare the test solution of the Cistanche deserticola and Epimedium black tea to be tested.
[0077] In some of the examples, preparing the Cistanche deserticola control medicinal material solution includes the following steps:
[0078] Adding a first solvent to a Cistanche deserticola reference medicinal material for extraction, and evaporating the obtained extract to dryness to prepare a first solid;
[0079] The first solid is added into the second solvent to dissolve, so as to prepare the Cistanche deserticola control medicinal material solution.
[0080] In some examples, the first solvent is each independently methanol.
[0081] In some examples, the second solvent is each independently methanol.
[0082] In some examples, adding the first solvent for extraction includes the step of performing ultrasonic extraction; the conditions of the ultrasonic extraction include: a frequency of 30kHZ to 50kHZ, and a power of 250W to 350W. Specifically, the frequency includes but is not limited to: 30kHZ, 35kHZ, 40kHZ, 45kHZ, 50kHZ, or a range between any two of the foregoing; the power includes but is not limited to: 250W, 280W, 300W, 320W, 350W, or a range between any two of the foregoing.
[0083] In some examples, the ultrasonic extraction time is 5 min to 25 min. Specifically, the ultrasonic extraction time includes but is not limited to: 5 min, 10 min, 15 min, 20 min, 25 min or a range between any two of the foregoing.
[0084] In some examples, the temperature of evaporating the obtained extract to dryness is 40° C. to 50° C. Specifically, the temperature of evaporating to dryness includes, but is not limited to, 40° C., 43° C., 45° C., 47° C., and 50° C. Without limitation, the method of evaporating the obtained extract to dryness can be vacuum rotary evaporation, with a vacuum degree of 0.09-0.095 MPa and a rotation speed of 60±2 rpm.
[0085] For experimental parameters not specified in the following specific embodiments, reference is made to the instructions given in the present application document, and reference may also be made to experimental manuals in the art or other experimental methods known in the art, or to experimental conditions recommended by manufacturers.
[0086] The raw materials and reagents involved in the following specific embodiments can be obtained from the market, or those skilled in the art can prepare them according to known means. The Cistanche Epimedium Black Tea used in the embodiment is from Xinjiang Huachun Pharmaceutical Co., Ltd., with the following proportions: Cistanche Tubulosa 1410g, jujube 990g, epimedium 990g, Acanthopanax Senticosus 990g, black tea 2010g, dextrin 99g, and this product is a 3.0g / bag bagged tea brewing agent.
[0087] Example 1
[0088] This embodiment is a thin layer chromatography detection method of Cistanche deserticola in Cistanche epimedium black tea.
[0089] (1) Preparation of reference substance stock solution
[0090] Take the echinacoside reference substance and the verbascoside reference substance, add methanol to prepare solutions containing 1 mg per 1 mL, respectively, as the echinacoside reference substance solution and the verbascoside reference substance solution.
[0091] (2) Preparation of control medicinal material solution
[0092] Take 1 g of Cistanche deserticola control medicinal material, add 20 mL of methanol, treat with ultrasound (frequency: 40 kHz, power: 300 W) for 15 minutes, filter, and concentrate the filtrate to near dryness using a vacuum rotary evaporator (water bath temperature 45°C, vacuum degree 0.09-0.095 MPa, speed 60±2 rpm), and add 2 mL of methanol to the residue to dissolve it as the control drug solution.
[0093] (3) Preparation of test solution
[0094] Take 1g of Cistanche deserticola and Epimedium black tea powder, add 20mL of methanol, treat with ultrasound (frequency: 40kHZ, power: 300W) for 15 minutes, filter, and concentrate the filtrate to near dryness using a vacuum rotary evaporator (water bath temperature 45°C, vacuum degree 0.09-0.095MPa, speed 60±2rpm), and add 2mL of methanol to the residue to dissolve it as the test solution.
[0095] (4) Negative control drugs
[0096] To prepare the negative control medicinal material of Cistanche deserticola and Epimedium black tea, the four medicinal materials of jujube, epimedium, Acanthopanax senticosus and black tea were mixed and crushed according to the compound ratio, and the negative control medicinal material solution was prepared in the same way as the test sample solution.
[0097] (5) Chromatographic conditions
[0098] According to the thin layer chromatography method (General Rule 0502), 2 μL of each of the five solutions mentioned above were taken and spotted on the same polyamide thin layer plate, and ethanol:formic acid:water (2:0.5:8, volume ratio) was used as the developing agent. The plate was developed, taken out, dried, and examined under ultraviolet light (365 min).
[0099] The thin layer chromatography detection method of Examples 2 to 3 and Comparative Examples 1 to 12 is the same as that of Example 1, the main difference being that different developing agents are used. The specific developing agents are shown in Table 1 below. The detection results of Examples 1 to 3 and Comparative Examples 1 to 12 are shown in Table 1 below. Figures 1 to 15 As shown:
[0100] Table 1
[0101] Developing agent Attached photos Comparative Example 1 Methanol: acetic acid: water (2:1:7) Figure 1 Example 1 Ethanol: Formic acid: Water (2:0.5:8) Figure 2 Comparative Example 2 Methanol:formic acid:water (2:1:7) Figure 3 Example 2 Ethanol: acetic acid: water (2:1:7) Figure 4 Example 3 Methanol: acetic acid: water (1:2:7) Figure 5 Comparative Example 3 Methanol: acetic acid: water (2:5:3) Figure 6 Comparative Example 4 Methanol: acetic acid: water (4:1:5) Figure 7 Comparative Example 5 Methanol: acetic acid: water (7:1:2) Figure 8 Comparative Example 6 Chloroform: methanol: water (7: 2.5: 0.25) Fig. 9 Comparative Example 7 Ethanol: Water (2:8) Fig.10 Comparative Example 8 Acetic acid: water (2:8) Fig.11 Comparative Example 9 Acetic acid: water (3:6) Fig.12 Comparative Example 10 Ethanol: acetic acid: water (6:2:2) Fig.13 Comparative Example 11 Ethyl acetate: methanol (8:1) Fig.14 Comparative Example 12 Ethyl acetate: Formic acid: Water (11:2:1.5) Fig.15
[0102] Combination Figures 1 to 15 It can be seen that the developing agents of Examples 1 to 3 can effectively separate the characteristic ingredients of Cistanche deserticola medicinal materials, echinacoside and verbascoside, in Cistanche epimedium black tea, and the spots are clear, the separation degree of impurity spots is high, the interference is small, and the detection results are accurate, among which Example 1 is the best. Comparative Examples 1 to 12 change the type of developing agent, and there are problems such as overlap with adjacent spots, unclear spots, and excessive development, which makes it difficult to detect Cistanche deserticola in Cistanche epimedium black tea.
[0103] The thin layer chromatography detection method of comparative example 13 is the same as that of comparative example 12, the main difference being that the thin layer plate used is a silica gel G plate. The detection results are as follows: Fig.16 shown.
[0104] The thin layer chromatography detection method of Comparative Example 14 is the same as that of Example 1, except that the developing solvent used is n-butanol: acetic acid: water (5:4:1), and the thin layer plate used is silica gel GF254 plate. The detection results are shown in FIG. Fig.17 shown.
[0105] The thin layer chromatography detection method of Comparative Examples 15 to 17 is the same as that of Comparative Example 1, the main difference being that different thin layer plates are used. The specific thin layer plates are shown in Table 2 below. The detection results are shown in Table 2 below. Figures 18 to 20 shown.
[0106] Table 2
[0107] Thin layer plate Attached photos Comparative Example 15 Silicone H plate Fig.18 Comparative Example 16 Silicone G plate Fig.19 Comparative Example 17 Silicone GF254 Fig. 20
[0108] Combination Figure 1 and 16 It can be seen from the figure that the use of polyamide plate can make each spot clearer, with less interference from miscellaneous peaks and accurate detection results.
[0109] The technical features of the above-described embodiments may be arbitrarily combined. To make the description concise, not all possible combinations of the technical features in the above-described embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.
[0110] The above-described embodiments only express several implementation methods of the present application, which is convenient for understanding the technical solution of the present application in detail, but it cannot be understood as limiting the scope of protection of the patent application. It should be pointed out that for ordinary technicians in this field, without departing from the concept of the present application, several modifications and improvements can be made, which all belong to the scope of protection of the present application. It should be understood that the technical solutions obtained by those skilled in the art through logical analysis, reasoning or limited experiments on the basis of the technical solutions provided in the present application are all within the scope of protection of the claims attached to the present application. Therefore, the scope of protection of the patent of this application shall be based on the content of the attached claims, and the description can be used to interpret the content of the claims.
Claims
1. A thin layer chromatography detection method for Cistanche deserticola in Cistanche epimedium black tea, characterized in that: The steps include: Prepare the test solution of Cistanche deserticola and Epimedium black tea, echinacea glycoside reference substance, verbascoside reference substance and Cistanche deserticola reference medicinal material solution; The test solution of the Cistanche epimedium black tea to be tested, the echinacoside reference substance, the verbascoside reference substance and the Cistanche deserticola reference medicinal material solution are spotted on the same polyamide thin layer plate, developed with a developing agent, taken out, dried and then subjected to color development treatment; Wherein, the developing agent is selected from the following: (1) ethanol, formic acid and water in a volume ratio of 2:(0.3-0.8):(6-10); (2) ethanol, acetic acid and water in a volume ratio of 2:(0.8-1.2):(6-10); (3) Methanol, acetic acid and water in a volume ratio of 1:(1.5-2.5):(6-10).
2. The thin layer chromatography detection method of Cistanche deserticola in Cistanche epimedium black tea according to claim 1, characterized in that: The developing agent is ethanol, formic acid and water in a volume ratio of 2:(0.3-0.8):(6-10).
3. The thin layer chromatography detection method of Cistanche deserticola in Cistanche epimedium black tea according to claim 1, characterized in that: The color development process includes a step of observation under ultraviolet light.
4. The thin layer chromatography detection method of Cistanche deserticola in Cistanche epimedium black tea according to claim 3, characterized in that: The wavelength of the ultraviolet lamp is 365nm.
5. The thin layer chromatography detection method of Cistanche deserticola in Cistanche epimedium black tea according to any one of claims 1 to 4, characterized in that: The test solution of Cistanche deserticola and Epimedium black tea to be tested comprises the following steps: Adding a first solvent to the tested Cistanche deserticola and Epimedium black tea for extraction, evaporating the obtained extract to dryness to prepare a first solid; The first solid is added into the second solvent to be dissolved, so as to prepare the test solution of the Cistanche deserticola and Epimedium black tea to be tested; And / or, preparing the Cistanche deserticola control medicinal material solution comprises the following steps: Adding a first solvent to a Cistanche deserticola reference medicinal material for extraction, and evaporating the obtained extract to dryness to prepare a first solid; The first solid is added into the second solvent to dissolve, so as to prepare the Cistanche deserticola control medicinal material solution.
6. The thin layer chromatography detection method of Cistanche deserticola in Cistanche epimedium black tea according to claim 5, characterized in that: The first solvent is each independently methanol.
7. The thin layer chromatography detection method of Cistanche deserticola in Cistanche epimedium black tea according to claim 5, characterized in that: The second solvent is each independently methanol.
8. The thin layer chromatography detection method of Cistanche deserticola in Cistanche epimedium black tea according to claim 5, characterized in that: Adding the first solvent for extraction includes the step of performing ultrasonic extraction; the conditions of the ultrasonic extraction include: a frequency of 30kHZ to 50kHZ and a power of 250W to 350W.
9. The thin layer chromatography detection method of Cistanche deserticola in Cistanche epimedium black tea according to claim 8, characterized in that: The ultrasonic extraction time is 5min to 25min.
10. The thin layer chromatography detection method of Cistanche deserticola in Cistanche epimedium black tea according to claim 5, characterized in that: The temperature for evaporating the obtained extract to dryness is 40°C to 50°C.