Wheat coleoptile length measuring device for salt tolerance test in simulated soil environment

Abstract

The invention relates to the technical field of wheat coleoptile length measurement in a simulated soil test, and discloses a wheat coleoptile length measurement device in a simulated soil environment for a salt tolerance test, which comprises a device main body, and the device main body comprises a simulated cultivation pool in the middle and liquid supplementing boxes on the two sides of the simulated cultivation pool. Partition plates are uniformly arranged on the inner walls of the liquid supplementing box and the simulated cultivation pool, and the bottom of the liquid supplementing box on the two sides of the simulated cultivation pool is fixedly communicated with a cavity. The soil environment is simulated by arranging the multiple layers of simulation filter paper to be soaked in the wheat culture solution, and the wheat culture solution on the multiple layers of simulation filter paper is supplemented in real time through the liquid supplementing opening; a filter paper clamping frame is clamped and fixed at the periphery of the multi-layer simulation filter paper, so that the salt-tolerant growth state of the wheat seeds is recorded in a damage-free state; a rubber plate plug and a shifting piece are additionally arranged at the liquid supplementing opening, so that the device is more suitable for being taken out to observe the growth state of wheat.

Description

Technical Field

[0001] The invention relates to the technical field of wheat coleoptile length measurement in simulated soil test, in particular to a wheat coleoptile length measurement device in a simulated soil environment for salt tolerance test. Background Art

[0002] Wheat has a weak salt tolerance, which has become a limiting factor for wheat production in many saline soil areas in the world. With the increase in population and rapid industrial development in my country, arable land has dropped sharply, and unreasonable irrigation and tillage have caused the degree of soil salinization to become increasingly serious. Therefore, as the contradiction between the demand and supply of wheat becomes more and more prominent, it has become an urgent task to cultivate wheat varieties with excellent traits such as high yield and salt resistance. However, wheat salt tolerance is a relatively complex quantitative trait controlled by multiple genes, which leads to a long cycle and a large workload in wheat molecular genetic research. At the same time, since salt tolerance is a quantitative trait composed of multiple traits, it further increases the difficulty of discovering wheat salt-tolerant genes. Although many laboratories have screened out wheat varieties with strong salt tolerance by measuring the aboveground fresh weight, aboveground dry weight, root fresh weight, root dry weight, and lateral root number of experimental wheat seedlings, their salt tolerance traits are difficult to be stably inherited. There is still a big gap from cultivating salt-tolerant wheat varieties with agricultural production value and promoting them on a large scale to promote the development and utilization of saline-alkali land.

[0003] During the whole process of wheat seed germination in the soil, the coleoptile has a stronger ability to emerge from the soil than the plumule, which can protect the plumule damaged by salt from being damaged when emerging from the soil. A longer coleoptile is more conducive to the germination and growth of wheat in saline soil areas. At the same time, the heritability of the length of the wheat coleoptile is relatively strong. Therefore, by simulating the saline-alkali soil environment to measure the length of the wheat coleoptiles of different varieties, it is possible to quickly identify the salt tolerance index of wheat and also to make use of its salt tolerance trait to be stably inherited to future generations. In order to facilitate the cultivation of wheat and the observation of the growth of wheat coleoptiles, the present invention proposes a wheat coleoptile length measuring device in a simulated soil environment for salt tolerance test. Summary of the invention

[0004] In view of the deficiencies in the prior art, the present invention provides a device for measuring the length of wheat coleoptiles in a simulated soil environment for salt tolerance test, which solves the problem of inconvenience in cultivating wheat and observing the growth of wheat coleoptiles.

[0005] The object of the present invention is achieved as follows: a device for measuring the length of wheat coleoptiles in a simulated soil environment for a salt tolerance test, comprising a device body, the device body comprising a simulated cultivation pool in the middle position and liquid replenishment tanks on both sides thereof, the inner walls of the liquid replenishment tank and the simulated cultivation pool are evenly provided with partition plates, the bottoms of the liquid replenishment tanks on both sides of the simulated cultivation pool are fixedly connected with a cavity, the cavity enables the two groups of liquid replenishment tanks on both sides of the simulated cultivation pool to be connected with each other so that the height of the internal liquid level is consistent, the inner wall of the simulated cultivation pool is fixedly installed with a card slot located on both sides of the partition plate, a liquid replenishment port is opened in the groove of the card slot, and a limited flow group is arranged inside the liquid replenishment port. The flow limiting component can prevent the culture solution in the liquid replenishing tank from flowing into the simulated cultivation pool in large quantities, thereby preventing the wheat seeds from being flooded. The interior of the simulated cultivation pool is movably connected with a filter paper card frame through a card slot, and a guide tube is fixedly installed in the filter paper card frame. A gap connected to the guide tube is opened on the inner side of the filter paper card frame, and a simulated filter paper is fixedly connected with the gap through the gap. Both sides of the filter paper card frame are fixedly connected with clamping rings, and the interiors of the clamping rings on both sides are movably sleeved with lifting rods, and the bottom end of the lifting rod is fixedly installed with a lifting hook, and the lifting rods on both sides are connected by an elastic component, and the elastic component can control the clamping state of the lifting hook on the lifting rod and the clamping ring.

[0006] Furthermore, a through opening is provided at the connection between the fluid replenishment tank and the cavity, the fluid replenishment tank is connected to the simulated cultivation pool through the fluid replenishment opening, and the middle portion of the fluid replenishment opening is a cylindrical groove.

[0007] Furthermore, the flow limiting component includes a flow limiting shaft, both ends of which are installed on the central axis of the inner wall of the fluid infusion port through disc springs, rubber plugs are fixedly connected to both sides of the outer surface of the flow limiting shaft, and a paddle is fixedly connected to the side of the flow limiting shaft close to the simulated cultivation tank.

[0008] Furthermore, the filter paper card frame can slide up and down along the card slot, and the position of the liquid infusion port is aligned with the position of the guide tube.

[0009] Furthermore, the elastic component includes a telescopic sleeve, which is movably sleeved between the tops of the lifting rods on both sides. A spring is movably installed inside the telescopic sleeve, and both ends of the spring are respectively fixedly connected to the lifting rods on both sides. A pulling rope located below the telescopic sleeve is connected between the lifting rods on both sides.

[0010] Furthermore, the lifting rod is pushed by the elastic force of the spring, the pulling rope is in a straightened state, and the lifting hook at the bottom end of the lifting rod hooks the clamping ring.

[0011] Compared with the prior art, the present invention has the following beneficial effects: 1. The present invention simulates the soil environment by arranging multiple layers of simulated filter paper to soak the wheat culture solution, and realizes real-time replenishment of the wheat culture solution on the multiple layers of simulated filter paper through the liquid replenishment port; by clamping and fixing the filter paper card frame on the periphery of the multiple layers of simulated filter paper, the salt-resistant growth state of the wheat seeds is recorded in a non-damaged state; by adding a rubber plate plug and a paddle at the liquid replenishment port, the device is more suitable for taking out and observing the growth state of wheat.

[0012] 2. The present invention simulates the external environment of wheat germination in the soil environment by arranging multiple layers of simulated filter paper soaked in wheat culture solution, and utilizes the liquid replenishment box outside the simulated cultivation pool and the liquid replenishment port connected thereto to realize real-time replenishment of the wheat culture solution on the multiple layers of simulated filter paper, thereby improving the efficiency of the simulated soil environment salt tolerance test and eliminating the complicated test steps of the operator frequently replenishing the culture solution.

[0013] 3. The present invention fixes filter paper frames on the periphery of the multi-layer simulated filter paper, and uses a lifting rod to simultaneously take out multiple groups of filter paper frames to the outside of the simulated cultivation pool. The growth status of wheat germ and sheath is observed and recorded through the transparent filter paper frame frame, thereby avoiding damage to the wheat seeds when taking them out to observe growth data in the soil environment, and realizing the recording of the salt-resistant growth status of wheat seeds in a non-damaged state.

[0014] 4. The present invention adds a rubber plate plug and a paddle at the liquid replenishment port, and uses the filter paper card frame frame to press the paddle to open the rubber plate plug. When the filter paper card frame is taken out for observation, the rubber plate plug is automatically closed, thereby preventing a large amount of culture solution in the liquid replenishment tank from flowing into the simulated cultivation pool and causing flooding to the wheat seeds in the simulated cultivation pool, making the device more suitable for taking out and observing the growth status of wheat. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the provided drawings without paying creative work.

[0016] Figure 1 It is a schematic diagram of the overall structure of the present invention.

[0017] Figure 2 It is a vertical cross-sectional schematic diagram of the overall structure of the present invention.

[0018] Figure 3 It is a cross-sectional schematic diagram of the overall structure of the present invention.

[0019] Figure 4 It is a partial enlarged schematic diagram of the structure of the fluid infusion port of the present invention.

[0020] In the figure: 1. device body; 2. liquid replenishing tank; 201. cavity; 202. port; 3. simulated cultivation tank; 301. slot; 302. liquid replenishing port; 4. partition plate; 5. filter paper card frame; 501. flow guide tube; 502. simulated filter paper; 503. clamping ring; 6. lifting rod; 601. telescopic sleeve; 602. pulling rope; 7. current limiting shaft; 701. rubber plate plug; 702. pick. DETAILED DESCRIPTION

[0021] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0022] like Figure 1-4 The device for measuring the length of wheat coleoptiles in a simulated soil environment for a salt tolerance test shown in the figure comprises a device body 1, the device body 1 comprises a simulated cultivation pool 3 in the middle position and liquid replenishment tanks 2 on both sides thereof, the inner walls of the liquid replenishment tank 2 and the simulated cultivation pool 3 are evenly provided with partition plates 4, the bottoms of the liquid replenishment tanks 2 on both sides of the simulated cultivation pool 3 are fixedly connected with cavities 201, the cavities 201 enable the two groups of liquid replenishment tanks 2 on both sides of the simulated cultivation pool 3 to be connected with each other so that the heights of the internal liquid levels are consistent, the inner walls of the simulated cultivation pool 3 are fixedly provided with card slots 301 located on both sides of the partition plate 4, the grooves of the card slots 301 are provided with liquid replenishment ports 302, the liquid replenishment ports 302 connect the liquid replenishment tank 2 with the simulated cultivation pool 3, so that the culture solution in the liquid replenishment tank 2 can enter the simulated cultivation pool 3 through the liquid replenishment ports 302, a limiting flow component is provided inside the liquid replenishment port 302, the limiting flow component can prevent the culture solution in the liquid replenishment tank 2 from flowing into the simulated cultivation pool 3 in large quantities, thereby avoiding damage to the wheat seeds. The simulated culture pool 3 is flooded, the filter paper card frame 5 is movably connected to the inside of the simulated culture pool 3 through the card slot 301, and a guide tube 501 is fixedly installed in the filter paper card frame 5. A slit connected to the guide tube 501 is opened on the inside of the filter paper card frame 5, and a simulated filter paper 502 is fixedly connected to the slit, so that when the culture fluid in the liquid replenishing tank 2 enters the simulated culture pool 3 through the liquid replenishing port 302, it must first enter the guide tube 501 in the filter paper card frame 5 through the liquid replenishing port 302, and then pass through the filter paper card frame 5 The slit opened on the inner side penetrates into the simulated filter paper 502 to make it saturated with culture solution, so as to simulate the nutritional environment of wheat germ in the soil environment. The filter paper frame 5 is fixedly connected with a clamping ring 503 on both sides, and the inside of the clamping rings 503 on both sides are movably sleeved with a lifting rod 6. The lifting rod 6 is in a "7" shape, and a lifting hook is fixedly installed at the bottom end of the lifting rod 6. The lifting rods 6 on both sides are connected by an elastic component, and the elastic component can control the clamping state of the lifting hook on the lifting rod 6 and the clamping ring 503.

[0023] Furthermore, a through opening 202 is provided at the connection between the liquid replenishment tank 2 and the cavity 201 , and the liquid replenishment tank 2 and the simulated cultivation pool 3 are connected through a liquid replenishment port 302 , and the middle of the liquid replenishment port 302 is a cylindrical groove.

[0024] Furthermore, the current limiting component includes a current limiting shaft 7, both ends of which are installed on the central axis of the inner wall of the fluid infusion port 302 through disc springs, rubber plate plugs 701 are fixedly connected to both sides of the outer surface of the current limiting shaft 7, and a paddle 702 is fixedly connected to the side of the current limiting shaft 7 close to the simulated cultivation tank 3.

[0025] When the paddle 702 is pressed downward, the flow limiting shaft 7 will be pushed to make the rubber plug 701 rotate and open. When the paddle 702 is no longer under force, the disc springs at both ends of the flow limiting shaft 7 drive the flow limiting shaft 7 to reverse back to the rubber plug 701 to close the liquid replenishing port 302, thereby ensuring that the liquid replenishing port 302 opened in the card slot 301 and connected to the liquid replenishing tank 2 is closed under normal conditions, and only when the filter paper card frame 5 is pushed inward, the conduction is opened under the pressure of the frame of the filter paper card frame 5, thereby preventing a large amount of culture fluid in the liquid replenishing tank 2 from flowing into the simulated cultivation pool 3 and causing flooding to the wheat seeds in the simulated cultivation pool 3.

[0026] Furthermore, the filter paper card frame 5 can slide up and down along the card slot 301 , and the position of the liquid infusion port 302 is aligned with the position of the flow guide tube 501 .

[0027] Furthermore, the elastic component includes a telescopic sleeve 601, which is movably sleeved between the tops of the lifting rods 6 on both sides. A spring is movably installed inside the telescopic sleeve 601, and both ends of the spring are respectively fixedly connected to the lifting rods 6 on both sides. A pulling rope 602 located below the telescopic sleeve 601 is connected between the lifting rods 6 on both sides.

[0028] Furthermore, the lifting rod 6 is pushed by the elastic force of the spring, the pulling rope 602 is in a straightened state, and the lifting hook at the bottom end of the lifting rod 6 hooks the clamping ring 503.

[0029] When the pulling rope 602 is in the normal straight state, the lifting rod 6 is pushed outward by the spring, and the lifting hook at the bottom thereof hooks the clamping ring 503 here. At this time, the telescopic sleeve 601 is pulled upward to pull the filter paper frame 5 as a whole upward to the outside of the simulated cultivation pool 3, so that the operator can observe the growth state of the wheat germ from the side, and observe the number of layers of the simulated filter paper 502 in the filter paper frame 5 that are punctured by the growth of the wheat coleoptile. After the observation is completed, the telescopic sleeve 601 is lowered to put the filter paper frame 5 back into the simulated cultivation pool 3, and the pulling rope 602 is pulled to pull the lifting rod 6 toward the middle. At this time, the lifting hook at the bottom of the lifting rod 6 is The hook is detached from the clamping ring 503, and the pulling rope 602 can be easily pulled out by pulling it upwards. Limiting devices are provided at both ends of the telescopic sleeve 601 to prevent the lifting rod 6 from being detached from the telescopic sleeve 601 due to the spring force after being taken out. At this time, the lifting rod 6 can be extended into the clamping rings 503 on both sides of the other group of filter paper card frames 5 to perform in vivo observation operations. Under the premise of not damaging the wheat germ, the growth state of wheat in the simulated soil environment can be conveniently measured, and the growth ability of the coleoptile can be quickly recorded by recording the number of damaged layers of the simulated filter paper 502, thereby realizing efficient and rapid identification of wheat salt tolerance indicators to obtain high heritability flooding tolerance traits.

[0030] The working principle of the method of the present invention is as follows: During use, wheat culture solution with gradient salinity is injected into each group of liquid replenishing tanks 2 separated by the partition plate 4. Due to the connecting effect of the bottom cavity 201, the liquid level heights in the liquid replenishing tanks 2 on both sides of each group are consistent. At this time, the liquid replenishing port 302 connected to the simulated cultivation pool 3 is closed by the rubber plate plug 701 under the action of the coil spring because the paddle 702 is not pressed. Then, a filter paper card frame 5 with simulated filter paper 502 is pushed into each group of simulated cultivation pool 3 at a time. During the pushing process, the frame of the filter paper card frame 5 presses the paddle 702 at the groove of the card slot 301 from top to bottom in turn, so that the liquid replenishing port 302 at the path is opened in turn to let water into the guide tube 501 and then closed until the filter paper card frame 5 falls to the lowest end and continues. The paddle 702 in the lowest liquid replenishment port 302 is pressed to make this place connected, and the liquid first enters the guide tube 501 in the filter paper card frame 5 through the liquid replenishment port 302, and then penetrates into the simulated filter paper 502 through the slit opened on the inner side of the filter paper card frame 5 to make the simulated filter paper 502 soaked in the culture solution. At this time, under the action of water surface tension, the culture solution no longer flows out from the slit, but continues to be replenished as the culture solution on the simulated filter paper 502 evaporates or is consumed. After the wheat culture solution is infiltrated on the lowest layer of simulated filter paper 502, the wheat plants to be tested are evenly placed on the lowest layer of simulated filter paper 502, and then multiple groups of filter paper card frames 5 on the upper side are placed in each group of simulated cultivation pools 3 to simulate the dark oppressive environment under the soil environment. Each group of filter paper card frames The liquid inlet 302 at 5 is opened under pressure to infuse the layer of simulated filter paper 502 with liquid, so as to simulate the nutritional environment in the soil. During the cultivation process, the lifting rod 6 is used to regularly take out each group of filter paper frames 5 for observation. During the taking-out process, the vertical section of the lifting rod 6 is extended into the clamping rings 503 at both ends of the group of filter paper frames 5 until the bottom clamping ring 503. When the pulling rope 602 is in the normal straight state, the lifting rod 6 is pushed outward by the spring, and the lifting hook at the bottom end thereof hooks the clamping ring 503 here. At this time, the telescopic sleeve 601 is pulled upward to pull the group of filter paper frames 5 as a whole upward to the outside of the simulated cultivation pool 3, so that the operator can observe the growth state of the wheat germ from the side, and observe the simulated filter paper 502 in the group of filter paper frames 5. The number of layers of wheat coleoptile growth puncture, the paddle 702 is automatically closed when not under pressure during the process of taking out the filter paper card frame 5, avoiding the problem that the liquid infusion port 302 is connected to the liquid infusion tank 2 and the simulated cultivation pool 3 during the observation, resulting in the simulated cultivation pool 3 being filled with culture solution. After the observation is completed, the telescopic sleeve 601 is lowered to put the group of filter paper card frames 5 back into the simulated cultivation pool 3, and the pulling rope 602 is pulled to pull the pulling rod 6 toward the middle. At this time, the lifting hook at the bottom end of the lifting rod 6 is separated from the clamping ring 503, and the pulling rope 602 can be pulled upward to easily take it out. Similarly, the wheat growth data in multiple groups of simulated cultivation pools 3 are observed and recorded in turn, avoiding the problem of irreversible damage to the wheat germ caused by taking out the observation records in the soil environment.

[0031] The above embodiments are only used to help understand the method and core idea of ​​the present invention. It should be noted that, for those skilled in the art, several improvements and modifications can be made to the present invention without departing from the principles of the present invention, and these improvements and modifications also fall within the scope of protection of the claims of the present invention.

Claims

1. A device for measuring the length of wheat coleoptiles in a simulated soil environment for salt tolerance test, comprising a device body (1), characterized in that: The device body (1) comprises a simulated cultivation pool (3) in the middle and liquid replenishment tanks (2) on both sides thereof; the inner walls of the liquid replenishment tank (2) and the simulated cultivation pool (3) are evenly provided with partition plates (4); the liquid replenishment tanks (2) on both sides of the simulated cultivation pool (3) are fixedly connected with cavities (201) at the bottom; the cavities (201) enable the two groups of liquid replenishment tanks 2 on both sides of the simulated cultivation pool 3 to be connected with each other so that the height of the internal liquid levels is consistent; the inner wall of the simulated cultivation pool (3) is fixedly provided with card slots (301) located on both sides of the partition plate (4); a liquid replenishment port (302) is provided in the groove of the card slot (301); a limiting flow component is provided inside the liquid replenishment port (302); the limiting flow component can prevent a large amount of culture fluid in the liquid replenishment tank (2) from flowing into the simulated cultivation pool (3) In a cultivation pool (3), the wheat seeds are prevented from being flooded. A filter paper card frame (5) is movably connected to the interior of the simulated cultivation pool (3) through a card slot (301). A flow guide tube (501) is fixedly installed in the filter paper card frame (5). A slit communicating with the flow guide tube (501) is provided on the inside of the filter paper card frame (5), and a simulated filter paper (502) is fixedly connected to the slit. A clamping ring (503) is fixedly connected to both sides of the filter paper card frame (5). Lifting rods (6) are movably sleeved inside the clamping rings (503) on both sides. A lifting hook is fixedly installed at the bottom end of the lifting rod (6). The lifting rods (6) on both sides are connected by an elastic component. The elastic component can control the clamping state of the lifting hook on the lifting rod (6) and the clamping ring (503).

2. The device for measuring wheat coleoptile length in a simulated soil environment for salt tolerance test according to claim 1, characterized in that: A through opening (202) is provided at the connection between the liquid replenishment box (2) and the cavity (201); the liquid replenishment box (2) and the simulated cultivation pool (3) are connected via a liquid replenishment opening (302); the middle portion of the liquid replenishment opening (302) is a cylindrical groove.

3. The device for measuring wheat coleoptile length in a simulated soil environment for salt tolerance test according to claim 1, characterized in that: The flow limiting assembly comprises a flow limiting shaft (7), the two ends of which are mounted on the central axis of the inner wall of the liquid replenishing port (302) via a coil spring, the two sides of the outer surface of the flow limiting shaft (7) are fixedly connected to rubber plate plugs (701), and the side of the flow limiting shaft (7) close to the simulated cultivation pool (3) is fixedly connected to a paddle (702).

4. The device for measuring wheat coleoptile length in a simulated soil environment for salt tolerance test according to claim 1, characterized in that: The filter paper card frame (5) can slide up and down along the card slot (301), and the position of the liquid replenishing port (302) is aligned with the position of the flow guide tube (501).

5. The device for measuring wheat coleoptile length in a simulated soil environment for salt tolerance test according to claim 1, characterized in that: The elastic component comprises a telescopic sleeve (601), the telescopic sleeve (601) being movably sleeved between the tops of the lifting rods (6) on both sides, a spring being movably installed inside the telescopic sleeve (601), and the two ends of the spring being respectively fixedly connected to the lifting rods (6) on both sides, and a pulling rope (602) located below the telescopic sleeve (601) is connected between the lifting rods (6) on both sides.

6. The device for measuring wheat coleoptile length in a simulated soil environment for salt tolerance test according to claim 5, characterized in that: The lifting rod (6) is pushed by the elastic force of the spring, the pulling rope (602) is in a straightened state, and the lifting hook at the bottom end of the lifting rod (6) hooks the clamping ring (503).

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