Mixing equipment for cell experiment and use method of mixing equipment
By designing a cell experimental mixing equipment including support unit and mixing unit, problems such as uneven mixing, mechanical damage, and contamination risks in existing devices are solved, and effective mixing and fixing of test tubes of different lengths is achieved, improving mixing efficiency and simplicity of operation.
Patent Information
- Application Number
- CN202510158957.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-02-13
- Publication Date
- 2025-05-13
AI Technical Summary
The existing cell experimental mixing devices have problems such as uneven mixing, mechanical damage, risk of pollution, equipment failure, complex operation and high maintenance costs, and are especially unable to adapt to test tubes of different lengths.
A cell experimental mixing device including a support unit and a mixing unit is designed. The support unit realizes stable fixation of the test tube through the floating ring sleeve and the central pillar. The mixing unit uses floating clips, rebound caps and mixing rods to move up and down and rotate the mixing rod through the height adjustment of the floating ring sleeve and pressing the rebound cap to improve mixing efficiency.
Through the design of floating ring sleeves and mixing rods, effective fixation and mixing of test tubes of different lengths is achieved, which improves the uniformity and efficiency of cell mixing, and reduces operational complexity and maintenance costs.
Smart Images
Figure CN119971847A_ABST
Abstract
Description
Technical Field
[0001] The invention relates to the technical field of cell mixing, and in particular to a mixing device for cell experiments and a use method thereof. Background Art
[0002] Cell experiment mixing devices are devices used to mix samples in cell experiments. Common ones include vortex mixers, rotary mixers, etc. Vortex mixers use vortex flow to quickly mix samples. They are suitable for mixing cell culture media, dissolving cell membranes, and breaking cells. Rotary mixers use a rotating platform to fully mix the components in the test tube sample. They are simple to operate and have good mixing effects. They are suitable for a variety of experimental scenarios such as PCR experiments and cell culture.
[0003] The existing cell experiment mixing devices have the following problems during use: uneven mixing, resulting in uneven cell distribution, affecting the accuracy and repeatability of experimental results; mechanical damage, when the mixing speed is too fast or the force is too strong, it is easy to damage cells, affecting activity and growth status; contamination risk, incomplete cleaning and disinfection of the device or improper operation, it is easy to cause cell contamination; equipment failure, such as vortex mixer motor failure, unstable rotary mixer speed, etc., affecting the mixing effect and experimental progress; complex operation, requiring professional training and operating skills, increasing the difficulty of the experiment and the probability of error; high maintenance cost, some devices require regular maintenance and replacement of parts, increasing experimental costs and maintenance workload. In addition, the existing mixing devices cannot be adapted and fixed for test tubes of different lengths. For example, some devices are only suitable for test tubes of a specific length, and cannot effectively fix and mix test tubes of different lengths, affecting the versatility and flexibility of the experiment. Summary of the invention
[0004] In view of the problems that the above-mentioned existing mixing device has uneven mixing and cannot adapt to test tubes of different lengths, the present invention is proposed.
[0005] Therefore, the object of the present invention is to provide a mixing device for cell experiments and a method of using the same, the purpose of which is to improve the cell mixing efficiency and meet the mixing requirements of cell solutions in test tubes of different lengths.
[0006] In order to solve the above technical problems, the present invention provides the following technical solutions: a mixing device for cell experiments, comprising: a supporting unit, including a base, a bottom ring sleeve, a floating ring sleeve and a central pillar, the central pillar is fixedly arranged in the middle of the base, the bottom ring sleeve is fixedly connected to the top of the base, and the floating ring sleeve is movably connected to the central pillar; a mixing unit, including a floating clamp, a rebound cap and a mixing rod, the floating clamp is movably arranged in the central pillar and fixed to the floating ring sleeve, the rebound cap is movably arranged on the top of the central pillar, and the mixing rod is arranged in a plurality of groups and is circumferentially fixed outside the rebound cap.
[0007] As a preferred solution of the mixing device for cell experiments of the present invention, there is a plurality of bottom brackets fixedly connected to the top of the base in a circumferential direction; a plurality of horizontal plates are fixedly connected to the outer wall of the bottom ring sleeve at equal intervals, and vertical plates are vertically fixedly connected to the bottom of the horizontal plates, and the vertical plates are fixedly connected to the base through connecting ears; a plurality of first fixing holes are also provided in the bottom ring sleeve, and the first fixing holes are arranged circumferentially, and test tubes are fitted and inserted in the first fixing holes, and the bottoms of the test tubes are fitted and placed on the bottom brackets.
[0008] As a preferred solution of the mixing device for cell experiments of the present invention, wherein: a plurality of groups of second fixing holes are opened in the floating ring sleeve, the second fixing holes are aligned with the first fixing holes, and the test tube can be plugged into the second fixing holes; docking plates are symmetrically fixed on the inner wall of the floating ring sleeve, the end of the docking plate is fixedly connected to a connecting cover shell, the connecting cover shell is slidably sleeved outside the central pillar, and the top of the connecting cover shell is fixedly connected to a top plate.
[0009] As a preferred solution of the mixing device for cell experiments of the present invention, a rectangular through-hole is provided in the central pillar, and both ends of the rectangular through-hole are connected to the outside world through fan-shaped through-holes; a plurality of groups of slots are longitudinally and equidistantly provided on the inner walls on the opposite sides of the rectangular through-hole; a sliding column is fixedly connected to the top of the central pillar, and sliding slots are symmetrically provided on the outer wall of the sliding column, and an anti-drop cap is fixedly connected to the top of the sliding column.
[0010] As a preferred solution of the mixing device for cell experiments of the present invention, the floating clamp comprises a first fan-shaped block, a rectangular block and a second fan-shaped block, one end of the rectangular block is fixedly connected to the second fan-shaped block, and the other end is detachably fixed to the first fan-shaped block, and the outer walls of the first fan-shaped block and the second fan-shaped block are detachably fixedly connected to the connecting cover shell; the first fan-shaped block and the second fan-shaped block slide in the fan-shaped perforations on both sides respectively; a rectangular groove is provided on the top of the rectangular block, a rebound groove is provided at one end of the first fan-shaped block close to the rectangular block, a sliding slot is provided in the middle of the second fan-shaped block, a clamping piece is centrally symmetrically arranged in the rectangular groove, and an unlocking piece is slidably inserted in the sliding slot.
[0011] As a preferred solution of the mixing device for cell experiments of the present invention, the top opening of the rectangular block is sealed and detachably fixed with a top shell, the middle part of the bottom end of the top shell is rotatably connected with a rotating shaft, and a gear is fixedly connected to the outside of the rotating shaft; the clamping part includes a vertical plate, an anti-bias slider, a card block and a docking tooth plate, the card block is fixedly connected to the middle part of one side of the vertical plate, the docking tooth plate is fixedly connected to the other side of the vertical plate, and the docking tooth plates in the clamping parts on both sides are simultaneously meshed and connected with the gear in the middle; the outer wall of the rectangular block is symmetrically provided with external connection holes, the card block slides through the external connection holes, and is inserted into the card slot, the anti-bias slider is fixedly connected to the bottom of the vertical plate, and the anti-bias slider is placed in the rectangular groove in a sliding manner.
[0012] As a preferred solution of the mixing device for cell experiments of the present invention, the unlocking part includes a sliding block, a pressing plate, a clamping plate and a rebound plate, and the sliding block is slidably inserted into the sliding slot; the pressing plate is fixedly connected to one end of the sliding block, and the clamping plate is symmetrically fixed to the other end of the sliding block, and a plurality of groups of racks are connected to the inner wall of one side of the clamping plate, and the racks are meshed with the gears; the rebound plate is fixedly connected to the end of the clamping plate, and the rebound plate can be slidably inserted into the rebound slot, and a first spring is fixedly connected between the rebound plate and the inner wall of the rebound slot.
[0013] As a preferred solution of the mixing device for cell experiments of the present invention, the rebound cap comprises a pressing cap and a rebound sleeve coaxially fixedly connected to its bottom; a plurality of groups of cross bars are equidistantly fixedly connected to the outer wall of the bottom of the pressing cap, a clamping ring is fixedly connected to the end of the cross bar, a mounting hole is opened in the middle of the clamping ring, and push-clip grooves are also opened on both sides of the mounting hole, a buckle is slidably connected in the push-clip groove, and a second spring is fixedly connected between the buckle and the inner wall of the push-clip groove; the rebound sleeve comprises a sleeve clamping ring, the top and bottom ends of the sleeve clamping ring are coaxially fixedly connected with an insert tube and a rebound ring, the inner wall of the sleeve clamping ring is symmetrically fixedly connected with a clamping strip, the sleeve clamping ring is slidably sleeved on the sliding column, and the clamping strip is slidably inserted into the sliding slot; a third spring is also fixedly connected between the rebound ring and the top plate, and the third spring is sleeved outside the central pillar.
[0014] As a preferred solution of the mixing device for cell experiments of the present invention, the mixing rod includes a connecting rod and a rotating rod, the outer wall of the connecting rod is symmetrically provided with height adjustment grooves, the connecting rod is slidably inserted into the mounting hole, and the buckle is slidably inserted into the height adjustment groove; the bottom end of the connecting rod is also connected to a rotating drum, the top end of the rotating drum is rotatably sleeved on the connecting rod, and the top end of the rotating rod is fixedly inserted in the middle of the bottom end of the rotating drum; the outer wall of the bottom end of the rotating rod is connected to a plurality of groups of stirring blades, the edges of the stirring blades are fixedly connected to a convergence tube, and the convergence tube is rotatably inserted into the test tube.
[0015] In order to solve the above technical problems, the present invention provides the following technical solutions: a method for using a mixing device for cell experiments, which is based on the above-mentioned mixing device for cell experiments, and includes the following steps: first, injecting various types of cell solutions to be mixed into a test tube, and placing the test tube into a bottom ring sleeve and a floating ring sleeve; adjusting the height of the floating ring sleeve to ensure that the floating ring sleeve is always below the top opening of the test tube to prevent the test tube from tipping over; adjusting the height of the mixing rod to ensure that the stirring component at the bottom of the mixing rod extends into the cell mixed solution; pressing the rebound cap with a finger, at which time the height of the rebound cap is reduced, driving the mixing rod to descend and stir the cell mixed solution, and when the mixing rod descends, it will be rotated by the cell mixed solution, and rotate synchronously in a circumferential direction to improve the cell mixing efficiency.
[0016] Compared with the prior art, the present invention has the following beneficial effects:
[0017] The floating ring can be adjusted freely in height by means of the floating clamp, ensuring that the floating ring and the bottom ring can fix the test tube from above and below, preventing the test tube from being too long and tipping over due to improper clamping of the floating ring. By pressing the rebound cap, the mixing rods on all sides can be driven to move up and down synchronously, so that the mixing rods stir the cell solution up and down in the test tube, and multiple groups of samples can be mixed at the same time. When the mixing rods stir the solution up and down, they will automatically rotate under the resistance of the solution, further stirring the solution circumferentially, thereby improving the mixing efficiency. BRIEF DESCRIPTION OF THE DRAWINGS
[0018] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following briefly introduces the drawings required for describing the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work. Among them:
[0019] Figure 1 This is a schematic diagram of the overall structure of the mixing device for cell experiments of the present invention.
[0020] Figure 2 This is a schematic diagram of the support unit structure of the mixing device for cell experiments of the present invention.
[0021] Figure 3 This is a schematic diagram of the floating ring structure of the mixing device for cell experiments of the present invention.
[0022] Figure 4 This is a schematic diagram of the floating card structure of the mixing device for cell experiments of the present invention.
[0023] Figure 5 This is a schematic diagram of the connection between the clamping part and the unlocking part of the mixing device for cell experiments of the present invention.
[0024] Figure 6This is a schematic diagram of the external structure of the floating clamp of the mixing device for cell experiments of the present invention.
[0025] Figure 7 This is a schematic diagram of the rebound cap structure of the mixing device for cell experiments of the present invention.
[0026] Figure 8 A cross-sectional view of the clamp ring of the mixing device for cell experiments of the present invention.
[0027] Fig. 9 A cross-sectional view of a mixing rod of a mixing device for cell experiments of the present invention.
[0028] Fig.10 This is a diagram showing the changing state of the mixing device used in the cell experiment of the present invention. DETAILED DESCRIPTION
[0029] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the specific implementation methods of the present invention are described in detail below in conjunction with the accompanying drawings.
[0030] In the following description, many specific details are set forth to facilitate a full understanding of the present invention, but the present invention may also be implemented in other ways different from those described herein, and those skilled in the art may make similar generalizations without violating the connotation of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.
[0031] Secondly, the term "one embodiment" or "embodiment" as used herein refers to a specific feature, structure, or characteristic that may be included in at least one implementation of the present invention. The term "in one embodiment" that appears in different places in this specification does not necessarily refer to the same embodiment, nor does it refer to a separate or selective embodiment that is mutually exclusive with other embodiments.
[0032] Secondly, the present invention is described in detail with reference to the schematic diagram. When describing the embodiments of the present invention in detail, for the sake of convenience, the cross-sectional diagrams showing the device structure will not be partially enlarged according to the general scale, and the schematic diagrams are only examples, which should not limit the scope of protection of the present invention. In addition, in actual production, the three-dimensional dimensions of length, width and depth should be included.
[0033] Example 1
[0034] Reference Figures 1 to 10 , which is the first embodiment of the present invention. In this embodiment, the culture conditions of cells are an external temperature of 37°C and a CO2 content of 5%, and a mixing device for cell experiments is provided, which includes a support unit 1, including a base 11, a bottom ring sleeve 12, a floating ring sleeve 13 and a central pillar 14. The central pillar 14 is fixedly arranged in the middle of the base 11, the bottom ring sleeve 12 is fixedly connected to the top of the base 11, and the floating ring sleeve 13 is movably connected to the central pillar 14.
[0035] Among them, the base 11 is preferably circular, and a non-slip rubber pad can be installed on the bottom. The bottom ring sleeve 12 and the floating ring sleeve 13 are preferably circular rings. The central pillar 14 is fixedly installed above the base 11. The floating ring sleeve 13 can be sleeved on the outside of the central pillar 14 and slide up and down to achieve height adjustment of the floating ring sleeve 13.
[0036] The mixing unit 2 includes a floating clamp 21, a rebound cap 22 and a mixing rod 23. The floating clamp 21 is movably arranged in the central pillar 14 and fixed to the floating ring sleeve 13. The rebound cap 22 is movably arranged on the top of the central pillar 14. The mixing rod 23 is provided in a plurality of groups and is circumferentially fixed outside the rebound cap 22. The top of the base 11 is circumferentially fixed with a plurality of groups of bottom brackets 111.
[0037] The floating clamp 21 is inserted into the central pillar 14 , and the floating ring 13 is connected and fixed to the central pillar 14 via the floating clamp 21 . The height of the floating ring 13 can be changed by changing the horizontal height of the floating clamp 21 .
[0038] Furthermore, the rebound cap 22 is slidably sleeved on the top of the central pillar 14. By pressing the rebound cap 22, the mixing rod 23 is controlled to float up and down. The floating mixing rod 23 can fully stir the cell mixed solution.
[0039] The outer wall of the bottom ring sleeve 12 is equidistantly fixedly connected with a plurality of groups of horizontal plates 121, and the bottom of the horizontal plates 121 is vertically fixedly connected with vertical plates 122. The vertical plates 122 are fixedly connected to the base 11 through connecting ears 123, wherein the connecting ears 123 are fixed to the base 11 by bolts.
[0040] A plurality of first fixing holes 124 are formed in the bottom ring 12 . The first fixing holes 124 are arranged circumferentially. Test tubes A are inserted into the first fixing holes 124 . The bottom of the test tube A is placed on the bottom bracket 111 .
[0041] During use, test tube A is inserted into the corresponding first fixing hole 124, and the bottom wall of test tube A is placed on the base 111. The base 111 is preferably made of rubber material, and the top has an arc-shaped groove that matches the arc-shaped bottom wall of the test tube, ensuring that test tube A can be stably placed on the base 111.
[0042] A plurality of groups of second fixing holes 131 are formed in the floating ring 13 . The second fixing holes 131 are aligned with the first fixing holes 124 . The test tube A can be inserted into the second fixing holes 131 .
[0043] During use, the test tube A is first inserted into the second fixing hole 131 from above the floating ring 13 , and then further inserted into the first fixing hole 124 , and finally placed on the base 111 .
[0044] The inner wall of the floating ring 13 is symmetrically fixed with a docking plate 132 , the end of the docking plate 132 is fixedly connected with a connecting cover shell 133 , the connecting cover shell 133 is slidably sleeved outside the central pillar 14 , and the top of the connecting cover shell 133 is fixedly connected with a top plate 1331 .
[0045] Example 2
[0046] Reference Figures 1 to 10 , which is the second embodiment of the present invention. This embodiment is different from the first embodiment in that: a rectangular through hole 141 is opened in the central pillar 14, and both ends of the rectangular through hole 141 are connected to the outside through fan-shaped through holes 142; a plurality of group card slots 1411 are opened longitudinally and equidistantly on the inner walls on both sides opposite to the rectangular through hole 141.
[0047] A sliding column 143 is fixedly connected to the top of the central pillar 14 , a sliding slot 1431 is symmetrically opened on the outer wall of the sliding column 143 , and an anti-dropping cap 1432 is fixedly connected to the top of the sliding column 143 .
[0048] The outer diameter of the sliding column 143 is smaller than the central support column 14, and the anti-drop cap 1432 is detachably fixed to the top of the sliding column 143 by bolts.
[0049] The floating clamp 21 includes a first fan-shaped block 211, a rectangular block 212 and a second fan-shaped block 213. One end of the rectangular block 212 is fixedly connected to the second fan-shaped block 213, and the other end is detachably fixed to the first fan-shaped block 211. The second fan-shaped block 213 can be integrated with the rectangular block 212. The first fan-shaped block 211 is detachably fixed to the other end of the rectangular block 212 by bolts. The outer walls of the first fan-shaped block 211 and the second fan-shaped block 213 are detachably fixedly connected to the connecting cover shell 133.
[0050] The outer walls of the first sector block 211 and the second sector block 213 are connected and fixed with arc-shaped mounting side plates, and the connecting cover shell 133 is detachably fixed to the arc-shaped mounting side plates by bolts.
[0051] The first sector block 211 and the second sector block 213 slide in the sector through holes 142 on both sides respectively.
[0052] A rectangular groove 2121 is provided on the top of the rectangular block 212, a rebound groove 2111 is provided on one end of the first sector block 211 close to the rectangular block 212, a sliding slot 2131 is provided in the middle of the second sector block 213, a clamping piece 214 is symmetrically arranged in the center of the rectangular groove 2121, the rectangular groove 2121 is connected to the rebound groove 2111, and an unlocking piece 215 is slidably inserted in the sliding slot 2131.
[0053] A top shell 216 is detachably fixed and sealed at the top opening of the rectangular block 212 . The top shell 216 is installed on the top of the rectangular block 212 by bolts. A rotating shaft 2161 is rotatably connected to the middle of the bottom end of the top shell 216 . A gear 2162 is fixedly connected to the outside of the rotating shaft 2161 .
[0054] The clamping member 214 includes a vertical plate 2141, an anti-bias slider 2142, an insertion block 2143 and a docking tooth plate 2144. The insertion block 2143 is fixedly connected to the middle of one side of the vertical plate 2141, and the docking tooth plate 2144 is fixedly connected to the other side of the vertical plate 2141. The docking tooth plates 2144 in the clamping members 214 on both sides are simultaneously meshed and connected with the gear 2162 in the middle.
[0055] The outer wall of the rectangular block 212 is symmetrically provided with external holes 2122, the card block 2143 slides through the external holes 2122, and is inserted into the card slot 1411, the anti-bias slider 2142 is fixedly connected to the bottom of the vertical plate 2141, and the anti-bias slider 2142 is slidably placed in the rectangular groove 2121.
[0056] During use, when the gear 2162 rotates, the mating tooth plates 2144 meshing therewith move to both sides, and the card insertion block 2143 extends from the external hole 2122 and is inserted into the card slot 1411, so that the overall height of the floating card member 21 can be determined.
[0057] The unlocking member 215 includes a sliding block 2151 , a pressing plate 2152 , a clamping plate 2153 and a rebound plate 2154 . The sliding block 2151 is slidably inserted into the sliding slot 2131 .
[0058] The pressing plate 2152 is fixedly connected to one end of the sliding block 2151 , and the clamping plate 2153 is symmetrically fixed to the other end of the sliding block 2151 . A plurality of racks 21531 are connected to the inner wall of one side of the clamping plate 2153 , and the racks 21531 are meshedly connected with the gear 2162 .
[0059] The rebound plate 2154 is fixedly connected to the end of the clamping plate 2153 , and the rebound plate 2154 can be inserted into the rebound groove 2111 in a sliding manner. A first spring T1 is fixedly connected between the rebound plate 2154 and the inner wall of the rebound groove 2111 .
[0060] During use, under the action of the first spring T1, the first spring T1 slightly pushes the sliding block 2151 outward through the rebound plate 2154 and the clamping plate 2153. At this time, the rack 21531 can maintain the state of the rotating shaft 2161 and the gear 2162. At this time, the insertion block 2143 extends outward and is engaged in the slot 1411.
[0061] Furthermore, if the overall height of the floating clamp 21 needs to be changed, the pressing plate 2152 is pushed inward, the sliding block 2151 moves toward the rebound groove 2111, and the rebound plate 2154 compresses the first spring T1. During the movement of the sliding block 2151, the rack 21531 connected to the side clamp 2153 can drive the rotating shaft 2161 to move. At this time, the gear 2162 on the rotating shaft 2161 can drive the docking gear plate 2144 to move, so that the clamp 214 retracts as a whole.
[0062] Furthermore, the card blocks 2143 on the synchronously retracted clamping members 214 on both sides are pulled out of the card slot 1411, and the height of the floating clamping member 21 can be manually adjusted. When the floating clamping member 21 reaches the desired height, the pressing plate 2152 is released, and the first spring T1 rebounds and resets. The card blocks 2143 pop out of the external hole 2122 again and are plugged into the card slot 1411 to fix the floating clamping member 21. At this time, the floating ring sleeve 13 connected to the floating clamping member 21 is fixed in position.
[0063] The rebound cap 22 includes a pressing cap 221 and a rebound sleeve 222 coaxially fixedly connected to the bottom thereof.
[0064] A plurality of groups of cross bars 2211 are fixedly connected to the outer wall of the bottom of the pressing cap 221 at equal intervals, a snap ring 2212 is fixedly connected to the end of the cross bar 2211, a mounting hole 22121 is provided in the middle of the snap ring 2212, pushing grooves 22122 are also provided on both sides of the mounting hole 22121, a buckle 22123 is slidably connected in the pushing groove 22122, and a second spring T2 is fixedly connected between the buckle 22123 and the inner wall of the pushing groove 22122.
[0065] Reference Figure 8 Among them, a horizontal block is connected to the buckle 22123, and the horizontal block extends into the mounting hole 22121. An L-shaped plug-in block is also fixed to the outer edge of the buckle 22123. The horizontal end of the L-shaped plug-in block can be inserted into the outer wall of the snap ring 2212. The staff can push the top of the snap ring 2212 to squeeze the second spring T2. At this time, the horizontal block shrinks from the mounting hole 22121 to the push slot 22122.
[0066] The rebound sleeve 222 includes a sleeve clamping ring 2221, the top and bottom ends of which are coaxially fixedly connected with an insert tube 2222 and a rebound ring 2223, the inner wall of the sleeve clamping ring 2221 is symmetrically fixedly connected with a clamping strip 22211, the sleeve clamping ring 2221 is slidably sleeved on the sliding column 143, and the clamping strip 22211 is slidably inserted into the sliding groove 1431.
[0067] During use, the anti-drop cap 1432 needs to be removed first, and after the clamping strip 22211 is aligned with the sliding slot 1431, the rebound sleeve 222 can be slidably inserted into the outside of the sliding column 143. Under the action of the clamping strip 22211, the rebound sleeve 222 can move up and down outside the sliding column 143.
[0068] A third spring T3 is also fixedly connected between the rebound ring 2223 and the top plate 1331 , and the third spring T3 is sleeved outside the central pillar 14 .
[0069] During use, the third spring T3 always pushes the rebound cap 22 upward. When the staff presses the pressing cap 221 downward, the third spring T3 is compressed, and the cross bar 2211 moves downward simultaneously with the pressing cap 221. Further, when the staff releases their hand, the third spring T3 resets and pushes the rebound cap 22 upward again.
[0070] The mixing rod 23 includes a connecting rod 231 and a rotating rod 232 . The outer wall of the connecting rod 231 is symmetrically provided with height adjustment grooves 2311 . The connecting rod 231 is slidably inserted into the mounting hole 22121 , and the buckle 22123 is slidably inserted into the height adjustment groove 2311 .
[0071] During use, when it is necessary to install or adjust the downward extension length of the mixing rod 23, it is only necessary to turn the buckle 22123, and the cross block retracts into the slot 22122. At this time, after changing the downward extension height of the connecting rod 231, the buckle 22123 is released again. At this time, the cross block is inserted into the corresponding height adjustment slot 2311. Under the fixation of the buckle 22123, the mixing rod 23 is fixed in the clamping ring 2212.
[0072] The bottom end of the connecting rod 231 is also connected to a rotating drum 233 , the top end of the rotating drum 233 is rotatably sleeved on the connecting rod 231 , and the top end of the rotating rod 232 is fixedly inserted in the middle of the bottom end of the rotating drum 233 .
[0073] The outer wall of the bottom end of the rotating rod 232 is connected to a plurality of groups of stirring blades 2321, and the edges of the stirring blades 2321 are fixedly connected to a converging tube 2322, which is rotatably inserted into the test tube A.
[0074] During use, when the connecting rod 231 moves downward, the rotating rod 232 moves downward with the connecting rod 231. Under the action of the cell mixture in the test tube A, the cell mixture will push the stirring blades 2321. The flowing cell mixture drives the stirring blades 2321 to rotate. The focusing tube 2322 can accelerate the flow rate of the cell mixture on the stirring blades 2321 to ensure the smooth rotation of the rotating rod 232.
[0075] The remaining structures are the same as those of Example 1.
[0076] Example 3
[0077] Reference Figures 1 to 10 , which is the third embodiment of the present invention, is different from the second embodiment in that it provides a method for using a mixing device for cell experiments, which is based on the mixing device for cell experiments in the above embodiment and includes the following steps:
[0078] First, inject various cell solutions required to be mixed into the test tube A, and then put the test tube A into the bottom ring sleeve 12 and the floating ring sleeve 13.
[0079] The height of the floating ring 13 is adjusted to ensure that the floating ring 13 is always below the top opening of the test tube A to prevent the test tube A from tipping over.
[0080] During the adjustment process, the pressing plate 2152 is pushed inward, and the sliding block 2151 moves toward the rebound groove 2111. The rebound plate 2154 compresses the first spring T1. During the movement of the sliding block 2151, the rack 21531 connected to the side clamp 2153 can drive the rotating shaft 2161 to move. At this time, the gear 2162 on the rotating shaft 2161 can drive the docking gear plate 2144 to move, so that the clamping member 214 retracts as a whole.
[0081] Furthermore, the card blocks 2143 on the synchronously retracted clamping members 214 on both sides are pulled out of the card slot 1411, and the height of the floating clamping member 21 can be manually adjusted. When the floating clamping member 21 reaches the desired height, the pressing plate 2152 is released, and the first spring T1 rebounds and resets. The card blocks 2143 pop out of the external hole 2122 again and are plugged into the card slot 1411 to fix the floating clamping member 21. At this time, the floating ring sleeve 13 connected to the floating clamping member 21 is fixed in position.
[0082] The height of the mixing rod 23 is adjusted to ensure that the stirring part at the bottom of the mixing rod 23 extends into the cell mixture solution.
[0083] When the test tube is short, the height of the mixing rod 23 needs to be adjusted downward, and when the test tube is long, the height of the mixing rod 23 needs to be adjusted upward, and it is necessary to ensure that the rotating drum 233 is always above the liquid surface of the cell mixture.
[0084] Press the rebound cap 22 with your finger, and the height of the rebound cap 22 will be reduced, driving the mixing rod 23 to descend and stir the cell mixed solution. When the mixing rod 23 descends, it will be rotated by the cell mixed solution and rotate synchronously in the circumferential direction, thereby improving the cell mixing efficiency.
[0085] The remaining structure is the same as that of Example 2.
[0086] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the spirit and scope of the technical solutions of the present invention, which should all be included in the scope of the claims of the present invention.
Claims
1. A mixing device for cell experiments, characterized in that: include, The support unit (1) comprises a base (11), a bottom ring sleeve (12), a floating ring sleeve (13) and a central pillar (14), wherein the central pillar (14) is fixedly arranged in the middle of the base (11), the bottom ring sleeve (12) is fixedly connected to the top of the base (11), and the floating ring sleeve (13) is movably connected to the central pillar (14); The mixing unit (2) comprises a floating clamp (21), a rebound cap (22) and a mixing rod (23), wherein the floating clamp (21) is movably arranged in a central pillar (14) and fixed to the floating ring sleeve (13), the rebound cap (22) is movably arranged on the top of the central pillar (14), and the mixing rod (23) is provided in a plurality of groups and is circumferentially fixed outside the rebound cap (22).
2. The mixing device for cell experiments according to claim 1, characterized in that: A plurality of groups of bottom brackets (111) are fixedly connected in a circumferential direction to the top of the base (11); The outer wall of the bottom ring sleeve (12) is equidistantly and fixedly connected with a plurality of groups of horizontal plates (121); the bottom of the horizontal plates (121) is vertically and fixedly connected with vertical plates (122); and the vertical plates (122) are fixedly connected to the base (11) via connecting ears (123); The bottom ring sleeve (12) is also provided with a plurality of groups of first fixing holes (124), the first fixing holes (124) are arranged in a circumferential direction, and a test tube (A) is inserted into the first fixing holes (124), and the bottom of the test tube (A) is placed on the bottom bracket (111).
3. The mixing device for cell experiments according to claim 2, characterized in that: The floating ring (13) is provided with a plurality of groups of second fixing holes (131), the second fixing holes (131) are aligned with the first fixing holes (124), and the test tube (A) can be inserted into the second fixing holes (131); A docking plate (132) is symmetrically fixed on the inner wall of the floating ring sleeve (13), and a connecting cover shell (133) is fixedly connected to the end of the docking plate (132). The connecting cover shell (133) is slidably sleeved outside the central pillar (14), and a top plate (1331) is fixedly connected to the top of the connecting cover shell (133).
4. The mixing device for cell experiments according to claim 3, characterized in that: A rectangular through hole (141) is provided in the central pillar (14), and two ends of the rectangular through hole (141) are connected to the outside through fan-shaped through holes (142); The inner walls of the two opposite sides of the rectangular through hole (141) are longitudinally and equidistantly provided with a plurality of slots (1411); A sliding column (143) is fixedly connected to the top of the central pillar (14), a sliding groove (1431) is symmetrically provided on the outer wall of the sliding column (143), and an anti-drop cap (1432) is fixedly connected to the top of the sliding column (143).
5. The mixing device for cell experiments according to claim 4, characterized in that: The floating clamp (21) comprises a first sector block (211), a rectangular block (212) and a second sector block (213); one end of the rectangular block (212) is fixedly connected to the second sector block (213), and the other end is detachably fixed to the first sector block (211); the outer walls of the first sector block (211) and the second sector block (213) are detachably fixedly connected to the connection cover shell (133); The first sector block (211) and the second sector block (213) slide in the sector through holes (142) on both sides respectively; A rectangular groove (2121) is provided on the top of the rectangular block (212); a rebound groove (2111) is provided on one end of the first sector-shaped block (211) close to the rectangular block (212); a sliding slot (2131) is provided in the middle of the second sector-shaped block (213); a clamping piece (214) is symmetrically arranged in the center of the rectangular groove (2121); and an unlocking piece (215) is slidably inserted in the sliding slot (2131).
6. The mixing device for cell experiments according to claim 5, characterized in that: A top shell (216) is sealed and detachably fixed at the top opening of the rectangular block (212); a rotating shaft (2161) is rotatably connected to the middle of the bottom end of the top shell (216); and a gear (2162) is fixedly connected to the outside of the rotating shaft (2161); The clamping member (214) comprises a vertical plate (2141), an anti-bias slider (2142), a card insertion block (2143) and a docking tooth plate (2144); the card insertion block (2143) is fixedly connected to the middle of one side of the vertical plate (2141); the docking tooth plate (2144) is fixedly connected to the other side of the vertical plate (2141); and the docking tooth plates (2144) in the clamping members (214) on both sides are simultaneously meshed and connected with the gear (2162) in the middle. The outer wall of the rectangular block (212) is symmetrically provided with external holes (2122); the card insertion block (2143) slides through the external holes (2122) and is inserted into the card slot (1411); the anti-deflection slider (2142) is fixedly connected to the bottom of the vertical plate (2141), and the anti-deflection slider (2142) is placed in the rectangular groove (2121) in a sliding manner.
7. The mixing device for cell experiments according to claim 6, characterized in that: The unlocking member (215) comprises a sliding block (2151), a pressing plate (2152), a clamping plate (2153) and a rebound plate (2154); the sliding block (2151) is slidably inserted into the sliding slot (2131); The pressing plate (2152) is fixedly connected to one end of the sliding block (2151), the clamping plate (2153) is symmetrically fixed to the other end of the sliding block (2151), and the inner wall of one side of the clamping plate (2153) is connected to a plurality of groups of racks (21531), and the racks (21531) are meshedly connected with the gears (2162); The rebound plate (2154) is fixedly connected to the end of the clamping plate (2153), and the rebound plate (2154) can be inserted into the rebound groove (2111) by sliding, and a first spring (T1) is fixedly connected between the rebound plate (2154) and the inner wall of the rebound groove (2111).
8. The mixing device for cell experiments according to any one of claims 4 to 7, characterized in that: The resilient cap (22) comprises a pressing cap (221) and a resilient sleeve (222) coaxially fixedly connected to the bottom thereof; A plurality of groups of cross bars (2211) are fixedly connected to the outer wall of the bottom of the pressing cap (221) at equal intervals, a snap ring (2212) is fixedly connected to the end of the cross bar (2211), a mounting hole (22121) is provided in the middle of the snap ring (2212), and push grooves (22122) are provided on both sides of the mounting hole (22121), a buckle (22123) is slidably connected in the push groove (22122), and a second spring (T2) is fixedly connected between the buckle (22123) and the inner wall of the push groove (22122); The rebound sleeve (222) comprises a sleeve clamping ring (2221), the top and bottom ends of the sleeve clamping ring (2221) are coaxially fixedly connected with an inserting tube (2222) and a rebound ring (2223), the inner wall of the sleeve clamping ring (2221) is symmetrically fixedly connected with a clamping strip (22211), the sleeve clamping ring (2221) is slidably sleeved on the sliding column (143), and the clamping strip (22211) is slidably inserted into the sliding clamping groove (1431); A third spring (T3) is also fixedly connected between the rebound ring (2223) and the top plate (1331), and the third spring (T3) is sleeved outside the central pillar (14).
9. The mixing device for cell experiments according to claim 1, characterized in that: The mixing rod (23) comprises a connecting rod (231) and a rotating rod (232); the outer wall of the connecting rod (231) is symmetrically provided with height adjustment grooves (2311); the connecting rod (231) is slidably inserted into the mounting hole (22121), and the buckle (22123) is slidably inserted into the height adjustment groove (2311); The bottom end of the connecting rod (231) is also connected to a rotating drum (233), the top end of the rotating drum (233) is rotatably sleeved on the connecting rod (231), and the top end of the rotating rod (232) is fixedly inserted in the middle of the bottom end of the rotating drum (233); The outer wall of the bottom end of the rotating rod (232) is connected to a plurality of groups of stirring blades (2321), and the edges of the stirring blades (2321) are fixedly connected to a converging tube (2322), which is rotatably inserted into the test tube (A).
10. A method for using a mixing device for cell experiments, characterized in that: The mixing device for cell experiments according to any one of claims 2 to 9 comprises the following steps: First, inject various cell solutions to be mixed into the test tube (A), and place the test tube (A) into the bottom ring (12) and the floating ring (13); Adjust the height of the floating ring (13) to ensure that the floating ring (13) is always below the top opening of the test tube (A) to prevent the test tube (A) from tipping over; Adjust the height of the mixing rod (23) to ensure that the stirring part at the bottom of the mixing rod (23) extends into the cell mixture solution; The rebound cap (22) is pressed with a finger, and the height of the rebound cap (22) is lowered, driving the mixing rod (23) to descend to stir the cell mixed solution. When the mixing rod (23) descends, it is rotated by the cell mixed solution and rotates synchronously in the circumferential direction, thereby improving the cell mixing efficiency.