Automatic flow cytometry device

Abstract

The invention discloses an automatic flow cytometry staining device which comprises a base, a staining assembly is arranged on the base and comprises a plurality of storage tanks arranged on the base, the storage tanks are filled with fluorescent staining agents, a centrifugal machine is arranged on one side of the base, and two pairs of guide rails are arranged on the base. One end of the base is connected with one end of the sliding block through a first telescopic rod, a movable frame is arranged at the top end of the sliding block, a centrifugal tube is clamped on the movable frame, and a liquid guide assembly is arranged at the top end of each storage tank; according to the invention, the arranged dyeing assembly can select a fluorescent dyeing agent, a tester does not need to select and drop the dyeing agent by himself, the test efficiency is further improved, before the dyeing agent is dropped, names of different target molecules need to be input into a computer, and the computer can select the fluorescent dyeing agent, so that the test efficiency is improved. And a specified amount of corresponding coloring agent can be added by moving the centrifugal tube.

Description

Technical Field

[0001] The present invention relates to the technical field of cell staining, and more specifically, to an automated flow cell staining device. Background Art

[0002] Flow cytometry is a multi-parameter, rapid quantitative analysis of single cells or other biological particles at the cellular molecular level using monoclonal antibodies. In order to quickly identify and analyze target cells, it is usually necessary to stain the target cells before flow cytometry analysis. The staining steps are specified based on the expression site of the target (cell surface or intracellular). When the target is on the cell surface, fixation and permeabilization are not required, and living cells can be directly tested and analyzed. When the target is inside the cell, the cell needs to be fixed and permeabilized for intracellular staining.

[0003] However, when multiple molecules need to be stained at the same time, since molecules with high expression levels of the target molecules require the use of weaker fluorescent substances, most existing flow cytometer staining devices require manual selection of fluorescent antibodies, which increases the burden on personnel during the staining operation and reduces the efficiency of the staining operation. Summary of the invention

[0004] The present invention provides an automated flow cell staining device to solve the problem that most flow cell staining devices in the prior art require manual selection of fluorescent antibodies, which increases the burden on personnel during the staining operation and reduces the efficiency of the staining operation.

[0005] In order to achieve the above object, the present invention provides the following technical solutions: An automated flow cytometer staining device comprises a base, wherein a staining assembly is arranged on the base, wherein the staining assembly comprises a plurality of storage tanks arranged on the base, wherein the storage tanks are filled with fluorescent stains, wherein a centrifuge is arranged on one side of the base, wherein two pairs of guide rails are provided on the base, wherein a setting direction of one pair of guide rails is the same as an arrangement direction of the storage tanks, and the two pairs of guide rails are arranged vertically, wherein a slider is provided on the guide rails, wherein one end of the base is connected to one end of the slider via a first telescopic rod, wherein the first telescopic rod is controlled by a computer, wherein a movable rack is provided on the top of the slider, wherein a centrifuge tube is clamped on the movable rack, and wherein a liquid guide assembly is provided on the top of the storage tank.

[0006] Preferably, the liquid guiding component includes a connecting sleeve arranged on the outside of the storage tank, a sliding plate is slidably clamped in the connecting sleeve, the top of the sliding plate is rotatably connected to a connecting block through a coil spring, the bottom of the sliding plate is connected to a limiting block, one side of the connecting block is connected to a top cover, the top cover is arranged on the top of the storage tank, and the limiting plates arranged at both ends of the connecting block are respectively slidably clamped in the grooves opened on both sides of the connecting sleeve.

[0007] Preferably, a sealing ring is annularly arranged at the bottom end of the top cover, a transfer tube is arranged on the inner wall of the top cover, a liquid drawing tube is arranged on the inner wall of the storage tank, the top end of the liquid drawing tube is connected to the bottom end of the transfer tube, a sleeve is connected to the outside of the transfer tube, and the sleeve is arranged on the outside of the storage tank.

[0008] Preferably, a first spring is disposed on the inner top wall of the sleeve, a free end of the first spring is connected to a first connecting tube, and a connecting port is formed on a side of the first connecting tube close to the transfer tube.

[0009] Preferably, the top of the movable frame is rotatably connected to a rotating block via a coil spring, the rotating block is hollow and has a second connecting tube connected to the top, the top of the second connecting tube contacts the bottom end of any one of the first connecting tubes via an inclined surface, an air bag is provided on the bottom wall of the storage tank, the air pump provided in the base is connected to the air bag via a pipeline, and the air pump is controlled by a computer.

[0010] Preferably, the rotating block is provided with an opening at one end facing the centrifuge tube, a guide wheel is rotatably provided on the outer side of the movable frame, a pull rope is connected to the bottom end of the rotating block, a free end of the pull rope is connected to a holder, a magnetic block is provided at one end of the holder, the bottom end of the holder is connected to the slider through a second spring, and the bottom end of the centrifuge tube is in contact with the top end of the holder.

[0011] Preferably, a through hole is provided at the connection between the two pairs of guide rails, a rotating plate is rotatably provided in the through hole, a pair of guide rails is also provided on the rotating plate, and a connecting button is provided at the top of the rotating plate.

[0012] Preferably, a second telescopic rod slidably connected is provided on one side of the base, a sleeve provided at an output end of the second telescopic rod is sleeved on the connecting button, and the second telescopic rod is connected to the inner wall of the base via a third spring.

[0013] Preferably, a third telescopic rod is provided on the base, a connecting frame is provided on the top of the third telescopic rod, one end of the internal channel of the connecting frame is connected to a suction device, and the free end of the internal channel of the connecting frame is connected to a pump through a pipeline.

[0014] Preferably, a ray source and a receiver are arranged at the bottom end of the connecting frame, and the ray source, the receiver and the bottom opening of the absorber are arranged on the same horizontal line.

[0015] The principle and beneficial effects of this technical solution: (1) The dyeing component provided in the present invention can select the fluorescent dye, and the experimenter does not need to select and drip the dye by himself, thereby improving the test efficiency. Before dripping the dye, the name of the different target molecules to be dyed must be input into the computer. The computer will select the fluorescent dye according to the molecular expression corresponding to the molecular name to be dyed. After the selection is completed, the air pump is controlled according to the amount of fluorescent agent added. At this time, the air pump will quantitatively inflate the air bag in the storage tank storing the corresponding fluorescent agent through the pipeline system. At this time, the pressure in the storage tank increases, and the liquid level in the liquid pump tube increases. The first connecting tube is connected to the movable frame by a spring and the second connecting tube is connected to the movable frame by a spring. The first connecting tube is connected to the movable frame by a spring and the first connecting tube is connected to the movable frame by a spring. The ...

[0016] (2) The liquid guide assembly provided in the present invention can prevent the dye from overflowing when the user adds the dye. When the dye needs to be added to the storage tank, the top cover is lifted upwards. At this time, the sliding plate connected to the outer side of the top cover by the connecting block will move up, and the limit plates provided at both ends of the connecting block will gradually slide out of the slot. When the limit plates completely slide out of the slot, since a coil spring is provided at the hinge between the connecting block and the sliding plate, the coil spring will drive the connecting block and the top cover to rotate to a vertical direction. At the same time, the transfer tube provided on the inner wall of the top cover will also be disconnected from the liquid pumping tube when the top cover moves up, thereby preventing the fluorescent agent from overflowing.

[0017] (3) The absorber provided in the present invention can absorb the supernatant after centrifugation. After centrifugation is completed by the centrifuge and the centrifuge tube is reloaded onto the movable rack, and then the second telescopic rod is shortened. The second telescopic rod will pull the connecting button provided at the top of the rotating plate through the sleeve provided at the output shaft end, so that the rotating plate rotates in the through hole until the rotating plate drives the slider and the movable rack to rotate 90 degrees. At this time, the guide rail provided on the rotating plate is aligned with the other pair of guide rails, driving the slider and the movable rack to slide to the bottom of the absorber, turning on the radiation source and the receiver, and then driving the third telescopic rod to shorten. At this time, the connecting rack and the absorber connected to the top of the third telescopic rod will move downward synchronously. When the light intensity received by the receiver drops significantly, the third telescopic rod is stopped and the pump is started. The pump will suck out the supernatant through the pipeline and the absorber. After the absorption is completed, the third telescopic rod is reset and the centrifuge tube is removed. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a schematic diagram of the overall structure of the present invention; Figure 2It is a schematic diagram of the structure after the present invention is split; Figure 3 for Figure 2 Schematic diagram of the enlarged structure of the A area in the middle; Figure 4 for Figure 2 Schematic diagram of the enlarged structure of the middle B area; Figure 5 for Figure 2 Schematic diagram of the enlarged structure of the middle C area; The figure marks in the drawings of the specification include: 1, storage tank; 2, base; 3, centrifuge; 4, first telescopic rod; 5, connecting frame; 6, absorber; 7, radiation source; 8, receiver; 9, third telescopic rod; 10, pump; 11, transfer tube; 12, top cover; 13, limit plate; 14, connecting block; 15, sliding plate; 16, limit block; 17, slot; 18, connecting sleeve; 19, sleeve; 20, first spring; 21, first connecting tube; 22, connecting port; 23, sealing ring; 24, liquid drawing tube; 25, second connecting tube; 26, rotating block; 27, pull rope; 28, moving frame; 29, guide wheel; 30, seat; 31, second spring; 32, centrifuge tube; 33, slider; 34, rotating plate; 35, sleeve; 36, guide rail; 37, through hole; 38, second telescopic rod; 39, third spring. DETAILED DESCRIPTION

[0019] The present invention is further described in detail below in conjunction with the accompanying drawings and embodiments: Embodiment:

[0020] like Figures 1 to 5 As shown, the present invention provides an automated flow cytometry staining device, including a base 2, on which a staining assembly is arranged, the staining assembly includes a plurality of storage tanks 1 arranged on the base 2, the storage tanks 1 are filled with fluorescent stains, a centrifuge 3 is arranged on one side of the base 2, two pairs of guide rails 36 are opened on the base 2, wherein a pair of guide rails 36 are arranged in the same direction as the arrangement direction of the storage tanks 1, the two pairs of guide rails 36 are arranged vertically, a slider 33 is slidably provided on the guide rails 36, one end of the base 2 is connected to one end of the slider 33 through a first telescopic rod 4, the first telescopic rod 4 is controlled by a computer, a movable frame 28 is arranged on the top of the slider 33, a centrifuge tube 32 is clamped on the movable frame 28, and a liquid guide assembly is arranged on the top of the storage tank 1.

[0021] like Figure 1 and Figure 3As shown, the liquid guiding assembly includes a connecting sleeve 18 arranged on the outside of the storage tank 1, a sliding plate 15 is slidably clamped in the connecting sleeve 18, the top end of the sliding plate 15 is rotatably connected to a connecting block 14 through a coil spring, the bottom end of the sliding plate 15 is connected to a limiting block 16, one side of the connecting block 14 is connected to a top cover 12, the top cover 12 is covered on the top of the storage tank 1, and the limiting plates 13 arranged at both ends of the connecting block 14 are respectively slidably clamped in the card grooves 17 opened on both sides of the connecting sleeve 18.

[0022] like Figure 1 and Figure 3 As shown, a sealing ring 23 is annularly arranged at the bottom end of the top cover 12, a transfer tube 11 is arranged on the inner wall of the top cover 12, a liquid drawing tube 24 is arranged on the inner wall of the storage tank 1, the top end of the liquid drawing tube 24 is connected to the bottom end of the transfer tube 11, a sleeve 19 is connected to the outside of the transfer tube 11, and the sleeve 19 is arranged on the outside of the storage tank 1.

[0023] The liquid guiding component can prevent the dye from overflowing when the user adds the dye. When the dye needs to be added to the storage tank 1, the top cover 12 is lifted upwards. At this time, the sliding plate 15 connected to the outside of the top cover 12 by the connecting block 14 will move up, and the limiting plates 13 set at both ends of the connecting block 14 will gradually slide out of the slot 17. When the limiting plate 13 completely slides out of the slot 17, since a coil spring is set at the hinge between the connecting block 14 and the sliding plate 15, the coil spring will drive the connecting block 14 and the top cover 12 to rotate to a vertical direction. At the same time, the transfer tube 11 set on the inner wall of the top cover 12 will also be disconnected from the liquid drawing tube when the top cover 12 moves up, thereby preventing the fluorescent agent from overflowing.

[0024] like Figure 3 As shown, a first spring 20 is disposed on the inner top wall of the sleeve 19 , a first connecting tube 21 is connected to the free end of the first spring 20 , and a connecting port 22 is formed on the side of the first connecting tube 21 close to the transfer tube 11 .

[0025] like Figure 1 and Figure 4 As shown, the top of the movable frame 28 is rotatably connected to a rotating block 26 through a coil spring. The rotating block 26 is hollow and has a second connecting tube 25 connected to the top. The top of the second connecting tube 25 contacts the bottom end of any first connecting tube 21 through an inclined surface. An air bag is arranged on the bottom wall of the storage tank 1. The air pump arranged in the base 2 is connected to the air bag through a pipeline, and the air pump is controlled by a computer.

[0026] like Figure 4 As shown, an opening is provided at one end of the rotating block 26 facing the centrifuge tube 32, a guide wheel 29 is rotatably provided on the outer side of the movable frame 28, a pull rope 27 is connected to the bottom end of the rotating block 26, a free end of the pull rope 27 is connected to a holder 30, a magnetic block is provided at one end of the holder 30, the bottom end of the holder 30 is connected to the slider 33 through a second spring 31, and the bottom end of the centrifuge tube 32 is in contact with the top end of the holder 30.

[0027] like Figure 5As shown, a through hole 37 is provided at the connection of the two pairs of guide rails 36, and a rotating plate 34 is rotatably mounted in the through hole 37. A pair of guide rails 36 is also provided on the rotating plate 34, and a connecting button is provided at the top of the rotating plate 34.

[0028] like Figure 1 and Figure 5 As shown, a second telescopic rod 38 is slidably connected to one side of the base 2, a sleeve 35 provided at the output end of the second telescopic rod 38 is sleeved on the connecting button, and the second telescopic rod 38 is connected to the inner wall of the base 2 through a third spring 39.

[0029] like Figure 1 and Figure 2 As shown, a third telescopic rod 9 is provided on the base 2, a connecting frame 5 is provided on the top of the third telescopic rod 9, one end of the internal channel of the connecting frame 5 is connected to a suction device 6, and the free end of the internal channel of the connecting frame 5 is connected to a pump 10 through a pipeline.

[0030] like Figure 2 As shown, a ray source 7 and a receiver 8 are disposed at the bottom of the connecting frame 5, and the ray source 7, the receiver 8 and the bottom opening of the absorber 6 are disposed on the same horizontal line.

[0031] The absorber 6 can absorb the supernatant after centrifugation. After centrifugation is completed by the centrifuge 3 and it is left to stand for a period of time, the centrifuge tube 32 is reloaded onto the mobile frame 28, and then the second telescopic rod 38 is shortened. The second telescopic rod 38 will pull the connecting button set at the top of the rotating plate 34 through the sleeve 35 set at its output shaft end, so that the rotating plate 34 rotates in the through hole 37 until the rotating plate 34 drives the slider 33 and the mobile frame 28 to rotate 90 degrees. At this time, the guide rail 36 provided on the rotating plate 34 is aligned with the other pair of guide rails 36, driving the slider 33 and the mobile frame 28 to slide to the bottom of the absorber 6, turning on the radiation source 7 and the receiver 8, and then driving the third telescopic rod 9 to shorten. At this time, the connecting frame 5 and the absorber 6 connected to the top of the third telescopic rod 9 will move downward synchronously. When the light intensity received by the receiver 8 decreases significantly, the third telescopic rod 9 is stopped and the pump 10 is started. The pump 10 will suck out the supernatant through the pipeline and the absorber 6. After the absorption is completed, the third telescopic rod 9 is reset and the centrifuge tube 32 is removed.

[0032] The specific usage and function of this embodiment are as follows: The dyeing component provided in the present invention can select fluorescent dyes, and there is no need for the experimenter to select and drip the dyes by himself, thereby improving the test efficiency. Before dripping the dyes, the names of the different target molecules to be dyed need to be input into the computer first, and the computer will select the fluorescent dyes according to the molecular expression corresponding to the molecular names to be dyed. After the selection is completed, the air pump is controlled according to the amount of fluorescent agent added. At this time, the air pump will quantitatively inflate the airbag in the storage tank 1 storing the corresponding fluorescent agent through the pipeline system. At this time, the pressure in the storage tank increases, and the liquid level in the liquid drawing tube will rise. After the pressurization is completed, the first telescopic rod 4 is driven to shorten by the computer, and the first telescopic rod 4 will drive the slider 33 and the movable frame 28 to slide along the guide rail 36, and the movable frame 28 is clamped. The centrifuge tube 32 will translate along the guide rail 36. At this time, the rotating block 26 and the second connecting tube 25 connected by the coil spring on the moving frame 28 will sequentially squeeze the first connecting tube 21 set on the outside of different storage tanks 1 through the inclined surface. When the second connecting tube 25 squeezes the first connecting tube 21 on the outside of the pressurized storage tank 1, the first connecting tube 21 moves up and compresses the first spring 20. At the same time, the connecting port 22 opened on the side of the first connecting tube 21 close to the transfer tube 11 will be directly connected to the transfer tube 11. At this time, the liquid in the liquid drawing tube will enter the transfer tube 11 under the action of pressure, and finally be introduced into the rotating block 26 connected to the second connecting tube 25 through the transfer tube 11. The opening set at one end of the rotating block 26 toward the centrifuge tube 32 will introduce the fluorescent agent into the centrifuge tube 32 for dyeing. After dyeing is completed, the first telescopic rod 4 is continued to be driven to shorten. After the second connecting tube 25 is misaligned with the first connecting tube 21, the first connecting tube 21 will be reset to block the outflow of the fluorescent agent. When the slider 33 and the movable frame 28 move to above the rotating plate 34, the first telescopic rod 4 stops shortening. At this time, the centrifuge tube 32 is manually pressed down until the holder 30 set at the bottom end of the centrifuge tube 32 is attracted to the slider 33 through the magnetic block. During the downward movement of the holder 30, the pull rope 27 is pulled to make the rotating block 26 connected to the free end of the pull rope 27 rotate along the top of the movable frame 28, and at the same time, the coil spring set at the top of the movable frame 28 is contracted. When the holder 30 is attracted to the slider 33 through the magnetic block, the rotating block 26 will rotate to the vertical direction. At this time, the centrifuge tube 32 is removed and the slider 33 and the movable frame 28 are reset through the first telescopic rod 4. When the device is used again, the holder 30 is manually lifted so that the holder 30 can hold up the centrifuge tube 32.

[0033] The above is only an embodiment of the present invention, and the common knowledge such as the known specific technical solutions and / or characteristics in the solution is not described in detail here. It should be pointed out that for those skilled in the art, without departing from the technical solution of the present invention, several modifications and improvements can be made, which should also be regarded as the protection scope of the present invention, and these will not affect the effect of the implementation of the present invention and the practicality of the patent. The scope of protection required by this application shall be based on the content of its claims, and the specific implementation methods and other records in the specification can be used to interpret the content of the claims.

Claims

1. An automated flow cytometric staining device, characterized in that: The invention comprises a base (2), wherein a dyeing assembly is arranged on the base (2), wherein the dyeing assembly comprises a plurality of storage tanks (1) arranged on the base (2), wherein the storage tanks (1) are filled with fluorescent dye, and a centrifuge (3) is arranged on one side of the base (2). Two pairs of guide rails (36) are provided on the base (2), wherein the arrangement direction of one pair of guide rails (36) is the same as the arrangement direction of the storage tanks (1), and the two pairs of guide rails (36) are arranged vertically, and a slider (33) is provided on the guide rail (36) for sliding, and one end of the base (2) is connected to one end of the slider (33) via a first telescopic rod (4), and the first telescopic rod (4) is controlled by a computer, and a movable frame (28) is provided at the top of the slider (33), and a centrifuge tube (32) is clamped on the movable frame (28), and a liquid guide assembly is provided at the top of the storage tank (1).

2. An automated flow cell staining device according to claim 1, characterized in that: The liquid guide assembly comprises a connecting sleeve (18) arranged outside the storage tank (1), a sliding plate (15) is slidably clamped inside the connecting sleeve (18), the top end of the sliding plate (15) is rotatably connected to a connecting block (14) via a coil spring, the bottom end of the sliding plate (15) is connected to a limiting block (16), one side of the connecting block (14) is connected to a top cover (12), the top cover (12) is covered at the top of the storage tank (1), and the limiting plates (13) arranged at both ends of the connecting block (14) are respectively slidably clamped in the clamping grooves (17) opened on both sides of the connecting sleeve (18).

3. An automated flow cell staining device according to claim 2, characterized in that: A sealing ring (23) is provided in an annular shape at the bottom end of the top cover (12); a transfer tube (11) is provided on the inner wall of the top cover (12); a liquid drawing tube (24) is provided on the inner wall of the storage tank (1); the top end of the liquid drawing tube (24) is connected to the bottom end of the transfer tube (11); the outer side of the transfer tube (11) is connected to a sleeve (19); and the sleeve (19) is provided on the outer side of the storage tank (1).

4. An automated flow cell staining device according to claim 3, characterized in that: A first spring (20) is disposed on the inner top wall of the sleeve (19); a free end of the first spring (20) is connected to a first connecting tube (21); and a connecting port (22) is provided on a side of the first connecting tube (21) close to the transfer tube (11).

5. An automated flow cell staining device according to claim 4, characterized in that: The top of the movable frame (28) is rotatably connected to a rotating block (26) via a coil spring; the rotating block (26) is hollow and has a second connecting tube (25) connected to the top; the top of the second connecting tube (25) contacts the bottom of any one of the first connecting tubes (21) via an inclined surface; an air bag is arranged on the bottom wall of the storage tank (1); an air pump arranged in the base (2) is connected to the air bag via a pipeline; and the air pump is controlled by a computer.

6. An automated flow cell staining device according to claim 5, characterized in that: The rotating block (26) is provided with an opening at one end facing the centrifuge tube (32); a guide wheel (29) is rotatably provided on the outer side of the movable frame (28); a pull rope (27) is connected to the bottom end of the rotating block (26); a free end of the pull rope (27) is connected to a holder (30); a magnetic block is provided at one end of the holder (30); the bottom end of the holder (30) is connected to the slider (33) via a second spring (31); and the bottom end of the centrifuge tube (32) contacts the top end of the holder (30).

7. An automated flow cell staining device according to claim 6, characterized in that: A through hole (37) is provided at the connection between the two pairs of guide rails (36), a rotating plate (34) is rotatably provided in the through hole (37), a pair of guide rails (36) is also provided on the rotating plate (34), and a connecting button is provided at the top of the rotating plate (34).

8. An automated flow cell staining device according to claim 7, characterized in that: A second telescopic rod (38) is slidably connected to one side of the base (2); a sleeve (35) provided at the output end of the second telescopic rod (38) is sleeved on the connecting button; the second telescopic rod (38) is connected to the inner wall of the base (2) via a third spring (39).

9. An automated flow cell staining device according to claim 8, characterized in that: A third telescopic rod (9) is arranged on the base (2), a connecting frame (5) is arranged on the top of the third telescopic rod (9), one end of the internal channel of the connecting frame (5) is connected to a suction device (6), and the free end of the internal channel of the connecting frame (5) is connected to a pump (10) via a pipeline.

10. An automated flow cell staining device according to claim 9, characterized in that: A ray source (7) and a receiver (8) are arranged at the bottom end of the connecting frame (5); the ray source (7), the receiver (8) and the bottom opening of the absorber (6) are arranged on the same horizontal line.

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