Quick-acting red fading and relieving composition, application and cosmetic

Abstract

The invention belongs to the field of daily chemicals, and discloses a quick-acting red-fading soothing composition which comprises the following components in parts by weight: 5-15 parts of a Bordeaux leaf extract; 1 to 5 parts of carboxymethyl chitosan; and 20 to 30 parts of glycosylglycerol. According to the composition, the Bordeaux leaf extract is used as a main active component, carboxymethyl chitosan and glycosylglycerol are used as moisturizing and permeation promoting active components, and the carboxymethyl chitosan has an additional soothing function, so that the soothing and red fading effects are optimized. Meanwhile, the invention further discloses application of the composition and cosmetics.

Description

Technical Field

[0001] The invention relates to the field of daily chemicals, and in particular to a fast-acting redness-removing and soothing composition, use and cosmetics. Background Art

[0002] In the field of cosmetics, soothing functional ingredients are an indispensable ingredient in most cosmetic application formulas. They play a vital role in reducing the irritation of whitening ingredients and their applicability to sensitive skin.

[0003] For those skilled in the art, redness reduction can be achieved through the following five pathways: inhibiting inflammatory factors, enhancing antioxidant defenses, repairing the skin lipid barrier and skin barrier, activating the self-repair ability of cells, and calming the skin;

[0004] From the perspective of cell self-repair, an effective way is to use different plant extracts, as shown in the following literature:

[0005] The patent application with publication number CN118236412A, the subject of which is the preparation method, skin care products and uses of Bordeaux leaf extract, discloses a preparation method of Bordeaux leaf extract, and concludes through light protection test that Bordeaux leaf extract has better anti-photoaging, anti-aging and anti-inflammatory effects.

[0006] The patent application with publication number CN114404340A is about a plant anti-acne composition and its application. It clearly states that Bordeaux leaf extract can activate the skin's own defense factor - β-defensin without inducing inflammation, reduce skin redness and restore the skin barrier.

[0007] The patent application with publication number CN116077375A is about a multi-chitosan soothing composition and its application, which records acetyl chitosan, carboxymethyl chitosan, hydroxypropyl chitosan and Sophora japonica bud extract as soothing active ingredients, achieving excellent moisturizing, redness-removing, whitening and repairing effects.

[0008] Glyceryl glucoside generally does not have a soothing effect, but it is widely used in cosmetics with soothing effects. Its main functions are moisturizing and promoting penetration. For example, publication number CN119055568A, a patent application for a soothing composition and its application, uses glycoside compounds such as glyceryl glucoside to reduce surface tension, making it easier for other ingredients to pass through the skin surface, thereby accelerating penetration.

[0009] In the prior art, no one has evaluated the soothing effects of Bordeaux leaf extract, carboxymethyl deacetylated chitosan, and glyceryl glucoside.

[0010] The technical problem solved in this case is how to develop a composition with excellent soothing and redness-removing effects. Summary of the invention

[0011] The object of the present invention is to provide a fast-acting redness-removing and soothing composition, which uses Bordeaux leaf extract as the main active ingredient, carboxymethyl deacetylated chitosan and glycerol glucoside as moisturizing and penetration-promoting active ingredients, and is supplemented with the additional soothing function of carboxymethyl deacetylated chitosan to achieve the optimization of soothing and redness-removing effects.

[0012] At the same time, the invention also discloses a use of the composition and cosmetics.

[0013] To achieve the above objectives, this application discloses:

[0014] A fast-acting redness-removing and soothing composition, comprising the following components in parts by weight:

[0015] 5-15 parts of Bordeaux leaf extract;

[0016] 1-5 parts of carboxymethyl chitosan;

[0017] Glyceryl glucoside 20-30 parts.

[0018] Bordeaux leaf extract is a relatively new raw material on the market. It has soothing properties and oil-control characteristics, so it is mostly used in acne-removing and oil-control products on the market.

[0019] As a relatively excellent soothing active ingredient, improving its soothing and redness-removing effects is the core of this project;

[0020] Through extensive raw material screening, we found that carboxymethyl deacetylated chitosan and glyceryl glucoside can effectively enhance the soothing and redness-removing effect of Bordeaux leaf extract.

[0021] Carboxymethyl chitosan has strong moisturizing and repairing capabilities based on its film-forming properties. Carboxymethyl chitosan is also mentioned to have anti-allergic and antibacterial effects, and can quickly and efficiently relieve itching, making it have a certain soothing effect on the skin. In terms of soothing properties, glyceryl glucoside can reduce the discomfort caused by external stimuli, and is particularly suitable for sensitive skin. It not only provides long-lasting moisturizing, but also reduces redness, swelling and irritation, thereby enhancing the skin barrier function and improving the skin's resistance.

[0022] We believe that its mechanism of soothing and fading redness is:

[0023] The above three substances all have certain soothing effects. When used in combination, they can achieve combined soothing effects from multiple perspectives, including free radical scavenging, anti-oxidation, anti-allergy, anti-bacteria, and skin barrier repair.

[0024] In addition, the main active ingredients of Bordeaux leaf extract are apophenic alkaloids, volatile oils, and flavonol glycosides. Volatile oils are the main soothing ingredients in Bordeaux leaf extracts. Flavonol glycosides can resist oxidation and improve the ability of cells to scavenge free radicals. In an aqueous system, volatile oils are difficult to act quickly on the skin. Flavonol glycosides are compounds formed by flavonols and sugars. Compared with glycerol glucoside, their cell permeability is relatively weak, which is also an obstacle that makes it difficult to further improve the soothing and redness-removing properties of Bordeaux leaf extracts.

[0025] Carboxymethyl chitosan is characterized by film-forming, water-retaining and soothing effects. It can form a water-retaining film on the skin surface, and its polysaccharide structure can effectively load volatile oils, flavonol glycosides and glycerol glucosides.

[0026] Glyceryl glucoside is characterized by its water-retaining and penetration-promoting effects. On the one hand, it improves the skin's water-retaining effect, providing a foundation for the active performance of Bordeaux leaf extract on the skin. On the other hand, it can combine with glyceryl glucoside to improve the penetration effect of glyceryl glucoside.

[0027] Combining the properties of the three substances, we surprisingly found that the redness-removing and soothing effect of the combination of the present invention was substantially improved compared with the Bordeaux leaf extract alone.

[0028] In the process of raw material screening, we screened a large number of plant ingredients with soothing effects, such as Chondrus crissus extract and Paeonia albiflora flower extract. These plant extracts have a relatively clear redness-removing and soothing effect; for example, Chondrus crissus extract contains rich polysaccharides and other beneficial ingredients. These substances have a good calming and soothing effect, which can relieve skin sensitivity and discomfort. For symptoms such as skin redness, swelling, and itching, Chondrus crissus can quickly relieve and repair damaged skin barriers; Paeonia albiflora flower extract can soothe skin sensitivity and discomfort. It is rich in flavonoids, which have anti-inflammatory properties and can inhibit inflammatory reactions in the body. They have a certain effect on relieving skin inflammation and can promote the repair and protection of the skin epidermis. Sensitive skin can effectively reduce the growth of bacteria on the skin, reduce the risk of inflammation and infection, and reduce external stimulation and bacterial invasion by using skin care products containing peony flower extract.

[0029] Under the action of the film-forming agent, the plant extracts mentioned above can also show soothing and synergistic results; however, in terms of the comprehensive soothing effect, the Bordeaux leaf extract has the greatest improvement, and this case is proposed based on this.

[0030] The above-mentioned fast-acting redness-removing and soothing composition includes the following components in parts by weight:

[0031] 8-12 parts of Bordeaux leaf extract;

[0032] 2-4 parts of carboxymethyl chitosan;

[0033] Glyceryl glucoside 22-28 parts.

[0034] At the same time, the present invention also discloses the use of the above-mentioned quick-acting redness-removing and soothing composition for preparing cosmetics.

[0035] Finally, the present invention also discloses a cosmetic with redness-removing and soothing functions, comprising the above-mentioned quick-acting redness-removing and soothing composition.

[0036] In the above-mentioned cosmetics, the concentration of the fast-acting redness-removing and soothing composition is 1 to 10 wt %.

[0037] This application has at least the following beneficial effects:

[0038] The composition of the present invention uses Bordeaux leaf extract as the main active ingredient, carboxymethyl chitosan and glycerol glucoside as moisturizing and penetration-promoting active ingredients, and is supplemented with the additional soothing function of carboxymethyl chitosan to achieve the optimization of soothing and redness-removing effects. BRIEF DESCRIPTION OF THE DRAWINGS

[0039] Figure 1 This is a graph of the zebrafish embryo blood circulation test results of Example 2. DETAILED DESCRIPTION

[0040] Below in conjunction with embodiments of the present invention, the present invention is clearly and completely described. In the description of the present invention, it should be noted that, in the embodiments, those not indicating specific conditions, are carried out according to the conditions of normal conditions or manufacturer's suggestions. Reagents used or instruments not indicating manufacturers are conventional products that can be obtained by commercial purchase. In the case of not making special instructions, the parts used in the embodiments of the present invention are all parts by weight.

[0041] Source of raw materials

[0042] Bordeaux leaf extract: A00066 BETAPUR PGF from BASF (China) Co., Ltd., with an active ingredient content of 7.5wt%;

[0043] Carboxymethyl chitosan: source: Shanghai Chunlu Biochemical Co., Ltd. MHAMicro (version 5.2), active ingredient content 100wt%;

[0044] Glycerol glucoside: source: Shanghai Xuntian Biotechnology Co., Ltd., glycerol glucoside content 45wt%;

[0045] Chondrus Crispus Extract: Satiagum from Cargill France SAS TM VPC 430, active ingredient content 100wt%;

[0046] Peony (PAEONIA ALBIFLORA) flower extract: Peony flower extract from Shanghai Xuntian Biotechnology Co., Ltd., with an active ingredient content of 3.5wt%.

[0047] Part I Preparation and Efficacy Verification of Composition

[0048] Examples and Comparative Examples

[0049] The components shown in Table 1 are mixed to form a homogeneous phase, wherein butanediol and hexylene glycol are present as solvents with functions such as moisturizing and promoting penetration, and cinnamic acid is used as a preservative. They are all commonly used reagents in the art and do not play a decisive role in the efficacy and verification of the composition of the present invention.

[0050] Table 1 Formula Unit: g

[0051]

[0052]

[0053] Performance Testing

[0054] The test items of the present invention include safety test, hyaluronidase inhibition rate test, human lactic acid sting test method, and zebrafish embryo blood circulation test (this test is sent to a third party for testing).

[0055] Multiple skin irritation tests

[0056] Detection method:

[0057] Test substance: Take an appropriate amount of the sample in Example 3 and mix thoroughly at a ratio of 1:19 (sample: pure water, volume ratio) as the test substance.

[0058] Experimental animals: 4 ordinary New Zealand rabbits;

[0059] 24h before the test, the hair on both sides of the back of the New Zealand rabbit was cut off, and the hair removal area on both sides was about 3cm×3cm, with the right side as the coating area and the left side as the blank control area. 0.5mL of the sample of Example 7 was measured respectively and evenly applied to the skin of the animal coating area, once a day, for 14 consecutive days. Starting from the second day, the hair was cut before each coating, and the residual test substance was removed with pure water for 1h and the results were observed. The scores were scored according to the skin irritation / corrosion test table 1 of the "Safety Technical Specifications for Cosmetics" (2015 edition), and the average score of each animal per day was calculated according to the following formula, and the skin irritation intensity was determined according to the skin irritation / corrosion test table 2 of the "Safety Technical Specifications for Cosmetics" (2015 edition).

[0060] Average score per animal per day = (∑ erythema and edema score / number of animals tested) / 14

[0061] The experimental results are shown in Table 2;

[0062] Table 2 Irritation test results

[0063]

[0064] Conclusion

[0065] The sample of Example 3 showed no irritation to the skin of New Zealand rabbits after multiple tests.

[0066] 2. Hyaluronidase inhibition rate test

[0067] 2.1 Experimental principles and basis

[0068] Hyaluronidase is a hydrolase that degrades hyaluronic acid. It is the main component of the extracellular matrix of the body's connective tissue and is related to most IgE-mediated type I and T-cell-mediated type IV allergic reactions. Therefore, the hyaluronidase in vitro inhibition test can reflect the soothing, anti-allergic, and anti-inflammatory effects of the corresponding product on the skin to a certain extent. Hyaluronic acid is a high-grade polysaccharide composed of D-glucuronic acid and N-acetylglucosamine, which can undergo a color reaction according to the Elson-morgan reaction. The inhibition rate of hyaluronidase in the sample can be determined by this reaction.

[0069] This test determines the inhibition rate of hyaluronidase in the sample according to the test method of "Detailed Test Rules for Verification of Soothing Efficacy of Cosmetics" and determines whether the sample has soothing effect by comparing it with the negative control.

[0070] 2.2 Experimental methods

[0071] 2.2.1 Treatment of control and experimental samples

[0072] Experimental sample: Dilute with pure water to a sample concentration of 10%;

[0073] Negative control: pure water.

[0074] 2.2.2 Experimental procedures

[0075] Weigh the samples accurately and perform extraction pretreatment on the samples;

[0076] The pretreated samples were operated according to the modified Elson-morgan reaction, and a negative control was set up;

[0077] Follow the test kit to measure the OD value;

[0078] The hyaluronidase inhibition rate was calculated.

[0079] 2.3 Correlation between experimental indicators and soothing effects

[0080] Inhibition rate (%) = (1-T / C) × 100

[0081] T – the content of hyaluronidase measured after the test; C – the content of hyaluronidase added before the test; The test results are characterized by the inhibition rate of the sample on hyaluronidase, which is compared with the negative control with soothing effect. If the test sample group has an inhibition rate compared with the negative control, and the test sample group has a significant difference compared with the negative control, it means that the test sample group has a soothing effect.

[0082] The test results are shown in Table 3 below;

[0083] Table 3 Hyaluronidase test results

[0084] Hyaluronidase inhibition rate % Example 1 50.28±1.36 Example 2 54.48±1.94 Example 3 63.68±1.59 Example 4 51.16±1.96 Example 5 63.12±1.68 Comparative Example 1 45.67±1.64 Comparative Example 2 38.36±1.36 Comparative Example 3 35.27±1.65 Comparative Example 4 30.48±1.73 Comparative Example 5 34.36±1.62 Comparative Example 6 31.25±1.46 Negative control 1.39±0.13

[0085] Result analysis:

[0086] 1. It can be seen from the test results of Examples 1 to 3 that with the increase of each effective ingredient, the hyaluronic acid inhibition rate increases significantly; based on the results of Examples 1 to 5, it can be seen that the effect of Example 3 of the present invention is the best;

[0087] At the same time, it can be seen from the test results of Examples 1 to 5 that the Bordeaux leaf extract has a more significant effect on the hyaluronic acid inhibition rate. When the amount of Bordeaux leaf extract exceeds 10%, the hyaluronic acid inhibition rate is significantly improved. We believe that the Bordeaux leaf extract is a more critical factor in improving the performance of the composition.

[0088] 2. It can be seen from the test results of Example 2, Comparative Example 1 and Comparative Example 2 that, although the importance of Bordeaux leaf extract is generally proved in the above discussion, the experiment of Comparative Example 1 shows that Bordeaux leaf extract and glycerol glucoside have a good soothing effect, and the experiment of Comparative Example 2 shows that Bordeaux leaf extract alone also has a good soothing effect. On the whole, the soothing effect is further improved when glycerol glucoside and Bordeaux leaf extract are used; the soothing effect is significantly improved when glycerol glucoside, carboxymethyl deacetylated chitosan and Bordeaux leaf extract are used;

[0089] 3. It can be seen from the test results of Example 2, Comparative Example 2, Comparative Example 3, Comparative Example 4, Comparative Example 5 and Comparative Example 6 that the Bordeaux leaf extract, Chondrus crispus extract and Paeonia lactiflora flower extract all have good effects. Under the same dosage, carboxymethyl deacetylated chitosan and glycerol glucoside have a more obvious synergistic effect on the improvement of the soothing effects of the three matrices.

[0090] Human Experimental Soothing Experiment: Histamine Modeling-Redness and Itch Experiment Basis

[0091] 3.1 Mechanism of action of histamine

[0092] Histamine activates transient receptor potential receptors, triggering intracellular signal transduction and leading to an itch response. This effect is independent of nerve agents and is achieved directly through the release of histamine from mast cells.

[0093] 3.2 Experimental methods

[0094] Subjects and number: 15 adults aged 18-55, both male and female;

[0095] Test site: Inside of the front of the arm

[0096] Environmental conditions: Temperature: 25±1℃, Humidity: 50±10%

[0097] Reagent materials and instruments: histamine phosphate, medical pricking needles, soothing samples;

[0098] Test method (puncture method): Use a puncture needle to gently puncture the test area on the inside of the arm, with no bleeding as the standard; use 1% histamine phosphate solution, drip 0.02ml on the inside of the arm, absorb the histamine solution after 5 minutes, and wait for about 10 minutes. Apply 0.02g of sample to the 2*2cm area of ​​the sample area and the control area, record the redness and itching of the arm at each time point, and score and record. The assessor evaluates the test site at the corresponding time points 0min, 5min, and 15min.

[0099] Histamine pain improvement rate (%) = (pain score at 0 min after histamine pricking modeling - pain score at n minutes after histamine pricking modeling) × 100% / pain score at 0 min after histamine pricking modeling

[0100] Histamine redness improvement rate (%) = (redness score at 0 min after histamine pricking modeling - redness score at n minutes after histamine pricking modeling) × 100% / redness score at 0 min after histamine pricking modeling

[0101] Note: ① Itch / pain scoring criteria:

[0102] 5 points: obvious severe itching / pain, can't help scratching / unbearable;

[0103] 4 points: obvious itching / pain, want to scratch from time to time / can tolerate it;

[0104] 3 points: slight itching / pain, no treatment is required;

[0105] 2 points: vague itching / pain, which cannot be accurately described;

[0106] 1 point: No itching or pain.

[0107] ②Redness Scoring Criteria:

[0108] 5 points: obvious and severe redness;

[0109] 4 points: obvious redness;

[0110] 3 points: moderate redness;

[0111] 2 points: very slight redness;

[0112] 1 point: No redness, same skin color as untreated area.

[0113] The test results are shown in Table 4 below;

[0114] Table 4 Histamine phosphate stimulation soothing redness test results

[0115]

[0116]

[0117] Result analysis:

[0118] It can be seen from the above experimental results that the formula of the present invention is more effective in improving skin irritation by using carboxymethyl chitosan, glycerol glucoside and three different matrices.

[0119] 4. Zebrafish Embryo Blood Circulation Test

[0120] The sample of Example 2 of the present invention was tested for blood circulation in zebrafish embryos, and the test results showed that Figure 1 ;

[0121] Test method: 20 3-day-old zebrafish embryos were exposed to 5g / L redness-removing composition sample solution, and a blank control group was set up. After 2 hours of exposure, the tail aorta of each zebrafish embryo was photographed under a microscope for 20 seconds. Then the blood flow velocity in the tail aorta of each fish embryo was analyzed by software, and the average value was taken and statistical analysis was performed;

[0122] Test results: At a test concentration of 5 g / L, the sample had a 15% reduction rate in blood circulation in the caudal aorta of zebrafish embryos (p=0.0000000044).

[0123] Conclusion: The sample can significantly slow down the blood flow velocity of zebrafish embryos and has the effect of slowing down blood circulation, which can serve as supporting evidence for the efficacy of Shuhong.

[0124] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above and that the present invention can be implemented in other specific forms without departing from the spirit or essential features of the present invention. Therefore, the embodiments should be considered exemplary and non-limiting in all respects, and the scope of the present invention is defined by the appended claims rather than the above description, and it is intended that the equivalents falling within the claims be included. All changes in the meaning and scope of the elements are encompassed by the present invention. Any reference numeral in a claim should not be considered as limiting the claim to which it relates.

Claims

1. A fast-acting redness-removing and soothing composition, characterized in that: It includes the following components by weight: 5-15 parts of Bordeaux leaf extract; 1-5 parts of carboxymethyl chitosan; Glyceryl glucoside 20-30 parts.

2. The fast-acting redness-removing and soothing composition according to claim 1, characterized in that: It includes the following components by weight: 8-12 parts of Bordeaux leaf extract; 2-4 parts of carboxymethyl chitosan; Glyceryl glucoside 22-28 parts.

3. Use of the fast-acting redness-removing and soothing composition as claimed in claim 1 or 2 in preparing cosmetics.

4. A cosmetic with redness-removing and soothing function, characterized in that: Contains the fast-acting redness-removing and soothing composition as claimed in claim 1 or 2.

5. The cosmetic according to claim 4, characterized in that: In the cosmetic, the concentration of the fast-acting redness-removing and soothing composition is 1 to 10 wt %.

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