Composition with tightening and anti-aging effects and application of composition in cosmetics
Patent Information
- Application Number
- CN202510232605.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-02-28
- Publication Date
- 2025-05-23
AI Technical Summary
The compositions used in existing cosmetics for anti-aging and firming skin have problems of complex composition, poor stability and short-lasting efficacy, which limit their wide application.
The synergistic effect between rattan tea extract, nude calcium polysaccharide, hydrolyzed collagen, acetyl hydroxyproline and polyaspartate is adopted to form a film instantly through instant filming of nude calcium polysaccharide, hydrolyzed collagen and polyaspartate, and the skin's firmness and elasticity are comprehensively improved through the lipid-absorbing function of rattan tea extract and the moisturizing and repairing effect of acetyl hydroxyproline.
It achieves the improvement of skin firmness and elasticity from multiple dimensions, reduces wrinkles, and achieves the purpose of anti-aging and skin care. It also has the advantages of stable activity and long-lasting effects.
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Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of cosmetics, and in particular relates to a composition with firming and anti-aging effects and application thereof in cosmetics. Background Art
[0002] With the acceleration of the pace of modern life and the increase of environmental pressure, skin aging has become a topic of widespread concern worldwide. As the largest organ of the human body, the skin not only bears the important function of protecting internal tissues, but also directly affects personal appearance and self-confidence. However, in today's society, multiple factors such as ultraviolet radiation, air pollution, exposure to blue light from electronic devices, and irregular lifestyles and eating habits have accelerated the aging process of the skin, leading to a series of problems such as sagging skin, decreased elasticity, increased fine lines and wrinkles. The emergence of these problems not only affects people's appearance, but also poses a potential threat to mental health. Although traditional skin care methods can alleviate these phenomena to a certain extent, their effects are often limited and it is difficult to fundamentally improve the skin condition. For example, simple moisturizing or sun protection measures can only delay skin moisture loss or reduce ultraviolet damage in the short term, but cannot fully cope with complex aging problems. In addition, single active ingredients also show obvious limitations in solving multi-dimensional skin aging problems. Some ingredients may focus on a specific function, such as anti-oxidation, promoting collagen production or repairing barrier function, but in the face of complex situations where multiple aging factors work together, single ingredients often perform poorly. Therefore, developing a combination of multiple raw materials has become an important trend in the current cosmetics research and development field. By scientifically and rationally matching active ingredients with different functions, the combination can achieve a synergistic effect, thereby achieving more significant firming and anti-aging effects.
[0003] Chinese patent CN105055188A discloses a composition with anti-aging effect and its preparation method and application. The composition is composed of the following ingredients: hydrolyzed chitosan, fish collagen peptide, glucosamine, propylene glycol, methyl paraben, phenoxyethanol, water. The composition can resist aging from the aspects of moisturizing, supplementing collagen and removing free radicals through the combination of various components, and achieve the effects of wrinkle removal, repair and skin improvement; Chinese patent CN107308101A discloses a skin firming composition and its application. The composition is composed of the following ingredients: plankton extract, hydrolyzed collagen, dipalmitoyl hydroxyproline, composite amino acids, hyaluronic acid, decarboxylation Peptides, acetylated hexapeptides, lactobacillus / soy milk fermentation product filtrate, dipotassium glycyrrhizinate, retinol palmitate, VE and hydrolyzed sesame protein. The composition uses anti-aging vitamins, amino acids and derivatives, and peptides to endogenously improve the extracellular matrix structure, repair wrinkles and fine lines caused by skin sagging, and improve skin firmness and elasticity by promoting the synthesis of collagen and elastin. Although the composition disclosed in the above patent can play a certain anti-aging and skin firming effect, there are still some problems to be improved, such as the relatively complex composition of the composition, the poor stability of the composition, and the short-lasting effect, which limits the wide application of the composition. Summary of the invention
[0004] In view of the shortcomings of the prior art, the present invention provides a composition with firming and anti-aging effects. By adopting the synergistic effect between a specific rattan tea extract and fine algae polysaccharides, hydrolyzed collagen, acetylhydroxyproline and sodium polyaspartate, the skin's firmness and elasticity can be comprehensively improved from multiple dimensions, wrinkles can be reduced, and the purpose of anti-aging and skin care can be achieved. At the same time, it has the advantages of stable activity and long-lasting efficacy.
[0005] The technical solution adopted by the present invention is as follows:
[0006] The present invention provides a composition with firming and anti-aging effects, comprising the following raw materials in parts by weight:
[0007] 1-20 parts of rattan tea extract, 0.1-10 parts of fine algae polysaccharide, 1-15 parts of hydrolyzed collagen, 0.1-10 parts of acetylhydroxyproline, and 0.1-10 parts of sodium polyaspartate.
[0008] Preferably, a composition with firming and anti-aging effects is composed of the following raw materials in parts by weight:
[0009] 5-8 parts of rattan tea extract, 1-5 parts of fine algae polysaccharide, 4-7 parts of hydrolyzed collagen, 1-4 parts of acetylhydroxyproline, and 1-3 parts of sodium polyaspartate.
[0010] Tengcha, the name of traditional Chinese medicine, is the branches and leaves of Ampelopsis megalophylla Diels et Gilg, a plant of the grape family. Tengcha extract is rich in flavonoids, polyphenols and other active ingredients, among which dihydromyricetin is one of its representative highly active ingredients. Dihydromyricetin not only has significant antioxidant, anti-inflammatory, anti-cancer, hypoglycemic and hypolipidemic effects, but also plays a certain role in the regulation of fat metabolism. Studies have shown that dihydromyricetin can effectively inhibit the differentiation process of white fat cells, promote the transformation of white fat into brown fat, and enhance the heat production function of brown fat, thereby improving the overall distribution and metabolic state of fat. Based on this, tengcha extract can help reduce excess facial fat accumulation by regulating facial fat metabolism, and alleviate the problem of blurred facial contours caused by excessive fat deposition. In addition, its rich antioxidant ingredients can protect the skin from free radical damage, promote collagen synthesis, and improve skin elasticity and firmness, ultimately achieving the goal of optimizing facial lines and shaping a small face.
[0011] Euglena microalgae polysaccharide is a natural active substance extracted from the unicellular algae Euglena microalgae, and is a type of plant mucopolysaccharide. As a multifunctional bioactive ingredient, it has certain effects in moisturizing, anti-oxidation, anti-inflammatory, and promoting tissue repair and regeneration, so it is widely used in many fields such as medicine, health care products and cosmetics. Its unique molecular structure gives it excellent moisturizing ability, which can form a thin and breathable protective film on the surface of the skin, effectively locking in moisture and improving the dryness of the skin. At the same time, Euglena microalgae polysaccharide can remove free radicals, reduce damage to the skin caused by external environmental factors, and delay the aging process. Its anti-inflammatory properties help relieve skin discomfort caused by external irritation or sensitivity, and the function of promoting repair and regeneration can accelerate the recovery of damaged skin and improve the overall skin quality.
[0012] Collagen is an important protein in the human body. It exists in a triple helix structure and plays a vital role in maintaining the softness, elasticity and structural integrity of tissues (such as skin). As a key component of collagen, hydroxyproline plays a core function in stabilizing the triple helix structure. By forming a hydrogen bond network, it not only enhances the stability of collagen, but also gives it a stronger three-dimensional sense and tensile resistance, thereby effectively improving the elasticity and toughness of the tissue. The hydrolyzed collagen used in the present invention, because of its relatively small molecular weight, can more efficiently penetrate into the deep layers of the skin, provide essential nutrients for the skin, stimulate the synthesis of endogenous collagen and enhance its activity, and this characteristic makes hydrolyzed collagen more easily absorbed and utilized by the skin, thereby showing better effects in improving skin quality, delaying aging and improving skin elasticity.
[0013] Attaching hydrolyzed collagen (molecular weight of about 1000) to tiny algae polysaccharides can form a thin and transparent film on the skin surface. This film can not only quickly play a role in tightening the skin in a short period of time, but also effectively enhance the immediate elasticity of the skin. At the same time, the formation of the film helps the slow release of other ingredients, allowing hydrolyzed collagen to reach deeper into the epidermis, improving its utilization rate and durability. This synergistic effect not only strengthens the nourishing effect of hydrolyzed collagen on the deep layers of the skin, but also significantly prolongs the duration of the moisturizing effect, keeping the skin hydrated, soft and firm for a long time.
[0014] Acetylhydroxyproline is a multifunctional active ingredient that can be used as a skin conditioner, emollient, moisturizer and protectant to provide comprehensive skin care effects. It can effectively increase skin moisture and achieve long-lasting moisturizing effects, while improving skin elasticity, improving fine lines, and delaying the aging process by repairing the skin barrier. Acetylhydroxyproline is a product of acetylated hydroxyproline. Acetylated hydroxyproline can better regulate and control various metabolic pathways in cells and exert its biological effects. Compared with unmodified hydroxyproline, the skin permeability of acetylhydroxyproline is significantly improved, making it easier to be absorbed by the skin and act deeply in the target area, thereby exerting a more efficient and lasting skin care effect.
[0015] Sodium polyaspartate is a biopolymer polypeptide substance formed by the combination of the amino and carboxyl groups of aspartic acid. It exists naturally in the shells of snails and other mollusks. Its molecular structure contains amide bonds and carboxyl groups, which give it good moisturizing properties and chelating effects. Its monomer aspartic acid is a non-essential amino acid for the human body and one of the 20 basic amino acids that make up protein. The sodium polyaspartate synthesized from it is a high-affinity, anionic moisturizing polymer that can form a flexible and breathable film on the surface of the skin and hair through bioadhesion. This bioadhesive film can not only effectively lock in moisture and prevent moisture loss, but also provide a protective barrier for the skin and hair.
[0016] The composition of the present invention is composed of rattan tea extract, fine algae polysaccharide, hydrolyzed collagen, acetylhydroxyproline and sodium polyaspartate. The instant film formation of fine algae polysaccharide, hydrolyzed collagen and sodium polyaspartate can achieve a rapid firming effect, and the fat-removing function of the rattan tea extract is combined to optimize the facial contour, and acetylhydroxyproline and hydrolyzed collagen are used to promote collagen production, thereby comprehensively improving the firmness and elasticity of the skin from multiple dimensions, thereby achieving the purpose of anti-aging and skin care.
[0017] Preferably, the rattan tea extract is prepared by the following method:
[0018] The rattan tea is crushed to obtain rattan tea powder; the rattan tea powder and regulating aid are added into water for ultrasonic treatment, and then a mixed enzyme is added for enzymolysis to obtain an enzymolysis product; fermentation bacteria are inoculated into the enzymolysis product for fermentation to obtain a fermentation product; the fermentation product is filtered, the filtrate is concentrated to obtain an extract I, and the filter residue is reserved; the filter residue is subjected to supercritical extraction, the extract is concentrated to obtain an extract II; the extract I is combined with the extract II to obtain a rattan tea extract.
[0019] The present invention adopts the above-mentioned specific method to prepare the rattan tea extract, which can further improve the antioxidant activity and anti-aging effect of the composition. In the preparation process, the biological activity of the rattan tea extract is improved from the steps of pretreatment, enzymolysis, fermentation to supercritical extraction. Among them, a regulating auxiliary agent composed of D-sorbitol and Tween-20 is added during the ultrasonic treatment process. D-sorbitol, as an osmotic pressure regulator, can stabilize the osmotic pressure difference between the inside and outside of the plant cells, promote the release of cell contents, and provide a stable environment for the enzymolysis reaction, avoiding the loss of enzyme activity due to osmotic pressure fluctuations; Tween-20, as a non-ionic surfactant, can reduce the surface tension of the solvent, enhance the contact efficiency of the enzyme and the rattan tea material, and improve the ability of the enzyme to penetrate into the plant cell wall, thereby accelerating the enzymolysis of cellulase, pectinase and papain on the plant material. The synergistic effect of the two not only improves the enzymolysis efficiency, but also improves the activity stability of the target extract. Enzymolysis destroys the structure of the plant cell wall, accelerates the dissolution of intracellular substances, and decomposes the complex polysaccharides in the cell wall into small molecular sugars that are more easily utilized by fermentation bacteria, providing rich nutrients for the subsequent fermentation process. The present invention uses a composite bacteria composed of plant lactobacillus and bacillus amyloliquefaciens for fermentation, giving full play to the advantages of different strains. Plant lactobacillus can produce abundant active extracellular polysaccharides, which can stimulate the proliferation of skin cells, help repair aging damaged skin, and enhance anti-aging effects; Bacillus amyloliquefaciens assists plant lactobacillus in metabolizing rattan tea materials with its strong enzyme production ability, further promoting the generation and enrichment of active ingredients, thereby obtaining a fermentation extract rich in active ingredients. The present invention also performs secondary extraction on the filter residue after fermentation by supercritical extraction technology, and combines an entrainer composed of anhydrous ethanol and water to effectively extract the active ingredients remaining in the filter residue, so that the finally obtained rattan tea extract is more active, showing excellent antioxidant activity and anti-aging effects.
[0020] Preferably, the rattan tea extract is prepared by the following method:
[0021] The rattan tea is crushed to obtain rattan tea powder; 20-30 parts by weight of rattan tea powder and 3-7 parts by weight of a regulating aid are added to 250-350 parts by weight of water for ultrasonic treatment, and then 1.5-3 parts by weight of a mixed enzyme is added for enzymolysis, sterilized, and cooled to obtain an enzymolysis product; fermentation bacteria are inoculated into the enzymolysis product for fermentation, sterilized, and cooled to obtain a fermentation product; the fermentation product is filtered, the filtrate is collected and concentrated to 8-12% of the original volume to obtain an extract I, and the filter residue is reserved; the filter residue is placed in an extraction kettle, an entrainer is added, supercritical extraction is performed, the extract is collected, and the extract is concentrated to 8-12% of the original volume to obtain an extract II; the extract I and the extract II are combined to obtain a rattan tea extract.
[0022] Preferably, the regulating auxiliary agent is a mixture of D-sorbitol and Tween-20; the mass ratio of D-sorbitol to Tween-20 is 1-2:1.
[0023] Preferably, the mixed enzyme is a mixture of cellulase, pectinase and papain; the mass ratio of the cellulase, pectinase and papain is 1:(0.4-0.8):(0.2-0.6).
[0024] Preferably, the fermentation bacteria are Bacillus plantarum and / or Bacillus amyloliquefaciens.
[0025] Preferably, the fermentation bacteria consists of Bacillus plantarum and Bacillus amyloliquefaciens; the mass ratio of Bacillus plantarum to Bacillus amyloliquefaciens is 1-2:1.
[0026] Preferably, the entrainer is a mixture of anhydrous ethanol and water; the mass ratio of the anhydrous ethanol to water is 1:0.2-0.4.
[0027] Preferably, the rattan tea is dried to constant weight and then crushed; after crushing, it is sieved through a 50-80 mesh sieve.
[0028] Preferably, the power of the ultrasound is 100-200 W, the frequency is 25-40 kHz, and the time is 15-30 min.
[0029] Preferably, the enzymatic hydrolysis temperature is 48-55°C and the time is 270-330 min.
[0030] Preferably, the pH is adjusted to 5.5-6.5 before enzymolysis and then adjusted to neutral after enzymolysis to obtain an enzymolysis product.
[0031] Preferably, the concentration method is reduced pressure concentration.
[0032] Preferably, the cooling is cooling to room temperature.
[0033] Preferably, the fermentation temperature is 28-35°C and the fermentation time is 48-72h.
[0034] Preferably, the inoculation amount of the fermentation bacteria is 2-4%.
[0035] Preferably, the sterilization temperature is 115-121° C. and the time is 15-30 min.
[0036] Preferably, the supercritical extraction pressure is 25-35 MPa, the temperature is 50-65° C., and the time is 90-150 min.
[0037] Preferably, the supercritical extraction medium is CO 2 Fluid; the CO 2 The flow rate is 18-22kg / h.
[0038] Preferably, the amount of the entrainer added is 25-35wt% of the mass of the filter residue.
[0039] The invention also discloses application of the above-mentioned composition with firming and anti-aging effects in preparing cosmetics.
[0040] Preferably, the cosmetic is any one of essence, toner, skin care lotion, face cream and facial mask.
[0041] Preferably, the amount of the composition with firming and anti-aging effects in cosmetics is 0.5-15wt%.
[0042] Preferably, the amount of the composition with firming and anti-aging effects in cosmetics is 1-10 wt %.
[0043] Beneficial effects of the present invention:
[0044] 1. The present invention provides a composition with firming and anti-aging effects, which is composed of vine tea extract, fine algae polysaccharide, hydrolyzed collagen, acetylhydroxyproline and sodium polyaspartate. The composition achieves a rapid firming effect through instant film formation of fine algae polysaccharide, hydrolyzed collagen and sodium polyaspartate, optimizes facial contours in combination with the fat-removing function of vine tea extract, and promotes collagen production with the help of acetylhydroxyproline and hydrolyzed collagen, comprehensively improving the firmness and elasticity of the skin from multiple dimensions, achieving the purpose of anti-aging and skin care, and having the advantages of stable activity and lasting efficacy. The present invention also provides the use of the above composition in the preparation of cosmetics.
[0045] 2. In the above-mentioned composition provided by the present invention, the fine algae polysaccharide has moisturizing, anti-oxidation, anti-inflammatory and other effects, and can form a thin and breathable protective film on the skin surface, effectively locking in moisture and improving the dry state of the skin, and delaying aging; small molecule hydrolyzed collagen can penetrate into the deep layer of the skin more efficiently, providing the skin with essential nutrients, while stimulating the synthesis of endogenous collagen, thereby showing better effects in improving skin quality, delaying aging, and enhancing skin elasticity; sodium polyaspartate can form a thin film on the skin surface through biological adhesion, which can not only effectively lock in moisture and prevent moisture loss, but also provide a protective barrier for the skin.
[0046] 3. In the above-mentioned composition provided by the present invention, the rattan tea extract can help reduce excess fat accumulation on the face by regulating facial fat metabolism, and can also protect the skin from free radical damage, promote collagen synthesis, and improve skin elasticity and firmness, ultimately achieving the goal of optimizing facial lines and shaping a small face effect; acetylhydroxyproline can effectively increase skin moisture and achieve long-lasting moisturizing effects, while improving skin elasticity, improving fine lines, and delaying the aging process by repairing the skin barrier. The acetylated hydroxyproline can better regulate and control various metabolic pathways in the cell and exert its biological effects. DETAILED DESCRIPTION
[0047] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.
[0048] Euglena polysaccharide was purchased from Xi'an Huaxing Biotechnology Co., Ltd., model: LZDT-38, purity: 98%. Hydrolyzed collagen was purchased from Xiamen Haiyou Gelatin Co., Ltd. (full name: hydrolyzed fish collagen), average molecular weight: 1000, purity: 96%. Acetylhydroxyproline was purchased from Shaanxi Moore Biotechnology Co., Ltd. (full name: N-acetyl-L-4-hydroxyproline), purity: 99%. Sodium polyaspartate was purchased from Hunan Deweier New Materials Co., Ltd., solid content: 20wt%, average molecular weight: 5000. Cellulase was purchased from Shanghai Titan Technology Co., Ltd., enzyme activity: 10000U / g. Pectinase was purchased from Shanghai Yien Chemical Technology Co., Ltd., enzyme activity: 30000U / g. Papain was purchased from Xi'an Kangnuo Chemical Co., Ltd., enzyme activity: 200000U / g. Plant lactobacillus was purchased from China Industrial Microbiological Culture Collection Management Center, strain number: CICC 20272. Bacillus amyloliquefaciens was purchased from China Industrial Microbiological Culture Collection Center, strain number: CICC 21746.
[0049] Example 1
[0050] A composition with firming and anti-aging effects, comprising the following raw materials in parts by weight:
[0051] 6.5 parts by weight of rattan tea extract, 3.5 parts by weight of fine algae polysaccharide, 5.5 parts by weight of hydrolyzed collagen, 2.5 parts by weight of acetylhydroxyproline, and 2 parts by weight of sodium polyaspartate.
[0052] The rattan tea extract is prepared by the following method:
[0053] Dry the rattan tea to constant weight, crush and pass through a 60-mesh sieve to obtain rattan tea powder; add 25 parts by weight of rattan tea powder and 5 parts by weight of a regulating aid to 200 parts by weight of water, ultrasonicate at 150W and 30kHz for 20 minutes, then add 2 parts by weight of a mixed enzyme, adjust the pH to 6.0, perform enzymolysis at 50°C for 300 minutes, sterilize at 121°C for 20 minutes after the enzymolysis, cool to room temperature, adjust the pH to neutral, and obtain an enzymolysis product; wherein the regulating aid is a mixture of D-sorbitol and Tween-20 in a mass ratio of 1.5:1; the mixed enzyme is a mixture of cellulase, pectinase and papain in a mass ratio of 1:0.6:0.4;
[0054] Inoculate fermentation bacteria into the above enzymatic hydrolysate, ferment at 30°C for 60h, sterilize at 121°C for 20min after the fermentation, and cool to room temperature to obtain a fermentation product; filter the fermentation product, collect the filtrate, decompress and concentrate it to 10% of the original volume to obtain extract I, and use the filter residue for later use; wherein the inoculation amount of the fermentation bacteria is 3%; the fermentation bacteria is composed of Bacillus plantarum and Bacillus amyloliquefaciens in a mass ratio of 1.5:1;
[0055] The above-mentioned filter residue was placed in an extraction kettle, and an entrainer was added. CO 2 The supercritical extraction was performed for 120 minutes, and the extract was collected and concentrated under reduced pressure to 10% of the original volume to obtain extract II; the extract I and extract II were combined to obtain the rattan tea extract. 2 The flow rate is 20kg / h, the entrainer is a mixture of anhydrous ethanol and water in a mass ratio of 1:0.25; the amount of the entrainer added is 30wt% of the mass of the filter residue.
[0056] Example 2
[0057] A composition with firming and anti-aging effects, comprising the following raw materials in parts by weight:
[0058] 5 parts by weight of rattan tea extract, 1 part by weight of fine algae polysaccharide, 4 parts by weight of hydrolyzed collagen, 1 part by weight of acetylhydroxyproline, and 1 part by weight of sodium polyaspartate.
[0059] The rattan tea extract is prepared by the following method:
[0060] Dry the rattan tea to constant weight, crush and pass through a 50-mesh sieve to obtain rattan tea powder; add 20 parts by weight of rattan tea powder and 3 parts by weight of a regulating aid to 250 parts by weight of water, ultrasonicate at 100W and 25kHz for 30 minutes, then add 1.5 parts by weight of a mixed enzyme, adjust the pH to 5.5, perform enzymolysis at 48°C for 330 minutes, sterilize at 115°C for 30 minutes after the enzymolysis, cool to room temperature, adjust the pH to neutral, and obtain an enzymolysis product; wherein the regulating aid is a mixture of D-sorbitol and Tween-20 in a mass ratio of 1:1; the mixed enzyme is a mixture of cellulase, pectinase and papain in a mass ratio of 1:0.4:0.6;
[0061] Inoculate fermentation bacteria into the above enzymatic hydrolysate, ferment at 28°C for 72h, sterilize at 115°C for 30min after the fermentation, and cool to room temperature to obtain a fermentation product; filter the fermentation product, collect the filtrate, decompress and concentrate it to 8% of the original volume to obtain extract I, and use the filter residue for later use; wherein the inoculation amount of the fermentation bacteria is 2%; the fermentation bacteria is composed of Bacillus plantarum and Bacillus amyloliquefaciens in a mass ratio of 1:1;
[0062] The above-mentioned filter residue was placed in an extraction kettle, and an entrainer was added. CO 2 The supercritical extraction was performed for 150 minutes, and the extract was collected and concentrated under reduced pressure to 8% of the original volume to obtain extract II; the extract I and extract II were combined to obtain the rattan tea extract. 2 The flow rate is 18kg / h, the entrainer is a mixture of anhydrous ethanol and water in a mass ratio of 1:0.2; the amount of the entrainer added is 25wt% of the mass of the filter residue.
[0063] Example 3
[0064] A composition with firming and anti-aging effects, comprising the following raw materials in parts by weight:
[0065] 8 parts by weight of rattan tea extract, 5 parts by weight of fine algae polysaccharide, 7 parts by weight of hydrolyzed collagen, 4 parts by weight of acetylhydroxyproline, and 3 parts by weight of sodium polyaspartate.
[0066] The rattan tea extract is prepared by the following method:
[0067] Dry the rattan tea to constant weight, crush and pass through an 80-mesh sieve to obtain rattan tea powder; add 30 parts by weight of rattan tea powder and 7 parts by weight of a regulating aid to 350 parts by weight of water, ultrasonicate at 200W and 40kHz for 15 minutes, then add 3 parts by weight of a mixed enzyme, adjust the pH to 6.5, perform enzymolysis at 55°C for 270 minutes, sterilize at 121°C for 15 minutes after the enzymolysis, cool to room temperature, adjust the pH to neutral, and obtain an enzymolysis product; wherein the regulating aid is a mixture of D-sorbitol and Tween-20 in a mass ratio of 2:1; the mixed enzyme is a mixture of cellulase, pectinase and papain in a mass ratio of 1:0.8:0.2;
[0068] Inoculate fermentation bacteria into the above enzymatic hydrolysate, ferment at 35°C for 48h, sterilize at 121°C for 15min after the fermentation, and cool to room temperature to obtain a fermentation product; filter the fermentation product, collect the filtrate, decompress and concentrate it to 12% of the original volume to obtain extract I, and use the filter residue for later use; wherein the inoculation amount of the fermentation bacteria is 4%; the fermentation bacteria is composed of Bacillus plantarum and Bacillus amyloliquefaciens in a mass ratio of 2:1;
[0069] The above-mentioned filter residue was placed in an extraction kettle, and an entrainer was added. CO 2 Supercritical extraction was performed for 90 minutes, and the extract was collected and concentrated under reduced pressure to 12% of the original volume to obtain extract II; the extract I and extract II were combined to obtain the rattan tea extract. 2 The flow rate is 22kg / h, the entrainer is a mixture of anhydrous ethanol and water in a mass ratio of 1:0.4; the amount of the entrainer added is 35wt% of the mass of the filter residue.
[0070] Example 4
[0071] A composition with firming and anti-aging effects, comprising the following raw materials in parts by weight:
[0072] 6.5 parts by weight of rattan tea extract, 3.5 parts by weight of fine algae polysaccharide, 5.5 parts by weight of hydrolyzed collagen, 2.5 parts by weight of acetylhydroxyproline, and 2 parts by weight of sodium polyaspartate.
[0073] The rattan tea extract is prepared by the following method:
[0074] Dry the rattan tea to constant weight, crush and pass through a 60-mesh sieve to obtain rattan tea powder; add 25 parts by weight of the rattan tea powder to 200 parts by weight of water, then add 2 parts by weight of mixed enzymes, adjust the pH to 6.0, perform enzymolysis at 50°C for 300 minutes, sterilize at 121°C for 20 minutes after the enzymolysis, cool to room temperature, adjust the pH to neutral, and obtain an enzymolysis product; wherein the mixed enzyme is a mixture of cellulase, pectinase and papain in a mass ratio of 1:0.6:0.4;
[0075] Inoculate fermentation bacteria into the above enzymatic hydrolysate, ferment at 30°C for 60h, sterilize at 121°C for 20min after the fermentation, and cool to room temperature to obtain a fermentation product; filter the fermentation product, collect the filtrate, decompress and concentrate it to 10% of the original volume to obtain extract I, and use the filter residue for later use; wherein the inoculation amount of the fermentation bacteria is 3%; the fermentation bacteria is composed of Bacillus plantarum and Bacillus amyloliquefaciens in a mass ratio of 1.5:1;
[0076] The above-mentioned filter residue was placed in an extraction kettle, and an entrainer was added. CO 2 The supercritical extraction was performed for 120 minutes, and the extract was collected and concentrated under reduced pressure to 10% of the original volume to obtain extract II; the extract I and extract II were combined to obtain the rattan tea extract. 2 The flow rate is 20kg / h, the entrainer is a mixture of anhydrous ethanol and water in a mass ratio of 1:0.25; the amount of the entrainer added is 30wt% of the mass of the filter residue.
[0077] Example 5
[0078] A composition with firming and anti-aging effects, comprising the following raw materials in parts by weight:
[0079] 6.5 parts by weight of rattan tea extract, 3.5 parts by weight of fine algae polysaccharide, 5.5 parts by weight of hydrolyzed collagen, 2.5 parts by weight of acetylhydroxyproline, and 2 parts by weight of sodium polyaspartate.
[0080] The rattan tea extract is prepared by the following method:
[0081] Dry the rattan tea to constant weight, crush and pass through a 60-mesh sieve to obtain rattan tea powder; add 25 parts by weight of rattan tea powder and 5 parts by weight of a regulating aid to 200 parts by weight of water, ultrasonicate at 150W and 30kHz for 20 minutes, then add 2 parts by weight of a mixed enzyme, adjust the pH to 6.0, perform enzymolysis at 50°C for 300 minutes, sterilize at 121°C for 20 minutes after the enzymolysis, cool to room temperature, adjust the pH to neutral, and obtain an enzymolysis product; wherein the regulating aid is D-sorbitol; the mixed enzyme is a mixture of cellulase, pectinase and papain in a mass ratio of 1:0.6:0.4;
[0082] Inoculate fermentation bacteria into the above enzymatic hydrolysate, ferment at 30°C for 60h, sterilize at 121°C for 20min after the fermentation, and cool to room temperature to obtain a fermentation product; filter the fermentation product, collect the filtrate, decompress and concentrate it to 10% of the original volume to obtain extract I, and use the filter residue for later use; wherein the inoculation amount of the fermentation bacteria is 3%; the fermentation bacteria is composed of Bacillus plantarum and Bacillus amyloliquefaciens in a mass ratio of 1.5:1;
[0083] The above-mentioned filter residue was placed in an extraction kettle, and an entrainer was added. CO 2 The supercritical extraction was performed for 120 minutes, and the extract was collected and concentrated under reduced pressure to 10% of the original volume to obtain extract II; the extract I and extract II were combined to obtain the rattan tea extract. 2 The flow rate is 20kg / h, the entrainer is a mixture of anhydrous ethanol and water in a mass ratio of 1:0.25; the amount of the entrainer added is 30wt% of the mass of the filter residue.
[0084] Example 6
[0085] A composition with firming and anti-aging effects, comprising the following raw materials in parts by weight:
[0086] 6.5 parts by weight of rattan tea extract, 3.5 parts by weight of fine algae polysaccharide, 5.5 parts by weight of hydrolyzed collagen, 2.5 parts by weight of acetylhydroxyproline, and 2 parts by weight of sodium polyaspartate.
[0087] The rattan tea extract is prepared by the following method:
[0088] Dry the rattan tea to constant weight, crush and pass through a 60-mesh sieve to obtain rattan tea powder; add 25 parts by weight of rattan tea powder and 5 parts by weight of a regulating aid to 200 parts by weight of water, ultrasonicate at 150W and 30kHz for 20 minutes, then add 2 parts by weight of a mixed enzyme, adjust the pH to 6.0, perform enzymolysis at 50°C for 300 minutes, sterilize at 121°C for 20 minutes after the enzymolysis, cool to room temperature, adjust the pH to neutral, and obtain an enzymolysis product; wherein the regulating aid is Tween-20; the mixed enzyme is a mixture of cellulase, pectinase and papain in a mass ratio of 1:0.6:0.4;
[0089] Inoculate fermentation bacteria into the above enzymatic hydrolysate, ferment at 30°C for 60h, sterilize at 121°C for 20min after the fermentation, and cool to room temperature to obtain a fermentation product; filter the fermentation product, collect the filtrate, decompress and concentrate it to 10% of the original volume to obtain extract I, and use the filter residue for later use; wherein the inoculation amount of the fermentation bacteria is 3%; the fermentation bacteria is composed of Bacillus plantarum and Bacillus amyloliquefaciens in a mass ratio of 1.5:1;
[0090] The above-mentioned filter residue was placed in an extraction kettle, and an entrainer was added. CO 2 The supercritical extraction was performed for 120 minutes, and the extract was collected and concentrated under reduced pressure to 10% of the original volume to obtain extract II; the extract I and extract II were combined to obtain the rattan tea extract. 2 The flow rate is 20kg / h, the entrainer is a mixture of anhydrous ethanol and water in a mass ratio of 1:0.25; the amount of the entrainer added is 30wt% of the mass of the filter residue.
[0091] Example 7
[0092] A composition with firming and anti-aging effects, comprising the following raw materials in parts by weight:
[0093] 6.5 parts by weight of rattan tea extract, 3.5 parts by weight of fine algae polysaccharide, 5.5 parts by weight of hydrolyzed collagen, 2.5 parts by weight of acetylhydroxyproline, and 2 parts by weight of sodium polyaspartate.
[0094] The rattan tea extract is prepared by the following method:
[0095] Dry the rattan tea to constant weight, crush and pass through a 60-mesh sieve to obtain rattan tea powder; add 25 parts by weight of rattan tea powder and 5 parts by weight of a regulating aid to 200 parts by weight of water, ultrasonicate at 150W and 30kHz for 20 minutes, then add 2 parts by weight of a mixed enzyme, adjust the pH to 6.0, perform enzymolysis at 50°C for 300 minutes, sterilize at 121°C for 20 minutes after the enzymolysis, cool to room temperature, adjust the pH to neutral, and obtain an enzymolysis product; wherein the regulating aid is a mixture of D-sorbitol and Tween-20 in a mass ratio of 1.5:1; filter the enzymolysis product, collect the filtrate and concentrate it under reduced pressure to 10% of the original volume to obtain extract I, and use the filter residue for later use; the mixed enzyme is a mixture of cellulase, pectinase and papain in a mass ratio of 1:0.6:0.4;
[0096] The above-mentioned filter residue was placed in an extraction kettle, and an entrainer was added. CO 2 The supercritical extraction was performed for 120 minutes, and the extract was collected and concentrated under reduced pressure to 10% of the original volume to obtain extract II; the extract I and extract II were combined to obtain the rattan tea extract. 2 The flow rate is 20kg / h, the entrainer is a mixture of anhydrous ethanol and water in a mass ratio of 1:0.25; the amount of the entrainer added is 30wt% of the mass of the filter residue.
[0097] Example 8
[0098] A composition with firming and anti-aging effects, comprising the following raw materials in parts by weight:
[0099] 6.5 parts by weight of rattan tea extract, 3.5 parts by weight of fine algae polysaccharide, 5.5 parts by weight of hydrolyzed collagen, 2.5 parts by weight of acetylhydroxyproline, and 2 parts by weight of sodium polyaspartate.
[0100] The rattan tea extract is prepared by the following method:
[0101] Dry the rattan tea to constant weight, crush and pass through a 60-mesh sieve to obtain rattan tea powder; add 25 parts by weight of rattan tea powder and 5 parts by weight of a regulating aid to 200 parts by weight of water, ultrasonicate at 150W and 30kHz for 20 minutes, then add 2 parts by weight of a mixed enzyme, adjust the pH to 6.0, perform enzymolysis at 50°C for 300 minutes, sterilize at 121°C for 20 minutes after the enzymolysis, cool to room temperature, adjust the pH to neutral, and obtain an enzymolysis product; wherein the regulating aid is a mixture of D-sorbitol and Tween-20 in a mass ratio of 1.5:1; the mixed enzyme is a mixture of cellulase, pectinase and papain in a mass ratio of 1:0.6:0.4;
[0102] Inoculate fermentation bacteria into the above enzymatic hydrolyzate, ferment at 30°C for 60h, sterilize at 121°C for 20min after the fermentation, and cool to room temperature to obtain a fermentation product; filter the fermentation product, collect the filtrate and concentrate it under reduced pressure to 10% of the original volume to obtain extract I, and use the filter residue for later use; wherein the inoculation amount of the fermentation bacteria is 3%; the fermentation bacteria is Lactobacillus plantarum;
[0103] The above-mentioned filter residue was placed in an extraction kettle, and an entrainer was added. CO 2 The supercritical extraction was performed for 120 minutes, and the extract was collected and concentrated under reduced pressure to 10% of the original volume to obtain extract II; the extract I and extract II were combined to obtain the rattan tea extract. 2 The flow rate is 20kg / h, the entrainer is a mixture of anhydrous ethanol and water in a mass ratio of 1:0.25; the amount of the entrainer added is 30wt% of the mass of the filter residue.
[0104] Example 9
[0105] A composition with firming and anti-aging effects, comprising the following raw materials in parts by weight:
[0106] 6.5 parts by weight of rattan tea extract, 3.5 parts by weight of fine algae polysaccharide, 5.5 parts by weight of hydrolyzed collagen, 2.5 parts by weight of acetylhydroxyproline, and 2 parts by weight of sodium polyaspartate.
[0107] The rattan tea extract is prepared by the following method:
[0108] Dry the rattan tea to constant weight, crush and pass through a 60-mesh sieve to obtain rattan tea powder; add 25 parts by weight of rattan tea powder and 5 parts by weight of a regulating aid to 200 parts by weight of water, ultrasonicate at 150W and 30kHz for 20 minutes, then add 2 parts by weight of a mixed enzyme, adjust the pH to 6.0, perform enzymolysis at 50°C for 300 minutes, sterilize at 121°C for 20 minutes after the enzymolysis, cool to room temperature, adjust the pH to neutral, and obtain an enzymolysis product; wherein the regulating aid is a mixture of D-sorbitol and Tween-20 in a mass ratio of 1.5:1; the mixed enzyme is a mixture of cellulase, pectinase and papain in a mass ratio of 1:0.6:0.4;
[0109] Inoculate fermentation bacteria into the above enzymatic hydrolysate, ferment at 30°C for 60h, sterilize at 121°C for 20min after the fermentation, and cool to room temperature to obtain a fermentation product; filter the fermentation product, collect the filtrate and concentrate it under reduced pressure to 10% of the original volume to obtain extract I, and use the filter residue for later use; wherein the inoculation amount of the fermentation bacteria is 3%; the fermentation bacteria is Bacillus amyloliquefaciens;
[0110] The above-mentioned filter residue was placed in an extraction kettle, and an entrainer was added. CO 2 The supercritical extraction was performed for 120 minutes, and the extract was collected and concentrated under reduced pressure to 10% of the original volume to obtain extract II; the extract I and extract II were combined to obtain the rattan tea extract. 2 The flow rate is 20kg / h, the entrainer is a mixture of anhydrous ethanol and water in a mass ratio of 1:0.25; the amount of the entrainer added is 30wt% of the mass of the filter residue.
[0111] Example 10
[0112] A composition with firming and anti-aging effects, comprising the following raw materials in parts by weight:
[0113] 6.5 parts by weight of rattan tea extract, 3.5 parts by weight of fine algae polysaccharide, 5.5 parts by weight of hydrolyzed collagen, 2.5 parts by weight of acetylhydroxyproline, and 2 parts by weight of sodium polyaspartate.
[0114] The rattan tea extract is prepared by the following method:
[0115] Dry the rattan tea to constant weight, crush and pass through a 60-mesh sieve to obtain rattan tea powder; add 25 parts by weight of rattan tea powder and 5 parts by weight of a regulating aid to 200 parts by weight of water, ultrasonicate at 150W and 30kHz for 20 minutes, then add 2 parts by weight of a mixed enzyme, adjust the pH to 6.0, perform enzymolysis at 50°C for 300 minutes, sterilize at 121°C for 20 minutes after the enzymolysis, cool to room temperature, adjust the pH to neutral, and obtain an enzymolysis product; wherein the regulating aid is a mixture of D-sorbitol and Tween-20 in a mass ratio of 1.5:1; the mixed enzyme is a mixture of cellulase, pectinase and papain in a mass ratio of 1:0.6:0.4;
[0116] Inoculate fermentation bacteria into the above enzymatic hydrolyzate, ferment at 30°C for 60h, sterilize at 121°C for 20min after fermentation, cool to room temperature, and obtain a fermentation product; filter the fermentation product, collect the filtrate, and concentrate it under reduced pressure to 10% of the original volume to obtain a rattan tea extract. The inoculation amount of the fermentation bacteria is 3%; the fermentation bacteria is composed of Bacillus plantarum and Bacillus amyloliquefaciens in a mass ratio of 1.5:1.
[0117] Embodiment 11
[0118] A composition with firming and anti-aging effects, comprising the following raw materials in parts by weight:
[0119] 6.5 parts by weight of rattan tea extract, 5.5 parts by weight of hydrolyzed collagen, 2.5 parts by weight of acetylhydroxyproline, and 2 parts by weight of sodium polyaspartate. The rattan tea extract is the same as that in Example 1.
[0120] Example 12
[0121] A composition with firming and anti-aging effects, comprising the following raw materials in parts by weight:
[0122] 6.5 parts by weight of rattan tea extract, 3.5 parts by weight of fine algae polysaccharide, 2.5 parts by weight of acetylhydroxyproline, and 2 parts by weight of sodium polyaspartate. The rattan tea extract is the same as that in Example 1.
[0123] Example 13
[0124] A composition with firming and anti-aging effects, comprising the following raw materials in parts by weight:
[0125] 6.5 parts by weight of rattan tea extract, 3.5 parts by weight of fine algae polysaccharide, 5.5 parts by weight of hydrolyzed collagen, and 2 parts by weight of sodium polyaspartate. The rattan tea extract is the same as that in Example 1.
[0126] Embodiment 14
[0127] A composition with firming and anti-aging effects, comprising the following raw materials in parts by weight:
[0128] 6.5 parts by weight of rattan tea extract, 3.5 parts by weight of fine algae polysaccharide, 5.5 parts by weight of hydrolyzed collagen, and 2.5 parts by weight of acetylhydroxyproline. The rattan tea extract is the same as that in Example 1.
[0129] Embodiment 15
[0130] A composition with firming and anti-aging effects, comprising the following raw materials in parts by weight:
[0131] 3.5 parts by weight of fine algae polysaccharide, 5.5 parts by weight of hydrolyzed collagen, 2.5 parts by weight of acetylhydroxyproline, and 2 parts by weight of sodium polyaspartate.
[0132] Test Example 1
[0133] The superoxide anion radical scavenging rate of the composition described in the above example was determined by the NBT method. A phosphate buffer solution with a molar concentration of 0.05 M and a pH of 7.8 was used to prepare NBT solution (molar concentration of 2.25 mM), riboflavin solution (molar concentration of 60 μM), EDTA-Na 2 Solution (molar concentration is 3mM) and methionine solution (molar concentration is 14.5mM). Take 162mL of methionine solution, 162mL of EDTA-Na 2 6 mL of solution, 6 mL of NBT solution, and 6 mL of riboflavin solution were mixed evenly to obtain a reaction base solution (riboflavin solution was replaced with phosphate buffer as a zeroing base solution to eliminate the influence of the sample itself on the absorbance). 0.05 g of the composition sample was added to 2 mL of the reaction base solution (or zeroing base solution) and mixed evenly, then placed in an illumination box (4000 lux, 25°C) for 20 minutes, and then taken out to measure the absorbance OD at 560 nm. 1 (zero adjustment); then replace the composite sample with phosphate buffer as a blank group and repeat the above process to measure OD 0 The composition described in the above example was stored at room temperature in a light-proof environment for 12 months, and then the superoxide anion radical scavenging rate of the composition after 12 months of storage was measured again according to the above method, and the free radical scavenging rate change rate after 12 months of storage was calculated based on the free radical scavenging rates before and after storage.
[0134] Where, superoxide anion free radical scavenging rate % = (OD 0 -OD 1 ) / OD 0 ×100%; free radical scavenging rate change rate %=(free radical scavenging rate after storage-free radical scavenging rate before storage) / free radical scavenging rate before storage×100%. The test results are shown in Table 1.
[0135] Table 1. Antioxidant performance and stability test results
[0136]
[0137]
[0138] Test Example 2
[0139] 6 parts by weight of 1,3-propylene glycol, 8 parts by weight of glycerol, 1.5 parts by weight of ethylhexyl palmitate, 1.5 parts by weight of xanthan gum, 0.5 parts by weight of ethylhexyl glycerol, 7.5 parts by weight of the composition described in the above embodiment, and 100 parts by weight of water were mixed evenly to obtain test cosmetics. 150 female subjects aged between 30 and 50 were invited and randomly divided into 15 groups, 10 in each group. The subjects were in good health, had no skin diseases, and had non-sensitive skin. The subjects used the above cosmetics once in the morning and evening every day, and the test lasted for 1 month. No other cosmetics were used during the test, and long-term exposure to sunlight and ultraviolet rays was avoided to maintain a regular life. A skin detector was used to detect the improvement of the subjects' skin wrinkles ('-' means reduction) and skin elasticity ('+' means increase), and the firming and anti-aging effects of the composition were evaluated. The test results are shown in Table 2.
[0140] Table 2. Results of firming and anti-aging efficacy tests
[0141]
[0142]
[0143] It can be seen from the test results in Tables 1 and 2 that the compositions described in Examples 1-3 of the present invention have the best firming and anti-aging effects, and also have the highest antioxidant activity and stability. Compared with Examples 1-3, the compositions described in Examples 11-15 lack one of the five raw materials of rattan tea extract, fine algae polysaccharide, hydrolyzed collagen, acetylhydroxyproline, and sodium polyaspartate, respectively, resulting in relatively poor anti-aging efficacy and stability. This is because the compositions described in Examples 1-3 use a specific rattan tea extract and synergistic effects with fine algae polysaccharide, hydrolyzed collagen, acetylhydroxyproline and sodium polyaspartate, which can comprehensively improve the firmness and elasticity of the skin from multiple dimensions, reduce wrinkles, and achieve the purpose of anti-aging and skin care, while having the advantage of stable activity; Compared with Examples 1-3, the compositions described in Examples 4-10 do not use rattan tea extract prepared by a specific method, resulting in relatively poor antioxidant activity and anti-aging efficacy. This is because the compositions described in Examples 1-3 use a specific rattan tea extract, and by improving the preparation method of the rattan tea extract, the biological activity of the rattan tea extract is further improved from pretreatment, enzymatic hydrolysis, fermentation to supercritical extraction and other steps. This shows that since Example 4-15 did not adopt the above-mentioned necessary technical means, it showed a trend of deterioration in the corresponding performance tests, which proves the importance of the above-mentioned necessary technical means to improve its performance.
[0144] The preferred specific embodiments of the present invention are described in detail above. It should be understood that a person skilled in the art can make many modifications and changes based on the concept of the present invention without creative work. Therefore, any technical solution that can be obtained by a person skilled in the art through logical analysis, reasoning or limited experiments based on the concept of the present invention on the basis of the prior art should be within the scope of protection determined by the claims.
Claims
1. A composition with firming and anti-aging effects, characterized in that: It includes the following raw materials in parts by weight: 1-20 parts of rattan tea extract, 0.1-10 parts of fine algae polysaccharide, 1-15 parts of hydrolyzed collagen, 0.1-10 parts of acetylhydroxyproline, and 0.1-10 parts of sodium polyaspartate.
2. The composition with firming and anti-aging effects according to claim 1, characterized in that: It is composed of the following raw materials in parts by weight: 5-8 parts of rattan tea extract, 1-5 parts of fine algae polysaccharide, 4-7 parts of hydrolyzed collagen, 1-4 parts of acetylhydroxyproline, and 1-3 parts of sodium polyaspartate.
3. The composition with firming and anti-aging effects according to claim 1 or 2, characterized in that: The rattan tea extract is prepared by the following method: The rattan tea is crushed to obtain rattan tea powder; the rattan tea powder and regulating aid are added into water for ultrasonic treatment, and then a mixed enzyme is added for enzymolysis to obtain an enzymolysis product; fermentation bacteria are inoculated into the enzymolysis product for fermentation to obtain a fermentation product; the fermentation product is filtered, the filtrate is concentrated to obtain an extract I, and the filter residue is reserved; the filter residue is subjected to supercritical extraction, the extract is concentrated to obtain an extract II; the extract I is combined with the extract II to obtain a rattan tea extract.
4. The composition with firming and anti-aging effects according to claim 1 or 2, characterized in that: The rattan tea extract is prepared by the following method: The rattan tea is crushed to obtain rattan tea powder; 20-30 parts by weight of rattan tea powder and 3-7 parts by weight of a regulating aid are added to 250-350 parts by weight of water for ultrasonic treatment, and then 1.5-3 parts by weight of a mixed enzyme is added for enzymolysis, sterilized, and cooled to obtain an enzymolysis product; fermentation bacteria are inoculated into the enzymolysis product for fermentation, sterilized, and cooled to obtain a fermentation product; the fermentation product is filtered, the filtrate is collected and concentrated to 8-12% of the original volume to obtain an extract I, and the filter residue is reserved; the filter residue is placed in an extraction kettle, an entrainer is added, supercritical extraction is performed, the extract is collected, and the extract is concentrated to 8-12% of the original volume to obtain an extract II; the extract I and the extract II are combined to obtain a rattan tea extract.
5. The composition with firming and anti-aging effects according to claim 3 or 4, characterized in that: The regulating auxiliary agent is a mixture of D-sorbitol and Tween-20.
6. The composition with firming and anti-aging effects according to claim 3 or 4, characterized in that: The fermentation bacteria are Bacillus plantarum and / or Bacillus amyloliquefaciens.
7. The composition with firming and anti-aging effects according to claim 6, characterized in that: The fermentation bacteria consist of Bacillus plantarum and Bacillus amyloliquefaciens.
8. The composition with firming and anti-aging effects according to claim 3 or 4, characterized in that: The mixed enzyme is a mixture of cellulase, pectinase and papain.
9. The composition with firming and anti-aging effects according to claim 3 or 4, characterized in that: The entrainer is a mixture of anhydrous ethanol and water.
10. Use of the composition with firming and anti-aging effects according to any one of claims 1 to 9 in the preparation of cosmetics.
Citation Information
Patent Citations
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CN105055188A
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