Traditional Chinese and western medicine combined honeyed bolus for clearing away heat and toxic materials and tonifying qi and spleen for pigs and preparation method of honeyed bolus

Abstract

The invention belongs to the technical field of traditional Chinese and western combined medicines, and particularly relates to a traditional Chinese and western combined honeyed pill for clearing away heat and toxic materials and replenishing qi to invigorate the spleen for pigs and a preparation method of the traditional Chinese and western combined honeyed pill, and the traditional Chinese and western combined honeyed pill comprises the following components in parts by weight: 10-15 parts of enrofloxacin; 25 to 35 parts of cortex phellodendri; 25 to 35 parts of clove; 20-30 parts of radix aucklandiae; 20-30 parts of coptis chinensis; 15 to 20 parts of fructus gardeniae; 15-20 parts of pericarpium citri reticulatae; and 70-115 parts of honey. According to the present invention, the pig digestive tract infection symptom can be rapidly treated, the influence of the enrofloxacin, the coptis chinensis, the phellodendron amurense and other drugs with extremely low bitter taste on the pig ingestion can be completely eradicated, the effects of stomach invigorating and appetite enhancing are provided, the clinical test results of the formula show that the pig heat clearing effect is equivalent to the conventional heat clearing and pain relieving drug, and the expected effect is achieved.

Description

Technical Field

[0001] The invention belongs to the technical field of Chinese and Western medicine combination, and particularly relates to a Chinese and Western medicine combination honey pill for clearing away heat and detoxifying, invigorating qi and strengthening spleen in pigs and a preparation method thereof. Background Art

[0002] The combined use of Chinese and Western medicines can be traced back to the combined treatment of "epidemic fever" with aspirin and Baihu decoction. The combination of Chinese and Western medicines is now quite common in veterinary clinics. For example, honeysuckle can enhance the bactericidal effect of penicillin on drug-resistant Staphylococcus aureus; Citrus aurantium can relax the bile sphincter, allowing gentamicin to enter the bile duct and improve the anti-infection effect; hydroxybutyrophenone in Artemisia capillaris decoction promotes bile secretion, increases the solubility and absorption rate of griseofulvin, and thus enhances its antibacterial effect.

[0003] As we all know, Western medicine usually has clear pharmacological effects and a fast onset time, which can quickly control the disease; while Chinese medicine focuses on overall regulation, which can improve the systemic symptoms of the animal body and enhance the body's resistance. The rational prescription of Chinese and Western medicines can improve the efficacy, reduce the dosage and toxic side effects of chemical and Chinese medicines, expand the scope of indications, shorten the course of treatment and promote physical recovery, and can play a therapeutic role that cannot be achieved by using Chinese or Western medicine alone.

[0004] In addition, a single drug may have obvious side effects when used for a long time. For example, coptis root, phellodendron, and scutellaria are bitter and cold in nature, and long-term use can easily damage the stomach and cause gastrointestinal reactions; quinolone drugs such as enrofloxacin can also cause gastrointestinal reactions, decreased appetite, and hepatotoxicity, causing liver and kidney damage to animals, etc. It is an important topic in breeding production that drugs used to treat diseases can also strengthen the stomach and promote appetite.

[0005] At the same time, enrofloxacin, coptis root, phellodendron, and scutellaria have a common weakness, which is that the bitterness value is extremely low. Pigs often refuse to eat feeds added with enrofloxacin or coptis root, phellodendron, scutellaria and other drugs. The research on taste masking has been continuously deepened at home and abroad, and certain progress has been made, but the taste correction effect of the preparation is still poor, and the production requirements are high. The existing taste masking technologies have certain limitations. Most technologies, such as coating and microcapsules, effectively mask the bitterness of the drug by physical methods. Once the masking components are broken, the bitter drug will be released in the animal's mouth. Summary of the invention

[0006] In view of the deficiencies in the prior art, the present invention provides a Chinese and Western medicine combined honey pill for clearing away heat and detoxifying, invigorating qi and strengthening the spleen for pigs and a preparation method thereof. The pill can quickly treat the symptoms of digestive tract infection in pigs, can completely eliminate the influence of drugs with extremely low bitterness values ​​such as enrofloxacin, coptis root, and phellodendron on pigs' feeding, and has the effect of strengthening the stomach and enhancing appetite. At the same time, after clinical trials of the formula, it was found that the pill has an effect on relieving fever in pigs that is equivalent to that of conventional antipyretic and analgesic drugs, achieving unexpected effects.

[0007] To achieve the above objectives, the present invention is implemented through the following technical solutions: A Chinese and Western medicine combined honey pill for clearing away heat and detoxifying, invigorating qi and strengthening the spleen for pigs, comprising the following components by weight: Enrofloxacin 10-15 parts; 25-35 parts of Phellodendron chinense; 25-35 parts of cloves; 20-30 parts of costus root; 20-30 parts of Coptis chinensis; 15-20 parts of Gardenia; 15-20 parts dried tangerine peel; 70-115 parts of honey.

[0008] A method for preparing the Chinese and Western medicine combined honey pills as claimed in claim 1, comprising the following steps: S1: Take Chinese herbal medicine pieces of Phellodendron chinense, Clove, Aucklandia lappa, Coptis chinensis, Gardenia jasminoides, and Tangerine peel, dry them at 80°C for 4h, mix them, grind them in a grinder, and pass them through a 50-mesh sieve to obtain powder A; S2: Add powder A into a superfine grinder and crush it into 200 mesh to obtain powder B; S3: Take enrofloxacin powder and mix it into powder B by equal amount adding method until the enrofloxacin powder is completely mixed to obtain powder C; S4: Heat the honey in a pot and stir it at any time to remove the floating objects and foam until the honey is refined for 10-15 minutes and becomes viscous old honey; S5: Put the C powder into the lifting mixer and mix for 30 minutes, then take it out, mix the refined honey with the medicine powder evenly, combine the medicine, make strips, and make pills, and finally screen it with a 10-mesh sifter to obtain the Chinese and Western medicine combined honey pills.

[0009] The present invention has the following beneficial effects: 1. The present invention omits scutellaria baicalensis, reduces the dosage of phellodendron chinense, coptis chinensis and gardenia jasminoides on the basis of the prescription of coptis chinensis, scutellaria baicalensis, phellodendron chinense and gardenia jasminoides, and adds enrofloxacin, cloves, costusroot, tangerine peel and honey. The clinical effect of the present invention on treating digestive tract infection in pigs is better than that of the whole Chinese medicine prescription of coptis chinensis and the whole Western medicine prescription of enrofloxacin with the same dosage.

[0010] 2. The present invention is supplemented with an appropriate amount of honey to make honey pills, which can completely eliminate the effects of extremely low bitterness drugs such as enrofloxacin, coptis chinensis, and phellodendron on pigs' feeding. The existing taste masking technology mainly uses microcapsules or microspheres to cover the bitterness of drugs by physical methods, and the taste correction effect is often poor, and the production requirements are high. The taste masking of the present invention has three layers of technical protection: the first layer of technology uses tangerine peel, cloves, costus root, gardenia and bitter drugs phellodendron, coptis chinensis, and enrofloxacin to mix together, and then mixes with honey to make honey pills with good taste, eliminate the bitterness of enrofloxacin, coptis chinensis, and phellodendron, and is not easy to be crushed by pigs to release bitterness. The second layer of technology is that the release of bitter drugs under extreme conditions can also be neutralized by the special flavor of honey and tangerine peel. The third layer of technology is that the main components of cloves, such as eugenol and chavicol, have paralytic components, and the animal taste buds are temporarily and reversibly paralyzed, and the taste of the drug cannot be felt in a short time.

[0011] 3. The drug combination of the present invention not only prevents pigs from losing their appetite, but also has an obvious stomach-strengthening and appetite-promoting effect. The optimized clove component is an aromatic stomachic agent that can warm the stomach and dispel cold, and can offset the spleen and stomach deficiency and coldness caused by taking Phellodendron chinense and Coptis chinensis; costus root can improve intestinal peristalsis and treat spleen and stomach qi stagnation; tangerine peel is aromatic and dehumidifies, promotes qi, strengthens the spleen and harmonizes the stomach; honey lubricates the intestines and relieves constipation, normalizes gastric acid secretion, and regulates gastrointestinal function.

[0012] 4. The unexpected antipyretic effect of the present invention is that the rational formulation has played a therapeutic role that cannot be achieved by using Chinese medicine or Western medicine alone, stimulating Gardenia to inhibit the body temperature center to play an antipyretic effect. In addition, the laxative and moistening honey may have stimulated the heat-clearing and antipyretic effects of Phellodendron chinense and Coptis chinensis.

[0013] 5. The Chinese and Western medicine honey pill dosage form produced by the present invention is rarely used in the veterinary field. In addition, the process is relatively simple, the production time is short, and it is easy to realize large-scale intelligent production. DETAILED DESCRIPTION

[0014] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0015] Embodiment 1: A Chinese and Western medicine combined honey pill for clearing away heat and detoxifying, invigorating qi and strengthening the spleen in pigs is prepared from the following components by weight: 25g each of phellodendron and clove, 20g each of costusroot and coptis root, 15g each of gardenia and tangerine peel, 10g of enrofloxacin and 70g of honey.

[0016] The preparation method comprises the following steps: S1: Take Chinese herbal medicine pieces of Phellodendron chinense, Clove, Aucklandia lappa, Coptis chinensis, Gardenia jasminoides, and Tangerine peel, dry them at 80°C for 4h, mix them, grind them in a grinder, and pass them through a 50-mesh sieve to obtain powder A; S2: Add powder A into a superfine grinder and crush it into 200 mesh to obtain powder B; S3: Take enrofloxacin powder and mix it into powder B by equal amount adding method until the enrofloxacin powder is completely mixed to obtain powder C; S4: Heat the honey in a pot and stir it at any time to refine it, remove the floating objects and foam, and refine the honey for 10-15 minutes until it becomes viscous old honey. Appropriately reduce the water content and increase the adhesion to meet the requirements of pill making; S5: Put the C powder into the lifting mixer and mix for 30 minutes, then take it out, mix the refined honey with the medicine powder evenly, combine the medicine, make strips, and make pills, and finally screen it with a 10-mesh sifter to obtain the Chinese and Western medicine combined honey pills.

[0017] Embodiment 2: The formula of this embodiment is made of the following components by weight: 30g each of Phellodendron chinense and Cloves, 25g each of Aucklandia lappa and Coptis chinensis, 17.5g each of Gardenia jasminoides and Tangerine peel, 12.5g of Enrofloxacin, and 92.5g of Honey.

[0018] The preparation method is the same as that in Example 1.

[0019] Embodiment 3: The formula of this embodiment is made of the following components by weight: 35g each of Phellodendron chinense and Cloves, 30g each of Aucklandia lappa and Coptis chinensis, 20g each of Gardenia jasminoides and Tangerine peel, 15g of Enrofloxacin, and 115g of Honey.

[0020] The preparation method is the same as that in Example 1.

[0021] 1. Stability test of the Chinese and Western combination honey pills obtained in Examples 1-3 1. Materials and methods: Take the Chinese and Western medicine combination honey pills of Examples 1-3, and perform a high temperature, high humidity and strong light accelerated test according to the drug stability experimental method in Appendix 1 of the 2020 edition of the "Chinese Pharmacopoeia of Veterinary Medicine". According to the proposed commercial packaging, place them in a drug stability test box (Chongqing Yongsheng Experimental Instrument Factory) at a temperature of 40±2°C, a relative humidity of 75±5%, and an illumination of 4500±500LX for 6 months. Samples were taken at the end of the 1st, 2nd, 3rd, and 6th month during the experiment to investigate the properties, content, and particle size of the honey pills.

[0022] 2. Results: See Table 1.

[0023] Table 1: Stability test data of Chinese and Western medicine combined honey pills As shown in Table 1, after 6 months of accelerated testing, the properties, particle size and content of each component of the Chinese and Western medicine combination honey pills of the present invention had no significant changes, and the stability was good.

[0024] 2. Effect of Chinese and Western medicine combined honey pills on the treatment of transmissible gastroenteritis in piglets 1. Materials and Methods 1.1 Drugs Experimental group: Chinese and western medicine combined honey pills, using the sample of Example 2 of the present invention.

[0025] Control group 1: Enrofloxacin soluble powder, specification: 5%, produced by a veterinary drug factory in Chongqing.

[0026] Control group 2: Huanglian Jiedu Powder, produced by a veterinary drug factory in Chongqing.

[0027] 1.2 Source of cases: Technical personnel collected 615 clinical natural disease cases in Hechuan, Bishan and other areas.

[0028] 1.3 Case diagnosis: Natural cases are diagnosed by laboratory according to epidemiology, clinical symptoms, pathological changes. Comprehensive analysis is used to confirm the diagnosis. Pathological manifestations include loss of appetite, severe watery or pasty diarrhea, yellow feces, often with undigested curds, foul odor, rapid weight loss, and obvious dehydration of piglets.

[0029] 1.4 Implementation process: The technical staff of this research group will select cases and implement treatment according to the test requirements and record the test results.

[0030] 1.5 Result determination The natural cases were given the same dosage and course of treatment by drinking water, and the patients were observed and visited again within one week to record their condition. Case judgment criteria: Cure: After 3-4 doses of the drug, the symptoms of the affected animals completely disappeared, their spirit, body temperature, and appetite returned to normal, and their urine and feces were normal.

[0031] Effective: After 3-4 doses of the drug, the symptoms of the affected animals basically disappeared, their spirit, body temperature, and appetite improved, and their urine and feces became normal.

[0032] Ineffective: The symptoms of the affected animals did not disappear after 4 doses of medication, the condition worsened or the animal died, and the color and properties of urine and feces were abnormal.

[0033] 2. Results: See Table 2.

[0034] Table 2: Statistical table of clinical treatment effects of Chinese and Western medicine combined honey pills It can be seen from Table 2 that the Chinese and Western medicine combined honey pills of the present invention have a good therapeutic effect. The effective rate of piglet transmissible gastroenteritis is 16.6% higher than that of the traditional Western medicine control group 1 and 14.6% higher than that of the traditional Chinese medicine control group 2; the cure rate of piglet transmissible gastroenteritis is 36.2% higher than that of the traditional Western medicine control group 1 and 41.7% higher than that of the traditional Chinese medicine control group 2. The improvement in cure rate is very obvious, which shows that the Chinese and Western medicine combined honey pills with optimized formula have better clinical effect in treating pig digestive tract infections.

[0035] 3. Effect of Chinese and Western medicine combined honey pills on masking taste and increasing feed intake 1. Methods and Materials 1.1 Preparation of medicines and feed.

[0036] Test group: Chinese and Western medicine combined honey pills, using the sample of Example 3 of the present invention. 1g of the drug was added to every 1kg of feed, and mixed for 20 minutes according to the daily mixed feeding process.

[0037] Control group 1: Enrofloxacin soluble powder (microcapsule taste masking technology product), specification: 5%, produced by a veterinary drug factory in Chongqing. Add 1g of this drug to every 1kg of feed and mix for 20 minutes according to the daily mixing process.

[0038] Control group 2: Huanglian Jiedusan, produced by a veterinary drug factory in Chongqing. Add 1g of this drug to every 1kg of feed and mix for 20 minutes according to the daily mixing process.

[0039] Blank group: The mice were fed normally without adding any drugs.

[0040] 1.2 Experimental animals: 800 healthy and normal pig nursery pigs were randomly selected from the experimental pig farm and randomly divided into 4 groups for separate feeding. After separation, the pigs were allowed to eat freely for 3 days before starting the experiment to avoid the emergency effects that may be caused by the separation.

[0041] 1.3 Implementation process At the beginning of the trial, the feed trough was emptied, and a fixed amount of mixed medicated feed or blank feed was added every day. The remaining feed in the trough was removed every 24 hours, and the average daily feed intake of each group was weighed and calculated. The average daily feed intake = (weight of feed added to each group - weight of remaining feed removed) / number of pigs in each group. The trial lasted for 7 days.

[0042] 2. Experimental data table results are shown in Table 3 Table 3: Experimental data of average daily feed intake of growing pigs Example 3: Adding Chinese and Western medicine combined honey pills can significantly improve the feed intake level of nursery pigs, with a significant increase in feed intake from the 5th day, and a 9.8% increase in feed intake on the 7th day compared to the first day.

[0043] Control group 1 added enrofloxacin microcapsules using traditional taste-masking technology, which significantly reduced feed intake from the 4th day. On the 6th day, the feed intake decreased by 37.5% compared with the first day. On the 7th day, the pigs basically refused to eat.

[0044] In the control group 2, the addition of Huanglian Jiedu Powder significantly reduced the feed intake from the 5th day, and on the 7th day the feed intake was reduced by 24.1% compared with the first day. No food refusal occurred within 7 days.

[0045] The blank group was a blank feed without any drugs added. The feed intake was balanced within 7 days, with a fluctuation rate of less than 1.5%, and the test data was credible.

[0046] 4. Comparison of the antipyretic effect of the combined Chinese and Western medicine honey pills and the rapid antipyretic drug Bupleurum injection.

[0047] 1. Methods and Materials 1.1 Drugs The Chinese and Western medicine combined honey pills adopt the samples of Examples 1, 2 and 3 of the present invention.

[0048] Chaihu injection is produced by a veterinary drug factory in Chongqing and is sold on the market and has passed inspection and is a qualified product.

[0049] 0.9% normal saline, prepared in the laboratory.

[0050] Typhoid attenuated vaccine was purchased from China National Biotechnology Group Corporation.

[0051] 1.2 Experimental animals: Fifty healthy rabbits weighing 1.5-2.0 kg and with a body temperature of 39.0±5℃ for 3 consecutive days were randomly divided into 5 groups, with 10 rabbits in each group, half of which were male and half were female.

[0052] 1.3 Implementation process: The first three groups of rabbits were respectively given oral administration of 1g / kg Chinese and Western medicine combination honey pills Example 1, Example 2 and Example 3, and the control group was injected with Bupleurum injection, 1ml per injection. The blank group was injected with 0.9% normal saline, 1ml per injection. After 30 minutes of injection, each group of rabbits was injected with 1ml / kg ear vein typhoid attenuated vaccine, and then the rectal temperature was measured 30, 60, 90, 120, 150, 180, 210, 240min before and after the injection of the vaccine, and the data were recorded. The results are shown in Table 4.

[0053] 2. The results are shown in Table 4 Table 4. Results of antipyretic test in febrile rabbits (℃, ±S) It can be seen from Table 4 that 30 minutes after the injection of the attenuated typhoid vaccine, the body temperature of the rabbits in each group increased significantly and reached a peak at 60 minutes. The Chinese and Western medicine combination honey pills of Examples 1 to 3 basically controlled the body temperature of the sick rabbits to normal at 150 minutes, while the Bupleurum injection basically controlled the body temperature of the sick rabbits to normal at 120 minutes. The temperature fluctuation difference of the Chinese and Western medicine combination honey pills of the present invention is also small in each time period, and the antipyretic function can basically achieve the effect of using Bupleurum injection mainly for fever symptoms.

[0054] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A Chinese and Western medicine combined honey pill for clearing away heat and detoxifying, invigorating qi and strengthening the spleen for pigs, characterized in that: The following components are included by weight: Enrofloxacin 10-15 parts; 25-35 parts of Phellodendron chinense; 25-35 parts of cloves; 20-30 parts of costus root; 20-30 parts of Coptis chinensis; 15-20 parts of Gardenia; 15-20 parts dried tangerine peel; 70-115 parts of honey.

2. A method for preparing the Chinese and Western medicine combined honey pills as claimed in claim 1, characterized in that: The following steps are involved: S1: Take Chinese herbal medicine pieces of Phellodendron chinense, Clove, Aucklandia lappa, Coptis chinensis, Gardenia jasminoides, and Tangerine peel, dry them at 80°C for 4h, mix them, grind them in a grinder, and pass them through a 50-mesh sieve to obtain A powder; S2: Add powder A into a superfine grinder and crush it into 200 mesh to obtain powder B; S3: Take enrofloxacin powder and mix it into powder B by equal amount adding method until the enrofloxacin powder is completely mixed to obtain powder C; S4: Heat the honey in a pot and stir it at any time to remove the floating objects and foam until the honey is refined for 10-15 minutes and becomes viscous old honey; S5: Put the C powder into the lifting mixer and mix for 30 minutes, then take it out, mix the refined honey with the medicine powder evenly, combine the medicine, make strips, and make pills, and finally screen it with a 10-mesh sifter to obtain the Chinese and Western medicine combined honey pills.

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