Ginseng and radix paeoniae alba acne-removing composition and application thereof

Abstract

The invention discloses a ginseng and radix paeoniae alba acne-removing composition and application thereof. The invention relates to a ginseng and radix paeoniae alba acne removal composition, which is prepared from a radix sophorae flavescentis (SOPHORA FLASENS) root extract, a radix paeoniae rubra (PAEONIA VEITCHII) extract, an albizia julibrissin (ALBIZIA JUTIBRISSIN) bark extract and a fructus forsythiae (FORSYTHIA SUSPENSA) extract. The plant-extracted acne-removing composition is extracted and refined by adopting modern plant extraction technologies such as soaking, enzymolysis, alcohol extraction, column chromatography and centrifugation, and tests show that the plant-extracted acne-removing composition can effectively inhibit propagation of propionibacterium acnes, regulate health balance of skin flora, reduce lipid secretion of sebaceous gland cells, regulate water-fat balance of skin surfaces and prevent acne-removing effects. The generation of skin inflammatory factors is inhibited, and the skin is relieved and conditioned. The acne-removing composition is applied to cosmetics to achieve a good acne-removing effect.

Description

Technical Field

[0001] The invention relates to the field of cosmetics, and in particular to a ginseng and peony root acne-removing composition and application thereof. Background Art

[0002] Acne is the most common chronic inflammatory disease of the hair follicles and sebaceous glands in dermatology. In contemporary society, people pay more and more attention to skin management, and the use of certain cosmetics, contact with irritating biochemical reagents, irregular daily routines and bad eating habits are likely to cause acne. According to the 2022 statistics of the "Chinese Journal of Dermatology and Venereology", the incidence of acne in China has risen sharply, and it is common and the population is gradually expanding. More than 95% of people will have acne to varying degrees, and 3-7% of acne patients will have scars.

[0003] There are many types of acne removal products on the market, and some of the products with significant effects are irritating to a certain extent, and long-term use may even cause skin damage. For example, although glucocorticoid acne treatment drugs bring a short-term acne removal experience, long-term use will accumulate hormones on the skin, aggravate the sensitivity of the skin, and the skin will develop tolerance to such drugs, leading to hormone face. It is also necessary to use drugs according to doctor's advice, such as antibiotics such as metronidazole and chloramphenicol that have been banned in cosmetics. The application of retinol in cosmetic formulas has limitations, such as easy oxidation in the air and insoluble in water, which makes retinol's excellent acne removal effect unable to be fully exerted. Retinoic acid is also a common drug for treating acne. Its main ingredient is all-trans retinoic acid, which is easily decomposed by light. Its side effects can cause local skin irritation reactions such as erythema, desquamation, itching and stinging, etc., which is not suitable for sensitive skin.

[0004] The safety and efficacy of skin care products have always been the research focus of the cosmetics industry. In modern society, the number of people with sensitive skin has increased dramatically, and products that gently remove acne and have preventive effects have attracted more attention.

[0005] By adding active ingredients with antibacterial, anti-inflammatory and oil-control effects developed from natural plants, combined with the moisturizing and nutritional effects of the skin care products themselves, it has a good effect on mild acne removal. However, many acne removal products that claim to have added plant extracts are still at the publicity level, and no research has been conducted on the acne removal mechanism of the plant extracts added, nor on the acne removal effect actually achieved. For example, patent application CN202210327875.7 discloses a plant acne removal composition containing Sophora flavescens and Salvia miltiorrhiza and a preparation method thereof, claiming that it has excellent physical and chemical stability, excellent heat and cold resistance, and the prepared acne removal composition is easily soluble in water and easily absorbed by the skin. Its active ingredients act on the skin to effectively soothe the skin, inhibit skin allergies, enhance skin resistance, regulate skin metabolism, inhibit skin sebum secretion, and inhibit skin acne. However, the embodiment of its specification only verifies the stability, does not disclose any data on the efficacy mechanism, and does not verify the acne removal efficacy.

[0006] After extensive research in the field of acne treatment, the inventors of the present invention found that a plant extract acne treatment composition was obtained by using modern plant extraction technology to extract Sophora flavescens, Paeonia lactiflora, Albizzia julibrissin, and Fructus Forsythiae. Tests found that the plant extract acne treatment composition can effectively inhibit the reproduction of Propionibacterium acnes, regulate the healthy balance of skin flora, reduce the lipid secretion of sebaceous gland cells, regulate the water-fat balance of the skin surface, inhibit the production of skin inflammatory factors, and soothe and condition the skin. The application of the plant extract in cosmetics achieves a good acne treatment effect, thereby completing the present invention. Summary of the invention

[0007] In view of this, the object of the present invention is to provide a ginseng and peony root acne-removing composition.

[0008] The ginseng and peony acne-removing composition contains a root extract of Sophora flavescens, an extract of Paeonia veitchii, an extract of Albizia julibrissin bark, and an extract of Forsythia suspensa.

[0009] The sophora flavescens involved in the present invention is the dried root of the leguminous plant sophora flavescens, which has the functions of clearing away heat and dampness, killing insects and promoting diuresis. The Southern Yunnan Materia Medica records that it "cools blood, relieves heat and toxins, treats scabies, and pus nests and sores. It can treat skin itching, blood-wind ringworm sores, naughty white scales, intestinal wind bleeding, and blood in the stool. It can eliminate wind, swelling and toxins, and phlegm toxins". Sophora flavescens contains a variety of alkaloids, such as matrine, oxymatrine, isomatrine, sophoracarpine, isosophoracarpine, oxysophocarpine, sophoramine, etc., as well as flavonoid compounds such as matrine, neomatrine, matrine, isomatrine, and sophoramine.

[0010] The Sichuan red peony root involved in the present invention is the dried root of the Ranunculaceae plant Sichuan red peony root, which is bitter, slightly cold, and enters the liver meridian, and has the functions of clearing heat, cooling blood, and dispersing blood stasis. The main components are: paeoniflorin, paeoniflorin lactone glycosides, hydroxypeoniflorin, and benzoylpeoniflorin.

[0011] The Albizzia julibrissin involved in the present invention is the dried bark of a deciduous tree of the genus Albizzia in the Leguminosae family, which has the effects of relieving depression and calming the mind, promoting blood circulation and reducing swelling. The bark of the Albizzia julibrissin contains a variety of active ingredients, mainly including triterpenoid compounds, flavonoid compounds, alkaloids, polysaccharides, etc. Among them, the saponin compounds in the bark of the Albizzia julibrissin are its main effective ingredients and have significant pharmacological effects.

[0012] The forsythia suspensa involved in the present invention is a mature fruit of a shrub of the genus Forsythia of the family Oleaceae, which has the effects of clearing away heat and detoxifying, reducing swelling and dispersing nodules. Forsythia suspensa is rich in various biologically active ingredients, such as forsythiaside A, ethyl forsythia ethyl acetate, forsythiaside, forsythiaside and rutin.

[0013] Another object of the present invention is to provide a use for preparing a ginseng and peony root anti-acne composition.

[0014] In order to achieve the above-mentioned object of the invention, the present invention provides a composition for preparing a cosmetic composition, comprising a ginseng and peony acne-removing composition as an effective ingredient, and is used for removing acne.

[0015] The "acne removal" mentioned in the present invention refers to helping to reduce or slow down the occurrence of acne (including blackheads or whiteheads) and helping the skin recover after acne occurs.

[0016] The present invention provides the following technical solutions: A ginseng and peony acne-removing composition, characterized in that it comprises the following components: Sophora flavescens root extract, Paeonia veitchii extract, Albizia julibrissin bark extract, and Forsythia suspensa extract.

[0017] The embodiment of the present invention also provides a method for preparing the above-mentioned Shenshao anti-acne composition, comprising the following preparation steps: Step (1): weigh the following materials in parts by weight: 1-10 parts of Sophora flavescens, 1-10 parts of Paeonia lactiflora, 1-10 parts of Albizzia julibrissin, and 1-10 parts of Fructus Forsythiae, and grind them through a 30-50 mesh sieve to obtain Chinese medicine powder; add water and soak for 12-24 hours; Step (2): adjusting the pH to 7-8, adding cellulase and α-amylase, stirring and enzymolysis at 35-40°C for 3-5 hours; inactivating the enzyme with microwave to obtain an enzymolysis product; Step (3): adding ethanol to the hydrolysate, stirring and extracting for 3-5 hours, and centrifuging to obtain the supernatant; Step (4): concentrate the supernatant under reduced pressure and adjust the pH to 8-9; apply it to a D101 macroporous resin column at a flow rate of 1-2 BV / h; then wash 3-5 BV with water, and then elute 3-5 BV with 50-70% V / V ethanol, and collect the ethanol eluate; Step (5): adding 1,3-butanediol to the ethanol eluate, removing the ethanol by rotary evaporation, and collecting the supernatant by centrifugation to obtain the ginseng and peony root anti-acne composition.

[0018] In some embodiments, the mass ratio of the Chinese medicine powder and water in step (1) is 1:10-20.

[0019] In some embodiments, the cellulase described in step (2) is a food-grade enzyme preparation, with an enzyme activity of >100,000 U / g, and the amount added is 1% of the weight of the Chinese medicine powder; the α-amylase described is a food-grade enzyme preparation, with an enzyme activity of >2000 U / g, and the amount added is 2% of the weight of the Chinese medicine powder. The cell wall of natural plants is composed of cellulose, and its active ingredients are wrapped in the cell wall. The enzymatic method is used to destroy the cell wall of the plant to facilitate the maximum dissolution of the active ingredients, thereby greatly improving the extraction rate of the active ingredients in the natural product and further promoting the rational use of the beneficial ingredients in the plant.

[0020] In some embodiments, the microwave enzyme inactivation condition in step (2) is microwave treatment at 70-80°C for 1-3 minutes. The microwave enzyme inactivation equipment uses the thermal effect and non-thermal effect of microwaves to inactivate the enzymes in the material. Microwaves can quickly penetrate the interior of the material, causing the water molecules in the material to generate friction heat, thereby achieving the purpose of enzyme inactivation. It has the advantages of high efficiency, uniform heating, avoidance of local overheating, and short heating time, thereby minimizing the loss of active ingredients in the material; in addition, no additional heat conduction medium is required during the heating process, reducing energy consumption and environmental pollution.

[0021] In some embodiments, the ethanol in step (3) is anhydrous ethanol, and the mass ratio of the enzymatic hydrolysate to the ethanol is 1:0.5-1.

[0022] In some embodiments, the supernatant in step (4) is concentrated under reduced pressure to a solid content of 0.2-0.5 g / mL.

[0023] In some of the embodiments, the amount of 1,3-butanediol added in step (5) is 10-30% by weight of the ethanol eluent.

[0024] The ginseng and peony acne-removing composition prepared in the embodiment of the present invention can be used as an acne-removing active ingredient in a cosmetic formula, and its addition amount can be 1-10wt%. The cosmetic can be in the form of cream, lotion, facial mask, essence, facial cleanser, etc.

[0025] The embodiment of the present invention also discloses an acne-removing essence, which contains the following components in percentage by weight: 5% butylene glycol, 0.06% xanthan gum, 0.5% sodium polyacryloyldimethyltaurate, 0.7% ammonium acryloyldimethyltaurate / behenyl alcohol polyether-25 methacrylate cross-linked polymer, 0.15% allantoin, 0.3% tetrahydromethylpyrimidine carboxylic acid, 8% ethoxydiglycol, 2% diethoxyethyl succinate, 0.1% lactobionic acid, 1-10% of the above-mentioned ginseng and peony acne-removing composition, and the balance water.

[0026] The technical solution provided by the embodiment of the present invention has the following beneficial effects: Sophora flavescens, Paeonia lactiflora, Albizzia julibrissin, and Fructus Forsythiae are selected, and a plant extract anti-acne composition is obtained by extracting and refining using modern plant extraction techniques such as immersion, enzymolysis, alcohol extraction, column chromatography, and centrifugation. Tests have found that the plant extract anti-acne composition can effectively inhibit the reproduction of Propionibacterium acnes, regulate the healthy balance of skin flora, reduce the lipid secretion of sebaceous gland cells, regulate the water-fat balance of the skin surface, inhibit the production of skin inflammatory factors, and soothe and condition the skin. The application of the plant extract in cosmetics achieves a good anti-acne effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for describing the embodiments or the prior art are briefly introduced below.

[0028] Figure 1 The diameters of the inhibition zones of each test group in Experiment 1 are shown. DETAILED DESCRIPTION

[0029] The embodiments in the specification are only used to illustrate the present invention and do not limit the scope of protection of the present invention. The scope of protection of the present invention is only limited by the claims, and any omission, replacement or modification made on the basis of the embodiments disclosed in the present invention shall fall within the scope of protection of the present invention.

[0030] The content of the present invention can be more easily understood by selecting the following detailed description of the preferred implementation method of the present invention and the embodiments included. Unless otherwise specified, all technical and scientific terms used herein have the same meanings as those of ordinary skill in the art to which the present invention belongs. When there is a conflict, the definition in this specification shall prevail.

[0031] As used herein, the term "prepared from" is synonymous with "comprising." As used herein, the terms "comprising," "including," "having," "containing," or any other variation thereof, are intended to cover a non-exclusive inclusion. For example, a composition, process, method, article, or apparatus that comprises the listed elements is not necessarily limited to only those elements but may include other elements not expressly listed or inherent to such composition, process, method, article, or apparatus.

[0032] When amount, concentration or other value or parameter is expressed as range, preferred range or a series of upper preferred value and lower preferred value limit range, this should be understood as specifically disclosing all ranges formed by any pairing of any range upper limit or preferred value and any range lower limit or preferred value, regardless of whether the range is disclosed separately. For example, when disclosing range "1 to 5", described range should be interpreted as including range "1 to 4", "1 to 3", "1 to 2", "1 to 2 and 4 to 5", "1 to 3 and 5" etc. When numerical range is described in this article, unless otherwise stated, the range is intended to include its end value and all integers and fractions within the range.

[0033] In addition, the indefinite articles "a" and "an" before the elements or components of the present invention have no restrictions on the quantity requirements (i.e. the number of occurrences) of the elements or components. Therefore, "a" or "an" should be interpreted as including one or at least one, and the elements or components in the singular form also include the plural form, unless the number is obviously intended to be in the singular form.

[0034] In order to deepen the understanding of the present invention, the present invention will be further described in detail below in conjunction with examples. The examples are only used to explain the present invention and do not constitute a limitation on the protection scope of the present invention. Example

[0035] A method for preparing the above-mentioned ginseng and peony acne-removing composition comprises the following preparation steps: Step (1): Weigh the following materials in parts by weight: 1 part of Sophora flavescens, 10 parts of Paeonia lactiflora, 1 part of Albizzia julibrissin, and 10 parts of Fructus Forsythiae, and grind them through a 30-mesh sieve to obtain Chinese medicine powder; add water at a material-liquid ratio of 1:20, and soak for 12 hours; Step (2): adjusting the pH to 8, adding cellulase and α-amylase, wherein the cellulase is a food-grade enzyme preparation with an enzyme activity of >100,000 U / g, and the added amount is 1% of the weight of the Chinese medicine powder; the α-amylase is a food-grade enzyme preparation with an enzyme activity of >2000 U / g, and the added amount is 2% of the weight of the Chinese medicine powder; stirring and enzymolyzing at 35°C for 5 hours; microwave treatment at 70°C for 3 minutes to inactivate the enzyme, and obtaining an enzymolyzate; Step (3): adding anhydrous ethanol to the hydrolysate, wherein the mass ratio of the hydrolysate to the anhydrous ethanol is 1:0.5; stirring and extracting for 5 hours, and centrifuging to obtain the supernatant; Step (4): concentrate the supernatant under reduced pressure to a solid content of 0.2 g / mL, adjust the pH to 9; apply the supernatant to a D101 macroporous resin column at a flow rate of 1 BV / h; then wash 5 BV with water, then elute 5 BV with 50% V / V ethanol, and collect the ethanol eluate; Step (5): adding 1,3-butanediol to the ethanol eluate, wherein the amount of 1,3-butanediol added is 10% of the weight of the ethanol eluate; removing the ethanol by rotary evaporation, and collecting the supernatant by centrifugation to obtain the ginseng and peony root anti-acne composition. Example

[0036] A method for preparing the above-mentioned ginseng and peony acne-removing composition comprises the following preparation steps: Step (1): Weigh the following materials in parts by weight: 10 parts of Sophora flavescens, 1 part of Paeonia lactiflora, 10 parts of Albizzia julibrissin, and 1 part of Fructus Forsythiae, and grind them through a 50-mesh sieve to obtain Chinese medicine powder; add water at a material-liquid ratio of 1:10, and soak for 24 hours; Step (2): adjusting the pH to 7, adding cellulase and α-amylase, wherein the cellulase is a food-grade enzyme preparation with an enzyme activity of >100,000 U / g, and the added amount is 1% of the weight of the Chinese medicine powder; the α-amylase is a food-grade enzyme preparation with an enzyme activity of >2000 U / g, and the added amount is 2% of the weight of the Chinese medicine powder; stirring and enzymolyzing at 40°C for 3 hours; microwave treatment at 80°C for 1 minute to inactivate the enzyme, and obtaining an enzymolyzate; Step (3): adding anhydrous ethanol to the hydrolysate, wherein the mass ratio of the hydrolysate to the anhydrous ethanol is 1:1; stirring and extracting for 3 hours, and centrifuging to obtain a supernatant; Step (4): concentrate the supernatant under reduced pressure to a solid content of 0.5 g / mL, adjust the pH to 8; apply the supernatant to a D101 macroporous resin column at a flow rate of 2 BV / h; then wash 3 BV with water, and then elute 3 BV with 70% V / V ethanol, and collect the ethanol eluate; Step (5): adding 1,3-butanediol to the ethanol eluate, wherein the amount of 1,3-butanediol added is 30% of the weight of the ethanol eluate; removing the ethanol by rotary evaporation, and collecting the supernatant by centrifugation to obtain the ginseng and peony root anti-acne composition. Example

[0037] A method for preparing the above-mentioned ginseng and peony acne-removing composition comprises the following preparation steps: Step (1): weigh the following materials in parts by weight: 8 parts of Sophora flavescens, 3 parts of Paeonia lactiflora, 7 parts of Albizzia julibrissin, and 5 parts of Fructus Forsythiae, grind them through a 40-mesh sieve to obtain Chinese medicine powder; add water at a material-liquid ratio of 1:15, and soak for 18 hours; Step (2): adjusting the pH to 7.5, adding cellulase and α-amylase, wherein the cellulase is a food-grade enzyme preparation with an enzyme activity of >100,000 U / g, and the added amount is 1% of the weight of the Chinese medicine powder; the α-amylase is a food-grade enzyme preparation with an enzyme activity of >2000 U / g, and the added amount is 2% of the weight of the Chinese medicine powder; stirring and enzymolyzing at 38°C for 4 hours; microwave treatment at 75°C for 2 minutes to inactivate the enzyme, and obtaining an enzymolyzate; Step (3): adding anhydrous ethanol to the hydrolysate, wherein the mass ratio of the hydrolysate to the anhydrous ethanol is 1:0.8; stirring and extracting for 4 hours, and centrifuging to obtain the supernatant; Step (4): concentrate the supernatant under reduced pressure to a solid content of 0.3 g / mL, adjust the pH to 8.5; apply the supernatant to a D101 macroporous resin column at a flow rate of 1.5 BV / h; then wash with water for 4 BV, then elute with 60% V / V ethanol for 4 BV, and collect the ethanol eluate; Step (5): adding 1,3-butanediol to the ethanol eluate, wherein the amount of 1,3-butanediol added is 20% of the weight of the ethanol eluate; removing the ethanol by rotary evaporation, and collecting the supernatant by centrifugation to obtain the ginseng and peony root acne-removing composition.

[0038] In order to investigate the relationship between Sophora flavescens, Paeonia lactiflora, Albizzia julibrissin, and Forsythia suspensa powder in the ginseng and peony acne-removing composition provided in the embodiments of the present invention, comparative examples 1-4 are specially set.

[0039] Comparative Example 1 A method for preparing a ginseng and peony root acne-removing composition, comprising the following preparation steps: Step (1): crush Sophora flavescens through a 40-mesh sieve to obtain Chinese medicine powder; add water at a solid-liquid ratio of 1:15, and soak for 18 hours; Step (2): adjusting the pH to 7.5, adding cellulase and α-amylase, wherein the cellulase is a food-grade enzyme preparation with an enzyme activity of >100,000 U / g, and the added amount is 1% of the weight of the Chinese medicine powder; the α-amylase is a food-grade enzyme preparation with an enzyme activity of >2000 U / g, and the added amount is 2% of the weight of the Chinese medicine powder; stirring and enzymolyzing at 38°C for 4 hours; microwave treatment at 75°C for 2 minutes to inactivate the enzyme, and obtaining an enzymolyzate; Step (3): adding anhydrous ethanol to the hydrolysate, wherein the mass ratio of the hydrolysate to the anhydrous ethanol is 1:0.8; stirring and extracting for 4 hours, and centrifuging to obtain the supernatant; Step (4): concentrate the supernatant under reduced pressure to a solid content of 0.3 g / mL, adjust the pH to 8.5; apply the supernatant to a D101 macroporous resin column at a flow rate of 1.5 BV / h; then wash with water for 4 BV, then elute with 60% V / V ethanol for 4 BV, and collect the ethanol eluate; Step (5): adding 1,3-butanediol to the ethanol eluate, wherein the amount of 1,3-butanediol added is 20% of the weight of the ethanol eluate; removing the ethanol by rotary evaporation, and collecting the supernatant by centrifugation to obtain the ginseng and peony root acne-removing composition.

[0040] Comparative Example 2 A method for preparing the above-mentioned ginseng and peony acne-removing composition comprises the following preparation steps: Step (1): crush the red peony root through a 40-mesh sieve to obtain a Chinese medicine powder; add water at a solid-liquid ratio of 1:15, and soak for 18 hours; Step (2): adjusting the pH to 7.5, adding cellulase and α-amylase, wherein the cellulase is a food-grade enzyme preparation with an enzyme activity of >100,000 U / g, and the added amount is 1% of the weight of the Chinese medicine powder; the α-amylase is a food-grade enzyme preparation with an enzyme activity of >2000 U / g, and the added amount is 2% of the weight of the Chinese medicine powder; stirring and enzymolyzing at 38°C for 4 hours; microwave treatment at 75°C for 2 minutes to inactivate the enzyme, and obtaining an enzymolyzate; Step (3): adding anhydrous ethanol to the hydrolysate, wherein the mass ratio of the hydrolysate to the anhydrous ethanol is 1:0.8; stirring and extracting for 4 hours, and centrifuging to obtain the supernatant; Step (4): concentrate the supernatant under reduced pressure to a solid content of 0.3 g / mL, adjust the pH to 8.5; apply the supernatant to a D101 macroporous resin column at a flow rate of 1.5 BV / h; then wash with water for 4 BV, then elute with 60% V / V ethanol for 4 BV, and collect the ethanol eluate; Step (5): adding 1,3-butanediol to the ethanol eluate, wherein the amount of 1,3-butanediol added is 20% of the weight of the ethanol eluate; removing the ethanol by rotary evaporation, and collecting the supernatant by centrifugation to obtain the ginseng and peony root acne-removing composition.

[0041] Comparative Example 3 A method for preparing the above-mentioned ginseng and peony acne-removing composition comprises the following preparation steps: Step (1): crush the Albizzia Julibrissin through a 40-mesh sieve to obtain a Chinese medicine powder; add water at a solid-liquid ratio of 1:15, and soak for 18 hours; Step (2): adjusting the pH to 7.5, adding cellulase and α-amylase, wherein the cellulase is a food-grade enzyme preparation with an enzyme activity of >100,000 U / g, and the added amount is 1% of the weight of the Chinese medicine powder; the α-amylase is a food-grade enzyme preparation with an enzyme activity of >2000 U / g, and the added amount is 2% of the weight of the Chinese medicine powder; stirring and enzymolyzing at 38°C for 4 hours; microwave treatment at 75°C for 2 minutes to inactivate the enzyme, and obtaining an enzymolyzate; Step (3): adding anhydrous ethanol to the hydrolysate, wherein the mass ratio of the hydrolysate to the anhydrous ethanol is 1:0.8; stirring and extracting for 4 hours, and centrifuging to obtain the supernatant; Step (4): concentrate the supernatant under reduced pressure to a solid content of 0.3 g / mL, adjust the pH to 8.5; apply the supernatant to a D101 macroporous resin column at a flow rate of 1.5 BV / h; then wash with water for 4 BV, then elute with 60% V / V ethanol for 4 BV, and collect the ethanol eluate; Step (5): adding 1,3-butanediol to the ethanol eluate, wherein the amount of 1,3-butanediol added is 20% of the weight of the ethanol eluate; removing the ethanol by rotary evaporation, and collecting the supernatant by centrifugation to obtain the ginseng and peony root acne-removing composition.

[0042] Comparative Example 4 A method for preparing the above-mentioned ginseng and peony acne-removing composition comprises the following preparation steps: Step (1): crush the Forsythia suspensa through a 40-mesh sieve to obtain a Chinese medicine powder; add water at a solid-liquid ratio of 1:15, and soak for 18 hours; Step (2): adjusting the pH to 7.5, adding cellulase and α-amylase, wherein the cellulase is a food-grade enzyme preparation with an enzyme activity of >100,000 U / g, and the added amount is 1% of the weight of the Chinese medicine powder; the α-amylase is a food-grade enzyme preparation with an enzyme activity of >2000 U / g, and the added amount is 2% of the weight of the Chinese medicine powder; stirring and enzymolyzing at 38°C for 4 hours; microwave treatment at 75°C for 2 minutes to inactivate the enzyme, and obtaining an enzymolyzate; Step (3): adding anhydrous ethanol to the hydrolysate, wherein the mass ratio of the hydrolysate to the anhydrous ethanol is 1:0.8; stirring and extracting for 4 hours, and centrifuging to obtain the supernatant; Step (4): concentrate the supernatant under reduced pressure to a solid content of 0.3 g / mL, adjust the pH to 8.5; apply the supernatant to a D101 macroporous resin column at a flow rate of 1.5 BV / h; then wash with water for 4 BV, then elute with 60% V / V ethanol for 4 BV, and collect the ethanol eluate; Step (5): adding 1,3-butanediol to the ethanol eluate, wherein the amount of 1,3-butanediol added is 20% of the weight of the ethanol eluate; removing the ethanol by rotary evaporation, and collecting the supernatant by centrifugation to obtain the ginseng and peony root acne-removing composition.

[0043] In order to investigate the effect of the combined enzymatic hydrolysis of cellulase and α-amylase on the efficacy of the product in the preparation method of the ginseng and peony acne-removing composition provided in the embodiment of the present invention, comparative examples 5-6 are specially set.

[0044] Comparative Example 5 A method for preparing the above-mentioned ginseng and peony acne-removing composition comprises the following preparation steps: Step (1): weigh the following materials in parts by weight: 8 parts of Sophora flavescens, 3 parts of Paeonia lactiflora, 7 parts of Albizzia julibrissin, and 5 parts of Fructus Forsythiae, grind them through a 40-mesh sieve to obtain Chinese medicine powder; add water at a material-liquid ratio of 1:15, and soak for 18 hours; Step (2): adjusting the pH to 7.5, adding cellulase, wherein the cellulase is a food-grade enzyme preparation, the enzyme activity is greater than 100,000 U / g, and the added amount is 1% of the weight of the Chinese medicine powder; stirring and enzymolysis at 38°C for 4 hours; microwave treatment at 75°C for 2 minutes to inactivate the enzyme, and obtain an enzymolysis product; Step (3): adding anhydrous ethanol to the hydrolysate, wherein the mass ratio of the hydrolysate to the anhydrous ethanol is 1:0.8; stirring and extracting for 4 hours, and centrifuging to obtain the supernatant; Step (4): concentrate the supernatant under reduced pressure to a solid content of 0.3 g / mL, adjust the pH to 8.5; apply the supernatant to a D101 macroporous resin column at a flow rate of 1.5 BV / h; then wash with water for 4 BV, then elute with 60% V / V ethanol for 4 BV, and collect the ethanol eluate; Step (5): adding 1,3-butanediol to the ethanol eluate, wherein the amount of 1,3-butanediol added is 20% of the weight of the ethanol eluate; removing the ethanol by rotary evaporation, and collecting the supernatant by centrifugation to obtain the ginseng and peony root acne-removing composition.

[0045] Comparative Example 6 A method for preparing the above-mentioned ginseng and peony acne-removing composition comprises the following preparation steps: Step (1): weigh the following materials in parts by weight: 8 parts of Sophora flavescens, 3 parts of Paeonia lactiflora, 7 parts of Albizzia julibrissin, and 5 parts of Fructus Forsythiae, grind them through a 40-mesh sieve to obtain Chinese medicine powder; add water at a material-liquid ratio of 1:15, and soak for 18 hours; Step (2): adjusting the pH to 7.5, adding α-amylase, wherein the α-amylase is a food-grade enzyme preparation, the enzyme activity is greater than 2000 U / g, and the addition amount is 2% of the weight of the traditional Chinese medicine powder; stirring the enzymolysis at 38°C for 4 hours; microwave treatment at 75°C for 2 minutes to inactivate the enzyme, and obtaining an enzymolysis product; Step (3): adding anhydrous ethanol to the hydrolysate, wherein the mass ratio of the hydrolysate to the anhydrous ethanol is 1:0.8; stirring and extracting for 4 hours, and centrifuging to obtain the supernatant; Step (4): concentrate the supernatant under reduced pressure to a solid content of 0.3 g / mL, adjust the pH to 8.5; apply the supernatant to a D101 macroporous resin column at a flow rate of 1.5 BV / h; then wash with water for 4 BV, then elute with 60% V / V ethanol for 4 BV, and collect the ethanol eluate; Step (5): adding 1,3-butanediol to the ethanol eluate, wherein the amount of 1,3-butanediol added is 20% of the weight of the ethanol eluate; removing the ethanol by rotary evaporation, and collecting the supernatant by centrifugation to obtain the ginseng and peony root acne-removing composition.

[0046] Test 1 Propionibacterium acnes inhibition test Experimental purpose: To evaluate the inhibitory effect of the ginseng and peony acne-removing composition prepared in the embodiment of the present invention on Propionibacterium acnes and verify its potential antibacterial activity.

[0047] 1.1 Sample preparation The ginseng and peony acne-removing compositions prepared in Examples 1-3 and Comparative Examples 1-6 were diluted 10 times with water, 5 sterilized filter papers with a diameter of 6 mm were placed in the mixture, and the mixture was soaked for 2 hours, and then dried naturally for later use; 1.2 Preparation of plates The solid culture medium was dissolved by microwave heating, cooled to 50-60°C, 15 mL was taken with a sterile pipette and added to a culture dish, cooled to a solid culture medium, 0.1 mL of the test bacterial suspension was taken with a sterile pipette and activated on a plate, and spread evenly with a spreader, wherein the test bacteria had a bacterial content of 10 5 -10 6 CFU / mL of Propionibacterium acnes.

[0048] 1.3 Inhibition zone test Each plate was divided into 4 small areas. Sterilized filter paper was pasted into the small areas of the plate. The plate with the filter paper was inverted and placed in a 37 ℃ incubator for 24 h. The diameter of the inhibition zone was measured. The measurement was repeated 3 times and the relevant data were recorded. Figure 1 .

[0049] From the attached Figure 1 It can be seen that the ginseng and peony acne-removing compositions prepared in Examples 1-3 of the present invention have stronger inhibitory effects on Propionibacterium acnes than those in Comparative Examples 1-6.

[0050] Experiment 2 Inflammatory factor inhibition test based on RAW264.7 macrophages This experiment was carried out according to the method described in the literature "Guo Haijiao, Wang Xiaona, Yang Suzhen, et al. Research on the skin care efficacy of zinc hyaluronate [J]. Surfactant Chemical Industry (Chinese and English), 2023, 53(12): 1429-1436."

[0051] Test solution: The ginseng and peony acne-removing compositions prepared in Examples 1-3 and Comparative Examples 1-6 were diluted 100 times with culture medium respectively.

[0052] RAW264.7 macrophages were cultured at 2.2×10 5 / well inoculated into a 96-well plate, add 2 mL of cell suspension to each well, and place the inoculated cell culture plate in an incubator for 24 h (5% CO 2 , 37℃). Aspirate and discard the old cell culture medium in the well. Add 1.8mL of the test solution to each well of the sample group. Add 1.8mL of the solvent control culture solution to each well of the negative control group; add 1.8mL of the positive control culture solution (100μg / mL dexamethasone solution) to each well of the positive control group; add 1.8mL of the normal culture solution to each well of the blank control group. After the administration, the 6-well plate was placed in a cell culture incubator for 2h. After 2h, 200μL of the working solution containing 200ng / mL LPS (lipopolysaccharide) was added to each well of each group and placed in a cell culture incubator for further culturing for 22h. Collect the cell culture supernatant and detect IL-1α and PGE2 according to the instructions of the ELISA kit. The specific test results are shown in Table 1.

[0053] Table 1 Inflammatory factor detection results

[0054] As can be seen from Table 1, the ability of the ginseng and peony acne-removing compositions prepared in Examples 1-3 of the present invention to inhibit inflammatory factors is better than that of Comparative Examples 1-6.

[0055] Example 4 An acne-removing essence contains the following components in percentage by weight: 5% butylene glycol, 0.06% xanthan gum, 0.5% sodium polyacryloyldimethyltaurate, 0.7% ammonium acryloyldimethyltaurate / behenylether-25 methacrylate crosspolymer, 0.15% allantoin, 0.3% tetrahydromethylpyrimidine carboxylic acid, 8% ethoxydiglycol, 2% diethoxyethyl succinate, 5% of the ginseng and peony acne-removing composition prepared in Example 3, and the balance water.

[0056] Experiment 4: Volunteer Trial Test 15 volunteers were recruited, both male and female, aged 18-30 years old, with oily skin, mild to moderate acne (acne or inflammatory papules and pustules) on the face, without pustules, cysts, or nodules. Acne number requirements: mild acne is not less than 10 acne, moderate acne plus facial papules and pustules is not more than 30. Volunteer to participate and sign the informed consent form, and have not undergone other clinical efficacy tests recently. After cleansing the face, the volunteers used the acne essence prepared in Example 4 of the present invention, applied an appropriate amount to the acne on the face, and gently massaged in circles for absorption. Once in the morning and evening, after 14 days of continuous use, the volunteers were visited again, and the self-perception evaluation data of the volunteers were counted. It was learned that 12 of the volunteers reported that the number of acne decreased and was less than 10, the inflammatory papules and pustules disappeared, and the skin oil production was significantly reduced; 3 volunteers reported that acne was reduced to a certain extent, inflammation and skin oil production were reduced, and the total effective rate reached 100%, and all volunteers reported that there was no irritation or allergy. It can be seen that the ginseng and peony acne-removing composition prepared in the embodiment of the present invention can play a good acne-removing effect when used in cosmetics.

[0057] The technical features of the above embodiments may be combined arbitrarily. To make the description concise, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0058] The above embodiments only express several implementation methods of the present invention, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of the invention patent. It should be pointed out that, for those of ordinary skill in the art, several variations and improvements can be made without departing from the concept of the present invention, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.

Claims

1. A ginseng and peony root anti-acne composition, characterized in that: It includes the following components: Sophora flavescens root extract, Paeonia veitchii extract, Albizia julibrissin bark extract, and Forsythia suspensa extract.

2. The ginseng and peony root anti-acne composition according to claim 1, characterized in that: The preparation method comprises the following steps: Step (1): weigh the following materials in parts by weight: 1-10 parts of Sophora flavescens, 1-10 parts of Paeonia lactiflora, 1-10 parts of Albizzia julibrissin, and 1-10 parts of Fructus Forsythiae, and grind them through a 30-50 mesh sieve to obtain Chinese medicine powder; add water and soak for 12-24 hours; Step (2): adjusting the pH to 7-8, adding cellulase and α-amylase, stirring and enzymolysis at 35-40°C for 3-5 hours; inactivating the enzyme with microwave to obtain an enzymolysis product; Step (3): adding ethanol to the hydrolysate, stirring and extracting for 3-5 hours, and centrifuging to obtain the supernatant; Step (4): concentrate the supernatant under reduced pressure and adjust the pH to 8-9; apply it to a D101 macroporous resin column at a flow rate of 1-2 BV / h; then wash 3-5 BV with water, and then elute 3-5 BV with 50-70% V / V ethanol, and collect the ethanol eluate; Step (5): adding 1,3-butanediol to the ethanol eluate, removing the ethanol by rotary evaporation, and collecting the supernatant by centrifugation to obtain the ginseng and peony root anti-acne composition.

3. The ginseng and peony root anti-acne composition according to claim 2, characterized in that: The mass ratio of the Chinese medicine powder and water in step (1) is 1:10-20.

4. The ginseng and peony root anti-acne composition according to claim 2, characterized in that: The enzymatic activity of the cellulase in step (2) is greater than 100,000 U / g, and the amount added is 1% of the weight of the Chinese medicine powder; the enzymatic activity of the α-amylase is greater than 2000 U / g, and the amount added is 2% of the weight of the Chinese medicine powder.

5. The ginseng and peony root anti-acne composition according to claim 2, characterized in that: The microwave enzyme inactivation conditions in step (2) are microwave treatment at 70-80° C. for 1-3 min.

6. The ginseng and peony root anti-acne composition according to claim 2, characterized in that: The ethanol in step (3) is anhydrous ethanol, and the mass ratio of the enzymatic hydrolysate to the ethanol is 1:0.5-1.

7. The acne-removing composition of claim 2, characterized in that: The supernatant in step (4) is concentrated under reduced pressure to a solid content of 0.2-0.5 g / mL.

8. The acne-removing composition of claim 2, characterized in that: The amount of 1,3-butanediol added in step (5) is 10-30% of the weight of the ethanol eluent.

9. Use of the ginseng and peony root anti-acne composition according to any one of claims 1 to 8 in the preparation of cosmetics.

10. An anti-acne essence, characterized in that: The invention comprises the following components in percentage by weight: 5% butylene glycol, 0.06% xanthan gum, 0.5% sodium polyacryloyldimethyltaurate, 0.7% ammonium acryloyldimethyltaurate / behenyl alcohol polyether-25 methacrylate cross-linked polymer, 0.15% allantoin, 0.3% tetrahydromethylpyrimidine carboxylic acid, 8% ethoxydiglycol, 2% diethoxyethyl succinate, 0.1% lactobionic acid, 1-10% of the ginseng and peony acne-removing composition according to any one of claims 1 to 8, and the balance water.

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