Veterinary Siji decoction and preparation method thereof

Abstract

The invention belongs to the technical field of traditional Chinese medicine compositions, and particularly relates to a veterinary Siji decoction and a preparation method thereof, the preparation method comprises the following steps: (1) mixing dried ginger and honey-fried licorice root, boiling with water to obtain a water extract, concentrating, carrying out alcohol extraction, and filtering to obtain a dried ginger and honey-fried licorice root extract mixture; (2) crushing and sieving radix aconiti carmichaeli to obtain radix aconiti carmichaeli particles, soaking the radix aconiti carmichaeli particles in deionized water, adding cane sugar, corn steep liquor, vitamin H, monopotassium phosphate, ammonium citrate and magnesium carbonate, and sterilizing to obtain a culture medium to be inoculated; (3) adding a mixed strain into the culture medium to be inoculated, and standing for fermentation to obtain a fermentation mixture; and (4) mixing the dried ginger and honey-fried licorice root extract mixture with the fermentation mixture to obtain the traditional Chinese medicine composition. The veterinary Siqi decoction provided by the invention is high in active ingredient content, not easy to precipitate, high in storage stability and almost non-toxic, and can effectively improve the health degree of young children, reduce the feed conversion ratio and morbidity and death rate and improve the economic benefits of livestock farms when being used for prevention and health care of poultry and livestock.

Description

Technical Field

[0001] The invention belongs to the technical field of traditional Chinese medicine compositions, and particularly relates to a veterinary Sini decoction and a preparation method thereof. Background Art

[0002] In the poultry and livestock farming industry, as the farming model develops towards modernization and high-density models, the health of chicks is easily affected by environmental factors. In addition, the physiological characteristics of piglets and chicks, such as their own incomplete development, low digestive skills and insufficient immunity, as well as the long-term and large-scale abuse of antibiotics in the farming system, make chicks very susceptible to disease and high mortality. The health of chicks in the first few days after birth is directly related to their later growth and slaughter, affecting the economic benefits of the farm. Therefore, countries around the world have begun to adopt antibiotic-free programs for farming to give full play to the role of medicine and food. However, directly stopping the use of antibiotics will obviously cause a greater decline in breeding benefits.

[0003] Especially for the situation that the growth rate of chicks is too fast and the cardiopulmonary function is not matched, the existing technology often uses Sini Decoction to improve the health of chicks. Sini Decoction is included in the second part of the 2020 edition of the "Chinese Veterinary Pharmacopoeia", and the formula is composed of light aconite, dried ginger and roasted licorice. The invention patent with application number 201710142172.6 discloses a method for preventing and controlling common diseases of broilers using Chinese veterinary oral liquid, which uses Sini Decoction for broilers from 1 to 7 days old; Huangzhi oral liquid for broilers from 15 to 21 days old; Qingwen Jiedu oral liquid for broilers from 35 to 42 days old. This invention uses Chinese veterinary oral liquid to administer drugs to broilers in stages, which can not only prevent and control common diseases in broiler breeding and improve broiler disease resistance, but also increase broiler output rate, feed-to-meat ratio, and daily weight gain, and reduce veterinary drug residues in broiler products, playing a positive role in promoting the healthy development of the broiler industry; the invention patent with application number 202310627616.0 discloses a preparation method and a Sini Decoction preparation, which, by subjecting the water extraction mixture to a primary heat concentration treatment and the alcohol precipitation filtrate to a secondary heat concentration treatment, and adding a mixing and co-heating step after adding the flavoring agent solution, can cause the medicinal solution to reach dynamic equilibrium in thermodynamics and kinetics, thereby significantly improving the stability and clarity of Sini Decoction.

[0004] However, the current Sini Decoction Chinese medicine composition often directly extracts the active ingredients of each Chinese medicine in the formula, which will result in insufficient utilization of the active ingredients of Sini Decoction and easy precipitation of the finished product. In addition, when the existing Sini Decoction Chinese medicine composition is used for the prevention and health care of poultry and livestock, the toxic Chinese medicine ingredients that may accumulate due to dosage adjustment will also affect the health of young poultry and livestock. Summary of the invention

[0005] In view of the defects of the prior art, the present invention provides a veterinary Sini Decoction and a preparation method thereof. The veterinary Sini Decoction disclosed by the present invention has a high content of active ingredients, is not easy to precipitate, has high storage stability, and is almost non-toxic. When used for the prevention and health care of poultry and livestock, it can effectively improve the health of chicks, reduce the feed-to-meat ratio and the mortality rate, and improve the economic benefits of the farm.

[0006] In order to achieve the above object, the technical solution adopted by the present invention is as follows:

[0007] In one aspect, the present invention provides a method for preparing Sini Decoction for animals, the method comprising the following steps:

[0008] (1) boiling a mixture of dried ginger and roasted licorice to obtain a water extract, and concentrating, alcohol extracting and filtering to obtain a dried ginger and roasted licorice extract mixture;

[0009] (2) crushing and sieving the aconite root to obtain aconite root granules, taking the aconite root granules and soaking them in deionized water, adding sucrose, corn syrup, vitamin H, potassium dihydrogen phosphate, ammonium citrate and magnesium carbonate, adjusting the pH, and sterilizing to obtain a culture medium to be inoculated;

[0010] (3) adding the mixed strains to the culture medium to be inoculated in step (2), allowing the mixture to ferment to obtain a fermentation mixture;

[0011] (4) The dried ginger and roasted licorice root extract mixture in step (1) and the fermented mixture in step (2) are mixed to obtain Sini Decoction for animals.

[0012] Preferably, the operation of boiling the mixture to obtain the water extract is as follows: (a) mixing dried ginger and roasted licorice, adding deionized water and boiling for 1 to 3 hours, filtering to obtain a boiled liquid and a filter residue; (b) adding deionized water to the filter residue and boiling for 1 to 2 hours, filtering to obtain a filter residue boiled liquid, and mixing the boiled liquid and the filter residue boiled liquid to obtain a water extract.

[0013] Preferably, the amount of deionized water added in operation (a) is 5 to 10 times the total mass of dried ginger and roasted licorice.

[0014] Preferably, the amount of deionized water added in the operation (b) is 3 to 5 times the mass of the filter residue.

[0015] In some embodiments of the present invention, the concentration operation is: at a vacuum degree of -0.01 to 0 MPa and a temperature of 60 to 80° C., concentrating for 2 to 10 hours to obtain a concentrate.

[0016] In some embodiments of the present invention, the alcohol extraction is performed by adding 70-90% v / v alcohol solution, and extracting at a temperature of 40-50° C. for 2-4 days.

[0017] Preferably, the 70-90% v / v alcohol solution is a 70-90% v / v ethanol solution.

[0018] Preferably, the amount of the 70-90% v / v alcohol solution added is 3-6 times the volume of the concentrate.

[0019] Preferably, the filtering operation is: first coarse filtration, then filtering through a 0.3-0.5 μm filter membrane and collecting the filtrate, and repeating the above operation at least once.

[0020] In some embodiments of the present invention, the particle size of the aconite granules in step (2) is 10 to 60 meshes.

[0021] Preferably, the particle size of the aconite granules in step (2) is 20 to 60 meshes.

[0022] Further preferably, the particle size of the aconite granules in step (2) is 40 mesh.

[0023] In some embodiments of the present invention, the amount of deionized water added in step (2) is 40-45% of the mass of the aconite granules.

[0024] Preferably, the amount of deionized water added in step (2) is 43% of the mass of the aconite granules.

[0025] In some embodiments of the present invention, the added amounts of sucrose, corn syrup, vitamin H, potassium dihydrogen phosphate, ammonium citrate and magnesium carbonate in step (2) are 0.5-1.5%, 1-2%, 0.01-0.05%, 0.2-0.3%, 0.01-0.15% and 0.1-0.2% of the total mass of the aconite granules and deionized water, respectively.

[0026] Further preferably, in the step (2), the added amounts of sucrose, corn syrup, vitamin H, potassium dihydrogen phosphate, ammonium citrate and magnesium carbonate are 1%, 1.5%, 0.03%, 0.3%, 0.08% and 0.15% of the total mass of the aconite granules and deionized water, respectively.

[0027] Preferably, the sterilization conditions in step (2) are: temperature 110-125° C., and sterilization time 30-60 min.

[0028] In some embodiments of the present invention, the fermentation operation is: fermentation at 20-30° C. for 5-10 days.

[0029] In some embodiments of the present invention, the mixed bacterial species comprises Schizophyllum and Acetobacter pasteurianum.

[0030] In some embodiments of the present invention, the amount of Schizophyllum added is 0.1 to 0.4 cm 2Schizophyllum / g Aconite granules, the amount of Acetobacter pasteurianum added is 0.1~0.2cm 2 Acetobacter pasteurianus / g of Aconitum granules.

[0031] Preferably, the amount of Schizophyllum added is 0.3 cm 2 Schizophyllum / g Aconite granules, the amount of Acetobacter pasteurianum added is 0.2cm 2 Acetobacter pasteurianus / g of Aconitum granules.

[0032] On the other hand, the present invention also provides a veterinary Sini Decoction obtained according to the above preparation method, wherein the veterinary Sini Decoction comprises the following raw materials in parts by weight: 100 to 200 parts of dried ginger, 150 to 300 parts of roasted licorice and 150 to 300 parts of aconite.

[0033] Preferably, the veterinary Sini Decoction comprises the following raw materials in parts by weight: 150 parts of dried ginger, 225 parts of roasted licorice and 225 parts of aconite.

[0034] The present invention extracts active ingredients from dried ginger and licorice by optimizing conditions, improves the utilization rate of dried ginger and licorice raw materials by optimizing concentration and alcohol extraction steps, has high content of effective active ingredients, and has better stability. By utilizing the characteristics of dried ginger extract that it is highly hot and non-toxic and stays but does not move, it exerts the effects of warming yang, keeping the middle and returning yang to the meridians, and utilizes the synergistic effect of the compatibility of dried ginger and aconite to exert a better warming yang and meridians, significantly increases the weight of young poultry chicks, and reduces the toxicity of aconite.

[0035] The present invention preferably uses Schizophyllum and Acetobacter pasteurianum to ferment and pre-digest the aconite, controls the fermentation strains and fermentation culture conditions, and changes the original chemical components in the aconite extract through microbial fermentation, promotes the rapid accumulation of new effective active ingredients, and ultimately enhances the efficacy of the veterinary Sini Decoction, and also hydrolyzes highly toxic diester diterpenoid alkaloids into monoquinone alkaloids and alcoholamine alkaloids, thereby greatly reducing the acute toxicity of the veterinary Sini Decoction while ensuring the efficacy of the active ingredients.

[0036] The applicant found that by adding sucrose, corn syrup, vitamin H, potassium dihydrogen phosphate, ammonium citrate and magnesium carbonate, and adjusting the content of nutrients such as carbon source, nitrogen source, vitamins and so on in the culture medium, it is beneficial to the growth of mixed strains and the fermentation process of aconite, thereby promoting the conversion of toxic alkaloids in the fermentation mixture into harmless alkaloids.

[0037] The applicant also optimized the particle size of the aconite granules and the water content of the culture medium to be inoculated to provide adsorption and fermentation conditions for the mixed strains, thereby avoiding inhibiting the respiration of the mixed strains and thereby affecting the fermentation speed and effect of the mixed strains. Ultimately, through the fermentation of the mixed strains, the toxic effects of the veterinary Sini Decoction were significantly reduced, and a good synergistic effect was exerted.

[0038] Compared with the prior art, the present invention has the following beneficial effects:

[0039] (1) The veterinary Sini Decoction provided by the present invention has a high content of active ingredients, is not easy to precipitate, has high storage stability, and is almost non-toxic. When used for the prevention and health care of poultry and livestock, it can effectively improve the health of chicks, reduce the feed-to-meat ratio and the mortality rate, and improve the economic benefits of the farm.

[0040] (2) The present invention extracts the active ingredients from dried ginger and licorice by optimizing conditions, and improves the utilization rate of the raw materials of dried ginger and licorice by optimizing the concentration and alcohol extraction steps, and has a high content of effective active ingredients and better stability. By utilizing the characteristics of dried ginger extract that it is highly hot and non-toxic, and stays but does not move, it exerts the effects of warming yang, protecting the middle and returning yang to the meridians, and utilizes the synergistic effect of the combination of dried ginger and aconite to exert a better warming yang and meridians, significantly increasing the weight of young poultry chicks, while reducing the toxicity of aconite.

[0041] (3) The present invention preferably uses Schizophyllum and Acetobacter pasteurianum to ferment and pre-digest aconite. By controlling the fermentation strain and fermentation culture conditions, the original chemical composition of the aconite extract is changed, and the rapid accumulation of new effective active ingredients is promoted, thereby ultimately enhancing the efficacy of the veterinary Sini Decoction. The highly toxic diester diterpenoid alkaloids are also hydrolyzed and converted into monoquinone alkaloids and alcoholamine alkaloids. While ensuring the efficacy of the active ingredients, the acute toxicity of the veterinary Sini Decoction is greatly reduced.

[0042] (4) The present invention adds sucrose, corn syrup, vitamin H, potassium dihydrogen phosphate, ammonium citrate and magnesium carbonate, and adjusts the content of nutrients such as carbon source, nitrogen source, vitamins and the like in the culture medium, which is beneficial to the growth of the mixed strains and the fermentation process of aconite, thereby promoting the conversion of toxic alkaloids in the fermentation mixture into harmless alkaloids.

[0043] (5) The present invention provides adsorption and fermentation conditions for the mixed bacteria by optimizing the particle size of the aconite granules and the water content of the culture medium to be inoculated, thereby avoiding inhibiting the respiration of the mixed bacteria and further affecting the fermentation speed and effect of the mixed bacteria. Ultimately, through the fermentation of the mixed bacteria, the toxic effects of Sini Decoction for animals are significantly reduced, and a good synergistic effect is exerted. DETAILED DESCRIPTION

[0044] The present invention will be described below in conjunction with specific embodiments. It should be noted that the following embodiments are examples of the present invention and are only used to illustrate the present invention, but not to limit the present invention. Other combinations and various modifications within the concept of the present invention may be performed without departing from the spirit or scope of the present invention.

[0045] Unless otherwise specified, the following reagents can be easily obtained from commercial companies, among which Schizophyllum commune Fr. was purchased from the School of Life Sciences of Yantai University, Acetobacterium bakii was purchased from Ningbo Mingzhou Biotechnology Co., Ltd., and PDA slant medium and LB medium were purchased from Guangdong Huankai Microbiological Technology Co., Ltd.

[0046] Preparation Example 1

[0047] The cultivation steps of Schizophyllum commune Fr. are as follows:

[0048] In a sterile environment, the preserved Schizophyllum commune Fr. mother culture was inoculated into a 1 cm×1 cm bacterial block on a PDA slant medium, cultured at 25°C until the mycelium was full, and then inoculated into 500 mL of potato culture medium, and cultured statically at 25°C to obtain Schizophyllum commune Fr.

[0049] The steps for preparing potato culture medium are as follows: peel potatoes and cut them into pieces about 2 cm 3 Weigh 50 g of the small piece and soak it in 100 mL of deionized water for 15 min. Then transfer it to another 100 mL of deionized water and boil it for 20 min. Then sterilize it at 120°C and 85 kPa for 20 min and cool it naturally to obtain potato culture medium.

[0050] Preparation Example 2

[0051] The culture steps of Acetobacterium bakii are as follows:

[0052] In a sterile environment, the preserved Acetobacterium bakii mother culture was inoculated into a 1 cm × 1 cm bacterial block on LB medium, cultured at 26°C until the hyphae were full, and then inoculated into 500 mL of liquid culture medium, and cultured statically at 26°C to obtain Acetobacterium bakii.

[0053] The preparation steps of the liquid culture medium are as follows: 12 g yeast extract, 10 g tryptone, 8 g sodium chloride, 2 g potassium dihydrogen phosphate and 1 g magnesium sulfate are dissolved in 1000 mL deionized water, and sterilized at 121° C. and 0.02 kPa for 30 min to obtain a liquid culture medium.

[0054] Example 1

[0055] A method for preparing Sini Decoction for animals comprises the following steps:

[0056] (1) 150 g of dried ginger and 225 g of licorice root were mixed, 2850 mL of deionized water was added and boiled for 2 h, and the water solution and filter residue were filtered to obtain the water solution. 1500 mL of deionized water was added to the filter residue and boiled for 1.5 h, and the filter residue water solution was obtained after filtering. The water solution and the filter residue water solution were mixed to obtain a water extract, which was concentrated at a vacuum degree of -0.01 MPa and a temperature of 70°C for 6 h, and 2950 mL of 80% v / v ethanol solution was added. The mixture was allowed to stand for extraction at a temperature of 45°C for 3 days, and 0.05 wt% of needle-grade activated carbon was first added to treat for 20 min for coarse filtration to remove carbon, and then the filtrate was collected after passing through a 0.4 μm filter membrane to obtain a dried ginger and licorice root extract mixture;

[0057] (2) crushing the aconite root, passing it through a 40-mesh sieve to obtain aconite granules, taking 225 g of the aconite granules, adding 97 mL of deionized water to soak, adding 3.3 g of sucrose, 4.95 g of corn steep liquor, 0.1 g of vitamin H, 1 g of potassium dihydrogen phosphate, 0.26 g of ammonium citrate and 0.5 g of magnesium carbonate, adjusting the pH to 5, and then sterilizing at a temperature of 120° C. for 45 min to obtain a culture medium to be inoculated;

[0058] (3) Add 225 × 0.3 cm 2 Schizophyllum and 225 pieces × 0.2cm 2 Acetobacter pasteurianus, standing at 28°C for 8 days to obtain a fermentation mixture;

[0059] (4) The dried ginger and roasted licorice root extract mixture in step (1) and the fermented mixture in step (2) are mixed to obtain Sini Decoction for animals.

[0060] The Schizophyllum used was obtained from Preparation Example 1, and the Acetobacter pasteurianum was obtained from Preparation Example 2.

[0061] Example 2

[0062] A method for preparing Sini Decoction for animals comprises the following steps:

[0063] (1) 100 g of dried ginger and 150 g of roasted licorice root were mixed, 1875 mL of deionized water was added and boiled for 2 h, and the water solution and filter residue were obtained by filtering. 1000 mL of deionized water was added to the filter residue and boiled for 1.5 h, and the filter residue water solution was obtained after filtering. The water solution and the filter residue water solution were mixed to obtain a water extract, which was concentrated for 10 h at a vacuum degree of -0.01 MPa and a temperature of 60°C, and 1940 mL of 80% v / v ethanol solution was added. The mixture was allowed to stand for extraction at a temperature of 40°C for 4 days, and 0.05% needle-grade activated carbon was first added to treat for 20 min for coarse filtration to remove carbon, and then the filtrate was collected after filtering through a 0.3 μm filter membrane to obtain a dried ginger and roasted licorice root extract mixture;

[0064] (2) crushing the aconite root, passing it through a 10-mesh sieve to obtain aconite granules, taking 150 g of the aconite granules, adding 70 mL of deionized water to soak, adding 2.2 g of sucrose, 3.3 g of corn steep liquor, 0.07 g of vitamin H, 0.66 g of potassium dihydrogen phosphate, 0.18 g of ammonium citrate and 0.33 g of magnesium carbonate, adjusting the pH to 4, and then sterilizing at a temperature of 120° C. for 45 min to obtain a culture medium to be inoculated;

[0065] (3) Add 225 × 0.3 cm 2 Schizophyllum and 225 pieces × 0.2cm 2 Acetobacter pasteurianus, standing at 30°C for 5 days to obtain a fermentation mixture;

[0066] (4) The dried ginger and roasted licorice root extract mixture in step (1) and the fermented mixture in step (2) are mixed to obtain Sini Decoction for animals.

[0067] The Schizophyllum used was obtained from Preparation Example 1, and the Acetobacter pasteurianum was obtained from Preparation Example 2.

[0068] Example 3

[0069] A method for preparing Sini Decoction for animals comprises the following steps:

[0070] (1) 200 g of dried ginger and 300 g of licorice root were mixed, 3750 mL of deionized water was added and boiled for 2 h, and the water solution and filter residue were obtained by filtering. 2000 mL of deionized water was added to the filter residue and boiled for 1.5 h, and the filter residue water solution was obtained after filtering. The water solution and the filter residue water solution were mixed to obtain a water extract, which was concentrated for 2 h at a vacuum degree of -0.01 MPa and a temperature of 80°C, and 3885 mL of 80% v / v ethanol solution was added. The mixture was allowed to stand for extraction at a temperature of 50°C for 4 days, and 0.05% needle-grade activated carbon was first added to treat for 20 min for coarse filtration to remove carbon, and then the filtrate was collected after filtering through a 0.5 μm filter membrane to obtain a dried ginger and licorice root extract mixture;

[0071] (2) crushing the aconite root and passing it through a 60-mesh sieve to obtain aconite granules, taking 300 g of the aconite granules and adding 135 mL of deionized water to soak, adding 4.35 g of sucrose, 6.53 g of corn steep liquor, 0.13 g of vitamin H, 1.3 g of potassium dihydrogen phosphate, 0.35 g of ammonium citrate and 0.65 g of magnesium carbonate, adjusting the pH to 6, and then sterilizing at a temperature of 120° C. for 45 min to obtain a culture medium to be inoculated;

[0072] (3) Add 225 × 0.3 cm 2 Schizophyllum and 225 pieces × 0.2cm 2 Acetobacter pasteurianus, standing at 20°C for 10 days to obtain a fermentation mixture;

[0073] (4) The dried ginger and roasted licorice root extract mixture in step (1) and the fermented mixture in step (2) are mixed to obtain Sini Decoction for animals.

[0074] The Schizophyllum used was obtained from Preparation Example 1, and the Acetobacter pasteurianum was obtained from Preparation Example 2.

[0075] Example 4

[0076] A method for preparing Sini Decoction for animals, the specific implementation method is the same as that of Example 1, except that 225 pieces of 0.5 cm 2 Schizophyllum and 225 pieces × 0.2cm 2 Acetobacter pasteurianus.

[0077] Example 5

[0078] A method for preparing Sini Decoction for animals, the specific implementation method is the same as that of Example 1, except that 225 pieces of 0.3 cm 2 Acetobacter pasteurianus and 225 pieces × 0.3cm 2 Schizophyllum.

[0079] Example 6

[0080] A method for preparing Sini Decoction for animals, the specific implementation method is the same as that of Example 1, except that the particle size of the aconite granules is 5 mesh.

[0081] Example 7

[0082] A method for preparing Sini Decoction for animals, the specific implementation method is the same as that of Example 1, except that the particle size of the aconite granules is 70 mesh.

[0083] Example 8

[0084] A method for preparing Sini Decoction for animals, the specific implementation method is the same as that of Example 1, except that 106 mL of deionized water is added in step (2).

[0085] Comparative Example 1

[0086] A method for preparing Sini Decoction for animals, the specific implementation method is the same as that of Example 1, except that 225 pieces of 0.3 cm 2 Schizophyllum and 0 Acetobacter pasteurianum.

[0087] Comparative Example 2

[0088] A method for preparing Sini Decoction for animals, the specific implementation method is the same as that of Example 1, except that 0 pieces of Schizophyllum and 225 pieces of 0.2 cm 2 Acetobacter pasteurianus.

[0089] Performance test of Sini Decoction for animals:

[0090] The following performance tests were performed on the animal Sini Decoction prepared in the above examples and comparative examples. The specific test results are shown in Table 1:

[0091] (1) Stability test: The animal Sini Decoction prepared in the embodiment and the comparative example was stored at a temperature of 40±2°C and a humidity of 75±5% for 6 months, and the stability test of the animal Sini Decoction was performed to observe the time when precipitation appeared. The later the precipitation appeared or no precipitation appeared during the test period, the higher the stability of the animal Sini Decoction finished product and the better the quality;

[0092] (2) Test for content of diester alkaloids: mixed standard solutions of diester alkaloids (aconitine, mesaconitine and mesaconitine) were prepared with concentrations of 12.096 μg / mL, 12.320 μg / mL and 12.384 μg / mL, respectively. 50 μL was taken and diluted to a gradient of 100 μL, 200 μL, 300 μL, 400 μL, 500 μL and 1000 μL, respectively, and liquid chromatography was performed under the following conditions: C18 column, mobile phase was buffer (0.2 wt % glacial acetic acid adjusted to pH 6.20 with triethylamine) and acetonitrile, gradient elution, flow rate 1.0 mL / min, injection volume 20 μL, detection wavelength 240 nm, column temperature 30°C; standard curve equations of aconitine, mesaconitine and mesaconitine were y=17.348x+22.303, R 2 =0.9996; y=18.39x+47.585, R 2 =0.9995; y=19.089x+12.128, R 2=0.9999. The Sini Decoction for Animal Use prepared in Examples 1 to 8, Comparative Example 1 and Comparative Example 2 was tested for diester-type alkaloid content according to the above-mentioned liquid chromatography test conditions.

[0093] Table 1

[0094] project Stability / day Diester alkaloid content / μg / g Example 1 No precipitation 151 Example 2 No precipitation 157 Example 3 No precipitation 163 Example 4 No precipitation 172 Example 5 No precipitation 180 Example 6 No precipitation 187 Example 7 7 185 Example 8 16 191 Comparative Example 1 20 193 Comparative Example 2 30 197

[0095] As shown in Table 1, the animal Sini Decoction provided by Examples 1 to 3 of the present invention has a high content of active ingredients, is not easy to precipitate, has high storage stability, and is almost non-toxic. When used for the prevention and health care of poultry and livestock, it can effectively improve the health of young chicks, reduce the feed-to-meat ratio and the mortality rate, and improve the economic benefits of the farm. Example 4 and Example 5 changed the inoculation amount of the strain, and Comparative Example 1 and Comparative Example 2 used a single strain to ferment the aconite, and the fermentation effect was reduced, especially the accumulation and conversion of effective active ingredients were affected, resulting in different degrees of reduction in the stability of the animal Sini Decoction, and the conversion of toxic substances was not ideal; Example 6 and Example 7 changed the particle size of the aconite particles, and Example 8 changed the water content of the culture medium to be inoculated, which affected the fermentation process of the composite strain from another aspect, and finally had an adverse effect on the stability of the animal Sini Decoction and the content of diester-type alkaloids.

[0096] Animal experiments on Sini Decoction for veterinary use:

[0097] 500 817 broiler chickens aged 1 day were selected and divided into two groups, each group had 250 chickens, one group was a blank group, and the other group was a veterinary Sini Decoction group. The blank group did not use veterinary Sini Decoction. The veterinary Sini Decoction used in the veterinary Sini Decoction group was prepared as in Example 1, and the dosage was based on the national standard for the dosage of Sini Decoction for poultry, 0.6 mL per kilogram of body weight, and the medication was used continuously for 5 days. The two groups of chickens were fed according to conventional feeding management and immunization procedures, and the clinical symptoms of the chickens were observed every day, and the number of deaths was counted to calculate the mortality rate. The chickens were weighed at 7 days of age, the feed intake was counted, and the feed-to-meat ratio was calculated. The specific experimental results are shown in Table 2:

[0098] Table 2

[0099] Group Weight at 7 days old / g Feed to meat ratio mortality rate / % Blank group 125.52 1.31 1.38 Veterinary Sini Decoction Group 129.60 1.08 0.97

[0100] As can be seen from Table 2, compared with the blank group, the veterinary Sini Decoction group preferably prepared by the present invention has an increase in the weight of chickens at 7 days of age by 3.25%, a decrease in the feed-to-meat ratio by 17.56%, and a decrease in the mortality rate by 0.41%. This indicates that the veterinary Sini Decoction preferably prepared by the present invention can increase the weight of chicks, reduce the meat-to-feed ratio, ensure the health of chicks, and reduce the mortality rate.

[0101] The above description is only a preferred embodiment of the present invention and does not limit the present invention in any form. Although the present invention has been disclosed as a preferred embodiment as above, it is not used to limit the present invention. Any technician familiar with this profession can make some changes or modifications to equivalent embodiments of equivalent changes using the technical contents disclosed above without departing from the scope of the technical solution of the present invention. However, any simple modification, equivalent change and modification made to the above embodiments based on the technical essence of the present invention without departing from the content of the technical solution of the present invention still fall within the scope of the technical solution of the present invention.

Claims

1. A method for preparing Sini Decoction for animals, characterized in that: The preparation method comprises the following steps: (1) boiling a mixture of dried ginger and roasted licorice to obtain a water extract, and concentrating, alcohol extracting and filtering to obtain a dried ginger and roasted licorice extract mixture; (2) crushing and sieving the aconite root to obtain aconite root granules, taking the aconite root granules and soaking them in deionized water, adding sucrose, corn syrup, vitamin H, potassium dihydrogen phosphate, ammonium citrate and magnesium carbonate, adjusting the pH, and sterilizing to obtain a culture medium to be inoculated; (3) adding the mixed strains to the culture medium to be inoculated in step (2), allowing the mixture to ferment to obtain a fermentation mixture; (4) The dried ginger and roasted licorice root extract mixture in step (1) and the fermented mixture in step (2) are mixed to obtain Sini Decoction for animals.

2. The method for preparing the veterinary Sini Decoction according to claim 1, characterized in that: The concentration operation is as follows: at a vacuum degree of -0.01 to 0 MPa and a temperature of 60 to 80° C., the concentration is performed for 2 to 10 hours to obtain a concentrate.

3. The method for preparing the veterinary Sini Decoction according to claim 1, characterized in that: The alcohol extraction process is as follows: adding 70-90% v / v alcohol solution, extracting at a temperature of 40-50° C. for 2-4 days.

4. The method for preparing Sini Decoction for animals according to claim 1, characterized in that: The particle size of the aconite granules in step (2) is 10 to 60 meshes.

5. The method for preparing Sini Decoction for animals according to claim 1, characterized in that: The amount of deionized water added in step (2) is 40-45% of the mass of the aconite granules.

6. The method for preparing Sini Decoction for animals according to claim 1, characterized in that: In the step (2), the added amounts of sucrose, corn syrup, vitamin H, potassium dihydrogen phosphate, ammonium citrate and magnesium carbonate are 0.5-1.5%, 1-2%, 0.01-0.05%, 0.2-0.3%, 0.01-0.15% and 0.1-0.2% of the total mass of the aconite granules and deionized water, respectively.

7. The method for preparing Sini Decoction for animals according to claim 1, characterized in that: The fermentation conditions are: temperature 20-30° C., pH 6-7, and fermentation 5-10 days.

8. The method for preparing Sini Decoction for animals according to claim 1, characterized in that: The mixed bacterial species include Schizophyllum and Acetobacter pasteurianum.

9. The method for preparing Sini Decoction for animals according to claim 8, characterized in that: The amount of the schizophyllum added is 0.1 to 0.4 cm 2 Schizophyllum / g Aconite granules, the amount of Acetobacter pasteurianum added is 0.1~0.2cm 2 Acetobacter pasteurianus / g of Aconitum granules.

10. A Sini Decoction for animals obtained by the preparation method according to any one of claims 1 to 9, characterized in that: The veterinary Sini Decoction comprises the following raw materials in parts by weight: 100-200 parts of dried ginger, 150-300 parts of roasted licorice root and 150-300 parts of aconite root.