Microscopic optical coherence elastography method and system

Abstract

The invention relates to the technical field of optical coherence elastography, in particular to a microscopic optical coherence elastography method and system.The method comprises the steps that a collection trigger signal is generated, a vibration control signal and a position control signal are output at the same time, and a target excitation system corresponding to a to-be-detected sample is determined; triggering the line scanning camera by collecting a trigger signal, collecting a line scanning image of the to-be-tested sample, and adjusting the position of the to-be-tested sample in the sample table so as to place the to-be-tested sample at a target test position; controlling a bending actuator or an ultrasonic transducer to vibrate by using the vibration control signal so as to excite low-frequency shear waves or ultrasonic waves in the sample to be detected; the to-be-detected sample is scanned through the position control signal to obtain propagation information of low-frequency shear waves or ultrasonic waves, and the pixel-level biomechanical properties of the to-be-detected sample are reconstructed by using a near-field full-wave inversion algorithm, so that the microstructure of a deeper tissue can be observed, the imaging speed is relatively high, and the detection performance of small inclusions is improved.

Description

Technical Field

[0001] The present application relates to the technical field of optical coherence elastic imaging, and in particular to a microscopic optical coherence elastic imaging method and system. Background Art

[0002] Optical Coherence Elastography (OCE) is an elastic imaging technology developed based on Optical Coherence Tomography (OCT). The basic principle of OCE technology is to use some kind of mechanical excitation to produce static or dynamic deformation in soft tissue, measure the above deformation through OCT technology, and then use the measured deformation to invert the biomechanical properties of soft tissue. Among them, OCE technology based on static deformation has been initially applied in the problem of determining the boundary of tumor tissue during surgery. The limitation of OCE technology based on static deformation is that it can only qualitatively measure the elastic modulus of soft tissue, while OCE technology based on dynamic deformation can quantitatively give the biomechanical properties of soft tissue and has a wide range of applications.

[0003] Understanding the mechanical properties of cells and tissues is crucial for studying their physiological functions and pathological changes. In situ, non-destructive measurement techniques can be used to perform measurements without damaging biological tissues. By measuring the stress and elastic properties of biological tissues, it can provide an important basis for early diagnosis and precise treatment of diseases; measuring the effects of drugs on the mechanical properties of biological soft tissues can also evaluate the biological activity and potential effects of drugs. The method based on μOCT combined with elastic imaging can excite shear waves or ultrasound in biological tissues, and reconstruct the biomechanical images of soft tissues through image reconstruction modules. Compared with nanoindentation, atomic force microscopy and other technologies, it has higher resolution, faster imaging speed, three-dimensional imaging, and the ability to penetrate and observe the microstructure inside biological tissues. Therefore, it has a wider range of applications, such as targeted drug design and screening, quality evaluation of organoids and oocytes, etc.

[0004] Embryonic development is a complex process that gradually develops from a single fertilized egg into a complete individual with complex morphology and function. During this process, the nature of biomechanics undergoes a significant transformation. Although many key genes and biochemical factors that control the formation of tissues and organs have been identified, these molecular-level discoveries cannot fully explain how the embryo accurately builds tissues and organs with unique physical properties and three-dimensional structures at the microscopic level. The mechanical properties of the actual cells themselves and the extracellular matrix in which they are located play a crucial role in regulating cell fate and the formation of tissue structures during embryonic development.

[0005] Organoids are three-dimensional structures in vitro constructed using stem cell and other technologies, which can simulate the structure and function of real biological tissues. These highly simulated organ models can not only replicate the morphological characteristics of tissues and organs, but also restore their physiological functions to a certain extent. Organoids have become a popular in vitro model due to their human origin, ability to simulate organ development and formation, genomic stability in long-term in vitro expansion, and ability to form a living biobank for high-throughput screening. The high-throughput organoid model can be used to evaluate the efficacy and potential side effects of drugs. The μOCE system can monitor the morphological changes of biological tissues, efficiently image and analyze organoids in three dimensions, and is suitable for imaging studies of organoids. Because various organoids have significant differences in size, morphology, and response to drugs. The application of μOCE in the field of organoids can provide strong support for the histological classification of cancer and personalized medicine.

[0006] However, the above prior art mainly has the following disadvantages: 1. The existing OCT system is affected by the spectral bandwidth and wavelength range, and its resolution is generally around 10-15 μm. Due to the limitation of resolution, it is impossible to observe more subtle structures at the cellular scale; 2. Nanoindentation technology is a contact measurement method, which may cause damage to soft tissues or sensitive materials. Its spatial resolution is limited by the size of the indentation, and it can usually only measure the mechanical properties of the surface or near the surface. It is necessary to test at multiple points to obtain representative data. The test process is time-consuming, and it can usually only measure the mechanical properties of the surface or near the surface. In order to obtain the spatial distribution of mechanical properties; 3. Brillouin microscopy requires more complex equipment and costs, and takes a long time to collect. Moreover, Brillouin can only obtain the longitudinal modulus of soft tissues instead of the shear modulus, and cannot directly reflect the hardness of tissues. The stability and repeatability of imaging are average.

[0007] In summary, the existing micrometer-scale biomechanical measurement technology has low spatial resolution and requires invasive measurements and advance sample preparation, making it difficult to perform dynamic measurements, which urgently needs to be solved. Summary of the invention

[0008] The present application provides a microscopic optical coherence elastic imaging method and system to solve the problems of low spatial resolution of existing micrometer-scale biomechanical measurement technology, the need for invasive measurement and advance sample preparation, and difficulty in dynamic measurement.

[0009] The first aspect of the present application provides a microscopic optical coherence elastic imaging method, comprising the following steps: using a preset multifunctional I / O device to generate an acquisition trigger signal, and simultaneously outputting a vibration control signal and a position control signal; triggering a preset line scan camera through the acquisition trigger signal to acquire a line scan image corresponding to a target sample to be tested, and based on the line scan image, adjusting the position of the target sample to be tested in a preset sample stage so that the target sample to be tested is placed at a target test position; using the vibration control signal to control the vibration of a preset bending actuator or ultrasonic transducer in a target excitation system to excite a low-frequency shear wave or ultrasonic wave in the target sample to be tested at the target test position; based on the position control signal, scanning the target sample to be tested to obtain propagation information of the low-frequency shear wave or the ultrasonic wave, and reconstructing the pixel-level biomechanical properties of the target sample to be tested according to the propagation information and a preset near-field full-wave inversion algorithm.

[0010] Optionally, in one embodiment of the present application, the method of generating an acquisition trigger signal by using a preset multifunctional I / O device and outputting a vibration control signal and a position control signal at the same time includes: determining a target scanning mode and scanning information corresponding to a preset galvanometer, wherein the target scanning mode includes a B mode or an MB mode, and the scanning information includes a scanning length and an offset; based on the target scanning mode and the scanning information, the multifunctional I / O device is enabled to output an X-channel signal and a Y-channel signal, and use the X-channel signal and the Y-channel signal as the position control signal; using the multifunctional I / O device to output a Z-channel waveform, and generating the vibration control signal through the Z-channel waveform; controlling the multifunctional I / O device to generate an acquisition trigger signal that meets a preset frequency requirement through a preset software programming strategy; and detecting whether the target sample to be tested has liquid, and when the target sample to be tested has liquid, selecting a preset underwater excitation system as the target excitation system, and when the target sample to be tested does not have liquid, selecting a preset shear wave excitation system as the target excitation system.

[0011] Optionally, in one embodiment of the present application, the preset line scan camera is triggered by the acquisition trigger signal to acquire a line scan image corresponding to the target sample to be tested, and based on the line scan image, the position of the target sample to be tested in the preset sample stage is adjusted so that the target sample to be tested is placed at the target test position, including: fixing the target sample to be tested in the sample stage, and setting the trigger mode, line acquisition interval, exposure time and data transmission method corresponding to the line scan camera; based on the trigger mode and the acquisition trigger signal, triggering the line scan camera to acquire the line scan image corresponding to the target sample to be tested according to the line acquisition interval, the exposure time and the data transmission method; performing background noise removal and dispersion correction addition operations on the line scan image to obtain a corresponding interference signal, and performing Fourier transform on the interference signal to generate a corresponding A-line diagram; calibrating the position of the target sample to be tested according to the interference signal and the A-line diagram so that the target sample to be tested is placed at the target test position of the sample stage.

[0012] Optionally, in one embodiment of the present application, the vibration control signal is used to control the vibration of a preset bending actuator or ultrasonic transducer in the target excitation system to excite low-frequency shear waves or ultrasonic waves in the target sample to be tested at the target test position, including: when the target excitation system is the shear wave excitation system, a corresponding shear wave waveform signal is generated by the vibration control signal, and the shear wave waveform signal is sent to the multi-functional I / O device to generate a corresponding excitation signal, and the excitation signal is sent to a preset power amplifier to generate a corresponding waveform amplification signal, and the bending actuator is driven by the waveform amplification signal to excite the low-frequency shear wave corresponding to the target sample to be tested; when the target excitation system is the underwater excitation system, the vibration control signal is used to control the ultrasonic transducer to perform high-frequency mechanical vibration to excite the ultrasonic wave corresponding to the target sample to be tested, or, based on the ultrasonic transducer and a preset acoustic metasurface, a needle-shaped Airy beam is generated to excite the ultrasonic wave corresponding to the target sample to be tested through the needle-shaped Airy beam.

[0013] Optionally, in one embodiment of the present application, the target sample to be tested is scanned based on the position control signal to obtain the propagation information of the low-frequency shear wave or the ultrasonic wave, and the pixel-level biomechanical properties of the target sample to be tested are reconstructed according to the propagation information and a preset near-field full-wave inversion algorithm, including: adjusting the scanning direction of the galvanometer by the position control signal to generate a scanning waveform corresponding to the position control signal, and determining the X-direction galvanometer position and the Y-direction galvanometer position corresponding to the position control signal according to the scanning waveform, and controlling the galvanometer to scan the target sample to be tested based on the X-direction galvanometer position and the Y-direction galvanometer position to obtain the propagation information corresponding to the low-frequency shear wave or the ultrasonic wave; performing phase analysis on the propagation information to detect the corresponding full-field shear wave, and performing a full-wave inversion operation on the full-field shear wave based on a preset reverberation shear wave strategy or a SWENet deep neural network model to obtain the corresponding shear modulus and Young's modulus, and reconstructing the pixel-level biomechanical properties of the target sample to be tested.

[0014] A second aspect of the present application provides a microscopic optical coherence elastic imaging system, comprising: a multifunctional I / O device, configured to generate an acquisition trigger signal, and simultaneously output a vibration control signal and a position control signal; a real-time image display module, configured to trigger a preset line scan camera through the acquisition trigger signal to acquire a line scan image corresponding to a target sample to be tested, and based on the line scan image, adjust the position of the target sample to be tested in a preset sample stage so that the target sample to be tested is placed at a target test position; a sample excitation module, configured to control the vibration of a preset bending actuator or ultrasonic transducer in a target excitation system using the vibration control signal, so as to excite a low-frequency shear wave or ultrasonic wave in the target sample to be tested at the target test position; and a reconstruction module, configured to scan the target sample to be tested based on the position control signal, so as to obtain propagation information of the low-frequency shear wave or the ultrasonic wave, and reconstruct the pixel-level biomechanical properties of the target sample to be tested according to the propagation information and a preset near-field full-wave inversion algorithm.

[0015] Optionally, in one embodiment of the present application, the real-time image display module includes: the line scan camera, used to collect the line scan image corresponding to the target sample to be tested, and perform background noise removal and dispersion correction addition operations on the line scan image to obtain a corresponding interference signal, and perform Fourier transform on the interference signal to generate a corresponding A-line diagram, and calibrate the position of the target sample to be tested according to the interference signal and the A-line diagram so that the target sample to be tested is placed at the target test position of the sample stage.

[0016] Optionally, in one embodiment of the present application, the sample excitation module includes: a shear wave excitation unit, which is used to generate a corresponding shear wave waveform signal through the vibration control signal when the target excitation system is the shear wave excitation system, and send the shear wave waveform signal to the multifunctional I / O device to generate a corresponding excitation signal, and send the excitation signal to a preset power amplifier to generate a corresponding waveform amplification signal, and drive the bending actuator through the waveform amplification signal to excite the low-frequency shear wave corresponding to the target sample to be tested; an underwater excitation unit, which is used to set the target test position of the target sample to be tested to the preset sample arm and the focus of the ultrasonic transducer when the target excitation system is the underwater excitation system, and based on the vibration control signal, make the ultrasonic transducer perform high-frequency mechanical vibration to excite the ultrasonic wave corresponding to the target sample to be tested, or, based on the ultrasonic transducer and a preset acoustic metasurface, generate a needle-shaped Airy beam to excite the ultrasonic wave corresponding to the target sample to be tested through the needle-shaped Airy beam.

[0017] Optionally, in one embodiment of the present application, the reconstruction module includes: a galvanometer, used to adjust the scanning direction according to the position control signal to determine the X-direction galvanometer position and the Y-direction galvanometer position corresponding to the position control signal, and to scan the target sample to be tested based on the X-direction galvanometer position and the Y-direction galvanometer position to obtain the propagation information corresponding to the low-frequency shear wave or the ultrasonic wave; a full-wave inversion unit, used to perform phase analysis on the propagation information to detect the corresponding full-field shear wave, and based on a preset reverberation shear wave strategy or a SWENet deep neural network model, perform a full-wave inversion operation on the full-field shear wave to obtain the corresponding shear modulus and Young's modulus, and reconstruct the pixel-level biomechanical properties of the target sample to be tested.

[0018] A third aspect of the present application provides an electronic device, comprising: a memory, a processor, and a computer program stored in the memory and executable on the processor, wherein the processor executes the program to implement the microscopic optical coherence elasticity imaging method as described in the above embodiment.

[0019] Therefore, the embodiments of the present application have the following beneficial effects: The embodiments of the present application can generate an acquisition trigger signal by using a preset multifunctional I / O device, and output a vibration control signal and a position control signal at the same time; trigger a preset line scan camera by the acquisition trigger signal to acquire a line scan image corresponding to the target sample to be tested, and adjust the position of the target sample to be tested in the preset sample stage based on the line scan image, so that the target sample to be tested is placed at the target test position; use the vibration control signal to control the vibration of the preset bending actuator or ultrasonic transducer in the target excitation system to excite low-frequency shear waves or ultrasonic waves in the target sample to be tested at the target test position; scan the target sample to be tested based on the position control signal to obtain the propagation information of the low-frequency shear wave or ultrasonic wave, and reconstruct the pixel-level biomechanical properties of the target sample to be tested according to the propagation information and the preset near-field full-wave inversion algorithm. The present application can realize non-contact measurement, does not change the mechanical properties of the soft tissue itself during measurement, can observe the microstructure of deeper tissues, and has a fast imaging speed, which improves the detection performance of small inclusions. This solves the problems of existing micrometer-scale biomechanical measurement technology, such as low spatial resolution, the need for invasive measurement and advance sample preparation, and difficulty in dynamic measurement.

[0020] Additional aspects and advantages of the present application will be given in part in the description below, and in part will become apparent from the description below, or will be learned through the practice of the present application. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] The above and / or additional aspects and advantages of the present application will become apparent and easily understood from the following description of the embodiments in conjunction with the accompanying drawings, in which: Figure 1 A flowchart of a microscopic optical coherence elastic imaging method provided according to an embodiment of the present application; Figure 2 A graphical user interface data flow processing schematic diagram provided for one embodiment of the present application; Figure 3 A schematic diagram of an X-channel signal and a Y-channel signal output by a multifunctional I / O device provided in one embodiment of the present application; Figure 4 A schematic diagram of a Z channel signal and a pulse signal output by a multifunctional I / O device provided in one embodiment of the present application; Figure 5 A schematic diagram of a shear wave excitation system provided for one embodiment of the present application; Figure 6 A schematic diagram of an underwater excitation system provided for one embodiment of the present application; Figure 7 A schematic diagram of a manufacturing process of an acoustic metasurface provided for one embodiment of the present application; Figure 8A schematic diagram of an acoustic metasurface-based excitation system provided for one embodiment of the present application; Fig. 9 A schematic diagram of a physical information neural network provided for one embodiment of the present application; Fig.10 is an exemplary diagram of a microscopic optical coherence elastic imaging system according to an embodiment of the present application; Fig.11 A schematic diagram of the logical architecture of a microscopic optical coherence elastic imaging system provided for one embodiment of the present application; Fig.12 A schematic diagram of the structure of an electronic device provided in an embodiment of the present application.

[0022] Among them, 10-microscopic optical coherence elastic imaging system; 100-multifunctional I / O device, 200-real-time image display module, 300-sample excitation module, 400-reconstruction module; 1201-memory, 1202-processor, 1203-communication interface. DETAILED DESCRIPTION

[0023] Embodiments of the present application are described in detail below, and examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are intended to be used to explain the present application, and should not be construed as limiting the present application.

[0024] The following describes the microscopic optical coherence elastic imaging method and system of the embodiment of the present application with reference to the accompanying drawings. In response to the problems mentioned in the above background technology, the present application provides a microscopic optical coherence elastic imaging method, in which a collection trigger signal is generated by using a preset multifunctional I / O device, and a vibration control signal and a position control signal are output simultaneously; a preset line scan camera is triggered by the collection trigger signal to collect a line scan image corresponding to the target sample to be tested, and based on the line scan image, the position of the target sample to be tested in the preset sample stage is adjusted so that the target sample to be tested is placed at the target test position; a preset bending actuator or ultrasonic transducer in the target excitation system is controlled to vibrate by a vibration control signal to excite a low-frequency shear wave or ultrasonic wave in the target sample to be tested at the target test position; based on the position control signal, the target sample to be tested is scanned to obtain the propagation information of the low-frequency shear wave or ultrasonic wave, and the pixel-level biomechanical properties of the target sample to be tested are reconstructed according to the propagation information and a preset near-field full-wave inversion algorithm. The present application can realize non-contact measurement, which will not change the mechanical properties of soft tissue itself during measurement, can observe the microstructure of deeper tissue, and has a fast imaging speed, which improves the detection performance of small inclusions. Thus, the existing micrometer-scale biomechanical measurement technology has low spatial resolution, requires invasive measurement and advance sample preparation, and is difficult to perform dynamic measurement.

[0025] Specifically, Figure 1 A flow chart of a microscopic optical coherence elastic imaging method provided in an embodiment of the present application.

[0026] like Figure 1 As shown, the microscopic optical coherence elastic imaging method comprises the following steps: In step S101, a collection trigger signal is generated by using a preset multifunctional I / O device, and a vibration control signal and a position control signal are output simultaneously.

[0027] The embodiments of the present application can firstly control three analog outputs simultaneously through a multi-functional I / O device to generate an acquisition trigger signal, and simultaneously output a vibration control signal and a position control signal. In addition, the embodiments of the present application also need to determine the excitation system corresponding to the sample to be tested, so as to provide reliable data guidance and technical support for the scanning of the sample to be tested.

[0028] Optionally, in one embodiment of the present application, a preset multifunctional I / O device is used to generate an acquisition trigger signal, and a vibration control signal and a position control signal are output simultaneously, including: determining a target scanning mode and scanning information corresponding to a preset galvanometer, wherein the target scanning mode includes a B mode or an MB mode, and the scanning information includes a scanning length and an offset; based on the target scanning mode and the scanning information, the multifunctional I / O device is enabled to output an X-channel signal and a Y-channel signal, and the X-channel signal and the Y-channel signal are used as position control signals; the multifunctional I / O device is used to output a Z-channel waveform, and a vibration control signal is generated through the Z-channel waveform; the multifunctional I / O device is controlled through a preset software programming strategy to generate an acquisition trigger signal that meets a preset frequency requirement; and detecting whether a target sample to be tested has liquid, and when the target sample to be tested has liquid, a preset underwater excitation system is selected as the target excitation system, and when the target sample to be tested does not have liquid, a preset shear wave excitation system is selected as the target excitation system.

[0029] In the actual implementation process, the embodiments of the present application can first determine the target scanning mode (B mode or MB mode) and scanning information (scanning length and offset) corresponding to the galvanometer, and generate a position control signal according to the scanning mode and scanning information.

[0030] In the embodiment of the present application, the multifunctional I / O device can control three analog outputs (X, Y, Z) at the same time, and ensure the synchronization of the system by outputting pulse signals and analog output signals at the same time. Among them, the position control signals in the X and Y directions are used to control the scanning direction of the galvanometer; the signal in the Z direction is used to control the bending actuator, that is, the vibration control signal.

[0031] As a feasible method, the embodiment of the present application can switch to MB mode in the PYTHON code, control the multi-function I / O device to output X, Y channel signals to control the galvanometer position, and output the Z channel waveform to generate a vibration signal. At this time, the scanning galvanometer works synchronously with the PZT, and the system starts MB mode scanning.

[0032] In addition, the embodiments of the present application can also use software programming to control the multi-function I / O device PCIE-6323 to generate 10,000 trigger signals per second to ensure that the camera is triggered once every 100 microseconds.

[0033] Secondly, the embodiments of the present application can also determine whether there is liquid in the sample to be tested. If there is liquid in the sample to be tested, the underwater excitation system can be selected as the target excitation system; if there is no liquid in the sample to be tested, the shear wave excitation system can be selected as the excitation system.

[0034] Therefore, the embodiment of the present application generates an acquisition trigger signal, outputs a vibration control signal and a position control signal at the same time, and determines the corresponding target excitation system, thereby effectively ensuring the smooth execution of the scanning of the sample to be tested.

[0035] In step S102, a preset line scan camera is triggered by an acquisition trigger signal to acquire a line scan image corresponding to the target sample to be tested, and based on the line scan image, the position of the target sample to be tested in the preset sample stage is adjusted to place the target sample to be tested at the target test position.

[0036] Furthermore, the embodiments of the present application also need to trigger the line scan camera by acquiring a trigger signal to acquire a line scan image of the sample to be tested, so as to adjust the position of the sample to be tested in the sample stage through the line scan image, so that the sample to be tested is placed in the target test position.

[0037] Optionally, in one embodiment of the present application, a preset line scan camera is triggered by an acquisition trigger signal to acquire a line scan image corresponding to a target sample to be tested, and based on the line scan image, the position of the target sample to be tested in a preset sample stage is adjusted so that the target sample to be tested is placed at a target test position, including: fixing the target sample to be tested in the sample stage, and setting a trigger mode, line acquisition interval, exposure time and data transmission method corresponding to the line scan camera; triggering the line scan camera based on the trigger mode and the acquisition trigger signal to acquire a line scan image corresponding to the target sample to be tested according to the line acquisition interval, exposure time and data transmission method; performing background noise removal and dispersion correction addition operations on the line scan image to obtain a corresponding interference signal, and performing Fourier transform on the interference signal to generate a corresponding A-line diagram; calibrating the position of the target sample to be tested according to the interference signal and the A-line diagram so that the target sample to be tested is placed at the target test position of the sample stage.

[0038] In the specific implementation process, the biological tissue or material sample to be tested needs to be prepared, and the sample to be tested is fixed on the sample stage of the sample arm to ensure that the sample is stable and easy to image.

[0039] As a feasible method, the line scan camera is selected as Octoplus camera (E2V), which supports software control of its exposure time, data transmission mode and trigger mode, etc. In the microscopic optical coherence tomography system, the line scan camera can be set to external trigger mode. When the exposure time is set to 80 microseconds per line, the acquisition interval between lines is set to about 100 microseconds to ensure that each line can complete exposure, transmission and other tasks.

[0040] Afterwards, in the software setting, the embodiment of the present application acquires 1024 lines each time, and the main memory calls the cache in the camera once. These 1024 lines constitute one frame in the camera, and the frame rate of the camera is about 9.8 frames per second. Then, the embodiment of the present application can start a real-time display graphical user interface (GUI) to collect a line scan image of the sample to be tested through a line scan camera, and perform background noise removal and dispersion correction addition operations on the line scan image to obtain a corresponding interference signal, such as Figure 2 As shown; further, the embodiments of the present application can also perform Fourier transform on the interference signal to generate a corresponding A-line diagram, so as to calibrate the position of the sample to be tested according to the interference signal and the A-line diagram, so that the sample to be tested is placed at the target test position of the sample stage.

[0041] Therefore, the embodiment of the present application calibrates the real-time displayed image and guides the sample to the test position in real time by observing the real-time grayscale image and Fourier transform image; in addition, the imaging speed of the embodiment of the present application is fast, and the grayscale image of the object to be tested can be displayed in the image interface in real time, and there is no need to mark or pre-process the sample, and the surface of the sample to be tested can be quickly found using image guidance.

[0042] In step S103, the vibration control signal is used to control the vibration of a preset bending actuator or ultrasonic transducer in the target excitation system to excite low-frequency shear waves or ultrasonic waves in the target sample to be tested at the target test position.

[0043] Those skilled in the art should understand that the multifunctional I / O device in the embodiment of the present application controls three analog outputs (X, Y, Z) at the same time, and ensures the synchronization of the system by outputting pulse signals and analog output signals at the same time, wherein, Figure 3 The control signals in the X and Y directions are used to control the scanning direction of the galvanometer. Figure 4 The signal in the Z direction shown is used to control the bending actuator or ultrasonic transducer, thereby generating low-frequency shear waves or ultrasonic waves in the sample; when generating the signal, if 1024 waveform points are generated, the value on each waveform point represents the position of the galvanometer and the bending actuator when scanning the 1024 lines of the camera frame.

[0044] It should be noted that when the shear wave excitation system is used for excitation in the embodiment of the present application, the frequency is scanned from 500 Hz to 10 kHz or a single frequency (for example, 1 kHz) is selected; when an underwater excitation system is used for excitation, a focused ultrasonic transducer (frequency range 2 MHz to 50 MHz) is generally used, and alternating acoustic radiation force is excited in the sample through low-frequency (500 Hz to 10 kHz) modulation.

[0045] Optionally, in one embodiment of the present application, a vibration control signal is used to control the vibration of a preset bending actuator or ultrasonic transducer in the target excitation system to excite low-frequency shear waves or ultrasonic waves in the target sample to be tested at the target test position, including: when the target excitation system is a shear wave excitation system, a corresponding shear wave waveform signal is generated by a vibration control signal, and the shear wave waveform signal is sent to a multi-function I / O device to generate a corresponding excitation signal, and the excitation signal is sent to a preset power amplifier to generate a corresponding waveform amplification signal, and the bending actuator is driven by the waveform amplification signal to excite the low-frequency shear wave corresponding to the target sample to be tested; when the target excitation system is an underwater excitation system, the ultrasonic transducer is controlled to perform high-frequency mechanical vibration by a vibration control signal to excite the ultrasonic wave corresponding to the target sample to be tested, or, based on the ultrasonic transducer and a preset acoustic metasurface, a needle-shaped Airy beam is generated to excite the ultrasonic wave corresponding to the target sample to be tested through the needle-shaped Airy beam.

[0046] It should be noted that, in the embodiments of the present application, the waveform of the shear wave can be generated by the software system of the workstation, and the waveform signal is connected to the PZT driver (i.e., the power amplifier), which can be powered by a 30V linear power supply to excite a low-frequency wave (i.e., the excitation signal) with a frequency between 500Hz and 10kHz, and the excitation signal is sent to the power amplifier. The waveform amplification signal generated by the power amplifier is directly connected to the piezoelectric dual-chip bending actuator, and drives the bending actuator to transmit the vibration to the sample on the vibration plate through the transparent vibration plate above it, so as to achieve the effect of dynamic deformation, such as Figure 5 shown.

[0047] It should be noted that, in the actual implementation process, those skilled in the art may also adopt other methods to stimulate low-frequency shear waves in biological soft tissues according to actual conditions, such as some low-frequency mechanical devices, etc., which are not specifically limited here.

[0048] However, Figure 5 Although the shear wave excitation system shown can excite shear waves in solid samples, when there is liquid in the sample being tested, the low-frequency waves generated by the shear wave excitation system cannot be effectively propagated in the liquid.

[0049] Therefore, the present application embodiment also constructs Figure 6 The underwater excitation system shown in Figure 1 is mainly excited by an ultrasonic transducer. This excitation system requires the sample to be placed at the sample arm and the focus of the ultrasonic transducer at the same time. The ultrasonic transducer can convert electrical signals into high-frequency mechanical vibrations. Different from the waves generated by the shear wave excitation system, ultrasonic waves have strong directionality and good penetration, making them suitable for non-invasive detection and precise control.

[0050] As a possible implementation method, the embodiments of the present application can also be implemented as follows: Figure 7 The acoustic metasurface manufacturing process shown constructs an acoustic metasurface to generate a needle-shaped Airy acoustic beam by combining a flat-plate ultrasonic transducer with an acoustic metasurface, so as to excite a shear wave corresponding to a target sample to be tested through the needle-shaped Airy acoustic beam.

[0051] Among them, the flat transducer converts the electrical signal into high-frequency vibration to form ultrasonic waves. The super plane has a special microstructure that can control the propagation of sound waves to form a needle-shaped Airy beam. The focus area of ​​the needle-shaped Airy beam is relatively narrow, which can form a high-intensity sound field at the focus, and produce shear waves with higher amplitude and shorter wavelength in the tissue, thereby increasing the spatial frequency of the shear wave. The Airy beam is self-repairing during propagation and has strong penetrating ability. The Airy needle-shaped Airy beam is not easily affected by diffraction and medium inhomogeneity, and has strong anti-interference ability, so that the imaging system can receive more accurate and higher-quality signals, which is conducive to improving the resolution and signal-to-noise ratio of imaging. Figure 8 Schematic diagram of the excitation system based on acoustic metasurface. Figure 8 As shown, the excitation part includes a single-vibration-source flat piezoelectric chip and a metasurface. When in use, an ultrasonic coupling agent needs to be applied to the metasurface to fill the gap between the excitation part and the sample, reduce the reflection of the sound wave, and enhance the penetration and propagation effect of the sound wave. In the actual implementation process, the self-made flat ultrasonic transducer and acoustic metasurface in the embodiment of the present application can generate ultrasonic waves of various frequencies, and can adjust the phase, amplitude, propagation direction and other information of the ultrasonic wave as needed.

[0052] It can be understood that the ultrasonic waves generated by the underwater excitation system in the embodiment of the present application can propagate well in the liquid and can effectively excite the sample in the liquid.

[0053] In step S104, based on the position control signal, the target sample to be tested is scanned to obtain propagation information of low-frequency shear waves or ultrasonic waves, and the pixel-level biomechanical properties of the target sample to be tested are reconstructed according to the propagation information and a preset near-field full-wave inversion algorithm.

[0054] Furthermore, the embodiments of the present application perform scanning measurements at different positions of the sample based on the position control signal, and perform multiple measurements at different or the same positions of the sample according to actual needs to obtain the propagation information of low-frequency shear waves or ultrasonic waves, and based on the propagation information and the near-field full-wave inversion algorithm, ultimately achieve the characterization of biomechanical properties with a micron (~2 microns) scale resolution, and reconstruct the pixel-level biomechanical properties of the target sample to be tested.

[0055] Therefore, the embodiments of the present application can achieve super-resolution (1.98) by exciting and measuring low-frequency shear waves or ultrasound waves of the target tissue, and by using the spatiotemporal data (especially the velocity) of shear wave and ultrasound propagation, and by using near-field full waves (evanescent waves and diffraction waves, etc.), full-wave inversion can be performed to reconstruct pixel-level biomechanical properties. μ m) biomechanical image; in addition, the embodiments of the present application can realize non-contact measurement, and the mechanical properties of the soft tissue itself will not be changed during measurement. Moreover, the embodiments of the present application can measure the longitudinal modulus of the soft tissue instead of the shear modulus, thereby reflecting the various mechanical properties of the biological tissue.

[0056] Optionally, in one embodiment of the present application, based on the position control signal, the target sample to be tested is scanned to obtain the propagation information of the low-frequency shear wave or ultrasonic wave, and the pixel-level biomechanical properties of the target sample to be tested are reconstructed according to the propagation information and a preset near-field full-wave inversion algorithm, including: adjusting the scanning direction of the galvanometer through the position control signal to generate a scanning waveform corresponding to the position control signal, and determining the X-direction galvanometer position and the Y-direction galvanometer position corresponding to the position control signal according to the scanning waveform, and controlling the galvanometer to scan the target sample to be tested based on the X-direction galvanometer position and the Y-direction galvanometer position to obtain the propagation information corresponding to the low-frequency shear wave or ultrasonic wave; performing phase analysis on the propagation information to detect the corresponding full-field shear wave, and performing a full-wave inversion operation on the full-field shear wave based on the preset reverberation shear wave strategy or SWENet deep neural network model to obtain the corresponding shear modulus and Young's modulus, and reconstructing the pixel-level biomechanical properties of the target sample to be tested.

[0057] In the specific implementation process, the embodiment of the present application can adjust the scanning direction of the galvanometer through a position control signal to generate a scanning waveform corresponding to the position control signal, so as to determine the X-direction galvanometer position and the Y-direction galvanometer position corresponding to the position control signal, thereby controlling the galvanometer to scan the target sample to be tested to obtain the propagation information corresponding to the low-frequency shear wave or ultrasonic wave; secondly, the embodiment of the present application stores signal data such as the propagation information of the current sample and performs phase analysis to detect the full-field shear wave, and then derives the shear modulus and Young's modulus through full-wave inversion, and reconstructs the pixel-level biomechanical properties of the sample to be tested.

[0058] In the actual implementation process, the inversion algorithm in the embodiment of the present application can be implemented by two strategies: the reverberation shear wave method or the machine learning method, as described below: 1. Reverberation Shear Wave Method: The reverberant shear wave method combines OCT and the reverberant shear wave field generated by 1 kHz quasi-harmonic stimulation. Random fluctuations are induced by multiple mechanical actuators, and these fluctuations interfere with each other to form a reverberant shear wave field; the interference data is processed by fast Fourier transform, the axial particle velocity is extracted, and the local reverberant field is used for autocorrelation, and the autocorrelation function is fitted with the theoretical solution to obtain the local wavelength, thereby calculating the shear modulus.

[0059] Therefore, the reverberation shear wave technology in the embodiment of the present application can provide high-resolution three-dimensional elastic imaging with high resolution and high sensitivity, and can accurately observe the biomechanical properties of tiny tissues such as embryos and organoids, which is of great significance for understanding developmental diseases and exploring treatment methods.

[0060] 2. Machine Learning Methods: The embodiment of the present application can also utilize the deep neural network model SWENet based on physical information neural network for shear wave elastography (SWE) image reconstruction, encoding the control equation of the physical problem as a part of the neural network, thereby solving the full-wave inversion problem.

[0061] like Fig. 9 As shown in the figure, the SWENet neural network is a fully connected feedforward neural network, the activation function is the tanh function, and the weights and biases of the network are optimized by the back propagation algorithm; the neural network 1 (NN1) takes the spatial coordinates and time as input, and the function of the wave and Lagrange multipliers as output; Neural Network 2 (NN2) takes the spatial coordinates as input, the shear modulus As output, the two neural networks have slightly different numbers of hidden layers and neurons; the loss function consists of a data-driven part and a physical information part, and the weights of the two parts are controlled by hyperparameters. The data-driven part uses the difference between experimental data and simulation data as the loss function; the physical information part is derived based on the wave equation, boundary conditions, constitutive equations, etc. of elastic waves. These equations and conditions together constitute the mathematical description of the physical system and are introduced into the training process of the neural network in the form of a loss function; when the number of iterations or the loss function converges to a certain extent, the training stops.

[0062] It can be understood that the embodiments of the present application include the spatial and temporal characteristics of fluctuations in the training through the machine learning algorithm, and the multi-source data can be easily integrated with the machine learning algorithm, which can greatly improve the performance in detecting small inclusions and provide a more accurate assessment of the spatial distribution of tissue mechanical properties.

[0063] Therefore, the embodiments of the present application can utilize SWENet to encode the physical properties of elastic waves into a neural network. Compared with other full-wave inversion algorithms (such as the Time of Flight algorithm), the machine learning method can identify more features in the wave propagation process. Through these features, inclusions as small as submillimeter scale inside biological tissues can be better identified, thereby more accurately evaluating the biological characteristics and mechanical properties of tissues.

[0064] According to the microscopic optical coherence elastic imaging method proposed in the embodiment of the present application, a collection trigger signal is generated by using a preset multifunctional I / O device, and a vibration control signal and a position control signal are output simultaneously; a preset line scan camera is triggered by the collection trigger signal to collect a line scan image corresponding to the target sample to be tested, and based on the line scan image, the position of the target sample to be tested in the preset sample stage is adjusted so that the target sample to be tested is placed at the target test position; a preset bending actuator or ultrasonic transducer in the target excitation system is controlled to vibrate by a vibration control signal to excite a low-frequency shear wave or ultrasonic wave in the target sample to be tested at the target test position; based on the position control signal, the target sample to be tested is scanned to obtain the propagation information of the low-frequency shear wave or ultrasonic wave, and the pixel-level biomechanical properties of the target sample to be tested are reconstructed according to the propagation information and the preset near-field full-wave inversion algorithm. The present application can realize non-contact measurement, does not change the mechanical properties of the soft tissue itself during measurement, can observe the microstructure of deeper tissues, and has a fast imaging speed, which improves the detection performance of small inclusions.

[0065] Next, the microscopic optical coherence elastic imaging system proposed according to the embodiment of the present application is described with reference to the accompanying drawings.

[0066] Fig.10 Schematic diagram of a microscopic optical coherence elastic imaging system according to an embodiment of the present application.

[0067] like Fig.10 As shown, the microscopic optical coherence elastic imaging system 10 includes: a multifunctional I / O device 100 , a real-time image display module 200 , a sample excitation module 300 and a reconstruction module 400 .

[0068] The multifunctional I / O device 100 is used to generate an acquisition trigger signal and output a vibration control signal and a position control signal at the same time.

[0069] The real-time image display module 200 is used to trigger a preset line scan camera by acquiring a trigger signal to acquire a line scan image corresponding to the target sample to be tested, and based on the line scan image, adjust the position of the target sample to be tested in a preset sample stage so that the target sample to be tested is placed in the target test position.

[0070] The sample excitation module 300 is used to control the vibration of a preset bending actuator or ultrasonic transducer in the target excitation system by using a vibration control signal, so as to excite low-frequency shear waves or ultrasonic waves in the target sample to be tested at the target test position.

[0071] The reconstruction module 400 is used to scan the target sample to be tested based on the position control signal to obtain the propagation information of the low-frequency shear wave or ultrasonic wave, and reconstruct the pixel-level biomechanical properties of the target sample to be tested according to the propagation information and a preset near-field full-wave inversion algorithm.

[0072] It should be understood by those skilled in the art that the microstructure and biomechanical properties of biological tissues are closely related to the growth, development and pathological changes of biological tissues. Therefore, the embodiments of the present application can be based on microscopic optical coherence tomography (micro optical coherence tomography, μ OCT is used to construct microoptical coherence elastography (microoptical coherence elastography, μ OCE) system is used to characterize the microscopic imaging and biomechanical properties of biological soft tissues such as organoids and embryos, which can then assist in drug screening and egg quality evaluation in in vitro fertilization (IVF).

[0073] It should be noted that the microscopic optical coherence elastic imaging system of the embodiment of the present application mainly includes μ OCT system, excitation system and μ OCE image reconstruction algorithm. μ For OCT systems, the axial resolution is about 1.98 μ m, lateral resolution about 2.1 μ m; The low-frequency wave excitation system based on bending actuator and the liquid ultrasonic excitation system based on ultrasonic transducer can excite low-frequency shear waves and ultrasonic waves in micro-scale animal tissues such as organoids and nematodes and in liquid samples.

[0074] Specifically, the main parts of the microscopic optical coherence elastic imaging system include a superradiant light source, a reference arm, a sample arm, a spectrometer, a line scan camera and a workstation, a frame grabber, a multifunctional I / O device 100 (PCIE-6323, NI), a real-time image display module 200, a sample excitation module 300 and a reconstruction module 400, and the optical components are connected by a 2*2 fiber coupler, such as Fig.11 shown.

[0075] Among them, the reference arm contains a collimation part, a polarization controller, a double cemented lens, a dispersion compensation pair and a plane mirror. The plane mirror is mounted on a translation stage (Thorlabs) to control the optical path difference and sensitivity roll-off; a slit is used to control the signal attenuation degree; the sample arm contains a collimation part, a polarization controller, a scanning galvanometer, an objective lens and a two-dimensional displacement platform (which can carry and adjust the sample position to reach the optimal imaging position).

[0076] The light source uses a super luminescent diode (SLD) light source, which emits light through the recombination of electrons and holes, and uses the principle of combining spontaneous radiation and stimulated radiation to produce wide-spectrum, high-brightness light; compared with supercontinuum light sources, it has lower costs, longer service life, relatively simple structure, stable output, and safe operation, and does not require a strict optical sealing environment. Supercontinuum light sources may cause burns to tissues such as eyes and skin. The SLD light source of the microscopic optical coherence elastic imaging system uses a (cBLMD-T-850-HP-I, SUPERLUM) light source with a central wavelength of 850nm, an output power of 10mw, and a spectrum width of 165nm.

[0077] In an embodiment of the present application, a laser light source can be connected to a 50:50 fiber coupler of Thorlabs to divide the light beam of the light source into a sample arm and a reference arm. After passing through the fiber coupler, the laser enters the collimation parts of the sample arm and the reference arm respectively. The model of the collimator is a double-cemented lens AC-254-030-B. The optical fiber is connected and fixed on a four-dimensional mirror frame of the coaxial system. The collimation part consists of a mirror frame with a double-cemented lens and a four-dimensional adjustable mirror frame with the end of the fiber coupler fixed. The mirror frames are all connected to a coaxial system with adjustable distance. The function of the collimation system is realized by adjusting the four-dimensional mirror frame so that the distance between the end of the optical fiber and the double-cemented lens is equal to the focal length of the double-cemented lens, so that the light passing through the sample arm and the reference arm is parallel light.

[0078] In addition, the objective lens can use AC-127-019-B doublet lens (f=19mm), or the same type of achromatic doublet lens with a focal length of 30mm and 50mm can be used as needed. In the sample arm, the collimated parallel light passes through the galvanometer and the sample; the reflected light of the sample arm interferes with the light reflected from the plane mirror of the reference arm at the coupler, and the interfered light enters the spectrometer.

[0079] Optionally, in one embodiment of the present application, the real-time image display module 200 includes: a line scan camera, used to collect a line scan image corresponding to the target sample to be tested, and perform background noise removal and dispersion correction addition operations on the line scan image to obtain a corresponding interference signal, and perform Fourier transform on the interference signal to generate a corresponding A-line diagram, and calibrate the position of the target sample to be tested according to the interference signal and the A-line diagram so that the target sample to be tested is placed at the target test position of the sample stage.

[0080] It should be noted that the spectrometer in the embodiment of the present application mainly includes a collimator, a plane reflector, a grating, a real-time image display module 200 (including a line scan camera) and a lens group composed of a plurality of lenses.

[0081] The grating uses Wasatch's volume phase holographic grating, the wavelength range of the spectrometer is roughly the same as the light source, the line scan camera used by the spectrometer has a maximum rate of 130kA-scan / s, and a maximum bit depth of 12bit. The actual depth of field of 10bit is used to ensure dual-channel (2taps mode) transmission. The line scan camera has a total of 2048 pixels with a width of 10 microns and a height of 200 microns. The interface connecting the line scan camera to the host is camera-link. The collected data is transmitted using a transmission cable between the line scan camera and the frame grabber. After averaging, background subtraction, dispersion correction, and Fourier transformation, the line scan image of each frame forms an A-line diagram to calibrate the position of the sample to be tested, so that the sample to be tested is placed at the target test position of the sample stage.

[0082] Optionally, in one embodiment of the present application, the sample excitation module 300 includes: a shear wave excitation unit and an underwater excitation unit.

[0083] Among them, the shear wave excitation unit is used to generate a corresponding shear wave waveform signal through a vibration control signal when the target excitation system is a shear wave excitation system, and send the shear wave waveform signal to a multi-functional I / O device to generate a corresponding excitation signal, and send the excitation signal to a preset power amplifier to generate a corresponding waveform amplification signal, and drive the bending actuator through the waveform amplification signal to excite the low-frequency shear wave corresponding to the target sample to be tested.

[0084] The underwater excitation unit is used to set the target test position of the target sample to be tested to the focus of the preset sample arm and the ultrasonic transducer when the target excitation system is an underwater excitation system, and based on the vibration control signal, the ultrasonic transducer performs high-frequency mechanical vibration to excite the ultrasonic wave corresponding to the target sample to be tested, or, based on the ultrasonic transducer and the preset acoustic metasurface, generate a needle-shaped Airy beam to excite the ultrasonic wave corresponding to the target sample to be tested through the needle-shaped Airy beam.

[0085] In the embodiment of the present application, the main components of the sample excitation module 300 include a piezoelectric bimorph bending actuator, a linear power supply, a power amplifier, a PZT driver and a transparent vibrating plate.

[0086] In the specific implementation process, when the target excitation system is a shear wave excitation system, the embodiment of the present application can generate a corresponding shear wave waveform signal through a vibration control signal, and send the shear wave waveform signal to a multi-function I / O device or a PZT driver, which is powered by a 30V linear power supply to generate a corresponding excitation signal, and send the excitation signal to a power amplifier to generate a corresponding waveform amplification signal, and drive the bending actuator through the waveform amplification signal to excite the low-frequency shear wave corresponding to the sample to be tested.

[0087] When the target excitation system is an underwater excitation system, the target test position of the sample to be tested is set to the focus of the sample arm and the ultrasonic transducer, and based on the vibration control signal, the ultrasonic transducer is made to perform high-frequency mechanical vibration to excite ultrasonic waves corresponding to the sample to be tested.

[0088] It should be noted that in the actual implementation process, those skilled in the art may also use light sheet microscopy, fluorescence microscopy and other methods to replace the method used in the embodiment of the present application according to the actual situation. μ OCT is used for the measurement of low-frequency shear waves and ultrasound.

[0089] It can be understood that the microscopic optical coherence elastic imaging system of the embodiment of the present application has an axial resolution of about 1.98 μ m, lateral resolution about 2.1 μ m, more microscopic changes at the cellular scale can be observed, and the use of low-frequency waves in solid samples increases the penetration depth, making it possible to observe the microstructure of deeper tissues.

[0090] Optionally, in one embodiment of the present application, the reconstruction module 400 includes: a galvanometer and a full-wave inversion unit.

[0091] Among them, the galvanometer is used to adjust the scanning direction through the position control signal to determine the X-direction galvanometer position and the Y-direction galvanometer position corresponding to the position control signal, so as to scan the target sample to be tested based on the X-direction galvanometer position and the Y-direction galvanometer position to obtain the propagation information corresponding to the low-frequency shear wave or ultrasonic wave.

[0092] The full-wave inversion unit is used to perform phase analysis on the propagation information to detect the corresponding full-field shear wave, and based on the preset reverberation shear wave strategy or SWENet deep neural network model, perform full-wave inversion operation on the full-field shear wave to obtain the corresponding shear modulus and Young's modulus, and reconstruct the pixel-level biomechanical properties of the target sample to be tested.

[0093] It should be noted that the embodiments of the present application can utilize the galvanometer to adjust the scanning direction through the position control signal to generate a corresponding scanning waveform, determine the X-direction galvanometer position and the Y-direction galvanometer position corresponding to the position control signal, so as to scan the target sample to be tested, thereby obtaining the propagation information corresponding to the low-frequency shear wave or ultrasonic wave; then, the embodiments of the present application can perform phase analysis on the propagation information through the full-wave inversion unit to detect the full-field shear wave, and then obtain the mechanical properties of the biological tissue through full-wave inversion.

[0094] It should be noted that the above explanations of the embodiment of the microscopic optical coherence elastic imaging method are also applicable to the microscopic optical coherence elastic imaging system of this embodiment, and will not be repeated here.

[0095] The microscopic optical coherence elastic imaging system proposed in the embodiment of the present application includes a multifunctional I / O device 100, which is used to generate an acquisition trigger signal and simultaneously output a vibration control signal and a position control signal; a real-time image display module 200, which is used to trigger a preset line scan camera through the acquisition trigger signal to acquire a line scan image corresponding to the target sample to be tested, and adjust the position of the target sample to be tested in a preset sample stage based on the line scan image so that the target sample to be tested is placed at a target test position; a sample excitation module 300, which is used to control the vibration of a preset bending actuator or ultrasonic transducer in a target excitation system using a vibration control signal to excite low-frequency shear waves or ultrasonic waves in the target sample to be tested at the target test position; a reconstruction module 400, which is used to scan the target sample to be tested based on the position control signal to obtain propagation information of the low-frequency shear wave or ultrasonic wave, and reconstruct the pixel-level biomechanical properties of the target sample to be tested according to the propagation information and a preset near-field full-wave inversion algorithm. The present application can realize non-contact measurement, will not change the mechanical properties of the soft tissue itself during measurement, can observe the microstructure of deeper tissues, and has a fast imaging speed, thereby improving the detection performance of small inclusions.

[0096] Fig.12 A schematic diagram of the structure of an electronic device provided in an embodiment of the present application. The electronic device may include: A memory 1201 , a processor 1202 , and a computer program stored in the memory 1201 and executable on the processor 1202 .

[0097] When the processor 1202 executes the program, the microscopic optical coherence elastic imaging method provided in the above embodiment is implemented.

[0098] Furthermore, the electronic device further comprises: The communication interface 1203 is used for communication between the memory 1201 and the processor 1202 .

[0099] The memory 1201 is used to store computer programs that can be executed on the processor 1202 .

[0100] The memory 1201 may include a high-speed RAM memory, and may also include a non-volatile memory (non-volatile memory), such as at least one disk memory.

[0101] If the memory 1201, the processor 1202 and the communication interface 1203 are implemented independently, the communication interface 1203, the memory 1201 and the processor 1202 can be connected to each other through a bus and communicate with each other. The bus can be an Industry Standard Architecture (ISA) bus, a Peripheral Component (PCI) bus or an Extended Industry Standard Architecture (EISA) bus. The bus can be divided into an address bus, a data bus, a control bus, etc. For ease of representation, Fig.12 Only one thick line is used in the diagram, but this does not mean that there is only one bus or only one type of bus.

[0102] Optionally, in a specific implementation, if the memory 1201, the processor 1202 and the communication interface 1203 are integrated on a chip, the memory 1201, the processor 1202 and the communication interface 1203 can communicate with each other through an internal interface.

[0103] The processor 1202 may be a central processing unit (CPU), or an application specific integrated circuit (ASIC), or one or more integrated circuits configured to implement the embodiments of the present application.

[0104] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present application. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or N embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine the different embodiments or examples described in this specification and the features of the different embodiments or examples, without contradiction.

[0105] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Therefore, a feature defined as "first" or "second" may explicitly or implicitly include at least one of the features. In the description of this application, "N" means at least two, such as two, three, etc., unless otherwise clearly and specifically defined.

[0106] Any process or method description in a flowchart or otherwise described herein may be understood to represent a module, fragment or portion of code comprising one or N executable instructions for implementing the steps of a custom logical function or process, and the scope of the preferred embodiments of the present application includes alternative implementations in which functions may not be performed in the order shown or discussed, including performing functions in a substantially simultaneous manner or in reverse order depending on the functions involved, which should be understood by technicians in the technical field to which the embodiments of the present application belong.

Claims

1. A microscopic optical coherence elastic imaging method, characterized in that: The following steps are involved: Use the preset multi-function I / O device to generate acquisition trigger signals and output vibration control signals and position control signals at the same time; Triggering a preset line scan camera through the acquisition trigger signal to acquire a line scan image corresponding to the target sample to be tested, and adjusting the position of the target sample to be tested in a preset sample stage based on the line scan image so that the target sample to be tested is placed at a target test position; Using the vibration control signal to control the vibration of a preset bending actuator or ultrasonic transducer in the target excitation system to excite low-frequency shear waves or ultrasonic waves in the target sample to be tested at the target test position; Based on the position control signal, the target sample to be tested is scanned to obtain the propagation information of the low-frequency shear wave or the ultrasonic wave, and the pixel-level biomechanical properties of the target sample to be tested are reconstructed according to the propagation information and a preset near-field full-wave inversion algorithm.

2. The microscopic optical coherence elastic imaging method according to claim 1, characterized in that: The method of using a preset multifunctional I / O device to generate an acquisition trigger signal and simultaneously output a vibration control signal and a position control signal includes: Determine a target scanning mode and scanning information corresponding to a preset galvanometer, wherein the target scanning mode includes a B mode or an MB mode, and the scanning information includes a scanning length and an offset; Based on the target scanning mode and the scanning information, the multifunctional I / O device outputs an X-channel signal and a Y-channel signal, and uses the X-channel signal and the Y-channel signal as the position control signal; Outputting a Z-channel waveform using the multifunctional I / O device, and generating the vibration control signal through the Z-channel waveform; Controlling the multifunctional I / O device to generate an acquisition trigger signal that meets a preset frequency requirement through a preset software programming strategy; Detect whether there is liquid in the target sample to be tested. When liquid is present in the target sample to be tested, select a preset underwater excitation system as the target excitation system. When liquid is not present in the target sample to be tested, select a preset shear wave excitation system as the target excitation system.

3. The microscopic optical coherence elastic imaging method according to claim 1, characterized in that: The method triggers a preset line scan camera by the acquisition trigger signal to acquire a line scan image corresponding to the target sample to be tested, and adjusts the position of the target sample to be tested in a preset sample stage based on the line scan image so that the target sample to be tested is placed at a target test position, including: Fixing the target sample to be tested in the sample stage, and setting the trigger mode, line acquisition interval, exposure time and data transmission mode corresponding to the line scanning camera; Based on the trigger mode and the acquisition trigger signal, trigger the line scan camera to acquire a line scan image corresponding to the target sample according to the line acquisition interval, the exposure time and the data transmission method; Performing background noise removal and dispersion correction addition operations on the line scan image to obtain a corresponding interference signal, and performing Fourier transform on the interference signal to generate a corresponding A-line graph; The position of the target sample to be tested is calibrated according to the interference signal and the A-line diagram, so that the target sample to be tested is placed at the target test position of the sample stage.

4. The microscopic optical coherence elastic imaging method according to claim 2, characterized in that: The method of using the vibration control signal to control the vibration of a preset bending actuator or ultrasonic transducer in the target excitation system to excite a low-frequency shear wave or ultrasonic wave in the target sample to be tested at the target test position comprises: When the target excitation system is the shear wave excitation system, a corresponding shear wave waveform signal is generated by the vibration control signal, and the shear wave waveform signal is sent to the multifunctional I / O device to generate a corresponding excitation signal, and the excitation signal is sent to a preset power amplifier to generate a corresponding waveform amplification signal, and the bending actuator is driven by the waveform amplification signal to excite a low-frequency shear wave corresponding to the target sample to be tested; When the target excitation system is the underwater excitation system, the vibration control signal is used to control the ultrasonic transducer to perform high-frequency mechanical vibration to excite the ultrasonic wave corresponding to the target sample to be tested, or, based on the ultrasonic transducer and a preset acoustic metasurface, a needle-shaped Airy beam is generated to excite the ultrasonic wave corresponding to the target sample to be tested through the needle-shaped Airy beam.

5. The microscopic optical coherence elastic imaging method according to claim 4, characterized in that: The method of scanning the target sample to be tested based on the position control signal to obtain the propagation information of the low-frequency shear wave or the ultrasonic wave, and reconstructing the pixel-level biomechanical properties of the target sample to be tested according to the propagation information and a preset near-field full-wave inversion algorithm, includes: The scanning direction of the galvanometer is adjusted by the position control signal to generate a scanning waveform corresponding to the position control signal, and the X-direction galvanometer position and the Y-direction galvanometer position corresponding to the position control signal are determined according to the scanning waveform, so as to control the galvanometer to scan the target sample to be tested based on the X-direction galvanometer position and the Y-direction galvanometer position to obtain the propagation information corresponding to the low-frequency shear wave or the ultrasonic wave; The propagation information is phase analyzed to detect the corresponding full-field shear wave, and based on the preset reverberation shear wave strategy or SWENet deep neural network model, the full-field shear wave is subjected to full-wave inversion operation to obtain the corresponding shear modulus and Young's modulus, and to reconstruct the pixel-level biomechanical properties of the target sample to be tested.

6. A microscopic optical coherence elastic imaging system, characterized in that: include: Multifunctional I / O device, used to generate acquisition trigger signals and output vibration control signals and position control signals at the same time; A real-time image display module, configured to trigger a preset line scan camera through the acquisition trigger signal to acquire a line scan image corresponding to the target sample to be tested, and based on the line scan image, adjust the position of the target sample to be tested in a preset sample stage so that the target sample to be tested is placed at a target test position; A sample excitation module, used to control the vibration of a preset bending actuator or ultrasonic transducer in a target excitation system using the vibration control signal, so as to excite a low-frequency shear wave or ultrasonic wave in a target sample to be tested at the target test position; A reconstruction module is used to scan the target sample to be tested based on the position control signal to obtain the propagation information of the low-frequency shear wave or the ultrasonic wave, and reconstruct the pixel-level biomechanical properties of the target sample to be tested according to the propagation information and a preset near-field full-wave inversion algorithm.

7. The microscopic optical coherence elastic imaging system according to claim 6, characterized in that: The real-time image display module comprises: The line scan camera is used to collect the line scan image corresponding to the target sample to be tested, and perform background noise removal and dispersion correction addition operations on the line scan image to obtain a corresponding interference signal, and perform Fourier transform on the interference signal to generate a corresponding A-line diagram, and calibrate the position of the target sample to be tested according to the interference signal and the A-line diagram so that the target sample to be tested is placed at the target test position of the sample stage.

8. The microscopic optical coherence elastic imaging system according to claim 6, characterized in that: The sample excitation module comprises: A shear wave excitation unit, which is used to generate a corresponding shear wave waveform signal through the vibration control signal when the target excitation system is the shear wave excitation system, and send the shear wave waveform signal to the multifunctional I / O device to generate a corresponding excitation signal, and send the excitation signal to a preset power amplifier to generate a corresponding waveform amplification signal, and drive the bending actuator through the waveform amplification signal to excite the low-frequency shear wave corresponding to the target sample to be tested; An underwater excitation unit is used to set the target test position of the target sample to be tested to a preset sample arm and a focus of the ultrasonic transducer when the target excitation system is the underwater excitation system, and based on the vibration control signal, to make the ultrasonic transducer perform high-frequency mechanical vibration to excite the ultrasonic wave corresponding to the target sample to be tested, or, based on the ultrasonic transducer and a preset acoustic metasurface, to generate a needle-shaped Airy beam to excite the ultrasonic wave corresponding to the target sample to be tested through the needle-shaped Airy beam.

9. The microscopic optical coherence elastic imaging system according to claim 6, characterized in that: The reconstruction module includes: A galvanometer, used for adjusting the scanning direction through the position control signal, so as to determine the X-direction galvanometer position and the Y-direction galvanometer position corresponding to the position control signal according to the scanning waveform, so as to scan the target sample to be tested based on the X-direction galvanometer position and the Y-direction galvanometer position, so as to obtain the propagation information corresponding to the low-frequency shear wave or the ultrasonic wave; The full-wave inversion unit is used to perform phase analysis on the propagation information to detect the corresponding full-field shear wave, and based on the preset reverberation shear wave strategy or SWENet deep neural network model, perform full-wave inversion operation on the full-field shear wave to obtain the corresponding shear modulus and Young's modulus, and reconstruct the pixel-level biomechanical properties of the target sample to be tested.

10. An electronic device, characterized in that: include: A memory, a processor, and a computer program stored in the memory and executable on the processor, wherein the processor executes the program to implement the microscopic optical coherence elasticity imaging method according to any one of claims 1 to 5.

Images