Novel amine molecular group detection method and device

Abstract

The invention discloses a novel amine molecular group detection method and device, and relates to the technical field of chemical analys.The method comprises the following steps that an irradiation light source is selected based on a light source judgment rule, and the optimal pulse frequency of the irradiation light source is determined; controlling the irradiation light source to be turned on, and filtering light of the irradiation light source by using a filtering unit; irradiating the detected sample by using the filtered light, controlling the irradiation light source to be turned off, and collecting a fluorescence signal of the detected sample; and based on the collected fluorescence signal, recording a fluorescence spectrum by using a fluorescence spectrometer, and judging the content of the amine molecular groups in the detection sample according to the fluorescence spectrum. According to the invention, amine molecular group fluorescence detection is carried out based on the specific pulse frequency light source, the sensitivity is high, the stability is good, the operation is simple and convenient, and efficient and accurate detection of amine molecular groups is realized by optimizing light source selection, pulse frequency setting, fluorescence signal acquisition and optimization, fluorescence spectrum determination and data analysis.

Description

Technical Field

[0001] The present invention relates to the technical field of chemical analysis, and in particular to a novel method and device for detecting amine molecular groups. Background Art

[0002] Amine molecular clusters play a key role in many fields such as biology, medicine, and chemical industry. In biological systems, biogenic amines such as histamine and dopamine act as neurotransmitters or signaling molecules and participate in regulating a variety of physiological functions, such as mood changes, sleep cycles, pain perception, etc. The balance of these biogenic amines is essential for maintaining the health of organisms; in the field of medicine, amine molecular clusters are important components of many drugs, and they play a key role in the treatment of hypertension, heart disease, depression and other diseases; and in the chemical industry, amine molecular clusters are used as raw materials for the synthesis of various chemicals, such as pesticides, dyes, surfactants, etc. Therefore, accurate and rapid detection of amine molecular clusters is extremely important for scientific research, drug development and industrial production.

[0003] Although the detection of amine molecular groups is very important, existing detection technologies, such as gas chromatography-mass spectrometry (GC-MS) and high-performance liquid chromatography (HPLC), have some obvious limitations. The operation process of these technologies is relatively cumbersome and requires professional technicians to operate. They are easily interfered by other components in the sample, especially in complex sample matrices, such as food, biological samples or environmental samples. These interference factors may significantly affect the test results, leading to false positive or false negative results, increasing analytical errors, and thus reducing the accuracy and sensitivity of the test; at the same time, these detection technologies require a high level of technical skills of the operators, usually requiring complex sample pretreatment steps, expensive equipment and long analysis time, which not only increases the complexity of the operation, but also limits their application in rapid detection and on-site analysis.

[0004] Traditional amine molecular cluster detection technology has the problems of low sensitivity, complex operation, and many interference factors, which cannot meet the needs of modern scientific research and industrial production. Therefore, a new amine molecular cluster detection method is urgently needed. The laser light source is controlled by a specific pulse frequency. When the light source is on, the light is reflected by the mirror and focused by the focusing plate, and the focused light is reflected to the interference filter. The light passing through the interference filter is then focused and introduced into the optical fiber. The light passing through the optical fiber enters the smooth mirror channel to irradiate the sample containing amine molecular clusters, effectively stimulating the fluorescence effect of the amine molecular clusters, ensuring the stability of the fluorescence signal collection, and realizing efficient and accurate detection of amine molecular clusters.

[0005] Currently, no effective solution has been proposed for the problems in the related technologies. Summary of the invention

[0006] In response to the problems in the related art, the present invention proposes a novel method and device for detecting amine molecular clusters, which have the advantages of effectively stimulating the fluorescence effect of amine molecular clusters, ensuring the stability of fluorescence signal collection, and achieving efficient and accurate detection of amine molecular clusters, thereby solving the problems in the prior art of low sensitivity, complex operation, and many interference factors, and being unable to meet the needs of modern scientific research and industrial production.

[0007] To this end, the specific technical solution adopted by the present invention is as follows: According to one aspect of the present invention, a novel method for detecting amine molecular clusters is provided, and the novel method for detecting amine molecular clusters comprises the following steps: S1. Selecting an irradiation light source based on a light source determination rule and determining an optimal pulse frequency of the irradiation light source; S2, controlling the illumination light source to turn on, and using the filtering unit to filter the light of the illumination light source; S3, using the filtered light to illuminate the test sample, controlling the irradiation light source to turn off and collecting the fluorescence signal of the test sample; S4. Based on the collected fluorescence signal, the fluorescence spectrum is recorded using a fluorescence spectrometer, and the amine molecular cluster content of the test sample is determined according to the fluorescence spectrum.

[0008] Further, selecting an illumination light source based on the light source determination rule and determining an optimal pulse frequency of the illumination light source includes the following steps: S11, selecting an illumination light source from candidate light sources based on a light source determination rule; S12, setting the excitation wavelength range according to the absorption peak, and determining the excitation wavelength based on the wavelength constraint rule; S13. Determine the optimal pulse frequency of the irradiation light source based on the excitation wavelength and target characteristics.

[0009] Further, based on the light source determination rule, selecting the illumination light source from the candidate light sources includes the following steps: S111, based on each candidate light source, measuring the excitation wavelength and emission wavelength of the fluorescent substance, and determining the intensity of the fluorescence effect according to the fluorescence spectrum of the fluorescent substance; S112. According to the measurement results, if the excitation wavelength of the fluorescent substance is always within the excitation wavelength range of the amine reactant in the fluorescent reaction chamber, the score of the corresponding candidate light source is increased by 1; S113. According to the analysis results, if the fluorescence intensity of the amine fluorescent substance remains unchanged for more than half of the measurement time, the score of the corresponding candidate light source is increased by 1; S114. Count the scores of all candidate light sources, and select the candidate light source with the highest score as the illumination light source.

[0010] Further, the excitation wavelength range set according to the absorption peak is 490-570nm; The wavelength constraint rule is: the excitation wavelength is shorter than the filtering wavelength of the long-pass filter.

[0011] Further, based on the excitation wavelength and the fluorescence signal characteristics, determining the optimal pulse frequency of the illumination light source includes the following steps: S131, performing an initial experiment based on the irradiation light source, and recording the initial response of the fluorescent substance to different pulse frequencies; S132, gradually increasing the pulse frequency of the irradiation light source, and recording the intensity of the fluorescence signal; S133. When the fluorescence signal intensity is stable and the acquisition signal rate is stable, the pulse frequency at this time is selected as the optimal pulse frequency.

[0012] Furthermore, using the filtered light to illuminate the test sample, controlling the illumination light source to turn off and collecting the fluorescence signal of the test sample includes the following steps: S31, irradiating the test sample with the light filtered by the filter unit to cause the amine molecular clusters to produce a fluorescent effect; S32, optimizing the reaction conditions and controlling the duty cycle of the laser light source to ensure the stability of fluorescence signal collection; S33, turn off the illumination light source, and collect the fluorescence signal of the amine molecular cluster in the smooth mirror channel at the long-pass filter.

[0013] Further, optimizing the reaction conditions and controlling the duty cycle of the laser light source to ensure the stability of fluorescence signal collection includes the following steps: S321, adjusting the pH value and concentration of the reactants by adding a buffer; S322, using a temperature controller to control the temperature in the fluorescence reaction chamber; S323, adjusting the duty cycle of the excitation light source to match the stable collection of the fluorescence signal.

[0014] According to another aspect of the present invention, a novel amine molecular group detection device is also provided, and the novel amine molecular group detection device comprises: an irradiation light source, a reflector, a filtering unit, an optical fiber, a smooth mirror channel, a photomultiplier tube, a control unit and a data analysis unit; Wherein, the irradiation light source is used to emit laser light of a specific frequency; The reflector, filter unit and optical fiber are used for filtering, focusing and transmitting the light emitted by the irradiation light source; The filtering unit includes a condenser, a monochromatic filter and a long-pass filter, which are used to filter the light emitted by the irradiation light source; The smooth mirror channel is used to assist the laser irradiation detection sample and the reflection of the fluorescence signal; The photomultiplier tube is used to measure the fluorescence signal of the test sample and record the change of the fluorescence signal; The control unit is used to control the pulse frequency and duty cycle of the irradiation light source, and to receive and process the fluorescence signal data measured by the photomultiplier tube; The data analysis unit is used to determine the content of amine molecular clusters in the test sample according to the change of the fluorescence signal.

[0015] Furthermore, judging the content of amine molecular groups in the test sample according to the change of the fluorescence signal includes: Prepare a series of amine standard solutions of known concentrations; Based on the amine standard solution, a standard curve diagram between the fluorescence signal and the standard concentration of the amine is drawn, wherein the X-axis of the standard curve diagram is the concentration of the amine in the standard solution, and the Y-axis is the corresponding fluorescence signal value; Based on the test sample, a test curve diagram between the fluorescence signal and the concentration of the amine molecular group is drawn, wherein the X-axis of the test curve diagram is the concentration of the amine molecular group in the test sample, and the Y-axis is the corresponding fluorescence signal value; The measured test curve graph is compared with the standard curve graph, and the content of amine molecular groups in the test sample is determined.

[0016] Further, the measured detection curve graph is compared with the standard curve graph, and the content of the amine molecular group in the detection sample is determined to include: Compare the test curve with the standard curve. If the fluorescence signal value of the test sample is close to a point in the standard curve, it is determined that amine molecular clusters exist in the test sample, and the content of amine molecular clusters is estimated based on the corresponding point in the standard curve. If the fluorescence signal value of the test sample is much lower than the lowest point in the standard curve, it is judged that there is no or only a very small amount of amine molecular clusters in the test sample.

[0017] The beneficial effects of the present invention are: (1) High sensitivity and stability: The present invention controls the on and off of the laser light source through a specific pulse frequency set artificially, which effectively stimulates the fluorescence effect of the amine molecular clusters. When the light source is off, the fluorescence signal in the smooth mirror channel is collected, avoiding the interference of the light source itself on the signal collection, thereby improving the sensitivity of the detection; at the same time, by optimizing the reaction conditions and controlling the pulse frequency, the stability of the fluorescence signal collection is ensured, making the detection results more accurate and reliable.

[0018] (2) Innovation of the new detection technology: The present invention proposes a new amine molecular cluster detection technology, which uses a light source controlled by a specific pulse frequency to perform on-and-off irradiation and collects fluorescent signals when the light source is off. This innovative detection method not only simplifies the traditional detection process, but also improves the detection efficiency, providing a new technical means for the detection of amine molecular clusters.

[0019] (3) Wide applicability and practicality: The detection technology of the present invention can be widely used in the detection of various samples containing amine molecular clusters, not just limited to specific types of samples. At the same time, the fluorescence spectrum of the sample after the reaction is measured by a fluorescence spectrometer, which can intuitively record the changes in the fluorescence signal, providing a strong basis for judging the presence and content of amine molecular clusters in the sample. It has strong practicality and broad application prospects.

[0020] (4) Non-destructive detection process: The present invention does not destroy the amine molecular clusters in the sample during the detection process, which means that the integrity of the sample can be maintained after the detection, facilitating subsequent analysis and processing.

[0021] (5) Convenience and energy saving: The detection process of the present invention is relatively simple. It only needs to measure the fluorescence spectrum of the sample after the reaction through a fluorescence spectrometer to intuitively record the changes in the fluorescence signal, making the operation easier. In addition, by precisely controlling the on and off of the light source and the pulse frequency, the present invention achieves effective utilization of energy, reduces unnecessary energy consumption, and complies with the concept of environmental protection and energy saving. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0023] Figure 1 is a schematic flow chart of a novel amine molecular group detection method according to an embodiment of the present invention; Figure 2 It is a schematic structural diagram of a novel amine molecular cluster detection device according to an embodiment of the present invention.

[0024] In the figure: 1. Light source; 2. Reflector; 3. Filter unit; 301. Focusing plate; 302. Monochromatic filter; 303. Long-pass filter; 4. Optical fiber; 5. Smooth mirror channel; 6. Photomultiplier tube; 7. Control unit; 8. Data analysis unit; 9. Fluorescence reaction chamber; 10. Fluorescence. DETAILED DESCRIPTION

[0025] To further illustrate each embodiment, the present invention provides drawings, which are part of the disclosure of the present invention and are mainly used to illustrate the embodiments and can be used in conjunction with the relevant descriptions in the specification to explain the operating principles of the embodiments. With reference to these contents, ordinary technicians in the field should be able to understand other possible implementations and advantages of the present invention. The components in the figures are not drawn to scale, and similar component symbols are generally used to represent similar components.

[0026] According to an embodiment of the present invention, a novel method for detecting amine molecular clusters is provided.

[0027] The present invention is further described with reference to the accompanying drawings and specific embodiments. Figure 1 As shown, according to one embodiment of the present invention, a novel method for detecting amine molecular clusters is provided, and the novel method for detecting amine molecular clusters comprises the following steps: According to one aspect of the present invention, a novel method for detecting amine molecular clusters is provided, and the novel method for detecting amine molecular clusters comprises the following steps: S1. Selecting an irradiation light source based on a light source determination rule and determining an optimal pulse frequency of the irradiation light source; S2, controlling the illumination light source to turn on, and using the filtering unit 3 to filter the light of the illumination light source; S3, using the filtered light to illuminate the test sample, controlling the irradiation light source to turn off and collecting the fluorescence signal of the test sample; S4. Based on the collected fluorescence signal, the fluorescence spectrum is recorded using a fluorescence spectrometer, and the amine molecular cluster content of the test sample is determined according to the fluorescence spectrum.

[0028] Specifically, first, a laser light source of a specific frequency is selected, and the light source is controlled by a specific pulse frequency set manually. The laser passes through a reflector, a condenser, a monochromatic filter, and the light filtered by a long-pass filter passes through an optical fiber, and the amine molecular group will produce a fluorescence effect under the irradiation, so that when the light source is off, the amine molecular group fluorescence signal generated by the fluorescence reflected in the smooth mirror channel is collected at the long-pass filter. During the collection process, the stability of the fluorescence signal collection is ensured by optimizing the reaction conditions and controlling the pulse frequency.

[0029] Specifically, secondly, a new type of amine molecular cluster detection technology is established by using the light source controlled by the above-selected specific pulse frequency, including: irradiating the sample to be tested with a laser of a selected specific sampling frequency in a light-on-off form, and then collecting the fluorescence signal in the mirror channel when the light source is off, using a photomultiplier tube to measure the fluorescence signal of the sample after the reaction, and recording the change of the fluorescence signal; finally, judging the presence and content of amine molecular clusters in the sample based on the change of the fluorescence signal.

[0030] Specifically, the steps for detecting amine molecular clusters are as follows: a. Select a specific irradiation light source, and the light source is controlled to turn on and off by a specific pulse frequency set manually; b. The pulse frequency is selected based on the excitation wavelength, and the excitation wavelength is selected near the absorption peak and less than the filtering wavelength of the long-pass filter, in the range of (490-570nm); c. Under the irradiation of light filtered by the light source through a condenser, a monochromatic filter, and a long-pass filter, the amine molecular clusters produce a fluorescence effect; d. When the light source is off, the fluorescence signal of the amine molecular clusters in the smooth mirror channel is collected at the long-pass filter; e. During the collection process, the stability of the fluorescence signal collection is ensured by optimizing the reaction conditions and controlling the laser duty cycle.

[0031] In one embodiment, selecting an illumination light source based on a light source determination rule and determining an optimal pulse frequency of the illumination light source comprises the following steps: S11, selecting an illumination light source from candidate light sources based on a light source determination rule; S12, setting the excitation wavelength range according to the absorption peak, and determining the excitation wavelength based on the wavelength constraint rule; S13. Determine the optimal pulse frequency of the irradiation light source based on the excitation wavelength and target characteristics.

[0032] Specifically, the first step of the present invention is to select a laser of a specific frequency. The light source has a specific pulse frequency set artificially to control its on and off state. The selection of the light source needs to be based on its ability to produce a stable and strong fluorescence effect, so that when irradiating the amine molecular group, it can effectively excite fluorescence and reflect it to the long-pass filter to collect the fluorescence signal. Since the excitation wavelength ranges of different amine substances are different, and the setting of the pulse frequency is determined according to the excitation wavelength of fluorescence, a higher pulse frequency means that more photons are used to excite the fluorescent substance. The appropriate excitation wavelength can ensure that the fluorescent substance is effectively excited and produces a strong fluorescence signal. In this embodiment, the excitation wavelength is selected near the absorption peak, and the excitation wavelength must be set to be less than the filtering wavelength of the long-pass filter. After the excitation wavelength is selected, an initial experiment is performed to understand the response of the fluorescent substance to different pulse frequencies. By gradually increasing the pulse frequency, the intensity and stability of the fluorescence signal are observed until the fluorescence intensity is stable and the rate of collecting the signal tends to be stable. At this moment, the optimal pulse frequency range is found to ensure that the changes in the fluorescence signal can be accurately captured during the on and off process of the light source.

[0033] In one embodiment, based on the light source determination rule, selecting an illumination light source from the candidate light sources comprises the following steps: S111, based on each candidate light source, measuring the excitation wavelength and emission wavelength of the fluorescent substance, and determining the intensity of the fluorescence effect according to the fluorescence spectrum of the fluorescent substance; S112. According to the measurement results, if the excitation wavelength of the fluorescent substance is always within the excitation wavelength range of the amine reactant in the fluorescent reaction chamber, the score of the corresponding candidate light source is increased by 1; S113. According to the analysis results, if the fluorescence intensity of the amine fluorescent substance remains unchanged for more than half of the measurement time, the score of the corresponding candidate light source is increased by 1; S114. Count the scores of all candidate light sources, and select the candidate light source with the highest score as the illumination light source.

[0034] Specifically, the selection of the illumination light source needs to be based on its ability to produce a stable and strong fluorescence effect, and the steps for determining the ability are as follows: a. Measure the excitation wavelength and emission wavelength of the fluorescent substance under the selected light source, analyze the characteristics of its fluorescence spectrum, such as spectral band width, peak position, etc., and determine the stability and intensity of the fluorescence effect; b. The excitation wavelength is stable if it is always within the excitation wavelength range of the amine reactants in the reaction chamber; c. If the amine fluorescent substance maintains a stable fluorescence intensity for more than half of the measurement time, then its fluorescence effect can be considered to be strong.

[0035] In one embodiment, the excitation wavelength range set according to the absorption peak is 490-570nm; The wavelength constraint rule is: the excitation wavelength is shorter than the filtering wavelength of the long-pass filter.

[0036] Specifically, the excitation wavelength is selected to be near the absorption peak of the fluorescent substance, and the excitation wavelength is shorter than the filtering wavelength of the long pass filter.

[0037] In one embodiment, determining the optimal pulse frequency of the illumination light source based on the excitation wavelength and the fluorescence signal characteristics comprises the following steps: S131, performing an initial experiment based on the irradiation light source, and recording the initial response of the fluorescent substance to different pulse frequencies; S132, gradually increasing the pulse frequency of the irradiation light source, and recording the intensity of the fluorescence signal; S133. When the fluorescence signal intensity is stable and the acquisition signal rate is stable, the pulse frequency at this time is selected as the optimal pulse frequency.

[0038] In one embodiment, using filtered light to illuminate the detection sample, controlling the illumination light source to turn off, and collecting the fluorescence signal of the detection sample includes the following steps: S31, irradiating the test sample with the light filtered by the filter unit 3, so that the amine molecular clusters produce a fluorescent effect; S32, optimizing the reaction conditions and controlling the duty cycle of the laser light source to ensure the stability of fluorescence signal collection; S33 , turning off the illumination light source, and collecting the fluorescence signal of the amine molecular group in the smooth mirror channel 5 at the long-pass filter 303 .

[0039] Specifically, in this embodiment, the fluorescence signal is collected by performing the collection in the smooth mirror channel 5 when the light source is off, so as to ensure that the collected signal truly reflects the existence and content of the amine molecular clusters.

[0040] Specifically, the collected fluorescence signal will be measured by a fluorescence spectrometer to record the changes in the fluorescence spectrum. Based on the characteristics of the fluorescence spectrum, the presence and content of amine molecular clusters in the sample can be determined. During the data analysis process, the fluorescence signal is quantitatively and qualitatively analyzed by combining statistical and chemical principles to improve the accuracy and reliability of the test results.

[0041] In one embodiment, optimizing the reaction conditions and controlling the duty cycle of the laser light source to ensure the stability of fluorescence signal collection includes the following steps: S321, adjusting the pH value and concentration of the reactants by adding a buffer; S322, using a temperature controller to control the temperature in the fluorescence reaction chamber; S323, adjusting the duty cycle of the excitation light source to match the stable collection of the fluorescence signal.

[0042] Specifically, during the fluorescence signal acquisition process, the duty cycle of the laser light source is adjusted to match the acquisition stability of the fluorescence signal to optimize the acquisition effect of the fluorescence signal.

[0043] Specifically, under the irradiation of the light source, the amine molecular clusters will produce a fluorescence effect. In this embodiment, the fluorescence signal of the amine molecular clusters in the smooth mirror channel is collected when the light source is off. During the collection process, in order to ensure the stability of the fluorescence signal collection, the reaction conditions can be optimized by adding a buffer to control the pH value and concentration of the reactants, and a temperature controller can be used to control the temperature in the fluorescence reaction chamber. At the same time, the duty cycle of the excitation light source is changed to match the stability of the fluorescence signal collection, because a lower duty cycle means a lower repetition frequency of the laser pulse, which may lead to insufficient collection of the fluorescence signal, and a higher duty cycle may lead to excessive excitation or saturation of the fluorescence signal. Therefore, as the fluorescence signal is collected, it is necessary to adjust the duty cycle to match the stability of the fluorescence signal collection.

[0044] like Figure 2 As shown, according to another embodiment of the present invention, a novel amine molecular group detection device is also provided, and the novel amine molecular group detection device comprises: an irradiation light source 1, a reflector 2, a filter unit 3, an optical fiber 4, a smooth mirror channel 5, a photomultiplier tube 6, a control unit 7 and a data analysis unit 8; Wherein, the irradiation light source 1 is used to emit laser light of a specific frequency; The reflector 2, the filter unit 3 and the optical fiber 4 are used for filtering, focusing and transmitting the light emitted by the irradiation light source; The filter unit 3 includes a condenser 301, a monochromatic filter 302 and a long-pass filter 303, which are used to filter the light emitted by the illumination light source; The smooth mirror channel 5 is used to assist the laser irradiation detection sample and the reflection of the fluorescence signal; Specifically, the inner wall of the fluorescence reaction chamber 9 is a smooth mirror channel 5; The photomultiplier tube 6 is used to measure the fluorescence signal of the detection sample and record the change of the fluorescence signal; The control unit 7 is used to control the pulse frequency and duty cycle of the irradiation light source, and to receive and process the fluorescence signal data measured by the photomultiplier tube; The data analysis unit 8 is used to determine the content of amine molecular groups in the test sample according to the change of the fluorescence signal.

[0045] In one embodiment, judging the content of amine molecular groups in the test sample according to the change of the fluorescent signal includes: Prepare a series of amine standard solutions of known concentrations; Based on the amine standard solution, a standard curve diagram between the fluorescence signal and the standard concentration of the amine is drawn, wherein the X-axis of the standard curve diagram is the concentration of the amine in the standard solution, and the Y-axis is the corresponding fluorescence signal value; Based on the test sample, a test curve diagram between the fluorescence signal and the concentration of the amine molecular group is drawn, wherein the X-axis of the test curve diagram is the concentration of the amine molecular group in the test sample, and the Y-axis is the corresponding fluorescence signal value; The measured test curve graph is compared with the standard curve graph, and the content of amine molecular groups in the test sample is determined.

[0046] Specifically, in this embodiment, the data analysis system 8 is used to determine the presence and content of amine molecular clusters in the sample according to the change of the fluorescence signal. The specific steps are: a. draw a standard curve between the fluorescence signal and the concentration of the amine molecular clusters to compare the measured sample fluorescence signal with the standard curve; if the fluorescence signal of the sample matches or is close to a point on the standard curve, it can be determined that there are amine molecular clusters in the sample, and its content can be estimated by the corresponding point on the standard curve. If the fluorescence signal of the sample is much lower than the lowest point on the standard curve, it may indicate that there are no or only a very small amount of amine molecular clusters in the sample; b. draw a standard curve, prepare a series of amine standard solutions of known concentrations, and use the experimental steps of the present invention to draw a standard curve between the fluorescence signal and the amine concentration. In the above embodiment, this is accomplished by drawing a chart in which the X-axis is the concentration of the amine and the Y-axis is the corresponding fluorescence signal value.

[0047] In one embodiment, comparing the detected detection curve with the standard curve and determining the content of the amine molecular group in the detection sample includes: Compare the test curve with the standard curve. If the fluorescence signal value of the test sample is close to a point in the standard curve, it is determined that amine molecular clusters exist in the test sample, and the content of amine molecular clusters is estimated based on the corresponding point in the standard curve. If the fluorescence signal value of the test sample is much lower than the lowest point in the standard curve, it is judged that there is no or only a very small amount of amine molecular clusters in the test sample.

[0048] In summary, with the aid of the above technical solution of the present invention, the on and off of the laser light source is controlled by artificially setting a specific pulse frequency, so that the fluorescence effect of the amine molecular clusters is effectively stimulated. When the light source is off, the fluorescence signal in the smooth mirror channel is collected, avoiding the interference of the light source itself on the signal collection, thereby improving the sensitivity of the detection. At the same time, by optimizing the reaction conditions and controlling the pulse frequency, the stability of the fluorescence signal collection is ensured, making the detection result more accurate and reliable. The present invention proposes a novel amine molecular cluster detection technology, which uses a light source controlled by a specific pulse frequency to perform on-off irradiation, and collects fluorescence signals when the light source is off. This innovative detection method not only simplifies the traditional detection process, but also improves the detection efficiency, and provides a new technical means for the detection of amine molecular clusters. The detection technology of the present invention can be widely used in various In the detection of samples containing amine molecular groups, it is not limited to a specific type of samples. The fluorescence spectrum of the sample after the reaction is measured by a fluorescence spectrometer, and the change of the fluorescence signal can be intuitively recorded, which provides a strong basis for judging the presence and content of the amine molecular groups in the sample, and has strong practicality and broad application prospects. The present invention will not destroy the amine molecular groups in the sample during the detection process, which means that the integrity of the sample can be maintained after the detection, which is convenient for subsequent analysis and processing. The detection process of the present invention is relatively simple, and the change of the fluorescence signal can be intuitively recorded by only performing fluorescence spectrum measurement on the sample after the reaction through a fluorescence spectrometer, making the operation more simple and easy. In addition, by accurately controlling the on and off of the light source and the pulse frequency, the present invention realizes the effective utilization of energy, reduces unnecessary energy consumption, and conforms to the concept of environmental protection and energy saving.

[0049] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A novel method for detecting amine molecular groups, characterized in that: The novel amine molecular group detection method comprises the following steps: S1. Selecting an irradiation light source based on a light source determination rule and determining an optimal pulse frequency of the irradiation light source; S2, controlling the illumination light source to turn on, and using the filtering unit to filter the light of the illumination light source; S3, using the filtered light to illuminate the test sample, controlling the irradiation light source to turn off and collecting the fluorescence signal of the test sample; S4. Based on the collected fluorescence signal, the fluorescence spectrum is recorded using a fluorescence spectrometer, and the amine molecular cluster content of the test sample is determined according to the fluorescence spectrum.

2. A novel amine molecular group detection method according to claim 1, characterized in that: The step of selecting an illumination light source based on the light source determination rule and determining an optimal pulse frequency of the illumination light source comprises the following steps: S11, selecting an illumination light source from candidate light sources based on a light source determination rule; S12, setting the excitation wavelength range according to the absorption peak, and determining the excitation wavelength based on the wavelength constraint rule; S13. Determine the optimal pulse frequency of the irradiation light source based on the excitation wavelength and target characteristics.

3. A novel amine molecular group detection method according to claim 2, characterized in that: The step of selecting an illumination light source from among the candidate light sources based on the light source determination rule comprises the following steps: S111, based on each candidate light source, measuring the excitation wavelength and emission wavelength of the fluorescent substance, and determining the intensity of the fluorescence effect according to the fluorescence spectrum of the fluorescent substance; S112. According to the measurement results, if the excitation wavelength of the fluorescent substance is always within the excitation wavelength range of the amine reactant in the fluorescent reaction chamber, the score of the corresponding candidate light source is increased by 1; S113. According to the analysis results, if the fluorescence intensity of the amine fluorescent substance remains unchanged for more than half of the measurement time, the score of the corresponding candidate light source is increased by 1; S114. Count the scores of all candidate light sources, and select the candidate light source with the highest score as the illumination light source.

4. A novel amine molecular group detection method according to claim 2, characterized in that: The excitation wavelength range set according to the absorption peak is 490-570nm; The wavelength constraint rule is: the excitation wavelength is shorter than the filtering wavelength of the long-pass filter.

5. A novel amine molecular group detection method according to claim 2, characterized in that: Determining the optimal pulse frequency of the irradiation light source based on the excitation wavelength and the fluorescence signal characteristics comprises the following steps: S131, performing an initial experiment based on the irradiation light source, and recording the initial response of the fluorescent substance to different pulse frequencies; S132, gradually increasing the pulse frequency of the irradiation light source, and recording the intensity of the fluorescence signal; S133. When the fluorescence signal intensity is stable and the acquisition signal rate is stable, the pulse frequency at this time is selected as the optimal pulse frequency.

6. A novel method for detecting amine molecular groups according to claim 1, characterized in that: The method of irradiating the detection sample with the filtered light, controlling the irradiation light source to turn off, and collecting the fluorescence signal of the detection sample comprises the following steps: S31, irradiating the test sample with the light filtered by the filter unit to cause the amine molecular clusters to produce a fluorescent effect; S32, optimizing the reaction conditions and controlling the duty cycle of the laser light source to ensure the stability of fluorescence signal collection; S33, turn off the illumination light source, and collect the fluorescence signal of the amine molecular cluster in the smooth mirror channel at the long-pass filter.

7. A novel method for detecting amine molecular groups according to claim 6, characterized in that: The optimization of reaction conditions and control of the duty cycle of the laser light source to ensure the stability of fluorescence signal collection include the following steps: S321, adjusting the pH value and concentration of the reactants by adding a buffer; S322, using a temperature controller to control the temperature in the fluorescence reaction chamber; S323, adjusting the duty cycle of the excitation light source to match the stable collection of the fluorescence signal.

8. A novel amine molecular group detection device, used to implement the novel amine molecular group detection method according to any one of claims 1 to 7, characterized in that: The novel amine molecular group detection device comprises: an irradiation light source, a reflector, a filtering unit, an optical fiber, a smooth mirror channel, a photomultiplier tube, a control unit and a data analysis unit; Wherein, the irradiation light source is used to emit laser light of a specific frequency; The reflector, filter unit and optical fiber are used for filtering, focusing and transmitting the light emitted by the irradiation light source; The filtering unit includes a condenser, a monochromatic filter and a long-pass filter, which are used to filter the light emitted by the irradiation light source; The smooth mirror channel is used to assist the laser irradiation detection sample and the reflection of the fluorescence signal; The photomultiplier tube is used to measure the fluorescence signal of the detection sample and record the change of the fluorescence signal; The control unit is used to control the pulse frequency and duty cycle of the irradiation light source, and to receive and process the fluorescence signal data measured by the photomultiplier tube; The data analysis unit is used to determine the content of amine molecular groups in the test sample according to the change of the fluorescent signal.

9. A novel amine molecular group detection device according to claim 8, characterized in that: The method of judging the content of amine molecular groups in the test sample according to the change of the fluorescent signal includes: Prepare a series of amine standard solutions of known concentrations; Based on the amine standard solution, a standard curve diagram between the fluorescence signal and the standard concentration of the amine is drawn, wherein the X-axis of the standard curve diagram is the concentration of the amine in the standard solution, and the Y-axis is the corresponding fluorescence signal value; Based on the test sample, a test curve diagram between the fluorescence signal and the concentration of the amine molecular group is drawn, wherein the X-axis of the test curve diagram is the concentration of the amine molecular group in the test sample, and the Y-axis is the corresponding fluorescence signal value; The measured test curve graph is compared with the standard curve graph, and the content of amine molecular groups in the test sample is determined.

10. A novel amine molecular group detection device according to claim 9, characterized in that: The step of comparing the measured detection curve with the standard curve and determining the content of amine molecular groups in the detection sample comprises: Compare the test curve with the standard curve. If the fluorescence signal value of the test sample is close to a point in the standard curve, it is determined that amine molecular clusters exist in the test sample, and the content of amine molecular clusters is estimated based on the corresponding point in the standard curve. If the fluorescence signal value of the test sample is much lower than the lowest point in the standard curve, it is judged that there is no or only a very small amount of amine molecular clusters in the test sample.

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