Gamma-polyglutamic acid / zein composite nanoparticles as well as preparation method and application thereof

Abstract

The invention discloses gamma-polyglutamic acid / zein composite nanoparticles as well as a preparation method and application thereof, and the preparation method comprises the following steps: dissolving zein in an ethanol solution to obtain a zein solution; the preparation method comprises the following steps: dissolving gamma-polyglutamic acid in deionized water to obtain a gamma-polyglutamic acid solution; the zein solution is added into the gamma-polyglutamic acid solution, ethanol in the solution is removed through evaporation, insoluble substances are removed through centrifugation, and gamma-polyglutamic acid / zein composite nanoparticle dispersion liquid is obtained; and performing freeze drying to obtain gamma-polyglutamic acid / zein composite nanoparticle powder. The preparation method of the gamma-polyglutamic acid / zein composite nano-particles is simple, low in cost, green and safe, and the obtained nano-particles can be kept stable in a wide pH, ionic strength and temperature range, have good biocompatibility, can be applied to efficient embedding of bioactive components, and can be used for preparing the gamma-polyglutamic acid / zein composite nano-particles. The activity and the stability are improved.

Description

Technical Field

[0001] The invention belongs to the technical field of food, medicine and cosmetics, and specifically relates to a gamma-polyglutamic acid / zein composite nanoparticle and a preparation method and application thereof. Background Art

[0002] Bioactive ingredients are widely found in plants, animals or microorganisms, and have multiple functions such as antioxidant, anti-inflammatory, antihypertensive and anti-tumor, so they have attracted much attention in many application fields. However, most of these ingredients have poor water solubility and instability, and are prone to lose activity during storage, which limits their application. In order to improve their stability and water solubility, encapsulation technology has made significant progress as a practical strategy. In addition to protecting and enhancing the solubility of bioactive ingredients, encapsulation also supports targeted delivery. Among the common encapsulation carriers, nanoparticles are widely used due to their small size, high encapsulation efficiency, slow release rate and effective penetration ability, especially in the fields of food, medicine and cosmetics, showing unique advantages.

[0003] Zein is an amphipathic protein isolated from corn. It has the ability to self-assemble in the anti-solvent process. Nanoparticles prepared from it are a commonly used carrier. However, its hydrophobicity and instability under high ionic strength, neutral pH and heat treatment environments limit its application in food, medicine and cosmetics. To overcome these limitations, water-soluble proteins or polysaccharides are usually used for surface coating.

[0004] Nevertheless, achieving stability over a wide range of pH, ionic strength, and temperature remains a major challenge.

[0005] At present, there are no reports on the use of zein and γ-polyglutamic acid to prepare composite nanoparticles and use them as carriers of bioactive ingredients. Summary of the invention

[0006] The purpose of this section is to summarize some aspects of embodiments of the present invention and briefly introduce some preferred embodiments. Some simplifications or omissions may be made in this section and the specification abstract and the invention title of this application to avoid blurring the purpose of this section, the specification abstract and the invention title, and such simplifications or omissions cannot be used to limit the scope of the present invention.

[0007] In view of the above problems and / or the problems existing in the prior art, the present invention is proposed.

[0008] Therefore, the purpose of the present invention is to overcome the deficiencies in the prior art and provide a method for preparing γ-polyglutamic acid / zein composite nanoparticles.

[0009] In order to solve the above technical problems, the present invention provides the following technical solution: a method for preparing γ-polyglutamic acid / zein composite nanoparticles, comprising:

[0010] dissolving zein in an ethanol solution to obtain a zein solution;

[0011] dissolving γ-polyglutamic acid in deionized water to obtain a γ-polyglutamic acid solution;

[0012] The zein solution is added to the γ-polyglutamic acid solution, ethanol in the solution is evaporated away, and insoluble matter is removed by centrifugation to obtain a γ-polyglutamic acid / zein composite nanoparticle dispersion;

[0013] The γ-polyglutamic acid / zein composite nanoparticle powder was obtained by freeze drying.

[0014] As a preferred embodiment of the preparation method of the present invention, the concentration of the γ-polyglutamic acid solution is 0.01-2 wt %.

[0015] As a preferred embodiment of the preparation method of the present invention, the concentration of the zein solution is 0.1-10 wt %.

[0016] As a preferred embodiment of the preparation method of the present invention, the molecular weight of the γ-polyglutamic acid is 300,000 to 2,000,000 g / mol.

[0017] As a preferred embodiment of the preparation method of the present invention, the zein solution is added into the γ-polyglutamic acid solution, wherein the mass ratio of γ-polyglutamic acid to zein is 1:40 to 2:1.

[0018] As a preferred embodiment of the preparation method of the present invention, the pH of the γ-polyglutamic acid solution is 3-6.

[0019] Another object of the present invention is to overcome the deficiencies in the prior art and provide a method for preparing γ-polyglutamic acid / zein composite nanoparticles loaded with bioactive ingredients, comprising:

[0020] dissolving zein in an ethanol solution to obtain a zein solution, and dissolving a bioactive component in the zein solution to obtain a zein solution containing the bioactive component;

[0021] dissolving γ-polyglutamic acid in deionized water to obtain a γ-polyglutamic acid solution;

[0022] Adding a zein solution containing bioactive ingredients into a γ-polyglutamic acid solution, evaporating to remove ethanol in the solution, and centrifuging to remove insoluble matter to obtain a γ-polyglutamic acid / zein composite nanoparticle dispersion loaded with bioactive ingredients;

[0023] The γ-polyglutamic acid / zein composite nanoparticle powder loaded with bioactive components is obtained by freeze drying.

[0024] As a preferred embodiment of the method for preparing the bioactive component-loaded γ-polyglutamic acid / zein composite nanoparticles of the present invention, the mass ratio of the bioactive component to zein is 1:50 to 1:2.

[0025] As a preferred embodiment of the method for preparing the γ-polyglutamic acid / zein composite nanoparticles loaded with bioactive ingredients of the present invention, the bioactive ingredients include curcumin, catechin, EGCG, quercetin and baicalin.

[0026] Another object of the present invention is to overcome the deficiencies in the prior art and provide a method for using γ-polyglutamic acid / zein composite nanoparticles loaded with bioactive ingredients in the preparation of food, cosmetics and medicines.

[0027] Beneficial effects of the present invention:

[0028] (1) The present invention proposes for the first time a method for preparing γ-polyglutamic acid / zein composite nanoparticles, which is simple to operate, low in cost and easy to mass produce.

[0029] (2) The γ-polyglutamic acid / zein composite nanoparticles prepared by the present invention have excellent stability and can remain stable in a wider range of pH, ionic strength and temperature compared to single alcohol-soluble zein nanoparticles.

[0030] (3) The γ-polyglutamic acid / zein composite nanoparticles prepared by the present invention have good biocompatibility and can efficiently encapsulate bioactive ingredients. Compared with free or single zein nanoparticles, the bioactive ingredients in the composite nanoparticles have improved stability and activity. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following briefly introduces the drawings required for describing the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative labor. Among them:

[0032] Figure 1 The pH stability diagram of the zein nanoparticles and composite nanoparticles of the present invention.

[0033] Figure 2 Graph showing the ionic stability of the composite nanoparticles of the present invention.

[0034] Figure 3 This is a graph showing the ionic stability of the zein nanoparticles of the present invention.

[0035] Figure 4 This is a graph showing the thermal stability of the composite nanoparticles under different pH conditions of the present invention.

[0036] Figure 5 This is a graph showing the storage stability of the composite nanoparticles under different pH conditions of the present invention.

[0037] Figure 6 The scanning electron microscope pictures of the composite nanoparticles with and without curcumin embedded in the present invention are shown.

[0038] Figure 7 The cell compatibility diagram of the composite nanoparticles of the present invention.

[0039] Figure 8 Graph showing the thermal stability of free and embedded curcumin of the present invention.

[0040] Fig. 9 This is a graph of DPPH free radical scavenging rate of the present invention.

[0041] Fig.10 ABTS of the present invention + Free radical scavenging rate graph.

[0042] Fig.11 This is the appearance of the dispersion of Example 1 and Example 2 of the present invention. DETAILED DESCRIPTION

[0043] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the specific implementation methods of the present invention are described in detail below in conjunction with the embodiments of the specification.

[0044] In the following description, many specific details are set forth to facilitate a full understanding of the present invention, but the present invention may also be implemented in other ways different from those described herein, and those skilled in the art may make similar generalizations without violating the connotation of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0045] Secondly, the term "one embodiment" or "embodiment" as used herein refers to a specific feature, structure or characteristic that may be included in at least one implementation of the present invention. The term "in one embodiment" that appears in different places in this specification does not refer to the same embodiment, nor is it a separate or selective embodiment that is mutually exclusive with other embodiments. The raw materials in the embodiments of the present invention are all common commercially available products.

[0046] Example 1

[0047] The present embodiment prepares γ-polyglutamic acid / zein composite nanoparticles, which specifically includes the following steps:

[0048] (1) Weighing 0.1 g of zein, adding it to 20 mL of ethanol solution (80 vol%), stirring at room temperature to dissolve it, and then filtering to remove undissolved impurities to obtain a zein solution;

[0049] (2) Weigh 2.5 mg of γ-polyglutamic acid and add it to 60 mL of deionized water, stir at room temperature to fully dissolve it to obtain a γ-polyglutamic acid solution, and then adjust the pH of the solution to 4 with hydrochloric acid;

[0050] (3) under stirring, quickly pouring 20 mL of the zein solution obtained in step (1) into 60 mL of the γ-polyglutamic acid solution obtained in step (2), mixing evenly, and then rotary evaporating in a 40° C. water bath to remove ethanol in the solution;

[0051] (4) Centrifuging the solution obtained in step (3) at 1000 r / min for 10 min to remove possible aggregates, thereby obtaining a γ-polyglutamic acid / zein composite nanoparticle dispersion, wherein the mass ratio of zein to γ-polyglutamic acid is 40:1.

[0052] (5) The γ-polyglutamic acid / zein composite nanoparticle dispersion obtained in step (4) is unstable and flocculation and precipitation occur. Fig.11 shown.

[0053] Example 2

[0054] The method for preparing γ-polyglutamic acid / zein composite nanoparticles in this embodiment uses the same raw materials and process steps as those in Example 1, except that:

[0055] The amount of γ-polyglutamic acid added in the preparation of γ-polyglutamic acid solution is 5 mg, and the mass ratio of zein to γ-polyglutamic acid is 20:1; the obtained γ-polyglutamic acid / zein composite nanoparticle dispersion is unstable and flocculation and precipitation occur, such as Fig.11 shown.

[0056] Example 3

[0057] The method for preparing γ-polyglutamic acid / zein composite nanoparticles in this embodiment uses the same raw materials and process steps as those in Example 1, except that:

[0058] The amount of γ-polyglutamic acid added in the preparation of γ-polyglutamic acid solution is 12.5 mg, and the mass ratio of zein to γ-polyglutamic acid is 8:1; the particle size of the obtained γ-polyglutamic acid / zein composite nanoparticles is about 171.59 nm.

[0059] Example 4

[0060] The method for preparing γ-polyglutamic acid / zein composite nanoparticles in this embodiment uses the same raw materials and process steps as those in Example 1, except that:

[0061] In the preparation of the γ-polyglutamic acid solution, the amount of γ-polyglutamic acid added is 25 mg, and the mass ratio of zein to γ-polyglutamic acid finally obtained is 4:1.

[0062] Example 5

[0063] The method for preparing γ-polyglutamic acid / zein composite nanoparticles in this embodiment uses the same raw materials and process steps as those in Example 1, except that:

[0064] The amount of γ-polyglutamic acid added in the preparation of the γ-polyglutamic acid solution is 50 mg, and the mass ratio of zein to γ-polyglutamic acid is 2: 1. The particle size of the obtained γ-polyglutamic acid / zein composite nanoparticles is 168.68 nm.

[0065] Example 6

[0066] The method for preparing γ-polyglutamic acid / zein composite nanoparticles in this embodiment uses the same raw materials and process steps as those in Example 1, except that:

[0067] In the preparation of the γ-polyglutamic acid solution, the amount of γ-polyglutamic acid added was 100 mg, and the mass ratio of zein to γ-polyglutamic acid was 1:1; the particle size of the obtained γ-polyglutamic acid / zein composite nanoparticles was 201.95 nm.

[0068] Comparative Example 1

[0069] The raw materials and process steps used in the zein nanoparticles prepared in this comparative example are basically the same as those in Example 1, except that:

[0070] The amount of γ-polyglutamic acid added in the preparation of the γ-polyglutamic acid solution is 0; the particle size of the obtained zein nanoparticles is 88.22 nm.

[0071] Comparative Example 2

[0072] This comparative example prepares curcumin-loaded γ-polyglutamic acid / zein composite nanoparticles, which specifically includes the following steps:

[0073] Weigh 0.1 g of zein and 5 mg of curcumin into 20 mL of ethanol solution (80 vol%), stir at room temperature to dissolve them, and then filter to remove undissolved impurities to obtain a zein-curcumin solution;

[0074] Weigh 25 mg of γ-polyglutamic acid and add it to 60 mL of deionized water, stir at room temperature to fully dissolve it to obtain a γ-polyglutamic acid solution, and then adjust the pH of the solution to 4 with hydrochloric acid;

[0075] The curcumin-loaded γ-polyglutamic acid / zein composite nanoparticle dispersion can be prepared by the same method as (3) and (4) in Example 1.

[0076] Comparative Example 3

[0077] This comparative example only uses free curcumin to carry out various index identification.

[0078] The various indicators in the various substances in Examples 1-6 and Comparative Examples 1-2 were identified, comprising the following steps:

[0079] 1. Determination of the particle size of composite nanoparticles

[0080] Dilute 1 mg / mL nanoparticle dispersion 20 times with deionized water of the same pH, take 3 mL of the dispersion in a cuvette, and use NanoBrook Omni light scattering particle size analyzer to measure the particle size of the sample;

[0081] The parameters were set as follows: test temperature 25°C, equilibrium time 30s, detection angle 90°, test time 120s, and three measurements;

[0082] 2. Stability evaluation of composite nanoparticles

[0083] (1) pH stability

[0084] The pH of the composite nanoparticle dispersion was adjusted to 3, 4, 5, 6, 7, 8, or 9 with 0.1 M HCl or 0.1 M NaOH. After standing at room temperature for 24 h, the particle size was sampled and tested to determine the pH stability of the composite nanoparticle dispersion. The results of Example 4 are shown in Figure 1 .

[0085] (2) Ionic stability

[0086] Two groups of composite nanoparticle dispersions with pH values ​​of 4 and 7 were selected, and then sodium chloride aqueous solutions with the same pH and volume but different concentrations were added to make the final concentration of the composite nanoparticle dispersion 1 mg / mL, while the concentrations of sodium chloride were 0 mM, 100 mM, 200 mM, 500 mM, and 1000 mM, respectively. After standing at room temperature for 24 hours, samples were taken to test the particle size to determine the ionic stability of the composite nanoparticle dispersion. The results are shown in Figure 2 and Figure 3 (Example 4).

[0087] (3) Thermal stability

[0088] Two groups of composite nanoparticle dispersions with pH values ​​of 4 and 7 were selected, and then placed in an 80°C water bath for heating for 0 min, 10 min, 20 min, 30 min, 40 min, 50 min, 60 min, and 120 min, respectively. After standing at room temperature for 24 h, samples were taken to test the particle size to determine the thermal stability of the composite nanoparticle dispersion. The results are shown in Figure 4 (Example 4).

[0089] (4) Storage stability

[0090] Three groups of composite nanoparticle dispersions with pH values ​​of 4, 7, and 9 were selected and stored at 4°C. Samples were taken at regular intervals to test the particle size to determine the storage stability of the nanoparticle dispersions. The results are shown in Figure 5 (Example 4).

[0091] 3. Determination of curcumin encapsulation efficiency and drug loading

[0092] Ethanol solution (80 vol%) was used to prepare curcumin solutions of different concentrations, and the absorbance was measured at 430 nm to obtain the standard curve of curcumin:

[0093] y=0.0266x-0.0004,R 2 =1;

[0094] Take 200 μL of the composite nanoparticle dispersion loaded with curcumin, add 800 μL of anhydrous ethanol, and sonicate for 5 minutes to fully dissolve the zein and release curcumin. After appropriate dilution with ethanol solution (80 vol%), measure the absorbance at 430 nm with a microplate reader, and calculate the concentration of curcumin through the standard curve (to avoid photodegradation of curcumin, the whole process was operated under dark conditions).

[0095] Calculated by the following formula, the encapsulation efficiency of curcumin was 95.93±2.07%, and the drug loading rate was 3.84±0.08%.

[0096] Encapsulation efficiency (%) = mass of encapsulated curcumin / mass of added curcumin × 100%

[0097] Drug loading rate (%) = mass of embedded curcumin / sum of mass of Zein and γ-polyglutamic acid in composite nanoparticles × 100%.

[0098] 4. Scanning Electron Microscope Analysis

[0099] The freeze-dried composite nanoparticle powder samples with and without curcumin were fixed on the conductive glue and then sprayed with gold. The sample morphology was observed and photographed by a HITACHI S-4800 scanning electron microscope at a voltage of 3KV. The results are shown in Figure 6 (Example 4 and Comparative Example 2).

[0100] 5. Cytocompatibility Assessment

[0101] The cell compatibility of the composite nanoparticle carrier was evaluated by measuring the cytotoxicity of the composite nanoparticles to HaCaT (keratinocytes). HaCaT cells were seeded into 96-well plates at a density of 10,000 cells per well and incubated in a 37°C constant temperature incubator for 24 hours. The old culture medium was removed and 100 μL of composite nanoparticle dispersions of different concentrations were added to incubate HaCaT cells for 24 hours or 48 hours. The composite nanoparticle dispersion was then poured out, the cells were washed with PBS, 100 μL of CCK-8 working solution was added to each well, and the plates were incubated in a 37°C constant temperature incubator for 30 minutes. The absorbance of each well at 450 nm was measured using an ELISA reader.

[0102] The CCK-8 working solution consisted of 10% (v / v) CCK-8 reagent and 90% (v / v) complete cell culture medium.

[0103] Cell survival rate / % = (A S -A B ) / (A C -A B )×100%

[0104] Where: A C A is the negative control group (100 μL of fresh culture medium was used instead of the composite nanoparticle dispersion); S is the absorbance value of the composite nanoparticle dispersion after treatment; A B is the absorbance value of 100 μL CCK-8 working solution.

[0105] The results of cell compatibility analysis are shown in Figure 7 (Example 4).

[0106] 6. Study on thermal stability of curcumin in composite nanoparticles

[0107] The composite nanoparticle dispersion loaded with curcumin was placed in a sample bottle and heated in a water bath at 80°C. Samples were taken at 0h, 2h, 4h, 6h, 8h, and 10h to detect the content of curcumin in the composite nanoparticle dispersion. A curcumin solution with the same concentration as in the experimental system was prepared, and the solvent was ethanol solution (80 vol%). This was used as a control experimental group, and its thermal stability results are shown in Figure 8 (Comparative Example 2)

[0108] 7. Evaluation of Antioxidant Activity

[0109] (1) DPPH free radical scavenging activity

[0110] Dissolve DPPH in anhydrous ethanol to prepare a 0.1 mM DPPH working solution.

[0111] Control sample (A C ): 400 μL deionized water, 1.6 mL DPPH working solution. Test sample (A S ): 400 μL of the composite nanoparticle dispersion loaded with curcumin, 1.6 mL of DPPH·working solution. Blank sample (A B ): 400 μL of the composite nanoparticle dispersion loaded with curcumin, 1.6 mL of anhydrous ethanol. The above solutions were mixed, vortexed evenly, sonicated for 5 minutes, incubated for 25 minutes in the dark, and 100 μL was pipetted with a pipette, and the absorbance at 517 nm was measured using an ELISA reader.

[0112] DPPH·free radical scavenging rate (%) = (A C -(A S -A B )) / A C ×100%

[0113] A C : absorbance of DPPH·working solution at 517nm; A S : Absorbance at 517 nm after the reaction between DPPH·working solution and the composite nanoparticle dispersion loaded with curcumin; A B : Absorbance of the composite nanoparticle dispersion loaded with curcumin at 517 nm.

[0114] (2)ABTS + Free radical scavenging activity

[0115] Mix 7.0 mM ABTS aqueous solution and 4.9 mM K2S2O8 aqueous solution in equal volumes and leave to stand at room temperature in the dark for 24 h to obtain ABTS + Solution. Dilute 40 times with 80% (v / v) ethanol solution to obtain ABTS + Working solution. Control sample (A C): 400 μL deionized water, 1.6 mL ABTS + Working fluid. Test sample (A S ): 400 μL of composite nanoparticle dispersion loaded with curcumin, 1.6 mL of ABTS + Working solution. Blank sample (A B ): 400 μL of the composite nanoparticle dispersion loaded with curcumin, 1.6 mL of 80% ethanol. After mixing the above solutions, vortex evenly, sonicate for 5 minutes, incubate for 25 minutes in the dark, pipette 100 μL, and measure the absorbance at 734 nm using an ELISA reader. ABTS + The calculation formula for free radical scavenging is as follows:

[0116] ABTS + ·Free radical scavenging rate (%) = (A C -(A S -A B )) / A C ×100%

[0117] A C :ABTS + Absorbance of working solution at 734nm; A S :ABTS + Absorbance at 734 nm after the working solution reacts with the composite nanoparticle dispersion loaded with curcumin; A B : Absorbance of the composite nanoparticle dispersion loaded with curcumin at 734 nm.

[0118] The results of antioxidant activity evaluation are shown in Fig. 9 and Fig.10 (Comparative Example 2).

[0119] Preparation of free curcumin: Prepare a curcumin solution with the same concentration as in the experimental system, and use ethanol solution (80 vol%) as the solvent, as a control experimental group, and its thermal stability results are shown in Figure 8 .

[0120] The present invention proposes for the first time a method for preparing γ-polyglutamic acid / zein composite nanoparticles, which is simple to operate, low in cost and easy to mass produce; the γ-polyglutamic acid / zein composite nanoparticles prepared by the present invention have excellent stability, and compared with single alcohol-soluble zein nanoparticles or existing composite nanoparticles, the γ-polyglutamic acid / zein composite nanoparticles can remain stable in a wider range of pH, ionic strength and temperature.

[0121] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention can be modified or replaced by equivalents without departing from the spirit and scope of the technical solutions of the present invention, which should all be included in the scope of the present invention.

Claims

1. A method for preparing γ-polyglutamic acid / zein composite nanoparticles, characterized in that: include, dissolving zein in an ethanol solution to obtain a zein solution; dissolving γ-polyglutamic acid in deionized water to obtain a γ-polyglutamic acid solution; The zein solution is added to the γ-polyglutamic acid solution, ethanol in the solution is evaporated away, and insoluble matter is removed by centrifugation to obtain a γ-polyglutamic acid / zein composite nanoparticle dispersion; The γ-polyglutamic acid / zein composite nanoparticle powder was obtained by freeze drying.

2. The preparation method according to claim 1, characterized in that: The concentration of the γ-polyglutamic acid solution is 0.01-2 wt %.

3. The preparation method according to claim 1 or 2, characterized in that: The concentration of the zein solution is 0.1-10 wt %.

4. The preparation method according to claim 3, characterized in that: The molecular weight of the gamma-polyglutamic acid is 300,000 to 2,000,000 g / mol.

5. The preparation method according to claim 1, characterized in that: The zein solution is added into the gamma-polyglutamic acid solution, wherein the mass ratio of the gamma-polyglutamic acid to the zein is 1:40 to 2:

1.

6. The preparation method according to claim 1 or 5, characterized in that: The pH of the γ-polyglutamic acid solution is 3-6.

7. A method for preparing γ-polyglutamic acid / zein composite nanoparticles loaded with biologically active ingredients, characterized in that: include, dissolving zein in an ethanol solution to obtain a zein solution, and dissolving a bioactive component in the zein solution to obtain a zein solution containing the bioactive component; dissolving γ-polyglutamic acid in deionized water to obtain a γ-polyglutamic acid solution; Adding a zein solution containing bioactive ingredients into a γ-polyglutamic acid solution, evaporating to remove ethanol in the solution, and centrifuging to remove insoluble matter to obtain a γ-polyglutamic acid / zein composite nanoparticle dispersion loaded with bioactive ingredients; The γ-polyglutamic acid / zein composite nanoparticle powder loaded with bioactive components is obtained by freeze drying.

8. The preparation method according to claim 7, characterized in that: The mass ratio of the biologically active ingredient to zein is 1:50 to 1:

2.

9. The preparation method according to claim 7 or 8, characterized in that: The bioactive ingredients include curcumin, catechin, EGCG, quercetin and baicalin.

10. Use of the γ-polyglutamic acid / zein composite nanoparticles loaded with bioactive ingredients prepared by the preparation method according to any one of claims 7 to 9 in the preparation of foods, cosmetics and medicines.

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