Copper peptide tea extract composition with anti-wrinkle and moisturizing effects and application thereof

Abstract

The present application relates to a kind of copper peptide tea extract composition with anti-wrinkle, moisturizing efficacy and application, belong to the field of cosmetics.The copper peptide tea extract composition of the present application includes the following weight parts of components: epigallocatechin gallate 0.001~0.2 parts, blue copper peptide 0.05~0.1 parts and camellia seed oil 0.5~5 parts.The copper peptide tea extract composition of the present application will EGCG, blue copper peptide, camellia seed oil three components in a certain range after compounding, has synergistic effect, can exert maximum skin care efficacy, and has strong anti-wrinkle, strong moisturizing efficacy when applied in cosmetics.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, and in particular to a copper peptide tea extract composition and its application that has anti-wrinkle and moisturizing effects. Background Technology

[0002] Epigallocatechin gallate (EGCG) is a natural polyphenolic compound extracted from tea leaves. Belonging to the catechin family, it is one of the main components of tea polyphenols. EGCG possesses strong antioxidant capabilities, effectively scavenging free radicals in the skin and reducing their damage to skin cells, thereby slowing down the skin aging process. It can promote collagen production, enhance skin elasticity, reduce wrinkles, and make the skin firmer and smoother. Furthermore, it exhibits various biological activities, including anti-inflammatory, anti-cancer, and cardiovascular protective effects, and has broad application prospects in the fields of medicine, health products, and cosmetics.

[0003] Copper tripeptide (Tripeptide-1 copper), hailed as the "ancestor of peptides," was discovered in the 1970s by American Dr. Loren Pickart. It was found to be highly effective in treating wounds and skin injuries, not only reducing scar tissue formation but also stimulating the skin's natural healing process. Therefore, it is far ahead in skin repair efficacy.

[0004] Camellia seed oil is a pure, natural, high-grade edible vegetable oil extracted from the seeds of plants in the Camellia genus of the Theaceae family. Its main components include unsaturated fatty acids, especially oleic acid and linoleic acid, with a content exceeding 90%. It also contains abundant vitamins A, B, D, and E, as well as essential linolenic acid. These components give camellia seed oil significant effects in balancing blood lipids, promoting cardiovascular health, beautifying skin and delaying aging, anti-tumor activity, and improving digestive function.

[0005] Copper peptides are limited in their application in a wide range of cosmetics due to their high cost. While EGCG has excellent skincare benefits, its poor stability limits its application concentration, preventing it from reaching its maximum effectiveness. Camellia seed oil, when used alone, does not provide significant anti-wrinkle effects. Summary of the Invention

[0006] The purpose of this invention is to overcome the shortcomings of the prior art and provide a copper peptide tea extract composition and its application with anti-wrinkle and moisturizing effects.

[0007] To achieve the above objectives, the technical solution adopted by the present invention is as follows:

[0008] In a first aspect, the present invention provides a copper peptide tea extract composition comprising the following components in parts by weight: 0.001 to 0.2 parts of epigallocatechin gallate, 0.05 to 0.1 parts of blue copper peptide, and 0.5 to 5 parts of camellia seed oil.

[0009] This invention combines EGCG, copper peptides, and camellia seed oil in specific proportions to create a copper peptide tea extract composition that has a synergistic effect and strong anti-wrinkle and moisturizing properties.

[0010] As a preferred embodiment of the copper peptide tea extract composition of the present invention, the copper peptide tea extract composition comprises the following components in parts by weight: 0.1 to 0.2 parts of epigallocatechin gallate, 0.05 to 0.1 parts of blue copper peptide, and 1 to 5 parts of camellia seed oil.

[0011] More preferably, the copper peptide tea extract composition comprises the following components in parts by weight: 0.2 parts epigallocatechin gallate, 0.1 parts blue copper peptide, and 5 parts camellia seed oil.

[0012] Secondly, this invention provides the application of the copper peptide tea extract composition in the preparation of moisturizing and / or anti-wrinkle products. The moisturizing curve of the copper peptide tea extract composition is higher than that of the positive control, indicating that the copper peptide tea extract composition has moisturizing effects; and it significantly inhibits elastase, i.e., it has good anti-wrinkle effects.

[0013] Thirdly, the present invention provides a cosmetic product containing the copper peptide tea extract composition described above.

[0014] Furthermore, the cosmetic contains 0.551 to 5.30 parts of the copper peptide tea extract composition and 94.7 to 99.449 parts of cosmetic excipients.

[0015] As a preferred embodiment of the copper peptide tea extract composition of the present invention, the cosmetic contains 5.30 parts of the copper peptide tea extract composition and 94.7 parts of cosmetic excipients.

[0016] Furthermore, the dosage form of the cosmetic includes at least one of water, lotion, and cream.

[0017] Furthermore, the cosmetic excipients include at least one of emollients, dispersants, humectants, preservatives, emulsifying stabilizers, thickeners, and pH adjusters.

[0018] Further, in the cosmetic excipients, the emollient includes at least one of caprylic / capric triglyceride, shea butter, cetyl ethylhexanoate, and polydimethylsiloxane; the moisturizer includes at least one of allantoin, glycerin, tocopheryl acetate, and butylene glycol; the preservative includes p-hydroxyacetophenone and / or phenoxyethanol / ethylhexylglycerin; the emulsifying stabilizer includes at least one of cetearyl alcohol, cetyl / glyceryl stearate / PEG-75 stearate, and glyceryl stearate / PEG-100 stearate; the thickener includes at least one of carbomer, xanthan gum, and polyacrylamide / C13-14 isoparaffin; and the pH adjuster includes aminomethylpropanol.

[0019] Because EGCG and copper peptides in the copper peptide tea extract composition are highly susceptible to oxidation, and tocopheryl acetate has strong antioxidant properties, the addition of tocopheryl acetate as an excipient can protect and stabilize EGCG and copper peptides. Tocopheryl acetate can also act as a solubilizer, aiding in the dispersion of the copper peptide tea extract composition.

[0020] As a preferred embodiment of the copper peptide tea extract composition of the present invention, the cosmetic excipients comprise the following components in parts by weight: 72.63 parts water, 0.15 parts carbomer, 0.05 parts allantoin, 0.08 parts xanthan gum, 4 parts glycerin, 4 parts caprylic / capric triglyceride, 0.8 parts cetearyl alcohol, 2 parts cetyl ethylhexanoate, 0.5 parts tocopheryl acetate, 2 parts cetyl / glyceryl stearate / PEG-75 stearate, 1 part glyceryl stearate / PEG-100 stearate, 1 part shea butter, 1 part polydimethylsiloxane, 0.45 parts polyacrylamide / C13-14 isoparaffin, 3 parts butylene glycol, 0.5 parts p-hydroxyacetophenone, 0.04 parts aminomethylpropanol, and 0.5 parts phenoxyethanol / ethylhexylglycerin.

[0021] Furthermore, the dosage form of the cosmetic includes at least one of water, lotion, and cream.

[0022] Fourthly, the present invention provides a method for preparing the cosmetic, comprising the following steps:

[0023] S1: Mix carbomer, allantoin, xanthan gum, glycerin and water, homogenize at 2000-2500 rpm for 1-3 min, stir at 800-1200 rpm, adjust the temperature to 80-85℃, homogenize at 2000-2500 rpm for 1-3 min, keep warm and stir at 800-1200 rpm for 10-15 min, and stir until evenly dissolved;

[0024] S2: Add the B phase raw material to the mixture processed in step S1, homogenize at 800-1200 rpm for 5-8 min, and stir evenly at 800-1200 rpm;

[0025] S3: Adjust the temperature of the product processed in step S2 to 50-55℃, add polyacrylamide / C13-14 isoparaffin and D phase raw material, and stir at 800-1200 rpm for 3-8 minutes until the mixture is uniform.

[0026] S4: Adjust the temperature of the product processed in step S3 to 40-45℃, add the E phase raw material and phenoxyethanol / ethylhexylglycerin, and stir evenly at 800-1200 rpm.

[0027] S5: Add the G phase raw material to the product processed in step S4, stir evenly at 600-1000 rpm, adjust the temperature to 30-38℃, filter, and the cosmetic product is obtained.

[0028] The A-phase raw material includes at least one of carbomer, allantoin, xanthan gum, glycerin, and water;

[0029] The B-phase raw materials include at least one of the following: caprylic / capric triglyceride, camellia seed oil, cetearyl alcohol, cetyl ethylhexanoate, tocopheryl acetate, cetyl alcohol / glyceryl stearate / PEG-75 stearate, glyceryl stearate / PEG-100 stearate, shea butter, and polydimethylsiloxane.

[0030] The C-phase raw material includes polyacrylamide / C13-14 isoparaffins;

[0031] The D-phase raw material includes butanediol and / or p-hydroxyacetophenone;

[0032] The E-phase raw material includes aminomethylpropanol and / or water;

[0033] The F-phase raw material includes phenoxyethanol / ethylhexylglycerin and / or water;

[0034] The G-phase raw materials include epigallocatechin gallate and blue copper peptide.

[0035] Further, in step S2, the components of phase B raw material are mixed, the temperature is adjusted to 80-85°C, stirred and dissolved, and then added to the mixture processed in step S1.

[0036] Further, in step S3, the components of the D phase raw material are mixed, the temperature is adjusted to 55-60°C, stirred to dissolve, and then added, preferably at 60°C.

[0037] Preferably, in step S4, the temperature of the product processed in step S3 is adjusted to 45°C.

[0038] Preferably, in step S5, the temperature is adjusted to 38°C.

[0039] Further, in step S5, the components of the G phase raw material are mixed, dispersed evenly, and then added.

[0040] Furthermore, in step S5, the filter is 80-100 mesh, preferably 100 mesh.

[0041] Furthermore, step S5 also includes inspecting, filling, and packaging the filtered cosmetics to obtain the final commercially available cosmetic product.

[0042] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0043] The high cost of copper peptides limits their widespread use in cosmetics. While EGCG has excellent skincare benefits, its poor stability limits its application concentration, preventing it from reaching its maximum potential. Camellia seed oil, when used alone, does not provide significant anti-wrinkle effects. This invention combines EGCG, copper peptides, and camellia seed oil within a certain range, resulting in a synergistic effect that maximizes skincare efficacy. When applied to cosmetics, it offers strong anti-wrinkle and moisturizing benefits, opening up new possibilities for cosmetic applications. Attached Figure Description

[0044] Figure 1 The moisturizing-time curve of the copper peptide tea extract composition. Detailed Implementation

[0045] To better illustrate the purpose, technical solution, and advantages of this invention, the invention will be further described below with reference to specific embodiments. Unless otherwise specified, other materials and reagents used in the embodiments are commercially available.

[0046] Epigallocatechin gallate (EGCG), purchased from Xiyuan Biotechnology Co., Ltd.

[0047] Blue copper peptide, purchased from Viki (Guangzhou) Technology Co., Ltd.

[0048] Camellia seed oil, purchased from Guangzhou Boteng Trading Co., Ltd.

[0049] Acetyl hexapeptide-8 was purchased from Zhejiang Paipai Biotechnology Co., Ltd.

[0050] Shea butter (BUTYROSPERMUM PARKII), purchased from Ahuskars Oils Ltd.

[0051] Examples 1-12

[0052] The components of the copper peptide tea extract compositions described in Examples 1 to 12 are shown in Table 1.

[0053] Table 1. Composition (parts by weight) of the copper peptide tea extract compositions described in Examples 1-12

[0054]

[0055] The components of the copper peptide tea extract compositions described in Comparative Examples 1 to 12 are shown in Table 2.

[0056] Table 2. Composition (parts by weight) of the copper peptide tea extract compositions described in Comparative Examples 1-12

[0057]

[0058] Among them, Table 2 " / "" indicates that the corresponding copper peptide tea extract composition components were not added. The only differences between Comparative Examples 1-12 and Example 9 are: Comparative Example 1 did not add EGCG; Comparative Example 2 did not add blue copper peptide; Comparative Example 3 did not add camellia seed oil; Comparative Example 4 did not add copper peptide tea extract composition; the content of each component of the copper peptide tea extract composition in Comparative Example 5 was twice that of Example 9; Comparative Examples 6, 7, and 8 added the optimal weight parts of EGCG, blue copper peptide, and camellia seed oil separately; Comparative Examples 9, 10, and 11 added EGCG, blue copper peptide, and camellia seed oil separately to the optimal weight parts of the copper peptide tea extract composition; Comparative Example 12 replaced blue copper peptide with an equal amount of acetyl hexapeptide-8. Missing components were replaced with an equal weight part of water.

[0059] Application Examples 1-12:

[0060] Application examples of the cosmetic described in this invention; the cosmetic is specifically a face cream; the cosmetic includes a copper peptide tea extract composition. The mass percentage of the copper peptide tea extract composition in the cosmetic in each application example is shown in Table 3.

[0061] Table 3 shows the content of copper peptide tea extract compositions added in each application example.

[0062]

[0063] The formula of the cosmetic is shown in Table 4.

[0064] Table 4. Cosmetic Formulas

[0065]

[0066]

[0067] The preparation method of the cosmetic includes the following steps:

[0068] Preprocessing:

[0069] (1) Mix caprylic / capric triglyceride, camellia seed oil, cetearyl alcohol, cetyl ethylhexanoate, tocopheryl acetate, cetyl alcohol / glyceryl stearate / PEG-75 stearate, glyceryl stearate / PEG-100 stearate, shea butter and polydimethylsiloxane in sequence, stir and heat to 80-85°C, stir until completely dissolved, and set aside as raw material for pretreatment phase B.

[0070] (2) Heat butanediol and p-hydroxyacetophenone to 60°C, stir and dissolve evenly, and set aside as raw material for pretreatment of phase D.

[0071] (3) Add a small amount of water to aminomethylpropanol and stir until homogeneous. Set aside as raw material for pretreatment of E phase.

[0072] (4) Add a small amount of water to phenoxyethanol / ethylhexylglycerin and stir until homogeneous. Set aside as raw material for pretreatment of phase F.

[0073] (5) EGCG and copper peptides are pre-dispersed evenly and used as raw materials for pretreatment of phase G.

[0074] Process standards:

[0075] (1) Add phase A raw materials (carbomer, allantoin, xanthan gum, glycerin and water) to an emulsifying pot, homogenize at 2000-2500 rpm for 1-3 min, stir at 800-1200 rpm, heat to 80-85℃, homogenize at 2000-2500 rpm for 1-3 min, keep warm and stir at 800-1200 rpm for 10-15 min, and stir until dissolved evenly.

[0076] (2) Add the pretreated B phase raw material to the mixture treated in step (1), homogenize at 800-1200 rpm for 5-8 min, and stir evenly at 800-1200 rpm.

[0077] (3) Cool the product treated in step (2) to 50-55°C, add the C phase raw material (polyacrylamide / C13-14 isoparaffin) and the pretreated D phase raw material in sequence, and stir at 800-1200 rpm for 3-8 minutes until the mixture is uniform.

[0078] (4) Cool the product processed in step (3) to 45°C, add the pretreated E phase and pretreated F phase raw materials in sequence, and stir evenly at 800-1200 rpm.

[0079] (5) Add the pretreated G phase raw material to the product processed in step (4) in small amounts and multiple times, and stir evenly at 600-1000 rpm.

[0080] (6) Cool the product processed in step (5) to 38°C, take a sample, and control it in the central region.

[0081] (7) After the central control of step (6) is qualified, the material is discharged through a 100-mesh filter.

[0082] (8) After the product is discharged in step (7), let it stand and then inspect the semi-finished product.

[0083] (9) After the semi-finished product in step (8) passes the inspection, it is filled and packaged.

[0084] (10) The product from step (9) is inspected as a finished product. After passing the inspection, it is put into storage.

[0085] Application Comparative Examples 1-5:

[0086] The only difference between Comparative Example 1 and Example 9 is that EGCG is replaced with an equal amount of water.

[0087] The only difference between Comparative Example 2 and Application Example 9 is that the blue copper peptide is replaced with an equal amount of water.

[0088] The only difference between Comparative Example 3 and Example 9 is that camellia seed oil is replaced with an equal amount of water.

[0089] The only difference between Comparative Example 4 and Application Example 9 is that the copper peptide tea extract composition is replaced with an equal amount of water.

[0090] The only difference between Comparative Example 5 and Example 9 is that the content of each component in the copper peptide tea extract composition is twice that of Example 9.

[0091] The only difference between Comparative Example 6 and Application Example 9 is that the copper peptide and camellia seed oil are replaced with an equal amount of water.

[0092] The only difference between Comparative Example 7 and Application Example 9 is that EGCG and camellia seed oil are replaced with an equal amount of water.

[0093] The only difference between Comparative Example 8 and Application Example 9 is that EGCG and copper peptides are replaced with an equal amount of water.

[0094] The only difference between Comparative Example 9 and Application Example 9 is that 5.3 parts of the copper peptide tea extract composition were replaced with an equal amount of EGCG.

[0095] The only difference between Comparative Example 10 and Application Example 9 is that 5.3 parts of copper peptide tea extract composition were replaced with an equal amount of blue copper peptide.

[0096] The only difference between Comparative Example 11 and Application Example 9 is that 5.3 parts of the copper peptide tea extract composition were replaced with an equal amount of camellia seed oil.

[0097] The only difference between Comparative Example 12 and Application Example 9 is that 0.1 parts of blue copper peptide were replaced with an equal amount of acetyl hexapeptide-8.

[0098] The preparation method of the cosmetic is the same as that in the application examples.

[0099] Test Example 1: Determination of Physicochemical Indicators

[0100] 1. Test samples: Three batches of face cream prepared using Example 9.

[0101] 2. Measure the conductivity color using a conductivity meter; pour the test sample into a transparent sample bottle and observe the color of the composition, then measure the refractive index using a refractometer.

[0102] 3. The test results are shown in Table 5. The results show that the physicochemical and appearance indicators of the copper peptide tea extract composition of Example 9 of the present invention meet the standards in actual production.

[0103] Table 5

[0104] sample electrical conductivity color Batch 1 147 light blue Batch 2 152 light blue Batch 3 141 light blue

[0105] Test Example 2: Safety Patch Test

[0106] 1. Test samples: face creams prepared using Examples 1-12 and Comparative Examples 1-12.

[0107] 2. Subjects: 9 males and 26 females, a total of 35 subjects, aged 22 to 45 years, with an average age of 28.1 ± 5.6 years, meeting the subject voluntary inclusion criteria.

[0108] 3. The spot test method is as follows:

[0109] Select qualified patch testing equipment and use the closed patch test method to apply the patch containing the test sample (cut to the size of the patch tester, approximately 50mm). 2 The test sample was placed in a patch applicator and applied to the back of the subject with hypoallergenic adhesive tape. After 24 hours, the test sample was removed. Skin reactions were observed at 0.5, 24, and 48 hours after removal of the patch containing the test sample. A negative control (blank, no treatment) was also provided. The results were recorded according to the grading standards for skin reactions in occlusive patch tests in Chapter 7 of the "Cosmetic Safety Technical Specifications" (2015 edition).

[0110] 4. Safety patch test results are shown in Table 6. The results of the human skin patch test showed that among the 35 subjects, 0 cases of adverse skin reactions occurred, indicating that the copper peptide tea extract composition prepared in this invention has high safety.

[0111] Table 6

[0112]

[0113]

[0114] Test Example 3: Moisturizing Curve Test

[0115] 1. Experimental Principle: An ideal moisturizing product should have the ability to retain moisture; that is, the smaller the change in moisture content, the stronger the moisturizing ability. This experimental method continuously monitors the change in moisture weight of the treated test samples and plots a moisturizing rate-time curve. The moisturizing ability of the test samples is observed based on the trend of the moisturizing rate curve, thereby evaluating the skin moisturizing efficacy of the test samples.

[0116] 2. Judgment criteria: The sample is judged to have moisturizing effect by comparing whether the moisturizing rate curve of the test sample is higher (better) than that of the positive control moisturizing rate curve.

[0117] 3. Test methods:

[0118] (1) Weighing the sample.

[0119] (2) Pre-treat the test samples and select a reagent with known hygroscopic ability as a positive control (the positive control reagent in this test is glycerol).

[0120] (3) Place the test samples and positive controls in a constant temperature and humidity equipment under certain temperature and humidity conditions.

[0121] (4) Weigh the test samples regularly.

[0122] (5) Record the data and calculate the moisture retention rate to create a curve.

[0123] 4. The test results are shown in Table 7 and Figure 1 As shown in the test results, the moisturizing curve of the test sample, namely the copper peptide tea extract composition, is higher than (better than) the moisturizing rate curve of the positive control, indicating that the copper peptide tea extract composition has moisturizing effects, that is, it has good anti-wrinkle effects. Among them, the copper peptide tea extract composition of Example 9 has the best effect, that is, the optimal weight ratio of the copper peptide tea extract composition is 0.2 parts of EGCG, 0.1 parts of blue copper peptide, and 5 parts of camellia seed oil. After replacing the blue copper peptide with acetyl hexapeptide-8 in Comparative Example 12, the moisturizing rate after 1.5 hours is only 84.4%, which is lower than that of Example 9.

[0124] Table 7

[0125]

[0126]

[0127] Test Example 4: Elastase Test

[0128] 1. Experimental Principle: Collagen forms collagen fibers and reticular fibers, while elastin forms elastic fibers. These three types of fibers together constitute the connective tissue of the skin, creating a plump and elastic structure. Elastase has the ability to break down various proteins, including collagen and elastin. Changes in elastin structure are closely related to skin aging. An enzyme-linked immunosorbent assay (ELISA) reader is used to detect the inhibitory effect of the test sample on elastase-catalyzed substrates, determining whether the test sample has the ability to inhibit elastase.

[0129] 2. Reagents and materials: 30 U / mg elastase (pig trypsin), N-succinyl-alanine-alanine-p-nitroaniline, tris(hydroxymethyl)aminomethane (Tris), concentrated hydrochloric acid and EGCG.

[0130] 3. Instruments and equipment: analytical balance (accurate to 0.001g), ultrasonic cleaner, pipette, pipette, colorimetric tubes and volumetric flasks (10mL and 100mL).

[0131] 4. Measurement steps:

[0132] (1) Preparation of solution:

[0133] ① Prepare a 0.2 mol / L Tris-HCl buffer solution (pH 8.0):

[0134] Solution A: Accurately weigh 4.8456 g of Tris particles, add 100 mL of deionized water, stir and mix to dissolve, and obtain a 0.4 mol / L Tris solution;

[0135] Solution B: Take 3.34 mL of concentrated hydrochloric acid and dilute it with 96.66 mL of deionized water to obtain a 0.4 mol / L HCl solution;

[0136] Mix 100 mL of solution A and 44.76 mL of solution B, adjust the pH to 8.0, and add deionized water to bring the volume to 200 mL.

[0137] ② Elastase solution (enzyme solution): Prepared with buffer solution. Take 0.033g of elastase, add 10mL of buffer solution to prepare a 100U / mL elastase solution. Prepare immediately before use.

[0138] ③ Substrate solution: Weigh 1.8057 mg of N-succinyl-alanine-alanine-alanine-p-nitroaniline and dissolve it in 10 mL of buffer solution to obtain a 0.18057 mg / mL N-succinyl-alanine-alanine-alanine-p-nitroaniline solution.

[0139] ④ Positive control EGCG solution: Weigh 0.001g of EGCG powder, dissolve it in 1mL of buffer solution to prepare a 1.0mg / mL EGCG solution, and then dilute it with buffer solution to prepare 0.5, 0.1, 0.01, 0.005, 0.003, 0.002 and 0.001mg / mL EGCG solutions.

[0140] ⑤ Sample solution: copper peptide tea extract composition solutions of Examples 1-12 and Comparative Examples 1-12 (diluted with buffer).

[0141] (2) Prepare the test solution:

[0142] Prepare the test solution according to Table 8.

[0143] Table 8

[0144]

[0145]

[0146] (3) Add each solution to a 96-well plate in the order shown in Table 8, incubate at room temperature for 60 min, detect absorbance at 410 nm using an ELISA reader, record and save the data, and calculate the elastase inhibition rate (%) according to formula (Ⅰ).

[0147] Formula (I): Elastase inhibition rate (%) = [1-(OD)] A -OD B ) / (OD C -OD D In the above formula, OD is calculated as follows: OD = 100% × 100% A The absorbance values ​​are for the sample group (containing enzyme and substrate).

[0148] OD B The absorbance value is for the control group (containing enzyme but not substrate);

[0149] OD C The absorbance value is for the blank group (containing enzyme and substrate but no sample).

[0150] OD D The absorbance value is for the blank control group (containing enzyme but no substrate and no sample).

[0151] 5. Precision: The absolute difference between two independent measurements obtained under repeatability conditions shall not exceed 10% of the arithmetic mean.

[0152] Table 9

[0153]

[0154]

[0155] The test results are shown in Table 9. The copper peptide tea extract composition significantly inhibits elastase, indicating good anti-wrinkle effects. The copper peptide tea extract composition of Example 9 showed the best effect; the optimal ratio of the copper peptide tea extract composition was 0.2 parts EGCG, 0.1 parts blue copper peptide, and 5 parts camellia seed oil. In Comparative Example 12, after replacing the blue copper peptide with acetyl hexapeptide-8, the elastase inhibition rate was only 40.35 ± 1.86%, lower than that of Example 9.

[0156] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A cosmetic product, characterized by, The cosmetic contains 5.30 parts of the copper peptide tea extract composition and 94.7 parts of cosmetic adjuvant; The copper peptide tea extract composition comprises the following components by weight: 0.2 parts of epigallocatechin gallate, 0.1 parts of blue copper peptide and 5 parts of camellia seed oil; The cosmetic adjuvant is selected from emollients, dispersants, humectants, preservatives, emulsion stabilizers, thickening agents and pH regulators; The emollients are selected from caprylic / capric triglyceride, shea butter, cetyl alcohol ethylhexanoate and dimethicone; the humectants are selected from allantoin, glycerin, tocopheryl acetate and butylene glycol; the preservatives are selected from p-hydroxyacetophenone and / or phenoxyethanol / ethylhexylglycerin; the emulsion stabilizers are selected from cetearyl alcohol, cetyl alcohol / glyceryl stearate / PEG-75 stearate and glyceryl stearate / PEG-100 stearate; the thickening agents are selected from carbomer, xanthan gum and polyacrylamide / C13-14 isoparaffin; and the pH regulators are selected from aminomethyl propanol.

2. The cosmetic according to claim 1, wherein The dosage form of the cosmetic is at least one of water, milk and cream.

3. The cosmetic product according to claim 1, wherein The cosmetic is a moisturizing and / or anti-wrinkle product.

4. The method for preparing a cosmetic product according to any one of claims 1 to 3, characterized in that, The method comprises the following steps: Pre-treatment: (1) Mix caprylic / capric triglyceride, camellia seed oil, cetearyl alcohol, cetyl alcohol ethylhexanoate, tocopheryl acetate, cetyl alcohol / glyceryl stearate / PEG-75 stearate, glyceryl stearate / PEG-100 stearate, shea butter and dimethicone in sequence, stir and heat to 80-85℃, and stir until completely dissolved, and reserve as pre-treatment B-phase raw material; (2) Heat butylene glycol and p-hydroxyacetophenone to 60℃, stir and dissolve uniformly, and reserve as pre-treatment D-phase raw material; (3) Stir a small amount of water in aminomethyl propanol until uniform, and reserve as pre-treatment E-phase raw material; (4) Stir a small amount of water in phenoxyethanol / ethylhexylglycerin until uniform, and reserve as pre-treatment F-phase raw material; (5) Disperse EGCG and blue copper peptide uniformly in advance, and reserve as pre-treatment G-phase raw material; The preparation method of the cosmetic is as follows: S1: Mix A-phase raw material, homogenize at 2000-2500 rpm for 1-3 min, stir at 800-1200 rpm, adjust the temperature to 80-85℃, homogenize at 2000-2500 rpm for 1-3 min, and stir at 800-1200 rpm for 10-15 min; S2: Add pre-treatment B-phase raw material to the mixture treated in step S1, homogenize at 800-1200 rpm for 5-8 min, and stir uniformly at 800-1200 rpm; S3: Adjust the temperature of the product treated in step S2 to 50-55℃, add polyacrylamide / C13-14 isoparaffin and pre-treatment D-phase raw material, and stir at 800-1200 rpm for 3-8 min until uniform; S4: Adjust the temperature of the product treated in step S3 to 45℃, add pre-treatment E-phase raw material and pre-treatment F-phase raw material, and stir uniformly at 800-1200 rpm; S5: in the product treated in step S4, the pre-processed G-phase raw material is added, stirred uniformly at 600-1000 rpm, the temperature is adjusted to 38°C, and then filtered, thus obtaining the cosmetic product; The A-phase raw material is selected from carbomer, allantoin, xanthan gum, glycerol and water.

Images