Diagnosis method and detection system based on autoimmune disease antibody detection

Abstract

The invention relates to the technical field of antibody detection methods, in particular to a diagnosis method and a detection system based on autoimmune disease antibody detection, and the method comprises the following steps: S1, an analysis module obtains electrochemistry related information and light reflection related information through electrochemical analysis and light reflection analysis; related information of electrochemistry and related information of light reflection are transmitted to the control module; s2, the control module obtains an antibody concentration index according to the electrochemistry related information and the light reflection related information, and transmits the antibody concentration index to a diagnosis module; s3, the diagnosis module obtains strong or weak immunoreaction information according to the antibody concentration index, and transmits the strong or weak immunoreaction information to the communication module; and S4, the communication module transmits strong or weak immunoreaction information to the user side. Immunoassay is combined with electrochemical analysis and light reflection analysis for detection, and the accuracy and comprehensiveness of detection can be improved by analyzing in different directions and combining results.

Description

Technical Field

[0001] The present invention relates to the technical field of antibody detection methods, and in particular to a diagnostic method and a detection system based on autoimmune disease antibody detection. Background Art

[0002] Autoimmune disease antibody testing is a method of diagnosing autoimmune diseases by detecting specific antibodies in a person's blood. In these diseases, the immune system mistakenly attacks its own healthy cells and tissues, producing antibodies that bind to self-antigens (i.e., self-cells or molecules). By detecting the presence and concentration of these antibodies, it can help identify and diagnose conditions such as systemic lupus erythematosus, rheumatoid arthritis, and thyroid autoimmune diseases. The detection of these antibodies usually uses immunological techniques such as enzyme-linked immunosorbent assay (ELISA), immunofluorescence or chemiluminescent immunoassay to accurately identify and quantify antibodies, thereby providing a basis for early diagnosis, monitoring and treatment of the disease.

[0003] The application document with publication number CN112114135A discloses an autoimmune detection system, which belongs to the field of medical device technology. It solves the problem that the prior art requires medical staff to continuously load materials on multiple devices, and the working hours are long and the work intensity is high. The present invention includes a fluorescent immunoassay analyzer, a first immunoblot instrument, an enzyme-free workstation and a test tube substrate, and also includes a feeding device, a conveying mechanism and a control device for controlling the feeding device and the conveying mechanism. The feeding device is respectively connected to the fluorescent immunoassay analyzer, the first immunoblot instrument and the enzyme-free workstation, and the feeding device is used to complete the feeding and unloading of the test tube substrate. Several manipulators, the manipulators can respectively send the test tube substrate into the fluorescent immunoassay analyzer, the first immunoblot instrument and the enzyme-free workstation to complete the feeding operation.

[0004] The detection method in the prior art is relatively simple, resulting in large errors in the detection results. Summary of the invention

[0005] The purpose of the present invention is to address the above-mentioned deficiencies and to provide a diagnostic method and detection system based on autoimmune disease antibody detection.

[0006] The present invention adopts the following technical solution: A diagnostic method based on autoimmune disease antibody detection, the method comprising the following steps: S1: the analysis module obtains electrochemical related information and light reflection related information through electrochemical analysis and light reflection analysis, and transmits the electrochemical related information and light reflection related information to the control module; S2: the control module obtains an antibody concentration index based on the electrochemical related information and light reflection related information, and transmits the antibody concentration index to the diagnosis module; S3: the diagnosis module obtains information on whether the immune response is strong or weak based on the antibody concentration index, and transmits the information on whether the immune response is strong or weak to the communication module; S4: the communication module transmits the information on whether the immune response is strong or weak to the user end.

[0007] The present invention also provides an autoimmune disease antibody detection system, which is applied to a diagnostic method based on autoimmune disease antibody detection, and the system includes the analysis module, control module, diagnosis module and communication module; the analysis module is used to analyze and obtain electrochemical related information and light reflection related information, and transmit the electrochemical related information and light reflection related information to the control module; the control module obtains an antibody concentration index based on the electrochemical related information and light reflection related information, and transmits the antibody concentration index to the diagnosis module; the diagnosis module obtains information on whether the immune response is strong or weak based on the antibody concentration index, and transmits the information on whether the immune response is strong or weak to the communication module; the communication module transmits the information on whether the immune response is strong or weak to the user end.

[0008] Optionally, the analysis module includes an electrochemical analysis submodule, a light reflection analysis submodule and an affinity analysis submodule; the electrochemical analysis submodule is used to analyze and obtain the bias current, the current before antigen-antibody binding and the current after antigen-antibody binding, and transmit them to the control module; the light reflection analysis submodule is used to analyze and obtain the incident light intensity, the reflected light intensity, the wavelength of the incident light, the refractive index before antigen-antibody binding and the refractive index after antigen-antibody binding, and transmit them to the control module; the affinity analysis submodule is used to analyze and obtain the forward rate constant of antigen-antibody binding and the rate constant of antigen-antibody dissociation, and transmit them to the control module; the control module The forward rate factor of antigen-antibody binding is obtained based on the forward rate constant of antigen-antibody binding, the refractive index change factor is obtained based on the refractive index before antigen-antibody binding and the refractive index after antigen-antibody binding, the light attenuation factor is obtained based on the refractive index change factor and the wavelength of the incident light, the antigen-antibody binding affinity factor is obtained based on the forward rate factor of antigen-antibody binding and the rate constant of antigen-antibody dissociation, the current signal factor is obtained based on the current before antigen-antibody binding and the current after antigen-antibody binding, and the antibody concentration index is obtained based on the current signal factor, bias current, antigen-antibody binding affinity factor, incident light intensity, reflected light intensity and light attenuation factor.

[0009] Optionally, the light reflection analysis submodule includes a light intensity analysis unit, a wavelength storage unit and a refractive index analysis unit; the light intensity analysis unit is used to analyze and obtain the intensity of incident light and the intensity of reflected light, and transmit them to the control module; the wavelength storage unit is used to analyze and obtain the wavelength of incident light, and transmit it to the control module; the refractive index analysis unit is used to analyze and obtain the refractive index before antigen-antibody binding and the refractive index after antigen-antibody binding, and transmit it to the control module.

[0010] Optionally, when the control module calculates the antibody concentration index, the following formula is satisfied: ;in, is an indicator of antibody concentration. is the current signal factor, is the bias current, It is the antigen-antibody binding affinity factor. is the incident light intensity, is the reflected light intensity, is the light attenuation factor.

[0011] The beneficial effects achieved by the present invention are: Combining immunoassay with electrochemical analysis and light reflection analysis, analyzing in different directions and combining the results can improve the accuracy and comprehensiveness of the test; The system adopts a modular design, and each submodule (such as electrochemical analysis submodule, light reflectance analysis submodule, etc.) can be independently upgraded or adjusted as needed, with good scalability and flexibility.

[0012] To further understand the features and technical contents of the present invention, please refer to the following detailed description and drawings of the present invention. However, the drawings provided are only for reference and description and are not intended to limit the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 A method flow chart of a diagnostic method based on autoimmune disease antibody detection of the present invention; Figure 2 This is a schematic diagram of the overall structure of an autoimmune disease antibody detection system according to the present invention; Figure 3 This is a schematic diagram of the structure of a light reflection analysis submodule in an autoimmune disease antibody detection system according to the present invention; Figure 4 This is a rendering of an autoimmune disease antibody detection system according to the present invention; Figure 5 This is a schematic diagram of the overall structure of Embodiment 2 of the autoimmune disease antibody detection system of the present invention; Figure 6This is a schematic diagram of the structure of the environmental analysis submodule in Example 2 of the autoimmune disease antibody detection system of the present invention. DETAILED DESCRIPTION

[0014] The following is an explanation of the embodiments of the present invention through specific embodiments. Those skilled in the art can understand the advantages and effects of the present invention from the contents disclosed in this specification. The present invention can be implemented or applied through other different specific embodiments, and the details in this specification can also be modified and changed in various ways based on different viewpoints and applications without departing from the spirit of the present invention. In addition, the drawings of the present invention are only simple schematic illustrations and are not depicted according to actual dimensions. It is stated in advance. The following embodiments will further explain the relevant technical contents of the present invention in detail, but the disclosed contents are not intended to limit the scope of protection of the present invention.

[0015] Combination Figures 1 to 4 shown.

[0016] Embodiment 1: This embodiment provides a diagnostic method based on the detection of autoimmune disease antibodies.

[0017] A diagnostic method based on autoimmune disease antibody detection, the method comprising the following steps: S1: the analysis module obtains electrochemical related information and light reflection related information through electrochemical analysis and light reflection analysis, and transmits the electrochemical related information and light reflection related information to the control module; S2: the control module obtains an antibody concentration index based on the electrochemical related information and light reflection related information, and transmits the antibody concentration index to the diagnosis module; S3: the diagnosis module obtains information on whether the immune response is strong or weak based on the antibody concentration index, and transmits the information on whether the immune response is strong or weak to the communication module; S4: the communication module transmits the information on whether the immune response is strong or weak to the user end.

[0018] Specifically, when the diagnostic module analyzes, refer to the following principles: when the antibody concentration index is greater than or equal to the selection threshold of the antibody concentration index, it indicates a strong immune response; when the antibody concentration index is less than the selection threshold of the antibody concentration index, it indicates a weak immune response; the selection threshold of the antibody concentration index is set by a technician in this field. It is understandable that autoimmune disease refers to the immune system mistakenly attacking its own tissues. In this case, a strong immune response does not mean that there is an external pathogen, but rather an erroneous response of the internal immune system. For example, in diseases such as rheumatoid arthritis, lupus erythematosus, and multiple sclerosis, abnormal activation of the immune system will lead to a strong immune response, attacking the body's own tissues, and causing the appearance of the disease. In this case, the manifestation of a strong immune response is part of the disease.

[0019] This embodiment solves the problem that the traditional diagnostic method is relatively single, and combines immunoassay with electrochemical analysis and light reflection analysis for detection. Analyzing in different directions and combining the results can improve the accuracy and comprehensiveness of the detection.

[0020] The present embodiment also provides an autoimmune disease antibody detection system, which is applied to a diagnostic method based on autoimmune disease antibody detection, and the system includes the analysis module, control module, diagnosis module and communication module; the analysis module is used to analyze and obtain electrochemical related information and light reflection related information, and transmit the electrochemical related information and light reflection related information to the control module; the control module obtains an antibody concentration index based on the electrochemical related information and light reflection related information, and transmits the antibody concentration index to the diagnosis module; the diagnosis module obtains information on whether the immune response is strong or weak based on the antibody concentration index, and transmits the information on whether the immune response is strong or weak to the communication module; the communication module transmits the information on whether the immune response is strong or weak to the user end.

[0021] Optionally, the analysis module includes an electrochemical analysis submodule, a light reflection analysis submodule and an affinity analysis submodule; the electrochemical analysis submodule is used to analyze and obtain the bias current, the current before antigen-antibody binding and the current after antigen-antibody binding, and transmit them to the control module; the light reflection analysis submodule is used to analyze and obtain the incident light intensity, the reflected light intensity, the wavelength of the incident light, the refractive index before antigen-antibody binding and the refractive index after antigen-antibody binding, and transmit them to the control module; the affinity analysis submodule is used to analyze and obtain the forward rate constant of antigen-antibody binding and the rate constant of antigen-antibody dissociation, and transmit them to the control module; the control module The forward rate factor of antigen-antibody binding is obtained based on the forward rate constant of antigen-antibody binding, the refractive index change factor is obtained based on the refractive index before antigen-antibody binding and the refractive index after antigen-antibody binding, the light attenuation factor is obtained based on the refractive index change factor and the wavelength of the incident light, the antigen-antibody binding affinity factor is obtained based on the forward rate factor of antigen-antibody binding and the rate constant of antigen-antibody dissociation, the current signal factor is obtained based on the current before antigen-antibody binding and the current after antigen-antibody binding, and the antibody concentration index is obtained based on the current signal factor, bias current, antigen-antibody binding affinity factor, incident light intensity, reflected light intensity and light attenuation factor.

[0022] Optionally, the light reflection analysis submodule includes a light intensity analysis unit, a wavelength storage unit and a refractive index analysis unit; the light intensity analysis unit is used to analyze and obtain the intensity of incident light and the intensity of reflected light, and transmit them to the control module; the wavelength storage unit is used to analyze and obtain the wavelength of incident light, and transmit it to the control module; the refractive index analysis unit is used to analyze and obtain the refractive index before antigen-antibody binding and the refractive index after antigen-antibody binding, and transmit it to the control module.

[0023] Optionally, when the control module calculates the antibody concentration index, the following formula is satisfied: ;in, is an indicator of antibody concentration. is the current signal factor, is the bias current, It is the antigen-antibody binding affinity factor. is the incident light intensity, is the reflected light intensity, is the light attenuation factor.

[0024] Optionally, the control module satisfies the following formula when calculating: ; ; ; .

[0025] in, is the current before antigen-antibody binding, is the current after antigen-antibody binding; is the forward rate factor for antigen-antibody binding, is the rate constant of antigen-antibody dissociation; is the refractive index variation factor, is the wavelength of the incident light; is the refractive index before antigen-antibody binding, is the refractive index after antigen-antibody binding.

[0026] When the control module calculates the antibody concentration index, refer to the following program code: import math # Define input parameters # ΔI: Current signal factor delta_I = float(input("Please enter ΔI (current signal factor):")) # b: bias current b = float(input("Please enter b (bias current):")) # K: Antigen-antibody binding affinity factor KF = float(input("Please enter KF (forward rate factor of antigen-antibody binding):")) kd = float(input("Please enter kd (the rate constant for antigen-antibody dissociation):")) K = KF / kd # Calculate K # rs: incident light intensity rs = float(input("Please enter rs (incident light intensity):")) # fs: reflected light intensity fs = float(input("Please enter fs (reflected light intensity):")) # λ: wavelength of incident light lambda_val = float(input("Please enter λ (incident light wavelength, unit nm):")) # Refractive index change ΔN n_before = float(input("Please enter n_before (refractive index before antigen-antibody binding):")) n_after = float(input("Please enter n_after (refractive index after antigen-antibody binding):")) delta_N = n_before - n_after # Calculate α (light attenuation factor) alpha = (4 * math.pi * delta_N) / lambda_val # Calculate the antibody concentration index C C = 0.7 * ((delta_I - b) / (5 * K)) + 0.3 * (-math.log(1 + (fs / rs)) / (alpha * K)) # Output results print(f"The calculation result of antibody concentration index C is: {C}") Specifically, electrochemical analysis and light reflectance analysis experiments are needed to calculate the antibody concentration index. Electrochemical analysis uses the relationship between the current signal and the antibody concentration to infer the antibody level, while light reflectance analysis uses the relationship between the reflected light intensity and the antibody concentration to infer the antibody level.

[0027] The unit of bias current is microampere. Bias current refers to the initial current value detected in the absence of antibodies and antigens. It is usually caused by the inherent noise of the sensor, the measurement circuit or environmental factors.

[0028] The incident light intensity refers to the initial light intensity detected in the absence of antibodies and antigens.

[0029] The reflected light intensity refers to the reflected light intensity after antigen-antibody binding.

[0030] The forward rate constant of antigen-antibody binding and the rate constant of antigen-antibody dissociation can be obtained through SPR experiments (in SPR experiments, light is incident on a metal surface (usually gold or silver) and generates surface plasmon waves. When an antigen or antibody binds to a molecule on the metal surface, it causes a change in the surface refractive index. This change in refractive index affects the reflection angle of the incident light, and the change in reflection angle can be used to quantitatively analyze the binding and dissociation processes). The forward rate constant of antigen-antibody binding describes the binding rate between the antigen and the antibody, and the rate constant of antigen-antibody dissociation describes the rate at which the antigen and the antibody separate after the complex is formed; the unit of the forward rate factor of antigen-antibody binding is milliliter / microgram·minute, and the unit of the rate constant of antigen-antibody dissociation is per minute; in this embodiment, the forward rate factor of antigen-antibody binding is equivalent to the forward rate constant of antigen-antibody binding obtained by direct detection.

[0031] The wavelength of the incident light is measured in nanometers.

[0032] The refractive index before and after antigen-antibody binding can be obtained through SPR experiments (in SPR experiments, incident light is irradiated to the metal surface through an optical fiber or lens system, and surface plasmon waves are generated on the metal surface. The change in refractive index affects the reflection angle, so the change in refractive index can be inferred from the reflection angle).

[0033] The above units are only examples, and those skilled in the art may set different units according to actual needs when implementing this solution.

[0034] This embodiment solves the problem of low detection flexibility of traditional detection systems. The system adopts a modular design, and each submodule (such as electrochemical analysis submodule, light reflection analysis submodule, etc.) can be independently upgraded or adjusted as needed, with good scalability and flexibility.

[0035] Embodiment 2: This embodiment includes all the contents of embodiment 1, and provides an autoimmune disease antibody detection system, combined with Figure 5 and Figure 6 shown.

[0036] An autoimmune disease antibody detection system, wherein the analysis module also includes an environmental analysis submodule; The environment analysis submodule is used to analyze and obtain relevant information about the environment, and transmit the relevant information about the environment to the control module; The control module obtains the forward rate factor of antigen-antibody binding according to relevant information of the environment and the forward rate constant of antigen-antibody binding.

[0037] Optionally, the environmental analysis submodule includes a temperature analysis unit, a humidity analysis unit and a pH analysis unit; the temperature analysis unit is used to analyze and obtain the measured value of the ambient temperature and the ideal value of the ambient temperature, and transmit them to the control module; the humidity analysis unit is used to analyze and obtain the measured value of the ambient humidity and the ideal value of the ambient humidity, and transmit them to the control module; the pH analysis unit is used to analyze and obtain the measured value of the pH of the solution, the first ideal value of the pH of the solution and the second ideal value of the pH of the solution, and transmit them to the control module; the control module obtains the pH index of the solution according to the measured value of the pH of the solution, the first ideal value of the pH of the solution and the second ideal value of the pH of the solution, and obtains the forward rate factor of the antigen-antibody binding according to the pH index of the solution, the forward rate constant of antigen-antibody binding, the measured value of the ambient temperature, the ideal value of the ambient temperature, the measured value of the ambient humidity and the ideal value of the ambient humidity.

[0038] Optionally, when the control module calculates the forward rate factor of antigen-antibody binding, the following formula is satisfied: ; .

[0039] in, is the forward rate constant for antigen-antibody binding, is the measured value of the ambient temperature, is the ideal value of ambient temperature, is the measured value of ambient humidity, is the ideal value of ambient humidity, It is an indicator of the pH value of the solution; is the measured value of the solution pH, is the first ideal value of solution pH, It is the second ideal value of solution pH.

[0040] When the control module calculates the forward rate factor of antigen-antibody binding, refer to the following program code: # Define input parameters # Ambient temperature and humidity related parameters temp_ec = float(input("Please enter temp_ec (measured value of ambient temperature, unit: ℃):")) temp_ref = float(input("Please enter temp_ref (ideal value of ambient temperature, unit: ℃):")) sd_ec = float(input("Please enter sd_ec (measured value of ambient humidity, unit: %)):")) sd_ref = float(input("Please enter sd_ref (ideal value of ambient humidity, unit: %)):")) # Solution pH related parameters ph_ec = float(input("Please enter ph_ec (measured value of solution pH):")) ph1 = float(input("Please enter ph1 (the first ideal value of solution pH):")) ph2 = float(input("Please enter ph2 (the second ideal value of solution pH):")) # Forward rate constant correlation k_f = float(input("Please enter k_f (forward rate constant for antigen-antibody binding, unit: mL / μg·min):")) # Define the calculation of solution pH factor SJ if ph1 <= ph_ec <= ph2: SJ = 1 # In the ideal range, the pH factor is 1 else: SJ = 1.1 # Not within the ideal range, pH factor is 1.1 # Calculate the forward rate factor KF of antigen-antibody binding KF = k_f * ((1 - (temp_ec - temp_ref) / temp_ref) * (1 + abs(sd_ec -sd_ref) / (sd_ec + sd_ref)) * SJ) # Output calculation results print(f"The calculation result of the forward rate factor KF of antigen-antibody binding is: {KF}") Specifically, when the accuracy of the experiment is required to be high, the forward rate constant of the antigen-antibody binding needs to be corrected and compensated.

[0041] The forward rate constant for antigen-antibody binding is expressed in milliliters per microgram per minute.

[0042] The units of the measured value of ambient temperature and the ideal value of ambient temperature are both degrees Celsius. The ideal value of ambient temperature is set by technical personnel in this field. When the measured value of ambient temperature is greater than the ideal value of ambient temperature, the temperature increase will increase the kinetic energy of molecules, increase the frequency of collisions between molecules, and accelerate the binding reaction rate, which will lead to a larger forward rate constant for antigen-antibody binding obtained from the test; when the measured value of ambient temperature is less than the ideal value of ambient temperature, low temperature will lead to a decrease in the kinetic energy of molecules, a decrease in the frequency of collisions between antigens and antibodies, and a slower reaction rate, which will lead to a smaller forward rate constant for antigen-antibody binding obtained from the test. The forward rate constant is adjusted under the current ambient temperature conditions so that the antibody concentration index meets the preset range, thereby obtaining a test that meets the current ambient temperature conditions. .

[0043] When the measured value of ambient humidity is greater than or less than the ideal value of ambient humidity, excessive humidity may cause the sensor electrode or antibody surface to absorb water, change the antibody's conformation or its adsorption capacity on the surface, and cause the reaction rate to decrease. Under low humidity, insufficient moisture on the sensor surface or in the reaction medium may cause adverse changes in the antibody-antigen binding site, or reduce the antibody's adsorption capacity and slow the reaction. Both of the above situations will cause the forward rate constant of antigen-antibody binding obtained from the test to be too small. Adjust the forward rate constant under the current ambient humidity conditions so that the antibody concentration index meets the preset range, thereby obtaining a value that meets the current ambient humidity conditions. .

[0044] The first ideal value of the solution pH and the second ideal value of the solution pH are set by those skilled in the art, and the solution refers to the solution after the antigen and antibody are mixed; when the measured value of the solution pH is less than the first ideal value of the solution pH, the amino groups or other charged groups on the surface of the antigen and antibody may be protonated, changing the conformation of the antibody-antigen binding site, resulting in a smaller forward rate constant for antigen-antibody binding obtained by the test; when the measured value of the solution pH is greater than the second ideal value of the solution pH, the amino or carboxyl ion groups of the antigen and antibody will lose their charge, affecting the electrostatic interaction between the antigen and antibody, and the affinity of the antibody may be reduced, thereby affecting the binding rate between the antigen and the antibody, resulting in a smaller forward rate constant for antigen-antibody binding obtained by the test. The forward rate constant is adjusted under the solution pH condition so that the antibody concentration index meets the preset range, thereby obtaining a pH value that meets the current solution pH. .

[0045] After obtaining three forward rate constants satisfying the three conditions according to the three conditions of ambient temperature, ambient humidity and solution pH, the three forward rate constants are divided by three to obtain a final forward rate constant.

[0046] The above units are only examples, and those skilled in the art may set different units according to actual needs when implementing this solution.

[0047] This embodiment solves the problem of low detection accuracy of the traditional detection system by correcting the forward rate constant of antigen-antibody binding, thereby improving the overall detection accuracy.

[0048] The contents disclosed above are only preferred feasible embodiments of the present invention, and do not limit the protection scope of the present invention. Therefore, all equivalent technical changes made using the contents of the present invention description and drawings are included in the protection scope of the present invention. In addition, the elements therein can be updated as technology develops.

Claims

1. A diagnostic method based on the detection of autoimmune disease antibodies, characterized in that: The Method The following steps are included: S1: The analysis module obtains electrochemical related information and light reflection related information through electrochemical analysis and light reflection analysis, and transmits the electrochemical related information and light reflection related information to the control module; S2: The control module obtains an antibody concentration index based on the electrochemical related information and the light reflection related information, and transmits the antibody concentration index to the diagnosis module; S3: The diagnosis module obtains information about whether the immune response is strong or weak based on the antibody concentration index, and transmits the information about whether the immune response is strong or weak to the communication module; S4: The communication module transmits the information of whether the immune response is strong or weak to the user end.

2. A system for detecting antibodies against autoimmune diseases, applied to a diagnostic method for detecting antibodies against autoimmune diseases as claimed in claim 1, characterized in that: The system includes the analysis module, control module, diagnosis module and communication module; The analysis module is used to analyze and obtain electrochemical related information and light reflection related information, and transmit the electrochemical related information and light reflection related information to the control module; The control module obtains an antibody concentration index based on the electrochemical related information and the light reflection related information, and transmits the antibody concentration index to the diagnosis module; The diagnostic module obtains information about whether the immune response is strong or weak based on the antibody concentration index, and transmits the information about whether the immune response is strong or weak to the communication module; The communication module transmits information about whether the immune response is strong or weak to the user end.

3. The autoimmune disease antibody detection system according to claim 2, characterized in that: The analysis module includes an electrochemical analysis submodule, a light reflection analysis submodule and an affinity analysis submodule; The electrochemical analysis submodule is used to analyze and obtain the bias current, the current before antigen-antibody binding and the current after antigen-antibody binding, and transmit them to the control module; The light reflection analysis submodule is used to analyze and obtain the incident light intensity, the reflected light intensity, the wavelength of the incident light, the refractive index before antigen-antibody binding and the refractive index after antigen-antibody binding, and transmit them to the control module; The affinity analysis submodule is used to analyze and obtain the forward rate constant of antigen-antibody binding and the rate constant of antigen-antibody dissociation, and transmit them to the control module; The control module obtains a forward rate factor for antigen-antibody binding according to the forward rate constant for antigen-antibody binding, obtains a refractive index change factor according to the refractive index before and after antigen-antibody binding, obtains a light attenuation factor according to the refractive index change factor and the wavelength of incident light, obtains an antigen-antibody binding affinity factor according to the forward rate factor for antigen-antibody binding and the rate constant for antigen-antibody dissociation, obtains a current signal factor according to the current before and after antigen-antibody binding, and obtains an antibody concentration index according to the current signal factor, the bias current, the antigen-antibody binding affinity factor, the incident light intensity, the reflected light intensity, and the light attenuation factor.

4. The autoimmune disease antibody detection system according to claim 3, characterized in that: The light reflection analysis submodule includes a light intensity analysis unit, a wavelength storage unit and a refractive index analysis unit; The light intensity analysis unit is used to analyze and obtain the incident light intensity and the reflected light intensity, and transmit them to the control module; The wavelength storage unit is used to analyze and obtain the wavelength of the incident light and transmit it to the control module; The refractive index analysis unit is used to analyze and obtain the refractive index before and after the antigen-antibody combination, and transmit the results to the control module.

5. The autoimmune disease antibody detection system according to claim 4, characterized in that: When the control module calculates the antibody concentration index, the following formula is satisfied: ； in, is an indicator of antibody concentration. is the current signal factor, is the bias current, It is the antigen-antibody binding affinity factor. is the incident light intensity, is the reflected light intensity, is the light attenuation factor.

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