Centrifugal bag

Abstract

The invention provides a centrifugal bag. The centrifugal bag comprises a bag body, a connecting piece and a mounting hole. The bag body is formed by sealing a first film layer and a second film layer. The connecting piece is communicated with the bag body and comprises a first connecting part and a second connecting part, and a notch is formed in the end, located in the bag body, of the first connecting part. The mounting hole penetrates through the bag body in the thickness direction and is used for mounting the centrifugal bag in centrifugal equipment. According to the centrifugal bag, the notch is formed in the connecting piece, and the mounting hole is formed in the bag body, so that the situation that a sample is not easy to discharge due to the fact that the through hole of the connecting piece is sealed (sucked) during liquid discharge can be prevented, and meanwhile liquid in the bag body in centrifugal equipment is promoted to be completely discharged. The centrifugal bag is simple in structure, low in cost and capable of treating samples large in size at a time.

Description

Technical Field

[0001] The present application relates to the field of separation technology, and in particular to a centrifuge bag. Background Art

[0002] In the fields of bioengineering, cell processing, etc., it is usually necessary to sort and purify samples such as cell fluids, and sorting and purification are generally completed by centrifugation. In the industrialization stage, operations such as sorting and purification require the use of automated equipment in conjunction with disposable pipeline consumables to avoid possible mistakes and contamination risks during manual operation. Disposable pipeline consumables include centrifuge containers. Most of the existing centrifuge containers at home and abroad are in the form of centrifuge cups or centrifuge bags. However, the existing centrifuge cups or centrifuge bags have complex structures and manufacturing processes, high costs, and a small volume of samples processed at one time. In addition, when squeezing or extracting samples from existing centrifuge bags, the outlet part is easily blocked or the sample is not completely extruded. Summary of the invention

[0003] In view of this, the present application proposes a centrifuge bag to solve at least one of the above problems.

[0004] The present application proposes a centrifuge bag for holding a sample and placing it in a centrifugal device. The centrifuge bag includes a bag body, a connector and a mounting hole. The bag body is formed by sealing a first film layer and a second film layer. The connector is in communication with the bag body, and the connector includes a first connecting portion and a second connecting portion that are connected to each other. At least a portion of the first connecting portion is accommodated in the bag body, and a notch is formed at the end of the first connecting portion located in the bag body. The second connecting portion is fixed to the surface of the first film layer that is away from the second film layer. The mounting hole penetrates the bag body in the thickness direction, and the mounting hole is configured to install the centrifuge bag in a centrifugal device.

[0005] In one embodiment, the centrifuge bag further comprises an anti-absorption sheet, wherein the anti-absorption sheet is disposed on the surface of the second film layer and corresponds to the connecting piece, and the hardness of the anti-absorption sheet is greater than the hardness of the second film layer.

[0006] In one embodiment, the anti-absorption sheet is disposed on the surface of the second film layer away from the first film layer. Alternatively, the anti-absorption sheet is disposed on the surface of the second film layer close to the first film layer.

[0007] In one embodiment, the anti-absorption sheet includes an arc-shaped portion and an extension portion connected to an edge of the arc-shaped portion, the arc-shaped portion is recessed in a direction away from the notch, and the extension portion is arranged corresponding to the second connecting portion.

[0008] In one embodiment, the edge of the mounting hole has a first sealing portion, and the first sealing portion is formed by sealing the first film layer and the second film layer located at the edge of the mounting hole.

[0009] In one embodiment, the bag body has a second sealing portion, which is arranged around the first sealing portion and is formed by sealing a portion of the first film layer and the second film layer.

[0010] In one embodiment, the centrifuge bag further comprises a sample tube. The sample tube is connected to an end of the first connection portion away from the notch and is in communication with the interior of the bag body through the first connection portion.

[0011] In one embodiment, the bag body includes a first area and a second area that are connected. The second area is connected to an edge of the first area, the connecting member and the mounting hole are provided in the first area, and the second area is used to accommodate the sample after centrifugation.

[0012] In one embodiment, there are a plurality of mounting holes, and the plurality of mounting holes are arranged around the connecting member with the connecting member as the center.

[0013] In one embodiment, the bag body and the connecting piece are each independently made of one or more of polyvinyl chloride, polyethylene, ethylene-vinyl acetate copolymer and thermoplastic polyurethane.

[0014] The centrifugal bag of the present application, by providing a notch on the connecting piece and a mounting hole on the bag body, can prevent the through hole of the connecting piece from being blocked (sucked to death) during liquid discharge, thereby making it difficult to discharge the sample, and at the same time, promotes the complete discharge of the liquid in the bag body in the centrifugal device. The centrifugal bag of the present application has a simple structure and low cost, and can process a large volume of sample at one time. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 A schematic diagram of the structure of a centrifuge bag provided in one embodiment of the present application.

[0016] Figure 2 for Figure 1 A top view of a centrifuge bag is shown.

[0017] Figure 3 for Figure 1 Cross-sectional view along III-III.

[0018] Figure 4 for Figure 3 A in the figure is an enlarged schematic diagram of an embodiment.

[0019] Figure 5 for Figure 3A in FIG. 1 is an enlarged schematic diagram of another embodiment.

[0020] Figure 6 for Figure 1 Schematic diagram of the structure of the connecting piece of the centrifuge bag shown.

[0021] Figure 7 A schematic structural diagram of a centrifuge bag provided in another embodiment of the present application.

[0022] Figure 8 for Figure 7 A top view of a centrifuge bag is shown.

[0023] Fig. 9 This is a schematic diagram of the centrifugal separation of hematopoietic stem cells in Example 1 of the present application.

[0024] Fig.10 This is a schematic diagram of the separation and purification of mononuclear cells in Example 2 of the present application.

[0025] Main component symbols

[0026] Centrifuge Bag 100

[0027] Bag body 10

[0028] Connector 20

[0029] Anti-sucking sheet 30

[0030] Sample tube 40

[0031] The first film layer 11

[0032] The second film layer 12

[0033] Mounting holes 110

[0034] The first sealing portion 111

[0035] Second sealing portion 112

[0036] First Area 101

[0037] Second area 102

[0038] First connection portion 21

[0039] The second connection portion 22

[0040] Through hole 210

[0041] The first end portion 211

[0042] The second end portion 212

[0043] Gap 213

[0044] Step 214

[0045] Arc portion 31

[0046] Extension 32

[0047] The following specific implementation manner will further illustrate the embodiments of the present application in conjunction with the above-mentioned drawings. DETAILED DESCRIPTION

[0048] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art of the technical field of the present application. The terms used herein are only for the purpose of describing specific implementation methods and are not intended to limit the present application embodiments.

[0049] It should be noted that all directional indications in the embodiments of the present application (such as up, down, left, right, front, back, etc.) are only used to explain the relative position relationship, movement status, etc. between the components under a certain specific posture (as shown in the accompanying drawings). If the specific posture changes, the directional indication will also change accordingly.

[0050] Here, the embodiments of the present application are described with reference to the cross-sectional views, which are schematic diagrams of the idealized embodiments (and intermediate configurations) of the present application. Thus, it is foreseeable that the shapes of the diagrams are different due to manufacturing processes and / or tolerances. Therefore, the embodiments of the present application should not be interpreted as being limited to the specific shapes of the regions illustrated here, but should include deviations in shapes such as those produced due to manufacturing. The regions shown in the figures are themselves only schematic, and their shapes are not intended to illustrate the actual shapes of the devices, and are not intended to limit the scope of the present application.

[0051] It should be noted that when a component is referred to as being "fixed to" or "mounted on" another component, it may be directly on the other component or there may be a central component. When a component is considered to be "set on" another component, it may be directly set on the other component or there may be a central component at the same time. The term "and / or" as used herein includes all and any combinations of one or more of the relevant listed items.

[0052] Some embodiments of the present application are described in detail below in conjunction with the accompanying drawings. In the absence of conflict, the following embodiments and features in the embodiments can be combined with each other.

[0053] See also Figures 1 to 3The present application provides a centrifuge bag 100, which includes a bag body 10, a connector 20 and a mounting hole 110. The centrifuge bag 100 is used to hold a sample (not shown) and place it in a centrifuge device (not shown), so as to separate the sample into at least two components, and then separate the sample into different layers and finally enter different containers. The sample can be a biological sample, which can be but not limited to blood, bone marrow aspirate, leukocyte apheresis sample, cell or composition containing cell or cell component, etc.

[0054] See also Figure 1 and Figure 2 , the mounting hole 110 passes through the bag body 10 in the thickness direction, and is used to install the centrifuge bag 100 in the centrifugal equipment to complete the centrifugal operation. The number of mounting holes 110 can be one or more, and the present application is not limited. Multiple mounting holes 110 can be evenly spaced around the connector 20 with the connector 20 as the center, and the connector 20 is located in the area surrounded by multiple mounting holes 110. The spacing between the mounting holes 110 can be slightly larger to prevent the upper and lower membrane layers of the bag body 10 from being sucked to death when the pump draws the sample. In the present embodiment, four mounting holes 110 are evenly spaced around the connector 20. The mounting holes 110 can be regular or irregular shaped holes such as circular holes and triangular holes, and their sizes can also be adjusted according to actual needs, and the present application is not limited.

[0055] See also Figure 3 and Figure 4 The bag body 10 is formed by sealing a first film layer 11 and a second film layer 12. The first film layer 11 and the second film layer 12 may be in a circular shape but not limited thereto, and the edges of the first film layer 11 and the second film layer 12 may be sealed by a heat-sealing process (using heat to make the material reach a temperature at which it can be deformed, and then combining two or more materials together by pressure) but not limited thereto, so that the cavity formed by the sealing of the first film layer 11 and the second film layer 12 may be used as a receiving space for accommodating the sample.

[0056] See also Figures 4 to 6The connector 20 is connected to the receiving space inside the bag body 10. The connector 20 includes a first connecting portion 21 and a second connecting portion 22 connected to each other. The second connecting portion 22 can be arranged around the outer periphery of the first connecting portion 21. The first connecting portion 21 can penetrate the central area of ​​the second connecting portion 22 and extend from the second connecting portion 22. At least part of the first connecting portion 21 is accommodated in the bag body 10. The first connecting portion 21 has a through hole 210 penetrating the first connecting portion 21 along the height direction (i.e., the thickness direction of the bag body 10), so that the first connecting portion 21 can communicate with the inside of the bag body 10. The first connecting portion 21 has a first end 211 located inside the bag body 10 and a second end 212 located outside the bag body 10. The second end 212 is arranged opposite to the first end 211. A part of the surface of the first end 211 is recessed in a direction close to the second end 212 to form a notch 213, and the notch 213 is located inside the bag body 10. The second connecting portion 22 is fixed to the surface of the first film layer 11 away from the second film layer 12.

[0057] By providing the notch 213 on the connector 20 , when the sample in the bag body 10 is discharged by squeezing the bag body 10 or by suction through a pump, due to the presence of the notch 213 , the second film layer 12 will not seal the through hole 210 of the connector 20 , and the sample can be discharged through the notch 213 and then through the through hole 210 .

[0058] See also Figure 4 and Figure 5 In some embodiments, the centrifuge bag 100 further includes an anti-absorption sheet 30. The anti-absorption sheet 30 is disposed on the surface of the second film layer 12 and is disposed corresponding to the connector 20. The corresponding arrangement means that the orthographic projection of the anti-absorption sheet 30 at least partially overlaps with the orthographic projection of the connector 20. Figure 4 As shown, the anti-absorption sheet 30 can be disposed on the surface (outer surface) of the second film layer 12 away from the first film layer 11 by a heat-sealing process. Figure 5 As shown, the anti-absorption sheet 30 can be disposed on the surface (inner surface) of the second film layer 12 close to the first film layer 11 through a heat-sealing process. The hardness of the anti-absorption sheet 30 is greater than that of the second film layer 12, and the anti-absorption sheet 30 is not easily deformed when subjected to external force. There is a gap between the anti-absorption sheet 30 and the notch 213, that is, the anti-absorption sheet 30 itself cannot completely fit with the notch 213, and can also prevent the second film layer 12 from completely fitting with the notch 213. The notch 213 and the anti-absorption sheet 30 cooperate to better prevent the through hole 210 of the connector 20 from being sealed (sucked to death) when the sample is discharged, so that the sample cannot be completely discharged.

[0059] In some embodiments, when the anti-absorption sheet 30 is located on the inner surface, even if the outer second film layer 12 is squeezed externally and the squeeze is transferred to the anti-absorption sheet 30, the anti-absorption sheet 30 can maintain a certain shape when squeezed and does not completely fit the inner wall of the notch 213, and there is still a gap between the anti-absorption sheet 30 and the notch 213 (this gap can be the gap of the notch 213 itself, or it can be a gap formed when the anti-absorption sheet 30 and the second end 212 are not in contact).

[0060] In some embodiments, when the anti-absorption sheet 30 is located on the outer surface, due to the high hardness of the anti-absorption sheet 30, even if the anti-absorption sheet 30 is subjected to external pressure, it can maintain a certain shape without transferring the pressure to the second film layer 12, and there is still a gap between the anti-absorption sheet 30 (the second film layer 12) and the notch 213 (this gap can be the gap of the notch 213 itself, or it can be a gap formed when the second film layer 12 and the second end 212 are not in contact).

[0061] like Figure 4 and Figure 5 As shown, in some embodiments, the anti-absorption sheet 30 includes an arc portion 31 and an extension portion 32 connected to the edge of the arc portion 31. That is, the central area of ​​the anti-absorption sheet 30 is the arc portion 31, and the edge area is the extension portion 32. The arc portion 31 can be arranged corresponding to the first connecting portion 21 and part of the second connecting portion 22, that is, the orthographic projection of the first connecting portion 21 and part of the second connecting portion 22 can cover the orthographic projection of the arc portion 31. The arc portion 31 is a curved surface with a certain curvature, and the arc portion 31 is concave in a direction away from the notch 213. The extension portion 32 is a plane, which can be arranged corresponding to the second connecting portion 22. The extension portion 32 is arranged corresponding to the second connecting portion 22, which means that the orthographic projection of the extension portion 32 overlaps with the orthographic projection of the second connecting portion 22 at least partially. When the bag body 10 is squeezed or sucked multiple times, the extension portion 32 can avoid sharp contact between the arc portion 31 and the second film layer 12, and reduce the damage caused by the arc portion 31 to the second film layer 12.

[0062] In some embodiments, Figure 1 and Figure 2As shown, the edge of the mounting hole 110 has a first sealing portion 111. The first sealing portion 111 is formed by sealing the first film layer 11 and the second film layer 12 located at the edge of the mounting hole 110. Specifically, the first sealing portion 111 can be formed by a heat sealing process, that is, the first film layer 11 and the second film layer 12 located at the edge of the mounting hole 110 are hot-melted and then pressed together to form the first sealing portion 111. After heat sealing, the side wall of the mounting hole 110 is the first film layer 11 and the second film layer 12 after hot-melt pressing. In this embodiment, the mounting hole 110 is a circular hole, and the first sealing portion 111 has a certain width along the radial direction of the mounting hole 110. That is, the first sealing portion 111 is roughly annular. The first sealing portion 111 is used to seal the mounting hole 110 to prevent the sample in the bag body 10 from flowing out through the mounting hole 110.

[0063] Furthermore, if Figure 1 and Figure 2 As shown, the bag body 10 may also have a second sealing portion 112. The second sealing portion 112 is arranged around the first sealing portion 111, and the second sealing portion 112 is located at the periphery of the first sealing portion 111. The second sealing portion 112 may be formed by sealing a portion of the first film layer 11 and the second film layer 12 through a heat sealing process. The second sealing portion 112 may be a regular shape or an irregular shape such as a triangle or a trapezoid, and the present application does not limit it. If the first sealing portion 111 is damaged, the second sealing portion 112 can prevent the sample in the bag body 10 from flowing out, and the second sealing portion 112 can further strengthen the sealing at the mounting hole 110.

[0064] like Figure 7 and Figure 8 As shown, in some embodiments, the second sealing portion 112 may also be omitted.

[0065] like Figures 1 to 5 As shown, in some embodiments, the centrifuge bag 100 further includes a sample tube 40. The sample tube 40 is a liquid inlet and outlet tube, and the sample can enter the bag body 10 through the sample tube 40, and the sample can also be led out of the bag body 10 through the sample tube 40. Figure 4 and Figure 5 As shown, the sample tube 40 is connected to the end of the first connection part 21 away from the notch 213, that is, connected to the second end 212. The sample tube 40 is connected to the interior of the bag body 10 through the first connection part 21. In some embodiments, the sample tube 40 can be bonded to the through hole 210 of the first connection part 21 through an adhesive layer (not shown). In this embodiment, the diameter of the portion of the through hole 210 close to the second end 212 is smaller than the diameter of the portion of the through hole 210 close to the first end 211. A step portion 214 is formed inside the through hole 210 at the location where the aperture changes, and the sample tube 40 can be inserted into the step portion 214.

[0066] Furthermore, the sample tube 40 may be a soft tube, and its material may be but not limited to polyvinyl chloride (PVC), polyethylene (PE), ethylene-vinyl acetate copolymer (EVA), thermoplastic polyurethane (TPU), etc.

[0067] like Figures 1 to 5 As shown, in some embodiments, one bag body 10 may have only one sample tube 40. In other implementations, one bag body 10 may be provided with multiple sample tubes 40, which may be used for different processing steps of one sample. In other embodiments, one bag body 10 may be divided into multiple independent sub-bag bodies (not shown), each of which may be provided with one or more sample tubes, and the sub-bag bodies are not connected to each other, so as to be used for processing multiple samples at one time.

[0068] like Figure 1 , Fig. 9 and Fig.10 As shown, in some embodiments, the bag body 10 includes a first area 101 and a second area 102 that are connected, and the second area 102 is connected to the edge of the first area 101. In this embodiment, the bag body 10 is roughly circular, the first area 101 is roughly the central area of ​​the circle, and the second area 102 is roughly annular. The connector 20 and the anti-absorption sheet 30 are arranged in the first area 101, and the mounting hole 110, the first sealing portion 111 and the second sealing portion 112 are located in the first area 101. The height inside the second area 102 can be higher than the height inside the first area 101, and the second area 102 is used to accommodate the sample after centrifugation.

[0069] Further, the internal volume of the bag body 10 can be 10mL to 2000mL. Further, the internal volume of the bag body 10 can be 200mL to 1000mL. The diameter of the bag body 10 can be 50mm to 300mm, and the thickness of the first film layer 11 and the second film layer 12 can be the same, for example, the thickness of the first film layer 11 or the second film layer 12 can be 0.3mm. In other embodiments, the thickness of the first film layer 11 and the second film layer 12 can also be different. The material of the bag body 10 can be but not limited to polyvinyl chloride, polyethylene, ethylene-vinyl acetate copolymer, thermoplastic polyurethane, etc.

[0070] In some embodiments, Figure 6 As shown, the first connection part 21 is roughly cylindrical, and the second connection part 22 is roughly disc-shaped. The second connection part 22 can be fixed to the surface of the second film layer 12 away from the first film layer 11 by a heat-sealing process, and the diameter of the second connection part 22 can be 26 mm. The connecting member 20 can be a flange. In this embodiment, the number of notches 213 is two, and the shape of the notches 213 is semicircular. The two notches 213 are symmetrically arranged (due to the viewing angle, Figure 6In other embodiments, the shape of the notch 213 may be a regular or irregular shape such as a square or a triangle, and the number of the notch 213 may be one or more than two.

[0071] Furthermore, the material of the connecting member 20 may be, but is not limited to, polyvinyl chloride, polyethylene, ethylene-vinyl acetate copolymer, thermoplastic polyurethane, etc.

[0072] In some embodiments, the shape of the anti-absorption sheet 30 may be circular, larger than the diameter of the second connecting portion 22, for example, the diameter of the anti-absorption sheet 30 may be 29 mm. In other embodiments, the shape of the anti-absorption sheet 30 may also be square, rectangular, etc., which is not limited in the present application. In other embodiments, the thickness of the anti-absorption sheet 30 is greater than the thickness of the first film layer 11 or the second film layer 12, for example, the thickness of the anti-absorption sheet 30 may be 0.4 mm.

[0073] Furthermore, the material of the anti-absorption sheet 30 may be, but is not limited to, polyvinyl chloride (PVC). It is understandable that PVC may have different hardnesses, and even if the anti-absorption sheet 30 and the bag body 10 are both made of PVC, the hardnesses of the two are different. The hardness of the PVC used for the anti-absorption sheet 30 is greater than the hardness of the PVC used for the bag body 10.

[0074] The centrifuge bag 100 of the present application can be used in the following processes: separating a sample into layers with different densities, and discharging these layers from the centrifuge bag 100 into different containers, for example, separating blood or bone marrow into different components; discharging unnecessary components in the sample into specific containers by centrifugation to achieve the purpose of enriching and purifying the required components; separating cells by adding additives such as Ficoll or Percoll to provide density gradient preparation; after the sample is separated into specific layers by centrifugation, adding magnetic beads for mixing, incubation, etc.

[0075] The present application will be further described below in conjunction with specific embodiments.

[0076] Example 1

[0077] Complete hematopoietic stem cell sorting from peripheral blood, such as CD34 + Hematopoietic stem cells, used for research or cell gene therapy.

[0078] Inject 50mL to 600mL of peripheral blood sample into Figure 1 The centrifuge is started and a centrifuge chamber of the centrifuge is used to apply a centrifugal force of 200 g to the centrifuge bag for about 15 minutes. The sample is separated into multiple layers including red blood cells, white blood cells and plasma (buffer) in the centrifuge bag, such as Fig. 9The outer layer is composed of red blood cells, the middle is composed of white blood cells, and the white blood cells (including CD34 + Hematopoietic stem cells) form a thin white ring adjacent to the red blood cell layer, and the inner layer is composed of the yellow plasma portion.

[0079] The centrifugal bag (anti-absorption sheet or second membrane layer) is squeezed from below by the squeezing structure of the centrifugal chamber, and the centrifugal bag is deformed, and the volume inside the bag body becomes smaller, so that the plasma layer is discharged into a specific container through the sample tube. After that, the buffer is introduced into the centrifugal bag to wash the sample therein. The centrifugal chamber applies a centrifugal force of 200g to the centrifugal bag for about 15 minutes, and the sample in the centrifugal bag is again divided into a red blood cell layer, a white blood cell layer, and a buffer layer. The centrifugal chamber squeezes the centrifugal bag again to exclude the buffer layer. It can be washed multiple times.

[0080] After that, specific antibodies and specific magnetic beads were added to the centrifuge bag for incubation to detect CD34 + The hematopoietic stem cells are specifically labeled, and then the centrifuged samples in the centrifuge bag are transferred to a specific container through squeezing and pumping in the centrifuge chamber, and then CD34 is specifically selected through magnetic separation. + Hematopoietic stem cells. CD34 + Hematopoietic stem cells are returned to the human body after a series of operations such as lentiviral transduction to achieve the purpose of treating the disease.

[0081] Example 2

[0082] Mononuclear cells (PBMC) are isolated and purified from peripheral blood collected from donors for research and storage.

[0083] First, introduce 100 mL of separation solution into the centrifuge bag, start the centrifuge, apply a centrifugal force of about 200 g to the centrifuge bag through the centrifugal chamber of the centrifuge, then introduce about 200 mL of sample into the centrifuge bag, and then apply a centrifugal force of 200 g to the centrifuge bag for about 10 minutes. The sample will be separated into multiple layers including red blood cells, separation solution, white blood cells and plasma in the centrifuge bag, such as Fig.10 As shown, the outer layer is composed of the red blood cell part, the separation liquid layer is between the red blood cell layer and the white blood cell layer, the white blood cell is a thin white ring, and the inner layer is composed of the yellow plasma part (buffer).

[0084] After that, the centrifugal bag is squeezed from below by the squeezing structure of the centrifugal chamber, and the centrifugal bag is deformed, and the volume inside the bag body becomes smaller, so that the plasma layer, the white blood cell layer, and the separation liquid layer are discharged into the specific container 1. Then, the red blood cell layer is discharged into the specific container 2 by squeezing and pumping. After that, the buffer is introduced into the centrifugal bag, and the centrifugal chamber drives the centrifugal bag to repeatedly rotate forward and reverse, and then the liquid in the centrifugal bag is discharged by squeezing and pumping, and the centrifugal bag is cleaned. The cleaning times can be multiple times.

[0085] After the centrifuge bag is cleaned, the liquid containing mononuclear cells in the specific container 1 is introduced into the centrifuge bag, and the centrifugal chamber of the centrifuge again applies a centrifugal force of 200g for about 15 minutes. At this time, the sample in the centrifuge bag forms a white blood cell layer and a plasma layer, and the centrifuge bag is squeezed by the squeezing structure of the centrifuge chamber to remove most of the plasma layer; the freezing liquid is then introduced into the centrifuge bag, and then a centrifugal force of 200g is applied to the centrifuge bag for about 10 minutes, and the freezing liquid is expelled by squeezing the squeezing structure of the centrifuge chamber and pumping, and the volume in the centrifuge bag is adjusted to about 5mL, and it is pumped out to the specific container 3; 10mL of freezing liquid is added to the centrifuge bag, and the centrifugal chamber drives the centrifuge bag to repeatedly reverse forward and backward to resuspend the cells in the centrifuge bag, and then it is pumped out to the specific container 3 by pumping, completing the separation and purification of mononuclear cells.

[0086] The centrifuge bag 100 of the present application, by providing a notch 213 on the connector 20 and providing a mounting hole 101 on the centrifuge bag 100, can prevent the through hole 210 of the connector 20 from being blocked (sucked to death) during liquid discharge, thereby preventing the sample from being difficult to discharge, and at the same time, promotes complete discharge of the liquid in the centrifuge bag 100 in the centrifugal device. The centrifuge bag of the present application has a simple structure and low cost, and can process a large volume of sample at one time.

[0087] The above descriptions are some specific implementations of the present application, but they are not limited to these implementations in actual application. For ordinary technicians in this field, other variations and changes made according to the technical concept of the present application should all fall within the protection scope of the present application.

Claims

1. A centrifuge bag for holding a sample and placing it in a centrifuge device, characterized in that: The centrifuge bag comprises: The bag body is formed by sealing the first film layer and the second film layer; a connecting member connected to the bag body, the connecting member comprising a first connecting portion and a second connecting portion connected to each other, at least a portion of the first connecting portion is accommodated in the bag body, a notch is formed at an end of the first connecting portion located in the bag body, and the second connecting portion is fixed to a surface of the first film layer facing away from the second film layer; and The mounting hole penetrates the bag body along the thickness direction, and the mounting hole is configured to mount the centrifuge bag in a centrifugal device.

2. The centrifuge bag according to claim 1, characterized in that The centrifuge bag further comprises an anti-absorption sheet, which is arranged on the surface of the second film layer and corresponds to the connecting piece, and the hardness of the anti-absorption sheet is greater than the hardness of the second film layer.

3. The centrifuge bag according to claim 2, characterized in that The anti-absorption sheet is arranged on the surface of the second film layer facing away from the first film layer, or the anti-absorption sheet is arranged on the surface of the second film layer close to the first film layer.

4. The centrifuge bag according to claim 2, characterized in that The anti-absorption sheet includes an arc-shaped portion and an extension portion connected to the edge of the arc-shaped portion, the arc-shaped portion is recessed in a direction away from the notch, and the extension portion is arranged corresponding to the second connecting portion.

5. The centrifuge bag according to claim 1, characterized in that The edge of the mounting hole has a first sealing portion, and the first sealing portion is formed by sealing the first film layer and the second film layer located at the edge of the mounting hole.

6. The centrifuge bag according to claim 5, characterized in that The bag body has a second sealing portion, the second sealing portion is arranged around the first sealing portion, and the second sealing portion is formed by sealing parts of the first film layer and the second film layer.

7. The centrifuge bag according to claim 1, characterized in that The centrifuge bag further includes a sample tube, which is connected to an end of the first connection portion away from the notch and is communicated with the interior of the bag body through the first connection portion.

8. The centrifuge bag according to claim 1, characterized in that The bag body comprises a first area and a second area which are connected to each other. The second area is connected to the edge of the first area. The connecting piece and the mounting hole are arranged in the first area. The second area is used for accommodating the sample after centrifugation.

9. The centrifuge bag according to claim 1, characterized in that There are a plurality of mounting holes, and the plurality of mounting holes are arranged around the connecting member with the connecting member as the center.

10. The centrifuge bag according to claim 1, characterized in that The material of the bag body and the connecting piece is independently one or more of polyvinyl chloride, polyethylene, ethylene-vinyl acetate copolymer and thermoplastic polyurethane.

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