Cell storage device and cell storage method for optimizing cell preservation

Abstract

The cell storage device comprises a storage box, the top of the storage box is provided with a material taking and placing port used for taking and placing test tubes into the storage box, the two ends of the interior of the storage box are symmetrically and fixedly connected with a first vertical frame and a second vertical frame, and lifting grooves are formed in the first vertical frame and the second vertical frame; lifting plates are slidably arranged in the lifting grooves, a supporting shaft is fixedly connected between the two lifting plates, cross-shaped plates are fixedly connected to the two ends of the supporting shaft, and cell storage mechanisms are rotatably connected between the corresponding ends of the two cross-shaped plates through bearings; the device has the beneficial effects that through the synergistic effect of the rotary cell storage mechanism and the liquid nitrogen freezing system, cell samples in all test tubes are uniformly exposed in a low-temperature environment, cell damage caused by non-uniform local temperature is avoided, and when cells are taken and placed, the test tube mounting rack can be controlled to be lifted to the position of a material taking and placing opening, so that the test tubes are conveniently taken and placed. The target sample can be accurately stored and taken without integrally taking out the storage rack.

Description

Technical Field

[0001] The present invention relates to the technical field of cell storage devices, and in particular to a cell storage device and a cell storage method for optimizing cell preservation. Background Art

[0002] Cell cryopreservation is one of the main methods of cell preservation. Using cryopreservation technology to store cells in liquid nitrogen at -196℃ can temporarily remove cells from the growth state and preserve their cell characteristics, so that the cells can be revived for experiments when needed. Moreover, moderate preservation of a certain amount of cells can prevent cell loss due to contamination of cells being cultured or other unexpected events, thus playing a role in cell preservation. In addition, cell cryopreservation can also be used to purchase, donate, exchange and transport certain cells. When cells are cryopreserved, adding a protective agent to the culture medium - a final concentration of 5%-15% glycerol or dimethyl sulfoxide can lower the freezing point of the solution. In addition, under slow freezing conditions, water in the cells leaks out, reducing the formation of ice crystals, thereby avoiding cell damage. The general storage container is a cell cryopreservation tube.

[0003] Cell preservation technology plays a vital role in biomedical research, clinical treatment and biobank construction. The quality of cell preservation directly affects the activity and function of cells and the reliability of subsequent experiments or treatments. At present, cell preservation mainly relies on cryogenic freezing technology, especially liquid nitrogen freezing, which can preserve cells in an extremely low temperature environment (usually -196°C) for a long time to maximize the activity and stability of cells.

[0004] Traditional cell storage devices usually adopt a static storage method, fixing the cell test tubes in a storage rack and storing them in a freezer. When accessing a specific sample, the entire storage rack needs to be taken out of the freezer, which is not only cumbersome to operate, but may also cause temperature fluctuations of other samples, affecting the cell preservation effect. Static storage may cause uneven temperature distribution in the storage box, and the temperature in some areas may be higher than the ideal preservation temperature, thereby affecting the quality of cell preservation. To this end, we propose a cell storage device and a cell storage method for optimizing cell preservation. Summary of the invention

[0005] The object of the present invention is to provide a cell storage device and a cell storage method for optimizing cell preservation, so as to solve the problems raised in the above-mentioned background technology.

[0006] To achieve the above object, the present invention provides the following technical solution: A cell storage device for optimizing cell preservation, including a storage box, a loading and unloading opening for taking and placing test tubes into and out of the storage box is provided at the top of the storage box, first and second vertical frames are symmetrically and fixedly connected at both ends inside the storage box, a lifting groove is formed in the first and second vertical frames, and a lifting plate is slidably arranged in the lifting groove. A support shaft is fixedly connected between the two lifting plates, cross-shaped plates are fixedly connected to both ends of the support shaft, and cell storage mechanisms are rotatably connected between the corresponding ends of the two cross-shaped plates through bearings. A driving motor is fixedly installed on the side wall of the lifting plate in the first vertical frame, and the end of the output shaft of the driving motor passes through the lifting plate and is fixedly connected to the support shaft. A liquid nitrogen freezing mechanism for freezing cells is arranged in the storage box.

[0007] Preferably, the cell storage mechanism includes a test tube mounting rack. There are four test tube mounting racks, and the overall structure of the four test tube mounting racks is a frame structure. Both ends of the test tube mounting rack are rotatably connected between the corresponding ends of the two cross-shaped plates through bearings. Slots are equidistantly formed at the bottom of the inner wall of the test tube mounting rack, positioning grooves are equidistantly formed at the top of the test tube mounting rack, and the positioning grooves are located directly above the slots. Test tubes for storing cells are clamped in the slots and positioning grooves.

[0008] Preferably, a fixed disk is fixedly connected to one end of the storage box. A connecting shaft is rotatably connected to the side wall of the lifting plate in the second vertical frame, and one end of the connecting shaft passes through the lifting plate and is fixedly connected to the support shaft. The other end of the connecting shaft passes through the storage box and the fixed disk, and indicating rods are equidistantly fixedly connected to the end of the connecting shaft outside the fixed disk. The four indicating rods are correspondingly arranged with the cross-shaped plates, and labels for marking cell types are arranged at the ends of the indicating rods.

[0009] Preferably, the liquid nitrogen freezing mechanism includes a liquid nitrogen tank, a ventilation pipe, a branch pipe, and a cold air outlet. The liquid nitrogen tank is installed at the bottom of the inner wall of the storage box, and a ventilation pipe is fixedly connected to the outlet end of the liquid nitrogen tank. The ventilation pipe is installed at the top end inside the storage box in a matching manner. Branch pipes are equidistantly arranged on the outer side of the ventilation pipe, and the branch pipes are fixedly installed on the inner wall of the storage box. Cold air outlets are equidistantly arranged on the outer side of the branch pipes, and the cold air outlets are arranged towards the central part inside the storage box. A control valve is installed on the outer side of the ventilation pipe at one end of the liquid nitrogen tank.

[0010] Preferably, lifting handles are symmetrically arranged at both ends of the storage box.

[0011] Preferably, electric telescopic rods are installed at the bottom ends inside the first vertical frame and the second vertical frame, and the end of the piston rod of the electric telescopic rod extends into the lifting groove and is fixedly connected to the lifting plate. Limiting grooves are provided on the corresponding two side walls of the first vertical frame and the second vertical frame, and limiting blocks are integrally provided on the corresponding two side walls of the lifting plate, and the limiting blocks are slidably connected to the inside of the limiting grooves in a matching manner.

[0012] Preferably, observation windows are provided on the corresponding two side walls of the storage box, and support pads are provided at the four corners of the bottom of the storage box.

[0013] Preferably, counterweight pieces are provided at the bottom of the test tube mounting rack.

[0014] Preferably, a connection cover is connected to the top of the loading and unloading opening in a matching manner, and a control handle is provided on the top of the connection cover.

[0015] A cell storage method for a cell storage device for optimizing cell preservation according to any one of the above includes the following steps:

[0016] Step 1: Open the connection cover, control the electric telescopic rod to work, drive the test tube mounting rack at the uppermost end of the lifting plate to rise into the loading and unloading opening, and quickly place the cell cryopreservation tubes containing cells on the test tube mounting rack one by one;

[0017] Step 2: Drive the two lifting plates to return to their original heights through the electric telescopic rod. At this time, the drive motor works to drive the cross-shaped plate to rotate by 90 degrees, and then cooperate with the electric telescopic rod to work, continue to place the empty test tube mounting rack at the loading and unloading opening, place the cell cryopreservation tubes containing another type of cell again, and then control the lifting plate to return to its original height again. The drive motor controls the cross-shaped plate to continue to rotate by 90 degrees, so as to store the cell cryopreservation tubes on all the test tube mounting racks on the cell storage mechanism in sequence;

[0018] Step 3: After all the placements are completed, the cell storage mechanism returns to its original height, cover the connection cover, and the liquid nitrogen tank releases cold air into the storage box. At the same time, the drive motor works to drive the cross-shaped plate to rotate, so that the positions of different test tube mounting racks change in the storage box, enabling the cells to fully contact the cold air. During the rotation of the cross-shaped plate, the indicating rod also rotates accordingly, and the cell type is marked at the label of the indicating rod;

[0019] Step 4: When retrieving cells, the drive motor can be controlled to rotate so that the label of the cells to be retrieved is located at the top. Open the connection cover and control the electric telescopic rod to work, so that the lifting plate rises to the set height position. At this time, the top of the cell cryopreservation tube is located outside the loading and unloading opening, which is convenient for personnel to retrieve. The cell cryopreservation tubes storing other types of cells are located inside the storage box and continue to be cryopreserved. The opening of the loading and unloading opening is small, and the test tube mounting rack blocks the position of the loading and unloading opening after rising, avoiding the leakage of cold air inside the storage box.

[0020] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0021] 1. Through the synergistic effect of the rotary cell storage mechanism and the liquid nitrogen freezing system, the cell samples in all test tubes are evenly exposed to a low-temperature environment, avoiding cell damage caused by uneven local temperature and ensuring the stability of long-term storage.

[0022] 2. The electric telescopic rod of the present invention can adjust the height of the lifting plate, enabling the test tube mounting rack to be lifted to the position of the loading and unloading port, so that the target sample can be accurately accessed without removing the entire storage rack, reducing temperature fluctuations and operation time.

[0023] 3. Different sample categories are marked by the indicating rods and labels on the fixed disk. Combined with the rotary storage rack, the target test tube can be quickly located, avoiding confusion or errors caused by manual searching.

[0024] 4. The liquid nitrogen freezing system of the present invention evenly distributes cold air through the branch pipes and cold air outlets. Cooperating with the rotary storage mechanism, the cold air circulates efficiently, reducing liquid nitrogen waste and maintaining a more stable low-temperature environment. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 is a schematic structural diagram of the overall structure of the present invention;

[0026] Figure 2 is a schematic structural diagram of the internal connection structure of the present invention;

[0027] Figure 3 is a schematic structural diagram of the connection structure of the fixed disk of the present invention;

[0028] Figure 4 is a schematic structural diagram of the test tube mounting rack of the present invention;

[0029] Figure 5 is a schematic structural diagram of the cell storage mechanism of the present invention;

[0030] Figure 6 is a schematic structural diagram of the liquid nitrogen freezing mechanism of the present invention;

[0031] Figure 7 is a schematic structural diagram of the cell storage mechanism after being raised of the present invention.

[0032] In the figure: 1, storage box; 2, loading and unloading port; 3, connecting cover; 4, first vertical frame; 5, second vertical frame; 6, lifting plate; 7, support shaft; 8, cross-shaped plate; 9, test tube mounting rack; 10, counterweight piece; 11, card slot; 12, positioning slot; 13, cell storage mechanism; 14, liquid nitrogen freezing mechanism; 15, drive motor; 16, electric telescopic rod; 17, limit slot; 18, connecting shaft; 19, fixed disk; 20, indicating rod; 21, label; 22, liquid nitrogen tank; 23, ventilation pipe; 24, control valve; 25, branch pipe; 26, cold air outlet; 27, lifting handle; 28, control handle; 29, observation window; 30, support pad. Detailed implementation manner

[0033] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0034] Please refer to Figure 1 , Figure 2 , Figure 3 and Figure 7 , the present invention provides a technical solution: a cell storage device for optimizing cell preservation, including a storage box 1. A loading and unloading port 2 for loading and unloading test tubes into and out of the storage box 1 is provided at the top of the storage box 1. First vertical frames 4 and second vertical frames 5 are symmetrically and fixedly connected to both ends inside the storage box 1. Lifting grooves are formed in the first vertical frame 4 and the second vertical frame 5, and a lifting plate 6 is slidably arranged in the lifting grooves. A support shaft 7 is fixedly connected between the two lifting plates 6. Cross-shaped plates 8 are fixedly connected to both ends of the support shaft 7. A cell storage mechanism 13 is rotatably connected between the corresponding ends of the two cross-shaped plates 8 through bearings.

[0035] It should be noted that a detachable maintenance plate is provided on the longer side wall of the storage box 1. The maintenance plate is detachably connected to the storage box 1 by screws, and a sealing ring is provided at the connection between the maintenance plate and the storage box 1, which is convenient for maintaining the components inside the storage box 1.

[0036] A drive motor 15 is fixedly installed on the side wall of the lifting plate 6 inside the first vertical frame 4, and the end of the output shaft of the drive motor 15 passes through the lifting plate 6 and is fixedly connected to the support shaft 7. A liquid nitrogen freezing mechanism 14 for freezing cells is provided inside the storage box 1.

[0037] It should be noted that the device is provided with a controller. By controlling the operation of the driving motor 15, the rotation of the support shaft 7 can be controlled. During the rotation of the support shaft 7, the two cross-shaped plates 8 are driven to rotate, thereby driving the four cell storage mechanisms 13 to rotate. The synergistic effect of the rotary cell storage mechanism 13 and the liquid nitrogen freezing system can evenly expose the cell samples in all test tubes to a low-temperature environment.

[0038] Please refer to Figure 4 and Figure 5 , the cell storage mechanism 13 includes a test tube mounting rack 9. There are four test tube mounting racks 9, and the overall structure of the four test tube mounting racks 9 is a frame structure. Both ends of the test tube mounting rack 9 are rotatably connected between the corresponding ends of the two cross-shaped plates 8 through bearings. At equal intervals, card slots 11 are opened at the bottom of the inner wall of the test tube mounting rack 9, and positioning slots 12 are opened at equal intervals at the top of the test tube mounting rack 9, and the positioning slots 12 are located directly above the card slots 11. Test tubes for storing cells are clamped in the card slots 11 and the positioning slots 12.

[0039] It should be noted that there are four test tube mounting racks 9, and the four test tube mounting racks 9 are respectively rotatably connected between the four ends of the two cross-shaped plates 8 through bearings. When the two cross-shaped plates 8 rotate, the test tube mounting rack 9 rotates accordingly. Under the action of the gravity of the test tube mounting rack 9, the test tube mounting rack 9 itself always remains in a vertical state, which can prevent the cell cryopreservation tubes storing cells from tipping over.

[0040] Please refer to Figure 1 and Figure 3 , a fixed disk 19 is fixedly connected to one end of the storage box 1. A connecting shaft 18 is rotatably connected to the side wall of the lifting plate 6 in the second vertical frame 5, and one end of the connecting shaft 18 passes through the lifting plate 6 and is fixedly connected to the support shaft 7. The other end of the connecting shaft 18 passes through the storage box 1 and the fixed disk 19, and equally spaced indicating rods 20 are fixedly connected to the end of the connecting shaft 18 located outside the fixed disk 19. The four indicating rods 20 are correspondingly arranged with the cross-shaped plates 8. Labels 21 for marking cell types are provided at the ends of the indicating rods 20.

[0041] It should be noted that when the driving motor 15 drives the support shaft 7 to rotate, the support shaft 7 drives the connecting shaft 18 to rotate. The rotation of the connecting shaft 18 drives the four indicating rods 20 to rotate, so that the four indicating rods 20 respectively correspond to the four test tube mounting racks 9. The types of test tubes on each test tube mounting rack 9 are marked on the labels 21 on the corresponding indicating rods 20, which is convenient for personnel to know the location of each type of cryopreserved cell.

[0042] Please refer to Figure 2 and Figure 6, the liquid nitrogen freezing mechanism 14 includes a liquid nitrogen tank 22, a ventilation pipe 23, branch pipes 25, and a cold air outlet 26. The liquid nitrogen tank 22 is installed at the bottom of the inner wall of the storage box 1, and the outlet end of the liquid nitrogen tank 22 is fixedly connected to the ventilation pipe 23. The ventilation pipe 23 is installed at the top inside the storage box 1. Branch pipes 25 are equidistantly arranged on the outer side of the ventilation pipe 23, and the branch pipes 25 are fixedly installed on the inner wall of the storage box 1. Cold air outlets 26 are equidistantly arranged on the outer side of the branch pipes 25, and the cold air outlets 26 are arranged towards the central part inside the storage box 1. A control valve 24 is installed on the outer side of one end of the ventilation pipe 23 where it is located at the liquid nitrogen tank 22.

[0043] It should be noted that when the present invention is in use, open the connection cover 3, and control the electric telescopic rod 16 to work through the controller provided by the device. The electric telescopic rod 16 controls the two lifting plates 6 to rise to a certain height, thereby driving the test tube mounting rack 9 at the uppermost end of the lifting plate 6 to rise into the loading and unloading port 2. At this time, the cryogenic vials containing cells can be quickly placed one by one into the positioning grooves 12 on the test tube mounting rack 9, so that the bottom end of the cryogenic vial is stuck into the card slot 11 of the test tube mounting rack 9. After the placement is completed, then drive the two lifting plates 6 to reset their heights through the electric telescopic rod 16. At this time, the drive motor 15 works to drive the cross-shaped plate 8 to rotate by ninety degrees, and then cooperate with the electric telescopic rod 16 to work, and continue to place the empty test tube mounting rack 9 at the loading and unloading port 2, and place the cryogenic vials containing another type of cells again. Then control the lifting plate 6 to reset its height again, and the drive motor 15 controls the cross-shaped plate 8 to continue to rotate by ninety degrees, so as to store the cryogenic vials on all the test tube mounting racks 9 on the cell storage mechanism 13 in sequence. After all the placements are completed, the cell storage mechanism 13 resets its height. The cell storage mechanism 13 is located inside the storage box 1. Cover the connection cover 3, and the liquid nitrogen tank 22 releases cold air into the storage box 1. At the same time, the drive motor 15 works to drive the cross-shaped plate 8 to rotate, so that the positions of different test tube mounting racks 9 change inside the storage box 1, enabling the cells to fully contact the cold air and achieving better cryopreservation. During the rotation of the cross-shaped plate 8, the indicating rod 20 also rotates accordingly. The label 21 on the indicating rod 20 is marked with the cell type. When retrieving cells, the drive motor 15 can be controlled to rotate so that the label 21 of the cells to be retrieved is located at the top. Open the connection cover 3 and control the electric telescopic rod 16 to work, so that the lifting plate 6 rises to the set height position. At this time, the top end of the cryogenic vial is located outside the loading and unloading port 2, which is convenient for personnel to retrieve. And the cryogenic vials storing other types of cells are located inside the storage box 1 and continue to be cryopreserved. The opening of the loading and unloading port 2 is small, and the test tube mounting rack 9 blocks the position of the loading and unloading port 2 after rising, avoiding the leakage of cold air inside the storage box 1.

[0044] Please refer to Figure 1 , lifting handles 27 are symmetrically arranged at both ends of the storage box 1.

[0045] It should be noted that the provision of two lifting handles 27 facilitates the lifting of the storage box 1 by two persons to transfer the position of the storage box 1.

[0046] Please refer to Figure 2 and Figure 3 , electric telescopic rods 16 are installed at the bottom ends inside the first vertical frame 4 and the second vertical frame 5, and the end of the piston rod of the electric telescopic rod 16 extends into the lifting groove and is fixedly connected to the lifting plate 6. Limiting grooves 17 are provided on the corresponding two side walls of the first vertical frame 4 and the second vertical frame 5, and limiting blocks are integrally provided on the corresponding two side walls of the lifting plate 6, and the limiting blocks are slidably connected inside the limiting grooves 17 in a matching manner.

[0047] It should be noted that by the operation of the electric telescopic rod 16, the lifting of the lifting plate 6 can be controlled, thereby realizing the height adjustment of the cell storage mechanism 13, and the limiting groove 17 can prevent the lifting plate 6 from shifting during the lifting and lowering process.

[0048] Please refer to Figure 1 , observation windows 29 are provided on the corresponding two side walls of the storage box 1, and support pads 30 are provided at the four corners of the bottom of the storage box 1.

[0049] It should be noted that the provision of the observation window 29 facilitates observing the internal situation, and the provision of the support pad 30 makes the overall position of the storage box 1 stable.

[0050] Please refer to Figure 4 , counterweight pieces 10 are provided at the bottom of the test tube mounting rack 9.

[0051] It should be noted that the counterweight pieces 10 can ensure rotational balance, make the overall structure of the test tube mounting rack 9 stable, reduce the risk of human operation errors or mechanical failures, and ensure the safety of samples.

[0052] Please refer to Figure 1 , a connection cover 3 is connected in a matching manner at the top of the loading and unloading port 2, and a control handle 28 is provided at the top of the connection cover 3.

[0053] It should be noted that the provision of the control handle 28 facilitates the taking of the connection cover 3 and is convenient to operate.

[0054] A cell storage method for a cell storage device for optimizing cell preservation according to any one of the above includes the following steps:

[0055] Step 1: Open the connection cover 3, control the electric telescopic rod 16 to work, drive the test tube mounting rack 9 at the uppermost end of the lifting plate 6 to rise into the loading and unloading port 2, and quickly place the cell cryopreservation tubes containing cells on the test tube mounting rack 9 one by one;

[0056] Step 2: Drive the two lifting plates 6 to reset their heights through the electric telescopic rod 16. At this time, the driving motor 15 operates to drive the cross-shaped plate 8 to rotate by 90 degrees. Then, in cooperation with the operation of the electric telescopic rod 16, continue to place the empty test tube mounting rack 9 at the loading and unloading port 2, and place the cryopreservation tube containing another type of cell again. Then, control the lifting plate 6 to reset its height again, and the driving motor 15 controls the cross-shaped plate 8 to continue rotating by 90 degrees, so as to store the cryopreservation tubes on all the test tube mounting racks 9 on the cell storage mechanism 13 in sequence;

[0057] Step 3: After all placements are completed, the cell storage mechanism 13 resets its height, cover the connection cover 3, and the liquid nitrogen tank 22 releases cold air into the storage box 1. At the same time, the driving motor 15 operates to drive the cross-shaped plate 8 to rotate, so that the positions of different test tube mounting racks 9 change within the storage box 1, enabling the cells to fully contact the cold air. During the rotation of the cross-shaped plate 8, the indicating rod 20 also rotates accordingly. The cell type is marked at the label 21 of the indicating rod 20;

[0058] Step 4: When retrieving cells, the driving motor 15 can be controlled to rotate so that the label 21 of the cells to be retrieved is located at the topmost position. Open the connection cover 3 and control the electric telescopic rod 16 to operate, so that the lifting plate 6 rises to the set height position. At this time, the top end of the cryopreservation tube is located outside the loading and unloading port 2, facilitating the retrieval by personnel. The cryopreservation tubes storing other types of cells are located inside the storage box 1 and continue to be cryopreserved. The opening of the loading and unloading port 2 is relatively small, and the test tube mounting rack 9 blocks the position of the loading and unloading port 2 after rising, avoiding the leakage of cold air inside the storage box 1.

[0059] In summary, the present invention adopts a dynamic rotation storage method to enable the cell samples to uniformly contact the low-temperature environment, greatly improving the uniformity and stability of preservation. The electric lifting mechanism is combined with a precise positioning system to make the sample access more convenient and efficient, avoiding the temperature fluctuations caused by frequent opening of the cover in the traditional method.

[0060] In addition, the terms "first", "second", "third", and "fourth" are only used for descriptive purposes and cannot be understood as indicating or implying relative importance or implicitly specifying the quantity of the indicated technical features. Thus, the features defined with "first", "second", "third", and "fourth" may explicitly or implicitly include at least one such feature.

[0061] In the present invention, unless otherwise clearly specified or limited, terms such as "installation", "setting", "connection", "fixation", "swivel connection", etc. shall be understood in a broad sense. For example, it may be a fixed connection, a detachable connection, or integrated; it may be a mechanical connection or an electrical connection; it may be directly connected or indirectly connected through an intermediate medium, and it may be the communication inside two components or the interaction relationship between two components. Unless otherwise clearly limited, for those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0062] Although the embodiments of the present invention have been shown and described, for those of ordinary skill in the art, it can be understood that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principles and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A cell storage device for optimizing cell preservation, characterized in that, It includes a storage box (1), at the top of the storage box (1) there is a loading and unloading port (2) for taking and placing test tubes into and out of the storage box (1), at both ends inside the storage box (1) there are symmetrically fixedly connected a first vertical frame (4) and a second vertical frame (5), in the first vertical frame (4) and the second vertical frame (5) there are lifting grooves opened, and in the lifting grooves there is a slidingly arranged lifting plate (6), between the two lifting plates (6) there is a fixedly connected support shaft (7), at both ends of the support shaft (7) there are fixedly connected cross-shaped plates (8), between the corresponding ends of the two cross-shaped plates (8) there is a rotatably connected cell storage mechanism (13) through bearings, on the side wall of the lifting plate (6) inside the first vertical frame (4) there is fixedly installed a driving motor (15), and the end of the output shaft of the driving motor (15) passes through the lifting plate (6) and is fixedly connected with the support shaft (7), inside the storage box (1) there is a liquid nitrogen freezing mechanism (14) for freezing cells.

2. The cell storage device for optimizing cell preservation according to claim 1, wherein: The cell storage mechanism (13) includes test tube mounting racks (9), there are four test tube mounting racks (9), and the whole of the four test tube mounting racks (9) is in a frame structure, both ends of the test tube mounting rack (9) are rotatably connected between the corresponding ends of the two cross-shaped plates (8) through bearings, at the bottom of the inner wall of the test tube mounting rack (9) there are equidistantly opened card slots (11), at the top of the test tube mounting rack (9) there are equidistantly opened positioning slots (12), and the positioning slots (12) are located directly above the card slots (11), in the card slots (11) and the positioning slots (12) there is a card-connected test tube for storing cells.

3. The cell storage device for optimizing cell preservation according to claim 1, characterized in that: One end of the storage box (1) is fixedly connected with a fixed disk (19), on the side wall of the lifting plate (6) inside the second vertical frame (5) there is a rotatably connected connecting shaft (18), and one end of the connecting shaft (18) passes through the lifting plate (6) and is fixedly connected with the support shaft (7), the other end of the connecting shaft (18) passes through the storage box (1) and the fixed disk (19), and at the end of the connecting shaft (18) outside the fixed disk (19) there are equidistantly fixedly connected indicating rods (20), and the four indicating rods (20) are correspondingly arranged with the cross-shaped plates (8), at the end of the indicating rod (20) there is a label (21) for marking the cell type.

4. The cell storage device for optimizing cell preservation according to claim 1, characterized in that: The liquid nitrogen freezing mechanism (14) includes a liquid nitrogen tank (22), a ventilation pipe (23), a branch pipe (25) and a cold air outlet (26), at the bottom of the inner wall of the storage box (1) there is installed a liquid nitrogen tank (22), and at the outlet end of the liquid nitrogen tank (22) there is a fixedly connected ventilation pipe (23), the ventilation pipe (23) is cooperatively installed at the top inside the storage box (1), on the outside of the ventilation pipe (23) there are equidistantly arranged branch pipes (25), and the branch pipes (25) are fixedly installed on the inner wall of the storage box (1), on the outside of the branch pipe (25) there are equidistantly arranged cold air outlets (26), and the cold air outlets (26) are arranged towards the central part inside the storage box (1), on the outside of one end of the ventilation pipe (23) located at the liquid nitrogen tank (22) there is installed a control valve (24).

5. The cell storage device for optimizing cell preservation according to claim 1, wherein: At both ends of the storage box (1) there are symmetrically arranged lifting handles (27).

6. The cell storage device for optimizing cell preservation according to claim 1, characterized in that: At the bottom inside the first vertical frame (4) and the second vertical frame (5), electric telescopic rods (16) are installed, and the end of the piston rod of the electric telescopic rod (16) extends into the lifting groove and is fixedly connected to the lifting plate (6). On the corresponding two side walls of the first vertical frame (4) and the second vertical frame (5), limiting grooves (17) are opened. On the corresponding two side walls of the lifting plate (6), limiting blocks are integrally provided, and the limiting blocks are slidably connected inside the limiting grooves (17).

7. The cell storage device for optimizing cell preservation according to claim 1, wherein: On the corresponding two side walls of the storage box (1), observation windows (29) are provided, and at the four corners of the bottom of the storage box (1), support pads (30) are provided.

8. The cell storage device for optimizing cell preservation according to claim 2, wherein: Counterweight pieces (10) are provided at the bottom of the test tube mounting rack (9).

9. The cell storage device for optimizing cell preservation according to claim 1, characterized in that: At the top of the material loading and unloading port (2), a connection cover (3) is connected in a matching manner, and at the top of the connection cover (3), a control handle (28) is provided.

10. A cell storage method for a cell storage device that optimizes cell preservation according to any one of claims 1-9, characterized in that, It includes the following steps: Step 1: Open the connection cover (3), control the electric telescopic rod (16) to work, drive the test tube mounting rack (9) at the uppermost end of the lifting plate (6) to rise into the material loading and unloading port (2), and quickly place the cell cryopreservation tubes filled with cells one by one on the test tube mounting rack (9); Step 2: Drive the two lifting plates (6) to reset to their original height by the electric telescopic rod (16). At this time, drive the driving motor (15) to work to drive the cross-shaped plate (8) to rotate by 90 degrees, and then cooperate with the electric telescopic rod (16) to work, continue to place the empty test tube mounting rack (9) at the material loading and unloading port (2), place the cell cryopreservation tubes filled with another type of cells again, and then control the lifting plate (6) to reset to its original height again, and the driving motor (15) controls the cross-shaped plate (8) to continue to rotate by 90 degrees, so as to store the cell cryopreservation tubes on all the test tube mounting racks (9) on the cell storage mechanism (13) in sequence; Step 3: After all the placements are completed, the cell storage mechanism (13) resets to its original height, cover the connection cover (3), the liquid nitrogen tank (22) releases cold air into the storage box (1), and at the same time drive the driving motor (15) to work to drive the cross-shaped plate (8) to rotate, so that the positions of different test tube mounting racks (9) change inside the storage box (1), enabling the cells to fully contact the cold air. During the rotation of the cross-shaped plate (8), the indicating rod (20) also rotates accordingly, and the cell type is marked at the label (21) of the indicating rod (20); Step 4: When taking cells, the driving motor (15) can be controlled to rotate so that the cell label (21) to be taken is located at the uppermost part. Open the connection cover (3) and control the electric telescopic rod (16) to work, so that the lifting plate (6) rises to the set height position. At this time, the top of the cell cryopreservation tube is located outside the material loading and unloading port (2), which is convenient for personnel to take. The cell cryopreservation tubes storing other types of cells are located inside the storage box (1) and continue to be frozen. The opening of the material loading and unloading port (2) is small, and the test tube mounting rack (9) blocks the position of the material loading and unloading port (2) after rising, avoiding the leakage of cold air inside the storage box (1).

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