Preparation method of double-cycle breeding substrate based on waste bee hive

Abstract

The present application relates to the technical field of agricultural resource recycling, and particularly relates to a double-cycle breeding substrate preparation method based on waste bee guts; comprising the following steps: 1) waste bee gut pretreatment; 2) preparation of edible fungus breeding substrate; 3) edible fungus breeding; 4) waste edible fungus substrate treatment; 5) preparation of horticultural plant seedling substrate, used for plant seedling; the method solves the problems of resource waste and environmental pollution of agricultural waste, reduces waste emission, reduces new material mining, and inhibits potential pollution risks of soil, water and the like. Compared with the prior art, the application expands the application field of waste bee guts, realizes waste recycling, improves the nutritional composition of the substrate, reduces the production cost of the substrate, and has the advantages of environmental protection, economy, easy operation, wide applicability, improved resource utilization rate and the like, realizes the innovative combination of resource utilization and substrate recycling preparation of agricultural waste, and promotes the sustainable development of agriculture.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of agricultural resource recycling, in particular to a preparation method of a double-cycle breeding substrate based on waste bee spermatheca. BACKGROUND

[0002] In agricultural production and horticultural cultivation, traditional seedling substrates and cultivation substrates usually use materials such as peat, vermiculite, perlite, etc. However, peat is a non-renewable resource, and large-scale mining will lead to resource depletion. At the same time, materials such as vermiculite and perlite are difficult to degrade after use, and if not properly treated, they may cause secondary pollution to the environment.

[0003] In the beekeeping industry, bee colony management requires regular replacement of honeycomb, and a large amount of waste bee spermatheca is discarded. Waste bee spermatheca contains a large amount of bioactive components such as resin, oil, alkaloids, tannins, organic acids, amino acids, and proteins, and has various biological and pharmacological values. However, the current utilization of waste bee spermatheca mainly focuses on the recovery of beeswax and a few other aspects, and most of the waste bee spermatheca has not been fully utilized, resulting in resource waste.

[0004] Edible mushroom cultivation requires a large amount of substrate material. Currently commonly used substrate materials such as cottonseed hulls, sawdust, and straw, although widely available, have certain fluctuations in supply and nutrient content in large-scale production. Moreover, these used traditional substrates, if not properly disposed of, can also have certain impacts on the environment, such as land occupation, breeding of pests and pathogens, etc.

[0005] Existing horticultural plant seedling substrates have certain limitations in terms of cost, air permeability, water retention, nutrient supply, etc. Some substrates are relatively expensive, increasing the cost of seedling cultivation. Some substrates have poor air permeability, which can easily lead to root hypoxia, affecting seedling growth. Some substrates have poor water retention, requiring frequent watering, increasing labor intensity and wasting water resources. In addition, uneven nutrient supply in the substrate also affects the quality and growth rate of seedlings. SUMMARY

[0006] The present application provides a preparation method of a double-cycle breeding substrate based on waste bee spermatheca to solve the problem of not maximizing the utilization of waste bee spermatheca, and the technical problems of high production cost, poor air and water permeability, uneven nutrient supply, resource and environmental pollution after disposal, non-recyclable, and non-sustainable utilization of traditional edible mushroom cultivation substrates and horticultural plant seedling substrates.

[0007] The waste bee honeycomb is prepared into a recycling breeding substrate, the waste bee honeycomb is first converted into an edible fungus breeding substrate, and is used for edible fungus cultivation, which can not only provide a suitable environment and nutrients for the growth of edible fungi, but also after the edible fungus cultivation, the waste edible fungus substrate containing the bee honeycomb is used for the preparation of a horticultural plant seedling substrate, the nutrient components in the waste edible fungus substrate are decomposed and transformed by the edible fungus, and become more easily absorbed and utilized by the horticultural plants. The recycling utilization mode can realize the maximum utilization and recycling of resources, reduce the discharge of waste, reduce the pressure on the environment, and can also reduce the cost of edible fungus cultivation and horticultural plant seedling, improve the breeding quality, and has significant economic and environmental benefits.

[0008] To solve the above technical problems, the technical scheme adopted by the present application is as follows: a double-cycle breeding substrate preparation method based on waste bee honeycomb, comprising the following steps:

[0009] 1) waste bee honeycomb pretreatment: collect waste bee honeycomb, remove surface impurities, residual beeswax and propolis by combining manual cleaning with hot water soaking, after soaking, perform sufficient rinsing operation, then dry and crush into uniform particles, and finally sterilize the particles in a high-temperature and high-pressure sterilization pot;

[0010] 2) preparation of edible fungus breeding substrate: mix the pretreated waste bee honeycomb particles with wood chips, cottonseed hulls, straw and corn cob, which are conventional edible fungus substrate raw materials, add an appropriate amount of water, control the moisture content of the substrate at 60%-70%, and stir uniformly;

[0011] 3) edible fungus breeding: after stirring uniformly, load into edible fungus cultivation bags or bottles, perform conventional edible fungus inoculation operation in a sterile environment, then transfer the inoculated cultivation container to a culture room with controllable temperature, humidity and light conditions for cultivation, and finally collect according to the maturity standard of edible fungus;

[0012] 4) waste edible fungus substrate treatment: after the edible fungus is harvested, collect the waste edible fungus substrate and transport it to a composting site, perform turning operation every 3-5 days during composting to ensure that the internal and external materials are fully mixed and oxygen is exchanged, and the fermentation time lasts for 30-45 days until the substrate is composted;

[0013] 5) preparation of horticultural plant seedling substrate: use a crushing machine to crush the waste edible fungus substrate composted in step 4), accurately control the parameters during the process to directly crush it to meet the particle size standard of the horticultural plant seedling substrate, then screen it by means of a vibrating screen to effectively remove large impurities, and according to the requirements of different horticultural plant species on the substrate during seed germination, cutting rooting and seedling growth, scientifically add mineral additives to the seedling substrate according to the weight proportion, adjust the air permeability and water retention of the substrate to obtain the seedling substrate, and use it for plant seedling.

[0014] As a further limitation of the technical scheme of the application, the artificial cleaning in step 1) is to gently brush the surface of the bee gut with a brush to remove some loosely attached impurities, wax debris and propolis particles.

[0015] As a further limitation of the technical scheme of the application, the temperature of hot water soaking in step 1) is 85℃±2℃, and the soaking time is 45 minutes.

[0016] As a further limitation of the technical scheme of the application, the drying temperature in step 1) is maintained at 35℃±2℃, and the drying time is 12-24 hours (the specific duration needs to be adjusted according to the initial moisture content of the bee gut, environmental ventilation conditions, and the stacking thickness of the bee gut, etc.), so that it reaches a moisture content of 12%-15%. During the drying process, the bee gut is turned over every 2-3 hours to ensure uniform drying. After drying, the particle size of the crushed bee gut is 1-3 centimeters.

[0017] As a further limitation of the technical scheme of the application, the high-temperature high-pressure sterilization conditions are: sterilization treatment for 30-60 minutes in the temperature range of 120-130℃ and the pressure range of 0.1-0.2MPa.

[0018] As a further limitation of the technical scheme of the application, the pretreated waste bee gut particles in step 2) account for 20%-40% of the total mass of the edible mushroom substrate raw materials.

[0019] As a further limitation of the technical scheme of the application, the conventional edible mushroom substrate raw materials in step 3) are sawdust, cottonseed hulls, straw or corn cobs.

[0020] As a further limitation of the technical scheme of the application, the judgment standard for maturity in step 4) is that the color changes to dark brown, the texture is loose and porous, and there is no pungent odor.

[0021] As a further limitation of the technical scheme of the application, the mineral additive in step 5) is perlite or vermiculite.

[0022] The application deeply resources and cyclically utilizes the waste and old bee guts, removes the waste and old bee gut impurities, beeswax and propolis through artificial cleaning and hot water soaking, and after crushing and high temperature and high pressure sterilization, the waste and old bee guts are mixed with conventional raw materials such as wood chips to make edible fungus breeding substrate, edible fungus is inoculated and cultivated, and edible fungus is harvested, and then the harvested edible fungus waste and old substrate is composted, crushed and sieved to make garden plant seedling substrate. The method solves the problems of resource waste and environmental pollution of agricultural waste, reduces waste discharge, reduces new material mining, and inhibits potential pollution risks of soil, water and the like. Compared with the prior art, the application expands the application field of waste and old bee guts, realizes waste recycling, improves the water holding and air permeability of the substrate and the nutritional ingredients, reduces the substrate manufacturing cost, and has the advantages of environmental protection, economy, easy operation, wide applicability, improved resource utilization rate and the like, realizes the innovative combination of resource utilization and substrate recycling preparation of agricultural waste, and promotes the development of agriculture.

[0023] Compared with the prior art, the application has the following beneficial effects:

[0024] The application changes the traditional single substrate utilization mode to a double recycling mode, and converts the agricultural waste of waste and old bee guts into a high value resource. The waste and old bee guts are treated by combining artificial cleaning and hot water soaking to remove waste and old impurities, beeswax and propolis, and ensure the purity of the subsequent processing raw materials. High temperature and high pressure sterilization technology is used to kill potential pathogens, insect eggs and other harmful organisms, and provide a safe environment for the growth of subsequent edible fungus and garden plants. A specific composting and fermentation process is used to convert the waste and old edible fungus substrate into high-quality garden plant seedling substrate, improve the utilization rate of waste and old bee guts and edible fungus substrate, improve the yield and quality of edible fungus, and improve the germination rate and survival rate of garden plants.

[0025] The application utilizes the porous structure of bee intestines to improve the air permeability of the substrate, and the fiber pores of conventional raw materials such as wood chips form a complementary effect to supply sufficient oxygen to microorganisms and plant root systems, promote respiration and metabolism, and promote the growth of edible fungi and the development of horticultural plant root systems; the bee intestines contain various hydrophilic natural substances and water-holding pores, which can effectively regulate the water retention of the substrate, continuously optimize the physical structure of the substrate, and avoid root rot caused by water accumulation. At the same time, the bee intestines are rich in nutrients such as protein, amino acids, minerals, resins, oils, alkaloids and bioactive components, which can balance the needs of edible fungi and horticultural plants for the growth environment under the control of temperature, humidity, light and other environmental conditions, without the need for chemical additives and high-cost imported substrates. Under suitable conditions, it promotes the rapid growth of edible fungus mycelium and the germination of horticultural plant seeds, and the yield of edible fungi increases by 10%-20%, and the germination rate of horticultural plant seeds is more than 90%. The bee intestines used in the application are gradually decomposed during substrate preparation and composting, providing continuous nutrients for the growth of edible fungi and plants, and the bioactive components in the bee intestines can also promote the reproduction of beneficial microorganisms, inhibit harmful pathogens, and reduce the probability of disease and pest occurrence. In addition, the application of the substrate to the soil can improve the soil aggregate structure, enhance the soil stability and buffering capacity, and maintain the soil fertility in the long term. The application utilizes waste bee intestines to prepare edible fungus substrate and horticultural plant substrate, meets the substrate breeding requirements, and effectively solves the technical problems of resource waste, environmental pollution, high substrate cost, poor water retention and air permeability, and unbalanced nutrient supply in the process of existing edible fungus cultivation and horticultural plant seedling cultivation.

[0026] Compared with the prior art, the application fully considers resource recycling in substrate preparation, utilizes the unique nutrient components and structural properties of waste bee intestines to improve the air permeability and water retention of the substrate, makes the nutrient components of the substrate more balanced, shortens the time for edible fungus mycelium to fill the bag and the horticultural plant seedling cycle, and the cost of substrate cost investment in the early stage of breeding is lower. The entire recycling process does not require a large amount of new resources and high-energy consumption equipment, and the comprehensive cost is low. In summary, the application has the advantages of high utilization rate of agricultural waste, simple preparation process, low production cost, good breeding effect, environmental protection and sustainability, and many other advantages. DETAILED DESCRIPTION

[0027] The application will be further described below in conjunction with specific embodiments.

[0028] In this embodiment, a double-cycle breeding substrate preparation method based on waste bee intestines includes the following steps:

[0029] 1) Pretreatment of Used Honeycombs: Collect used honeycombs and first brush away impurities, wax debris, and propolis particles adhering to their surfaces. Then, soak the honeycombs in 85°C hot water for 45 minutes to further remove any remaining wax and propolis and ensure effective cleaning. After soaking, rinse thoroughly. After rinsing, dry the honeycombs in a well-ventilated environment at 35°C for 12 hours, until they reach a moisture content of 15%. During the drying process, turn the honeycombs every two hours to ensure even drying.

[0030] 2) Use a crusher with precise particle size control to crush it into particles with a size of about 1-3 cm. Place the crushed honeycomb particles in an intelligent high-temperature and high-pressure sterilizer, set the sterilization temperature to 125°C, the pressure to 0.15 MPa, and the sterilization time to 30 minutes.

[0031] 3) Prepare the edible fungus cultivation substrate: Mix 20% pretreated spent honeycomb pellets with 60% selected cottonseed hull pellets and 20% dried corncob pellets to form the edible fungus substrate. Add an appropriate amount of water to maintain a stable moisture content of 60%.

[0032] 4) Shiitake Mushroom Cultivation: Mix thoroughly with a powerful blender, then place into edible mushroom cultivation bags. Inoculate the shiitake mushroom spawn in a Class 100 sterile inoculation chamber, using a dosage of 5-10 grams per bag. After inoculation, transfer the bags to a controlled incubation room maintained at a temperature of 20-25°C, a humidity of 60%-70%, and a light intensity of 500-1000 lx. The incubation period is approximately 60-90 days, until the mushrooms reach maturity and are ready for harvest. Results show that using a substrate supplemented with used honeycombs for shiitake mushroom cultivation results in rapid mycelial germination, with bags filled in 28-30 days. During growth, the fruiting bodies develop robust growth, with thick caps measuring 6-6.5 cm in diameter and stipes averaging 1.6-1.8 cm in diameter. Each bag yields 1.0-1.1 kg of shiitake mushrooms.

[0033] 5) Disposal of Wasted Mushroom Substrate: After mushroom harvesting, waste mushroom substrate is collected and piled into a standard compost pile approximately 1.5 meters wide and 1 meter high. The pile is turned every four days. After 35 days of fermentation, the substrate is decomposed to a dark brown color, with a loose, porous texture and no pungent odor.

[0034] 6) Prepare horticultural plant seedling medium: Process the decomposed medium through efficient crushing and fine screening equipment to obtain a horticultural plant seedling medium. Add 20% high-quality perlite to the medium, mix thoroughly, and fill it into a 72-hole tray for cucumber seedling cultivation.

[0035] 7) Cucumber Seedling Raising: Protect from light during the germination period, maintain an ambient temperature of 25-30°C, maintain a substrate humidity of 60%-70%, and maintain an air humidity of 70%-80%. During the seedling stage, maintain a daytime temperature of 22-25°C and a nighttime temperature of 15-18°C, maintain a substrate humidity of 50%-60%, and maintain an air relative humidity of 60%-70%. Provide 10-12 hours of sunlight per day at an intensity of 2000-5000 lx, with artificial supplemental lighting as needed. Results show that cucumber seed germination rates can exceed 90%, seedlings grow robustly, and transplanted seedlings have well-developed root systems with root lengths of 12-15 cm.

[0036] Example 2

[0037] In this embodiment, a method for preparing a double-circulation breeding matrix based on waste honeycombs includes the following steps:

[0038] 1) Pretreatment of used honeycombs: Used honeycombs are collected from professional bee farms and the surface impurities, residual beeswax and propolis are removed by combining manual cleaning and hot water soaking.

[0039] 2) Use a crusher with precise particle size control to crush it into particles with a size of about 1-3 cm. Place the crushed honeycomb particles in an intelligent high-temperature and high-pressure sterilizer, set the sterilization temperature to 125°C, the pressure to 0.15 MPa, and the sterilization time to 45 minutes.

[0040] 3) Preparation of edible fungus breeding matrix: Mix 20% of pretreated waste honeycomb particles with 60% of selected cottonseed hulls and 20% of dried corn cobs as the edible fungus matrix raw materials, and adjust the matrix moisture content to 60%.

[0041] 4) Oyster Mushroom Propagation: Place the substrate into a vented cultivation bottle and inoculate the oyster mushroom spawn under a sterile environment at a rate of 3-8 grams per bottle. After inoculation, place the bottle in a culture room at a temperature of 18-23°C, a humidity of 65%-75%, and a light intensity of 300-800 lx. The incubation period is approximately 40-60 days, and the mushrooms are harvested when mature. Results show that it takes 20-25 days for the mycelium to fill the bottle. As the mushrooms grow, their caps reach a diameter of 8-10 cm, with a uniform thickness and firm texture. The stems are short and sturdy, with an average diameter of 1.8-2.2 cm. The yield per bottle is 1.2-1.4 kg.

[0042] 5) Disposal of Wasted Mushroom Substrate: After the oyster mushrooms are harvested, the spent mushroom substrate is immediately collected and piled into a standard compost pile approximately 1.5 meters wide and 1 meter high. This pile is turned every four days using an automated compost turner. After 35 days of fermentation, the substrate matures to a dark brown color, becomes loose and porous, and emits no pungent odor.

[0043] 6) Preparation of nursery substrate for horticultural plants: the composted substrate is processed by high-efficiency crushing and fine screening equipment to obtain the nursery substrate for horticultural plants. 20% of perlite is added to the nursery substrate, and after being thoroughly mixed, it is filled into 72-hole plug trays for tomato seedling.

[0044] 7) Tomato seedling: during the germination period, the substrate humidity is 60%-70%, the air humidity is 70%-80%, the temperature is controlled at 25-30°C, and a small amount of scattered light is maintained; during the seedling period, the daytime temperature is 20-25°C, the nighttime temperature is 15-18°C, the substrate humidity is 50%-60%, the air humidity is 60%-70%, the daily light duration is 12-14 hours, and the intensity is 3000-5000 lx, and the light is supplemented by fluorescent lamps when necessary. The results show that the average height of the transplanted tomato seedlings is 16-19 cm, and the stem diameter is 0.4-0.5 cm.

[0045] Example 3

[0046] The preparation method of the double-cycle breeding substrate based on waste bee intestines in this embodiment includes the following steps:

[0047] 1) Pretreatment of waste bee intestines: collect waste bee intestines from professional beekeeping farms, first remove larger impurity particles by mechanical vibration screening, then carefully remove residual beeswax, propolis and other impurities attached to the surface by hand. Use a pulverizer with precise particle size control function to crush the waste bee intestines into particles with a particle size of about 1-3 cm.

[0048] 2) Place the crushed bee intestine particles in an intelligent high-temperature and high-pressure sterilization pot, set the sterilization temperature to 125°C, the pressure to 0.15 MPa, and the sterilization time to 45 minutes.

[0049] 3) Preparation of edible mushroom breeding substrate: take 40% of the waste bee intestine particles and mix them with 40% of high-quality sawdust and 20% of pure rice husk as the edible mushroom breeding substrate.

[0050] 4) Adjust the moisture content of the mechanism to 70%, inoculate the Flammulina velutipes strain under sterile conditions, with an inoculation amount of 8-12 grams per bag, fill the special cultivation bag, and place it in a culture room with a temperature of 8-15°C, a humidity of 80%-90%, and a dark environment for cultivation, with a cultivation period of about 90-120 days. The results show that the Flammulina velutipes mycelium grows vigorously, the mycelium full-bag time is 35-40 days, the fruiting body grows in an orderly manner, the cap diameter can reach 0.6-0.9 cm, the stem is slender and uniform, the average diameter is 0.2-0.4 cm, and the length is 13-18 cm, and the yield of Flammulina velutipes per bag is 0.7-1.0 kg.

[0051] 5) Waste edible mushroom substrate treatment: After the harvest of the golden needle mushroom, the waste edible mushroom substrate is immediately collected and stacked into a standard compost pile with a width of about 1.5 meters and a height of about 1 meter. An automatic turning machine is used to turn the pile every 4 days. After 35 days of fermentation treatment, the substrate is fully decomposed to a dark brown color, with a loose and porous texture, and no pungent odor.

[0052] 6) Preparation of horticultural plant seedling substrate: The decomposed substrate is processed by high-efficiency crushing and fine screening equipment to obtain a horticultural plant seedling substrate. Add 15% vermiculite as a horticultural plant seedling substrate for Sedum cuttage. Keep the substrate humidity at 30%-40%, the air humidity at 50%-65%, and the environmental temperature at 25-32℃. The Sedum short shoot is cultivated under the following conditions: 50% shade between 12:00-14:00 and 30% shade at other times, for 25-35 days. The results show that the Sedum cuttage seedlings rooted in 7-11 days, with a rooting rate of 99%, and grew well. The cuttage seedlings had developed root systems, with an average root length of 4-7 cm, and the plants were healthy, with green and thick leaves.

[0053] Example 4

[0054] The preparation method of the double-cycle breeding substrate based on waste bee intestines in this embodiment includes the following steps:

[0055] 1) Waste bee intestine pretreatment: Collect waste bee intestines from professional bee farms. First, remove larger impurity particles by mechanical vibration screening, and then carefully remove residual beeswax, propolis and other impurities attached to the surface by hand. Use a crusher with precise particle size control function to crush the bee intestines into particles with a particle size of about 1-3 cm.

[0056] 2) High temperature and high pressure sterilization: Place the crushed bee intestine particles in an intelligent high temperature and high pressure sterilization pot, set the sterilization temperature to 125℃, the pressure to 0.15MPa, and the sterilization time to 60 minutes.

[0057] 3) Preparation of edible mushroom cultivation substrate: The pretreated waste bee comb particles are mixed with 30% and 50% of selected sawdust, 20% of dried straw as the raw material of the edible mushroom substrate. Add an appropriate amount of water to stabilize the moisture content of the substrate at 65%. After uniform stirring with a powerful stirrer, load it into a specially designed edible mushroom cultivation bag, inoculate the agaricus bisporus spores in a class 100 sterile inoculation room, and the inoculation amount is calculated as 6-9 grams of spores per bag. After inoculation, the cultivation bag is transferred to a culture room with a temperature control of 22-26°C, a humidity of 65%-75%, and a light intensity of 300-600lx. The culture period is about 70-100 days. The results show that the use of this substrate to cultivate agaricus bisporus has a fast mycelium germination speed, and the mycelium full-bag time is 30-35 days. During the growth of agaricus bisporus, the ear pieces are large and thick, with an average diameter of 6-8 centimeters, the ear piece edges are neat, the color is black and bright, and the yield of agaricus bisporus per bag reaches 1.0-1.3 kilograms.

[0058] 4) Treatment of waste edible mushroom substrate: After the harvest of agaricus bisporus is completed, the waste edible mushroom substrate is immediately collected and stacked into a standard compost pile with a width of about 1.5 meters and a height of about 1 meter. The automatic turning machine is used to turn the pile every 4 days. After 40 days of fermentation treatment, the substrate is composted to dark brown, with loose and porous texture, and no pungent odor. This is due to the optimization of the structure and composition of the waste bee comb, which makes the fermentation process more complete and stable, providing a good foundation for subsequent substrate reuse.

[0059] 5) Preparation of horticultural plant seedling substrate: The composted substrate is treated by high-efficiency crushing and fine screening equipment to obtain a horticultural plant seedling substrate. Add 18% of perlite and 5% of vermiculite to the seedling substrate, mix thoroughly, and use it for iris seedling. The seedling environment maintains a temperature of 20-25°C, a substrate humidity of 50%-60%, an air humidity of 60%-70%, and a daily light duration of 8-10 hours with a light intensity of 2000-3000lx. The results show that the germination rate of iris seeds can reach more than 92%, and the seedlings grow straight and healthy with green leaves.

[0060] Comparative Example 1

[0061] The difference between the preparation method of the agaricus bisporus and cucumber seedling substrate in this comparative example and Example 1 is that waste bee comb is not used, and other cultivation conditions remain the same.

[0062] 1) Substrate raw material preparation: Selected cottonseed hulls and dried corn cobs are used as the raw material of the edible mushroom substrate.

[0063] 2) Preparation of edible mushroom cultivation substrate: 80% cotton seed shell particles and 20% dried corn cob particles are mixed, and then an appropriate amount of water is added to stabilize the moisture content of the substrate at 60%. After uniform stirring with a powerful stirrer, the substrate is loaded into edible mushroom cultivation bags, and Lentinula edodes spores are inoculated in a Class 100 sterile inoculation room, with an inoculation amount of 5-10 grams of spores per bag.

[0064] 3) Lentinula edodes cultivation: After inoculation, the cultivation bags are transferred to a culture room with a temperature controlled at 20-25°C, a humidity maintained at 60%-70%, and a light intensity of 500-1000 lx for cultivation. The cultivation period is about 60-90 days, and the mushrooms are harvested when they reach the harvesting standard. The results show that the mycelium germination speed is slow, and the mycelium full-bag time is extended to 32-35 days. During the growth of Lentinula edodes, the fruiting body grows relatively weak, with a cap diameter of 5-6 cm, a thickness that is slightly thin, and an average stem diameter of 1.4-1.6 cm. The yield of Lentinula edodes per bag is 0.9-1.0 kg.

[0065] 4) Treatment of waste edible mushroom substrate: After the harvest of Lentinula edodes, the waste mushroom substrate is collected and stacked into standard compost piles with a width of about 1.5 meters and a height of about 1 meter. The piles are turned over every 4 days. After 35 days of fermentation treatment, the substrate is composted to a dark brown color, and the texture is loose and porous. However, the odor is relatively heavier during the composting process.

[0066] 5) Preparation of horticultural plant seedling substrate: The composted substrate is treated by high-efficiency crushing and fine screening equipment to obtain a horticultural plant seedling substrate. 20% of high-quality perlite is added to the seedling substrate, and after thorough mixing, it is filled into 72-hole plug trays for cucumber seedling.

[0067] 6) Cucumber seedling: During the germination period, avoid light, and maintain an environmental temperature of 25-30°C, a substrate humidity of 60%-70%, and an air humidity of 70%-80%. During the seedling period, the daytime temperature is controlled at 22-25°C, and the nighttime temperature is 15-18°C. The substrate humidity is maintained at 50%-60%, and the air relative humidity is controlled at 60%-70%. The daily light period is 10-12 hours, and the intensity is 2000-5000 lx, and artificial light is supplemented as needed. The cucumber seed germination rate is about 80%-85%. The seedlings grow relatively weak, and the root length of the transplanted seedlings is 8-12 cm.

[0068] In the above comparative example, the edible mushroom substrate raw material is only composed of 80% cottonseed hull particles and 20% dried corncob particles, lacking the special nutrient supplement from the waste bee guts. In Example 1, the waste bee guts are pretreated, crushed and sterilized, and then mixed with 60% selected cottonseed hull particles and 20% dried corncob particles at a ratio of 20% to serve as the edible mushroom substrate raw material. This makes the substrate not only have the regular nutrients provided by cottonseed hulls and corncobs, but also provides unique nutrients for the growth of edible mushrooms due to the rich protein, amino acids, minerals and other components in the waste bee guts. Compared with the two methods, the raw material composition of Example 1 is richer, and the substrate containing waste bee guts can provide a more comprehensive nutrient basis for the subsequent growth of edible mushrooms, creating conditions for cultivating higher quality edible mushrooms.

[0069] In the above comparative example, due to the lack of waste bee guts in the substrate, the mycelium germination speed is slow, the mycelium full-bag time is extended to 32-35 days, the growth of the mushroom fruiting body is relatively weak, the cap diameter is 5-6 cm, the thickness is slightly thin, the average stem diameter is 1.4-1.6 cm, and the yield of mushroom per bag is 0.9-1.0 kg. In Example 1, when preparing the edible mushroom breeding substrate, the addition of waste bee guts optimizes the physical structure of the substrate, and the air permeability and water retention are better. In the process of mushroom breeding, the mycelium germination speed is fast, the mycelium full-bag time is 28-30 days, the growth of the mushroom fruiting body is healthy, the cap is thick and the diameter is 6-6.5 cm, the average stem diameter reaches 1.6-1.8 cm, and the yield of mushroom per bag reaches 1.0-1.1 kg. Compared with the two methods, Example 1 performs better in all indicators of mushroom breeding, and the waste bee guts have a significant effect on promoting the growth of mushrooms and improving yield and quality.

[0070] In the above comparative example, although the substrate is also decomposed to dark brown and has loose and porous texture, the odor is relatively heavier during the decomposition process, which indicates that the substrate lacking waste bee guts has different microbial decomposition components and processes during fermentation, resulting in poor odor control. In Example 1, after the waste mushroom substrate is treated after the end of mushroom harvesting, it is fermented for 35 days, and the substrate is decomposed to dark brown, has loose and porous texture, and does not emit a pungent odor. This is due to the improvement of the substrate structure and composition by the waste bee guts in the early stage, making the fermentation process more complete and stable. Compared with the two methods, the treatment effect of Example 1 on the waste edible mushroom substrate is better, which is more conducive to subsequent reuse and reduces the adverse effects on the environment.

[0071] In the above comparative example, the germination rate of cucumber seeds was about 80%-85%, and the seedlings grew relatively weakly. The root length of the transplanted seedlings was 8-12 cm. Because the substrate raw material did not use waste bee guts, the lack of nutritional components and physical structure affected the effect of cucumber seedling raising. In Example 1, 20% high-quality perlite was added to the substrate containing waste bee guts after composting, which was used for cucumber seedling raising. The germination rate of cucumber seeds could reach more than 90%, and the seedlings grew vigorously. The root system of the transplanted seedlings was developed, and the root length was 12-15 cm. This was because the substrate constructed by the waste bee guts in the early stage retained part of the nutritional components and good physical structure after composting, providing a suitable environment for cucumber seedling raising. Compared with the two methods, Example 1 showed obvious advantages in the seedling raising of horticultural plants, which again proved the positive role of waste bee guts in improving the quality of subsequent seedling raising in the whole cycle of breeding.

[0072] Comparative Example 2

[0073] The difference between the preparation method of the oyster mushroom and tomato seedling substrate in this comparative example and Example 2 is that waste bee guts are not used, and other breeding conditions remain the same.

[0074] 1) Preparation of edible fungus breeding substrate: 80% of selected cottonseed hulls and 20% of dried corn cob are mixed as edible fungus substrate raw material, and the water content of the substrate is adjusted to 60%.

[0075] 2) Oyster mushroom breeding: The substrate is loaded into a cultivation bottle with air holes, and oyster mushroom spores are inoculated in a sterile environment, with an inoculation amount of 3-8 grams per bottle. After inoculation, the cultivation bottle is placed in a culture room with a temperature of 18-23°C, a humidity of 65%-75%, and a light intensity of 300-800 lx. The culture time is about 40-60 days, and the oyster mushrooms are harvested when they are mature. The results show that the oyster mushroom mycelium fills the bottle in 25-30 days, the cap diameter is 68 cm, the stem is relatively thin with an average diameter of 1.5-2 cm, and the yield of oyster mushrooms per bottle is reduced to 1-1.3 kg.

[0076] 3) Treatment of waste edible fungus substrate: After the oyster mushroom harvest is completed, the waste edible fungus substrate is immediately collected and stacked into a standard compost pile with a width of about 1.5 meters and a height of about 1 meter. The automatic turning machine is used to turn the pile every 4 days. After 35 days of fermentation treatment, the substrate is composted to dark brown, with loose and porous texture, and no pungent odor.

[0077] 4) Preparation of horticultural plant seedling substrate: The composted substrate is treated by high-efficiency crushing and fine screening equipment to obtain a horticultural plant seedling substrate. 20% of perlite is added to the seedling substrate, and after mixing, it is filled into a 72-hole plug tray for tomato seedling raising.

[0078] 5) Tomato seedling: during the germination period, the substrate humidity is 60%-70%, the air humidity is 70%-80%, the temperature is controlled at 25-30°C, and a small amount of scattered light is maintained; during the seedling period, the temperature is 20-25°C during the day and 15-18°C at night, the substrate humidity is 50%-60%, the air humidity is 60%-70%, the light is 12-14 hours per day, and the intensity is 3000-5000lx, supplemented by fluorescent light when necessary. The results show that the average height of the transplanted tomato seedlings is 15-17 cm, and the stem diameter is 0.3-0.4 cm.

[0079] In the above comparative examples, Comparative Example 2 only uses 80% selected cottonseed hulls and 20% dried corn cobs as raw materials, lacking the special nutrients provided by the waste bee guts. Example 2 uses the waste bee guts after pretreatment at a proportion of 20% mixed with 60% selected cottonseed hulls and 20% dried corn cobs as the raw materials for the edible mushroom substrate. The waste bee guts are rich in protein, amino acids, and minerals, adding unique nutritional components to the substrate and enriching the raw material composition. Compared with the two methods, the raw material composition of Example 2 is more abundant, providing more comprehensive nutritional support for the growth of edible mushrooms, which helps to cultivate higher-quality edible mushrooms.

[0080] In the above comparative examples, the time for the mushroom mycelium to fully cover the bottle in Comparative Example 2 is extended to 25-30 days, the cap diameter is 6-8 cm, and the stem diameter is 1.5-2 cm on average, with a yield of 1-1.3 kg per bottle. In Example 2, waste bee guts are added when preparing the edible mushroom breeding substrate, optimizing the physical structure of the substrate and making it more breathable and water-retentive. During the breeding process of the oyster mushrooms, the time for the mycelium to fully cover the bottle is 20-25 days, the cap diameter of the oyster mushrooms can reach 8-10 cm, the cap thickness is uniform and the flesh is tight, the stem is short and stout, the diameter reaches 1.8-2.2 cm on average, and the yield of oyster mushrooms per bottle is 1.2-1.4 kg. Compared with the two methods, Example 2 performs better in all indicators of oyster mushroom breeding, showing that waste bee guts can promote the growth of oyster mushrooms, improve yield, and improve quality.

[0081] In the above comparative examples, although Comparative Example 2 uses the same treatment method, the substrate is also decomposed to a dark brown color and has a loose and porous texture without a pungent odor, but due to the lack of waste bee guts, there may be slight differences in the composition and process of microbial decomposition during fermentation. In Example 2, after the oyster mushrooms are harvested, the waste edible mushroom substrate is treated and fermented for 35 days. The substrate is decomposed to a dark brown color, has a loose and porous texture, and does not emit a pungent odor. This is due to the improvement of the substrate structure and composition by the waste bee guts in the early stage, making the fermentation process more complete and stable. Compared with the two methods, Example 2 also performs well in the treatment effect of waste edible mushroom substrate, providing a good foundation for subsequent substrate reuse.

[0082] In the above comparative examples, Comparative Example 2 had an average height of 15-17 cm and a stem diameter of 0.3-0.4 cm under the same seedling environment. In Example 2, the rotten substrate was added with 20% perlite for tomato seedling, and the results showed that the average height of the transplanted seedlings was 16-19 cm and the stem diameter was 0.4-0.5 cm. This was due to the substrate constructed by the waste bee guts, which retained the nutrient composition and good physical structure after rotting, creating a suitable environment for tomato seedling. Compared with the two methods, Example 2 had more advantages in the seedling of horticultural plants, and the waste bee guts played a role in improving the quality of subsequent seedling in the whole cycle of breeding.

[0083] Comparative Example 3

[0084] In the present comparative example, the preparation method of the substrate for the cultivation of Flammulina velutipes and Sedum was different from Example 3 in that waste bee guts were not used, and other breeding conditions remained the same.

[0085] 1) Preparation of edible fungus breeding substrate: 80% high-quality sawdust and 20% pure rice husk were mixed as the edible fungus breeding substrate, and the waste bee guts were not used.

[0086] 2) Adjust the moisture content to 70%, inoculate the Flammulina velutipes spores under sterile conditions, the inoculation amount is 8-12 grams per bag, and then place it in a special cultivation bag in a culture room with a temperature of 8-15°C, a humidity of 80%-90%, and a dark environment. The culture period is about 90-120 days. The results showed that the growth rate of Flammulina velutipes mycelium was general, the mycelium full bag time was 45-50 days, the fruiting body growth was relatively not uniform, the cap diameter could reach 0.5-0.8 cm, the stem thickness was slightly uneven, the average diameter was 0.1-0.3 cm, and the length was 10-15 cm. The yield of Flammulina velutipes per bag was 0.5-0.9 kg.

[0087] 3) Waste edible fungus substrate treatment: After the Flammulina velutipes is harvested, the waste edible fungus substrate is immediately collected and stacked into a standard compost pile with a width of about 1.5 meters and a height of about 1 meter. The automatic turning machine is used to turn the pile every 4 days. After 35 days of fermentation treatment, the substrate is rotten to dark brown, and the texture is loose and porous. The smell is slightly heavy during the rotting process.

[0088] 4) Preparation of nursery substrate for horticultural plants: the composted substrate is processed by high-efficiency crushing and fine screening equipment to obtain the nursery substrate for horticultural plants. Add 15% vermiculite as the nursery substrate for horticultural plants for Sedum cutting seedling. Keep the substrate humidity at 30%-40% and the air humidity at 50%-65% after cutting, control the environmental temperature at 25-32℃, and cultivate the Sedum short shoots in the environment with 50% shading degree between 12:00-14:00 and 30% shading degree at other times, the cultivation time is 25-35 days. The results show that the Sedum cutting seedlings have a rooting rate of 99% and the average root length of the transplanted seedlings is 3-6 cm.

[0089] In the above comparative example, 80% high-quality sawdust is mixed with 20% pure rice husk. In Example 3, waste bee guts are collected, mechanically vibrated, manually cleaned and crushed, and 40% of them are mixed with 40% high-quality sawdust and 20% pure rice husk to provide rich nutrients such as proteins, amino acids and minerals for the substrate. Compared with the two methods, the substrate raw materials of Example 3 are more abundant and the nutrient structure is more perfect, laying a better material foundation for subsequent cultivation.

[0090] In the above comparative example, the growth rate of the Flammulina velutipes mycelium is general, the bag-filling time is 45-50 days, the fruiting body growth is not uniform, the cap diameter is 0.5-0.8 cm, the stem diameter is uneven, the diameter is 0.1-0.3 cm, and the length is 10-15 cm, and the yield per bag is 0.5-0.9 kg. In Example 3, due to the use of substrate added with waste bee guts, the Flammulina velutipes mycelium grows vigorously, the bag-filling time is 35-40 days, the fruiting body grows uniformly, the cap diameter is 0.6-0.9 cm, the stem is slender and uniform, the diameter is 0.2-0.4 cm, the length is 13-18 cm, and the yield per bag is 0.7-1.0 kg. Compared with the two methods, Example 3 has more advantages in the growth rate, fruiting body morphology and yield of Flammulina velutipes, indicating that waste bee guts can effectively promote the growth and development of Flammulina velutipes.

[0091] In the above comparative example, the substrate is also composted to dark brown and has loose and porous texture, but the odor is slightly heavy during the composting process. In Example 3, after the Flammulina velutipes is harvested, the waste substrate is composted and fermented, and after 35 days, it is composted to dark brown, has loose and porous texture, and has no pungent odor. Compared with the two methods, in Example 3, the waste bee guts promote the optimization of the substrate structure and composition, and the treatment effect of the waste edible mushroom substrate is better, making the fermentation process more sufficient and stable, and more conducive to subsequent reuse.

[0092] In the above comparative example, the Sedum cuttings were rooted in 7-13 days, with a rooting rate of 99%, and the average root length of the transplanted seedlings was 3-6 cm. In Example 3, 15% vermiculite was added to the rotten substrate for Sedum cutting seedling raising. Under specific temperature, humidity and shade conditions, the Sedum cutting seedlings were rooted in 7-11 days, with a rooting rate of 99%, and the root system was developed. The average root length of the transplanted seedlings was 4-7 cm, the plants were healthy, and the leaves were green and thick. Compared with the two methods, the Sedum cutting seedlings in Example 3 had shorter rooting time, better root system and plant growth, which showed the positive effect of the recycled breeding substrate containing waste bee guts on the seedling raising of horticultural plants.

[0093] Comparative Example 4

[0094] The preparation method of the agaric and iris seedling substrate in the present comparative example is different from that in Example 4 in that waste bee guts are not used, and other breeding conditions remain the same.

[0095] 1) Preparation of edible fungus breeding substrate: 80% of selected sawdust and 20% of dried straw are mixed as raw materials for edible fungus substrate, and waste bee guts particles are not used. Add an appropriate amount of water to stabilize the moisture content of the substrate at 65%. After uniform stirring with a powerful stirrer, load it into a specially designed edible fungus cultivation bag, inoculate the agaric spores in a hundred-level sterile inoculation room, and the inoculation amount is calculated as 6-9 grams of spores per bag. After inoculation, the cultivation bag is transferred to a culture room with temperature controlled at 22-26°C, humidity maintained at 65%-75%, and light intensity of 300-600 lx for cultivation, and the cultivation period is about 70-100 days. The results show that the use of this substrate for cultivating agaric has a slower mycelium germination speed, and the mycelium full-bag time is 38-45 days. During the growth of agaric, the ear piece diameter is 4-6 cm on average, the ear piece is relatively thin, the edge flatness is poor, and the color is slightly lighter, and the yield of agaric per bag is 0.8-1.0 kg.

[0096] 2) Treatment of waste edible fungus substrate: After the agaric is harvested, the waste edible fungus substrate is immediately collected and stacked into a standard compost pile with a width of about 1.5 meters and a height of about 1 meter. The automatic turning machine is used to turn the pile every 4 days. After 40 days of fermentation treatment, the substrate is also rotten to dark brown, and the texture is loose and porous, but the smell is slightly heavy during the rotting process, and the fermentation process is not sufficient.

[0097] 3) Preparation of nursery substrate for horticultural plants: the composted substrate is processed by high-efficiency crushing and fine screening equipment to obtain the nursery substrate for horticultural plants. Add 18% perlite and 5% vermiculite to the nursery substrate, mix thoroughly, and use for the nursery of irises. The nursery environment maintains a temperature of 20-25°C, a substrate humidity of 50%-60%, an air humidity of 60%-70%, and a daily light duration of 8-10 hours with a light intensity of 2000-3000 lx. The results show that the germination rate of iris seeds is 80%-85%, the seedling growth is relatively not uniform, and part of the seedlings are slightly weak with light green leaves.

[0098] In the above comparative example, only 80% selected wood chips and 20% dried straw are used, lacking the nutritional and structural optimization effect of waste old bee guts. In Example 4, 30% of the pretreated waste old bee gut particles are mixed with 50% selected wood chips and 20% dried straw, adding unique nutritional ingredients to the substrate by introducing waste old bee guts, improving the substrate structure, and enhancing the air permeability and water retention. Compared with the two methods, the substrate of Example 4 is more nutrient-rich and has a better structure, providing better conditions for the growth of agaric.

[0099] In the above comparative example, the mycelium germination is slow, the bag-filling time is 38-45 days, the ear diameter is 4-6 cm, the edge flatness is poor, and the color is slightly lighter, with a yield of 0.8-1.0 kg per bag. In Example 4, the substrate with added waste old bee guts has fast mycelium germination, with a bag-filling time of 30-35 days, large and thick ears, a diameter of 6-8 cm, a neat edge, and a black and shiny color, with a yield of 1.0-1.3 kg per bag. Compared with the two methods, Example 4 is significantly better than Comparative Example 4 in terms of agaric growth speed, quality, and yield, indicating that waste old bee guts effectively promote the growth and development of agaric.

[0100] In the above comparative example, the waste old substrate is stacked into a compost pile with a width of about 1.5 meters and a height of about 1 meter. After 40 days of fermentation, it is also composted to dark brown and loose texture, but the smell is slightly heavy and the fermentation is not sufficient. In Example 4, after the agaric is harvested, the waste old substrate is stacked into a compost pile with a width of about 1.5 meters and a height of about 1 meter. After 40 days of fermentation, it is composted to dark brown and loose texture without a pungent odor, thanks to the optimization of substrate structure and composition by waste old bee guts, and the fermentation is fully stable.

[0101] In the above comparative example, the composted substrate is added with 18% perlite and 5% vermiculite for iris seedling cultivation. Under specific temperature, humidity, and light conditions, the seed germination rate is 80%-85%, the seedling growth is not uniform, part of the seedlings are weak, and the leaves are light green. In Example 4, under the same treatment and nursery environment, the seed germination rate is above 92%, the seedling growth is uniform and healthy, and the leaves are fresh green. Compared with the two methods, the iris seedling effect of Example 4 is better, reflecting the positive impact of the recycling and breeding substrate involving waste old bee guts on the nursery of horticultural plants.

[0102] The application changes the traditional single substrate utilization mode to a recycling mode, converting the agricultural waste of waste bee guts into a high-value resource. The waste bee guts are treated by artificial cleaning combined with hot water soaking to remove impurities, beeswax and propolis, ensuring the purity of subsequent processing. High temperature and high pressure sterilization technology is used to kill potential pathogens, insect eggs and other harmful organisms, providing a safe environment for the growth of edible fungi and garden plants. A specific composting and fermentation process is used to convert waste edible fungus substrate into high-quality garden plant seedling substrate, improving the utilization rate of waste bee guts and edible fungus substrate, improving the yield and quality of edible fungi, and the germination rate and survival rate of garden plants. At the same time, the application makes full use of the rich bioactive ingredients in waste bee guts, such as resin, oil, alkaloids, tannins, organic acids, amino acids, proteins, etc., on the basis of controlling temperature, humidity, light and other environmental conditions, balancing the needs of edible fungi and garden plants for growth environment (air humidity, temperature, shade), without using chemical additives during cultivation and without using high-cost imported substrates, under suitable conditions to promote the rapid growth of edible fungus mycelium and the germination of garden plant seeds, with an edible fungus yield growth rate of 10%-20% and a garden plant seed germination rate of more than 90%. The application prepares the breeding substrate through precise crushing and screening process, and meets the requirements of substrate breeding, effectively solving the technical problems of resource waste, environmental pollution, high substrate cost, poor water retention and air permeability, and unbalanced nutrient supply in the existing edible fungus cultivation and garden plant seedling method. Compared with the prior art, the application fully considers the recycling of resources in the substrate preparation process, uses the unique water retention and air permeability of waste bee guts and nutrient components, optimizes the substrate structure, improves the air permeability and water retention of the substrate, makes the nutrient components of the substrate more balanced, shortens the edible fungus mycelium filling time and the garden plant seedling cycle, reduces the early investment cost, and basically does not need a large amount of new resources, high energy consumption equipment and other additional inputs in the whole recycling process, with low comprehensive cost. The application has the advantages of high utilization rate of waste bee guts, waste edible fungus substrate and other agricultural wastes, simple preparation process, low production cost, good breeding effect, environmental protection and sustainability.

Claims

1. A method for preparing a double-circulation breeding matrix based on waste honeycombs, characterized in that: The following steps are involved: 1) Pretreatment of used honeycombs: Collect used honeycombs and remove surface impurities, residual beeswax, and propolis through a combination of manual cleaning and hot water soaking. After soaking, rinse thoroughly, dry, and crush into uniform particles. Finally, sterilize the particles in a high-temperature and high-pressure autoclave. 2) Prepare the edible fungus breeding matrix: Mix the pre-treated waste honeycomb pellets with conventional edible fungus matrix materials such as sawdust, cottonseed hulls, rice straw, and corn cobs, add an appropriate amount of water to control the matrix moisture content to 60%-70%, and stir evenly. The pre-treated waste honeycomb pellets should account for 20%-40% of the total mass of the edible fungus matrix materials. 3) Mushroom cultivation: After mixing evenly, place the mixture into mushroom cultivation bags or bottles. Perform conventional mushroom inoculation procedures under a sterile environment. Then, transfer the inoculated cultivation containers to a culture room with controlled temperature, humidity, and light conditions for cultivation. Finally, harvest the mushrooms according to the maturity standards. 4) Treatment of Waste Mushroom Substrates: After the mushrooms are harvested, the waste mushroom substrates are quickly collected and transported to a composting site. During the composting process, the compost is turned every 3-5 days to ensure that the internal and external materials are fully mixed and oxygen is exchanged. The fermentation time lasts for 30-45 days until the substrate is fully decomposed. 5) Preparation of horticultural plant seedling substrate: The decomposed waste edible fungus substrate from step 4) is pulverized using a pulverizing machine. Parameters are precisely controlled during the process to directly pulverize the substrate to a particle size that meets the horticultural plant seedling substrate standard. The substrate is then screened using a vibrating screen to effectively remove large impurities. Based on the substrate requirements of different horticultural plant species during the seed germination, cutting rooting, and seedling growth stages, mineral additives are scientifically added to the seedling substrate in a weight ratio to adjust the air permeability and water retention of the substrate to obtain the seedling substrate for plant seedling cultivation.

2. The method for preparing a double-circulation breeding matrix based on waste honeycombs according to claim 1, characterized in that: The manual cleaning described in step 1) is to gently brush the surface of the honeycomb with a brush to remove some loosely attached impurities, beeswax fragments and propolis particles.

3. The method for preparing a double-circulation breeding matrix based on waste honeycombs according to claim 1, characterized in that: In step 1), the temperature of hot water soaking is 85°C ± 2°C, and the soaking time is 45 minutes.

4. The method for preparing a double-circulation breeding matrix based on waste honeycombs according to claim 1, characterized in that: Step 1) Maintain the drying temperature at 35°C ± 2°C for 12-24 hours. During the drying process, turn the honeycomb every 2-3 hours to ensure uniform drying and achieve a moisture content of 12%-15%. The particle size after drying is 1-3 cm.

5. The method for preparing a double-circulation breeding matrix based on waste honeycombs according to claim 1, characterized in that: The conditions for high temperature and high pressure sterilization are: 120-130°C temperature range, 0.1-0.2MPa pressure range, and sterilization for 30-60 minutes.

6. The method for preparing a double-circulation breeding matrix based on waste honeycombs according to claim 1, characterized in that: The conventional edible fungus substrate raw materials in step 3) are sawdust, cottonseed hulls, rice straw or corn cobs.

7. The method for preparing a double-circulation breeding matrix based on waste honeycombs according to claim 1, characterized in that: In step 4), the criteria for judging whether the food is mature are that the color changes to dark brown, the texture becomes loose and porous, and there is no pungent odor.

8. The method for preparing a double-circulation breeding matrix based on waste honeycombs according to claim 1, characterized in that: In step 5), the mineral additive is perlite or vermiculite.