A yang-warming and depression-relieving traditional chinese medicine composition for treating parkinson's disease and application thereof

Abstract

The application belongs to the technical field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine composition for resisting Parkinson's disease and application thereof. The traditional Chinese medicine composition for resisting Parkinson's disease is made of raw materials in the following proportions by weight: raw aconite 5-15 parts, fried licorice 10-120 parts, dried ginger 5-50 parts, bupleurum 5-50 parts, peony 10-80 parts, bitter orange fruit 5-50 parts, and chuanxiong 2-12 parts. The application finds that the basic pathogenesis of Parkinson's disease is yang deficiency and liver depression, which plays a very important role in the occurrence, development and outcome of Parkinson's disease. Therefore, the traditional Chinese medicine composition provided by the application warms and supplements yang qi, soothes qi movement, supplements yang, unblocks qi, softens yin, and has the effects of warming yang and relieving depression, so as to improve the clinical symptoms of Parkinson's disease as a whole.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of traditional Chinese medicine, and particularly relates to a traditional Chinese medicine composition for resisting Parkinson's disease and application thereof. BACKGROUND

[0002] With the aggravation of population aging, it is predicted that the burden of Parkinson's disease (PD) will significantly increase in the next few decades, becoming the second most common neurodegenerative disease, and the prevalence rate is on the rise. Although modern medicine has made some progress in the treatment of Parkinson's disease, the overall course has not been significantly shortened, and the survival rate within ten years of the disease is about 50%. Parkinson's disease is generally divided into four types according to the clinical symptom type, namely, rigid type, hypokinesia type, tremor type, and type with or without dementia. The rigid type and the hypokinesia type account for about half of the total, and the overall performance shows characteristics such as limb stiffness, slow movement, shortness of breath, lazy speech, cold limbs, weak pulse, pale face, thin lips, thin stool, long urine, or mental depression, and thinking without sleep. In addition, it is different from other types of Parkinson's disease, with relatively fast development speed, relatively long course, and relatively early onset.

[0003] The motor symptoms of Parkinson's disease are similar to the "frightening" syndrome in traditional Chinese medicine, and the ancient books often use the words "frightening, falling, shaking, trembling, and convulsions" to describe its performance. In the Treatise on Febrile Diseases, it is described as "disease shaking", "dizziness and dizziness", and "shaking, falling, and drumming". The non-motor symptoms (NMS) of Parkinson's disease belong to the categories of "depression", "insomnia", and "constipation", including symptoms such as olfactory dysfunction, sleep disorders, constipation, depression, anxiety, and so on. Depression is a common non-motor disorder symptom of PD, which occurs in all stages of Parkinson's disease, and even before the appearance of motor symptoms. Patients with Parkinson's disease depression (PDD) show persistent low mood, difficulty concentrating, loss of interest in work and life, sleep disorders, apathy, pessimism, lack of humor, suicidal thoughts, anxiety, and sensitivity.

[0004] Studies have shown that traditional Chinese medicine has unique advantages in prolonging survival, improving quality of life, reducing adverse reactions, and reducing recurrence and metastasis. SUMMARY

[0005] The purpose of the present application is to provide a traditional Chinese medicine composition for resisting Parkinson's disease.

[0006] Another purpose of the present application is to provide the application of the above-mentioned traditional Chinese medicine composition.

[0007] According to clinical experience and theoretical research, in the basic pathogenesis of PDD, the deficiency of the spleen and kidney yang of the predestined and acquired yang leads to the inability of warming, promoting and driving, resulting in the lack of nourishment of the muscles, bones and tendons of the limbs, the lack of nourishment of the brain essence, the deficiency of the liver qi, the loss of control of the limbs and tremor, and the pathological manifestations of the spirit and emotion. The yang qi is closely related to the function of the viscera and the driving of the spirit and body. The spleen governs the muscles of the limbs, the liver governs the tendons and vessels, and the kidney governs the bones. The limbs are composed of bones, muscles and tendons. The filling of the three is the yin essence, but the yang qi is needed to transform and nourish. The use of the limbs relies on the active nature of the yang. The deficiency of the spleen and kidney yang and the stagnation of the liver qi interact with each other and jointly cause the motor and non-motor pathological symptoms of Parkinson's disease.

[0008] Therefore, the application provides a traditional Chinese medicine composition, which comprises raw aconite, fried licorice, dried ginger, bupleurum, paeony, citron fruit and chuanxiong, and aims at improving the clinical symptoms of Parkinson's disease from the aspects of warming and tonifying the yang qi and relieving the qi movement.

[0009] The application takes the basic pathogenesis of Parkinson's disease as the starting point, refers to the pathological research of Parkinson's disease, and proposes to treat Parkinson's disease from the aspects of warming and tonifying the spleen and kidney yang and relieving the liver qi movement, takes warming the yang, dispelling the cold and relieving the stagnation as the main treatment method, takes the four counterflow decoction combined with the four counterflow powder as the basic prescription, and uses the four counterflow decoction which can warm the yang as the main treatment method, so as to treat the symptoms and the root cause, and achieve the synergistic effect. Meanwhile, the application takes the commonness of human diseases as the starting point, and carries out animal experiments to prove the feasibility, effectiveness and scientificity of the application through deep basic research.

[0010] According to the anti-Parkinson's compound traditional Chinese medicine composition of the embodiment of the application, the traditional Chinese medicine composition is prepared from the following raw medicinal materials by weight: raw aconite 5-15 parts, fried licorice 10-120 parts, dried ginger 5-50 parts, bupleurum 5-50 parts, paeony 10-80 parts, citron fruit 5-50 parts and chuanxiong 2-12 parts.

[0011] The preparation method of the anti-Parkinson's traditional Chinese medicine composition for warming the yang and relieving the stagnation according to the embodiment of the application comprises the following steps:

[0012] According to the above proportion of the traditional Chinese medicine, after soaking in water, the first decoction is carried out; after filtration, water is added to the dregs to carry out the second decoction;

[0013] The decoction liquid obtained by combining the two decoctions.

[0014] Further, according to the preparation method of the traditional Chinese medicine composition of the embodiment of the application, the combined decoction liquid is left to settle, water bath decoction is concentrated to obtain concentrated medicine.

[0015] The preparation method of the traditional Chinese medicine composition according to the embodiment of the application comprises the following steps:

[0016] According to the above proportion of traditional Chinese medicine, the first decoction is added with 10 times the amount of water of the total mass of traditional Chinese medicine, soaked for 30 min, boiled with strong fire, then decocted for 90 min, filtered with gauze; the second decoction is added with 8 times the amount of water, boiled, then decocted for 80 min, filtered with gauze, the filtrates of two times are combined, and the supernatant is taken after standing for 12 h, the decoctions of two times are combined, and concentrated by water bath decoction to obtain concentrated medicine.

[0017] Preferably, the traditional Chinese medicine composition is made of the following raw medicines by weight: raw aconite 10-15 parts, fried licorice 20-80 parts, dried ginger 10-30 parts, bupleurum 10-30 parts, peony 20-60 parts, bitter orange fruit 10-30 parts, chuanxiong 4-10 parts.

[0018] More preferably, the traditional Chinese medicine composition is made of the following raw medicines by weight: raw aconite 15 parts, fried licorice 30 parts, dried ginger 24 parts, bupleurum 15 parts, peony 30 parts, bitter orange fruit 15 parts, chuanxiong 9 parts.

[0019] The treatment principle and method of Parkinson's disease in the application:

[0020] In clinical practice, the treatment of Parkinson's disease should grasp the basic pathogenesis of "yang deficiency and liver depression", first warm and tonify "spleen and kidney yang", and consider "soothing liver and regulating qi", and "warming yang, dispelling cold and resolving depression" should be throughout the whole treatment process. At the same time, the yin essence should not be ignored, and the treatment of the root and branch should be combined, the mechanism should be mutual, and the synergistic effect should be considered.

[0021] Prescription composition and prescription explanation

[0022] (1) Composition: aconite, dried ginger, fried licorice, bupleurum, peony, bitter orange fruit, chuanxiong.

[0023] (2) Prescription explanation: aconite, dried ginger and fried licorice are used in large amount, aconite is a group of fire, which tonifies the yang of the whole body, and the kidney and the primordial qi, and the dried ginger is used to warm the middle jiao and the earth, and the licorice is used to cover the fire with earth; the three drugs are used together to tonify the yang of the spleen and kidney, and warm the yang of the whole body. Peony, bitter orange fruit and chuanxiong are used together with bupleurum to soothe and diffuse the meridian qi and qi, and bitter orange fruit is used to open and disperse the stagnation, and the two are used together to ascend and descend and discharge. White peony root and licorice are used together to astringe yin and soothe tendons, and the body and use are considered together. Chuanxiong is used together to regulate the blood and qi inside and outside, up and down. Although it is two prescriptions, it is actually three prescriptions, among which, Sini Decoction contains aconite, dried ginger and fried licorice to tonify yang; Sini Powder contains bupleurum, peony, bitter orange fruit and fried licorice to regulate qi; the first two prescriptions contain peony and licorice, which also have the meaning of peony and licorice decoction, which is used to soften yin; together, it can warm yang and resolve depression, soften tendons and regulate qi, and consider the body and use.

[0024] (3) Pharmacological action

[0025] Shengfuzi: It belongs to heart, kidney and spleen meridians. It is pungent, sweet and extremely hot, and toxic. It has the functions of restoring yang, rescuing from collapse, tonifying fire and assisting yang, dispelling cold and relieving pain. Modern pharmacological studies have shown that Fuzi contains more than 100 chemical components, including alkaloids and non-alkaloids. The main bioactive components are diterpene alkaloids, flavonoids, and steroidal saponins, which have the effects of protecting the heart, regulating immunity, anti-tumor, protecting nerve injury, and anti-depression. Fuzi plays a role in the signal transduction of MAPK1 / MAPK3 related to neuron survival. It can promote the expression of GRIN1 and MAPK1 genes by activating the norepinephrine system, and inhibit nerve injury. Fuzi water extract (Norepinephrine) can increase norepinephrine, affect synaptic plasticity, and improve learning and cognitive ability.

[0026] Ganjiang: It belongs to spleen, stomach, kidney, heart and lung meridians. It is hot and pungent in nature. It has the functions of warming middle-jiao, dispelling cold, restoring yang and unblocking collaterals, drying dampness and resolving phlegm. The main chemical components of Ganjiang are volatile oils, gingerols and shogaols. It has the pharmacological effects of analgesic and anti-inflammatory, anti-tumor, anti-pathogen, liver protection and bile secretion, anti-ulcer, and improvement of cardiovascular system. Ganjiang is pungent and hot, containing volatile oils and other pungent components, which can promote local blood circulation, protect the wound and promote healing. The water extract and volatile oil of Ganjiang have the effects of inhibiting platelet aggregation and preventing thrombosis. Ganjiang extract has a biphasic regulation effect on immune function. Studies have found that the enhancing effect of Ganjiang extract on cytokines is time-dependent, and its anti-inflammatory effect is related to the gingerols and diphenylheptane components. 6-gingerol can relieve systemic and local inflammatory reactions by regulating the balance of Th17 / Treg cells, and has therapeutic effect on DSS-induced UC in mice. 10-gingerol can inhibit the expression of pro-inflammatory genes by blocking the activation of NF-κB, thereby reducing the levels of nitric oxide (NO), IL-1B, IL-6 and TNF-α.

[0027] Zhigancao: It is sweet and neutral in nature, and belongs to heart, lung, spleen and stomach meridians. It has the effects of clearing heat and resolving toxins, eliminating phlegm and relieving cough, tonifying spleen and lung, and relieving pain. The chemical components of Zhigancao are diverse, mainly including triterpenoid saponins, flavonoids and polysaccharides. Zhigancao has the pharmacological effects of anti-tumor, anti-depression, anti-inflammatory, anti-arrhythmia, liver protection, anti-oxidation and immune regulation. Flavonoids in licorice have the effects of anti-oxidation, anti-tumor, anti-depression, anti-inflammatory and spasmolysis, among which isoliquiritigenin has the strongest spasmolysis effect. Glycyrrhizin can effectively reduce the levels of pro-inflammatory cytokines in lipopolysaccharide-induced mouse depression model, reduce the expression of NF-KB / p-p65 and p-IKBα, and increase the content of brain-derived neurotrophic factor (BDNF) and tyrosine kinase receptor B (TrkB).

[0028] Radix Bupleuri: It belongs to liver and gallbladder meridians. It is acrid, bitter and cold in nature, and has the functions of dispersing heat, resolving the exterior, resolving the interior, regulating menstruation, and lifting yang. Saikosaponin is the main component of Radix Bupleuri. More than 100 saikosaponin components have been confirmed, among which saikosaponin is a popular compound in recent years, and has the effects of anti-inflammatory, antiviral, antipyretic, analgesic, antiepileptic, antitumor, and hepatoprotective. Flavonoids are mainly found in the aboveground parts of plants in the genus Bupleurum. Kaempferol, isorhamnetin, and quercetin are the main three types, and have the effects of protecting nerves, antioxidant, anti-depression, and inhibiting the central nervous system. The effective components of Radix Bupleuri can improve nerve function, inhibit nerve cell death, and protect nerves. Total saikosaponin can improve learning and memory disorders by increasing the expression of brain-derived neurotrophic factor and related pathways, and promoting the occurrence of nerve synapses. In addition, the effective components of Radix Bupleuri can inhibit oxidative stress by inhibiting low-density lipoprotein, promoting mitochondrial autophagy, increasing the levels of superoxide dismutase and glutathione in the hippocampus, and protecting vascular endothelial cells, thereby protecting against ischemia-reperfusion brain injury.

[0029] Fructus Aurantii Immaturus: It belongs to the spleen and stomach meridians. It is bitter, acrid, sour, and cold in nature. It has the functions of resolving phlegm and eliminating stagnation. Its chemical components mainly include flavonoids, volatile oils, and alkaloids, which have the effects of regulating the gastrointestinal tract, antioxidant, and anti-inflammatory. Flavonoids in Fructus Aurantii Immaturus play a major role in antioxidant, which is one of the main mechanisms for treating AD.

[0030] Radix Paeoniae: It belongs to the liver and spleen meridians. It is sour, bitter, and cold in nature. It has the effects of nourishing blood, regulating menstruation, astringing yin, and calming liver yang. It is rich in monoterpenoid glycosides, triterpenes, flavonoids, tannins, volatile oils, and phenolic acids, and has the effects of anti-inflammatory, hepatoprotective, antihypertensive, analgesic, antioxidant, antidepressant, and immunomodulatory, and can be used to treat cardiovascular and cerebrovascular diseases, neurodegenerative diseases, and other diseases. Studies have shown that paeoniflorin can treat neurodegenerative diseases related to oxidative stress, such as Parkinson's disease, by blocking the mitochondrial pathway. For 6-hydroxydopamine (6-OHDA)-induced PC12 cells, paeoniflorin can protect PC12 cells from apoptosis by reducing the translocation of nuclear factor-kB (NF-kB), increasing the expression of anti-apoptotic cell bcl-2, reducing the expression of pro-apoptotic cell Bax, and preventing cytochrome C from being released from mitochondria to the cytoplasm.

[0031] Chuanxiong: warm in nature, pungent in taste, and belongs to liver meridian, gallbladder meridian and pericardium meridian. It has the effects of activating blood and promoting qi, dispelling wind and relieving pain. Its chemical components mainly include volatile oil, alkaloids, polysaccharides, etc., including phthalides and their dimers, alkaloids, organic acid phenols, polysaccharides, and cerebrosides and ceramides and other compounds; it has various pharmacological activities on cardiovascular system, liver and kidney system, nervous system, respiratory system and other systems, mainly showing the effects of analgesia, anti-inflammatory, antioxidant capacity, antitumor, anticoagulation, antidepressant, anti-aging, anti-atherosclerosis, cell protection, improvement of cardiac function, etc.

[0032] The application provides application of the anti-Parkinson's disease traditional Chinese medicine composition in preparation of an anti-Parkinson's disease medicine.

[0033] According to the anti-Parkinson's disease medicine in the specific embodiment of the application, the traditional Chinese medicine composition is included.

[0034] Preferably, the medicine is prepared from the traditional Chinese medicine composition and pharmaceutically acceptable adjuvants or auxiliary ingredients.

[0035] Preferably, the term "pharmaceutically acceptable" refers to a substance suitable for use with humans and / or animals without undue adverse side effects (such as toxicity, irritation, and allergic response), i.e., the material has a reasonable benefit / risk ratio.

[0036] Preferably, the preparation is an oral preparation.

[0037] Preferably, the preparation is a decoction, a pill, a powder, a paste, a wine, a granule, a tablet or a capsule.

[0038] Preferably, the dosage form of the traditional Chinese medicine composition is not particularly limited, and can be any dosage form suitable for mammals: a decoction, a pill, a powder, a paste, a wine, a granule, a tablet or a capsule. From the standpoints of ease of preparation, administration or ingestion, the preferred traditional Chinese medicine composition is a solid composition, especially a tablet, a granule and a solid-filled or liquid-filled capsule. Oral administration is preferred.

[0039] Preferably, various conventional carriers or adjuvants required in preparation of different dosage forms can be added to the traditional Chinese medicine composition, such as fillers (such as starch), flavoring agents (such as stevioside), antioxidants or coating materials, etc. Conventional traditional Chinese medicine preparation methods can be used to prepare any commonly used dosage form, such as tablets, granules, capsules, pills, etc.

[0040] The traditional Chinese medicine composition of the application can also contain other optional medicinal materials or medicinal material extracts.

[0041] The dosage of the traditional Chinese medicine composition of the present application can vary with the mode of administration, the dosage form and the severity of the disease to be treated. The dosage regimen can be adjusted to provide the optimal therapeutic effect, and the specific dosage should also consider the administration method, the physical condition of the administration subject and other factors, which are within the skill of the art.

[0042] The present application has the following advantages:

[0043] The traditional Chinese medicine composition of the present application is composed of seven medicines, i.e., Fuzi, Ganjiang, Zhigancao, Chaihu, Shaoyao, Cishui and Chuanqiong. The four-reversing decoction is used to warm yang and tonify deficiency, and the effects on both the symptoms and the root cause are considered. The synergistic effect of the four-reversing decoction and the four-reversing powder significantly improves the curative effect on Parkinson's disease.

[0044] The traditional Chinese medicine composition of the present application is made of pure traditional Chinese medicine, and has less toxic and side effects. BRIEF DESCRIPTION OF DRAWINGS

[0045] In order to more clearly illustrate the technical solutions of the embodiments of the present application or the prior art, the following will briefly introduce the drawings needed to be used in the embodiments or the prior art description. Obviously, the drawings in the following description only show some embodiments of the present application, and other drawings can be obtained by those skilled in the art without creative effort.

[0046] Figure 1 Results of sugar water preference test in animal experiment are shown.

[0047] Figure 2 Results of open field test in animal experiment are shown.

[0048] Figure 3 Results of rotation fatigue test in animal experiment are shown.

[0049] Figure 4 Results of Parkinson's tremor score in animal experiment are shown. DETAILED DESCRIPTION

[0050] In order to make the objects, technical solutions and advantages of the present application clearer, the technical solutions of the present application will be described in detail below. Obviously, the described embodiments are only some of the embodiments of the present application, not all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative effort are within the scope of the present application.

[0051] Embodiment 1

[0052] A traditional Chinese medicine composition for treating Parkinson's disease, which comprises Fuzi 5 parts, Zhigancao 10 parts, Ganjiang 5 parts, Chaihu 5 parts, Shaoyao 10 parts, Cishui 5 parts and Chuanqiong 2 parts.

[0053] According to the above-mentioned proportion of traditional Chinese medicine, the first decoction is added with 10 times the amount of water of the total mass of traditional Chinese medicine, soaked for 30 min, boiled with strong fire, then decocted for 90 min, filtered with gauze; the second decoction is added with 8 times the amount of water, boiled, then decocted for 80 min, filtered with gauze, the filtrates of two times are combined, and stand for 12 h, take the supernatant, combine the two decoctions, water bath decoction and concentration, to obtain concentrated medicine.

[0054] Example 2

[0055] A traditional Chinese medicine composition for treating Parkinson's disease, which comprises 5-15 parts of raw aconite, 10-120 parts of fried licorice, 50 parts of dried ginger, 50 parts of bupleurum, 80 parts of peony, 30 parts of bitter orange fruit and 12 parts of chuanxiong.

[0056] According to the above-mentioned proportion of traditional Chinese medicine, the first decoction is added with 10 times the amount of water of the total mass of traditional Chinese medicine, soaked for 30 min, boiled with strong fire, then decocted for 90 min, filtered with gauze; the second decoction is added with 8 times the amount of water, boiled, then decocted for 80 min, filtered with gauze, the filtrates of two times are combined, and stand for 12 h, take the supernatant, combine the two decoctions, water bath decoction and concentration, to obtain concentrated medicine.

[0057] Example 3

[0058] A traditional Chinese medicine composition for treating Parkinson's disease, which comprises 15 parts of raw aconite, 30 parts of fried licorice, 24 parts of dried ginger, 15 parts of bupleurum, 30 parts of peony, 15 parts of bitter orange fruit and 9 parts of chuanxiong.

[0059] According to the above-mentioned proportion of traditional Chinese medicine, the first decoction is added with 10 times the amount of water of the total mass of traditional Chinese medicine, soaked for 30 min, boiled with strong fire, then decocted for 90 min, filtered with gauze; the second decoction is added with 8 times the amount of water, boiled, then decocted for 80 min, filtered with gauze, the filtrates of two times are combined, and stand for 12 h, take the supernatant, combine the two decoctions, water bath decoction and concentration, to obtain concentrated medicine.

[0060] Example 4

[0061] A mouse model of Parkinson's disease combined with depression is prepared by injecting MPTP into the abdominal cavity after modeling Parkinson's disease and then performing chronic unpredictable mild stress.

[0062] According to the drug concentration of 30 mg / kg, MPTP is injected into the abdominal cavity of mice at 0.2 ml each time, for a total of 7 days. According to the reference "chronic unpredictable mild stress", including fasting, water deprivation, continuous 24-hour lighting, black and white reversal, inclined empty cage, empty cage with water at the bottom, 2-3 kinds of stimulation are taken every day, for a total of 21 days.

[0063] After the end of the modeling, the western medicine treatment group was given pramipexole at 0.5 mg / kg by gavage, the traditional Chinese medicine treatment group was given the composition of the application by gavage, the high-dose group (8.97 mg / kg) and the low-dose group (4.48 mg / kg), for a total of 21 days. After the experiment, the influence of traditional Chinese medicine treatment on anxiety, depression and movement disorders of mice was evaluated through the sucrose preference test, open field test, rotarod test and tremor paralysis score test.

[0064] Sucrose preference test (SPT)

[0065] After the experimental mice were raised individually for 24 h, two bottles of 1% sucrose solution were placed in the feeding cage for 12 h of adaptive drinking, then one bottle of 1% sucrose solution and one bottle of distilled water were placed in the feeding cage, the positions of the sucrose solution and distilled water were exchanged after 12 h, and this process was continued for 12 h. After adaptive drinking, the experimental mice were deprived of water for 12 h, then one bottle of 1% sucrose solution and one bottle of distilled water were placed in the feeding cage for 12 h of drinking. Before and after the experiment, the weights of the sucrose solution and distilled water were measured, and the sucrose consumption ratio was calculated using the following formula: sucrose preference index = sucrose consumption amount / (sucrose consumption amount + water consumption amount).

[0066] Open field test (OFT)

[0067] After the mice were adapted to the laboratory environment for 1 h, they were placed in a 40 cm×40 cm×35 cm open field box, and a 20×20 cm area near the center of the box was set as the central area. The total movement distance of the mice within 5 min before the experiment, the number of times of entering the central area (central area entry times), and the time of staying in the central area (central area stay time) were recorded. The open field box was cleaned with 75% alcohol before the open field test of the next batch of mice. The TopScanTM2.0 software of the American Clever Sys Inc company was used to analyze the behavior data of the mice.

[0068] Rotarod test (RT)

[0069] The rotarod test requires animals to balance and move continuously on a rotating rod, and is a widely used test for motor coordination. The rotarod fatigue instrument consists of a rotating rod and four independent compartments. Each rat is placed on the rotarod instrument, and the rotation speed is accelerated from 5r to 40r within 1 min until the rat falls. Each rat is trained for 4 times, and trained for 3 days before the experiment. In the formal rotarod test, the rat is placed on the rotarod instrument for 10s, and when it can walk steadily and continuously balance, the recording is started. The rotation speed of the rotarod instrument is accelerated from 5r to 40r within 1 min, and the time of the rat staying on the rotating rod is recorded as the latency (i.e. the time from the start of the rotating rod to the rat falling). If the rat does not fall, the maximum value of 300s is recorded. After each test, the rat is placed back in the cage for 1 min of rest, and the test is continuously performed for 7 times. The maximum and minimum values are removed, and the average value of the remaining 5 results is taken.

[0070] Paralysis tremor score (PTS)

[0071] The mouse paralysis tremor score is based on the mouse phenotype characteristics, including hair (bundle hair), motor performance (walking, shaking, deflection), limb activity (arching, tremor, tail tremor, nodding, stiffness), and spontaneous behavior (swallowing, salivation). The grade score is divided into 5 grades, 0 represents no adverse reactions, similar to normal mice (no); 1 indicates bundle hair, arching, fine tremor, etc. reactions, but do not affect the activity (occasionally); 2 indicates frequent swallowing, limb tremor, tail tremor, hind limb opening, etc. reactions, affecting activity (intermittently); 3 means persistent limb tremor, stiffness, salivation, etc., unable to move normally (persistent); 4 is persistent paralysis and severe death (severe). The mouse is transferred to an empty feeding cage, the environment is quiet, the light is appropriate, the cage is cleaned with alcohol each time, the observer does not carry irritating odor, and does not make abnormal sound. The observation process lasts for 10 min, the first 5 min is the adaptation stage, and the last 5 min is the formal observation stage; each animal is observed by two observers, and if the results are different, a third observer is asked to score.

[0072] Results: After the experiment, take Parkinson behavior evaluation method tremor score, rotation fatigue test, depression behavior evaluation method open field test, sugar water preference test. The results of the behavior show that after modeling, the mice show a significant decrease in sugar water preference, the total distance of open field movement is shortened, indicating that the mice have depression-like symptoms. And the tremor score of the normal mice in the blank control group is significantly increased, the residence time in the rotating rod test is significantly reduced, indicating that the mice have significant motor disorders. After treatment, compared with the model group, the traditional Chinese medicine group improves the sugar water preference rate of the mice, the total distance of the open field movement, indicating that the depression-like symptoms of the mice are reduced after treatment; and the tremor score of the traditional Chinese medicine group is lower, similar to that of the normal mice in the blank control group, the residence time in the rotating rod test is increased, indicating that the Parkinson-like behavior of the mice is reduced after treatment. The high and low dose experiments of traditional Chinese medicine show that the traditional Chinese medicine composition of the application significantly improves various behavior tests, and shows a dose-dependent manner, indicating that the composition of the application significantly improves the depression and motor disorders of the mice.

[0073] The above is only a specific embodiment of the present application, but the protection scope of the present application is not limited thereto, any person skilled in the art can easily think of changes or replacements within the technical range disclosed by the present application, which should be covered within the protection scope of the present application. Therefore, the protection scope of the present application should be subject to the protection scope of the claims.

Claims

1. A traditional Chinese medicine composition for treating Parkinson's disease depression and warming yang to relieve depression, characterized in that, The traditional Chinese medicine composition is made of raw aconite 10-15 parts, prepared licorice 20-80 parts, dried ginger 10-30 parts, bupleurum 10-30 parts, peony 20-60 parts, bitter orange fruit 10-30 parts and chuanxiong 4-10 parts by weight.

2. The traditional Chinese medicine composition according to claim 1, characterized in that, The traditional Chinese medicine composition is made of raw aconite 15 parts, prepared licorice 30 parts, dried ginger 24 parts, bupleurum 15 parts, peony 30 parts, bitter orange fruit 15 parts and chuanxiong 9 parts by weight.

3. Use of the traditional Chinese medicine composition of any one of claims 1-2 in the preparation of a medicine for resisting depression of Parkinson's disease.

4. An anti-Parkinsonian antidepressant drug, characterized by comprising a compound of the formula (I) as an effective ingredient. The medicine contains the traditional Chinese medicine composition of any one of claims 1-2.

5. The anti-Parkinson's disease and depression drug according to claim 4, characterized in that, The medicine contains pharmaceutically acceptable adjuvants.

6. The anti-Parkinson's disease and antidepressant drug according to claim 4, characterized in that, The medicine is an oral preparation.

7. The anti-Parkinson's disease and antidepressant drug according to claim 4 or 6, characterized in that, The medicine is a decoction, a pill, a powder, a paste, a liquor, a granule, a tablet or a capsule.

Images