377 whitening and antioxidant composition and application thereof in cosmetics

Abstract

The invention belongs to the technical field of cosmetics, and particularly relates to a 377 whitening and antioxidant composition and application thereof in cosmetics. In the composition provided by the invention, the components such as 377, 577, arbutin, nicotinamide and the like have a whitening effect, and the components such as vitamin E, coenzyme Q10 and the like have an anti-oxidation effect; the lactic acid bacteria fermentation product and the morchella esculenta extract have whitening and anti-oxidation effects, and after the lactic acid bacteria fermentation product and the morchella esculenta extract are combined, the anti-oxidation effect can be multiplied. Other auxiliary materials such as glycerol and butanediol are used for moisturizing, hydrolyzed collagen is used for repairing, and ethylhexylglycerol and phenoxyethanol are used as preservatives, so that the stability of the product is ensured. The components act together, so that formation of melanin can be inhibited in multiple paths, and good whitening and anti-oxidation effects are achieved.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetics, and specifically relates to a 377 whitening and antioxidant composition and application thereof in cosmetics. Background Art

[0002] As people pay more and more attention to skin health, whitening and anti-oxidation have become hot research directions in the field of cosmetics. Skin whitening and anti-oxidation can not only improve skin color, but also delay skin aging and reduce the formation of wrinkles and spots. Phenylethyl Resorcinol (377) is a highly effective whitening ingredient and has been widely used in cosmetics. 377 can achieve whitening effects by inhibiting the activity of tyrosinase and reducing the production of melanin. In addition, 377 also has good antioxidant properties, which can neutralize free radicals and protect the skin from oxidative damage.

[0003] However, a single whitening ingredient often fails to meet consumers' demand for comprehensive skin care. Therefore, researchers began to explore the combination of 377 with other whitening and antioxidant ingredients to enhance its efficacy. For example, Chinese invention patent CN11823385B discloses a composition with anti-aging and whitening effects, which includes a variety of natural plant extracts, such as verbascoside, isovalascoside, forsythiaside, etc. These ingredients have whitening and anti-radiation effects.

[0004] In terms of preparation methods, existing whitening and antioxidant compositions mostly use a simple mixing process, but this method often fails to fully exert the synergistic effect of the various components. Therefore, developing a composition that can effectively compound 377 with other whitening and antioxidant components and its preparation method has important practical application value. Summary of the invention

[0005] The purpose of the present invention is to provide a whitening and antioxidant composition, which can significantly improve the whitening effect while also exerting a higher antioxidant effect, effectively resisting the damage of free radicals to the skin, delaying skin aging, and improving the skin condition more lastingly and deeply.

[0006] The invention provides a whitening and antioxidant composition, comprising the following components: 0.5wt%-2wt% of 377, 0.1wt%-1wt% of 577, 0.2wt%-7wt% of arbutin, 2wt%-10wt% of niacinamide, 1wt%-5wt% of lactic acid bacteria fermentation product, 0.1wt%-2wt% of morel extract, 0.1wt%-1wt% of vitamin E, 0.01wt%-0.1wt% of coenzyme Q10, 5wt%-15wt% of glycerol, 3wt%-8wt% of butylene glycol, 0.05wt%-0.3wt% of sodium hyaluronate, 0.1wt%-1wt% of polyglutamic acid, 0.5wt%-3wt% of betaine, 1wt%-5wt% of hydrolyzed collagen, 2wt%-8wt% of squalane, 0.2wt%-1wt% of ethylhexylglycerol, 0.3wt%-1wt% of phenoxyethanol and the balance of water.

[0007] Preferably, the composition comprises the following components: 1wt%~2wt% of 377, 0.5wt%~1wt% of 577, 3wt%~5wt% of arbutin, 5wt%~8wt% of niacinamide, 2wt%~4wt% of lactic acid bacteria fermentation product, 0.5wt%~1.5wt% of morel extract, 0.2wt%~0.5wt% of vitamin E, 0.02wt%~0.05wt% of coenzyme Q10, 8wt%~12wt% of glycerol, 5wt%~7wt% of butylene glycol, 0.1wt%~0.2wt% of sodium hyaluronate, 0.3wt%~0.5wt% of polyglutamic acid, 1wt%~2wt% of betaine, 2wt%~4wt% of hydrolyzed collagen, 4wt%~6wt% of squalane, 0.5wt%~0.8wt% of ethylhexylglycerin, 0.5wt%~0.8wt% of phenoxyethanol and the balance of water.

[0008] Preferably, the arbutin is α-arbutin and / or β-arbutin.

[0009] Preferably, the added amount of the α-arbutin is 0.2wt%~2wt%, and the added amount of the β-arbutin is 1wt%~7wt%.

[0010] The present invention also provides the use of the composition described in the above technical solution in the preparation of whitening and antioxidant products.

[0011] The present invention also provides a whitening and antioxidant product, which comprises the composition described in the above technical solution.

[0012] The present invention also provides a method for preparing the product described in the above technical solution, comprising the following steps: S1. Add 377, 577, arbutin, niacinamide, lactic acid bacteria fermentation product, morel extract, vitamin E and coenzyme Q10 to butanediol and stir until completely dissolved; then add glycerin, sodium hyaluronate, polyglutamic acid, betaine and hydrolyzed collagen, mix well and add to the remaining water, stir well, centrifuge to remove bubbles, and obtain a viscous liquid; S2, injecting the viscous liquid into the microneedle mold by vacuuming to ensure that the liquid is evenly distributed; after vacuuming, recovering the excess viscous liquid on the surface of the mold to obtain a soluble microcrystalline needle tip; S3, take 2-10 parts of chitosan and 4-20 parts of hyaluronic acid solution, stir evenly, and centrifuge to remove bubbles to obtain a base solution; S4, adding the base solution to the soluble microcrystal needle tip, ensuring that the base solution evenly covers the needle tip; S5. Dry for 12 to 24 hours at a constant temperature of 35°C to 40°C and a humidity of 15% to 25%. De-mold after drying to obtain soluble microcrystals with whitening and antioxidant functions.

[0013] Preferably, the stirring rate is 500 rpm / min, and the stirring time is 15 to 40 min.

[0014] Preferably, the step of removing bubbles by centrifugation is: centrifugation at 4000 rpm / min for 10 min at 4°C.

[0015] Preferably, the molecular weight of the chitosan is 240,000 Da, and the molecular weight of the sodium hyaluronate is 100,000 Da.

[0016] The present invention has no strict requirement on the amount of the base solution used, and the amount used can be such that the base solution evenly covers the needle tip.

[0017] Beneficial effects of the present invention: Ingredients such as 377, 577 (4-Butylresorcinol, 577 for short), arbutin, and niacinamide have whitening effects, while ingredients such as vitamin E and coenzyme Q10 have antioxidant effects; lactic acid bacteria fermentation products and morel extracts have both whitening and antioxidant effects. When used together, their antioxidant effects can be multiplied. Other excipients such as glycerin and butylene glycol are used for moisturizing, hydrolyzed collagen is used for repair, and ethylhexylglycerin and phenoxyethanol are used as preservatives to ensure the stability of the product. These ingredients work together to inhibit the formation of melanin in multiple ways, and have excellent whitening and antioxidant effects.

[0018] The present invention utilizes microcrystal technology to embed the whitening and antioxidant composition in a soluble microcrystal needle body compounded with hyaluronic acid and chitosan, which helps the composition to break through the barrier effect and directly reach the dermis, thereby improving the efficiency of percutaneous absorption, while locking in skin moisture and further enhancing the whitening and antioxidant effects. DETAILED DESCRIPTION

[0019] In order to further illustrate the present invention, a 377 whitening and antioxidant composition provided by the present invention and its application in cosmetics are described in detail below in conjunction with examples, but they should not be construed as limiting the scope of protection of the present invention.

[0020] The substances used in the embodiments of the present invention are all registered ingredients in INCI, and the registration number information of each component is as follows: 377 (Phenylethyl Resorcinol), 577 (4-Butylresorcinol), Arbutin, Niacinamide, Lactobacillus Ferment Lysate, Morchella Extract, Tocopherol, Ubiquinone, Glycerin, Butylene Glycol, Sodium Hyaluronate, Polyglutamic Acid, Betaine, Hydrolyzed Collagen, Squalane, Ethylhexylglycerin, Phenoxyethanol, Chitosan, Hyaluronic Acid, Aqua.

[0021] Example 1 Weigh each component according to the following amounts: 377 1g, 577 0.5g, arbutin 3.5g, niacinamide 6g, lactic acid bacteria fermentation product 3g, morel extract 1g, vitamin E 0.5g, coenzyme Q10 0.05g, glycerin 10g, butylene glycol 5.5g, sodium hyaluronate 0.15g, polyglutamic acid 0.5g, betaine 1.5g, hydrolyzed collagen 3g, squalane 5g, ethylhexylglycerin 0.6g, phenoxyethanol 0.6g, water 57.6g.

[0022] Add 377, 577, arbutin, niacinamide, lactic acid bacteria fermentation product, morel extract, vitamin E and coenzyme Q10 to butanediol and stir until completely dissolved; then add glycerin, sodium hyaluronate, polyglutamic acid, betaine and hydrolyzed collagen, mix well and add to the remaining water, stir at 500 rpm / min for 20 min, centrifuge at 4°C and 4000 rpm / min for 10 min to remove bubbles, and obtain a viscous liquid; The viscous liquid is injected into the microneedle mold by vacuuming to ensure that the liquid is evenly distributed; after vacuuming, the excess viscous liquid on the surface of the mold is recovered to obtain a soluble microcrystalline needle tip; Take 10 parts of chitosan and 20 parts of hyaluronic acid solution, stir evenly, centrifuge at 4°C, 4000 rpm / min for 10 min to remove bubbles, and obtain a base solution; Adding the base solution to the soluble microcrystal needle tip, so that the base solution evenly covers the needle tip; Dry for 18 hours at a constant temperature of 35°C to 40°C and a humidity of 15% to 25%, and demold after drying to obtain soluble microcrystals with whitening and antioxidant functions.

[0023] Example 2 Weigh each component according to the following amounts: 377 1.5g, 577 0.75g, arbutin 4g, niacinamide 6.5g, lactic acid bacteria fermentation product 3g, morel extract 1g, vitamin E 0.35g, coenzyme Q10 0.03g, glycerin 10g, butylene glycol 6g, sodium hyaluronate 0.15g, polyglutamic acid 0.4g, betaine 1.5g, hydrolyzed collagen 3g, squalane 5g, ethylhexylglycerin 0.65g, phenoxyethanol 0.65g, water 55.52g.

[0024] Add 377, 577, arbutin, niacinamide, lactic acid bacteria fermentation product, morel extract, vitamin E and coenzyme Q10 to butanediol and stir until completely dissolved; then add glycerin, sodium hyaluronate, polyglutamic acid, betaine and hydrolyzed collagen, mix well and add to the remaining water, stir at 500 rpm / min for 20 min, centrifuge at 4°C and 4000 rpm / min for 10 min to remove bubbles, and obtain a viscous liquid; The viscous liquid is injected into the microneedle mold by vacuuming to ensure that the liquid is evenly distributed; after vacuuming, the excess viscous liquid on the surface of the mold is recovered to obtain a soluble microcrystalline needle tip; Take 10 parts of chitosan and 20 parts of hyaluronic acid solution, stir evenly, centrifuge at 4°C, 4000 rpm / min for 10 min to remove bubbles, and obtain a base solution; Adding the base solution to the soluble microcrystal needle tip, so that the base solution evenly covers the needle tip; Dry for 18 hours at a constant temperature of 35°C to 40°C and a humidity of 15% to 25%, and demold after drying to obtain soluble microcrystals with whitening and antioxidant functions.

[0025] Example 3 Weigh each component according to the following amounts: 377 1g, 577 0.5g, arbutin 3g, niacinamide 5g, lactic acid bacteria fermentation product 2g, morel extract 0.5g, vitamin E 0.2g, coenzyme Q10 0.02g, glycerin 8g, butylene glycol 5g, sodium hyaluronate 0.1g, polyglutamic acid 0.3g, betaine 1g, hydrolyzed collagen 2g, squalane 4g, ethylhexylglycerin 0.5g, phenoxyethanol 0.5g, water 66.38g.

[0026] Add 377, 577, arbutin, niacinamide, lactic acid bacteria fermentation product, morel extract, vitamin E and coenzyme Q10 to butanediol and stir until completely dissolved; then add glycerin, sodium hyaluronate, polyglutamic acid, betaine and hydrolyzed collagen, mix well and add to the remaining water, stir at 500 rpm / min for 20 min, centrifuge at 4°C and 4000 rpm / min for 10 min to remove bubbles, and obtain a viscous liquid; The viscous liquid is injected into the microneedle mold by vacuuming to ensure that the liquid is evenly distributed; after vacuuming, the excess viscous liquid on the surface of the mold is recovered to obtain a soluble microcrystalline needle tip; Take 10 parts of chitosan and 20 parts of hyaluronic acid solution, stir evenly, centrifuge at 4°C, 4000 rpm / min for 10 min to remove bubbles, and obtain a base solution; Adding the base solution to the soluble microcrystal needle tip, so that the base solution evenly covers the needle tip; Dry for 18 hours at a constant temperature of 35°C to 40°C and a humidity of 15% to 25%, and demold after drying to obtain soluble microcrystals with whitening and antioxidant functions.

[0027] Example 4 Weigh each component according to the following amounts: 377 2g, 577 1g, arbutin 5g, niacinamide 8g, lactic acid bacteria fermentation product 4g, morel extract 1.5g, vitamin E 0.5g, coenzyme Q10 0.05g, glycerin 12g, butylene glycol 7g, sodium hyaluronate 0.2g, polyglutamic acid 0.5g, betaine 2g, hydrolyzed collagen 4g, squalane 6g, ethylhexylglycerin 0.8g, phenoxyethanol 0.8g, water 44.65g.

[0028] Add 377, 577, arbutin, niacinamide, lactic acid bacteria fermentation product, morel extract, vitamin E and coenzyme Q10 to butanediol and stir until completely dissolved; then add glycerin, sodium hyaluronate, polyglutamic acid, betaine and hydrolyzed collagen, mix well and add to the remaining water, stir at 500 rpm / min for 20 min, centrifuge at 4°C and 4000 rpm / min for 10 min to remove bubbles, and obtain a viscous liquid; The viscous liquid is injected into the microneedle mold by vacuuming to ensure that the liquid is evenly distributed; after vacuuming, the excess viscous liquid on the surface of the mold is recovered to obtain a soluble microcrystalline needle tip; Take 10 parts of chitosan and 20 parts of hyaluronic acid solution, stir evenly, centrifuge at 4°C, 4000 rpm / min for 10 min to remove bubbles, and obtain a base solution; Adding the base solution to the soluble microcrystal needle tip, so that the base solution evenly covers the needle tip; Dry for 18 hours at a constant temperature of 35°C to 40°C and a humidity of 15% to 25%, and demold after drying to obtain soluble microcrystals with whitening and antioxidant functions.

[0029] Example 5 Weigh each component according to the following amounts: 377 1.2g, 577 0.6g, arbutin 3.5g, niacinamide 6g, lactic acid bacteria fermentation product 2.5g, morel extract 0.75g, vitamin E 0.3g, coenzyme Q10 0.03g, glycerin 9g, butylene glycol 5.5g, sodium hyaluronate 0.12g, polyglutamic acid 0.35g, betaine 1.25g, hydrolyzed collagen 2.5g, squalane 4.5g, ethylhexylglycerin 0.6g, phenoxyethanol 0.6g, water 60.7g.

[0030] Add 377, 577, arbutin, niacinamide, lactic acid bacteria fermentation product, morel extract, vitamin E and coenzyme Q10 to butanediol and stir until completely dissolved; then add glycerin, sodium hyaluronate, polyglutamic acid, betaine and hydrolyzed collagen, mix well and add to the remaining water, stir at 500 rpm / min for 20 min, centrifuge at 4°C and 4000 rpm / min for 10 min to remove bubbles, and obtain a viscous liquid; The viscous liquid is injected into the microneedle mold by vacuuming to ensure that the liquid is evenly distributed; after vacuuming, the excess viscous liquid on the surface of the mold is recovered to obtain a soluble microcrystalline needle tip; Take 10 parts of chitosan and 20 parts of hyaluronic acid solution, stir evenly, centrifuge at 4°C, 4000 rpm / min for 10 min to remove bubbles, and obtain a base solution; Adding the base solution to the soluble microcrystal needle tip, so that the base solution evenly covers the needle tip; Dry for 18 hours at a constant temperature of 35°C to 40°C and a humidity of 15% to 25%, and demold after drying to obtain soluble microcrystals with whitening and antioxidant functions.

[0031] Example 6 Weigh each component according to the following amount: 377 1.8g, 577 0.85g, arbutin 4.5g, niacinamide 7g, lactic acid bacteria fermentation product 3.5g, morel extract 1.25g, vitamin E 0.4g, coenzyme Q10 0.04g, glycerin 11g, butylene glycol 6.5g, sodium hyaluronate 0.17g, polyglutamic acid 0.45g, betaine 1.75g, hydrolyzed collagen 3.5g, squalane 5.5g, ethylhexylglycerin 0.7g, phenoxyethanol 0.7g, water 50.39g.

[0032] Add 377, 577, arbutin, niacinamide, lactic acid bacteria fermentation product, morel extract, vitamin E and coenzyme Q10 to butanediol and stir until completely dissolved; then add glycerin, sodium hyaluronate, polyglutamic acid, betaine and hydrolyzed collagen, mix well and add to the remaining water, stir at 500 rpm / min for 20 min, centrifuge at 4°C and 4000 rpm / min for 10 min to remove bubbles, and obtain a viscous liquid; The viscous liquid is injected into the microneedle mold by vacuuming to ensure that the liquid is evenly distributed; after vacuuming, the excess viscous liquid on the surface of the mold is recovered to obtain a soluble microcrystalline needle tip; Take 10 parts of chitosan and 20 parts of hyaluronic acid solution, stir evenly, centrifuge at 4°C, 4000 rpm / min for 10 min to remove bubbles, and obtain a base solution; Adding the base solution to the soluble microcrystal needle tip, so that the base solution evenly covers the needle tip; Dry for 18 hours at a constant temperature of 35°C to 40°C and a humidity of 15% to 25%, and demold after drying to obtain soluble microcrystals with whitening and antioxidant functions.

[0033] Example 7 Weigh each component according to the following amount: 377 2g, 577 1g, arbutin 6.5g, niacinamide 8g, lactic acid bacteria fermentation product 4g, morel extract 1.5g, vitamin E 0.9g, coenzyme Q10 0.09g, glycerin 12g, butylene glycol 7g, sodium hyaluronate 0.25g, polyglutamic acid 0.9g, betaine 2.5g, hydrolyzed collagen 4g, squalane 7g, ethylhexylglycerin 1g, phenoxyethanol 0.8g, water 40.56g.

[0034] Add 377, 577, arbutin, niacinamide, lactic acid bacteria fermentation product, morel extract, vitamin E and coenzyme Q10 to butanediol and stir until completely dissolved; then add glycerin, sodium hyaluronate, polyglutamic acid, betaine and hydrolyzed collagen, mix well and add to the remaining water, stir at 500 rpm / min for 20 min, centrifuge at 4°C and 4000 rpm / min for 10 min to remove bubbles, and obtain a viscous liquid; The viscous liquid is injected into the microneedle mold by vacuuming to ensure that the liquid is evenly distributed; after vacuuming, the excess viscous liquid on the surface of the mold is recovered to obtain a soluble microcrystalline needle tip; Take 10 parts of chitosan and 20 parts of hyaluronic acid solution, stir evenly, centrifuge at 4°C, 4000 rpm / min for 10 min to remove bubbles, and obtain a base solution; Adding the base solution to the soluble microcrystal needle tip, so that the base solution evenly covers the needle tip; Dry for 18 hours at a constant temperature of 35°C to 40°C and a humidity of 15% to 25%, and demold after drying to obtain soluble microcrystals with whitening and antioxidant functions.

[0035] Example 8 Weigh each component according to the following amount: 377 1.5g, 577 0.7g, arbutin 5g, niacinamide 7g, lactic acid bacteria fermentation product 3.5g, morel extract 1.2g, vitamin E 0.7g, coenzyme Q10 0.07g, glycerin 11g, butylene glycol 6g, sodium hyaluronate 0.2g, polyglutamic acid 0.7g, betaine 2g, hydrolyzed collagen 3.5g, squalane 6g, ethylhexylglycerin 0.8g, phenoxyethanol 0.7g, water 49.43g.

[0036] Add 377, 577, arbutin, niacinamide, lactic acid bacteria fermentation product, morel extract, vitamin E and coenzyme Q10 to butanediol and stir until completely dissolved; then add glycerin, sodium hyaluronate, polyglutamic acid, betaine and hydrolyzed collagen, mix well and add to the remaining water, stir at 500 rpm / min for 20 min, centrifuge at 4°C and 4000 rpm / min for 10 min to remove bubbles, and obtain a viscous liquid; The viscous liquid is injected into the microneedle mold by vacuuming to ensure that the liquid is evenly distributed; after vacuuming, the excess viscous liquid on the surface of the mold is recovered to obtain a soluble microcrystalline needle tip; Take 10 parts of chitosan and 20 parts of hyaluronic acid solution, stir evenly, centrifuge at 4°C, 4000 rpm / min for 10 min to remove bubbles, and obtain a base solution; Adding the base solution to the soluble microcrystal needle tip, so that the base solution evenly covers the needle tip; Dry for 18 hours at a constant temperature of 35°C to 40°C and a humidity of 15% to 25%, and demold after drying to obtain soluble microcrystals with whitening and antioxidant functions.

[0037] Comparative Example 1 Weigh each component according to the following amounts: 377 1g, 577 0.5g, arbutin 3.5g, niacinamide 6g, lactic acid bacteria fermentation product 4g, vitamin E 0.5g, coenzyme Q10 0.05g, glycerin 10g, butylene glycol 5.5g, sodium hyaluronate 0.15g, polyglutamic acid 0.5g, betaine 1.5g, hydrolyzed collagen 3g, squalane 5g, ethylhexylglycerin 0.6g, phenoxyethanol 0.6g, water 57.6g.

[0038] Add 377, 577, arbutin, niacinamide, lactic acid bacteria fermentation product, morel extract, vitamin E and coenzyme Q10 to butanediol and stir until completely dissolved; then add glycerin, sodium hyaluronate, polyglutamic acid, betaine and hydrolyzed collagen, mix well and add to the remaining water, stir at 500 rpm / min for 20 min, centrifuge at 4°C and 4000 rpm / min for 10 min to remove bubbles, and obtain a viscous liquid; The viscous liquid is injected into the microneedle mold by vacuuming to ensure that the liquid is evenly distributed; after vacuuming, the excess viscous liquid on the surface of the mold is recovered to obtain a soluble microcrystalline needle tip; Take 10 parts of chitosan and 20 parts of hyaluronic acid solution, stir evenly, centrifuge at 4°C, 4000 rpm / min for 10 min to remove bubbles, and obtain a base solution; Adding the base solution to the soluble microcrystal needle tip, so that the base solution evenly covers the needle tip; Dry for 18 hours at a constant temperature of 35°C to 40°C and a humidity of 15% to 25%, and demold after drying to obtain soluble microcrystals with whitening and antioxidant functions.

[0039] Comparative Example 2 Weigh each component according to the following amounts: 377 1g, 577 0.5g, arbutin 3.5g, niacinamide 6g, morel extract 4g, vitamin E 0.5g, coenzyme Q10 0.05g, glycerin 10g, butylene glycol 5.5g, sodium hyaluronate 0.15g, polyglutamic acid 0.5g, betaine 1.5g, hydrolyzed collagen 3g, squalane 5g, ethylhexylglycerin 0.6g, phenoxyethanol 0.6g, water 57.6g.

[0040] Add 377, 577, arbutin, niacinamide, lactic acid bacteria fermentation product, morel extract, vitamin E and coenzyme Q10 to butanediol and stir until completely dissolved; then add glycerin, sodium hyaluronate, polyglutamic acid, betaine and hydrolyzed collagen, mix well and add to the remaining water, stir at 500 rpm / min for 20 min, centrifuge at 4°C and 4000 rpm / min for 10 min to remove bubbles, and obtain a viscous liquid; The viscous liquid is injected into the microneedle mold by vacuuming to ensure that the liquid is evenly distributed; after vacuuming, the excess viscous liquid on the surface of the mold is recovered to obtain a soluble microcrystalline needle tip; Take 10 parts of chitosan and 20 parts of hyaluronic acid solution, stir evenly, centrifuge at 4°C, 4000 rpm / min for 10 min to remove bubbles, and obtain a base solution; Adding the base solution to the soluble microcrystal needle tip, so that the base solution evenly covers the needle tip; Dry for 18 hours at a constant temperature of 35°C to 40°C and a humidity of 15% to 25%, and demold after drying to obtain soluble microcrystals with whitening and antioxidant functions.

[0041] Comparative Example 3 Weigh each component according to the following amounts: 377 1g, 577 0.5g, arbutin 3.5g, niacinamide 6g, lactic acid bacteria fermentation product 3g, morel extract 1g, vitamin E 0.5g, coenzyme Q10 0.05g, glycerin 10g, butylene glycol 5.5g, sodium hyaluronate 0.15g, polyglutamic acid 0.5g, betaine 1.5g, hydrolyzed collagen 3g, squalane 5g, ethylhexylglycerin 0.6g, phenoxyethanol 0.6g, water 57.6g.

[0042] Add 377, 577, arbutin, niacinamide, lactic acid bacteria fermentation product, morel extract, vitamin E and coenzyme Q10 to butylene glycol and stir until completely dissolved; then add glycerin, sodium hyaluronate, polyglutamic acid, betaine and hydrolyzed collagen, mix well, add to the remaining water, stir well to obtain a mixed solution.

[0043] Comparative Example 4 Weigh each component according to the following amounts: 377 1g, 577 0.5g, arbutin 3.5g, niacinamide 6g, lactic acid bacteria fermentation product 3g, morel extract 1g, vitamin E 0.5g, coenzyme Q10 0.05g, glycerin 10g, butylene glycol 5.5g, sodium hyaluronate 0.15g, polyglutamic acid 0.5g, betaine 1.5g, hydrolyzed collagen 3g, squalane 5g, ethylhexylglycerin 0.6g, phenoxyethanol 0.6g, water 57.6g.

[0044] Add 377, 577, arbutin, niacinamide, lactic acid bacteria fermentation product, morel extract, vitamin E and coenzyme Q10 to butylene glycol and stir until completely dissolved; then add glycerin, sodium hyaluronate, polyglutamic acid, betaine and hydrolyzed collagen, mix well, add to the remaining water, stir well to obtain a mixed solution.

[0045] The mixed solution was heated to 80°C and kept warm for 2 hours to allow all ingredients to fully dissolve, and then squalane was added. After stirring evenly, the system temperature was lowered to room temperature, and then ethylhexylglycerin and phenoxyethanol were added and stirred evenly.

[0046] After the system is cooled to room temperature, the pH value is adjusted to 5.5-6.5, and homogenized at 5000 rpm for 10 min using a homogenizer to obtain a whitening antioxidant essence.

[0047] Test Example 1 The products prepared in Examples 1 to 8 and Comparative Examples 1 to 4 were subjected to safety tests, and the specific methods are as follows: The patch tester with the test substance is applied to the flexor side of the subject's forearm with a non-irritating tape, and the tester is gently pressed with the palm of the hand to evenly apply it to the skin for 24 hours. Observe immediately after application, and repeat the observation and scoring at 24 hours, 48 ​​hours and 72 hours, and record possible symptoms such as erythema and edema and their degree.

[0048] The scoring criteria for the skin irritation test are as follows: Erythema and eschar formation: No erythema: 0 points Slight erythema (barely visible): 1 point Obvious erythema: 2 points Moderate to severe erythema: 3 points Severe erythema (purple red) to mild eschar formation: 4 points Edema formation: No edema: 0 points Slight edema (barely visible): 1 point Mild edema (clear skin contour): 2 points Moderate edema (skin swelling of about 1 mm): 3 points Severe edema (skin swelling greater than 1 mm, expanding): 4 points The maximum score for skin irritation reaction is 8 points. The skin irritation intensity is determined based on the average of the highest scores at the three observation time points of 24h, 48h and 72h: The average score is 6.0~8.0: strong irritation The average score is 2.0~<6.0: moderate irritation The average score is 0.5~<2.0: mild irritation The average score is 0~<0.5: non-irritating The results showed that the skin reactions of the subjects were all non-irritating, indicating that the products involved in the present invention are mild, safe and non-irritating.

[0049] Test Example 2 The products prepared in Examples 1 to 8 and Comparative Examples 1 to 4 were used as samples, and sample solutions of different concentrations were prepared respectively. In a 96-well plate, 100 μL of sample solution was added to each well. 100 μL of tyrosinase solution (0.1 U / mL) was added to each well. 100 μL of substrate solution (2 mM L-tyrosine or L-DOPA) was added to each well. Incubate at 37°C for 30 min. Measure the absorbance at a wavelength of 475 nm, and calculate the tyrosinase inhibition rate according to the following formula; Inhibition rate (%) = (1-absorbance of sample solution / absorbance of control group) × 100% Melanoma cells were seeded in 96-well plates, with 5,000 cells per well. Different concentrations of the test substance solution were added. The cells were incubated at 37°C and 5% CO 2 The cells were cultured for 48 hours under the same conditions. The intracellular melanin content was determined by spectrophotometry or image analysis technology, and the reduction rate of the melanin content was calculated according to the following formula. The results are shown in Table 1.

[0050] Reduction rate (%) = (1-melanin content in the sample group / melanin content in the control group) × 100% Table 1 Whitening effect verification test results

[0051] As can be seen from Table 1, compared with Comparative Examples 1 to 3, the products of Example 1 and Comparative Example 4 have higher inhibition rates on tyrosinase and melanin reduction rates, indicating that the lactic acid bacteria fermentation product and the morel extract have better whitening effects after combined use.

[0052] Test Example 3 1.DPPH free radical scavenging experiment The products prepared in Examples 1 to 8 and Comparative Examples 1 to 4 were used as samples, and sample solutions of different concentrations were prepared respectively.

[0053] In a 96-well plate, 100 μL of sample solution was added to each well, and then 100 μL of DPPH solution was added to each well.

[0054] After incubation at room temperature in the dark for 30 minutes, the absorbance was measured at a wavelength of 517 nm, and the DPPH free radical scavenging rate was calculated according to the following formula; the results are shown in Table 2.

[0055] Clearance rate (%) = (1-absorbance value of test substance / absorbance value of control group) × 100% 2. Oxygen free radical absorption capacity test The products prepared in Examples 1 to 8 and Comparative Examples 1 to 4 were used as samples, and sample solutions of different concentrations were prepared respectively.

[0056] In a 96-well plate, after adding 25 μL of sample solution to each well, 150 μL of AAPH solution (153 mM) was added to each well, and then 25 μL of fluorescein solution (81 nM) was added to each well.

[0057] The cells were incubated at 37°C in the dark and the fluorescence intensity was measured at an excitation wavelength of 485 nm and an emission wavelength of 528 nm every 15 min for a total of 120 min.

[0058] The reduction rate of fluorescence intensity was calculated according to the following formula and the results are shown in Table 2.

[0059] Reduction rate (%) = (1-fluorescence intensity of the test group / fluorescence intensity of the control group) × 100% Table 2 Antioxidant test results

[0060] As shown in Table 2, compared with Comparative Examples 1 and 2, the product of Example 1 has a higher DPPH free radical scavenging rate and a lower fluorescence intensity reduction rate, indicating that the composition and product provided by the present invention have better antioxidant effects.

[0061] Test Example 4 Transdermal absorption test Volunteers to be recruited: 120 people, female, aged between 18 and 50, healthy and not taking any medication.

[0062] Grouping: Volunteers were randomly divided into 12 groups, each with 10 people. Test groups 1-12 were given the products prepared in Example 1 to Example 8 and Comparative Examples 1 to Comparative Examples 4 in sequence, and a blank control area was set.

[0063] Test area: face.

[0064] Test instrument: The skin tester uses a skin water loss (TEWL) test probe.

[0065] Test samples and blank control: The sample application area and the blank control area were randomly distributed on the left and right sides of the face.

[0066] Measuring method: First measure the blank value of each test area, and then standardize the subjects to apply it evenly in the test area according to the prescribed usage, dosage and process.

[0067] Test time: 4 weeks. The changes in skin water loss content of the test area and the blank control area were measured before use, 2 weeks, and 4 weeks respectively. The moisturizing and barrier repair effects of the product were evaluated by measuring the TEWL value before and after use of the product and calculating the TEWL change rate according to the following formula. The results are shown in Table 3.

[0068] TEWL change rate (%) = (1-TEWL value after use / TEWL value before use) × 100% Table 3 TEWL change rate

[0069] It can be seen from Table 3 that compared with Comparative Examples 3 and 4, the products prepared in Examples 1 to 8 have a higher TEWL change rate, indicating that the products provided by the present invention can effectively inhibit the loss of skin moisture. The present invention uses microcrystalline technology to not only significantly improve the absorption efficiency of the product, but also give the product excellent moisturizing properties, and also excel in repairing the skin barrier.

[0070] Although the above embodiment describes the present invention in detail, it is only a part of the embodiments of the present invention, not all of the embodiments. People can also obtain other embodiments based on this embodiment without creativity, and these embodiments all fall within the protection scope of the present invention.

Claims

1. A whitening and antioxidant composition, characterized in that: The composition comprises the following components: 377 0.5wt%~2wt%, 577 0.1wt%~1wt%, arbutin 0.2wt%~7wt%, niacinamide 2wt%~10wt%, lactic acid bacteria fermentation product 1wt%~5wt%, morel extract 0.1wt%~2wt%, vitamin E 0.1wt%~1wt%, coenzyme Q10 0.01wt%~0.1wt%, glycerin 5wt%~15wt%, butylene glycol 3wt%~8wt%, sodium hyaluronate 0.05wt%~0.3wt%, polyglutamic acid 0.1wt%~1wt%, betaine 0.5wt%~3wt%, hydrolyzed collagen 1wt%~5wt%, squalane 2wt%~8wt%, ethylhexylglycerin 0.2wt%~1wt%, phenoxyethanol 0.3wt%~1wt% and the balance water.

2. The composition according to claim 1, characterized in that Includes the following components: 377 1wt%~2wt%, 577 0.5wt%~1wt%, arbutin 3wt%~5wt%, niacinamide 5wt%~8wt%, lactic acid bacteria fermentation product 2wt%~4wt%, morel extract 0.5wt%~1.5wt%, vitamin E 0.2wt%~0.5wt%, coenzyme Q10 0.02wt%~0.05wt%, glycerin 8wt%~12wt%, butylene glycol 5wt%~7wt%, sodium hyaluronate 0.1wt%~0.2wt%, polyglutamic acid 0.3wt%~0.5wt%, betaine 1wt%~2wt%, hydrolyzed collagen 2wt%~4wt%, squalane 4wt%~6wt%, ethylhexylglycerin 0.5wt%~0.8wt%, phenoxyethanol 0.5wt%~0.8wt% and the balance water.

3. The composition according to claim 1 or 2, characterized in that The arbutin is α-arbutin and / or β-arbutin.

4. The composition according to claim 3, characterized in that The added amount of the α-arbutin is 0.2wt%~2wt%, and the added amount of the β-arbutin is 1wt%~7wt%.

5. Use of the composition according to any one of claims 1 to 4 in the preparation of whitening and antioxidant products.

6. A whitening and antioxidant product, characterized in that: The product comprises the composition according to any one of claims 1 to 4.

7. The method for preparing the product according to claim 6, characterized in that: The following steps are involved: S1. Add 377, 577, arbutin, niacinamide, lactic acid bacteria fermentation product, morel extract, vitamin E and coenzyme Q10 to butanediol and stir until completely dissolved; then add glycerin, sodium hyaluronate, polyglutamic acid, betaine and hydrolyzed collagen, mix well and add to the remaining water, stir well, centrifuge to remove bubbles, and obtain a viscous liquid; S2, injecting the viscous liquid into the microneedle mold by vacuuming to ensure that the liquid is evenly distributed; after vacuuming, recovering the excess viscous liquid on the surface of the mold to obtain a soluble microcrystalline needle tip; S3, take 2-10 parts of chitosan and 4-20 parts of hyaluronic acid solution, stir evenly, and centrifuge to remove bubbles to obtain a base solution; S4, adding the base solution to the soluble microcrystal needle tip, so that the base solution evenly covers the needle tip; S5. Dry for 12 to 24 hours at a constant temperature of 35°C to 40°C and a humidity of 15% to 25%. De-mold after drying to obtain soluble microcrystals with whitening and antioxidant functions.

8. The preparation method according to claim 7, characterized in that: The stirring rate is 500 rpm / min, and the stirring time is 15-40 min.

9. The preparation method according to claim 7, characterized in that: The step of removing bubbles by centrifugation is: centrifugation at 4000 rpm / min for 10 min at 4°C.

10. The preparation method according to claim 7, characterized in that: The molecular weight of the chitosan is 240,000 Da, and the molecular weight of the sodium hyaluronate is 100,000 Da.

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