A chromatographic column tube structure provided with a flow guiding structure

Abstract

The present application discloses a chromatographic column tube structure provided with a flow guiding structure, specifically relating to the technical field of chromatographic columns. It includes a column tube main body, a first flow guiding assembly, and a second flow guiding assembly. The water inlet end of the column tube main body is connected to the first flow guiding assembly, and the water outlet end of the column tube main body is connected to the second flow guiding assembly. The column tube main body includes a tube wall, a spiral flow guiding groove, and a micro turbulence generating fin. The inner wall of the tube wall is provided with the spiral flow guiding groove, and the micro turbulence generating fin is arranged on the side of the tube wall away from the first flow guiding assembly. Therefore, when the sample flows along the spiral flow guiding groove in the column tube main body, the spiral flow guiding groove will force the sample to flow along a spiral path, enhancing radial mixing, reducing bypass flow and dead volume. At the same time, the arranged micro turbulence generating fin generates local turbulence for the sample in the column tube main body after flowing, destroying the laminar boundary layer and improving the mass transfer efficiency.

Description

Technical Field

[0001] The present application relates to the technical field of chromatographic columns, and more specifically, to a chromatographic column tube structure provided with a flow guiding structure. Background Art

[0002] The chromatographic column is a key component used in chromatographic analysis. It separates the components in a mixture by using the difference in the distribution coefficients of different substances between the stationary phase and the mobile phase. In recent years, with the continuous advancement of materials science and analytical technology, the performance and application range of chromatographic columns have been further improved, making the chromatographic columns more capable of separating complex samples. At the same time, the miniaturization and high-throughput technology of chromatographic columns have also developed rapidly, making chromatographic analysis more efficient and faster. However, problems such as sample bias or dead volume often occur in the chromatographic column.

[0003] After searching, the existing publication number: CN109891232A, discloses a column for chromatography; the filling material can be filled in the most suitable compression state, thereby preventing the mobile phase containing the test body from leaking out of the tube. The column mainly includes: a cylindrical tube, a tube plug, a first filter, a second filter, a plug, and a connector. The tube mainly includes: a cylindrical storage part with a bottom, and a cylindrical bottom side connecting part connected to the bottom of the storage part. The storage part and the bottom side connecting part are arranged on the same axis. The tube plug mainly includes: a cylindrical outer cylinder part arranged on the same axis, and an inner cylinder part arranged on the inner side of the outer cylinder part. The first filter and the second filter are filters of a degree that the filling material cannot pass through. The plug mainly includes a plug holding part and a plug insertion part. The connector mainly includes a connector holding part and a connector insertion part. In the process of realizing this application, the inventor found that the prior art has the following problems:

[0004] When existing spectral columns are used, bias flow and dead volume often occur. Bias flow can cause uneven distribution of samples in the column, poor separation effect, broadening or tailing of peaks, and dead volume can cause samples to be retained in certain areas of the column and unable to effectively participate in separation, resulting in prolonged analysis time or unsatisfactory peak shape.

[0005] Therefore, in order to solve the above problems, a chromatographic column tube structure with a flow guide structure is proposed. Summary of the invention

[0006] In order to overcome the above-mentioned defects of the prior art, the present application provides a chromatography column tube structure provided with a flow guide structure to solve the problems raised in the above-mentioned background technology.

[0007] To achieve the above object, the present application provides the following technical solution: A chromatographic column tube structure provided with a flow guiding structure, comprising a column tube main body, a first flow guiding component and a second flow guiding component. The water inlet end of the column tube main body is connected with the first flow guiding component, and the water outlet end of the column tube main body is connected with the second flow guiding component. The column tube main body includes a tube wall, a spiral flow guiding groove and a micro turbulator. A spiral flow guiding groove for forcing the sample to advance along a spiral path is provided on the inner wall of the tube wall, and a micro turbulator for generating local turbulence in the flow of the sample and destroying the laminar boundary is provided on the side of the tube wall away from the first flow guiding component; and tiny protrusions are provided on the outer surface of the micro turbulator.

[0008] Preferably, the column tube main body includes a temperature control element, a micro pressure sensor, an optical window and a delivery cavity. A temperature control element is installed on the outer diameter surface of the column tube main body. A micro pressure sensor is provided below the temperature control element. An optical window is provided below the micro pressure sensor. A delivery cavity is provided on the side of the spiral flow guiding groove away from the temperature control element.

[0009] Preferably, the column tube main body further includes a first pre-installation interface, a second pre-installation interface and an external thread wall. A first pre-installation interface is installed at the top of the column tube main body. A second pre-installation interface is provided on the side of the column tube main body away from the first pre-installation interface. An external thread wall is provided at one end of the second pre-installation interface away from the first pre-installation interface.

[0010] Preferably, the first flow guiding component includes a fixed collar, an adsorption ring, a pressurizing tube, a first mounting ring and a connecting tube. An adsorption ring is installed at one end of the fixed collar away from the column tube main body. A pressurizing tube is installed at one end of the adsorption ring away from the fixed collar. A first mounting ring is installed at one end of the pressurizing tube away from the adsorption tube. A connecting tube is installed at one end of the first mounting ring away from the pressurizing tube.

[0011] Preferably, the second flow guiding component includes a locking ring, an internal thread wall and a locking shaft. An internal thread wall is provided at the edge of the inner wall of the locking ring. A locking shaft is connected to the side of the locking ring.

[0012] Preferably, the second flow guiding component further includes an external connecting tube, a mounting tube and an insertion tube. A mounting tube is installed at one end of the external connecting tube away from the locking ring. An insertion tube is installed at one end of the mounting tube away from the external connecting tube.

[0013] Preferably, plug-in injection cores are inserted at both ends of the inner wall of the column tube body. The plug-in injection core includes a sealing ring, a liquid inlet, a fixing ring, a liquid inlet pipe, a second mounting ring, and a fixing pipe. A sealing ring is placed between the column tube body and the plug-in injection core. A liquid inlet is provided through the center of the sealing ring. A liquid inlet pipe is placed on the side of the liquid inlet away from the column tube body. A fixing ring is sleeved on the outer diameter surface of the liquid inlet pipe. A second mounting ring is sleeved on the outer diameter surface of the fixing ring. A fixing pipe is provided on the side of the second mounting ring away from the sealing ring.

[0014] Preferably, an outlet is provided at one end of the column tube body away from the liquid inlet. An outlet pipe is placed at the end of the outlet away from the liquid inlet. A third mounting ring is sleeved on the outer diameter surface of the outlet pipe.

[0015] Preferably, a docking ring is installed between the column tube body and the first flow guiding component. A detachable structure is formed between the column tube body and the first flow guiding component through the docking ring. A detachable structure is formed between the column tube body and the second flow guiding component through an outer threaded wall and an inner threaded wall.

[0016] Technical effects and advantages of this application:

[0017] 1. Compared with the prior art, this chromatographic column tube structure with a flow guiding structure is composed of a column tube body, a first flow guiding component, and a second flow guiding component. When using the column tube body structure, when a sample enters the inside of the column tube body, the spiral flow guiding grooves provided on the inner wall of the column tube body force the sample to move along a spiral path, guiding the sample and the mobile phase to form a more uniform and stable flow pattern in the column, thereby improving the separation performance. Moreover, micro turbulators are also provided in the tube wall to adjust the flow properties of the sample in the column tube body. Therefore, small protrusions are provided in the column to generate local turbulence in the flow of the sample, destroying the laminar boundary layer and avoiding the mass transfer resistance caused by the "wall attachment flow" of the mobile phase, making the flow domain of the sample in the column tube body change from laminar flow to more nearly plug flow, reducing axial diffusion. Thus, when the sample fluid enters the inside of the column tube body, it can guide the sample fluid to flow forward evenly, reducing turbulent flow and stagnant areas.

[0018] 2. Compared with the prior art, for this chromatographic column tube structure with a flow guiding structure, when the sample flows along the spiral flow guiding grooves in the column tube body, the spiral flow guiding grooves force the mobile phase to flow along a spiral path, enhancing radial mixing, reducing bypass flow and dead volume. At the same time, the micro turbulators provided generate local turbulence in the sample in the column tube body after flowing, destroying the laminar boundary layer and improving the transmission efficiency. Description of the Drawings

[0019] Figure 1Schematic diagram of the overall structure of the present application;

[0020] Figure 2 Schematic diagram of the front view cross-section structure of the column tube main body of the present application;

[0021] Figure 3 Schematic diagram of the front view cross-section structure of the micro spoiler of the present application;

[0022] Figure 4 Schematic diagram of the front view cross-section structure of the plug-in injection core of the present application;

[0023] Figure 5 For the present application Figure 3 Schematic diagram of the structure at position A of the present application;

[0024] Figure 6 For the present application Figure 4 Schematic diagram of the structure at position B of the present application;

[0025] Figure 7 Schematic diagram of the docking ring of the present application;

[0026] Figure 8 Schematic diagram of the pipe wall of the present application;

[0027] Figure 9 Schematic diagram of the locking ring of the present application.

[0028] Reference numerals in the drawings are: 1, column tube main body; 101, pipe wall; 102, spiral flow guiding groove; 103, temperature control element; 104, micro pressure sensor; 105, optical window; 106, conveying cavity; 107, micro spoiler; 108, first pre-installation interface; 109, second pre-installation interface; 1010, external thread wall; 2, first flow guiding assembly; 201, fixing collar; 202, adsorption ring; 203, pressurizing pipe; 204, first installation ring; 205, connecting pipe; 3, second flow guiding assembly; 301, locking ring; 302, internal thread wall; 303, locking shaft; 304, external connecting pipe; 305, installation pipe; 306, inserting pipe; 4, plug-in injection core; 5, sealing ring; 6, liquid inlet; 7, fixing ring; 8, liquid inlet pipe; 9, second installation ring; 10, fixing pipe; 11, liquid outlet; 12, liquid outlet pipe; 13, third installation ring; 14, docking ring. Detailed implementation manners

[0029] Next, the technical solutions in the embodiments of the present application will be clearly and completely described in conjunction with the drawings in the embodiments of the present application. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present application. Embodiment

[0030] As shown in the attached Figures 1 to 9 chromatographic column tube structure with a diversion structure, including a column tube main body 1, a first diversion component 2 and a second diversion component 3. The water inlet end of the column tube main body 1 is connected to the first diversion component 2, and the water outlet end of the column tube main body 1 is connected to the second diversion component 3. The column tube main body 1 includes a tube wall 101, a spiral diversion groove 102 and a micro turbulator 107. A spiral diversion groove 102 for forcing the sample to advance along a spiral path is provided on the inner wall of the tube wall 101, and a micro turbulator 107 for generating local turbulence in the flow of the sample and destroying the laminar boundary is provided on the side of the tube wall 101 away from the first diversion component 2; and there are small protrusions on the outer surface of the micro turbulator 107.

[0031] Among them, the chromatographic column tube structure is composed of a column tube main body 1, a first diversion component 2 and a second diversion component 3. When using the structure of the column tube main body 1, when the sample enters the inside of the column tube main body 1, at this time, the spiral diversion groove 102 provided on the tube wall 101 of the inner wall of the column tube main body 1 forces the mobile phase to advance along a spiral path, guiding the sample and the mobile phase to form a more uniform and stable flow pattern in the column, thereby improving the separation performance. And a micro turbulator 107 is also provided in the tube wall 101 to adjust the flow properties of the sample in the column tube main body 1. Therefore, the small protrusions provided in the column generate local turbulence in the flow of the sample, destroy the laminar boundary layer, avoid the mass transfer resistance caused by the "wall attachment flow" of the mobile phase, make the flow domain of the sample flowing in the column tube main body 1 change from laminar flow to a more plug flow, reduce axial diffusion, and also reduce the theoretical plate height, showing a narrower and more symmetrical chromatographic peak, improving the resolution, and reducing the performance fluctuations caused by column packing differences or long-term use, prolonging the life of the chromatographic column. Imagining the chromatographic column as a river, when there is no diversion structure inside the column tube main body 1, it will cause the water flow to form vortices or tributaries (lateral flow) due to the uneven river bed, and the water flow will stagnate in some areas (dead volume). When there is a diversion structure inside the column tube main body 1, such as setting a diversion plate or an artificial channel in the river bed to guide the water flow to flow forward evenly, reducing the turbulent flow and retention areas. Therefore, when the sample flows along the spiral diversion groove 102 in the column tube main body 1, the spiral diversion groove 102 will force the mobile phase to flow along a spiral path, enhancing the radial mixing, reducing the lateral flow and dead volume. At the same time, the micro turbulator 107 provided generates local turbulence in the sample flowing in the column tube main body 1, destroys the laminar boundary layer, and improves the mass transfer efficiency. Embodiment

[0032] Based on Embodiment 1, the solution in Embodiment 1 will be further refined and introduced in combination with the following specific working methods, as Figures 1 to 9 shown, see the following description for details:

[0033] As a preferred embodiment, the column tube body 1 includes a temperature control element 103, a micro pressure sensor 104, an optical window 105, and a delivery chamber 106. The temperature control element 103 is installed on the outer diameter surface of the column tube body 1. The micro pressure sensor 104 is disposed below the temperature control element 103. The optical window 105 is disposed below the micro pressure sensor 104. The delivery chamber 106 is disposed on the side of the spiral flow guiding groove 102 away from the temperature control element 103. As a preferred embodiment, the column tube body 1 includes a temperature control element 103, a micro pressure sensor 104, an optical window 105, and a delivery chamber 106. The temperature control element 103 is installed on the outer diameter surface of the column tube body 1. The micro pressure sensor 104 is disposed below the temperature control element 103. The optical window 105 is disposed below the micro pressure sensor 104. The delivery chamber 106 is disposed on the side of the spiral flow guiding groove 102 away from the temperature control element 103. The model of the temperature control element 103 is Custom-Thermoelectric-12711-5L31-03CL. The temperature control element 103 precisely controls the column temperature by integrating a resistance wire on the outer wall of the column tube body 1 to optimize the separation selectivity. The model of the micro pressure sensor 104 is XJC-Z15-H9-2M. The micro pressure sensor 104 monitors the pressure of the first-dimensional chromatographic column of the column tube body 1, triggers fraction collection, and real-time feedbacks the pressure change to adjust the backflush flow rate. Then, the optical window 105 provided in the column tube body 1 allows on-line detection to perform ultraviolet absorption and fluorescence detection on part of the sample. The optical window 105 is made of quartz or sapphire transparent material. The delivery chamber 106 is the space in the column tube body 1 for delivering the sample, enabling the sample to pass through the delivery chamber 106 and guiding the flow.

[0034] As a preferred embodiment, the column tube body 1 further includes a first pre-installed interface 108, a second pre-installed interface 109, and an external thread wall 1010. The first pre-installed interface 108 is installed at the top of the column tube body 1. The second pre-installed interface 109 is disposed on the side of the column tube body 1 away from the first pre-installed interface 108. The external thread wall 1010 is disposed at one end of the second pre-installed interface 109 away from the first pre-installed interface 108. The first pre-installed interface 108 and the second pre-installed interface 109 are installed at the ports at both ends of the column tube body 1. The structures of the first pre-installed interface 108 and the second pre-installed interface 109 are the same. The first pre-installed interface 108 provided at the water inlet end of the column tube body 1 is used to connect to the docking ring 14, enabling the column tube body 1 to be quickly connected to the docking ring 14 through the first pre-installed interface 108 to reduce the risk of dead volume and leakage.

[0035] As a preferred embodiment, the first flow guiding assembly 2 includes a fixed collar 201, an adsorption ring 202, a pressure pipe 203, a first mounting ring 204 and a connecting pipe 205. One end of the fixed collar 201 away from the column pipe body 1 is provided with the adsorption ring 202, one end of the adsorption ring 202 away from the fixed collar 201 is provided with the pressure pipe 203, one end of the pressure pipe 203 away from the adsorption pipe is provided with the first mounting ring 204, and one end of the first mounting ring 204 away from the pressure pipe 203 is provided with the connecting pipe 205. The fixed collar 201 is the starting end of the first flow guiding assembly 2 as a component and is directly connected to the first pre-installation interface 108 of the column pipe body 1. When the first flow guiding assembly 2 is connected to the column pipe body 1, while providing mechanical fixation for the column pipe body 1, it is used to ensure the coaxiality between the component and the column pipe. The first flow guiding assembly 2 and the column pipe body 1 are quickly disassembled and assembled through threads. When the sample enters the column pipe body 1 through the first flow guiding assembly 2, the sample passes through the inside of the adsorption ring 202. When the sample passes through the adsorption ring 202, the sample will come into contact with the activated carbon in the adsorption ring 202 to capture trace impurities in the sample. And the adsorption ring 202 has a detachable design structure for facilitating the regular replacement of the adsorption ring 202. Before the sample enters the adsorption ring 202, it will enter the inside of the pressure pipe 203. The inner diameter of the pressure pipe 203 is a gradient structure for adjusting the pressure of the sample during the process of passing through the adsorption ring 202. And the pressure pipe 203 is linked with the external gas path system, and the flow rate is controlled by the pressure feedback set by the box body connecting the column pipe body 1 to control the flow mode of the sample. The first mounting ring 204 is used for the mechanical connection between the connecting pipe 205 and the pressure pipe 203. The first mounting ring 204 provides a standardized interface for adapting to external devices for connection. The connecting pipe 205 is used as the port connection interface of the first flow guiding assembly 2. The inner wall of the connecting pipe 205 is polished to reduce the dead volume of the sample flow path in the first flow guiding assembly 2. The sample flows out from the connecting pipe 205 → through the pressure pipe 203 connected by the first mounting ring 204 → the sample after pressure adjustment → the sample after impurities are adsorbed enters the fixed collar 201 → and finally is transported by the fixed collar 201 to the downstream column pipe body 1. The first flow guiding assembly 2 adopts metal sealing and has a leakage rate of ≤ 1×10⁻ 9 Pa·m³ / s.

[0036] As a preferred embodiment, the second diversion assembly 3 includes a locking ring 301, an internal thread wall 302 and a locking shaft 303. The internal thread wall 302 is provided at the inner wall edge of the locking ring 301, and the locking shaft 303 is connected to the side of the locking ring 301. The locking ring 301 is located at the inner wall edge of the locking ring 301, where the thread specification is M6×0.75, which is used to quickly tighten with the external thread of the column tube. And the locking ring 301 is directly embedded in the side wall of the locking ring 301. By adopting an eccentric cam structure, an axial pre-tightening force of ≥50 N·m can be generated during tightening to prevent loosening under high-pressure conditions.

[0037] As a preferred embodiment, the second diversion assembly 3 further includes an external connection pipe 304, a mounting pipe 305 and an insertion connection pipe 306. One end of the external connection pipe 304 away from the locking ring 301 is provided with the mounting pipe 305, and one end of the mounting pipe 305 away from the external connection pipe 304 is provided with the insertion connection pipe 306. When the sample enters the inside of the second diversion assembly 3 through the column tube body 1, at this time, the sample enters the cavity of the external connection pipe 304. The external connection pipe 304 is the transition flow path of the second diversion assembly 3. The inner diameter of the external connection pipe 304 matches that of the column tube, with a tolerance of ±0.01 mm to avoid sudden change in flow rate. And the connection between the mounting pipe 305 and the insertion connection pipe 306 is used to connect with the equipment.

[0038] As a preferred embodiment, insertion injection cores 4 are inserted at both ends of the inner wall of the column tube body 1. The insertion injection core 4 includes a sealing ring 5, a liquid inlet 6, a fixing ring 7, a liquid inlet pipe 8, a second mounting ring 9 and a fixing pipe 10. The sealing ring 5 is placed between the column tube body 1 and the insertion injection core 4. The liquid inlet 6 is penetrated through the center of the sealing ring 5. The liquid inlet pipe 8 is placed on the side of the liquid inlet 6 away from the column tube body 1. The fixing ring 7 is sleeved on the outer diameter surface of the liquid inlet pipe 8. The second mounting ring 9 is sleeved on the outer diameter surface of the fixing ring 7. The fixing pipe 10 is provided on the side of the second mounting ring 9 away from the sealing ring 5. The material of the sealing ring 5 is perfluoroether rubber. The liquid inlet pipe 8 is the flow structure for the first diversion assembly 2 to enter the column tube body 1. The material of the liquid inlet pipe 8 is titanium alloy, which has corrosion resistance and biocompatibility. And the inner diameter of the liquid inlet of the liquid inlet pipe 8 is tapered from 1.2 mm to 0.5 mm to achieve laminar flow transition. Then the fixing ring 7 is used to position the liquid inlet pipe 8. By interference fit, the axial position of the liquid inlet pipe 8 is fixed. And through the annular groove design, the stress concentration of the pipe wall 101 under high pressure is reduced. Then the second mounting ring 9 is sleeved on the outer surface of the liquid inlet pipe 8. Gold is plated on the outer surface of the second mounting ring 9 to prevent static electricity accumulation. And the fixing ring 7 is used to fix the liquid inlet pipe 8 at the final end. The fixing ring 7 is connected to the external equipment through a clamp structure.

[0039] As a preferred embodiment, an outlet 11 is provided at one end of the column tube body 1 away from the liquid inlet 6, and an outlet tube 12 is placed at one end of the outlet 11 away from the liquid inlet 6. A third mounting ring 13 is sleeved on the outer diameter surface of the outlet tube 12, and the structures of the liquid inlet end and the liquid outlet end of the column tube body 1 are the same.

[0040] As a preferred embodiment, a docking ring 14 is installed between the column tube body 1 and the first flow guiding assembly 2. The column tube body 1 and the first flow guiding assembly 2 form a detachable structure through the docking ring 14, and the column tube body 1 and the second flow guiding assembly 3 form a detachable structure through the external thread wall 1010 and the internal thread wall 302.

[0041] The working process of this application is as follows: First, the chromatographic column tube structure is composed of a column tube body 1, a first flow guiding assembly 2, and a second flow guiding assembly 3. When using the structure of the column tube body 1, when the sample enters the inside of the column tube body 1, at this time, the spiral flow guiding groove 102 provided on the tube wall 101 of the inner wall of the column tube body 1 forces the mobile phase to advance along a spiral path, guiding the sample and the mobile phase to form a more uniform and stable flow pattern in the column, thereby improving the separation performance. Moreover, micro turbulators 107 are also provided in the tube wall 101 to adjust the flow properties of the sample in the column tube body 1. Therefore, the tiny protrusions provided in the column generate local turbulence in the flow of the sample, destroying the laminar boundary layer, avoiding the mass transfer resistance caused by the "wall attachment flow" of the mobile phase, making the flow domain of the sample in the column tube body 1 change from laminar flow to a more plug-like flow, reducing axial diffusion, and also reducing the theoretical plate height, showing a narrower and more symmetrical chromatographic peak, improving the resolution, and reducing the performance fluctuations caused by column packing differences or long-term use, prolonging the life of the chromatographic column. Imagining the chromatographic column as a river, when there is no flow guiding structure inside the column tube body 1, it may cause vortices or tributaries (biased flow) due to the uneven river bed, and the water flow stagnates in some areas (dead volume). When there is a flow guiding structure inside the column tube body 1, such as setting flow guiding plates or artificial channels in the river bed to guide the water flow to flow forward evenly, reducing turbulent flow and stagnant areas. Therefore, when the sample flows along the spiral flow guiding groove 102 in the column tube body 1, the spiral flow guiding groove 102 forces the mobile phase to flow along a spiral path, enhancing radial mixing, reducing biased flow and dead volume. At the same time, the micro turbulators 107 provided generate local turbulence in the sample in the column tube body 1 after flowing, destroying the laminar boundary layer, and improving the mass transfer efficiency.

[0042] Among them, the temperature control element 103 precisely controls the column temperature by integrating a resistance wire on the outer wall of the column tube body 1, optimizing the separation selectivity. The model of the temperature control element 103 is Custom-Thermoelectric-12711-5L31-03CL, and the model of the micro pressure sensor 104 is XJC-Z15-H9-2M. The micro pressure sensor 104 monitors the pressure of the first-dimensional chromatographic column in the column tube body 1, triggers fraction collection, and real-time feedback of pressure changes to adjust the backflush flow rate. Then, the optical window 105 provided in the column tube body 1 allows on-line detection for ultraviolet absorption and fluorescence detection of part of the sample. The optical window 105 is made of quartz or sapphire transparent material, and the delivery cavity 106 is the space in the column tube body 1 for delivering the sample, enabling the sample to pass through the delivery cavity 106 and guiding the flow.

[0043] At both ends of the column tube body 1, a first pre-installed interface 108 and a second prefabricated structure are installed. The structures of the first pre-installed interface 108 and the second prefabricated structure are the same. The first pre-installed interface 108 provided at the water inlet end of the column tube body 1 is used to connect to the docking ring 14, enabling the column tube body 1 to be quickly connected to the docking ring 14 through the first pre-installed interface 108, reducing the risk of dead volume and leakage.

[0044] Among them, the fixed collar 201 is the starting end of the first diversion component 2 as a component, and is directly connected to the first pre-installation interface 108 of the column tube body 1. After the first diversion component 2 is connected to the column tube body 1, while providing mechanical fixation for the column tube body 1, it is used to ensure the coaxiality between the component and the column tube. The first diversion component 2 and the column tube body 1 are quickly disassembled and assembled through threads. When the sample enters the column tube body 1 through the first diversion component 2, the sample passes through the inside of the adsorption ring 202. When the sample passes through the adsorption ring 202, the sample will come into contact with the activated carbon in the adsorption ring 202 to capture trace impurities in the sample. And the adsorption ring 202 has a detachable design structure for facilitating the regular replacement of the adsorption ring 202. Before the sample enters the adsorption ring 202, it will enter the inside of the pressure tube 203. The inner diameter of the pressure tube 203 is a gradually changing structure for adjusting the pressure of the sample during the process of passing through the adsorption ring 202. And the pressure tube 203 is linked with the external gas path system, and the flow rate is controlled by the pressure feedback set in the box body connecting the column tube body 1 to control the flow mode of the sample. The first mounting ring 204 is used for the mechanical connection between the connecting tube 205 and the pressure tube 203. The first mounting ring 204 provides a standardized interface for adapting to external devices for connection. The connecting tube 205 is used as the port connection interface of the first diversion component 2. The inner wall of the connecting tube 205 is polished to reduce the dead volume of the flow path of the sample in the first diversion component 2. The sample flows out from the connecting tube 205 → passes through the pressure tube 203 connected by the first mounting ring 204 → the sample after pressure adjustment → the impurities are adsorbed and then enter the fixed collar 201 → and finally is transported by the fixed collar 201 to the downstream column tube body 1. The first diversion component 2 adopts metal sealing and ≤1×10⁻ 9 Pa·m³ / s leakage rate.

[0045] The locking ring 301 is located at the inner wall edge of the locking ring 301, and the thread specification is M6×0.75., which is used for quickly screwing tightly with the external thread of the column tube. And the locking ring 301 is directly embedded in the side wall of the locking ring 301. By adopting an eccentric cam structure, an axial pre-tightening force of ≥50 N·m can be generated during screwing to prevent loosening under high-pressure conditions.

[0046] Then when the sample enters the inside of the second diversion component 3 through the column tube body 1, at this time the sample enters the cavity of the external connecting tube 304. The external connecting tube 304 is the transition flow path of the second diversion component 3. The inner diameter of the external connecting tube 304 matches that of the column tube, and the tolerance is ±0.01 mm to avoid sudden change of flow rate. And the connection between the mounting tube 305 and the insertion connecting tube 306 is used for connection with the equipment.

[0047] Among them, the material of the sealing ring 5 is perfluoroether rubber, and the liquid inlet pipe 8 is the flow structure for the first diversion assembly 2 to enter the column tube main body 1. The material of the liquid inlet pipe 8 is titanium alloy, which has corrosion resistance and biocompatibility. And the inner diameter of the liquid inlet of the liquid inlet pipe 8 gradually shrinks from 1.2 mm to 0.5 mm to achieve laminar flow transition. Then the fixing ring 7 is used to position the liquid inlet pipe 8, and the axial position of the liquid inlet pipe 8 is fixed through interference fit. And through the annular groove design, the stress concentration on the pipe wall 101 under high pressure is reduced. Then the second mounting ring 9 is sleeved on the outer surface of the liquid inlet pipe 8. Gold is plated on the outer surface of the second mounting ring 9 to prevent static electricity accumulation. And the fixing ring 7 is used to fix the end of the liquid inlet pipe 8. The fixing ring 7 is connected to external equipment through a clamp structure. The structures of the liquid inlet end and the liquid outlet end of the column tube main body 1 are the same. The above is the working principle of the column tube structure of a chromatographic column with a diversion structure.

Claims

1. A chromatographic column tube structure with a flow guiding structure, comprising a column tube main body (1), a first flow guiding component (2) and a second flow guiding component (3), characterized in that: The water inlet end of the column tube body (1) is connected with a first flow guiding component (2), and the water outlet end of the column tube body (1) is connected with a second flow guiding component (3). The column tube body (1) includes a tube wall (101), a spiral flow guiding groove (102) and a micro turbulator (107). A spiral flow guiding groove (102) for forcing the sample to advance along a spiral path is arranged on the inner wall of the tube wall (101). A micro turbulator (107) which generates local turbulence in the flow of the sample and destroys the laminar boundary is arranged on the side of the tube wall (101) away from the first flow guiding component (2), and tiny protrusions are arranged on the outer surface of the micro turbulator (107).

2. The column tube structure of a chromatographic column with a diversion structure according to claim 1, characterized in that: The column tube body (1) includes a temperature control element (103), a micro pressure sensor (104), an optical window (105) and a delivery cavity (106). A temperature control element (103) is installed on the outer diameter surface of the column tube body (1). A micro pressure sensor (104) is arranged below the temperature control element (103). An optical window (105) is arranged below the micro pressure sensor (104). A delivery cavity (106) is arranged on the side of the spiral flow guiding groove (102) away from the temperature control element (103).

3. The column tube structure of a chromatographic column with a diversion structure according to claim 1, characterized in that: The column tube body (1) further includes a first pre-installation interface (108), a second pre-installation interface (109), and an external thread wall (1010). A first prefabricated interface is installed at the top of the column tube body (1). A second pre-installation interface (109) is arranged on the side of the column tube body (1) away from the first pre-installation interface (108). An external thread wall (1010) is arranged at one end of the second pre-installation interface (109) away from the first prefabricated interface.

4. A chromatographic column tube structure with a flow guiding structure according to claim 1, characterized in that: The first flow guiding component (2) includes a fixed collar (201), an adsorption ring (202), a pressure pipe (203), a first installation ring (204) and a connecting pipe (205). An adsorption ring (202) is installed at one end of the fixed collar (201) away from the column tube body (1). A pressure pipe (203) is installed at one end of the adsorption ring (202) away from the fixed collar (201). A first installation ring (204) is installed at one end of the pressure pipe (203) away from the adsorption pipe. A connecting pipe (205) is installed at one end of the first installation ring (204) away from the pressure pipe (203).

5. A chromatographic column tube structure with a diversion structure according to claim 1, characterized in that: The second flow guiding component (3) includes a locking ring (301), an internal thread wall (302) and a locking shaft (303). An internal thread wall (302) is arranged at the edge of the inner wall of the locking ring (301). A locking shaft (303) is connected to the side of the locking ring (301).

6. The column tube structure of a chromatographic column with a flow guiding structure according to claim 1, characterized in that: The second flow guiding component (3) further includes an external connecting pipe (304), an installation pipe (305) and an insertion connecting pipe (306). An installation pipe (305) is installed at one end of the external connecting pipe (304) away from the locking ring (301). An insertion connecting pipe (306) is installed at one end of the installation pipe (305) away from the external connecting pipe (304).

7. A chromatographic column tube structure provided with a flow guiding structure according to claim 1, characterized in that: The inner walls at both ends of the column tube main body (1) are inserted with plug-in injection cores (4), and the plug-in injection cores (4) include sealing rings (5), liquid inlets (6), fixing rings (7), liquid inlet pipes (8), second mounting rings (9) and fixing pipes (10). A sealing ring (5) is placed between the column tube main body (1) and the plug-in injection core (4). A liquid inlet (6) is penetrated through the center of the sealing ring (5). A liquid inlet pipe (8) is placed on the side of the liquid inlet (6) away from the column tube main body (1). A fixing ring (7) is sleeved on the outer diameter surface of the liquid inlet pipe (8). A second mounting ring (9) is sleeved on the outer diameter surface of the fixing ring (7). A fixing pipe (10) is arranged on the side of the second mounting ring (9) away from the sealing ring (5).

8. A chromatographic column tube structure with a diversion structure according to claim 1, characterized in that: One end of the column tube main body (1) away from the liquid inlet (6) is provided with a liquid outlet (11). A liquid outlet pipe (12) is placed on the end of the liquid outlet (11) away from the liquid inlet (6). A third mounting ring (13) is sleeved on the outer diameter surface of the liquid outlet pipe (12).

9. A column tube structure of a chromatographic column provided with a flow guiding structure according to claim 1, characterized in that: A docking ring (14) is installed between the column tube main body (1) and the first flow guiding component (2). The column tube main body (1) and the first flow guiding component (2) form a detachable structure through the docking ring (14). The column tube main body (1) and the second flow guiding component (3) form a detachable structure through an external thread wall (1010) and an internal thread wall (302).

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