A production process of tea seed essence

Abstract

The present invention belongs to the technical field of biomass extraction. More specifically, it relates to a production process of tea seed essence. The production steps of the present invention include: mixing camellia seed meal and water, and performing ultrasonic impregnation to obtain an impregnation solution; mixing starch and water and gelatinizing to obtain a gelatinized starch solution; adding the gelatinized starch solution to the impregnation solution, mixing evenly, and freezing to obtain a solid mixture; performing cryogenic grinding on the solid mixture to obtain a ground material; completely thawing the ground material to obtain a ground slurry; adding a gelatin solution to the ground slurry and stirring and mixing evenly to obtain a mixed slurry; adjusting the pH of the mixed slurry to obtain an acidified mixed slurry; adding a composite enzyme solution to the acidified mixed slurry, performing an enzymatic hydrolysis reaction, then adding an amylase solution, continuing to keep warm for the enzymatic hydrolysis reaction, inactivating the enzyme, separating, and taking the supernatant; purifying and drying the supernatant to obtain the tea seed essence.

Description

Technical Field

[0001] The present invention belongs to the technical field of biomass extraction. More specifically, it relates to a production process of tea seed essence. Background Art

[0002] Tea saponin, also known as camellia saponin, is a kind of saponin mixture existing in tea seeds and tea leaves seeds. At the same time, tea saponin is also a small molecule substance with very excellent performance and a green natural surfactant. The waste after extracting tea seed oil from camellia oleifera seeds is camellia oleifera seed meal.

[0003] There are various common methods for extracting tea saponin from camellia oleifera seed meal:

[0004] Traditional solvent extraction method, such as ethanol extraction method: using ethanol as the solvent, extracting tea saponin by heating under reflux or soaking, and the yield of tea saponin can reach 17.43% - 26.36%.

[0005] Bio - enzyme method: using bio - enzymes (such as cellulase, protease, lipase, etc.) to decompose impurities in camellia oleifera seed meal, improving the extraction rate and purity of tea saponin. The extraction rate by enzymatic hydrolysis method can reach 86.86%, but the reaction time is relatively long.

[0006] The bio - enzyme method for extracting tea saponin usually requires the synergistic action of multiple enzymes, such as cellulase, protease and lipase, etc. However, there are differences in the optimal action conditions (such as temperature, pH value, enzyme dosage, etc.) of different enzymes, and precise optimization and combination are required to achieve the best extraction effect. In addition, the specificity of enzymes may lead to poor removal effect on some impurities, affecting the purity of tea saponin.

[0007] In addition, although the bio - enzyme method can improve the extraction rate of tea saponin, its extraction efficiency is relatively low and the reaction time is long. For example, the enzymatic hydrolysis time for extracting tea saponin by enzymatic hydrolysis method usually needs more than 60 minutes.

[0008] Based on this, for the production process of extracting tea saponin from camellia oleifera seed meal by the bio - enzyme method, optimization is still needed. Summary of the Invention

[0009] The technical problem to be solved by the present invention is: when using camellia oleifera seed meal as the raw material and adopting the bio - enzyme method to extract tea saponin therefrom, due to the existence of various impurity components in camellia oleifera seed meal, the contact between the bio - enzyme and the target component is affected during the enzymatic hydrolysis reaction, thus affecting the extraction of tea saponin. Based on the above problems, the present invention provides a production process of tea seed essence.

[0010] The object of the present invention is to provide a production process of tea seed essence.

[0011] The above object of the present invention is achieved by the following technical solutions:

[0012] A production process of tea seed essence, and the specific production steps include:

[0013] Freezing and grinding of camellia seed meal:

[0014] Mix camellia seed meal and water at a mass ratio of 1:5 - 10, and perform ultrasonic impregnation for more than 30 minutes to obtain an impregnation solution;

[0015] Mix starch and water at a mass ratio of 1:10 - 12 and gelatinize to obtain a gelatinized starch solution;

[0016] Add the gelatinized starch solution accounting for 10 - 12% of the mass of the impregnation solution to the impregnation solution, mix evenly, and then freeze it into a solid mixture;

[0017] Perform freezing and grinding on the solid mixture to obtain a grinding material;

[0018] Biological enzyme hydrolysis:

[0019] Completely thaw the grinding material to obtain a grinding slurry;

[0020] Add a gelatin solution with a mass fraction of 3 - 5% to the grinding slurry, stir and mix evenly to obtain a mixed slurry; wherein, the dosage of the gelatin solution is 8 - 10% of the mass of the grinding slurry, and the isoelectric point of the gelatin is 7.0;

[0021] Adjust the pH value of the mixed slurry to 4.8 - 5.5 to obtain an acidified mixed slurry;

[0022] Add the compound enzyme solution to the acidified mixed slurry, keep it at a temperature of 50 - 55 °C, carry out an incubation enzymatic hydrolysis reaction for 30 - 35 minutes, then add the amylase solution, continue the incubation enzymatic hydrolysis reaction for 30 - 35 minutes, inactivate the enzyme, and then perform centrifugal separation to take the supernatant;

[0023] In the compound enzyme solution, it includes the following raw materials in parts by weight:

[0024] 30 - 31 parts of lipase, 28 - 30 parts of papain, 3 - 5 parts of trypsin, 32 - 33 parts of cellulase, 300 - 320 parts of water;

[0025] The dosage of the compound enzyme solution is 3 - 5% of the mass of the acidified mixed slurry;

[0026] The amylase solution is an α - amylase solution with a mass fraction of 3 - 5%, and the dosage of the amylase solution is 2 - 4% of the mass of the acidified mixed slurry;

[0027] Purify and dry the supernatant to obtain tea seed essence.

[0028] Advantages of the above technical solution:

[0029] Before the above technical solution adopts enzymatic hydrolysis, the camellia seed meal is subjected to cryogenic grinding. And during the cryogenic grinding process, a starch paste solution is mixed in. The advantage of doing this is that during the gelatinization process of starch, the hydrogen bonds between molecules are broken, causing the starch molecules to gradually dissociate and disperse into water. During this process, the particles absorb a large amount of water and swell, thus forming a uniform dispersion. After mixing with the camellia seed meal, it can be firmly adsorbed and fixed on the surface of the camellia seed meal particles, thereby forming a gelatinized starch film. This gelatinized starch film can significantly improve the water absorption effect of the saponin particles. In this way, during the freezing process, a gelatinized starch ice crystal layer can be formed on the surface of the camellia seed meal particles. During the subsequent cryogenic grinding process, the gelatinized starch ice crystal layer will break under the action of the grinding pressure, thus causing the rapid refinement of the camellia seed meal particles. Thanks to the uniform adsorption of the gelatinized starch ice crystal layer, the refinement process is more uniform, and the morphology and particle size of the particles after fragmentation are also more uniform, thus avoiding the disadvantage that due to the uneven particle size of the camellia seed meal particles after refinement, especially for large particles during the enzymatic hydrolysis process, it takes a long time;

[0030] It should be noted that if there is no gelatinized starch, then due to the residual camellia seed oil on the surface of the camellia seed meal particles, it is difficult to form a good interface with water, and it is very difficult for the ice crystals formed by the freezing of water to assist in the refinement of the camellia seed meal particles during the cryogenic grinding process; More importantly, due to the exposure of the active polar functional groups of the gelatinized starch, it can adsorb and uniformly disperse the fragmented camellia seed meal particles, reducing the sedimentation of the camellia seed meal particles, which helps to a certain extent in the subsequent stable and effective contact with the enzymes directly;

[0031] The inventor further found that the technological process during the enzymatic hydrolysis process also needs to be considered. Mainly because, generally, the enzymatic hydrolysis process needs to adjust the pH to a certain condition. During the process of adjusting the pH, the biomass components such as proteins, fats, and carbohydrates in the camellia seed meal will cause some substances to sediment due to the disruption of the charge balance. During the sedimentation process, some components such as enzymes will also be sedimented together, thus affecting the enzymatic hydrolysis efficiency. In the above technical solution, adding a certain amount of gelatin with an isoelectric point of 7.0 can solve this technical problem. During the process of adjusting the system pH to the range of the above solution, the amino groups in the molecular structure of gelatin are protonated and carry a positive charge. Due to the principle of like charges repelling each other, the molecular chain of gelatin is stretched, and with the synergistic effect of the gelatinized starch molecular chain, the components can be uniformly dispersed. And during the enzymatic hydrolysis process, even if substances such as proteins are continuously released from the camellia seed meal particles, once they are captured by the gelatin molecules, they can also assist in their uniform dispersion, avoiding premature sedimentation and affecting the occurrence of the hydrolysis reaction;

[0032] Gelatin and gelatinized starch will also be gradually hydrolyzed by biological enzymes in the later stage of the hydrolysis process, and their hydrolysis products will not have an adverse effect on the final purity of the product.

[0033] Further, the ultrasonic impregnation includes: maintaining heat and performing ultrasonic impregnation for more than 30 min at a temperature of 35 - 45°C and an ultrasonic frequency of 60 - 80 kHz.

[0034] Further, the gelatinization includes: mixing starch and water at a mass ratio of 1:10 - 12, and then maintaining heat and stirring for gelatinization for 45 - 60 min at a temperature of 90 - 95°C and a stirring speed of 300 - 400 r / min.

[0035] Further, the cryogenic grinding includes:

[0036] In a cryogenic grinder, maintaining cryogenic grinding for 40 - 60 min at a temperature of -20 - -50°C.

[0037] Further, the purification includes:

[0038] Adding acetone accounting for 10 - 12% of the mass of the supernatant to the supernatant, stirring and mixing evenly, then standing for 4 - 6 h, filtering, and collecting the precipitate.

[0039] Further, the cryogenic grinding of the camellia seed meal also includes:

[0040] Subjecting the camellia seed meal to microwave treatment for 30 - 45 min under a microwave power of 300 - 400 W to obtain pretreated camellia seed meal;

[0041] Mixing the pretreated camellia seed meal and water at a mass ratio of 1:5 - 10, and then performing ultrasonic impregnation for more than 30 min to obtain an impregnation solution;

[0042] Mixing starch and water at a mass ratio of 1:10 - 12 and then gelatinizing to obtain a gelatinized starch solution;

[0043] Adding the gelatinized starch solution accounting for 10 - 12% of the mass of the impregnation solution to the impregnation solution, mixing evenly, and then freezing into a solid mixture;

[0044] Performing cryogenic grinding on the solid mixture to obtain a ground material.

[0045] By using microwave treatment, the volatile components in the camellia seed meal can be volatilized during the treatment process. During the volatilization process, the permeability of the plant cells of the camellia seed meal can be increased, which is conducive to the rapid progress of the hydrolysis reaction in the subsequent treatment process.

[0046] Further, the moisture content of the camellia seed meal is 8 - 10%.

[0047] The moisture content is obtained by drying the raw materials in an oven at 90°C until constant weight and measuring the weights of the raw materials before and after drying. Detailed implementation mode

[0048] The following specific examples are used to further illustrate the present invention, but the examples do not limit the present invention in any form. Unless otherwise specified, the reagents, methods and equipment used in the present invention are conventional reagents, methods and equipment in the technical field.

[0049] Unless otherwise specified, the reagents and materials used in the following examples are all commercially available. Example 1

[0050] Freeze-grinding of camellia seed meal:

[0051] Camellia seed meal with a moisture content of 8% is microwave-treated at a microwave power of 300 W for 30 min to obtain pretreated camellia seed meal;

[0052] After mixing the pretreated camellia seed meal and water at a mass ratio of 1:5, keep warm and ultrasonically impregnate for 30 min at a temperature of 35°C and an ultrasonic frequency of 60 kHz to obtain an impregnation solution;

[0053] After mixing starch and water at a mass ratio of 1:10, keep warm and stir and gelatinize for 45 min at a temperature of 90°C and a stirring speed of 300 r / min to obtain a gelatinized starch solution;

[0054] Add the gelatinized starch solution accounting for 10% of the mass of the impregnation solution to the impregnation solution, continuously stir and mix for 3 h at a stirring speed of 300 r / min, and then continuously freeze into a solid mixture at a temperature of -30°C;

[0055] Transfer the solid mixture to a freeze-grinder, and continuously freeze-grind for 40 min at a temperature of -20°C, then discharge to obtain a ground material;

[0056] Biological enzyme hydrolysis:

[0057] Let the ground material stand at room temperature until it is completely thawed to obtain a ground slurry;

[0058] Add a 3% gelatin solution by mass to the ground slurry, and stir and mix with a stirrer at a speed of 300 r / min for 40 min to obtain a mixed slurry; wherein, the dosage of the gelatin solution is 8% of the mass of the ground slurry, and the isoelectric point of the gelatin is 7.0;

[0059] Adjust the pH value of the mixed slurry to 4.8 to obtain an acidified mixed slurry;

[0060] Add the complex enzyme solution to the acidified mixed pulp. Under the conditions of a stirring speed of 80 r / min and a reaction temperature of 50 °C, keep the enzymatic hydrolysis reaction at a constant temperature for 30 min. Then add the amylase solution and continue the enzymatic hydrolysis reaction at a constant temperature for 30 min. Heat up to 75 °C and keep the enzyme inactivated for 10 min. Then perform centrifugal separation and take the supernatant;

[0061] In the complex enzyme solution, it includes the following raw materials in parts by weight:

[0062] 30 parts of lipase, 28 parts of papain, 3 parts of trypsin, 32 parts of cellulase, 300 parts of water;

[0063] The dosage of the complex enzyme solution is 3% of the mass of the acidified mixed pulp;

[0064] The amylase solution is a 3% α-amylase solution by mass fraction, and the dosage of the amylase solution is 2% of the mass of the acidified mixed pulp;

[0065] Add acetone accounting for 10% of the mass of the supernatant to the supernatant, stir and mix at a speed of 200 r / min with a stirrer for 10 min, then let it stand at room temperature for 4 h, filter, collect the precipitate, and dry it to obtain tea seed essence. Example 2

[0066] Freeze-grinding of camellia seed meal:

[0067] Under the condition of a microwave power of 360 W, microwave-treat the camellia seed meal with a moisture content of 9% for 35 min to obtain pretreated camellia seed meal;

[0068] After mixing the pretreated camellia seed meal and water at a mass ratio of 1:8, keep the temperature at 40 °C and the ultrasonic frequency at 70 kHz, and continuously keep the ultrasonic impregnation at a constant temperature for 35 min to obtain an impregnation solution;

[0069] After mixing starch and water at a mass ratio of 1:11, keep the temperature at 92 °C and the stirring speed at 350 r / min, and keep the stirring and gelatinization at a constant temperature for 50 min to obtain a gelatinized starch solution;

[0070] Add the gelatinized starch solution accounting for 11% of the mass of the impregnation solution to the impregnation solution, continuously stir and mix at a stirring speed of 350 r / min for 4 h, and then continuously freeze it into a solid mixture at a temperature of -35 °C;

[0071] Transfer the solid mixture to a freeze-grinder, and continuously freeze-grind it at a temperature of -30 °C for 50 min, then discharge the material to obtain a ground material;

[0072] Biological enzyme hydrolysis:

[0073] Let the ground material stand at room temperature until it is completely thawed to obtain a ground pulp;

[0074] Add a gelatin solution with a mass fraction of 4% to the grinding slurry, and stir and mix it with a stirrer at a speed of 350 r / min for 50 min to obtain a mixed slurry; wherein, the dosage of the gelatin solution is 9% of the mass of the grinding slurry, and the isoelectric point of the gelatin is 7.0;

[0075] Adjust the pH value of the mixed slurry to 5.2 to obtain an acidified mixed slurry;

[0076] Add the compound enzyme solution to the acidified mixed slurry, and under the conditions of a stirring speed of 90 r / min and a reaction temperature of 52 °C, keep the temperature for enzymatic hydrolysis reaction for 32 min, then add the amylase solution, continue to keep the temperature for enzymatic hydrolysis reaction for 32 min, heat up to 75 °C, keep the temperature for enzyme inactivation for 10 min, and then perform centrifugal separation to take the supernatant;

[0077] In the compound enzyme solution, it includes the following raw materials in parts by weight:

[0078] 30 parts of lipase, 29 parts of papain, 4 parts of trypsin, 32 parts of cellulase, 310 parts of water;

[0079] The dosage of the compound enzyme solution is 4% of the mass of the acidified mixed slurry;

[0080] The amylase solution is an α - amylase solution with a mass fraction of 4%, and the dosage of the amylase solution is 3% of the mass of the acidified mixed slurry;

[0081] Add acetone accounting for 11% of the mass of the supernatant to the supernatant, stir and mix it with a stirrer at a speed of 200 r / min for 10 min, then let it stand at room temperature for 5 h, filter, collect the precipitate, and dry it to obtain tea seed essence. Example 3

[0082] Freeze - grinding of camellia seed meal:

[0083] Subject the camellia seed meal with a moisture content of 10% to microwave treatment at a microwave power of 400 W for 45 min to obtain pretreated camellia seed meal;

[0084] Mix the pretreated camellia seed meal and water in a mass ratio of 1:10, and under the conditions of a temperature of 45 °C and an ultrasonic frequency of 80 kHz, continuously keep the temperature for ultrasonic impregnation for 50 min to obtain an impregnation solution;

[0085] Mix starch and water in a mass ratio of 1:12, and under the conditions of a temperature of 95 °C and a stirring speed of 400 r / min, keep the temperature for stirring and gelatinization for 60 min to obtain a gelatinized starch solution;

[0086] Add a gelatinized starch solution accounting for 12% of the mass of the impregnating solution thereto, and under the condition that the stirring speed is 400 r / min, continuously stir and mix for 5 h, and then under the condition that the temperature is -45°C, continuously freeze to form a solid mixture;

[0087] Transfer the solid mixture to a cryogenic grinder, and under the condition that the temperature is -50°C, continuously cryogenically grind for 60 min, and discharge the material to obtain a ground material;

[0088] Biological enzyme hydrolysis:

[0089] Let the ground material stand at room temperature until it is completely thawed to obtain a ground slurry;

[0090] Add a 5% gelatin solution by mass to the ground slurry, and use a stirrer to stir and mix at a speed of 400 r / min for 60 min to obtain a mixed slurry; wherein, the dosage of the gelatin solution is 10% of the mass of the ground slurry, and the isoelectric point of the gelatin is 7.0;

[0091] Adjust the pH value of the mixed slurry to 5.5 to obtain an acidified mixed slurry;

[0092] Add the complex enzyme solution to the acidified mixed slurry, under the conditions that the stirring speed is 120 r / min and the reaction temperature is 55°C, keep the temperature for enzymatic hydrolysis reaction for 35 min, then add the amylase solution, continue to keep the temperature for enzymatic hydrolysis reaction for 35 min, then heat up to 75°C, keep the temperature for enzyme inactivation for 10 min, and then perform centrifugal separation to take the supernatant;

[0093] In the complex enzyme solution, it includes the following raw materials in parts by weight:

[0094] 31 parts of lipase, 30 parts of papain, 5 parts of trypsin, 33 parts of cellulase, 320 parts of water;

[0095] The dosage of the complex enzyme solution is 5% of the mass of the acidified mixed slurry;

[0096] The amylase solution is a 5% α-amylase solution by mass, and the dosage of the amylase solution is 4% of the mass of the acidified mixed slurry;

[0097] Add acetone accounting for 12% of the mass of the supernatant thereto, use a stirrer to stir and mix at a speed of 200 r / min for 10 min, then let it stand at room temperature for 6 h, filter, collect the precipitate, and dry it to obtain tea seed essence. Example 4

[0098] Compared with Example 1, this example is different in that: microwave treatment is not carried out, and the other conditions remain unchanged.

[0099] Comparative Example 1

[0100] The difference between this comparative example and Example 1 is that gelatinized starch solution was not added, and the other conditions remained unchanged.

[0101] Comparative Example 2

[0102] The difference between this comparative example and Example 1 is that cryogenic grinding was not used. Specifically:

[0103] Tea seed meal with a moisture content of 8% was microwave-treated at a microwave power of 300 W for 30 min to obtain pretreated tea seed meal.

[0104] The pretreated tea seed meal and water were mixed at a mass ratio of 1:5, and then kept warm and ultrasonically impregnated at a temperature of 35 °C and an ultrasonic frequency of 60 kHz for 30 min to obtain an impregnation solution.

[0105] Starch and water were mixed at a mass ratio of 1:10, and then kept warm and stirred for gelatinization at a temperature of 90 °C and a stirring speed of 300 r / min for 45 min to obtain gelatinized starch solution.

[0106] 10% of the gelatinized starch solution based on the mass of the impregnation solution was added to the impregnation solution, and then continuously stirred and mixed at a stirring speed of 300 r / min for 3 h. Then, it was continuously ground in a grinder at room temperature for 40 min, and the product was discharged to obtain the ground material.

[0107] The other conditions remained unchanged.

[0108] Comparative Example 3

[0109] The difference between this comparative example and Example 1 is that gelatin solution was not added, and the other conditions remained unchanged.

[0110] The products obtained in the above examples and comparative examples were subjected to performance tests. The specific test methods and test results are as follows:

[0111] The purity of tea saponin in the products obtained in each example and comparative example was tested by high performance liquid chromatography. The specific test conditions were: C18 column, mobile phase was methanol-water (volume ratio 9:1), detection wavelength was 280 nm. The specific test results are shown in Table 1.

[0112] ;

[0113] It can be seen from the test results in Table 1 that the biological enzyme hydrolysis method adopted in the present invention can obtain products with relatively high purity. And during the hydrolysis process, it was detected that during the extraction process of Examples 1-4, especially during the hydrolysis stage, no obvious flocculent precipitates appeared, while certain degrees of sedimentation occurred in the comparative examples, which affected the progress of the hydrolysis reaction.

[0114] The above embodiments are preferred embodiments of the present invention, but the embodiments of the present invention are not limited to the above embodiments. Any other changes, modifications, substitutions, combinations, or simplifications made without departing from the spirit and principle of the present invention shall be equivalent replacement methods and are all included in the protection scope of the present invention.

Claims

1. A production process of tea seed essence, characterized in that, The specific production steps include: Freeze-grinding of camellia seed meal: Mix camellia seed meal and water at a mass ratio of 1:5 - 10, and perform ultrasonic impregnation for more than 30 min to obtain an impregnation solution; Mix starch and water at a mass ratio of 1:10 - 12 and gelatinize to obtain a gelatinized starch solution; Add the gelatinized starch solution accounting for 10 - 12% of the mass of the impregnation solution to the impregnation solution. After mixing evenly, freeze it into a solid mixture; Perform freeze-grinding on the solid mixture to obtain a ground material; Biological enzyme hydrolysis: Completely thaw the ground material to obtain a ground slurry; Add a gelatin solution with a mass fraction of 3 - 5% to the ground slurry and stir to mix evenly to obtain a mixed slurry; wherein, the dosage of the gelatin solution is 8 - 10% of the mass of the ground slurry, and the isoelectric point of the gelatin is 7.0; Adjust the pH value of the mixed slurry to 4.8 - 5.5 to obtain an acidified mixed slurry; Add the complex enzyme solution to the acidified mixed slurry. Under the condition of a temperature of 50 - 55 °C, keep the temperature for enzymatic hydrolysis reaction for 30 - 35 min, then add the amylase solution, continue to keep the temperature for enzymatic hydrolysis reaction for 30 - 35 min, inactivate the enzyme, and then perform centrifugal separation to take the supernatant; In the complex enzyme solution, it includes the following raw materials in parts by weight: 30 - 31 parts of lipase, 28 - 30 parts of papain, 3 - 5 parts of trypsin, 32 - 33 parts of cellulase, 300 - 320 parts of water; The dosage of the complex enzyme solution is 3 - 5% of the mass of the acidified mixed slurry; The amylase solution is an α - amylase solution with a mass fraction of 3 - 5%, and the dosage of the amylase solution is 2 - 4% of the mass of the acidified mixed slurry; Purify and dry the supernatant to obtain tea seed essence.

2. The production process of a tea seed essence according to claim 1, characterized in that, The ultrasonic impregnation includes: Under the conditions of a temperature of 35 - 45 °C and an ultrasonic frequency of 60 - 80 kHz, continuously keep the temperature for ultrasonic impregnation for more than 30 min.

3. The production process of a tea seed essence according to claim 1, characterized in that, The gelatinization includes: Mix starch and water at a mass ratio of 1:10 - 12, and under the conditions of a temperature of 90 - 95 °C and a stirring speed of 300 - 400 r / min, keep the temperature for stirring and gelatinization for 45 - 60 min.

4. The production process of a tea seed essence according to claim 1, characterized in that, The freeze-grinding includes: In a freeze-grinder, under the condition of a temperature of - 20 - - 50 °C, continuously perform freeze-grinding for 40 - 60 min.

5. The production process of a tea seed essence according to claim 1, characterized in that, The purification includes: Add acetone accounting for 10 - 12% of the mass of the supernatant to the supernatant, stir to mix evenly, stand for 4 - 6 h, filter, and collect the precipitate.

6. The production process of a tea seed essence according to claim 1, characterized in that, The freeze-grinding of the camellia seed meal also includes: Perform microwave treatment on the camellia seed meal under the condition of a microwave power of 300 - 400 W for 30 - 45 min to obtain pretreated camellia seed meal; Mix the pretreated camellia seed meal and water at a mass ratio of 1:5 - 10, and perform ultrasonic impregnation for more than 30 min to obtain an impregnation solution; Mix starch and water at a mass ratio of 1:10 - 12 and gelatinize to obtain a gelatinized starch solution; Add the gelatinized starch solution accounting for 10 - 12% of the mass of the impregnation solution to the impregnation solution. After mixing evenly, freeze it into a solid mixture; Perform freeze-grinding on the solid mixture to obtain a ground material.

7. The production process of a tea seed essence according to claim 6, characterized in that, The moisture content in the camellia seed meal is 8 - 10%.