Biological cell lysis system based on ultrasonic driving

Abstract

The invention discloses a biological cell lysis system based on ultrasonic driving. The device belongs to the technical field of mutual intersection of ultrasonic and biological cell treatment, and comprises an ultrasonic amplitude rod, a Langevin vibrator, a bracket, a container and a battery, the Langevin vibrator is connected to the support, the ultrasonic amplitude rod is connected with the Langevin vibrator, the battery is connected to the platform, and the Langevin vibrator is electrically connected to the battery; a groove is formed in the ultrasonic amplitude rod, and a container is placed in the groove; when the battery supplies power to the Langevin vibrator, an ultrasonic signal generated by the Langevin vibrator is transmitted to the ultrasonic amplitude rod, the ultrasonic amplitude rod transmits sound waves into the container, when the sound waves are transmitted to biological cells, secondary sound streams are generated, the secondary sound streams generate vortexes in the movement process, and when the vortexes collide with vortexes generated by the cells, the vortexes of the cells collide with the vortexes generated by the cells. The cell membrane is broken. Biological cell lysis is realized in an ultrasonic driving manner, and the used device has the advantages of easiness in miniaturization, easiness in cleaning, easiness in treatment and the like.

Description

Technical Field

[0001] The invention belongs to the technical field of ultrasound and biological cell processing, and relates to an ultrasound-driven biological cell lysis system. Background Art

[0002] Biological cell lysis refers to the destruction of cell membranes by physical, chemical or enzymatic methods to release intracellular components. It is the basis for separating and purifying non-secretory biochemical substances (products) synthesized in cells. Cell lysis is an important experimental method to change cell permeability and activity, and is widely used in many biochemical experiments and tests. For example, protein extraction and analysis, metabolomics research, cell apoptosis and necrosis research, etc. At present, the more traditional cell lysis methods are grinding and ball stirring. The advantages of the two methods are that they are usually more efficient, can completely lyse cells, and are suitable for large-scale cell processing, but they also have a major defect, which is that they may cause damage to DNA in cells, affecting subsequent analysis.

[0003] At present, there are some methods to deal with the disadvantage of traditional cell lysis methods causing damage to intracellular DNA. (Chinese patent, patent number: CN 119040248) provides a method for lysing cells by continuous flow atomization at room temperature. This method also uses chemical lysis method, only a small amount of lysis solution is needed for cell lysis, which improves the speed, yield and abundance of cell lysis. However, this method cannot avoid the contamination of the cell solution by the chemical solution, and the implementation process is relatively complicated and cannot be widely used.

[0004] Therefore, it is necessary to improve the existing technology to solve the shortcomings of the existing technology. Summary of the invention

[0005] In view of the above problems, the present invention aims to propose a biological cell lysis system driven by ultrasound.

[0006] The technical solution of the present invention is: the ultrasonic-driven biological cell lysis system described in the present invention comprises a platform of the overall device, wherein the ultrasonic amplitude rod is connected to the bottom of the Langevin vibrator;

[0007] The container is placed in the groove of the ultrasonic amplitude rod, and the battery is placed on the platform.

[0008] Furthermore, batteries are installed on the platform, and the types of the batteries include but are not limited to cylindrical batteries, square batteries and storage batteries.

[0009] Furthermore, the Langevin vibrator is bonded to the upper part of the bracket by epoxy resin;

[0010] The ultrasonic amplitude rod is bonded to the bottom of the Langevin vibrator by epoxy resin.

[0011] The shape of the cross section of the ultrasonic amplitude rod includes but is not limited to square, circle, pentagon and the like.

[0012] Furthermore, the excitation frequency, initial phase, vibration amplitude, and waveform of the Langevin vibrator are adjustable, wherein the excitation frequency is greater than or equal to 20kHz, and the vibration amplitude is at the micrometer or sub-millimeter level;

[0013] The materials used to make it include but are not limited to piezoelectric ceramics, single crystals, composite materials and magnetostrictive materials.

[0014] Furthermore, the bracket is connected with a magnetic contact A, and the magnetic contact A is connected to the battery through a cable;

[0015] The Langevin vibrator is connected to a wire made of hard copper wire, one end of which is connected to a magnetic contact B. When the Langevin vibrator is pressed down to a certain position, the magnetic contact A and the magnetic contact B will automatically attract each other to provide power to the Langevin vibrator; when the Langevin vibrator is lifted up to a certain position, the magnetic contact A and the magnetic contact B will also separate and cut off the power due to the existence of pulling force.

[0016] Furthermore, a plurality of grooves are formed on the side of the ultrasonic amplitude rod, and the grooves are evenly arranged on the side of the ultrasonic amplitude rod, and the grooves are of equal size and have the same spacing therebetween; the placement holes on the grooves are circular, and threads are provided in the grooves.

[0017] Furthermore, the container is made of corrosion-resistant materials, including but not limited to glass, polymer materials or hydrophilic and hydrophobic materials, and is in the shape of a circular tube; its diameter is the same as the inner diameter of the groove;

[0018] The container is sealed by a piston, and a thread is provided at the top of the container, which is the same as the thread in the groove on the ultrasonic amplitude rod. The thread can ensure that the container is stably connected to the groove of the ultrasonic amplitude rod.

[0019] Further, the volume of the container is 25ml-30ml;

[0020] The types of solutions in the container include, but are not limited to, human or animal serum and plant cell solutions.

[0021] The beneficial effects of the present invention are: 1. It has a simple structure, low cost, good stability, and low difficulty in use, which is conducive to wide application; 2. It will not cause damage to DNA in cells, has better safety, and is more energy-saving and environmentally friendly; 3. The application of ultrasonic drive to the miniaturization of biological cell lysis can provide possibilities for it, help promote its practical application, and provide a theoretical basis for its commercialization. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0023] Figure 2 It is a schematic diagram of the structure of the ultrasonic amplitude rod in the present invention;

[0024] In the figure: 1 is a bracket, 2 is a Langevin vibrator, 3 is an ultrasonic amplitude rod, 301 is a groove, 4 is a container, 5 is a platform, 6 is a battery, 7 is a magnetic contact, and 8 is a wire. DETAILED DESCRIPTION

[0025] The specific technical scheme of the present invention is further described in detail below with reference to specific examples.

[0026] As shown in the figure, the ultrasound-driven biological cell lysis system of the present invention includes a bracket 1, a Langevin vibrator 2, an ultrasonic amplitude rod 3, a container 4, a platform 5, a battery 6, a magnetic contact 7 and a wire 8;

[0027] The Langevin vibrator 2 is connected to the bracket 1, the ultrasonic amplitude rod 3 is connected to the Langevin vibrator 2 through the bracket 1, the battery 6 is connected to the platform 5 at the bottom of the bracket 1, and the Langevin vibrator 2 can be electrically connected to the battery 6;

[0028] A groove 301 is formed on the ultrasonic amplitude rod 3, and a plurality of placement holes are evenly formed in the groove 301 for placing the sample container 4;

[0029] When the battery 6 supplies power to the Langevin vibrator 2, the ultrasonic signal generated by the Langevin vibrator 2 is transmitted to the ultrasonic amplitude rod 3, and the ultrasonic amplitude rod 3 propagates the sound wave into the container 4. When the sound wave is transmitted to the biological cell, a secondary sound current is generated. The secondary sound current generates a vortex during the movement. When the vortex collides with the vortex generated by the cell itself, the cell membrane will be ruptured.

[0030] A bracket 1 is arranged on the upper wall of the platform 5 of the overall device, and the bracket 1 is L-shaped;

[0031] A Langevin vibrator 2 is arranged at the front end of the bracket 1, and an ultrasonic amplitude rod 3 is connected to the bottom of the Langevin vibrator 2;

[0032] The container 4 is placed in the groove 301 of the ultrasonic amplitude rod 3 , and the battery 6 is placed on the platform 5 .

[0033] A battery 6 is disposed on the platform 5 , and types of the battery 6 include but are not limited to cylindrical batteries, square batteries and storage batteries.

[0034] The Langevin vibrator 2 is bonded to the upper part of the bracket 1 by epoxy resin;

[0035] The ultrasonic amplitude rod 3 is bonded to the bottom of the Langevin vibrator 2 by epoxy resin, and the shape of the cross section of the ultrasonic amplitude rod 3 includes but is not limited to square, circle, pentagon and the like.

[0036] The excitation frequency, initial phase, vibration amplitude, and waveform of the Langevin vibrator 2 are adjustable, wherein the excitation frequency is greater than or equal to 20kHz, and the vibration amplitude is at the micrometer or sub-millimeter level;

[0037] The materials used to make it include but are not limited to piezoelectric ceramics, single crystals, composite materials and magnetostrictive materials.

[0038] The bracket 1 is connected to a magnetic contact A, which is connected to a battery 6 via a cable;

[0039] The Langevin vibrator 2 is connected to a wire 8 made of hard copper wire, and one end of the wire 8 is connected to a magnetic contact B. When the Langevin vibrator 2 is pressed downward to a certain position, the magnetic contact A and the magnetic contact B will automatically attract each other to supply power to the Langevin vibrator 2; when the Langevin vibrator 2 is lifted upward to a certain position, the magnetic contact A and the magnetic contact B will also separate and cut off the power due to the existence of pulling force.

[0040] Several grooves 301 are opened on the side of the ultrasonic amplitude rod 3. These grooves 301 are evenly arranged on the side of the ultrasonic amplitude rod 3, and the sizes of these grooves 301 are equal and the intervals between them are also the same; the placement holes on the grooves 301 are circular, and threads are provided in the grooves 301.

[0041] The container 4 is made of corrosion-resistant materials, including but not limited to glass, polymer materials or hydrophilic and hydrophobic materials, and is generally in the shape of a round tube; made of glass or plastic; and has a diameter that is the same as the inner diameter of the groove 301.

[0042] The container 4 is sealed by a piston, and a thread is provided at the top of the container 4, which is the same as the thread in the groove 301 on the ultrasonic amplitude rod 3. The thread can ensure that the container 4 is stably connected to the groove 301 of the ultrasonic amplitude rod 3;

[0043] The volume of the container 4 is 25ml-30ml; the types of solutions in the container 4 include but are not limited to human or animal serum and plant cell solutions.

[0044] In the present invention, the bottom diameter and height of the container 4 are 8 cm and 25 cm respectively. The Langevin vibrator 2 is bonded to the top of the bracket 1 by epoxy resin, and its height is 35 cm and its diameter is φ30 cm. In the experiment, it can be known by the frequency sweeping method that the resonance frequency of the Langevin vibrator 2 is between 100 kHz and 600 kHz, and is used as the working frequency in the experiment; the length of the ultrasonic amplitude rod 3 is 80 cm, and the cross section is a square with a side length of 30 cm;

[0045] The motor 9 uses a DC servo motor with a power of 150-350kw; three rows of grooves 301 are opened on the side of the ultrasonic amplitude rod 3, and the spacing between each row of grooves 301 is 20cm. The inner diameter is 8cm, which is the same as the cross-sectional diameter of the container 4, and the depth is 5cm. A thread is provided therein to facilitate the stability of the container 4; in this work, the solution in the container 4 is animal serum, and the concentrations are 1M, 2M, 3M, 4M and 5M respectively; at the beginning of the experiment, 10ml of animal serum is added to each container 4, and then these containers 4 are placed on the side of the ultrasonic amplitude rod 3; the Langevin vibrator 2 is pressed, the magnetic contact A is connected to the magnetic contact B, the Langevin vibrator 2 is powered, and the ultrasonic amplitude rod 3 is driven to propagate ultrasonic waves into the container 4. After 5 minutes, the cell rupture in the container is observed under a microscope to determine the cell lysis efficiency of the method.

[0046] The invention can be widely used in the fields of biological research and medicine. In the field of biological research, cell lysis can be used to extract proteins in cells for subsequent functional analysis, structural research and interaction research. In the field of medicine, it can assist in biomarker detection, extract biomarkers by lysing cells, and use them for early diagnosis and monitoring of diseases. Compared with the traditional cell lysis method, the container 4 is placed in the ultrasonic amplitude rod 3, which makes the transmission efficiency higher, does not cause damage to the DNA in the cells, and is more energy-saving and environmentally friendly.

Claims

1. A biological cell lysis system driven by ultrasound, characterized in that: The device comprises a platform (5) of the whole device, a bracket (1) is installed on the upper wall of the platform (5), and a Langevin vibrator (2) and a magnetic contact (7) are installed on the upper end of the bracket (1). The magnetic contact (7) comprises a magnetic contact A and a magnetic contact B; A conductor (8) made of hard copper wire is connected to the Langevin vibrator (2), and the other end of the conductor (8) is connected to a magnetic attraction contact B.

2. The ultrasound-driven biological cell lysis system according to claim 1, characterized in that: An ultrasonic amplitude rod (3) is arranged at the bottom of the Langevin vibrator (2), a plurality of grooves (301) are evenly arranged on four sides of the ultrasonic amplitude rod (3), and containers (4) are arranged in each of the grooves (301).

3. The ultrasound-driven biological cell lysis system according to claim 1, characterized in that: The Langevin vibrator (2) is bonded to the upper side of the bracket (1) by epoxy resin; The ultrasonic amplitude rod (3) is bonded to the bottom of the Langevin vibrator (2) by epoxy resin; The shape of the cross section of the ultrasonic amplitude rod (3) includes but is not limited to a square, a circle or a pentagon.

4. The ultrasound-driven biological cell lysis system according to claim 1, characterized in that: A battery (6) is also installed on the platform (5), and the magnetic contact point A is connected to the battery (6) via a cable.

5. The ultrasound-driven biological cell lysis system according to claim 4, characterized in that: The types of the battery (6) include but are not limited to cylindrical batteries, square batteries and storage batteries.

6. The ultrasound-driven biological cell lysis system according to claim 1, characterized in that: The waveform parameters of the excitation frequency, initial phase and vibration amplitude of the Langevin vibrator (2) are adjustable; The excitation frequency is greater than or equal to 20kHz, and the vibration amplitude is at the micrometer or sub-millimeter level. The manufacturing materials include but are not limited to piezoelectric ceramics, single crystals, composite materials and magnetostrictive materials.

7. The ultrasound-driven biological cell lysis system according to claim 2, characterized in that: The sizes of the grooves (301) and the intervals between them are equal and the same; The placement hole formed on the groove (301) is circular, and a thread is formed in the groove (301).

8. The ultrasound-driven biological cell lysis system according to claim 7, characterized in that: The container (4) is made of corrosion-resistant materials, including but not limited to glass, polymer materials or hydrophilic and hydrophobic materials, and is in the shape of a circular tube; Its diameter is the same as the inner diameter of the groove.

9. The ultrasound-driven biological cell lysis system according to claim 8, characterized in that: The container (4) is sealed by a piston, and a thread is provided at the top of the container (4), which is the same as the thread in the groove (301).

10. The ultrasound-driven biological cell lysis system according to claim 8, characterized in that: The volume of the container (4) is 25ml-30ml; The types of solutions in the container (4) include, but are not limited to, human or animal serum and plant cell solutions.

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