Anti-allergy bacteriostatic toothpaste and preparation method thereof

Abstract

The invention relates to anti-allergy bacteriostatic toothpaste and a preparation method thereof, the anti-allergy bacteriostatic toothpaste is prepared from an anti-allergy composition, a thickening agent, a foaming agent, a friction agent and water, and the anti-allergy composition is prepared from calcium sodium phosphosilicate, hydroxyapatite, potassium chloride and collagen. The toothpaste can accurately act on exposed dentinal tubules, the plugging rate of the dentinal tubules is high, and the calcium sodium phosphosilicate, the hydroxyapatite, the potassium chloride and the collagen have a certain synergistic effect on the anti-allergy effect. The toothpaste is strong in bacteriostatic action, effectively inhibits bacterium breeding, prevents dental plaque and dental calculus from forming, prevents oral diseases such as decayed teeth, gingivitis and periodontitis from the source, and comprehensively protects oral health.

Description

Technical Field

[0001] The invention belongs to the technical field of daily necessities and relates to an anti-allergic and antibacterial toothpaste and a preparation method thereof. Background Art

[0002] In modern society, people are paying more and more attention to oral health, and various oral problems caused by tooth sensitivity and oral bacteria growth seriously affect people's quality of life and oral health.

[0003] Tooth sensitivity is a very common oral problem. The main causes of tooth sensitivity include gum recession, enamel wear, and improper brushing methods. These factors can lead to exposure of dentinal tubules. When the teeth are exposed to external stimuli such as cold, heat, acid, and sweet, the stimulation will be quickly transmitted to the pulp through the dentinal tubules, causing short and sharp pain, which brings many inconveniences to the patient's diet and daily life. Excessive growth of bacteria in the mouth is also a key factor in causing a variety of oral diseases. The mouth is a warm and humid environment, which is very suitable for the growth and reproduction of bacteria. Harmful bacteria such as Streptococcus mutans and Porphyromonas gingivalis multiply in large numbers in the mouth. They will decompose food residues to produce acidic substances, erode teeth, and cause caries; they will also stimulate gingival tissue, leading to diseases such as gingivitis and periodontitis, and in severe cases, even cause loose teeth and loss. In addition, the metabolites produced by oral bacteria can also cause bad breath and affect a person's social and mental health.

[0004] At present, there are many kinds of toothpaste products on the market, but toothpastes for tooth sensitivity and oral bacteria problems still have many deficiencies in terms of efficacy and safety. Some toothpastes that focus on antibacterial effects add a large amount of chemical antibacterial agents, such as triclosan, in order to pursue stronger antibacterial effects. Although these chemical antibacterial agents have significant antibacterial effects, long-term use may damage the oral microecological environment, leading to a decrease in beneficial bacteria in the mouth, drug resistance in harmful bacteria, and even possible absorption into the human body through the oral mucosa, causing potential harm to health.

[0005] With the increasing demand for oral care and the pursuit of natural, safe and effective oral care products, the market urgently needs to develop a toothpaste that can quickly and effectively relieve tooth sensitivity symptoms, safely and durably inhibit the growth of oral bacteria, and is friendly to the oral microecological environment. Summary of the invention

[0006] In view of the deficiencies in the prior art, the object of the present invention is to provide an anti-allergic and antibacterial toothpaste and a preparation method thereof.

[0007] In order to achieve the purpose of the invention, the present invention adopts the following technical solutions:

[0008] In a first aspect, the present invention provides an anti-allergic and bacteriostatic toothpaste. The preparation raw materials of the anti-allergic and bacteriostatic toothpaste include an anti-allergic composition, a thickening agent, a foaming agent, an abrasive, and water. The anti-allergic composition includes calcium sodium phosphosilicate, hydroxyapatite, potassium chloride, and collagen.

[0009] The toothpaste of the present invention can accurately act on the exposed dentinal tubules, has a high blocking rate for the tooth tubules, and calcium sodium phosphosilicate, hydroxyapatite, potassium chloride, and collagen have a certain synergistic effect in terms of anti-allergic effect. The toothpaste has a strong bacteriostatic effect, effectively inhibits the growth of bacteria, prevents the formation of dental plaque and dental calculus, and prevents oral diseases such as dental caries, gingivitis, and periodontitis from the root, comprehensively protecting oral health.

[0010] Preferably, the preparation raw materials of the anti-allergic and bacteriostatic toothpaste include, by mass, 0.5 - 2 parts of the anti-allergic composition, 0.5 - 1.5 parts of the thickening agent, 2 - 4 parts of the foaming agent, 15 - 20 parts of the abrasive, and 28 - 35 parts of water.

[0011] The mass parts of the anti-allergic composition can be selected as 0.5 part, 0.8 part, 1 part, 1.2 parts, 1.4 parts, 1.6 parts, 1.8 parts, 2 parts, etc.; the mass parts of the thickening agent can be selected as 0.5 part, 0.6 part, 0.8 part, 1 part, 1.2 parts, 1.5 parts, etc.; the mass parts of the foaming agent can be selected as 2 parts, 2.2 parts, 2.4 parts, 2.6 parts, 2.8 parts, 3 parts, 3.2 parts, 3.4 parts, 3.6 parts, 3.8 parts, 4 parts, etc.; the mass parts of the abrasive can be selected as 15 parts, 15.5 parts, 16 parts, 16.5 parts, 17 parts, 17.5 parts, 18 parts, 18.5 parts, 19 parts, 19.5 parts, 20 parts, etc.; the mass parts of water can be selected as 28 parts, 28.5 parts, 29 parts, 29.5 parts, 30 parts, 30.5 parts, 31 parts, 31.5 parts, 32 parts, 32.5 parts, 33 parts, 33.5 parts, 34 parts, 34.5 parts, 35 parts, etc. Other specific point values within the above numerical ranges can be selected, and will not be elaborated one by one here.

[0012] Preferably, the anti-allergic composition includes, by mass, 1 - 2 parts of calcium sodium phosphosilicate, 1 - 2 parts of hydroxyapatite, 1 - 2 parts of potassium chloride, and 0.05 - 0.2 part of collagen.

[0013] The mass fraction of calcium sodium phosphosilicate can be selected from 1 part, 1.1 parts, 1.2 parts, 1.3 parts, 1.4 parts, 1.5 parts, 1.6 parts, 1.7 parts, 1.8 parts, 1.9 parts, 2 parts, etc., the mass fraction of hydroxyapatite can be selected from 1 part, 1.1 parts, 1.2 parts, 1.3 parts, 1.4 parts, 1.5 parts, 1.6 parts, 1.7 parts, 1.8 parts, 1.9 parts, 2 parts, etc., the mass fraction of potassium chloride can be selected from 1 part, 1.1 parts, 1.2 parts, 1.3 parts, 1.4 parts, 1.5 parts, 1.6 parts, 1.7 parts, 1.8 parts, 1.9 parts, 2 parts, etc., the mass fraction of collagen can be selected from 0.05 parts, 0.08 parts, 0.1 parts, 0.12 parts, 0.15 parts, 0.18 parts, 0.2 parts, etc., and other specific point values ​​within the above numerical range can be selected, which will not be repeated here one by one.

[0014] Preferably, the thickener comprises cellulose gum and / or xanthan gum.

[0015] Preferably, the abrasive comprises hydrated silica.

[0016] Preferably, the foaming agent includes any one or a combination of at least two of sodium lauryl sulfate, cocamidopropyl betaine, sodium lauryl glucose carboxylate or lauryl glucoside.

[0017] Preferably, the raw materials for preparing the anti-allergic and antibacterial toothpaste further include any one or a combination of at least two of an antibacterial composition, a moisturizer, a colorant, a chelating agent, a preservative, a taste improver, a fragrance or a pH adjuster.

[0018] Preferably, the raw materials for preparing the anti-allergic and antibacterial toothpaste include, by weight, any one of 0.5-1 parts of an antibacterial composition, 30-40 parts of a moisturizer, 0.1-0.5 parts of a colorant, 1-2 parts of a chelating agent, 0.1-0.3 parts of a preservative, 0.1-0.3 parts of a taste improver, 0.8-1.3 parts of a fragrance, or 0.1-0.3 parts of a pH adjuster, or a combination of at least two of them.

[0019] The mass fraction of the antibacterial composition can be selected from 0.5 parts, 0.6 parts, 0.7 parts, 0.8 parts, 0.9 parts, 1 parts, etc., the mass fraction of the moisturizing agent can be selected from 30 parts, 31 parts, 32 parts, 33 parts, 34 parts, 35 parts, 36 parts, 37 parts, 38 parts, 39 parts, 40 parts, etc., the mass fraction of the colorant can be selected from 0.1 parts, 0.2 parts, 0.3 parts, 0.4 parts, 0.5 parts, etc., the mass fraction of the chelating agent can be selected from 1 part, 1.1 parts, 1.2 parts, 1.3 parts, 1.4 parts, 1.5 parts, 1.6 parts, 1.7 parts, 1.8 parts, 1.9 parts, 2 parts, etc., and the mass fraction of the preservative can be selected from 0.1 parts, 0.12 parts, 0.14 parts, 0.16 parts, 0.18 parts, 0.2 parts, 0.2 The mass fractions of the taste improvers can be selected from 0.1 parts, 0.12 parts, 0.14 parts, 0.16 parts, 0.18 parts, 0.2 parts, 0.22 parts, 0.24 parts, 0.26 parts, 0.28 parts, 0.3 parts, etc. The mass fractions of the flavoring agents can be selected from 0.8 parts, 0.9 parts, 1 parts, 1.1 parts, 1.2 parts, 1.3 parts, etc. The mass fractions of the pH adjusters can be selected from 0.1 parts, 0.12 parts, 0.14 parts, 0.16 parts, 0.18 parts, 0.2 parts, 0.22 parts, 0.24 parts, 0.26 parts, 0.28 parts, 0.3 parts, etc. Other specific point values ​​within the above numerical range can be selected, so they will not be described one by one here.

[0020] Preferably, the antibacterial composition comprises dipotassium glycyrrhizinate, tea polyphenols, zinc citrate and eucalyptus essential oil.

[0021] Preferably, the antibacterial composition comprises, by mass, 1-2 parts of dipotassium glycyrrhizinate, 0.5-1 parts of tea polyphenols, 0.5-1 parts of zinc citrate, and 1-2 parts of eucalyptus essential oil.

[0022] The mass fraction of dipotassium glycyrrhizinate can be selected from 1 part, 1.1 parts, 1.2 parts, 1.3 parts, 1.4 parts, 1.5 parts, 1.6 parts, 1.7 parts, 1.8 parts, 1.9 parts, 2 parts, etc., the mass fraction of tea polyphenols can be selected from 0.5 parts, 0.6 parts, 0.7 parts, 0.8 parts, 0.9 parts, 1 parts, etc., the mass fraction of zinc citrate can be selected from 0.5 parts, 0.6 parts, 0.7 parts, 0.8 parts, 0.9 parts, 1 parts, etc., the mass fraction of eucalyptus essential oil can be selected from 1 part, 1.1 parts, 1.2 parts, 1.3 parts, 1.4 parts, 1.5 parts, 1.6 parts, 1.7 parts, 1.8 parts, 1.9 parts, 2 parts, etc., other specific point values ​​within the above numerical range can be selected, and they will not be elaborated here one by one.

[0023] Preferably, the moisturizing agent includes any one of polyethylene glycol-8, sorbitol or xylitol, or a combination of at least two thereof.

[0024] Preferably, the colorant includes titanium dioxide.

[0025] Preferably, the chelating agent includes sodium acid pyrophosphate.

[0026] Preferably, the preservative includes sodium benzoate.

[0027] Preferably, the taste improver includes menthol and / or sucralose.

[0028] Preferably, the fragrance includes essence.

[0029] Preferably, the pH regulator includes sodium hydroxide.

[0030] In a second aspect, the present invention provides a method for preparing the anti-allergic and bacteriostatic toothpaste according to the first aspect, and the preparation method includes: physically mixing the preparation raw materials and defoaming to obtain the toothpaste.

[0031] Compared with the prior art, the present invention has the following beneficial effects:

[0032] The toothpaste of the present invention can accurately act on the exposed dentinal tubules, has a high sealing rate for the tooth tubules, and calcium sodium phosphosilicate, hydroxyapatite, potassium chloride and collagen have a certain synergistic effect in the anti-allergic effect. The toothpaste has a strong bacteriostatic effect, can effectively inhibit the growth of bacteria, prevent the formation of dental plaque and dental calculus, and prevent oral diseases such as dental caries, gingivitis and periodontitis from the root, and comprehensively protects oral health. Detailed Embodiments

[0033] To further elaborate on the technical means and effects adopted by the present invention, the following further illustrates the technical solutions of the present invention in combination with the preferred embodiments of the present invention, but the present invention is not limited to the scope of the embodiments.

[0034] The sources of the active ingredients contained in the products involved in the following examples and comparative examples are as follows (only the active ingredients are shown, and the necessary auxiliary ingredients contained in other commercially available raw materials are not elaborated):

[0035] Calcium sodium phosphosilicate is a product named calcium sodium phosphosilicate purchased from Wuhan Huaxiang Kejie Biotechnology Co., Ltd.;

[0036] Hydroxyapatite is a product named hydroxyapatite purchased from Shanghai Zilin Industry Co., Ltd.;

[0037] Collagen is a product named Yuelixin recombinant type III collagen (ZSS) purchased from Zhejiang Zhuji Juyuan Biotechnology Co., Ltd.;

[0038] Tea polyphenols are a product named tea polyphenols purchased from Shandong Siyang Biotechnology Co., Ltd.;

[0039] Eucalyptus essential oil was purchased from Ji'an Huatianbao Chinese Herbal Medicine Biological Products Factory under the trade name of eucalyptus essential oil;

[0040] Xanthan gum was purchased from Ordos Zhongxuan Biochemical Co., Ltd. under the trade name of xanthan gum RH;

[0041] Cocamidopropyl betaine was purchased from Guangzhou Tianci High-tech Materials Co., Ltd. under the trade name TC-CAB35GL;

[0042] Hydrated silica was purchased from Jiangsu Mingfan Biotechnology Co., Ltd. under the trade name of Silicon Dioxide MⅡ;

[0043] Polyethylene glycol-8 is purchased from Clariant Chemical Technology (Shanghai) Co., Ltd. and Dow Chemical (Shanghai) Co., Ltd. under the trade names of Polyglykol 400C, CARBOWAX SENTRY PEG 400NF; MACROGOL400 Ph.Eur.;

[0044] Trichlorogalactose was purchased from Shandong Kangbao Biochemical Technology Co., Ltd. under the trade name of Sucralose;

[0045] The flavor was purchased from Givaudan Fragrances & Fragrances (Shanghai) Co., Ltd. with the trade name of Lotus LCAAN56200 / 00.

[0046] Example 1

[0047] This embodiment provides an anti-allergic composition, which includes, by mass, 1.5 parts of sodium calcium phosphosilicate, 1.5 parts of hydroxyapatite, 1.5 parts of potassium chloride and 0.1 parts of collagen.

[0048] The preparation method thereof is as follows: physically mixing the preparation raw materials to obtain the product.

[0049] Example 2

[0050] This embodiment provides an anti-allergic composition, which includes, by mass, 1 part of sodium calcium phosphosilicate, 2 parts of hydroxyapatite, 1 part of potassium chloride and 0.2 parts of collagen.

[0051] The preparation method is similar to that of Example 1.

[0052] Example 3

[0053] This embodiment provides an anti-allergic composition, which includes 2 parts of sodium calcium phosphosilicate, 1 part of hydroxyapatite, 2 parts of potassium chloride and 0.05 parts of collagen in parts by mass.

[0054] The preparation method is similar to that of Example 1.

[0055] Example 4

[0056] This embodiment provides an antibacterial composition, which includes, by weight, 1.5 parts of dipotassium glycyrrhizinate, 0.8 parts of tea polyphenols, 0.8 parts of zinc citrate, and 1.5 parts of eucalyptus essential oil.

[0057] The preparation method thereof is as follows: physically mixing the preparation raw materials to obtain the product.

[0058] Example 5

[0059] This embodiment provides an antibacterial composition, which includes, by mass, 1 part of dipotassium glycyrrhizinate, 0.5 parts of tea polyphenols, 1 part of zinc citrate, and 2 parts of eucalyptus essential oil.

[0060] The preparation method is similar to that of Example 4.

[0061] Example 6

[0062] This embodiment provides an antibacterial composition, which includes, by mass, 2 parts of dipotassium glycyrrhizinate, 1 part of tea polyphenols, 0.5 parts of zinc citrate and 1 part of eucalyptus essential oil.

[0063] The preparation method is similar to that of Example 4.

[0064] Example 7

[0065] This embodiment provides an antibacterial composition, which differs from Embodiment 4 only in that it does not contain dipotassium glycyrrhizinate, and its reduced mass is proportionally distributed to the masses of tea polyphenols, zinc citrate and eucalyptus essential oil.

[0066] The preparation method is similar to that of Example 4.

[0067] Example 8

[0068] This embodiment provides an antibacterial composition, which differs from Embodiment 4 only in that it does not contain tea polyphenols, and the reduced mass thereof is proportionally distributed to the masses of dipotassium glycyrrhizinate, zinc citrate and eucalyptus essential oil.

[0069] The preparation method is similar to that of Example 4.

[0070] Example 9

[0071] This embodiment provides an antibacterial composition, which differs from Embodiment 4 only in that it does not contain zinc citrate, and the reduced mass of zinc citrate is proportionally distributed to the masses of dipotassium glycyrrhizinate, tea polyphenols and eucalyptus essential oil.

[0072] The preparation method is similar to that of Example 4.

[0073] Example 10

[0074] This embodiment provides an antibacterial composition, which differs from Embodiment 4 only in that it does not contain eucalyptus essential oil, and its reduced mass is proportionally distributed to the masses of dipotassium glycyrrhizinate, zinc citrate and tea polyphenols.

[0075] The preparation method is similar to that of Example 4.

[0076] Comparative Example 1

[0077] This comparative example provides an anti-allergic composition, which differs from Example 1 only in that it does not contain sodium calcium phosphosilicate, and its reduced mass is proportionally distributed to the masses of hydroxyapatite, potassium chloride and collagen.

[0078] The preparation method is similar to that of Example 1.

[0079] Comparative Example 2

[0080] This comparative example provides an anti-allergic composition, which differs from Example 1 only in that it does not contain hydroxyapatite, and its reduced mass is proportionally distributed to the masses of sodium calcium phosphosilicate, potassium chloride and collagen.

[0081] The preparation method is similar to that of Example 1.

[0082] Comparative Example 3

[0083] This comparative example provides an anti-allergic composition, which differs from Example 1 only in that it does not contain potassium chloride, and its reduced mass is proportionally distributed to the masses of sodium calcium phosphosilicate, hydroxyapatite and collagen.

[0084] The preparation method is similar to that of Example 1.

[0085] Comparative Example 4

[0086] This comparative example provides an anti-allergic composition, which differs from Example 1 only in that it does not contain collagen, and its reduced mass is proportionally distributed to the masses of sodium calcium phosphosilicate, hydroxyapatite and potassium chloride.

[0087] The preparation method is similar to that of Example 1.

[0088] Application Example 1

[0089] This application example provides an anti-sensitivity and antibacterial toothpaste. The raw materials for preparing the toothpaste include, by mass, 1 part of the anti-sensitivity composition of Example 1, 0.8 part of the antibacterial composition of Example 4, 0.5 part of cellulose gum, 0.5 part of xanthan gum, 2 parts of sodium lauryl sulfate, 1 part of cocamidopropyl betaine, 18 parts of hydrated silica, 32 parts of sorbitol, 1 part of polyethylene glycol-8, 1 part of xylitol, 0.3 part of titanium dioxide, 1.5 parts of disodium dihydrogen pyrophosphate, 0.2 part of sodium benzoate, 0.1 part of menthol, 0.1 part of trichlorogalactose, 1 part of flavor, 0.2 part of sodium hydroxide, and 32 parts of water.

[0090] The preparation method comprises the following steps: physically mixing the raw materials and defoaming to obtain the product.

[0091] Application Example 2

[0092] This application example provides an anti-sensitivity and antibacterial toothpaste. The raw materials for preparing the toothpaste include, by mass, 2 parts of the anti-sensitivity composition of Example 2, 0.5 parts of the antibacterial composition of Example 5, 0.25 parts of cellulose gum, 0.25 parts of xanthan gum, 1.5 parts of sodium lauryl sulfate, 0.5 parts of cocamidopropyl betaine, 15 parts of hydrated silica, 28 parts of sorbitol, 1 part of polyethylene glycol-8, 2 parts of xylitol, 0.1 parts of titanium dioxide, 1 part of disodium dihydrogen pyrophosphate, 0.1 parts of sodium benzoate, 0.05 parts of menthol, 0.05 parts of trichlorogalactose, 0.8 parts of flavor, 0.3 parts of sodium hydroxide, and 28 parts of water.

[0093] The preparation method refers to Application Example 1.

[0094] Application Example 3

[0095] This application example provides an anti-sensitivity and antibacterial toothpaste. The raw materials for preparing the toothpaste include, by mass, 0.5 parts of the anti-sensitivity composition of Example 3, 1 part of the antibacterial composition of Example 6, 0.7 parts of cellulose gum, 0.7 parts of xanthan gum, 3 parts of sodium lauryl sulfate, 1 part of cocamidopropyl betaine, 20 parts of hydrated silica, 38 parts of sorbitol, 1 part of polyethylene glycol-8, 1 part of xylitol, 0.5 parts of titanium dioxide, 2 parts of disodium dihydrogen pyrophosphate, 0.3 parts of sodium benzoate, 0.15 parts of menthol, 0.15 parts of trichlorogalactose, 1.3 parts of flavor, 0.1 parts of sodium hydroxide, and 35 parts of water.

[0096] The preparation method refers to Application Example 1.

[0097] Application Example 4-7

[0098] This application example provides 4 kinds of anti-sensitivity and antibacterial toothpastes, which differ from application example 1 only in that the antibacterial composition of example 4 is replaced by the antibacterial compositions of examples 7-10 in equal amounts, and other ingredients and contents remain unchanged.

[0099] The preparation method refers to Application Example 1.

[0100] Comparative Application Examples 1-4

[0101] This comparative application example provides 4 kinds of anti-allergic and antibacterial toothpastes, which differ from Application Example 1 only in that the anti-allergic composition of Example 1 is replaced by the anti-allergic compositions of Comparative Examples 1-4 in equal amounts, and other ingredients and contents remain unchanged.

[0102] The preparation method refers to Application Example 1.

[0103] Test Example 1

[0104] Allergy test

[0105] Dentin hypersensitivity can be treated by applying blocking materials to the dentin surface to seal the exposed dentinal tubules. In this experiment, the contamination layer of bovine dentin slices was removed by acid etching and the opening of dentinal tubules was simulated to establish a repeatable dentin hypersensitivity research model, so as to objectively and accurately evaluate the effect of the test substance in blocking dentinal tubules.

[0106] 3.1 Preparation of dentin samples: Freshly extracted bovine incisors were cut and polished to make dentin blocks of approximately 5 mm × 5 mm × 2 mm in size. The blocks were then corroded with 40% orthophosphoric acid for 20 minutes, rinsed, and then corroded with 5% sodium hypochlorite for 5 minutes. Finally, they were ultrasonically cleaned with deionized water for 20 minutes and observed under a polarized light microscope to ensure that the dental tubules were fully exposed.

[0107] 3.2 Grouping: The same dentin sample was cut into two halves using a low-speed cutter, one half was used as the treated area of ​​the sample group, and the other half was used as the untreated area of ​​the control group.

[0108] 3.3 Preparation of artificial saliva: Dissolve 0.380 g / L sodium chloride, 0.213 g / L calcium chloride dihydrate, 0.738 g / L potassium dihydrogen phosphate, 1.114 g / L potassium chloride, and 2.200 g / L gastric mucin in deionized water, adjust the pH to 7.0 with 1 mol / L sodium hydroxide, add water to make up, and store in a 4°C refrigerator for later use.

[0109] 3.4 Toothpaste slurry preparation: Prepare a uniformly mixed toothpaste slurry according to the mass ratio of 1:1.6 (toothpaste: water).

[0110] 3.5 Treat the dentin samples with toothpaste slurry. Brush the teeth with a toothbrush for 3 minutes, rinse thoroughly with deionized water, and soak in 37°C artificial saliva for 55 minutes. Repeat the brushing for 3 minutes, rinse thoroughly with deionized water, and soak in 37°C artificial saliva overnight. Repeat this step for a total of 7 times. The mass of toothpaste slurry used for each brushing is equal, and 6 replicates are set for each sample.

[0111] 3.6 The dentin samples after the cycle treatment were dried in a constant temperature drying oven at 25°C and then observed using a scanning electron microscope to count the exposed dental tubules C.

[0112] 3.7 Calculation of tubule occlusion rate R (%) in each group

[0113]

[0114] Table 1

[0115] Group Blockage rate (%) Application Example 1 50.1 Application Example 2 47.8 Application Example 3 48.9 Comparative application example 1 35.0 Comparative Application Example 2 33.6 Comparative Application Example 3 36.3 Comparative Application Example 4 36.7

[0116] Test Example 2

[0117] Plaque test

[0118] 1. Test principle: Streptococcus mutans (S. mutans) is the main bacteria in dental plaque that causes dental diseases. Taking mutans streptococcus as the research object, a toothbrush with toothpaste squeezed on it was used to brush the bovine tooth module with mutans streptococcus 50 times, and the number of residual colonies on the bovine tooth module was recovered. The removal rate of dental plaque by the toothpaste was calculated by comparing the number of colonies recovered from the bovine tooth module that was not brushed with a toothbrush.

[0119] 2. Test method:

[0120] Take mutans Streptococcus CICC10387 and inoculate it on TSA agar plate, and culture it anaerobically at 36℃±1℃ for 60h. Use an inoculation loop to take a typical colony of the first generation culture, streak it on TSA agar plate, and culture it anaerobically at 36℃±1℃ for 60h to obtain the second generation culture. Pick and separate the fresh colonies of the third generation, mix them with PBS, and adjust the bacterial concentration to 10 8 CFU / mL, and prepare bacterial suspension for later use.

[0121] Place the disinfected bovine tooth module in a sterile bottle, add 10 mL of bacterial suspension and 10 mL of BHI broth to each bottle and culture anaerobically at 36°C ± 1°C for 24 h.

[0122] The bovine teeth were evenly divided into 8 groups, with 6 teeth in each group. For the sample group, an unused toothbrush was used, and about 1 g of toothpaste was squeezed on the toothbrush. The bovine teeth were fixed and brushed with the toothbrush 50 times back and forth for 7 consecutive days. The toothpaste foam on the surface of the bovine teeth was rinsed off with sterile water, and then the bovine teeth were placed in a sterile homogenization bag and eluted with 20 mL of SCDLP. After anaerobic culture at 36℃±1℃ for 60 hours, the teeth were counted and the average value was recorded as ACFU.

[0123] Six unbrushed bovine teeth were placed in a sterile homogenization bag, each containing 20 mL of SCDLP for elution, and after anaerobic culture at 36°C ± 1°C for 60 h, the teeth were counted and the average value was recorded as B CFU.

[0124] Colony counts were counted in the range of 30 to 300. For each replicate, the number of colonies was calculated by multiplying the number read on the plate by the dilution factor.

[0125] Clearance (%) = (BA) / B × 100%

[0126] Table 2

[0127]

[0128]

[0129] Test Example 3

[0130] Fresh breath effect test

[0131] Test principle: The main components of bad breath gas are volatile sulfides, including methyl mercaptan, hydrogen sulfide and dimethyl sulfide, which are produced by various reasons, about 90% of which come from the mouth. This experiment simulates the use of oral care products in vitro, cultivates the production of volatile sulfides in the oral saliva secretions of volunteers, and measures methyl mercaptan to evaluate the breath freshening effect.

[0132] Test method:

[0133] (1) Cultivating hydroxyapatite sheets overnight to form acquired biofilms;

[0134] (2) Control group: soaked in deionized water for 2 min, taken out and placed in a headspace bottle;

[0135] Sample group: soaked in toothpaste slurry for 2 minutes, taken out and placed in a headspace bottle;

[0136] Preparation method of toothpaste slurry: Mix the sample with water in a mass ratio of 1:3;

[0137] (3) Mix saliva with thioglycollate medium, and mix the hydroxyapatite sheets of the control group and the experimental group with 3 mL of saliva medium;

[0138] (4) Incubate overnight for 12 h and detect the methyl mercaptan peak area.

[0139] Table 3

[0140] Group lg peak area Application Example 1 5.24 Application Example 2 5.85 Application Example 3 5.60 Application Example 4 6.86 Application Example 5 6.71 Application Example 6 6.53 Application Example 7 6.91 Comparative application example 1 7.12 Comparative Application Example 2 7.37 Comparative Application Example 3 6.98 Comparative Application Example 4 7.51 Control group 8.68

[0141] The applicant declares that the present invention illustrates an anti-allergic and antibacterial toothpaste and its preparation method through the above-mentioned embodiments, but the present invention is not limited to the above-mentioned embodiments, that is, it does not mean that the present invention must rely on the above-mentioned embodiments to be implemented. Those skilled in the art should understand that any improvement of the present invention, equivalent replacement of various raw materials of the product of the present invention, addition of auxiliary components, selection of specific methods, etc., all fall within the protection scope and disclosure scope of the present invention.

[0142] The preferred embodiments of the present invention are described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the technical concept of the present invention, a variety of simple modifications can be made to the technical solution of the present invention, and these simple modifications all belong to the protection scope of the present invention.

[0143] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations.

Claims

1. An anti-allergic and antibacterial toothpaste, characterized in that: The raw materials for preparing the anti-allergic and antibacterial toothpaste include an anti-allergic composition, a thickener, a foaming agent, an abrasive and water. The anti-allergic composition includes sodium calcium phosphosilicate, hydroxyapatite, potassium chloride and collagen.

2. The anti-allergic and antibacterial toothpaste according to claim 1, characterized in that: The raw materials for preparing the anti-allergic and antibacterial toothpaste include, by weight, 0.5-2 parts of an anti-allergic composition, 0.5-1.5 parts of a thickener, 2-4 parts of a foaming agent, 15-20 parts of an abrasive, and 28-35 parts of water.

3. The anti-allergic and antibacterial toothpaste according to claim 1 or 2, characterized in that: The anti-allergic composition comprises, by weight, 1-2 parts of sodium calcium phosphosilicate, 1-2 parts of hydroxyapatite, 1-2 parts of potassium chloride and 0.05-0.2 parts of collagen.

4. The anti-allergic and antibacterial toothpaste according to any one of claims 1 to 3, characterized in that: The thickener includes cellulose gum and / or xanthan gum; Preferably, the abrasive comprises hydrated silica; Preferably, the foaming agent includes any one or a combination of at least two of sodium lauryl sulfate, cocamidopropyl betaine, sodium lauryl glucose carboxylate or lauryl glucoside.

5. The anti-allergic and antibacterial toothpaste according to any one of claims 1 to 4, characterized in that: The raw materials for preparing the anti-allergic and antibacterial toothpaste also include any one or a combination of at least two of an antibacterial composition, a moisturizer, a colorant, a chelating agent, a preservative, a taste improver, a fragrance or a pH adjuster.

6. The anti-allergic and antibacterial toothpaste according to claim 5, characterized in that: The raw materials for preparing the anti-allergic and antibacterial toothpaste include, by weight, any one of 0.5-1 parts of an antibacterial composition, 30-40 parts of a moisturizer, 0.1-0.5 parts of a colorant, 1-2 parts of a chelating agent, 0.1-0.3 parts of a preservative, 0.1-0.3 parts of a taste improver, 0.8-1.3 parts of a fragrance, or 0.1-0.3 parts of a pH adjuster, or a combination of at least two of them.

7. The anti-allergic and antibacterial toothpaste according to claim 5 or 6, characterized in that: The antibacterial composition comprises dipotassium glycyrrhizinate, tea polyphenols, zinc citrate and eucalyptus essential oil; Preferably, the antibacterial composition comprises, by mass, 1-2 parts of dipotassium glycyrrhizinate, 0.5-1 parts of tea polyphenols, 0.5-1 parts of zinc citrate and 1-2 parts of eucalyptus essential oil.

8. The anti-allergic and antibacterial toothpaste according to any one of claims 5 to 7, characterized in that: The moisturizer includes any one of polyethylene glycol-8, sorbitol or xylitol, or a combination of at least two thereof; Preferably, the colorant comprises titanium dioxide; Preferably, the chelating agent comprises disodium dihydrogen pyrophosphate; Preferably, the preservative comprises sodium benzoate.

9. The anti-allergic and antibacterial toothpaste according to any one of claims 5 to 8, characterized in that: The taste improver includes menthol and / or galactose; Preferably, the fragrance comprises essence; Preferably, the pH adjuster comprises sodium hydroxide.

10. The method for preparing the anti-allergic and antibacterial toothpaste according to any one of claims 1 to 9, characterized in that: The preparation method comprises: physically mixing the preparation raw materials and defoaming to obtain the product.

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