PQQ solid beverage capable of promoting collagen synthesis and preparation process of PQQ solid beverage

Abstract

The invention relates to the field of solid beverages, and discloses a PQQ solid beverage capable of promoting collagen synthesis and a preparation technology of the PQQ solid beverage. 2-4 parts of a grape seed extract; 0.2 to 0.8 part of nicotinamide; 0.2 to 0.8 part of L-cysteine; 0.1 to 0.3 part of sodium hyaluronate; 1 to 2 parts of agaricus blazei murill; 0.05 to 0.1 part of pyrroloquinoline quinone disodium salt; 1 to 2 parts of SOD yeast; and 25-35 parts of an auxiliary material. The nicotinamide is used for improving the skin barrier function and promoting cell metabolism, the L-cysteine is used for providing key amino acid for synthesizing collagen and directly supporting collagen generation, and the nicotinamide and the L-cysteine interact with each other, so that generation of deep collagen of the skin is promoted, the anti-aging effect of the skin is remarkably improved, and the structure and elasticity of the skin are improved; therefore, a quicker and more effective anti-aging effect is provided.

Description

Technical Field

[0001] The invention relates to the field of solid beverages, in particular to a PQQ solid beverage for promoting collagen synthesis and a preparation process thereof. Background Art

[0002] With the improvement of living standards in modern society and people's concern for health, collagen supplementation has gradually become an important topic in the field of skin care. As the main component of skin structure, collagen determines the elasticity and firmness of the skin. As people age, the amount of collagen in the human body decreases year by year, leading to aging phenomena such as sagging and wrinkles on the skin. Therefore, promoting collagen synthesis and delaying the aging process have become one of the key directions in the research and development of skin care products.

[0003] However, products on the market that are used to promote collagen synthesis, such as oral collagen supplements, usually face some obvious limitations. First, although oral collagen supplements can provide collagen raw materials, they are broken down into small molecular amino acids during the digestion process, which are difficult to be directly absorbed and utilized, resulting in low absorption efficiency and slow effects. Usually, long-term use is required to see obvious changes. Summary of the invention

[0004] In view of the deficiencies of the prior art, the present invention provides a PQQ solid beverage and a preparation process for promoting collagen synthesis, which solves the problems of low absorption efficiency, slow effect and long-term use of traditional oral collagen supplements to be effective.

[0005] To achieve the above object, the present invention is implemented by the following technical scheme: A PQQ solid beverage for promoting collagen synthesis, comprising the following components in parts by weight:

[0006] White tomato extract: 2-4 parts;

[0007] Grape seed extract: 2-4 parts;

[0008] Niacinamide: 0.2-0.8 parts;

[0009] L-cysteine: 0.2-0.8 parts;

[0010] Sodium hyaluronate: 0.1-0.3 parts;

[0011] Agaricus blazei: 1-2 servings;

[0012] Pyrroloquinoline quinone disodium: 0.05-0.1 part;

[0013] SOD yeast: 1-2 parts;

[0014] Auxiliary materials: 25 to 35 portions.

[0015] Among them, the combination of these ingredients works together to promote collagen synthesis and improve skin elasticity. White tomato extract and grape seed extract are rich in antioxidant ingredients, especially lycopene and proanthocyanidins, which can remove free radicals in the body and reduce oxidative damage, thereby promoting collagen synthesis and skin health. Niacinamide and L-cysteine ​​are used as excipients to support cell repair and metabolism, and sodium hyaluronate can enhance skin moisturizing and elasticity;

[0016] Lycopene, polyphenols, carotenoids and vitamin C in white tomato extract have powerful antioxidant properties, which can resist free radical damage caused by ultraviolet rays and indirectly promote collagen synthesis. Lycopene in particular helps to enhance the skin's antioxidant capacity and inhibit the aging process.

[0017] The proanthocyanidins, grape polyphenols and flavonoids in grape seed extract also have significant antioxidant effects, promote blood circulation, and improve skin's gloss and elasticity.

[0018] Niacinamide helps improve skin barrier function and promotes skin cell renewal and repair.

[0019] L-cysteine ​​is one of the important amino acids for synthesizing collagen. It is essential for skin health. It can promote the synthesis of collagen in the skin and improve skin structure.

[0020] Sodium hyaluronate can lock moisture in the skin, enhance skin hydration, and help stabilize collagen.

[0021] Agaricus blazei has anti-inflammatory effects and enhances immunity, thereby improving skin conditions.

[0022] Pyrroloquinoline quinone disodium promotes the synthesis of collagen and other skin proteins by regulating redox reactions in cells.

[0023] SOD yeast acts as a source of superoxide dismutase, helping to scavenge free radicals and slow down skin aging.

[0024] Preferably, the white tomato extract comprises lycopene, polyphenolic compounds, carotenoids and vitamin C, wherein the content of lycopene is 0.5 to 1.5 parts.

[0025] Lycopene, as a carotenoid, has a strong antioxidant effect. It can effectively inhibit skin damage caused by ultraviolet rays and reduce the degradation of collagen. Carotenoids can also improve the skin's anti-aging ability by increasing the content of antioxidants in the skin. Vitamin C is not only a well-known antioxidant, but also can promote the synthesis of collagen and enhance the elasticity and radiance of the skin.

[0026] Preferably, the grape seed extract comprises proanthocyanidins, grape polyphenols and flavonoids, wherein the content of proanthocyanidins is 1 to 2 parts.

[0027] Proanthocyanidins in grape seed extract are powerful antioxidants that can effectively reduce the generation of free radicals, inhibit oxidation reactions, prevent collagen degradation, and increase the elasticity of blood vessels, thereby helping to promote collagen synthesis and maintain the structural integrity of the skin. Flavonoids and grape polyphenols have anti-aging, anti-inflammatory and antioxidant effects, promoting skin health and preventing the formation of wrinkles.

[0028] Preferably, the auxiliary materials include maltodextrin, calcium carbonate, silicon dioxide and monk fruit powder.

[0029] Maltodextrin: As a common filler and dissolving agent, maltodextrin plays a role in improving the solubility of solid beverages and helping to evenly disperse the ingredients.

[0030] Calcium carbonate: In addition to being a source of calcium supplement, calcium carbonate can also play a role in regulating pH and stabilizing the formula in beverages.

[0031] Silicon Dioxide: As an anti-caking agent, it helps maintain the fluidity of the formula, avoids caking, and ensures product stability.

[0032] Monk fruit powder: As a natural sweetener, monk fruit powder not only provides natural sweetness to beverages, but also adds flavor and avoids the use of chemical sweeteners.

[0033] Preferably, the ratio of the maltodextrin, calcium carbonate, silicon dioxide and monk fruit powder is 30-40:1-1.5:0.2-0.5:1-2.

[0034] Preferably, the fineness of the maltodextrin and monk fruit powder is 40-80 μm, and the fineness of the calcium carbonate and silicon dioxide is 10-20 μm.

[0035] A preparation process of a PQQ solid beverage for promoting collagen synthesis comprises the following steps:

[0036] Initial mixing: white tomato extract, grape seed extract, niacinamide, L-cysteine, sodium hyaluronate, Agaricus blazei, disodium pyrroloquinoline quinone and SOD yeast are mixed into the main ingredients;

[0037] Post-mixing: Mix the auxiliary materials with the main materials and heat and dry them at the same time;

[0038] Grinding: Grind the main material to refine the main material particles;

[0039] Screening: Remove larger particles through a screen to obtain a solid beverage.

[0040] During the initial mixing stage, the active ingredients are mixed to ensure that they are evenly dispersed and to avoid stratification or precipitation of the ingredients.

[0041] In the post-mixing stage, the auxiliary materials are mixed with the main materials and heated and dried to further enhance the uniformity of the mixing, while removing excess moisture to ensure the dryness and stability of the product.

[0042] Grinding: Grinding can refine the main ingredient particles, increase the particle surface area, and improve the solubility of the beverage, thereby improving the taste and digestion and absorption efficiency of the beverage. Smaller particles can also increase the contact area with water and promote the rapid release of ingredients.

[0043] Sieving: Removes larger particles, helping to ensure the smoothness and stability of the final product, preventing lumps or particles from affecting the drinking experience.

[0044] Preferably, in the initial mixing, a double planetary mixer is used at a rotation speed of 200 to 300 rpm for 15 to 20 minutes;

[0045] In the post-mixing, the mixture is mixed for 20 to 30 minutes at a rotation speed of 100 to 150 rpm and a temperature of 40 to 50°C.

[0046] Preferably, the grinding is performed using a planetary grinder at a rotation speed of 300 to 500 rpm for 5 to 10 min.

[0047] Preferably, in the screening, the mesh size is 40 to 80 meshes.

[0048] The present invention provides a PQQ solid beverage and a preparation process for promoting collagen synthesis. It has the following beneficial effects:

[0049] 1. The present invention improves the skin barrier function and promotes cell metabolism through niacinamide, and directly supports collagen production through L-cysteine ​​as a key amino acid for synthesizing collagen. The two interact with each other to effectively promote the production of collagen in the deep layer of the skin, significantly enhance the anti-aging effect of the skin, improve the skin structure and elasticity, and thus provide a faster and more effective anti-aging effect.

[0050] 2. The present invention provides superoxide dismutase through SOD yeast, combined with PQQ, a super antioxidant that can regulate the redox reaction in cells, can remove superoxide free radicals in the body, reduce oxidative damage, and provide more lasting and milder antioxidant protection. At the same time, by slowing down the accumulation of free radicals, it helps reduce the degradation rate of collagen and delays the aging process of the skin.

[0051] 3. The present invention uses the synergistic combination of SOD yeast, niacinamide and L-cysteine. SOD yeast is used to remove free radicals, while niacinamide improves the skin barrier. L-cysteine ​​is used as a key raw material for synthesizing collagen. Therefore, it can act on the skin from multiple levels at the same time, significantly improving the comprehensive effect of skin health, and significantly improving the elasticity, firmness and hydration of the skin. BRIEF DESCRIPTION OF THE DRAWINGS

[0052] Figure 1 It is a schematic diagram of the method flow of the present invention; DETAILED DESCRIPTION

[0053] The following will be combined with the drawings in the specification of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0054] In order to better understand the present invention, the above contents are described in detail below in conjunction with specific embodiments.

[0055] Please refer to the attached Figure 1 The embodiment of the present invention provides a PQQ solid beverage and a preparation process for promoting collagen synthesis:

[0056] Example 1

[0057] Components:

[0058] White tomato extract (including lycopene, polyphenolic compounds, carotenoids and vitamin C, of ​​which the content of lycopene is 1 part): 3 parts;

[0059] Grape seed extract (including proanthocyanidins, grape polyphenols and flavonoids, of which the content of proanthocyanidins is 1 part): 3 parts;

[0060] Niacinamide: 0.5 parts;

[0061] L-cysteine: 0.6 parts;

[0062] Sodium hyaluronate: 0.22 parts;

[0063] Agaricus blazei: 1.3 servings;

[0064] Pyrroloquinoline quinone disodium: 0.07 parts;

[0065] SOD yeast: 1.4 parts;

[0066] Accessories: 30 parts;

[0067] The ratio of auxiliary materials is: maltodextrin: calcium carbonate: silicon dioxide: monk fruit powder = 36:1.3:0.4:1.3, the fineness of maltodextrin and monk fruit powder is 50 μm, and the fineness of calcium carbonate and silicon dioxide is 10 μm.

[0068] Preparation steps:

[0069] Initial mixing: Use a double planetary mixer to mix the main ingredients (white tomato extract, grape seed extract, niacinamide, L-cysteine, sodium hyaluronate, Agaricus blazei, pyrroloquinoline quinone disodium and SOD yeast) at a speed of 270 rpm for 18 minutes;

[0070] Post-mixing: add auxiliary materials and mix for 27 minutes at a speed of 130 rpm and a temperature of 46°C;

[0071] Grinding: Use a planetary grinder at 400 rpm for 7 min;

[0072] Sieving: The sieve aperture is 80 mesh;

[0073] Example 2

[0074] Components:

[0075] White tomato extract (including lycopene, polyphenolic compounds, carotenoids and vitamin C, of ​​which the content of lycopene is 1 part): 3 parts;

[0076] Grape seed extract (including proanthocyanidins, grape polyphenols and flavonoids, of which the content of proanthocyanidins is 1 part): 3 parts;

[0077] Niacinamide: 0.2 parts;

[0078] L-cysteine: 0.2 parts;

[0079] Sodium hyaluronate: 0.22 parts;

[0080] Agaricus blazei: 1.3 servings;

[0081] Pyrroloquinoline quinone disodium: 0.07 parts;

[0082] SOD yeast: 1 part;

[0083] Accessories: 30 parts;

[0084] The ratio of auxiliary materials is: maltodextrin: calcium carbonate: silicon dioxide: monk fruit powder = 36:1.3:0.4:1.3, the fineness of maltodextrin and monk fruit powder is 50 μm, and the fineness of calcium carbonate and silicon dioxide is 10 μm.

[0085] Preparation steps:

[0086] Initial mixing: Use a double planetary mixer to mix the main ingredients (white tomato extract, grape seed extract, niacinamide, L-cysteine, sodium hyaluronate, Agaricus blazei, pyrroloquinoline quinone disodium and SOD yeast) at a speed of 270 rpm for 18 minutes;

[0087] Post-mixing: add auxiliary materials and mix for 27 minutes at a speed of 130 rpm and a temperature of 46°C;

[0088] Grinding: Use a planetary grinder at 400 rpm for 7 min;

[0089] Screening: The screen aperture is 80 mesh.

[0090] Example 3

[0091] Components:

[0092] White tomato extract (including lycopene, polyphenolic compounds, carotenoids and vitamin C, of ​​which the content of lycopene is 1 part): 3 parts;

[0093] Grape seed extract (including proanthocyanidins, grape polyphenols and flavonoids, of which the content of proanthocyanidins is 1 part): 3 parts;

[0094] Niacinamide: 0.8 parts;

[0095] L-cysteine: 0.8 parts;

[0096] Sodium hyaluronate: 0.22 parts;

[0097] Agaricus blazei: 1.3 servings;

[0098] Pyrroloquinoline quinone disodium: 0.07 parts;

[0099] SOD yeast: 2 parts;

[0100] Accessories: 30 parts;

[0101] The ratio of auxiliary materials is: maltodextrin: calcium carbonate: silicon dioxide: monk fruit powder = 36:1.3:0.4:1.3, the fineness of maltodextrin and monk fruit powder is 50 μm, and the fineness of calcium carbonate and silicon dioxide is 10 μm.

[0102] Preparation steps:

[0103] Initial mixing: Use a double planetary mixer to mix the main ingredients (white tomato extract, grape seed extract, niacinamide, L-cysteine, sodium hyaluronate, Agaricus blazei, pyrroloquinoline quinone disodium and SOD yeast) at a speed of 270 rpm for 18 minutes;

[0104] Post-mixing: add auxiliary materials and mix for 27 minutes at a speed of 130 rpm and a temperature of 46°C;

[0105] Grinding: Use a planetary grinder at 400 rpm for 7 min;

[0106] Screening: The screen aperture is 80 mesh.

[0107] Comparative Example 1

[0108] Based on Example 1, the number of components: no niacinamide, and the rest are the same.

[0109] Comparative Example 2

[0110] Based on Example 2, the number of components is: 0.1 part of niacinamide, and the rest are the same.

[0111] Comparative Example 3

[0112] Based on Example 3, the number of components is: 0.9 part of niacinamide, and the rest are the same.

[0113] Comparative Example 4

[0114] Based on Example 1, the number of components: no L-cysteine, and the rest are the same.

[0115] Comparative Example 5

[0116] Based on Example 2, the number of components is: 0.1 part of L-cysteine, and the rest are the same.

[0117] Comparative Example 6

[0118] Based on Example 3, the number of components is: 0.9 part of L-cysteine, and the rest are the same.

[0119] Comparative Example 7

[0120] Based on Example 1, the number of components: no SOD yeast, and the rest are the same.

[0121] Comparative Example 8

[0122] Based on Example 2, the number of components: 0.9 part of SOD yeast, and the rest are the same.

[0123] Comparative Example 9

[0124] Based on Example 3, the number of components: 3 parts of SOD yeast, and the rest are the same.

[0125] Comparative Example 10

[0126] Based on Example 1, nicotinamide, L-cysteine ​​and SOD yeast were omitted, and the rest were the same.

[0127] Experiment 1

[0128] Purpose

[0129] This experiment aims to explore the synergistic effect of niacinamide and L-cysteine ​​on promoting collagen synthesis and antioxidant capacity. By comparing the examples and comparative samples with different component contents, the amount of collagen produced and the antioxidant capacity in skin cells were measured under the same experimental conditions, and their mechanism of action and optimized combination were analyzed.

[0130] Experimental procedures

[0131] 1. Cell Culture

[0132] Human dermal fibroblasts (HDFs) were seeded in 96-well plates and cultured to 80% confluence to ensure a stable experimental environment. DMEM medium (containing 10% FBS) was used for culture and maintained at a constant temperature (37° C.) and 5% CO2 environment.

[0133] 2. Sample settings

[0134] Nine experimental groups were set up, corresponding to Examples 1-3 and Comparative Examples 1-6, each group had six replicate wells, and cultured for 48 hours.

[0135] 3. Determination of collagen content

[0136] Collection of culture fluid: After 48 hours, centrifuge (1500 rpm, 10 min) and take the supernatant.

[0137] Collagen synthesis levels were measured spectrophotometrically at 560 nm using the hydroxyproline (HYP) assay.

[0138] After the standard curve was established, the HYP content in each group was calculated.

[0139] 4. Antioxidant capacity determination

[0140] The DPPH free radical scavenging experiment was used to detect the antioxidant capacity of the culture medium:

[0141] Add 100 μL of DPPH solution (0.2 mM) to each well.

[0142] After reacting for 30 min, the absorbance was read at 517 nm and the free radical scavenging rate was calculated.

[0143] Data analysis

[0144] SPSS statistical software was used for one-way analysis of variance (ANOVA) to calculate the differences among the groups (significant level P<0.05).

[0145] The data were averaged from 3 experiments and the standard errors were calculated.

[0146] Experimental data

[0147] Table 1: Effects of niacinamide and L-cysteine ​​on collagen synthesis and antioxidant capacity

[0148]

[0149] Experimental Summary

[0150] Analysis of Examples 1-3

[0151] Example 1 (0.5 parts of nicotinamide, 0.6 parts of L-cysteine): The amount of collagen synthesis was 48.6 μg / mL, and the DPPH free radical scavenging rate was 75.3%. This shows that the moderate ratio of nicotinamide and L-cysteine ​​can promote the synthesis of collagen well and has a strong antioxidant effect. The presence of nicotinamide enhances the metabolic activity of cells by increasing the level of NAD+, while L-cysteine ​​effectively scavenges free radicals by increasing the level of glutathione in cells, promoting skin repair and anti-aging.

[0152] Example 2 (0.2 parts of nicotinamide and 0.2 parts of L-cysteine): The amount of collagen synthesis was 39.2 μg / mL, and the DPPH free radical scavenging rate was 68.7%. Although this ratio is relatively low, the synergistic effect of the two still has a significant effect. Compared with Example 1, the amount of collagen synthesis was slightly lower, which may be due to the low concentration of nicotinamide and L-cysteine, resulting in insufficient activation of the metabolic activity and antioxidant capacity of the cells.

[0153] Example 3 (0.8 parts of niacinamide and 0.8 parts of L-cysteine): The amount of collagen synthesis was 52.3 μg / mL, and the DPPH free radical scavenging rate was 80.1%. This group showed the strongest effect, indicating that the high dose of niacinamide and L-cysteine ​​significantly promoted the synthesis of collagen and enhanced the antioxidant capacity. The higher concentrations of niacinamide and L-cysteine ​​provide sufficient energy and repair capacity for skin cells, thereby maximizing the promotion of skin repair and collagen production.

[0154] Analysis of Comparative Examples 1-6

[0155] Comparative Example 1 (no nicotinamide, 0.6 parts of L-cysteine): collagen synthesis was 31.8 μg / mL, and DPPH free radical scavenging rate was 52.4%. After removing nicotinamide, the repair ability and metabolic activity of cells decreased significantly, and the synthesis amount and antioxidant capacity of collagen were both low. The lack of nicotinamide led to insufficient energy supply to cells, affecting the synthesis of collagen and free radical scavenging ability.

[0156] Comparative Example 2 (0.1 part nicotinamide, 0.2 part L-cysteine): The amount of collagen synthesis was 33.1 μg / mL, and the DPPH free radical scavenging rate was 55.6%. Although the lower dose of nicotinamide and L-cysteine ​​had a certain effect, it failed to significantly activate cell repair and antioxidant functions. In particular, the amount of collagen synthesis was significantly lower than that of the example group.

[0157] Comparative Example 3 (0.9 parts of nicotinamide and 0.8 parts of L-cysteine): the amount of collagen synthesis was 27.9 μg / mL, and the DPPH free radical scavenging rate was 48.9%. This set of data performed relatively poorly. Although the dosages of nicotinamide and L-cysteine ​​were high, the imbalance of dosages may have caused the redox balance in the cells to be destroyed, which in turn affected the collagen synthesis and antioxidant capacity.

[0158] Comparative Example 4 (0.5 parts of nicotinamide, no L-cysteine): The amount of collagen synthesis was 29.5 μg / mL, and the DPPH free radical scavenging rate was 50.2%. After removing L-cysteine, although nicotinamide provided certain metabolic support, the lack of the antioxidant function provided by L-cysteine ​​resulted in insufficient free radical scavenging ability of cells, and the collagen synthesis ability was not effectively improved.

[0159] Comparative Example 5 (0.2 parts of nicotinamide and 0.1 parts of L-cysteine): The amount of collagen synthesis was 34.7 μg / mL, and the DPPH free radical scavenging rate was 58.1%. Although this group of ratios performed better than other comparative examples, it was still lower than the example group. Low doses of nicotinamide and L-cysteine ​​may not fully activate the repair and metabolic functions of cells, resulting in relatively low collagen synthesis.

[0160] Comparative Example 6 (0.8 parts of nicotinamide and 0.9 parts of L-cysteine): the amount of collagen synthesis was 28.2 μg / mL, and the DPPH free radical scavenging rate was 47.8%. This group of experiments showed that excessive L-cysteine ​​ratio led to an imbalance in the reduction state in cells, inhibited collagen synthesis, and reduced the free radical scavenging ability. Excessive use of a certain component may lead to cell dysfunction.

[0161] Therefore, nicotinamide, as a precursor of NAD+, can enhance the energy metabolism of cells and promote the repair of skin cells. NAD+ not only provides energy for cells, but also participates in DNA repair and anti-aging processes in cells. In the embodiment, nicotinamide promotes the normal metabolism of cells and enhances the ability to synthesize collagen.

[0162] L-cysteine ​​is a precursor of glutathione (GSH), which can effectively remove free radicals in the body and reduce oxidative damage. The presence of L-cysteine ​​improves the antioxidant capacity of cells and slows down the skin aging process. In Example 3, the high ratio of L-cysteine ​​enhances the free radical scavenging ability, so that collagen synthesis is maximized.

[0163] In summary, the synergistic effect of niacinamide and L-cysteine ​​significantly improves the cell's repair ability and antioxidant function. Niacinamide provides energy and repair signals, while L-cysteine ​​improves antioxidant capacity. The two work together to promote the metabolism of skin cells and the synthesis of collagen, thereby enhancing the structure and elasticity of the skin.

[0164] Therefore, according to the experimental results, the high-dose nicotinamide and L-cysteine ​​ratio of Example 3 showed the best effect. In contrast, in Comparative Examples 1-6, the use of nicotinamide or L-cysteine ​​alone or the imbalance of dosage led to a decrease in collagen synthesis and antioxidant capacity. A reasonable ratio of nicotinamide and L-cysteine ​​can significantly improve the repair ability of skin cells, collagen synthesis and antioxidant capacity through synergistic effects, providing a theoretical basis for the development of anti-aging products.

[0165] Experiment 2

[0166] Purpose

[0167] This experiment aims to explore the role of SOD yeast in promoting collagen synthesis and improving antioxidant capacity. By comparing Examples 1-3 and Comparative Examples 7-9, the effects of different SOD yeast concentrations on skin cell repair and antioxidant capacity were evaluated.

[0168] Experimental procedures

[0169] 1. Cell Culture

[0170] Human dermal fibroblasts (HDFs) were seeded in 96-well plates and cultured to 80% confluence to ensure a stable experimental environment. DMEM medium (containing 10% FBS) was used for culture and maintained at a constant temperature (37° C.) and 5% CO2 environment.

[0171] 2. Sample settings

[0172] Six experimental groups were set up, corresponding to Examples 1-3 and Comparative Examples 7-9, each group had six replicate wells, and cultured for 48 hours.

[0173] 3. Determination of collagen content

[0174] Collection of culture fluid: After 48 hours, centrifuge (1500 rpm, 10 min) and take the supernatant.

[0175] Collagen synthesis levels were measured spectrophotometrically at 560 nm using the hydroxyproline (HYP) assay.

[0176] After the standard curve was established, the HYP content in each group was calculated.

[0177] 4. Antioxidant capacity determination

[0178] The DPPH free radical scavenging experiment was used to detect the antioxidant capacity of the culture medium:

[0179] Add 100 μL of DPPH solution (0.2 mM) to each well.

[0180] After reacting for 30 min, the absorbance was read at 517 nm and the free radical scavenging rate was calculated.

[0181] Data analysis

[0182] SPSS statistical software was used for one-way analysis of variance (ANOVA) to calculate the differences among the groups (significant level P<0.05).

[0183] The data were averaged from 3 experiments and the standard errors were calculated.

[0184] Experimental data

[0185] Table 2: Effects of SOD yeast on collagen synthesis and antioxidant capacity

[0186]

[0187] Experimental Summary

[0188] Effect analysis of SOD yeast

[0189] According to the data in the table, Example 3 (2 portions of SOD yeast) showed the best effect in collagen synthesis (52.3 μg / mL) and free radical scavenging rate (80.1%). This shows that SOD yeast can effectively promote the synthesis of collagen at a moderate dose, while enhancing the cell's ability to scavenge free radicals. The superoxide dismutase (SOD) in SOD yeast can scavenge oxidative free radicals in cells, slow down cell aging, and promote cell repair and regeneration. By improving the antioxidant capacity of cells, SOD yeast not only reduces the damage of free radicals to cells, but also indirectly promotes the production of collagen.

[0190] Example 1 (1.4 parts of SOD yeast) and Example 2 (1 part of SOD yeast) showed good effects, with collagen synthesis amounts of 48.6 μg / mL and 39.2 μg / mL, respectively, and DPPH free radical scavenging rates of 75.3% and 68.7%, respectively. The results of these two groups showed that the addition of SOD yeast had a positive effect on cell repair and collagen synthesis, but compared with Example 3, the SOD yeast with a lower dose was insufficient in effect. The low dose of SOD yeast provided weak antioxidant protection, so it failed to significantly increase the amount of collagen synthesis, and the free radical scavenging rate was relatively low.

[0191] Effect analysis of the comparative group

[0192] Compared with the Example group, Comparative Example 7 (no SOD yeast) and Comparative Example 8 (0.9 parts of SOD yeast) showed a significant decrease in effect. In the group without SOD yeast in Comparative Example 7, the collagen synthesis amount was 29.5 μg / mL and the free radical scavenging rate was 50.2%. This shows that cells lacking SOD yeast cannot effectively scavenge free radicals, resulting in accelerated cell aging and significantly reduced collagen synthesis. In the absence of SOD yeast, the repair and regeneration ability of skin cells is significantly limited.

[0193] In Comparative Example 8 (0.9 parts of SOD yeast), the amount of collagen synthesis was 32.4 μg / mL, and the DPPH free radical scavenging rate was 54.6%. Although the amount of SOD yeast increased, it was still not enough to match the effect of the Example group. This may be because the concentration of SOD yeast has not yet reached the level required for the maximum cell repair effect, so the antioxidant capacity and collagen synthesis have not been significantly improved.

[0194] In Comparative Example 9 (3 portions of SOD yeast), although the amount of SOD yeast was relatively high, the amount of collagen synthesis was 46.2 μg / mL and the free radical scavenging rate was 78.2%. Although close to Example 3, it was still slightly lower. This suggests that excessive SOD yeast may affect other metabolic pathways in the cell, leading to an imbalance in the metabolic balance of the cell, affecting the further improvement of collagen synthesis and antioxidant capacity.

[0195] Mechanism analysis

[0196] The main function of SOD yeast comes from the superoxide dismutase (SOD) it contains, which can efficiently remove superoxide free radicals in cells, slow down the cell aging process, and keep cells healthy. The removal of free radicals not only protects skin cells from damage, but also provides a favorable environment for the synthesis of collagen.

[0197] The synthesis of collagen requires the support of cellular energy, and nicotinamide promotes cellular energy metabolism by providing NAD+, providing the necessary energy basis for collagen synthesis. L-cysteine, as a precursor of glutathione, enhances the antioxidant capacity of cells, while SOD yeast further enhances the repair and anti-aging capabilities of cells by scavenging free radicals. The synergistic effect of the two enhances the repair ability of skin cells and promotes the synthesis of collagen.

[0198] From the experimental data, SOD yeast has a significant promoting effect on collagen synthesis and antioxidant capacity when used in appropriate amounts. Example 3 (2 portions of SOD yeast) shows the best effect, indicating that a moderate dose of SOD yeast can maximize its antioxidant and repair functions. Excessive use (such as comparative example 9) may lead to an imbalance in cell metabolism and affect the final effect. Therefore, in skin care products, the dosage of SOD yeast should be adjusted according to the needs of skin cells to achieve the best anti-aging effect.

[0199] Experiment 3

[0200] Purpose

[0201] This experiment aims to explore the effects of nicotinamide, L-cysteine ​​and SOD yeast on promoting collagen synthesis and improving antioxidant capacity. By comparing Example 1 with Comparative Example 10, the effects of nicotinamide, L-cysteine ​​and SOD yeast on promoting collagen synthesis and improving antioxidant capacity were evaluated.

[0202] Experimental procedures

[0203] 1. Cell Culture

[0204] Human dermal fibroblasts (HDFs) were seeded in 96-well plates and cultured to 80% confluence to ensure a stable experimental environment. DMEM medium (containing 10% FBS) was used for culture and maintained at a constant temperature (37° C.) and 5% CO2 environment.

[0205] 2. Sample settings

[0206] Six experimental groups were set up, corresponding to Example 1 and Comparative Example 10, each group had six replicate wells, and cultured for 48 hours.

[0207] 3. Determination of collagen content

[0208] Collection of culture fluid: After 48 hours, centrifuge (1500 rpm, 10 min) and take the supernatant.

[0209] Collagen synthesis levels were measured spectrophotometrically at 560 nm using the hydroxyproline (HYP) assay.

[0210] After the standard curve was established, the HYP content in each group was calculated.

[0211] 4. Antioxidant capacity determination

[0212] The DPPH free radical scavenging experiment was used to detect the antioxidant capacity of the culture medium:

[0213] Add 100 μL of DPPH solution (0.2 mM) to each well.

[0214] After reacting for 30 min, the absorbance was read at 517 nm and the free radical scavenging rate was calculated.

[0215] Data analysis

[0216] SPSS statistical software was used for one-way analysis of variance (ANOVA) to calculate the differences among the groups (significant level P<0.05).

[0217] The data were averaged from 3 experiments and the standard errors were calculated.

[0218] Experimental data

[0219] Table 3: Effects of niacinamide, L-cysteine ​​and SOD yeast on collagen synthesis and antioxidant capacity

[0220]

[0221] Experimental Summary

[0222] The synergistic effect of SOD yeast, nicotinamide and L-cysteine ​​is very significant in skin repair and anti-aging. The results of Example 1 (collagen synthesis amount is 52.6μg / mL, DPPH free radical scavenging rate is 79.5%) are significantly better than those of Comparative Example 10 (collagen synthesis amount is 35.2μg / mL, free radical scavenging rate is 60.4%). Through comparative analysis, it is found that when nicotinamide, L-cysteine ​​and SOD yeast act together, they can significantly improve the collagen synthesis and antioxidant capacity of cells. This is because nicotinamide promotes energy metabolism in cells by participating in the generation of NAD+, thereby providing power for the synthesis of collagen; L-cysteine ​​exerts an antioxidant effect by enhancing the synthesis of glutathione; SOD yeast slows down the aging process of cells by scavenging free radicals. The synergistic effect of the three enables skin cells to maintain cell activity and antioxidant capacity while repairing.

[0223] From a mechanistic perspective, niacinamide helps collagen synthesis by regulating cell metabolism and providing energy, while the synergistic effect of L-cysteine ​​and SOD yeast reduces oxidative stress in cells and slows down collagen degradation. The collaboration between the two provides an ideal environment for skin cells, helping to maintain skin elasticity and toughness. In particular, in the experimental group, the combination of niacinamide, L-cysteine ​​and SOD yeast showed a strong anti-aging effect. This phenomenon shows that the ingredients in skin care formulas need to work together to maximize their repair and maintenance effects.

[0224] In summary, the synergistic effect between niacinamide, L-cysteine ​​and SOD yeast significantly improves the repair and antioxidant capacity of skin cells, especially in collagen synthesis and free radical scavenging, showing very ideal results. In future skin care products, it is possible to consider a reasonable ratio of these three ingredients to achieve better skin care effects.

[0225] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A PQQ solid beverage for promoting collagen synthesis, characterized in that: Includes the following mass components: White tomato extract: 2-4 parts; Grape seed extract: 2-4 parts; Niacinamide: 0.2-0.8 parts; L-cysteine: 0.2-0.8 parts; Sodium hyaluronate: 0.1-0.3 parts; Agaricus blazei: 1-2 servings; Pyrroloquinoline quinone disodium: 0.05-0.1 part; SOD yeast: 1-2 parts; Auxiliary materials: 25 to 35 portions.

2. A PQQ solid beverage for promoting collagen synthesis according to claim 1, characterized in that: The white tomato extract comprises lycopene, polyphenol compounds, carotenoids and vitamin C, wherein the content of lycopene is 0.5 to 1.5 parts.

3. The PQQ solid beverage for promoting collagen synthesis according to claim 1, characterized in that: The grape seed extract comprises proanthocyanidins, grape polyphenols and flavonoids, wherein the content of proanthocyanidins is 1 to 2 parts.

4. The PQQ solid beverage for promoting collagen synthesis according to claim 1, characterized in that: The auxiliary materials include maltodextrin, calcium carbonate, silicon dioxide and monk fruit powder.

5. The PQQ solid beverage for promoting collagen synthesis according to claim 4, characterized in that: The ratio of the maltodextrin, calcium carbonate, silicon dioxide and monk fruit powder is 30-40:1-1.5:0.2-0.5:1-2.

6. The PQQ solid beverage for promoting collagen synthesis according to claim 4, characterized in that: The fineness of the maltodextrin and monk fruit powder is 40-80 μm, and the fineness of the calcium carbonate and silicon dioxide is 10-20 μm.

7. A process for preparing a PQQ solid beverage for promoting collagen synthesis, based on the PQQ solid beverage for promoting collagen synthesis according to any one of claims 1 to 6, characterized in that: The following steps are involved: Initial mixing: white tomato extract, grape seed extract, niacinamide, L-cysteine, sodium hyaluronate, Agaricus blazei, disodium pyrroloquinoline quinone and SOD yeast are mixed into the main ingredients; Post-mixing: Mix the auxiliary materials with the main materials and heat and dry them at the same time; Grinding: Grind the main material to refine the main material particles; Screening: Remove larger particles through a screen to obtain a solid beverage.

8. The process for preparing a PQQ solid beverage for promoting collagen synthesis according to claim 7, characterized in that: In the initial mixing, a double planetary mixer is used at a rotation speed of 200 to 300 rpm for 15 to 20 minutes; In the post-mixing, the mixture is mixed for 20 to 30 minutes at a rotation speed of 100 to 150 rpm and a temperature of 40 to 50°C.

9. The process for preparing a PQQ solid beverage for promoting collagen synthesis according to claim 7, characterized in that: The grinding is performed using a planetary grinder at a rotation speed of 300 to 500 rpm for 5 to 10 minutes.

10. The process for preparing a PQQ solid beverage for promoting collagen synthesis according to claim 7, characterized in that: In the screening, the mesh size is 40 to 80 meshes.

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