Externally applied ointment for treating venomous snake bites and preparation method thereof

Abstract

The invention provides an externally applied ointment for treating venomous snake bites and a preparation method thereof, and belongs to the field of pharmaceutical compositions. The external application ointment is prepared from the following raw material medicines in parts by weight: 90 to 120 parts of herba scutellariae barbatae, 90 to 120 parts of herba hedyotidis diffusae, 90 to 120 parts of herba violae, 90 to 120 parts of herba taraxaci, 90 to 120 parts of raw liquorice root, 90 to 120 parts of herba plantaginis, 90 to 120 parts of caulis akebiae and 90 to 120 parts of radix et rhizoma rhei. The medicinal materials of the external application ointment supplement each other, and the external application ointment is safe and effective, and has a good application prospect in preparation of drugs for treating venomous snake bites.

Description

Technical Field

[0001] The invention belongs to the field of pharmaceutical compositions, and in particular relates to an external ointment for treating venomous snake bites and a preparation method thereof. Background Art

[0002] Venomous snake bites are a serious acute medical emergency. There are more than 100 components in snake venom, mainly divided into neurotoxins, hematotoxins and cytotoxins. Clinically, the wound is generally swollen and stinging, spreading outward, often accompanied by subcutaneous hemorrhagic ecchymosis and continuous bleeding. Systemic poisoning reactions occur 1 to 6 hours after the bite, including blurred vision, diplopia, ptosis, impaired movement of the injured limb, dyspnea, oliguria or anuria, and soy sauce-like urine. In severe cases, it may manifest as abnormal neurological function, respiratory distress, hemodynamic instability and shock. Therefore, timely and effective treatment is crucial to reduce the disability and mortality rate of venomous snake bites.

[0003] At present, the treatment of snake bites mainly includes local treatment, anti-venom serum treatment, traditional Chinese medicine treatment and auxiliary treatment. Local treatment usually includes wound cleaning, ligation, raising the affected limb and other measures to slow down the spread of toxins. Anti-venom serum is the main method for treating snake venom at present, but it can only neutralize free toxins in the body, while toxins bound to tissues and organs will cause organ damage to patients, greatly increasing the limb disability rate of patients.

[0004] Traditional Chinese medicine has a long history and rich experience in the treatment of venomous snake bites. Traditional Chinese medicine believes that snakes are fireflies, which are the most poisonous. The basic pathogenesis of venomous snake bites is "the snake venom is not discharged, and the toxic evil is accumulated inside". The common clinical symptoms of snake bites are wind and fire toxicity syndrome. In order to reduce the limb disability rate of patients, it is necessary to carry out heat-clearing and detoxification, blood circulation and blood stasis, swelling and pain relief treatment on the basis of Western medicine treatment. Therefore, it is of great clinical significance to develop a safe and effective snake bite ointment with Chinese medicine characteristics. Summary of the invention

[0005] The invention aims to provide an external ointment for treating snake bites and a preparation method thereof.

[0006] The invention provides a pharmaceutical composition, which is prepared from raw materials in the following mass ratio: Scutellaria barbata: Hedyotis diffusa: Viola yedoensis: Taraxacum officinale: Licorice root: Plantago: Akebia: Rhubarb = 90-120: 90-120: 90-120: 90-120: 90-120: 90-120: 90-120: 90-120: 90-120: 90-120.

[0007] Furthermore, the mass ratio of the Scutellaria barbata, Oldenlandia diffusa, Viola yedoensis, Taraxacum officinale, Licorice root, Plantago, Akebia and Rhubarb is 1-2:1-2:1-2:1:1:1:1:1:1.

[0008] Furthermore, the mass ratio of the Scutellaria barbata, Oldenlandia diffusa, Viola yedoensis, Taraxacum officinale, Licorice root, Plantago, Akebia and Rhubarb is 1:1:1:1:1:1:1:1:1:1.

[0009] The present invention also provides an external application ointment, which is a pharmaceutical preparation prepared from the above-mentioned pharmaceutical composition and an ointment matrix; wherein the mass of the ointment matrix accounts for 30-70% of the total mass of the pharmaceutical composition.

[0010] Furthermore, the ointment base is vaseline.

[0011] The present invention also provides a method for preparing the above-mentioned external application ointment, which comprises the following steps: weighing the raw material medicine according to the weight ratio, drying, crushing and screening; mixing the raw material medicine with the molten ointment base to form a paste ointment; cooling to obtain the external application ointment.

[0012] Furthermore, the drying conditions are: drying at 50-70° C. for 2-4 days.

[0013] Furthermore, the drying condition is: drying at 60° C. for 3 days.

[0014] Furthermore, the conditions for the pulverizing and screening are: coarse pulverizing first, then fine pulverizing, and passing through a 80-120 mesh sieve.

[0015] Furthermore, the conditions for the pulverizing and screening are: the pulverizing time for coarse pulverizing is 5 minutes, the pulverizing time for fine pulverizing is 10 minutes, and the pulverizing is passed through a 100-mesh sieve.

[0016] The present invention also provides use of the above-mentioned pharmaceutical composition and the above-mentioned external application ointment in preparing a medicine for treating venomous snake bites.

[0017] In the formula of the specific embodiment of the present invention, "part" means part by weight, and 1 part is 1 g.

[0018] The formula of the invention comprises: 90-120g of Scutellaria barbata, 90-120g of Hedyotis diffusa, 90-120g of Viola yedoensis, 90-120g of Taraxacum mongolicum, 90-120g of raw licorice, 90-120g of Plantain, 90-120g of Akebia and 90-120g of Rhubarb.

[0019] According to the principles of TCM compatibility and classic herbal medicine works, the effects of each Chinese medicine used in the present invention are as follows:

[0020] Scutellaria barbata: pungent, bitter, cold in nature, enters the lung, liver, and kidney meridians, clears away heat, detoxifies, and promotes blood circulation and removes blood stasis;

[0021] Hedyotis diffusa: sweet, light, cold in nature, enters the heart, liver, and spleen meridians, clears away heat and detoxifies, and promotes the flow of dampness and relieves stranguria;

[0022] Dandelion: bitter and sweet, cold in nature, enters the liver and stomach meridians, clears away heat and detoxifies, and promotes the flow of dampness and relieves stranguria;

[0023] Raw licorice: sweet, neutral in nature, enters the heart, lung, spleen, and stomach meridians, clears away heat and detoxifies, relieves urgency and relieves pain;

[0024] Viola yedoensis: pungent, bitter, cold in nature, enters the heart and liver meridians, clears away heat, detoxifies, cools blood and reduces swelling;

[0025] Plantain: sweet in taste, cold in nature, enters the liver, kidney, lung, and small intestine meridians, promotes diuresis, relieves stranguria, clears the liver and improves eyesight;

[0026] Akebia: bitter, cold in nature, enters the heart, small intestine, and bladder meridians, promotes diuresis, relieves stranguria, and clears heart fire;

[0027] Rhubarb: bitter in taste, cold in nature, enters the spleen, stomach, large intestine, liver, and pericardium meridians, clears away heat and purges fire, cools blood and detoxifies, removes blood stasis and dredges the menstruation.

[0028] The present invention has achieved the following beneficial effects:

[0029] The present invention provides an external ointment for treating venomous snake bites and a preparation method thereof. The external ointment is prepared from the following raw materials in weight proportion: 90-120 parts of Scutellaria barbata, 90-120 parts of Hedyotis diffusa, 90-120 parts of Viola yedoensis, 90-120 parts of Taraxacum officinale, 90-120 parts of raw licorice, 90-120 parts of Plantago, 90-120 parts of Akebia, and 90-120 parts of Rhubarb. The external ointment of the present invention has complementary medicinal flavors, is safe and effective, and has good application prospects in the preparation of drugs for treating venomous snake bites.

[0030] Obviously, according to the above contents of the present invention, in accordance with common technical knowledge and customary means in the art, without departing from the above basic technical ideas of the present invention, other various forms of modification, replacement or change may be made.

[0031] The above contents of the present invention are further described in detail below through specific implementation methods in the form of examples. However, this should not be understood as the scope of the above subject matter of the present invention being limited to the following examples. All technologies realized based on the above contents of the present invention belong to the scope of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1 The present invention is a schematic diagram of the process of preparing an external application ointment.

[0033] Figure 2 This is the patient's limb before applying topical ointment.

[0034] Figure 3 For applying topical ointment to the patient's limbs.

[0035] Figure 4For the limbs of patients treated with topical ointments for several days.

[0036] Figure 5 Comparison of inflammatory indicators before and after external ointment treatment (compared with before treatment, P<0.05). DETAILED DESCRIPTION

[0037] The raw materials and equipment used in the present invention are all known products, which are obtained by purchasing commercially available products.

[0038] Example 1. Preparation of the external application ointment of the present invention

[0039] according to Figure 1 The process is prepared:

[0040] (1) Weigh 90 g of Scutellaria barbata and weigh other medicinal materials according to the mass ratio (Scutellaria barbata: Hedyotis diffusa: Viola yedoensis: Taraxacum officinale: Licorice root: Plantago: Akebia: Rhubarb = 1:1:1:1:1:1:1:1:1);

[0041] (2) Dry the medicinal materials in an oven at 60°C for 3 days;

[0042] (3) Place each medicinal material in a traditional Chinese medicine grinder for coarse grinding for 5 minutes to obtain coarse powder, then place the coarse powder in a water-cooled low-temperature grinder for fine grinding for 10 minutes, pass through a 100-mesh sieve to obtain fine powder, and can it for later use;

[0043] (4) Put 50% of the total amount of vaseline in a stainless steel pot and heat it slowly at 60-80°C over low heat to melt the vaseline. The temperature should not be too high to prevent the vaseline from burning or decomposing, which will affect the quality and viscosity of the plaster.

[0044] (5) Take 50 g of the fine powder of the medicinal material and add it to the melted vaseline, stirring while adding to avoid powder flying or bubbles forming, until the mixture is evenly mixed and adjusted into a paste-like ointment. After cooling, an external application ointment is obtained.

[0045] When using, take an appropriate amount of fine powder, add vaseline or other bases and mix well. Avoid wounds and blisters when applying, and apply evenly around the wound and 3-4 cm above the upper end of the swollen limb, twice a day, until the redness, swelling and pain of the limb are eliminated.

[0046] The following is a control sample preparation.

[0047] Comparative Example 1, Preparation of External Application Ointment

[0048] The method of reference example 1 is different in that medicinal materials with the same total mass as those in example 1 are weighed according to the mass ratio (Scutellaria barbata: Hedyotis diffusa: Viola yedoensis: Taraxacum officinale: Licorice root: Plantago: Akebia: Rhubarb = 0.5:0.5:0.5:1:1:1:1:1) to obtain an external ointment.

[0049] Comparative Example 2: Preparation of external application ointment

[0050] The method of reference example 1 is different in that medicinal materials with the same total mass as those in example 1 are weighed according to the mass ratio (Scutellaria barbata: Hedyotis diffusa: Viola yedoensis: Taraxacum officinale: Licorice root: Plantago: Akebia: Rhubarb = 2:2:2:1:1:1:1:1) to obtain an external application ointment.

[0051] Comparative Example 3: Preparation of external application ointment

[0052] The method of reference example 1 is different in that medicinal materials with the same total mass as those in example 1 are weighed according to the mass ratio (Scutellaria barbata: Hedyotis diffusa: Viola yedoensis: Taraxacum officinale: Licorice root: Plantago: Akebia: Rhubarb = 4:4:4:1:1:1:1:1) to obtain an external ointment.

[0053] The beneficial effects of the present invention are demonstrated by experimental examples below.

[0054] Experimental Example 1: Effect of the topical ointment of the present invention on treating snake bites

[0055] 1. Experimental methods

[0056] 1.1 General Information

[0057] A total of 53 patients with snake bites were selected, including 18 males and 35 females. The youngest was 10 years old and the oldest was 83 years old, with an average age of 63 years old. The bite sites included fingers in 11 cases, dorsal hands in 6 cases, forearm in 1 case, toes in 2 cases, dorsal feet in 10 cases, ankles in 16 cases, and calves in 7 cases. The average hospital stay was 9 days.

[0058] 1.2 Treatment methods

[0059] (1) Wound treatment: early ligation, wound enlargement and detoxification, and wound flushing with saline, hydrogen peroxide solution, etc. to remove residual venom and other contaminants;

[0060] (2) Basic treatment: select the corresponding antivenom serum according to the type of snake, or conduct preventive anti-infection treatment;

[0061] (3) External application of Chinese medicine: Take the external application ointment prepared in Example 1 and apply it evenly around the wound and 3-4 cm above the swollen limb, twice a day.

[0062] 2. Experimental results

[0063] like Figure 2 , Figure 3 and Figure 4 As shown in the figure, after applying the ointment, the patient's systemic poisoning symptoms disappeared, limb swelling and ecchymosis subsided, and the pain was effectively relieved. After recovery, no local or systemic sequelae occurred. Figure 5 As shown in the data, the inflammatory indicators [white blood cell count (WBC) and neutrophil ratio (NEU%)] after treatment with the topical ointment were significantly lower than those before treatment (P<0.05), indicating that the topical ointment has a significant anti-inflammatory and analgesic effect, and can clear away heat, detoxify, reduce swelling and relieve pain in snake bites.

[0064] Experimental Example 2: Effect of external application of ointment in treating snake bites

[0065] 1. Experimental methods

[0066] Each group included 10 patients with snake bites. The difference in the number of white blood cells (WBC) in each group was measured before and after applying the ointment. WBC and the change difference of neutrophil percentage (NEU%)△ NEU％ , as shown in Table 1.

[0067] Measurement data were expressed as mean ± standard deviation If the data are normally distributed, the t test is used. If the data are not normally distributed, the rank sum test is used. The count data are analyzed using the χ 2 P<0.05 was considered statistically significant.

[0068] 2. Experimental results

[0069] Table 1 WBC, NEU% and △ of each group before and after treatment WBC ,△ NEU％ Level comparison

[0070]

[0071] Note: Compared with the group before treatment, *P<0.05.

[0072] Compared with the external application ointment of control example 1 and control example 3, the effect of the external application ointment of Example 1 and control example 2 on the treatment of venomous snake bites was significantly improved, and the effect of reducing WBC and reducing NEU% was significantly improved. Compared with before treatment, the levels of WBC and NEU% in Example 1 and control example 2 were reduced after external application of ointment (P < 0.05); in terms of reducing WBC, Example 1△ WBC Compared with control example 2 WBC In terms of reducing NEU%, Example 1△ NEU％ Greater than control example 2△ NEU％In terms of formula composition, Example 1 reduces the amount of the main medicine (Scutellaria barbata, Hedyotis diffusa and Viola yedoensis) used compared to Control Example 2. In summary, the external application ointment prepared using the formula ratio of Example 1 has a better effect of reducing inflammatory indicators (white blood cell count and neutrophil count).

[0073] In summary, the present invention provides an external ointment for treating venomous snake bites and a preparation method thereof. The external ointment of the present invention is prepared from the following raw materials in weight ratio: 90-120 parts of Scutellaria barbata, 90-120 parts of Hedyotis diffusa, 90-120 parts of Viola yedoensis, 90-120 parts of Taraxacum officinale, 90-120 parts of raw licorice, 90-120 parts of Plantago, 90-120 parts of Akebia, and 90-120 parts of Rhubarb. The external ointment of the present invention has complementary medicinal flavors, is safe and effective, and has good application prospects in the preparation of drugs for treating venomous snake bites.

Claims

1. A pharmaceutical composition, characterized in that The drug is prepared from raw materials in the following mass ratio: Scutellaria barbata: Hedyotis diffusa: Viola yedoensis: Taraxacum officinale: Licorice root: Plantago: Akebia: Rhubarb = 90-120: 90-120: 90-120: 90-120: 90-120: 90-120: 90-120: 90-120: 90-120.

2. The pharmaceutical composition according to claim 1, characterized in that The mass ratio of the Scutellaria barbata, Hedyotis diffusa, Viola yedoensis, Taraxacum officinale, Licorice root, Plantago, Akebia and Rhubarb is 1-2:1-2:1-2:1:1:1:1:1:

1.

3. The pharmaceutical composition according to claim 2, characterized in that The mass ratio of the Scutellaria barbata, Hedyotis diffusa, Viola yedoensis, Taraxacum officinale, Licorice root, Plantago, Akebia and Rhubarb is 1:1:1:1:1:1:1:1:

1.

4. An external application ointment, characterized in that: The pharmaceutical preparation is prepared from the pharmaceutical composition according to any one of claims 1 to 3 and an ointment matrix; wherein the mass of the ointment matrix accounts for 30 to 70% of the total amount of the pharmaceutical composition.

5. The external application ointment according to claim 4, characterized in that The ointment base is vaseline.

6. A method for preparing the external application ointment according to any one of claims 4 to 5, characterized in that: The method comprises the following steps: weighing the raw material medicine according to the weight ratio, drying, crushing and screening; mixing the raw material medicine with the melted ointment base to form a paste ointment; cooling to obtain the external application ointment.

7. The method according to claim 6, characterized in that The drying conditions are: drying at 50-70° C. for 2-4 days.

8. The method according to claim 6, characterized in that The conditions for the pulverization and screening are: coarse pulverization first, then fine pulverization, and passing through a 80-120 mesh sieve.

9. Use of the pharmaceutical composition according to any one of claims 1 to 3 and the external application ointment according to any one of claims 4 to 5 in the preparation of a medicament for treating venomous snake bites.

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