An efficient detection device and method for the bacterial content in the pipe string of a shale gas extraction system

Abstract

The present invention discloses an efficient detection device and method for the bacterial content in a pipe string based on a shale gas extraction system. The present invention relates to the technical field of detecting bacterial content. It includes a machine platform supported on the ground, and an auxiliary detection component arranged on the machine platform for collecting sample water, automatically separating bacteria in the sample water alone, and automatically shaking reagents. A liquid adding and light shielding component is also arranged on the machine platform on the right side of the auxiliary detection component; the right end of the rotating shaft is provided with a biofilm installation assembly directly below the straight cylinder. The beneficial effects of the present invention are: reducing the working intensity of workers, greatly improving the detection efficiency of the bacterial content in the pipe string, and being able to timely change the displacement of the variable pump.

Description

Technical Field

[0001] The present invention relates to the technical field of detecting the content of bacteria, in particular to an efficient detection device and method for the content of bacteria in the pipe string of a shale gas extraction system. Background Art

[0002] The structure of the shale gas extraction system used in a certain area is as Figure 1 shown. It is used to extract the shale gas underground. The shale gas extraction system includes a wellbore 1 fixed in the well, a Christmas tree 2 fixed on the top surface of the wellbore 1. A pipe string 3 is arranged in the wellbore 1, and the top end of the pipe string 3 is fixed on the Christmas tree 2. A gas-liquid separator 5 is connected to the end valve 4 of the Christmas tree 2. A gas transmission pipeline 6 and a drainage pipeline 7 are connected to the gas-liquid separator 5. A first stop valve 8 is connected to the drainage pipeline 7. A desander 9 is connected to the end port of the first stop valve 8. A second stop valve 10 is connected to the end port of the desander 9. A water outlet pipe 11 is connected to the other port of the second stop valve 10. In the initial state, both the first stop valve 8 and the second stop valve 10 are in the closed state;

[0003] The shale gas extraction system further includes a variable pump 12 and a bactericidal corrosion inhibitor storage tank 13 arranged on the ground. The liquid suction port of the variable pump 12 is connected to the bactericidal corrosion inhibitor storage tank 13. A bent pipe 14 is connected to the liquid discharge port of the variable pump 12. The other end of the bent pipe 14 penetrates through the side wall of the wellbore 1 and extends below the pipe string 3. An atomizing ball head 15 is connected to the extending end of the bent pipe 14.

[0004] The method for the shale gas extraction system to extract the shale gas underground is as follows:

[0005] S1. Open the variable pump 12. The variable pump 12 pumps out the bactericidal corrosion inhibitor in the bactericidal corrosion inhibitor storage tank 13. Under the pump pressure, the bactericidal corrosion inhibitor sequentially passes through the variable pump 12, the bent pipe 14, and the atomizing ball head 15, and finally sprays out from the holes on the atomizing ball head 15. The sprayed bactericidal corrosion inhibitor enters the pipe string 3;

[0006] S2. Open the end valve 4 of the Christmas tree and the gas-liquid separator 5. At this time, the shale gas in the production layer enters the wellbore 1. Then, under the gas pressure, the shale gas sequentially flows through the bottom port of the pipe string 3, the inner cavity of the pipe string 3, the Christmas tree 2, the end valve 4, and finally enters the gas-liquid separator 5. The flow direction of the shale gas is as Figure 1 shown by the arrow in the figure. Among them, when the shale gas enters the pipe string 3, the shale gas is mixed with the bactericidal corrosion inhibitor sprayed into the pipe string 3. The bactericidal corrosion inhibitor kills the bacteria entrained in the shale gas, thus effectively avoiding the corrosion of the pipe string 3 by bacteria (the bacteria include iron bacteria, saprophytic bacteria, and sulfate-reducing bacteria), and preventing the occurrence of perforation phenomena on the pipe string 3, thereby protecting the pipe string 3;

[0007] S3. The gas-water separator 5 separates the water entrained in the shale gas. The water-free shale gas is then transported through the gas pipeline 6 to the subsequent section; while the separated water enters the drainage pipeline 7, and the drainage pipeline 7 discharges the water into the sewage treatment equipment;

[0008] S4. After the shale gas is mined for a period of time, workers need to regularly detect the content of bacteria in the pipe string 3. Workers change the displacement of the variable pump 12 according to the detected bacteria content, so as to accurately inject bactericidal and corrosion inhibitors into the pipe string 3, thereby protecting the pipe string 3 and saving the usage amount of bactericidal and corrosion inhibitors. The method for workers to regularly detect the bacteria content in the pipe string 3 is as follows:

[0009] S41. Sampling of the sample water: Workers place the sampling container 16 directly below the water outlet pipe 11 of the shale gas extraction system, as Figure 2 shown; then open the first stop valve 8, and the water in the drainage pipeline 7 is diverted to the desander 9 through the first stop valve 8, and then close the first stop valve 8; the desander 9 removes the sand and oil in the water. After standing for a period of time, the desander 9 purifies the water; then open the second stop valve 10, and the purified water in the desander 9 flows into the sampling container 16 through the second stop valve 10, the water outlet pipe 11 in sequence, thus completing the sampling of the sample water;

[0010] S42. Workers take out a filtering container as Figure 3 shown. The filtering container includes a container body 17 and an end cover 18 connected to the bottom of the container body 17. A biological membrane 19 is fixedly arranged between the end cover 18 and the container body 17, and a through groove 20 is opened in the middle of the end cover 18;

[0011] S43. Separating the bacteria in the sample water: Workers pour the sample water in the sampling container 16 into the container body 17 of the filtering container. The water molecules of the sample water sequentially pass through the biological membrane 19 and the through groove 20 and finally fall to the ground. The flow direction of the water molecules is as Figure 4 shown by the arrow in the figure, while the bacteria in the sample water are intercepted on the top surface of the biological membrane 19 because they cannot pass through the biological membrane 19, thereby separating the bacteria in the sample water. At this time, a layer of bacteria covers the top surface of the biological membrane 19;

[0012] S44. Workers embed the plug 21 into the through groove 20 of the end cover 18 to block the through groove 20, as Figure 5 shown; then add lysis reagent and stabilizer into the container body 17 of the filtering container in sequence, and the adding direction is as Figure 6As shown by the solid arrow in the middle; after adding, the worker shakes the container body 17 to mix the lysis reagent, the stabilizer and the bacteria. Among them, the lysis reagent is used to destroy the bacteria so that ATP (adenosine triphosphate) inside the bacteria is released, and the stabilizer is used to stabilize ATP to prevent ATP degradation;

[0013] S45. The worker adds a certain amount of luciferase reagent into the container body 17 of the filtering container. The luciferase reagent reacts with ATP to generate a fluorescent substance, and the fluorescent substance emits light;

[0014] S46. The worker covers the top port of the container body 17 with the light-shielding cover plate 22, as Figure 7 shown, to prevent external natural light from entering the container body 17 and to avoid the detection accuracy of the photometer; then the photosensitive probe 24 of the photometer 23 is inserted into the light-shielding cover plate 22, as Figure 8 shown. The photosensitive probe 24 detects the intensity of the light in the container body 17. Since the light intensity is proportional to the content of bacteria, the content of bacteria in the pipe column 3 is finally detected;

[0015] S47. After the detection is completed, the worker pours the fluorescent substance and the residual reagent in the container body 17 of the filtering container into the designated waste liquid tank to prepare for the next detection;

[0016] S5. When the detected bacteria content is higher than the specified content, it means that the number of bacteria entering the pipe column 3 is large. At this time, the worker increases the displacement of the variable pump 12, and then increases the amount of the bactericidal corrosion inhibitor sprayed into the pipe column 3 to prevent the bacteria from corroding the pipe column 3, thereby protecting the pipe column 3; when the detected bacteria content is lower than the specified content, it means that the number of bacteria entering the pipe column 3 is small. At this time, the worker reduces the displacement of the variable pump 12, and then reduces the amount of the bactericidal corrosion inhibitor sprayed into the pipe column 3, thereby saving the usage amount of the bactericidal corrosion inhibitor.

[0017] However, in step S4, although the worker can detect the content of bacteria, there are still the following technical defects in the actual operation:

[0018] I. In step S43, workers need to manually pour the sample water in the sampling container 16 into the container body 17 of the filtering container to separately isolate the bacteria in the sample water; in step S44, workers need to manually use the plugging head 21 to plug the through groove 20 of the end cap 18; in step S44, workers need to shake the container body 17 to mix the lysis reagent, stabilizer and bacteria; in steps S44 - S45, workers need to sequentially add the lysis reagent, stabilizer and luciferase reagent into the container body 17 of the filtering container to react and generate fluorescent substances; in step S46, workers also need to use the light - shielding cover plate 22 to cover the top port of the container body 17 of the filtering container, and workers also need to insert the photosensitive probe 24 of the photometer 23 into the light - shielding cover plate 22 to detect the light intensity through the photometer 23, and then finally detect the content of bacteria in the pipe column 3.

[0019] During the whole detection process, it is all manually operated by workers, and the detection is not continuous. This not only increases the working intensity of workers, but also causes a long time to complete the detection of the bacteria content once, thus reducing the detection efficiency of the bacteria content in the pipe column 3, and then resulting in the inability to timely change the displacement of the variable pump 12, still failing to achieve the purpose of protecting the pipe column 3 and saving the usage amount of the bactericidal corrosion inhibitor.

[0020] II. In step S47, after the detection is completed, workers need to turn the filtering container above the waste liquid tank, and then pour all the fluorescent substances and residual reagents in the container body 17 into the waste liquid tank. The whole pouring process is also manually operated by workers, thus increasing the working intensity of workers.

[0021] Therefore, there is an urgent need for an efficient detection device and method that can reduce the working intensity of workers, greatly improve the detection efficiency of the bacteria content in the pipe column 3, and can timely change the displacement of the variable pump. Summary of the Invention

[0022] The purpose of the present invention is to overcome the shortcomings of the prior art and provide an efficient detection device and method for the bacteria content in the pipe column based on the shale gas extraction system, which can reduce the working intensity of workers, greatly improve the detection efficiency of the bacteria content in the pipe column, and can timely change the displacement of the variable pump.

[0023] The purpose of the present invention is achieved by the following technical solutions: An efficient detection device for the bacteria content in the pipe column based on the shale gas extraction system, which includes a machine platform supported on the ground, and an auxiliary detection component arranged on the machine platform for collecting sample water, automatically separating the bacteria in the sample water alone, and automatically shaking the reagents. There is also a liquid - adding and light - shielding component arranged on the machine platform on the right side of the auxiliary detection component;

[0024] The auxiliary detection assembly includes a drive assembly fixed on the machine table. A mounting plate (L-plate) is fixed on the top of the drive assembly. A feed oil cylinder is fixed on the L-plate. The piston rod of the feed oil cylinder penetrates through the L-plate to the left, and a movable plate is fixed on the extending end. A straight cylinder is hinged to the left side of the movable plate through a hinge seat. A reciprocating oil cylinder is fixed on the right side of the movable plate. The piston rod of the reciprocating oil cylinder penetrates through the movable plate to the left, and a connecting rod is hinged to the extending end. The other end of the connecting rod is hinged to the straight cylinder;

[0025] A main oil cylinder is fixed on the left side wall of the straight cylinder. A lifting plate is fixed on the piston rod of the main oil cylinder. A secondary oil cylinder is fixed on the top surface of the lifting plate. The piston rod of the secondary oil cylinder penetrates through the lifting plate to the bottom, and a rack is fixed on the extending end. A connecting plate is fixed on the bottom surface of the lifting plate. A rotating shaft is rotatably installed inside the lower end of the connecting plate. A gear is installed at the left end of the rotating shaft. The gear meshes with the rack. A biofilm installation assembly is arranged directly below the straight cylinder at the right end of the rotating shaft. The biofilm installation assembly includes a lower annular clamping plate, a biofilm, and an upper annular clamping plate stacked in sequence from bottom to top. A plurality of locking screws are connected to the outer edges of the upper annular clamping plate and the lower annular clamping plate. Under the screwing force of the locking screws, the outer edge of the biofilm is clamped and fixed between the upper annular clamping plate and the lower annular clamping plate. The top surface of the upper annular clamping plate presses against a sealing ring on the bottom surface of the straight cylinder. The left side wall of the lower annular clamping plate is welded to the right end of the rotating shaft;

[0026] A fixed seat is fixed on the right side wall of the straight cylinder. A hydraulic motor is fixed on the top surface of the fixed seat. The rotating shaft of the hydraulic motor penetrates through the fixed seat downward, and a vertical oil cylinder is fixed on the extending end. A lifting plate extending to the left is fixed on the acting end of the piston rod of the vertical oil cylinder. A plug is fixed on the top surface of the lifting plate. The plug seals the central hole of the lower annular clamping plate and supports the bottom surface of the biofilm.

[0027] The drive assembly includes a drive motor fixed on the machine table. A vertically arranged jacking oil cylinder is fixed on the output shaft of the drive motor. The L-plate is fixed on the acting end of the piston rod of the jacking oil cylinder.

[0028] A liquid level sensor is fixed on the left side wall inside the straight cylinder.

[0029] Central holes are formed in the middle of the upper annular clamping plate and the lower annular clamping plate of the biofilm installation assembly. The biofilm separates the upper annular clamping plate from the lower annular clamping plate.

[0030] The liquid adding and light shielding assembly includes a bracket fixed on the machine table. A lysis reagent storage tank, a stabilizer storage tank, and a luciferase reagent storage tank are fixed on the cross plate of the bracket. Solenoid valves are connected to the bottom ports of the lysis reagent storage tank, the stabilizer storage tank, and the luciferase reagent storage tank. A light shielding cylinder is also fixed on the cross plate of the bracket. The light shielding cylinder is located on the right side of the luciferase reagent storage tank. The top of the light shielding cylinder is closed, and a photometer is fixed inside the closed end. The photosensitive probe of the photometer is arranged downward.

[0031] A connecting frame is fixed between the closed end of the light shielding cylinder and the cross plate of the bracket.

[0032] A water receiving tank and a waste liquid tank are placed on the tabletop of the machine table. The water receiving tank is located directly below the straight cylinder, and the waste liquid tank is located directly below the light shielding cylinder.

[0033] This high-efficiency detection device further includes a controller. The drive motor, hydraulic motor, lifting oil cylinder, vertical oil cylinder, reciprocating oil cylinder, main oil cylinder, auxiliary oil cylinder, liquid level sensor, and photometer are all electrically connected to the controller via signal lines.

[0034] A high-efficiency detection method for the bacterial content in the pipe string based on a shale gas extraction system includes the following steps:

[0035] S1. Sampling of the sample water, and the specific operation steps are as follows:

[0036] S11. Place the machine table of this detection device on the ground and ensure that the straight cylinder of the detection device is directly below the water outlet pipe of the shale gas extraction system.

[0037] S12. Open the first stop valve. The water in the drainage pipe is branched into the desander through the first stop valve, and then close the first stop valve.

[0038] S13. The desander removes the sand and oil in the water. After standing for 65 minutes, the desander finishes purifying the water. Then open the second stop valve. The purified water in the desander flows through the second stop valve and the water outlet pipe in sequence and finally flows into the straight cylinder. When the water just reaches the liquid level sensor, the liquid level sensor sends an electrical signal to the controller. After the worker receives this electrical signal, the worker immediately closes the second stop valve. At this time, the straight cylinder is filled with sample water, thus completing the sampling of the sample water.

[0039] S2. Separate the bacteria in the sample water, and the specific operation steps are as follows:

[0040] S21. Control the piston rod of the lifting oil cylinder of the auxiliary detection assembly to retract downward. The piston rod drives the L plate to move downward. The L plate drives the feeding oil cylinder, the movable plate, the straight cylinder, and the biofilm installation assembly to move downward synchronously. When the piston rod of the lifting oil cylinder is completely retracted, the straight cylinder moves away from the water outlet pipe and approaches the water receiving tank.

[0041] S22. Control the piston rod of the vertical oil cylinder of the auxiliary detection assembly to extend downward. The piston rod drives the lifting plate to move downward, and the lifting plate drives the plug to move downward. When the piston rod of the vertical oil cylinder is fully extended, the plug just exits from the central hole of the lower annular clamping plate of the biofilm installation assembly.

[0042] S23. Control the rotating shaft of the hydraulic motor of the auxiliary detection assembly to rotate. The rotating shaft drives the vertical oil cylinder, the lifting plate and the plug to rotate synchronously. When the plug rotates 90°, the controller controls the hydraulic motor to close. At this time, the water molecules in the sample water in the straight cylinder sequentially pass through the biofilm and the central hole of the lower annular clamping plate, and finally fall into the water receiving tank. The bacteria in the sample water are intercepted on the top surface of the biofilm because they cannot pass through the biofilm. Thus, the bacteria in the sample water are finally separated. At this time, a layer of bacteria covers the top surface of the biofilm.

[0043] S3. Control the rotating shaft of the hydraulic motor to rotate in the reverse direction so that the plug moves to directly below the central hole of the lower annular clamping plate; then control the piston rod of the vertical oil cylinder to retract upward so that the plug is reinserted into the central hole of the lower annular clamping plate and blocks the biofilm.

[0044] S4. React the bacteria on the biofilm surface into fluorescent substances. The specific operation steps are as follows:

[0045] S41. Control the output shaft of the driving motor to rotate. The output shaft drives the jacking oil cylinder, the L plate, the feeding oil cylinder, the movable plate, the straight cylinder and the biofilm installation assembly to rotate synchronously. When the straight cylinder rotates 180°, the controller controls the driving motor to close. At this time, the straight cylinder just moves to the liquid adding station of the liquid adding and light shielding assembly, that is, directly below the lysis reagent storage tank, the stabilizer storage tank and the luciferase reagent storage tank of the straight cylinder.

[0046] S42. Control the piston rod of the jacking oil cylinder of the auxiliary detection assembly to extend upward. The piston rod drives the L plate to move upward, and the L plate drives the feeding oil cylinder, the movable plate, the straight cylinder and the biofilm installation assembly to move upward synchronously. When the piston rod of the jacking oil cylinder is fully extended, the top port of the straight cylinder is close to the lysis reagent storage tank, the stabilizer storage tank and the luciferase reagent storage tank.

[0047] S43. Control the solenoid valve of the lysis reagent storage tank to start. The lysis reagent in the lysis reagent storage tank enters the straight cylinder through the solenoid valve and submerges the bacteria on the biofilm surface. When the addition reaches the set time point, close the solenoid valve of the lysis reagent storage tank; then control the solenoid valve of the stabilizer storage tank to start. The stabilizer in the stabilizer storage tank enters the straight cylinder through the solenoid valve. When the addition reaches the set time point, close the solenoid valve of the stabilizer storage tank.

[0048] S44. Control the piston rod of the reciprocating oil cylinder of the auxiliary detection component to perform reciprocating telescopic motion. The piston rod drives the connecting rod to perform left and right reciprocating motion, and the connecting rod drives the straight cylinder to perform reciprocating swaying around the hinge seat, thereby mixing the lysis reagent, stabilizer and bacteria. During the mixing process, the lysis reagent destroys the bacteria, so that the ATP inside the bacteria is released, and the stabilizer stabilizes the ATP to prevent ATP degradation; after swaying for 15 minutes, control the reciprocating oil cylinder to close;

[0049] S45. Control the solenoid valve of the luciferase reagent storage tank to start. The luciferase reagent in the luciferase reagent storage tank enters the straight cylinder through the solenoid valve. When the addition reaches the set time point, close the solenoid valve of the luciferase reagent storage tank. At this time, the luciferase reagent reacts with ATP to generate a luminescent fluorescent substance, thereby finally realizing the reaction of all the bacteria on the biofilm surface into fluorescent substances;

[0050] S5. Detection of the light intensity of the light emitted by the fluorescent substance in the straight cylinder. The specific operation steps are as follows:

[0051] S51. Control the piston rod of the lifting oil cylinder of the auxiliary detection component to retract downward. The piston rod drives the L plate to move downward, and the L plate drives the feed oil cylinder, the movable plate, the straight cylinder and the biofilm installation assembly to move downward synchronously;

[0052] S52. Control the piston rod of the feed oil cylinder to extend to the right. The piston rod drives the movable plate to move to the right, and the movable plate drives the straight cylinder and the biofilm installation assembly to move to the right synchronously. When the piston rod of the feed oil cylinder is fully extended, the straight cylinder just moves to directly below the light-shielding cylinder of the liquid addition and light-shielding component;

[0053] S53. Control the piston rod of the lifting oil cylinder to extend upward. The piston rod drives the L plate to move upward, and the L plate drives the straight cylinder to move upward. When the piston rod of the lifting oil cylinder is fully extended, the top port of the straight cylinder just fits into the light-shielding cylinder. At this time, the light-shielding cylinder blocks the top port of the straight cylinder to prevent external natural light from entering the container body. At the same time, the photosensitive probe of the photometer enters the straight cylinder, and the photosensitive probe detects the light intensity of the light emitted by the fluorescent substance. The photosensitive probe converts the light intensity into an electrical signal, and then the photosensitive probe transmits the electrical signal to the controller. The controller calculates the content of bacteria in the pipe column according to the electrical signal;

[0054] When the detected bacteria content is higher than the specified content, it means that the number of bacteria entering the pipe column is large. At this time, the worker increases the displacement of the variable pump, thereby increasing the amount of bactericidal corrosion inhibitor sprayed into the pipe column to avoid the corrosion of the pipe column by bacteria, thereby protecting the pipe column; when the detected bacteria content is lower than the specified content, it means that the number of bacteria entering the pipe column is small. At this time, the worker reduces the displacement of the variable pump, thereby reducing the amount of bactericidal corrosion inhibitor sprayed into the pipe column, thereby saving the usage amount of the bactericidal corrosion inhibitor;

[0055] S6. After the detection is completed, the operation steps for the worker to pour the fluorescent substance and the residual reagent in the straight tube into the waste liquid tank are as follows:

[0056] S61. Control the piston rod of the lifting oil cylinder of the auxiliary detection component to retract downward, so that the straight tube and the biofilm installation assembly move downward synchronously;

[0057] S62. Control the piston rod of the vertical oil cylinder of the auxiliary detection component to extend downward so that the plug just exits from the central hole of the lower annular clamping plate of the biofilm installation assembly; then control the hydraulic motor to rotate. The hydraulic motor drives the vertical oil cylinder, the lifting plate and the plug to rotate synchronously. When the plug rotates 180°, the controller controls the hydraulic motor to shut down. At this time, the residual reagent in the straight tube sequentially passes through the central hole of the upper annular clamping plate, the biofilm, and the central hole of the lower annular clamping plate, and finally falls into the waste liquid tank, thus realizing the discharge of the residual reagent into the waste liquid tank;

[0058] S63. Control the piston rod of the main oil cylinder of the auxiliary detection component to extend downward. The piston rod drives the lifting plate to move downward, and the lifting plate drives the auxiliary oil cylinder, the rack, the connecting plate, the gear, the rotating shaft and the biofilm installation assembly to move downward synchronously. When the piston rod of the main oil cylinder is fully extended, the biofilm installation assembly just separates from the straight tube;

[0059] S64. Control the piston rod of the auxiliary oil cylinder of the auxiliary detection component to retract upward. The piston rod drives the rack to move upward, the rack drives the gear to rotate, the gear drives the rotating shaft to rotate, and the rotating shaft drives the biofilm installation assembly to rotate synchronously. When the piston rod of the auxiliary oil cylinder is fully retracted, the biofilm installation assembly changes to a vertical state. At this time, the fluorescent substance on the surface of the biofilm falls into the waste liquid tank through the central hole of the upper annular clamping plate, thus pouring the fluorescent substance into the waste liquid tank to prepare for the second bacterial content.

[0060] The present invention has the following advantages: reducing the working intensity of workers, greatly improving the detection efficiency of the bacterial content in the pipe string, and being able to timely change the displacement of the variable pump. Brief Description of the Drawings

[0061] Figure 1 It is a schematic structural diagram of a shale gas extraction system;

[0062] Figure 2 It is a schematic diagram of a worker placing a sampling container directly below the water outlet pipe of the shale gas extraction system;

[0063] Figure 3 It is a schematic structural diagram of a filtering container;

[0064] Figure 4 It is a schematic diagram of a worker pouring the sample water in the sampling container into the container body of the filtering container;

[0065] Figure 5 Schematic diagram of a worker embedding a plug into a through groove of an end cover;

[0066] Figure 6 Schematic diagram of a worker sequentially adding a lysis reagent and a stabilizer into the container body of a filtration container;

[0067] Figure 7 Schematic diagram of a worker covering the top port of the container body with a light-shielding cover plate;

[0068] Figure 8 Schematic diagram of a worker inserting a photosensitive probe of a photometer into the light-shielding cover plate;

[0069] Figure 9 Schematic structural diagram of the present invention;

[0070] Figure 10 Schematic structural diagram of an auxiliary detection component;

[0071] Figure 11 For Figure 10 Schematic diagram in the A direction of;

[0072] Figure 12 For Figure 10 Main sectional view of;

[0073] Figure 13 For Figure 12 Connection schematic diagram of the biofilm installation assembly, rotating shaft and gear in;

[0074] Figure 14 For Figure 13 Top view of;

[0075] Figure 15 Schematic structural diagram of a liquid adding and light-shielding component;

[0076] Figure 16 Schematic diagram of the straight cylinder of the present invention being directly below the water outlet pipe of a shale gas extraction system;

[0077] Figure 17 Schematic diagram of the straight cylinder being away from the water outlet pipe;

[0078] Figure 18 Schematic diagram of a plug exiting from the central hole of the lower annular clamping plate of the biofilm installation assembly;

[0079] Figure 19 Schematic diagram of a plug rotating 90°;

[0080] Figure 20 Schematic diagram of the straight cylinder moving to the liquid adding station of the liquid adding and light-shielding component;

[0081] Figure 21Schematic diagram of the top port of the straight tube close to the lysis reagent storage tank, stabilizer storage tank and luciferase reagent storage tank;

[0082] Figure 22 Schematic diagram of the straight tube moving to directly below the light-shielding tube of the liquid addition and light-shielding assembly;

[0083] Figure 23 Schematic diagram of the top port of the straight tube being inserted into the light-shielding tube;

[0084] Figure 24 Schematic diagram of the plug rotating 180°;

[0085] Figure 25 Schematic diagram of the biofilm installation assembly being separated from the straight tube;

[0086] Figure 26 Schematic diagram of the biofilm installation assembly being transformed into a vertical state;

[0087] In the figure:

[0088] 1 - Wellbore, 2 - Christmas tree, 3 - Tubing string, 4 - End valve, 5 - Gas-liquid separator, 6 - Gas transmission pipeline, 7 - Drainage pipeline, 8 - First stop valve, 9 - Desander, 10 - Second stop valve, 11 - Outlet pipe, 12 - Variable pump, 13 - Bactericide and corrosion inhibitor storage tank, 14 - Elbow, 15 - Atomizing ball head; 16 - Sampling container, 17 - Container body, 18 - End cover, 19 - Biofilm, 20 - Through groove, 21 - Plugging head, 22 - Light-shielding cover plate, 23 - Photometer, 24 - Photosensitive probe;

[0089] 25 - Machine platform, 26 - Auxiliary detection assembly, 27 - Liquid addition and light-shielding assembly, 28 - L-shaped plate, 29 - Feed oil cylinder, 30 - Movable plate, 31 - Hinge seat, 32 - Straight tube, 33 - Reciprocating oil cylinder, 34 - Connecting rod;

[0090] 35 - Main oil cylinder, 36 - Lifting plate, 37 - Sub oil cylinder, 38 - Rack, 39 - Connecting plate, 40 - Rotating shaft, 41 - Gear; 42 - Lower annular clamping plate, 43 - Upper annular clamping plate, 44 - Hydraulic motor, 45 - Vertical oil cylinder, 46 - Lifting plate, 47 - Plug, 48 - Driving motor, 49 - Jacking oil cylinder;

[0091] 50 - Bracket, 51 - Lysis reagent storage tank, 52 - Stabilizer storage tank, 53 - Luciferase reagent storage tank, 54 - Light-shielding tube; 55 - Water receiving tank, 56 - Waste liquid tank. Detailed implementation manners

[0092] The following further describes the present invention in conjunction with the accompanying drawings. The protection scope of the present invention is not limited to the following:

[0093] As Figure 9As shown in the figure, an efficient detection device for the bacterial content in the pipe string of a shale gas extraction system includes a machine platform 25 supported on the ground, and an auxiliary detection component 26 arranged on the machine platform 25 for collecting sample water, automatically separating bacteria in the sample water alone, and automatically shaking reagents. A liquid adding and light shielding component 27 is also arranged on the machine platform 25 on the right side of the auxiliary detection component 26.

[0094] As Figures 10 to 14 shown in the figure, the auxiliary detection component 26 includes a driving assembly fixed on the machine platform 25. The top of the driving assembly is fixedly provided with an L-shaped plate 28. A feeding oil cylinder 29 is fixedly arranged on the L-shaped plate 28. The piston rod of the feeding oil cylinder 29 penetrates through the L-shaped plate 28 to the left, and a movable plate 30 is fixedly arranged on the extending end. A straight cylinder 32 is hinged to the left side of the movable plate 30 through a hinge seat 31. A reciprocating oil cylinder 33 is fixedly arranged on the right side of the movable plate 30. The piston rod of the reciprocating oil cylinder 33 penetrates through the movable plate 30 to the left, and a connecting rod 34 is hinged to the extending end. The other end of the connecting rod 34 is hinged to the straight cylinder 32. A liquid level sensor is fixedly arranged on the left side wall inside the straight cylinder 32. The driving assembly includes a driving motor 48 fixed on the machine platform 25. A vertically arranged jacking oil cylinder 49 is fixedly arranged on the output shaft of the driving motor 48. The L-shaped plate 28 is fixedly arranged on the acting end of the piston rod of the jacking oil cylinder 49.

[0095] As Figures 10 to 14 shown in the figure, a main oil cylinder 35 is fixedly arranged on the left side wall of the straight cylinder 32. A lifting plate 36 is fixedly arranged on the piston rod of the main oil cylinder 35. A secondary oil cylinder 37 is fixedly arranged on the top surface of the lifting plate 36. The piston rod of the secondary oil cylinder 37 penetrates through the lifting plate 36 to the bottom, and a rack 38 is fixedly arranged on the extending end. A connecting plate 39 is fixedly arranged on the bottom surface of the lifting plate 36. A rotating shaft 40 is rotatably installed inside the lower end of the connecting plate 39. A gear 41 is installed at the left end of the rotating shaft 40. The gear 41 meshes with the rack 38. A biofilm installation assembly is arranged directly below the straight cylinder 32 at the right end of the rotating shaft 40. The biofilm installation assembly includes a lower annular clamping plate 42, a biofilm 19, and an upper annular clamping plate 43 stacked in sequence from bottom to top. A plurality of locking screws are connected to the outer edges of the upper annular clamping plate 43 and the lower annular clamping plate 42. Under the screwing force of the locking screws, the outer edge of the biofilm 19 is clamped and fixed between the upper annular clamping plate 43 and the lower annular clamping plate 42. The top surface of the upper annular clamping plate 43 presses against the sealing ring on the bottom surface of the straight cylinder 32. The left side wall of the lower annular clamping plate 42 is welded to the right end of the rotating shaft 40. Central holes are opened in the middle of the upper annular clamping plate 43 and the lower annular clamping plate 42 of the biofilm installation assembly. The biofilm 19 separates the upper annular clamping plate 43 from the lower annular clamping plate 42.

[0096] A fixing seat is fixedly arranged on the right side wall of the straight cylinder 32. A hydraulic motor 44 is fixedly arranged on the top surface of the fixing seat. The rotating shaft of the hydraulic motor 44 penetrates downward through the fixing seat, and a vertical oil cylinder 45 is fixedly arranged on the extending end. A lifting plate 46 extending leftward is fixedly arranged on the acting end of the piston rod of the vertical oil cylinder 45. A plug 47 is fixedly arranged on the top surface of the lifting plate 46. The plug 47 blocks the central hole of the lower annular clamping plate 42 and supports the bottom surface of the biofilm 19.

[0097] As Figure 15 shown, the liquid adding and light shielding assembly 27 includes a bracket 50 fixedly arranged on the machine table 25. A lysis reagent storage tank 51, a stabilizer storage tank 52 and a luciferase reagent storage tank 53 are fixedly arranged on the cross plate of the bracket 50. Solenoid valves are connected to the bottom ports of the lysis reagent storage tank 51, the stabilizer storage tank 52 and the luciferase reagent storage tank 53. A light shielding cylinder 54 is also fixedly arranged on the cross plate of the bracket 50. The light shielding cylinder 54 is located on the right side of the luciferase reagent storage tank 53. The top of the light shielding cylinder 54 is closed, and a photometer 23 is fixedly arranged inside the closed end. The photosensitive probe 24 of the photometer 23 is arranged downward. A connecting frame is fixedly arranged between the closed end of the light shielding cylinder 54 and the cross plate of the bracket 50.

[0098] A water receiving tank 55 and a waste liquid tank 56 are placed on the table top of the machine table 25. The water receiving tank 55 is located directly below the straight cylinder 32, and the waste liquid tank 56 is located directly below the light shielding cylinder 54.

[0099] This high-efficiency detection device further includes a controller. The drive motor 48, the hydraulic motor 44, the jacking oil cylinder 49, the vertical oil cylinder 45, the reciprocating oil cylinder 33, the main oil cylinder 35, the auxiliary oil cylinder 37, the liquid level sensor and the photometer 23 are all electrically connected to the controller through signal lines. Workers can control the start or stop of the drive motor 48 and the hydraulic motor 44 through the controller. At the same time, they can also control the extension or retraction of the piston rods of the jacking oil cylinder 49, the vertical oil cylinder 45, the reciprocating oil cylinder 33, the main oil cylinder 35 and the auxiliary oil cylinder 37, thus facilitating the operation of workers and having the characteristics of high automation.

[0100] A high-efficiency detection method for the bacterial content in the pipe string of a shale gas exploitation system includes the following steps:

[0101] S1. Sampling of the sample water, and its specific operation steps are as follows:

[0102] S11. Place the machine table 25 of this detection device on the ground and ensure that the straight cylinder 32 of the detection device is directly below the water outlet pipe 11 of the shale gas exploitation system as Figure 1 shown, as Figure 16 shown;

[0103] S12. Open the first stop valve 8, and the water in the drainage pipe 7 is diverted through the first stop valve 8 into the desander 9, and then close the first stop valve 8;

[0104] S13. The desander 9 removes sand and oil from the water. After standing for 65 minutes, the desander 9 finishes purifying the water. Then open the second stop valve 10, and the purified water in the desander 9 sequentially passes through the second stop valve 10 and the outlet pipe 11, and finally flows into the straight cylinder 32. When the water just reaches the liquid level sensor, the liquid level sensor sends an electrical signal to the controller. After the worker receives this electrical signal, the worker immediately closes the second stop valve 10. At this time, the straight cylinder 32 contains the sample water, thus completing the sampling of the sample water;

[0105] S2. Separate the bacteria in the sample water, and the specific operation steps are as follows:

[0106] S21. Control the piston rod of the lifting oil cylinder 49 of the auxiliary detection assembly 26 to retract downward. The piston rod drives the L plate 28 to move downward, and the L plate 28 drives the feeding oil cylinder 29, the movable plate 30, the straight cylinder 32 and the biofilm installation assembly to move downward synchronously. When the piston rod of the lifting oil cylinder 49 is fully retracted, the straight cylinder 32 moves away from the outlet pipe 11, as Figure 17 shown, and is close to the water receiving tank 55;

[0107] S22. Control the piston rod of the vertical oil cylinder 45 of the auxiliary detection assembly 26 to extend downward. The piston rod drives the lifting plate 46 to move downward, and the lifting plate 46 drives the plug 47 to move downward. When the piston rod of the vertical oil cylinder 45 is fully extended, the plug 47 just withdraws from the central hole of the lower annular clamping plate 42 of the biofilm installation assembly, as Figure 18 shown;

[0108] S23. Control the rotating shaft of the hydraulic motor 44 of the auxiliary detection assembly 26 to rotate. The rotating shaft drives the vertical oil cylinder 45, the lifting plate 46 and the plug 47 to rotate synchronously. When the plug 47 rotates 90°, as Figure 19 shown, the controller controls the hydraulic motor 44 to close. At this time, the water molecules in the sample water in the straight cylinder 32 sequentially pass through the biofilm 19 and the central hole of the lower annular clamping plate 42, and finally fall into the water receiving tank 55. The movement direction of the water molecules is as Figure 19 shown by the hollow arrow in the figure, while the bacteria in the sample water are intercepted on the top surface of the biofilm 19 because they cannot pass through the biofilm 19. Thus, the separation of the bacteria in the sample water is finally realized. At this time, a layer of bacteria covers the top surface of the biofilm 19;

[0109] S3. Control the reverse rotation of the rotating shaft of the hydraulic motor 44 so that the plug 47 moves to directly below the central hole of the lower annular clamping plate 42; then control the piston rod of the vertical cylinder 45 to retract upward so that the plug 47 is reinserted into the central hole of the lower annular clamping plate 42 and blocks the biofilm 19.

[0110] S4. React the bacteria on the surface of the biofilm 19 into fluorescent substances. The specific operation steps are as follows:

[0111] S41. Control the output shaft of the drive motor 48 to rotate. The output shaft drives the lifting cylinder 49, the L-shaped plate 28, the feeding cylinder 29, the movable plate 30, the straight cylinder 32 and the biofilm installation assembly to rotate synchronously. When the straight cylinder 32 rotates 180°, the controller controls the drive motor 48 to turn off. At this time, the straight cylinder 32 just moves to the liquid addition station of the liquid addition and light shielding assembly 27, as Figure 20 shown, that is, directly below the lysis reagent storage tank 51, the stabilizer storage tank 52 and the luciferase reagent storage tank 53 of the straight cylinder 32.

[0112] S42. Control the piston rod of the lifting cylinder 49 of the auxiliary detection assembly 26 to extend upward. The piston rod drives the L-shaped plate 28 to move upward. The L-shaped plate 28 drives the feeding cylinder 29, the movable plate 30, the straight cylinder 32 and the biofilm installation assembly to move upward synchronously. When the piston rod of the lifting cylinder 49 is fully extended, the top port of the straight cylinder 32 is close to the lysis reagent storage tank 51, the stabilizer storage tank 52 and the luciferase reagent storage tank 53, as Figure 21 shown.

[0113] S43. Control the solenoid valve of the lysis reagent storage tank 51 to start. The lysis reagent in the lysis reagent storage tank 51 enters the straight cylinder 32 through the solenoid valve and submerges the bacteria on the surface of the biofilm 19. When the addition reaches the set time point, close the solenoid valve of the lysis reagent storage tank 51; then control the solenoid valve of the stabilizer storage tank 52 to start. The stabilizer in the stabilizer storage tank 52 enters the straight cylinder 32 through the solenoid valve. When the addition reaches the set time point, close the solenoid valve of the stabilizer storage tank 52.

[0114] S44. Control the piston rod of the reciprocating cylinder 33 of the auxiliary detection assembly 26 to perform reciprocating telescopic motion. The piston rod drives the connecting rod 34 to move left and right reciprocally. The connecting rod 34 drives the straight cylinder 32 to reciprocally shake around the hinge seat 31. The shaking direction of the straight cylinder 32 is as shown by the arrow in Figure 21 . Thus, the lysis reagent, the stabilizer and the bacteria are mixed. During the mixing process, the lysis reagent destroys the bacteria so that the ATP in the bacteria is released, and the stabilizer stabilizes the ATP to prevent ATP degradation; when shaking for 15 minutes, control the reciprocating cylinder 33 to close.

[0115] S45. Control the solenoid valve of the luciferase reagent storage tank 53 to start. The luciferase reagent in the luciferase reagent storage tank 53 enters the straight tube 32 through the solenoid valve. When the addition reaches the set time point, close the solenoid valve of the luciferase reagent storage tank 53. At this time, the luciferase reagent reacts with ATP to generate a luminescent fluorescent substance, thus finally realizing the reaction of all bacteria on the surface of the biofilm 19 into fluorescent substances;

[0116] S5. Detection of the light intensity of the light emitted by the fluorescent substance in the straight tube 32. The specific operation steps are as follows:

[0117] S51. Control the piston rod of the lifting oil cylinder 49 of the auxiliary detection assembly 26 to retract downward. The piston rod drives the L plate 28 to move downward, and the L plate 28 drives the feed oil cylinder 29, the movable plate 30, the straight tube 32 and the biofilm installation assembly to move downward synchronously;

[0118] S52. Control the piston rod of the feed oil cylinder 29 to extend to the right. The piston rod drives the movable plate 30 to move to the right, and the movable plate 30 drives the straight tube 32 and the biofilm installation assembly to move to the right synchronously. When the piston rod of the feed oil cylinder 29 is fully extended, the straight tube 32 just moves to directly below the light-shielding tube 54 of the liquid addition and light-shielding assembly 27, as Figure 22 shown;

[0119] S53. Control the piston rod of the lifting oil cylinder 49 to extend upward. The piston rod drives the L plate 28 to move upward, and the L plate 28 drives the straight tube 32 to move upward. When the piston rod of the lifting oil cylinder 49 is fully extended, the top port of the straight tube 32 just fits into the light-shielding tube 54, as Figure 23 shown. At this time, the light-shielding tube 54 blocks the top port of the straight tube 32 to prevent external natural light from entering the container body 17. At the same time, the photosensitive probe 24 of the photometer 23 enters the straight tube 32. The photosensitive probe 24 detects the light intensity of the fluorescent substance, converts the light intensity into an electrical signal, and then the photosensitive probe 24 transmits the electrical signal to the controller. The controller calculates the content of bacteria in the pipe column 3 according to the electrical signal;

[0120] When the detected bacteria content is higher than the specified content, it indicates that the number of bacteria entering the pipe column 3 is large. At this time, the worker increases the displacement of the variable pump 12, thereby increasing the amount of bactericidal corrosion inhibitor sprayed into the pipe column 3 to avoid the corrosion of the pipe column 3 by bacteria and thus protect the pipe column 3; when the detected bacteria content is lower than the specified content, it indicates that the number of bacteria entering the pipe column 3 is small. At this time, the worker reduces the displacement of the variable pump 12, thereby reducing the amount of bactericidal corrosion inhibitor sprayed into the pipe column 3, thus saving the usage amount of the bactericidal corrosion inhibitor.

[0121] Among them, in step S2, this detection device can withdraw the blockage 47 from the central hole of the lower annular clamping plate 42 of the biofilm installation assembly only through the cooperation of the vertical oil cylinder 45 and the hydraulic motor 44 of the auxiliary detection assembly 26, and then automatically separate the bacteria in the sample water; in step S4, this detection device can automatically add lysis reagent, stabilizer and luciferase reagent into the straight cylinder 32 and automatically shake the straight cylinder 32 only through the linkage cooperation of the auxiliary detection assembly 26 and the liquid addition and light shielding assembly 27, so that the lysis reagent, stabilizer and bacteria are mixed, and then the ATP in the bacteria is released; in step S5, this detection device can automatically put the light shielding cylinder 54 on the top port of the straight cylinder 32 and make the photosensitive probe 24 of the photometer 23 extend into the straight cylinder 32 only through the linkage cooperation of the auxiliary detection assembly 26 and the liquid addition and light shielding assembly 27, so as to detect the light intensity, and then detect the content of bacteria in the pipe column 3.

[0122] It can be seen from this that compared with the detection method as Figures 2 to 8 shown, this detection device does not require workers to manually pour the sample water in the sampling container 16 into the container body 17 of the filtering container to separately separate the bacteria in the sample water; nor does it require workers to shake the container body 17 to mix the lysis reagent, stabilizer and bacteria; nor does it require workers to sequentially add lysis reagent, stabilizer and luciferase reagent into the container body 17 of the filtering container to react to generate fluorescent substances; nor does it require workers to cover the top port of the container body 17 of the filtering container with the light shielding cover plate 22, nor does it require workers to extend the photosensitive probe 24 of the photometer 23 into the light shielding cover plate 22 to detect the light intensity with the photometer 23.

[0123] This detection device realizes automatic and continuous detection, which not only greatly reduces the working intensity of workers, but also realizes the detection of the content of bacteria in a short time, thus greatly improving the detection efficiency of the content of bacteria in the pipe column 3, so as to timely change the displacement of the variable pump 12, thereby protecting the pipe column 3 and saving the usage amount of the bactericidal corrosion inhibitor.

[0124] S6. After the detection is completed, the operation steps for the worker to pour the fluorescent substances and residual reagents in the straight cylinder 32 into the waste liquid tank 56 are as follows:

[0125] S61. Control the piston rod of the jacking oil cylinder 49 of the auxiliary detection assembly 26 to retract downward, so that the straight cylinder 32 and the biofilm installation assembly move downward synchronously.

[0126] S62. Control the piston rod of the vertical oil cylinder 45 of the auxiliary detection assembly 26 to extend downward so that the plug 47 just exits from the central hole of the lower annular clamping plate 42 of the biofilm installation assembly; then control the hydraulic motor 44 to rotate. The hydraulic motor 44 drives the vertical oil cylinder 45, the lifting plate 46 and the plug 47 to rotate synchronously. When the plug 47 rotates 180°, as Figure 24 shown, the controller controls the hydraulic motor 44 to shut down. At this time, the residual reagent in the straight cylinder 32 sequentially passes through the central hole of the upper annular clamping plate 43, the biofilm 19, and the central hole of the lower annular clamping plate 42, and finally falls into the waste liquid tank 56. The moving direction of the residual reagent is as Figure 24 shown by the arrow in, thus realizing the discharge of the residual reagent into the waste liquid tank 56;

[0127] S63. Control the piston rod of the main oil cylinder 35 of the auxiliary detection assembly 26 to extend downward. The piston rod drives the lifting plate 36 to move downward. The lifting plate 36 drives the auxiliary oil cylinder 37, the rack 38, the connecting plate 39, the gear 41, the rotating shaft 40 and the biofilm installation assembly to move downward synchronously. When the piston rod of the main oil cylinder 35 is fully extended, the biofilm installation assembly just separates from the straight cylinder 32, as Figure 25 shown;

[0128] S64. Control the piston rod of the auxiliary oil cylinder 37 of the auxiliary detection assembly 26 to retract upward. The piston rod drives the rack 38 to move upward. The rack 38 drives the gear 41 to rotate. The gear 41 drives the rotating shaft 40 to rotate. The rotating shaft 40 drives the biofilm installation assembly to rotate synchronously. When the piston rod of the auxiliary oil cylinder 37 is fully retracted, the biofilm installation assembly changes to a vertical state, as Figure 26 shown. At this time, the fluorescent substances on the surface of the biofilm 19 fall into the waste liquid tank 56 through the central hole of the upper annular clamping plate 43, thereby pouring the fluorescent substances into the waste liquid tank 56 to prepare for the second bacterial content.

[0129] Among them, as can be seen from step S6, this detection device only needs to first control the piston rod of the vertical oil cylinder 45 to extend downward so that the plug 47 just exits from the central hole of the lower annular clamping plate 42 of the biofilm installation assembly, thereby realizing the discharge of the residual reagent in the straight cylinder 32 into the waste liquid tank 56; then control the linkage cooperation of the main oil cylinder 35 and the auxiliary oil cylinder 37 to change the biofilm installation assembly to a vertical state, thereby realizing the pouring of the fluorescent substances on the surface of the biofilm 19 into the waste liquid tank 56. It can be seen from this that this detection device realizes the automatic pouring of all the fluorescent substances and residual reagents in the straight cylinder 32 into the waste liquid tank 56 without manual pouring, thus greatly reducing the working intensity of the workers.

Claims

1. An efficient detection device for bacterial content in a pipe column of a shale gas production system, characterized in that: The device comprises a machine platform (25) supported on the ground, an auxiliary detection component (26) arranged on the machine platform (25) for collecting sample water, automatically separating bacteria in the sample water, and automatically shaking the reagent, and a liquid adding and light shielding component (27) located on the right side of the auxiliary detection component (26) is also arranged on the machine platform (25); The auxiliary detection component (26) comprises a driving assembly fixedly mounted on the machine platform (25), an L-plate (28) fixedly mounted on the top of the driving assembly, a feed cylinder (29) fixedly mounted on the L-plate (28), a piston rod of the feed cylinder (29) passing through the L-plate (28) to the left and a movable plate (30) fixedly mounted on the extended end, a straight cylinder (32) hingedly mounted on the left side of the movable plate (30) via a hinge seat (31), a reciprocating cylinder (33) fixedly mounted on the right side of the movable plate (30), a piston rod of the reciprocating cylinder (33) passing through the movable plate (30) to the left and a connecting rod (34) hingedly mounted on the extended end, and the other end of the connecting rod (34) hingedly mounted on the straight cylinder (32); A main oil cylinder (35) is fixedly provided on the left side wall of the straight cylinder (32); a lifting plate (36) is fixedly provided on the piston rod of the main oil cylinder (35); a sub-oil cylinder (37) is fixedly provided on the top surface of the lifting plate (36); a piston rod of the sub-oil cylinder (37) passes through the lifting plate (36) downward and a rack (38) is fixedly provided on the extended end; a connecting plate (39) is fixedly provided on the bottom surface of the lifting plate (36); a rotating shaft (40) is rotatably installed in the lower end of the connecting plate (39); a gear (41) is installed on the left end of the rotating shaft (40); the gear (41) is meshed with the rack (38); a gear (41) is provided on the right end of the rotating shaft (40) and is located in the straight cylinder ( 32), the biofilm installation assembly comprising a lower annular clamping plate (42), a biofilm (19) and an upper annular clamping plate (43) stacked in sequence from bottom to top, a plurality of locking screws being connected at the outer edges of the upper annular clamping plate (43) and the lower annular clamping plate (42), under the threaded connection force of the locking screws, the outer edge of the biofilm (19) is clamped and fixed between the upper annular clamping plate (43) and the lower annular clamping plate (42), the top surface of the upper annular clamping plate (43) is pressed against the sealing ring located on the bottom surface of the straight cylinder (32), and the left side wall of the lower annular clamping plate (42) is welded to the right end of the rotating shaft (40); A fixing seat is fixedly provided on the right side wall of the straight cylinder (32), a hydraulic motor (44) is fixedly provided on the top surface of the fixing seat, a rotating shaft of the hydraulic motor (44) passes downward through the fixing seat, and a vertical oil cylinder (45) is fixedly provided on the extended end, a lifting plate (46) extending to the left is fixedly provided on the action end of the piston rod of the vertical oil cylinder (45), a plug (47) is fixedly provided on the top surface of the lifting plate (46), and the plug (47) blocks the central hole of the lower annular clamping plate (42) and supports the bottom surface of the biofilm (19); The driving assembly comprises a driving motor (48) fixedly mounted on the machine platform (25); a vertically mounted lifting cylinder (49) is fixedly mounted on the output shaft of the driving motor (48); and the L-plate (28) is fixedly mounted on the action end of the piston rod of the lifting cylinder (49); The liquid adding and light shielding component (27) comprises a bracket (50) fixedly mounted on the machine platform (25); a lysis reagent storage tank (51), a stabilizer storage tank (52) and a luciferase reagent storage tank (53) are fixedly mounted on a horizontal plate of the bracket (50); and the bottom ports of the lysis reagent storage tank (51), the stabilizer storage tank (52) and the luciferase reagent storage tank (53) are all connected to electromagnetic valves; a light shielding tube (54) is also fixedly mounted on the horizontal plate of the bracket (50); the light shielding tube (54) is located on the right side of the luciferase reagent storage tank (53); the top of the light shielding tube (54) is closed, and a photometer (23) is fixedly mounted in the closed end; and the photosensitive probe (24) of the photometer (23) is arranged downward.

2. According to claim 1, a highly efficient detection device for bacteria content in a pipe column of a shale gas production system is characterized by: A liquid level sensor is fixedly arranged in the straight cylinder (32) and on its left side wall.

3. The high-efficiency detection device for bacteria content in a pipe column based on a shale gas production system according to claim 2, characterized in that: The upper annular clamping plate (43) and the lower annular clamping plate (42) of the biofilm installation assembly are both provided with a central hole in the middle, and the biofilm (19) separates the upper annular clamping plate (43) from the lower annular clamping plate (42).

4. The high-efficiency detection device for bacteria content in a pipe column based on a shale gas production system according to claim 3 is characterized by: A connecting frame is fixedly provided between the closed end of the light-shielding tube (54) and the horizontal plate of the bracket (50).

5. The high-efficiency detection device for bacteria content in a pipe column based on a shale gas production system according to claim 4 is characterized in that: A water receiving trough (55) and a waste liquid trough (56) are placed on the table top of the machine table (25); the water receiving trough (55) is located directly below the straight tube (32), and the waste liquid trough (56) is located directly below the light shielding tube (54).

6. The high-efficiency detection device for bacteria content in a pipe column based on a shale gas production system according to claim 5, characterized in that: The high-efficiency detection device also includes a controller, wherein the drive motor (48), the hydraulic motor (44), the lifting cylinder (49), the vertical cylinder (45), the reciprocating cylinder (33), the main cylinder (35), the auxiliary cylinder (37), the liquid level sensor and the photometer (23) are all electrically connected to the controller via signal lines.

7. A method for efficiently detecting the bacterial content in a pipe column of a shale gas production system, using a device for efficiently detecting the bacterial content in a pipe column of a shale gas production system as claimed in claim 6, wherein the shale gas production system comprises a wellbore (1) fixed in a well, a gas production tree (2) fixed on the top surface of the wellbore (1), a pipe column (3) is arranged in the wellbore (1), the top end of the pipe column (3) is fixed on the gas production tree (2), a gas-water separator (5) is connected to the end valve (4) of the gas production tree (2), a gas transmission pipeline (6) and a drainage pipeline (7) are connected to the gas-water separator (5), a first stop valve (8) is connected to the drainage pipeline (7), and a sand removal device is connected to the end of the first stop valve (8). The desander (9) is connected to a second stop valve (10) at the end port of the desander (9), and a water outlet pipe (11) is connected to the other end port of the second stop valve (10). In an initial state, the first stop valve (8) and the second stop valve (10) are both in a closed state. The shale gas extraction system further comprises a variable pump (12) and a bactericidal corrosion inhibitor storage tank (13) arranged on the ground, a liquid extraction port of the variable pump (12) is connected to the bactericidal corrosion inhibitor storage tank (13), and a bent pipe (14) is connected to the liquid discharge port of the variable pump (12), the other end of the bent pipe (14) penetrates the side wall of the wellbore (1) and extends directly below the pipe string (3), and an atomizing ball head (15) is connected to the extended end of the bent pipe (14), characterized in that: It includes the following steps: S1. Sampling of sample water. The specific operation steps are as follows: S11, placing the platform (25) of the detection device on the ground, and ensuring that the straight cylinder (32) of the detection device is directly below the water outlet pipe (11) of the shale gas extraction system; S12, opening the first stop valve (8), so that the water in the drainage pipe (7) is diverted to the desander (9) through the first stop valve (8), and then the first stop valve (8) is closed; S13, the desander (9) removes sand and oil from the water. After standing still for 65 minutes, the desander (9) has completely purified the water. Then, the second stop valve (10) is opened, and the purified water in the desander (9) passes through the second stop valve (10) and the water outlet pipe (11) in sequence, and finally flows into the straight cylinder (32). When the water just reaches the liquid level sensor, the liquid level sensor sends an electrical signal to the controller. After the worker receives the electrical signal, the worker immediately closes the second stop valve (10). At this time, the straight cylinder (32) is filled with sample water, thereby completing the sampling of the sample water. S2. Separate the bacteria from the sample water. The specific steps are as follows: S21, the piston rod of the lifting cylinder (49) of the auxiliary detection component (26) is controlled to retract downward, the piston rod drives the L plate (28) to move downward, and the L plate (28) drives the feed cylinder (29), the movable plate (30), the straight cylinder (32) and the biofilm installation assembly to move downward synchronously. When the piston rod of the lifting cylinder (49) is fully retracted, the straight cylinder (32) is separated from the water outlet pipe (11) and approaches the water receiving tank (55); S22, controlling the piston rod of the vertical oil cylinder (45) of the auxiliary detection component (26) to extend downward, the piston rod drives the lifting plate (46) to move downward, and the lifting plate (46) drives the plug (47) to move downward, and when the piston rod of the vertical oil cylinder (45) is fully extended, the plug (47) just exits from the center hole of the lower annular clamping plate (42) of the biofilm installation assembly; S23, controlling the rotation shaft of the hydraulic motor (44) of the auxiliary detection component (26) to rotate, and the rotation shaft drives the vertical oil cylinder (45), the lifting plate (46) and the plug (47) to rotate synchronously. When the plug (47) rotates 90 degrees, the controller controls the hydraulic motor (44) to close. At this time, the water molecules of the sample water in the straight cylinder (32) pass through the biofilm (19) and the central hole of the lower annular clamping plate (42) in sequence, and finally fall into the water receiving tank (55). The bacteria in the sample water cannot pass through the biofilm (19) and are intercepted on the top surface of the biofilm (19), thereby finally achieving the separation of the bacteria in the sample water. At this time, the top surface of the biofilm (19) is covered with a layer of bacteria; S3, controlling the rotating shaft of the hydraulic motor (44) to rotate in the opposite direction, so that the plug (47) moves to the position directly below the center hole of the lower annular clamp (42); then controlling the piston rod of the vertical oil cylinder (45) to retract upward, so that the plug (47) is inserted back into the center hole of the lower annular clamp (42), thereby blocking the biofilm (19); S4. Reacting the bacteria on the surface of the biofilm (19) into fluorescent substances, the specific operation steps are as follows: S41, controlling the output shaft of the driving motor (48) to rotate, and the output shaft drives the lifting cylinder (49), the L plate (28), the feeding cylinder (29), the movable plate (30), the straight cylinder (32) and the biofilm installation assembly to rotate synchronously. When the straight cylinder (32) rotates 180°, the controller controls the driving motor (48) to turn off. At this time, the straight cylinder (32) just moves to the liquid adding station of the liquid adding and shading component (27), that is, directly below the lysis reagent storage tank (51), the stabilizer storage tank (52) and the luciferase reagent storage tank (53) of the straight cylinder (32); S42, controlling the piston rod of the lifting cylinder (49) of the auxiliary detection assembly (26) to extend upward, the piston rod drives the L plate (28) to move upward, the L plate (28) drives the feed cylinder (29), the movable plate (30), the straight cylinder (32) and the biofilm installation assembly to move upward synchronously, and when the piston rod of the lifting cylinder (49) is fully extended, the top end of the straight cylinder (32) is close to the lysis reagent storage tank (51), the stabilizer storage tank (52) and the luciferase reagent storage tank (53); S43, controlling the solenoid valve of the lysis reagent storage tank (51) to start, the lysis reagent in the lysis reagent storage tank (51) enters into the straight cylinder (32) through the solenoid valve, and submerges the bacteria on the surface of the biofilm (19), and when the bacteria are added to the set time point, the solenoid valve of the lysis reagent storage tank (51) is closed; and then controlling the solenoid valve of the stabilizer storage tank (52) to start, the stabilizer in the stabilizer storage tank (52) enters into the straight cylinder (32) through the solenoid valve, and when the bacteria are added to the set time point, the solenoid valve of the stabilizer storage tank (52) is closed; S44, controlling the piston rod of the reciprocating cylinder (33) of the auxiliary detection component (26) to perform reciprocating telescopic motion, the piston rod drives the connecting rod (34) to perform left and right reciprocating motion, the connecting rod (34) drives the straight cylinder (32) to perform reciprocating shaking around the hinge seat (31), thereby mixing the lysis reagent, the stabilizer and the bacteria. During the mixing process, the lysis reagent destroys the bacteria to release ATP in the bacteria, while the stabilizer stabilizes the ATP to prevent ATP degradation; after shaking for 15 minutes, controlling the reciprocating cylinder (33) to close; S45, controlling the solenoid valve of the luciferase reagent storage tank (53) to start, and the luciferase reagent in the luciferase reagent storage tank (53) enters the straight cylinder (32) through the solenoid valve. When the luciferase reagent is added to the set time point, the solenoid valve of the luciferase reagent storage tank (53) is closed. At this time, the luciferase reagent reacts with ATP to generate a luminescent fluorescent substance, thereby finally achieving the reaction of all bacteria on the surface of the biofilm (19) into fluorescent substances; S5, detecting the light intensity of the light emitted by the fluorescent substance in the straight tube (32), the specific operation steps are as follows: S51, controlling the piston rod of the lifting cylinder (49) of the auxiliary detection component (26) to retract downward, the piston rod drives the L plate (28) to move downward, and the L plate (28) drives the feed cylinder (29), the movable plate (30), the straight cylinder (32) and the biofilm installation assembly to move downward synchronously; S52, the piston rod of the control feed cylinder (29) is extended to the right, the piston rod drives the movable plate (30) to move to the right, the movable plate (30) drives the straight cylinder (32) and the biofilm installation assembly to move to the right synchronously, and when the piston rod of the feed cylinder (29) is fully extended, the straight cylinder (32) moves just below the light shielding cylinder (54) of the liquid adding and light shielding assembly (27); S53, control the piston rod of the lifting cylinder (49) to extend upward, the piston rod drives the L plate (28) to move upward, the L plate (28) drives the straight tube (32) to move upward, when the piston rod of the lifting cylinder (49) is fully extended, the top end of the straight tube (32) is just embedded in the light shielding tube (54), at this time, the light shielding tube (54) shields the top end of the straight tube (32) to prevent external natural light from entering the container body (17), at the same time, the photosensitive probe (24) of the photometer (23) enters the straight tube (32), the photosensitive probe (24) detects the light intensity of the light emitted by the fluorescent object, the photosensitive probe (24) converts the light intensity into an electrical signal, and then the photosensitive probe (24) transmits the electrical signal to the controller, and the controller calculates the content of bacteria in the pipe column (3) according to the electrical signal; When the detected bacterial content is higher than the specified content, it means that the number of bacteria entering the pipe column (3) is large. At this time, the worker increases the displacement of the variable pump (12), thereby increasing the amount of bactericidal corrosion inhibitor sprayed into the pipe column (3) to prevent bacteria from corroding the pipe column (3), thereby protecting the pipe column (3); when the detected bacterial content is lower than the specified content, it means that the number of bacteria entering the pipe column (3) is small. At this time, the worker decreases the displacement of the variable pump (12), thereby reducing the amount of bactericidal corrosion inhibitor sprayed into the pipe column (3), thereby saving the amount of bactericidal corrosion inhibitor used; S6. After the test is completed, the worker dumps the fluorescent substance and residual reagent in the straight cylinder (32) into the waste liquid tank (56). The operation steps are as follows: S61, controlling the piston rod of the lifting cylinder (49) of the auxiliary detection assembly (26) to retract downward, thereby causing the straight cylinder (32) and the biofilm installation assembly to move downward synchronously; S62, controlling the piston rod of the vertical oil cylinder (45) of the auxiliary detection component (26) to extend downward so that the plug (47) just exits from the center hole of the lower annular clamp (42) of the biofilm installation assembly; then controlling the hydraulic motor (44) to rotate, the hydraulic motor (44) drives the vertical oil cylinder (45), the lifting plate (46) and the plug (47) to rotate synchronously, and when the plug (47) rotates 180°, the controller controls the hydraulic motor (44) to turn off, at which time, the residual reagent in the straight cylinder (32) passes through the center hole of the upper annular clamp (43), the biofilm (19), the center hole of the lower annular clamp (42), and finally falls into the waste liquid tank (56), thereby achieving the discharge of the residual reagent into the waste liquid tank (56); S63, the piston rod of the main oil cylinder (35) of the control auxiliary detection component (26) extends downward, the piston rod drives the lifting plate (36) to move downward, the lifting plate (36) drives the auxiliary oil cylinder (37), the rack (38), the connecting plate (39), the gear (41), the rotating shaft (40) and the biofilm installation assembly to move downward synchronously, and when the piston rod of the main oil cylinder (35) is fully extended, the biofilm installation assembly is just separated from the straight cylinder (32); S64, the piston rod of the auxiliary cylinder (37) of the control auxiliary detection component (26) is retracted upward, the piston rod drives the rack (38) to move upward, the rack (38) drives the gear (41) to rotate, the gear (41) drives the shaft (40) to rotate, and the shaft (40) drives the biofilm installation assembly to rotate synchronously. When the piston rod of the auxiliary cylinder (37) is fully retracted, the biofilm installation assembly is transformed into a vertical state. At this time, the fluorescent substance on the surface of the biofilm (19) falls into the waste liquid tank (56) through the center hole of the upper annular clamp (43), thereby dumping the fluorescent substance into the waste liquid tank (56) to prepare for the second bacterial content detection.

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