Compatibility relationship analysis system based on effects of auricularia auricula polysaccharide and schisandra chinensis

Abstract

The invention discloses a compatibility relationship analysis system based on the efficacy of auricularia auricula polysaccharide and schisandra chinensis, and relates to the technical field of medicine compatibility relationship analysis, the compatibility relationship analysis system comprises a compatibility data acquisition module, a compatibility data processing module, an efficacy index monitoring module, a compatibility relationship analysis module and a compatibility signal transmission module, acquiring compatibility data by utilizing acquisition equipment; the compatibility data processing module is used for preprocessing the compatibility data and acquiring the efficacy index of interaction of the black fungus data and the schisandra chinensis data; the efficacy index monitoring module utilizes a neural network algorithm to construct a black fungus and schisandra chinensis efficacy index model, the ultrasonic extraction technology, the macroporous resin adsorption technology and the Soxhlet extraction technology in the method are closely combined with the modern information technology, and the efficacy index of interaction of black fungus data and schisandra chinensis data is obtained; and real-time and comprehensive monitoring on the compatibility relation of the auricularia auricula polysaccharide and the schisandra chinensis is achieved.

Description

Technical Field

[0001] The invention relates to the technical field of drug compatibility relationship analysis, and in particular to a compatibility relationship analysis system based on the efficacy of black fungus polysaccharide and schisandra chinensis. Background Art

[0002] At a time when drug development and traditional medicine research are constantly deepening, accurate analysis of the compatibility relationship of drug ingredients is crucial. However, existing technologies have many limitations in analyzing the compatibility relationship of black fungus polysaccharides and schisandra chinensis. On the one hand, it is difficult to comprehensively evaluate the complex interaction mechanism between black fungus polysaccharides and various chemical components in schisandra chinensis. Black fungus polysaccharides have diverse structures, and schisandra chinensis contains a variety of chemical components. There are many ways for them to interact with each other, which makes it difficult for existing technologies to explore in depth, resulting in the inability to fully tap the potential medicinal value of the two. On the other hand, this directly leads to a low utilization rate of schisandra chinensis. In the development of related products, schisandra chinensis cannot be reasonably utilized based on accurate compatibility relationships, resulting in a waste of resources. A compatibility relationship analysis system based on the efficacy of black fungus polysaccharides and schisandra chinensis came into being, which taps into the potential medicinal value of black fungus polysaccharides and schisandra chinensis, promotes the research and development innovation of related drugs and health products, and enables the deep integration of traditional Chinese medicine theory with modern science and technology.

[0003] Although the existing technology has made great progress in the analysis of drug compatibility relationships, there are still some problems that need to be optimized. The existing technology is unable to evaluate the interaction between black fungus polysaccharides and the various chemical components in Schisandra chinensis, resulting in low utilization of Schisandra chinensis and incomplete analysis of the compatibility relationship between black fungus polysaccharides and Schisandra chinensis. Summary of the invention

[0004] The object of the present invention is to provide a compatibility relationship analysis system based on the efficacy of black fungus polysaccharides and Schisandra chinensis to solve the problems raised in the above background technology.

[0005] In order to solve the above technical problems, the technical solution adopted by the present invention is: a compatibility relationship analysis system based on the efficacy of black fungus polysaccharide and Schisandra chinensis, a compatibility data acquisition module, a compatibility data processing module, an efficacy index monitoring module, a compatibility relationship analysis module and a compatibility signal transmission module, wherein each module is communicatively connected;

[0006] The compatibility data acquisition module uses an acquisition device to acquire compatibility data, wherein the compatibility data includes black fungus data, schisandra chinensis data and experimental sample data, providing data support for studying the compatibility relationship between black fungus polysaccharides and schisandra chinensis efficacy;

[0007] The compatibility data processing module pre-processes the compatibility data, obtains the efficacy index of the interaction between the black fungus data and the Schisandra chinensis data, and realizes the monitoring of the efficacy compatibility relationship between the black fungus polysaccharide and the Schisandra chinensis;

[0008] The efficacy index monitoring module uses a neural network algorithm to construct a black fungus and Schisandra chinensis efficacy index model, learns and optimizes the interaction relationship between black fungus and Schisandra chinensis, and solves the problem of incomplete analysis of the compatibility relationship between black fungus polysaccharides and Schisandra chinensis in the prior art;

[0009] The compatibility relationship analysis module evaluates the compatibility relationship between the black fungus data and the Schisandra chinensis data;

[0010] The compatibility signal transmission module sends out a corresponding compatibility signal according to the evaluation result.

[0011] A further improvement of the technical solution of the present invention is that the process of obtaining the compatibility data by the compatibility data acquisition module using the acquisition device includes:

[0012] The collection equipment includes an ultrasonic extractor, a ceramic membrane, a macroporous resin adsorption column, a Soxhlet extractor, a cell collection needle and a mousetrap, the black fungus data are polysaccharides in black fungus, the Schisandra chinensis data are lignans and polysaccharides in Schisandra chinensis, and the experimental sample data are mouse liver cells and healthy mice;

[0013] The ultrasonic extractor emits ultrasonic waves, and the black fungus cells are ruptured under the action of ultrasonic waves, releasing the polysaccharides in the black fungus to obtain the black fungus polysaccharide extract. The black fungus polysaccharide extract is passed through a ceramic membrane, and the polysaccharides in the black fungus are extracted by utilizing the selective permeability of the ceramic membrane.

[0014] A further improvement of the technical solution of the present invention is that the process of obtaining the Schisandra chinensis data by the compatibility data acquisition module includes:

[0015] Using a macroporous resin adsorption column, based on the porous structure and surface properties of the macroporous resin, the lignin in Schisandra chinensis in the Schisandra chinensis lignans extraction solution is adsorbed;

[0016] The Soxhlet extractor uses the reflux and siphon principle of the solvent to extract the polysaccharides from Schisandra chinensis.

[0017] A further improvement of the technical solution of the present invention is that the process of obtaining experimental sample data by the compatibility data acquisition module includes:

[0018] Mouse liver cells were collected through a cell collection needle combined with a liver puncture biopsy. Healthy mice were captured using a mousetrap cage, and the collected mouse liver cells and healthy mice were used as experimental sample data.

[0019] A further improvement of the technical solution of the present invention is that: the process of the compatibility data processing module preprocessing the compatibility data and obtaining the efficacy index of the interaction between the black fungus data and the Schisandra chinensis data includes:

[0020] The compatibility data were cleaned to remove outliers in the compatibility data, and the polysaccharides in black fungus, the lignans in schisandra chinensis, and the polysaccharides in schisandra chinensis were mixed with water in a ratio of 1:1 to obtain black fungus polysaccharide solution, schisandra chinensis lignan solution, and schisandra chinensis polysaccharide solution, respectively; the polysaccharides in black fungus, the lignans in schisandra chinensis, and water were mixed in a ratio of 1:1:1 to prepare an equal proportion mixture of black fungus polysaccharides and schisandra chinensis lignans; the polysaccharides in black fungus, the polysaccharides in schisandra chinensis, and water were mixed in a ratio of 1:1:1 to prepare an equal proportion mixture of black fungus polysaccharides and schisandra chinensis polysaccharides.

[0021] The efficacy index of the interaction between black fungus data and schisandra data includes the efficacy index of the interaction between black fungus polysaccharide and schisandra chinensis lignans on liver cells, and the efficacy index of the interaction between black fungus polysaccharide and schisandra chinensis polysaccharide on immunity.

[0022] A further improvement of the technical solution of the present invention is that the process of obtaining the efficacy index of the interaction between black fungus polysaccharide and schisandra chinensis lignans on liver cells by the compatibility data processing module includes:

[0023] A control group, an experimental group, a black fungus polysaccharide group and a schisandra chinensis lignan group were set up, and the mouse liver cells were evenly divided and placed in the control group, the model group, the experimental group, the black fungus polysaccharide group and the schisandra chinensis lignan group, wherein 2 ml of clean water was added to the control group; 1 ml of liver injury inducer was added to the model group; 1 ml of liver injury inducer and 2 ml of an equal proportion of a mixture of black fungus polysaccharide and schisandra chinensis lignans were added to the experimental group; 1 ml of liver injury inducer and 2 ml of black fungus polysaccharide solution were added to the black fungus polysaccharide group; 1 ml of liver injury inducer and 2 ml of schisandra chinensis lignan solution were added to the schisandra chinensis lignan group, and the liver cells of each group were cultured for 2 hours, and then the MTT method was used to add the MTT solution, and the culture was continued for 1 hour, the supernatant of each group of solutions was removed, DMSO was added, and the absorbance value was determined by using an enzyme marker, and the liver cell viability index of each group was determined according to the absorbance value, and the process of obtaining the efficacy index of the interaction between black fungus polysaccharide and schisandra chinensis lignans on liver protection by calculation was as follows:

[0024]

[0025]

[0026]

[0027]

[0028]

[0029] in, , , and They are the model group, experimental group, black fungus polysaccharide group and schisandra chinensis lignan group on hepatocyte efficacy index. is the efficacy index of the interaction between black fungus polysaccharide and Schisandra chinensis lignans on liver protection, , , , and They were the control group, model group, experimental group, black fungus polysaccharide group and schisandra chinensis lignans group.

[0030] A further improvement of the technical solution of the present invention is that the process of obtaining the efficacy index of the interaction between black fungus polysaccharide and schisandra chinensis polysaccharide on immunity by the compatibility data processing module includes:

[0031] The mice were weighed, and a control group, a black fungus polysaccharide group, a schisandra polysaccharide group and an experimental group were set up, wherein the control group used normal saline to feed the mice, the black fungus polysaccharide group used black fungus polysaccharide solution to feed the mice, the schisandra polysaccharide group used schisandra polysaccharide solution to feed the mice, and the experimental group used an equal proportion of a mixture of black fungus polysaccharide and schisandra polysaccharide to feed the mice, the immune organs of the mice after feeding were collected, the weight of the immune organs was weighed, and the process of calculating the immune organ index of each group using the weight of the immune organs and the weight of the mice was as follows:

[0032]

[0033] Among them, L is the set immune organ index of each group, is the weight of immune organs, is the weight of mice, and the immune organ indexes of the control group, black fungus polysaccharide group, schisandra polysaccharide group and experimental group were obtained respectively;

[0034] The peripheral blood of each group of mice after feeding was collected, and the peripheral blood of each group of mice after feeding was added into a centrifuge tube containing red blood cell lysis solution to lyse the red blood cells in the peripheral blood of each group of mice after feeding. The red blood cell fragments and supernatant after lysis were removed by centrifugation to obtain the white blood cell precipitate of each group of mice. The white blood cell precipitate of each group of mice was washed again by phosphate buffer and centrifugation operation to obtain the white blood cell precipitate of each group of mice. The white blood cell precipitate of each group of mice was added into a flow tube, and CD4 was detected by CD4 fluorescent antibody, CD8 fluorescent antibody and CD19 fluorescent antibody respectively. + T cells, CD8 + T cells and B cells were stained and CD4 + T cells, CD8 + The ratio of T cells and B cells to the total number of lymphocytes;

[0035] The immunity test results are the immune organ index and CD4 + T cells, CD8 + The proportion of T cells and B cells in the total number of lymphocytes, the immune test results data are standardized, and the immune organ index weight and CD4 are set according to the impact of the immune test results data on immunity. + T cells, CD8 + The weight of the proportion of T cells and B cells in the total number of lymphocytes, the process of calculating the efficacy index of the interaction between black fungus polysaccharide and Schisandra polysaccharide on immunity includes:

[0036]

[0037]

[0038]

[0039]

[0040]

[0041] Among them, T is the efficacy index of the interaction between black fungus polysaccharide and Schisandra polysaccharide on immunity; , , and These are the efficacy indexes of the control group, black fungus polysaccharide group, schisandra polysaccharide group and experimental group on immunity; and are immune organ index weight and CD4 + T cells, CD8 + The weight of the proportion of T cells and B cells in the total number of lymphocytes; , , and They are the immune organ indexes of the control group, black fungus polysaccharide group, schisandra chinensis polysaccharide group and experimental group; , , and CD4 + T cells, CD8 + The ratio of T cells to B cells in the total number of lymphocytes.

[0042] A further improvement of the technical solution of the present invention is that the process of constructing the black fungus and Schisandra chinensis efficacy index model in the efficacy index monitoring module includes:

[0043] A neural network model is constructed, and the black fungus data and the schisandra data as well as the efficacy index of the interaction between the black fungus data and the schisandra data are used as data sets, and divided into a training set and a test set according to a ratio of 7:3. MLP is selected as the neural network structure, and the input layer includes two neurons, which receive the black fungus data and the schisandra data, and the hidden layer is configured with an MSE function. The output layer includes two neurons, and outputs the efficacy index of the interaction between the black fungus data and the schisandra data, wherein the input layer input data specifically includes polysaccharides in black fungus, lignans in schisandra, and polysaccharides in schisandra, and the output layer output data specifically includes the efficacy index of the interaction between black fungus polysaccharides and schisandra lignans on hepatocytes, and the efficacy index of the interaction between black fungus polysaccharides and schisandra polysaccharides on immunity;

[0044] Input the training set data into the neural network model, set the learning rate to 0.01, and the number of iterative training to 1000. The training process includes forward propagation and back propagation, wherein the forward propagation is used to calculate the predicted output data, and the back propagation is used to update the weights and biases of the model. Through repeated iterative training, learn the interaction relationship between black fungus polysaccharide and schisandra chinensis lignans on liver cells and the interaction relationship between black fungus polysaccharide and schisandra chinensis polysaccharide on liver cells until the set number of iterative training is reached, and obtain the trained neural network model;

[0045] The test set data was input into the trained neural network model, and the MSE function was used to evaluate the error between the output value of the neural network model and the actual value. The parameters of the neural network model were adjusted according to the evaluation results, the performance of the neural network model was optimized, and the black fungus and Schisandra chinensis efficacy index model was obtained.

[0046] A further improvement of the technical solution of the present invention is that: the process of evaluating the compatibility relationship of the black fungus data and the Schisandra chinensis data efficacy in the compatibility relationship analysis module includes:

[0047] When the efficacy index of the interaction between black fungus polysaccharide and schisandra chinensis lignans on liver cells is lower than 0, it indicates that the compatibility relationship between the interaction between black fungus polysaccharide and schisandra chinensis lignans on liver cells is opposite; when the efficacy index of the interaction between black fungus polysaccharide and schisandra chinensis lignans on liver cells is between 0 and 0.5, it indicates that the compatibility relationship between the interaction between black fungus polysaccharide and schisandra chinensis lignans on liver cells is mutually beneficial; when the efficacy index of the interaction between black fungus polysaccharide and schisandra chinensis lignans on liver cells is greater than 0.5, it indicates that the compatibility relationship between the interaction between black fungus polysaccharide and schisandra chinensis lignans on liver cells is mutually beneficial;

[0048] When the efficacy index of the interaction between black fungus polysaccharides and schisandra polysaccharides on immunity is lower than 0, it indicates that the compatibility relationship between the interaction between black fungus polysaccharides and schisandra polysaccharides on immunity is opposite; when the efficacy index of the interaction between black fungus polysaccharides and schisandra polysaccharides on immunity is between 0 and 0.6, it indicates that the compatibility relationship between the interaction between black fungus polysaccharides and schisandra polysaccharides on immunity is mutually beneficial; when the efficacy index of the interaction between black fungus polysaccharides and schisandra polysaccharides on immunity is greater than 0.6, it indicates that the compatibility relationship between the interaction between black fungus polysaccharides and schisandra polysaccharides on immunity is mutually dependent.

[0049] A further improvement of the technical solution of the present invention is that: the compatibility signal transmission module sends a corresponding compatibility signal according to the evaluation result:

[0050] The green light signal corresponding to the compatibility relationship is set to be mutually dependent, the blue light signal corresponding to the compatibility relationship is set to be mutually beneficial, and the red light signal corresponding to the compatibility relationship is set to be opposite, so as to configure a text display function for the interface of a compatibility relationship analysis system based on the efficacy of black fungus polysaccharide and Schisandra chinensis to display the interacting chemical components;

[0051] When the interaction between black fungus polysaccharide and schisandra chinensis lignans has an opposite compatibility relationship on liver cells, the red light signal is on, and the system interface displays black fungus polysaccharide and schisandra chinensis lignans; when the interaction between black fungus polysaccharide and schisandra chinensis lignans has an opposite compatibility relationship on liver cells, the blue light signal is on, and the system interface displays black fungus polysaccharide and schisandra chinensis lignans; when the interaction between black fungus polysaccharide and schisandra chinensis lignans has an opposite compatibility relationship on liver cells, the green light signal is on, and the system interface displays black fungus polysaccharide and schisandra chinensis lignans;

[0052] When the compatibility relationship between black fungus polysaccharides and schisandra polysaccharides on immunity is opposite, the red light signal is on, and the system interface displays black fungus polysaccharides and schisandra polysaccharides; when the compatibility relationship between black fungus polysaccharides and schisandra polysaccharides on immunity is mutually beneficial, the blue light signal is on, and the system interface displays black fungus polysaccharides and schisandra polysaccharides; when the compatibility relationship between black fungus polysaccharides and schisandra polysaccharides on immunity is mutually dependent, the green light signal is on, and the system interface displays black fungus polysaccharides and schisandra polysaccharides.

[0053] The beneficial effects of the present invention are as follows: compared with the traditional compatibility relationship analysis system of black fungus polysaccharide and schisandra chinensis, the ultrasonic extraction technology, macroporous resin adsorption technology, Soxhlet extraction technology and modern information technology in the method of the present invention are closely combined to accurately capture black fungus polysaccharide, schisandra chinensis lignans and polysaccharide and experimental sample data, obtain the efficacy index of the interaction between black fungus data and schisandra chinensis data, achieve real-time and comprehensive monitoring of the compatibility relationship between black fungus polysaccharide and schisandra chinensis, and learn the efficacy index model of black fungus and schisandra chinensis by constructing the efficacy index model of black fungus and schisandra chinensis. The invention also optimizes the interaction between the components of the two, solves the problem that the prior art cannot evaluate the interaction between the black fungus polysaccharide and the various chemical components in Schisandra chinensis, resulting in low utilization rate of Schisandra chinensis and incomplete analysis of the compatibility relationship between the efficacy of black fungus polysaccharide and Schisandra chinensis, ensures that the system in the invention can refine the dynamic monitoring standards for a compatibility relationship analysis system based on the efficacy of black fungus polysaccharide and Schisandra chinensis within a more precise range, so that the monitored data becomes a more accurate indicator under the same conditions. The development and application of this method significantly enhances the intelligence level in the process of analyzing the compatibility relationship between black fungus polysaccharide and Schisandra chinensis. BRIEF DESCRIPTION OF THE DRAWINGS

[0054] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments recorded in the present invention. For ordinary technicians in this field, other drawings can also be obtained based on these drawings.

[0055] Figure 1 The present invention is a block diagram of a compatibility relationship analysis system based on the efficacy of black fungus polysaccharide and Schisandra chinensis. DETAILED DESCRIPTION

[0056] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0057] like Figure 1 As shown, the present invention provides a compatibility relationship analysis system based on the efficacy of black fungus polysaccharide and Schisandra chinensis, a compatibility data acquisition module, a compatibility data processing module, an efficacy index monitoring module, a compatibility relationship analysis module and a compatibility signal transmission module, wherein each module is communicatively connected;

[0058] The compatibility data acquisition module uses acquisition equipment to obtain compatibility data, where the compatibility data includes black fungus data, Schisandra chinensis data and experimental sample data, providing data support for studying the compatibility relationship between black fungus polysaccharides and Schisandra chinensis efficacy;

[0059] The compatibility data processing module pre-processes the compatibility data, obtains the efficacy index of the interaction between black fungus data and Schisandra chinensis data, and realizes the monitoring of the compatibility relationship between black fungus polysaccharides and Schisandra chinensis;

[0060] The efficacy index monitoring module uses a neural network algorithm to construct a black fungus and Schisandra chinensis efficacy index model, learn and optimize the interaction between black fungus and Schisandra chinensis, and solves the problem of incomplete analysis of the compatibility relationship between black fungus polysaccharides and Schisandra chinensis in the prior art;

[0061] The compatibility relationship analysis module evaluates the compatibility relationship between black fungus data and Schisandra chinensis data;

[0062] The compatibility signal transmission module sends out corresponding compatibility signals according to the evaluation results.

[0063] Preferably, the process of obtaining the compatibility data by the compatibility data acquisition module using the acquisition device includes:

[0064] The collection equipment includes ultrasonic extractors, ceramic membranes, macroporous resin adsorption columns, Soxhlet extractors, cell collection needles and mouse traps. The black fungus data are polysaccharides in black fungus, the Schisandra data are lignans and polysaccharides in Schisandra, and the experimental sample data are mouse liver cells and healthy mice.

[0065] The ultrasonic extractor emits ultrasonic waves, and the black fungus cells are ruptured under the action of ultrasonic waves, releasing the polysaccharides in the black fungus to obtain the black fungus polysaccharide extract. The black fungus polysaccharide extract is passed through a ceramic membrane, and the polysaccharides in the black fungus are extracted by utilizing the selective permeability of the ceramic membrane.

[0066] Preferably, the process of obtaining Schisandrae Chinensis data by the compatibility data collection module includes:

[0067] Using a macroporous resin adsorption column, based on the porous structure and surface properties of the macroporous resin, the lignin in Schisandra chinensis in the Schisandra chinensis lignans extraction solution is adsorbed;

[0068] The Soxhlet extractor uses the reflux and siphon principle of the solvent to extract the polysaccharides from Schisandra chinensis.

[0069] Preferably, the process of obtaining experimental sample data by the compatibility data acquisition module includes:

[0070] Mouse liver cells were collected through a cell collection needle combined with a liver puncture biopsy. Healthy mice were captured using a mousetrap cage, and the collected mouse liver cells and healthy mice were used as experimental sample data.

[0071] Preferably, the process of preprocessing the compatibility data by the compatibility data processing module to obtain the efficacy index of the interaction between the black fungus data and the Schisandra chinensis data includes:

[0072] The compatibility data were cleaned to remove outliers in the compatibility data, and the polysaccharides in black fungus, the lignans in schisandra chinensis, and the polysaccharides in schisandra chinensis were mixed with water in a ratio of 1:1 to obtain black fungus polysaccharide solution, schisandra chinensis lignan solution, and schisandra chinensis polysaccharide solution, respectively; the polysaccharides in black fungus, the lignans in schisandra chinensis, and water were mixed in a ratio of 1:1:1 to prepare an equal proportion mixture of black fungus polysaccharides and schisandra chinensis lignans; the polysaccharides in black fungus, the polysaccharides in schisandra chinensis, and water were mixed in a ratio of 1:1:1 to prepare an equal proportion mixture of black fungus polysaccharides and schisandra chinensis polysaccharides.

[0073] Among them, the efficacy index of the interaction between black fungus data and schisandra data includes the efficacy index of the interaction between black fungus polysaccharide and schisandra chinensis lignans on liver cells, and the efficacy index of the interaction between black fungus polysaccharide and schisandra chinensis polysaccharide on immunity.

[0074] Preferably, the process of obtaining the efficacy index of the interaction between black fungus polysaccharide and schisandra chinensis lignans on liver cells by the compatibility data processing module includes:

[0075] A control group, an experimental group, a black fungus polysaccharide group and a schisandra chinensis lignan group were set up, and the mouse liver cells were evenly divided and placed in the control group, the model group, the experimental group, the black fungus polysaccharide group and the schisandra chinensis lignan group, wherein 2 ml of clean water was added to the control group; 1 ml of liver injury inducer was added to the model group; 1 ml of liver injury inducer and 2 ml of an equal proportion of a mixture of black fungus polysaccharide and schisandra chinensis lignans were added to the experimental group; 1 ml of liver injury inducer and 2 ml of black fungus polysaccharide solution were added to the black fungus polysaccharide group; 1 ml of liver injury inducer and 2 ml of schisandra chinensis lignan solution were added to the schisandra chinensis lignan group, and the liver cells of each group were cultured for 2 hours, and then the MTT method was used to add the MTT solution, and the culture was continued for 1 hour, the supernatant of each group of solutions was removed, DMSO was added, and the absorbance value was determined by using an enzyme marker, and the liver cell viability index of each group was determined according to the absorbance value, and the process of obtaining the efficacy index of the interaction between black fungus polysaccharide and schisandra chinensis lignans on liver protection by calculation was as follows:

[0076]

[0077]

[0078]

[0079]

[0080]

[0081] in, , , and They are the model group, experimental group, black fungus polysaccharide group and schisandra chinensis lignan group on hepatocyte efficacy index. is the efficacy index of the interaction between black fungus polysaccharide and Schisandra chinensis lignans on liver protection, , , , and They were the control group, model group, experimental group, black fungus polysaccharide group and schisandra chinensis lignans group.

[0082] Preferably, the process of obtaining the efficacy index of the interaction between black fungus polysaccharide and schisandra chinensis polysaccharide on immunity by the compatibility data processing module includes:

[0083] The mice were weighed, and a control group, a black fungus polysaccharide group, a schisandra polysaccharide group and an experimental group were set up, wherein the control group used normal saline to feed the mice, the black fungus polysaccharide group used black fungus polysaccharide solution to feed the mice, the schisandra polysaccharide group used schisandra polysaccharide solution to feed the mice, and the experimental group used an equal proportion of a mixture of black fungus polysaccharide and schisandra polysaccharide to feed the mice, the immune organs of the mice after feeding were collected, the weight of the immune organs was weighed, and the process of calculating the immune organ index of each group using the weight of the immune organs and the weight of the mice was as follows:

[0084]

[0085] Among them, L is the set immune organ index of each group, is the weight of immune organs, is the weight of mice, and the immune organ indexes of the control group, black fungus polysaccharide group, schisandra polysaccharide group and experimental group were obtained respectively;

[0086] The peripheral blood of each group of mice after feeding was collected, and the peripheral blood of each group of mice after feeding was added into a centrifuge tube containing red blood cell lysis solution to lyse the red blood cells in the peripheral blood of each group of mice after feeding. The red blood cell fragments and supernatant after lysis were removed by centrifugation to obtain the white blood cell precipitate of each group of mice. The white blood cell precipitate of each group of mice was washed again by phosphate buffer and centrifugation operation to obtain the white blood cell precipitate of each group of mice. The white blood cell precipitate of each group of mice was added into a flow tube, and CD4 was detected by CD4 fluorescent antibody, CD8 fluorescent antibody and CD19 fluorescent antibody respectively. + T cells, CD8 +T cells and B cells were stained and CD4 + T cells, CD8 + The ratio of T cells and B cells to the total number of lymphocytes;

[0087] Among them, the immune test results are the immune organ index and CD4 + T cells, CD8 + The proportion of T cells and B cells in the total number of lymphocytes, the immune test results data are standardized, and the immune organ index weight and CD4 are set according to the impact of the immune test results data on immunity. + T cells, CD8 + The weight of the proportion of T cells and B cells in the total number of lymphocytes, the process of calculating the efficacy index of the interaction between black fungus polysaccharide and Schisandra polysaccharide on immunity includes:

[0088]

[0089]

[0090]

[0091]

[0092]

[0093] Among them, T is the efficacy index of the interaction between black fungus polysaccharide and Schisandra polysaccharide on immunity; , , and These are the efficacy indexes of the control group, black fungus polysaccharide group, schisandra polysaccharide group and experimental group on immunity; and are immune organ index weight and CD4 + T cells, CD8 + The weight of the proportion of T cells and B cells in the total number of lymphocytes; , , and They are the immune organ indexes of the control group, black fungus polysaccharide group, schisandra chinensis polysaccharide group and experimental group; , , and CD4 + T cells, CD8 +The ratio of T cells to B cells in the total number of lymphocytes.

[0094] Preferably, the efficacy index monitoring module, the process of constructing the black fungus and Schisandra chinensis efficacy index model comprises:

[0095] A neural network model is constructed, and the black fungus data and the schisandra data as well as the efficacy index of the interaction between the black fungus data and the schisandra data are used as data sets, and divided into a training set and a test set according to a ratio of 7:3. MLP is selected as the neural network structure, and the input layer includes two neurons, which receive the black fungus data and the schisandra data, and the hidden layer is configured with an MSE function. The output layer includes two neurons, and outputs the efficacy index of the interaction between the black fungus data and the schisandra data, wherein the input layer input data specifically includes polysaccharides in black fungus, lignans in schisandra, and polysaccharides in schisandra, and the output layer output data specifically includes the efficacy index of the interaction between black fungus polysaccharides and schisandra lignans on hepatocytes, and the efficacy index of the interaction between black fungus polysaccharides and schisandra polysaccharides on immunity;

[0096] Input the training set data into the neural network model, set the learning rate to 0.01, and the number of iterative training to 1000. The training process includes forward propagation and back propagation, wherein the forward propagation is used to calculate the predicted output data, and the back propagation is used to update the weights and biases of the model. Through repeated iterative training, learn the interaction relationship between black fungus polysaccharide and schisandra chinensis lignans on liver cells and the interaction relationship between black fungus polysaccharide and schisandra chinensis polysaccharide on liver cells until the set number of iterative training is reached, and obtain the trained neural network model;

[0097] The test set data was input into the trained neural network model, and the MSE function was used to evaluate the error between the output value of the neural network model and the actual value. The parameters of the neural network model were adjusted according to the evaluation results, the performance of the neural network model was optimized, and the black fungus and Schisandra chinensis efficacy index model was obtained.

[0098] Preferably, the process of the compatibility relationship analysis module for evaluating the compatibility relationship between the black fungus data and the Schisandra chinensis data includes:

[0099] When the efficacy index of the interaction between black fungus polysaccharide and schisandra chinensis lignans on liver cells is lower than 0, it indicates that the compatibility relationship between the interaction between black fungus polysaccharide and schisandra chinensis lignans on liver cells is opposite; when the efficacy index of the interaction between black fungus polysaccharide and schisandra chinensis lignans on liver cells is between 0 and 0.5, it indicates that the compatibility relationship between the interaction between black fungus polysaccharide and schisandra chinensis lignans on liver cells is mutually beneficial; when the efficacy index of the interaction between black fungus polysaccharide and schisandra chinensis lignans on liver cells is greater than 0.5, it indicates that the compatibility relationship between the interaction between black fungus polysaccharide and schisandra chinensis lignans on liver cells is mutually beneficial;

[0100] When the efficacy index of the interaction between black fungus polysaccharides and schisandra polysaccharides on immunity is lower than 0, it indicates that the compatibility relationship between the interaction between black fungus polysaccharides and schisandra polysaccharides on immunity is opposite; when the efficacy index of the interaction between black fungus polysaccharides and schisandra polysaccharides on immunity is between 0 and 0.6, it indicates that the compatibility relationship between the interaction between black fungus polysaccharides and schisandra polysaccharides on immunity is mutually beneficial; when the efficacy index of the interaction between black fungus polysaccharides and schisandra polysaccharides on immunity is greater than 0.6, it indicates that the compatibility relationship between the interaction between black fungus polysaccharides and schisandra polysaccharides on immunity is mutually dependent.

[0101] Preferably, the compatibility signal transmission module sends a corresponding compatibility signal according to the evaluation result:

[0102] The green light signal corresponding to the compatibility relationship is set to be mutually dependent, the blue light signal corresponding to the compatibility relationship is set to be mutually beneficial, and the red light signal corresponding to the compatibility relationship is set to be opposite, so as to configure a text display function for the interface of a compatibility relationship analysis system based on the efficacy of black fungus polysaccharide and Schisandra chinensis to display the interacting chemical components;

[0103] When the interaction between black fungus polysaccharide and schisandra chinensis lignans has an opposite compatibility relationship on liver cells, the red light signal is on, and the system interface displays black fungus polysaccharide and schisandra chinensis lignans; when the interaction between black fungus polysaccharide and schisandra chinensis lignans has an opposite compatibility relationship on liver cells, the blue light signal is on, and the system interface displays black fungus polysaccharide and schisandra chinensis lignans; when the interaction between black fungus polysaccharide and schisandra chinensis lignans has an opposite compatibility relationship on liver cells, the green light signal is on, and the system interface displays black fungus polysaccharide and schisandra chinensis lignans;

[0104] When the compatibility relationship between black fungus polysaccharides and schisandra polysaccharides on immunity is opposite, the red light signal is on, and the system interface displays black fungus polysaccharides and schisandra polysaccharides; when the compatibility relationship between black fungus polysaccharides and schisandra polysaccharides on immunity is mutually beneficial, the blue light signal is on, and the system interface displays black fungus polysaccharides and schisandra polysaccharides; when the compatibility relationship between black fungus polysaccharides and schisandra polysaccharides on immunity is mutually dependent, the green light signal is on, and the system interface displays black fungus polysaccharides and schisandra polysaccharides.

[0105] The above is only a specific implementation of the present application, but the protection scope of the present application is not limited thereto. Any person skilled in the art who is familiar with the present technical field can easily think of changes or substitutions within the technical scope disclosed in the present application, which should be included in the protection scope of the present application. Therefore, the protection scope of the present application should be based on the protection scope of the claims.

Claims

1. A compatibility relationship analysis system based on the efficacy of black fungus polysaccharide and Schisandra chinensis, characterized in that: It includes a compatibility data acquisition module, a compatibility data processing module, an efficacy index monitoring module, a compatibility relationship analysis module and a compatibility signal transmission module, wherein each module is connected in communication; The compatibility data acquisition module uses an acquisition device to acquire compatibility data, wherein the compatibility data includes black fungus data, Schisandra chinensis data, and experimental sample data; The compatibility data processing module pre-processes the compatibility data to obtain the efficacy index of the interaction between the black fungus data and the Schisandra chinensis data; The efficacy index monitoring module uses a neural network algorithm to construct an efficacy index model of black fungus and Schisandra chinensis; The compatibility relationship analysis module evaluates the compatibility relationship between the black fungus data and the Schisandra chinensis data; The compatibility signal transmission module sends out a corresponding compatibility signal according to the evaluation result.

2. A compatibility relationship analysis system based on the efficacy of black fungus polysaccharide and Schisandra chinensis according to claim 1, characterized in that: The process of obtaining the compatibility data by the compatibility data acquisition module using the acquisition device includes: The collection equipment includes an ultrasonic extractor, a ceramic membrane, a macroporous resin adsorption column, a Soxhlet extractor, a cell collection needle and a mousetrap, the black fungus data are polysaccharides in black fungus, the Schisandra chinensis data are lignans and polysaccharides in Schisandra chinensis, and the experimental sample data are mouse liver cells and healthy mice; The ultrasonic extractor emits ultrasonic waves, and the black fungus cells are ruptured under the action of ultrasonic waves, releasing the polysaccharides in the black fungus to obtain the black fungus polysaccharide extract. The black fungus polysaccharide extract is passed through a ceramic membrane, and the polysaccharides in the black fungus are extracted by utilizing the selective permeability of the ceramic membrane.

3. A compatibility relationship analysis system based on the efficacy of black fungus polysaccharide and Schisandra chinensis according to claim 2, characterized in that: The process of obtaining Schisandrae Chinensis data by the compatibility data acquisition module includes: Using a macroporous resin adsorption column, based on the porous structure and surface properties of the macroporous resin, the lignin in Schisandra chinensis in the Schisandra chinensis lignans extraction solution is adsorbed; The Soxhlet extractor uses the reflux and siphon principle of the solvent to extract the polysaccharides from Schisandra chinensis.

4. A compatibility relationship analysis system based on the efficacy of black fungus polysaccharide and Schisandra chinensis according to claim 3, characterized in that: The process of obtaining experimental sample data by the compatibility data acquisition module includes: Mouse liver cells were collected through a cell collection needle combined with a liver puncture biopsy. Healthy mice were captured using a mousetrap cage, and the collected mouse liver cells and healthy mice were used as experimental sample data.

5. A compatibility relationship analysis system based on the efficacy of black fungus polysaccharide and Schisandra chinensis according to claim 4, characterized in that: The process of the compatibility data processing module preprocessing the compatibility data and obtaining the efficacy index of the interaction between the black fungus data and the Schisandra chinensis data includes: The compatibility data is cleaned to remove outliers in the compatibility data, and the polysaccharides in black fungus, the lignans in schisandra chinensis, and the polysaccharides in schisandra chinensis are mixed with water in a ratio of 1:1 to obtain black fungus polysaccharide solution, schisandra chinensis lignan solution, and schisandra chinensis polysaccharide solution respectively; the polysaccharides in black fungus, the lignans in schisandra chinensis, and water are mixed in a ratio of 1:1:1 to prepare an equal proportion mixture of black fungus polysaccharides and schisandra chinensis lignans; the polysaccharides in black fungus, the polysaccharides in schisandra chinensis, and water are mixed in a ratio of 1:1:1 to prepare an equal proportion mixture of black fungus polysaccharides and schisandra chinensis polysaccharides. The efficacy index of the interaction between the black fungus data and the schisandra chinensis data includes the efficacy index of the interaction between black fungus polysaccharides and schisandra chinensis lignans on hepatocytes, and the efficacy index of the interaction between black fungus polysaccharides and schisandra chinensis polysaccharides on immunity.

6. A compatibility relationship analysis system based on the efficacy of black fungus polysaccharide and Schisandra chinensis according to claim 5, characterized in that: The process of obtaining the efficacy index of the interaction between black fungus polysaccharide and schisandra chinensis lignans on liver cells by the compatibility data processing module includes: A control group, an experimental group, a black fungus polysaccharide group and a schisandra chinensis lignan group were set up, and the mouse liver cells were evenly divided and placed in the control group, the model group, the experimental group, the black fungus polysaccharide group and the schisandra chinensis lignan group, wherein 2 ml of clean water was added to the control group; 1 ml of liver injury inducer was added to the model group; 1 ml of liver injury inducer and 2 ml of an equal proportion of a mixture of black fungus polysaccharide and schisandra chinensis lignans were added to the experimental group; 1 ml of liver injury inducer and 2 ml of black fungus polysaccharide solution were added to the black fungus polysaccharide group; 1 ml of liver injury inducer and 2 ml of schisandra chinensis lignan solution were added to the schisandra chinensis lignan group, and the liver cells of each group were cultured for 2 hours, and then the MTT method was used to add the MTT solution, and the culture was continued for 1 hour, the supernatant of each group of solutions was removed, DMSO was added, and the absorbance value was determined by using an enzyme marker, and the liver cell viability index of each group was determined according to the absorbance value, and the process of obtaining the efficacy index of the interaction between black fungus polysaccharide and schisandra chinensis lignans on liver protection by calculation was as follows: in, , , and They are the model group, experimental group, black fungus polysaccharide group and schisandra chinensis lignan group on hepatocyte efficacy index. is the efficacy index of the interaction between black fungus polysaccharide and Schisandra chinensis lignans on liver protection, , , , and They were the control group, model group, experimental group, black fungus polysaccharide group and schisandra chinensis lignans group.

7. The compatibility relationship analysis system based on the efficacy of black fungus polysaccharide and Schisandra chinensis according to claim 6, characterized in that: The process of obtaining the efficacy index of the interaction between black fungus polysaccharide and schisandra chinensis polysaccharide on immunity by the compatibility data processing module includes: The mice were weighed, and a control group, a black fungus polysaccharide group, a schisandra polysaccharide group and an experimental group were set up, wherein the control group used normal saline to feed the mice, the black fungus polysaccharide group used black fungus polysaccharide solution to feed the mice, the schisandra polysaccharide group used schisandra polysaccharide solution to feed the mice, and the experimental group used an equal proportion of a mixture of black fungus polysaccharide and schisandra polysaccharide to feed the mice, the immune organs of the mice after feeding were collected, the weight of the immune organs was weighed, and the process of calculating the immune organ index of each group using the weight of the immune organs and the weight of the mice was as follows: Among them, L is the set immune organ index of each group, is the weight of immune organs, is the weight of mice, and the immune organ indexes of the control group, black fungus polysaccharide group, schisandra polysaccharide group and experimental group were obtained respectively; The peripheral blood of each group of mice after feeding was collected, and the peripheral blood of each group of mice after feeding was added into a centrifuge tube containing red blood cell lysis solution to lyse the red blood cells in the peripheral blood of each group of mice after feeding. The red blood cell fragments and supernatant after lysis were removed by centrifugation to obtain the white blood cell precipitate of each group of mice. The white blood cell precipitate of each group of mice was washed again by phosphate buffer and centrifugation operation to obtain the white blood cell precipitate of each group of mice. The white blood cell precipitate of each group of mice was added into a flow tube, and CD4 was detected by CD4 fluorescent antibody, CD8 fluorescent antibody and CD19 fluorescent antibody respectively. + T cells, CD8 + T cells and B cells were stained and CD4 + T cells, CD8 + The ratio of T cells and B cells to the total number of lymphocytes; The immunity test results are the immune organ index and CD4 + T cells, CD8 + The proportion of T cells and B cells in the total number of lymphocytes, the immunity test results data are standardized, and the immune organ index weight and CD4 are set according to the impact of the immunity test results data on immunity. + T cells, CD8 + The weight of the proportion of T cells and B cells in the total number of lymphocytes, the process of calculating the efficacy index of the interaction between black fungus polysaccharide and Schisandra polysaccharide on immunity includes: Among them, T is the efficacy index of the interaction between black fungus polysaccharide and Schisandra polysaccharide on immunity; , , and These are the efficacy indexes of the control group, black fungus polysaccharide group, schisandra polysaccharide group and experimental group on immunity; and are immune organ index weight and CD4 + T cells, CD8 + The weight of the proportion of T cells and B cells in the total number of lymphocytes; , , and They are the immune organ indexes of the control group, black fungus polysaccharide group, schisandra chinensis polysaccharide group and experimental group; , , and CD4 + T cells, CD8 + The ratio of T cells to B cells in the total number of lymphocytes.

8. The compatibility relationship analysis system based on the efficacy of black fungus polysaccharide and Schisandra chinensis according to claim 7, characterized in that: The efficacy index monitoring module, the process of constructing the black fungus and Schisandra chinensis efficacy index model includes: A neural network model is constructed, and the black fungus data and the schisandra data as well as the efficacy index of the interaction between the black fungus data and the schisandra data are used as data sets, which are divided into a training set and a test set in a ratio of 7:

3. MLP is selected as the neural network structure, and the input layer includes two neurons for receiving the black fungus data and the schisandra data, and the hidden layer is configured with the MSE function. The output layer includes two neurons for outputting the efficacy index of the interaction between the black fungus data and the schisandra data, wherein the input layer input data specifically includes polysaccharides in black fungus, lignans in schisandra, and polysaccharides in schisandra, and the output layer output data specifically includes the efficacy index of the interaction between black fungus polysaccharides and schisandra lignans on liver cells, and the efficacy index of the interaction between black fungus polysaccharides and schisandra polysaccharides on immunity; Input the training set data into the neural network model, set the learning rate to 0.01, and the number of iterative training to 1000. The training process includes forward propagation and back propagation, wherein the forward propagation is used to calculate the predicted output data, and the back propagation is used to update the weights and biases of the model. Through repeated iterative training, learn the interaction relationship between black fungus polysaccharide and schisandra chinensis lignans on liver cells and the interaction relationship between black fungus polysaccharide and schisandra chinensis polysaccharide on liver cells until the set number of iterative training is reached, and obtain the trained neural network model; The test set data was input into the trained neural network model, and the MSE function was used to evaluate the error between the output value of the neural network model and the actual value. The parameters of the neural network model were adjusted according to the evaluation results, the performance of the neural network model was optimized, and the black fungus and Schisandra chinensis efficacy index model was obtained.

9. The compatibility relationship analysis system based on the efficacy of black fungus polysaccharide and Schisandra chinensis according to claim 8, characterized in that: The process of the compatibility relationship analysis module for evaluating the compatibility relationship of the efficacy of black fungus data and Schisandra chinensis data includes: When the efficacy index of the interaction between black fungus polysaccharide and schisandra chinensis lignans on liver cells is lower than 0, it indicates that the compatibility relationship between the interaction between black fungus polysaccharide and schisandra chinensis lignans on liver cells is opposite; when the efficacy index of the interaction between black fungus polysaccharide and schisandra chinensis lignans on liver cells is between 0 and 0.5, it indicates that the compatibility relationship between the interaction between black fungus polysaccharide and schisandra chinensis lignans on liver cells is mutually beneficial; when the efficacy index of the interaction between black fungus polysaccharide and schisandra chinensis lignans on liver cells is greater than 0.5, it indicates that the compatibility relationship between the interaction between black fungus polysaccharide and schisandra chinensis lignans on liver cells is mutually beneficial; When the efficacy index of the interaction between black fungus polysaccharides and schisandra polysaccharides on immunity is lower than 0, it indicates that the compatibility relationship between the interaction between black fungus polysaccharides and schisandra polysaccharides on immunity is opposite; when the efficacy index of the interaction between black fungus polysaccharides and schisandra polysaccharides on immunity is between 0 and 0.6, it indicates that the compatibility relationship between the interaction between black fungus polysaccharides and schisandra polysaccharides on immunity is mutually beneficial; when the efficacy index of the interaction between black fungus polysaccharides and schisandra polysaccharides on immunity is greater than 0.6, it indicates that the compatibility relationship between the interaction between black fungus polysaccharides and schisandra polysaccharides on immunity is mutually dependent.

10. The compatibility relationship analysis system based on the efficacy of black fungus polysaccharide and Schisandra chinensis according to claim 9, characterized in that: The compatibility signal transmission module sends a corresponding compatibility signal according to the evaluation result: The green light signal corresponding to the compatibility relationship is set to be mutually dependent, the blue light signal corresponding to the compatibility relationship is set to be mutually beneficial, and the red light signal corresponding to the compatibility relationship is set to be opposite, so as to configure a text display function for the interface of a compatibility relationship analysis system based on the efficacy of black fungus polysaccharide and Schisandra chinensis to display the interacting chemical components; When the interaction between black fungus polysaccharide and schisandra chinensis lignans has an opposite compatibility relationship on liver cells, the red light signal is on, and the system interface displays black fungus polysaccharide and schisandra chinensis lignans; when the interaction between black fungus polysaccharide and schisandra chinensis lignans has an opposite compatibility relationship on liver cells, the blue light signal is on, and the system interface displays black fungus polysaccharide and schisandra chinensis lignans; when the interaction between black fungus polysaccharide and schisandra chinensis lignans has an opposite compatibility relationship on liver cells, the green light signal is on, and the system interface displays black fungus polysaccharide and schisandra chinensis lignans; When the compatibility relationship between black fungus polysaccharides and schisandra polysaccharides on immunity is opposite, the red light signal is on, and the system interface displays black fungus polysaccharides and schisandra polysaccharides; when the compatibility relationship between black fungus polysaccharides and schisandra polysaccharides on immunity is mutually beneficial, the blue light signal is on, and the system interface displays black fungus polysaccharides and schisandra polysaccharides; when the compatibility relationship between black fungus polysaccharides and schisandra polysaccharides on immunity is mutually dependent, the green light signal is on, and the system interface displays black fungus polysaccharides and schisandra polysaccharides.

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