Optical system and detection method

Abstract

An optical system and a detection method, the optical system comprises a first lens (10) and a second lens (20) which are sequentially arranged from an object side to an image side along an optical axis direction, the first lens (10) and the second lens (20) are correspondingly arranged, the second lens (20) is configured in a mobile terminal (200), the first lens (10) and the second lens (20) have the same aperture number and the same focal length, and the first lens (10) and the second lens (20) have the same aperture number and the same focal length. An exit pupil of the first lens (10) is positioned at an entrance pupil of the second lens (20), so that the mobile terminal (200) can be used for acquiring image data of an external sample to be detected through the first lens (10) and the second lens (20) in sequence and obtaining a corresponding optical image, and a target base sequence is analyzed and obtained, so that the gene sequencer is more miniaturized, and the detection accuracy is improved. And gene sequencing is more miniaturized, convenient and popular.

Description

Optical system and detection method Technical Field The present invention relates to the field of optics, and in particular to an optical system and a detection method. Background Art In traditional gene sequencing methods, lasers are used to excite samples to produce fluorescence, which is then detected and analyzed by an imaging system consisting of a microscope and a camera to obtain the target base sequence. Currently, gene sequencing needs to be completed by specific institutions such as hospitals, research institutes, and laboratories. This is because current gene sequencers are large in size. Even miniaturized, desktop gene sequencers require professional operators in specific environments to complete. How to make gene sequencing more popular and convenient is an important issue in the field of gene sequencing. Summary of the invention In view of this, an object of the present invention is to provide an optical system and a detection method to popularize and facilitate gene sequencing. To achieve the above object, the present invention has the following technical solutions: The present application provides an optical system, including: A first lens and a second lens are sequentially arranged along the optical axis from the object side toward the image side, the first lens and the second lens are arranged correspondingly, the second lens is configured in a mobile terminal, the first lens and the second lens have the same aperture number and the same focal length, and the exit pupil of the first lens is located at the entrance pupil of the second lens; the mobile terminal is used to collect image data of an external sample to be detected through the second lens and the first lens in sequence and obtain a corresponding optical image. Optionally, the first lens has a first optical element combination, the second lens has a second optical element combination, and the first optical element combination and the second optical element combination are arranged in mirror symmetry with each other. Optionally, the first lens includes a first lens, a second lens, a third lens, a fourth lens and a fifth lens from the object side to the image side, the first lens is a plane mirror, the object side surface of the second lens is concave, and the image side surface is convex, the third lens and the fifth lens are both biconvex lenses, and the object side surface of the fourth lens is a plane, and the image side surface is concave. Optionally, there are multiple first lenses and multiple second lenses, and the multiple first lenses and multiple second lenses are arranged corresponding to each other in pairs, different first lenses have different focal planes, and the different focal planes are parallel to each other. Optionally, the number of the first lens and the second lens is 2, two second lenses are arranged in the same mobile terminal and have different focal lengths; and a line connecting the center points of the two second lenses is parallel to any of the focal planes. Optionally, a filter is arranged between the corresponding first lens and the second lens, and the filter is used to pass light of a preset band and block light of bands outside the preset band. The plane where the filter is located is the symmetric center plane of the corresponding first lens and the second lens. The present application provides a detection method, which includes: A first image is obtained by photographing a first field of view area of ​​a sample chip with an additional lens connected to an external mobile terminal, wherein the mobile terminal has a built-in lens, the additional lens and the built-in lens have the same aperture number and the same focal length, and the exit pupil of the additional lens is located at the entrance pupil of the built-in lens; the mobile terminal and the additional lens are located on one side of the sample chip, and the sample chip can generate a detectable fluorescence signal. Optionally, the method further includes: Before photographing the first field of view area, irradiating the first field of view area from a side of the sample chip away from the mobile terminal with an excitation light source to excite the detectable fluorescent signal; After photographing the first field of view, turning off the excitation light source, and moving the sample chip so that the built-in lens and the additional lens face the second field of view of the sample chip; When the excitation light source is used to illuminate the second field of view area of ​​the sample chip from the side of the sample chip away from the mobile terminal, the second field of view area is photographed by the mobile terminal to obtain a second image. Optionally, the fluorescent signal is generated by a catalytic reaction in the sample chip, and the method further comprises: After photographing the first field of view, the sample chip is moved so that the built-in lens and the additional lens face the second field of view of the sample chip, and the second field of view is photographed by the mobile terminal to obtain a second image. Optionally, a filter is provided between the built-in lens and the additional lens, the filter is used to pass light of a preset band and block light emitted by the excitation light source, and the central wavelength of the fluorescence excited by the sample chip after being irradiated by the excitation light source belongs to the preset band. Optionally, there are multiple built-in lenses and multiple additional lenses, and the multiple built-in lenses and the multiple additional lenses are arranged in one-to-one correspondence, different additional lenses have different focal planes, and the different focal planes are parallel to each other; the first field of view area includes multiple detection areas respectively located at multiple focal planes, and the multiple detection areas are opposite to the multiple additional lenses. Optionally, the number of the additional lens and the built-in lens is 2, the two built-in lenses are arranged in the same mobile terminal and have different focal lengths; a line connecting the center points of the two built-in lenses is parallel to any of the focal planes; The sample chip includes a stacked substrate and a cover plate, and two layers of samples to be tested located between the substrate and the cover plate, the two layers of samples to be tested are respectively located at a first focal plane and a second focal plane, the first focal plane is a surface of the substrate facing the cover plate, and the second focal plane is a surface of the cover plate facing the substrate. Optionally, the additional lens has a first optical element combination, the additional lens has a second optical element combination, and the first optical element combination and the second optical element combination are arranged in mirror symmetry with each other. An embodiment of the present application also provides a portable biochemical testing optical system, including a mobile terminal and an auxiliary mechanism cooperating with the mobile terminal, the mobile terminal having a second lens, the auxiliary mechanism being detachably mounted on the mobile terminal, and the auxiliary mechanism comprising a first lens, the first lens corresponding to the second lens and being mounted on the object side of the second lens, the first lens and the second lens having the same aperture number and the same focal length, the exit pupil of the first lens being located at the entrance pupil of the second lens; the mobile terminal being used to collect detectable signals of an external biochemical sample to be detected through the second lens and the first lens in sequence and obtain corresponding optical images. Optionally, the first lens has a first optical element combination, the second lens has a second optical element combination, and the first optical element combination and the second optical element combination are arranged in mirror symmetry with each other. Optionally, the auxiliary mechanism includes a filter located on a side of the second lens close to the first lens, and the second optical element combination and the second optical element combination are arranged in mirror symmetry with respect to the filter. Optionally, the biochemical sample to be detected is a nucleic acid sequencing library. Optionally, the biochemical sample to be tested is a tissue sample. Optionally, the biochemical sample to be detected undergoes a biochemical reaction with reagents of multiple different reaction components to generate the detectable signal; The reaction components include at least one of a sample generation component or a sample analysis component; Optionally, the biochemical reaction includes controlling reaction conditions so that different sample generating components generate the sample; and The biochemical reaction includes analyzing the sample, including allowing sample analysis components to react with the sample to provide the associated detectable signal. The embodiment of the present application provides a biochemical testing method, comprising: loading a sample to be tested into a channel of a chip circulation pool; and loading a reagent having a plurality of different reaction components into the channel of the chip circulation pool to perform a biochemical reaction between the sample to be tested and the reagent; The reaction components include at least one of a sample generation component or a sample analysis component; Optionally, the biochemical reaction comprises generating a sample in a channel of the chip flow cell, comprising allowing different sample generating components to flow into the channel and controlling reaction conditions of the channel to generate the sample; and The biochemical reaction includes analyzing the sample in the channel, including flowing a sample analysis component into the channel, the sample analysis component reacting with the sample to provide an associated detectable signal; The biochemical testing method also includes: using a portable optical system to identify the detectable signal, the portable optical system includes a mobile terminal and an auxiliary mechanism cooperating with the mobile terminal, the mobile terminal has a second lens, the auxiliary mechanism is detachably installed on the mobile terminal, and the auxiliary mechanism includes a first lens, the first lens corresponds to the second lens and is installed on the object side of the second lens, the first lens and the second lens have the same aperture number and the same focal length, and the exit pupil of the first lens is located at the entrance pupil of the second lens; the mobile terminal collects the detectable signal through the second lens and the first lens in turn and obtains the corresponding optical image. Optionally, the biochemical reaction is a nucleic acid sequencing reaction, and the sample to be detected is a nucleic acid sequencing library. Optionally, the detectable signal is an optical signal. Optionally, the sample to be detected is a tissue sample, and the biochemical reaction is a specific binding reaction. An embodiment of the present invention provides an optical system and a detection method. The optical system includes a first lens and a second lens which are arranged in sequence from the object side to the image side along the optical axis. The first lens and the second lens are arranged correspondingly. The second lens is configured in a mobile terminal. The first lens and the second lens have the same aperture number and the same focal length. The exit pupil of the first lens is located at the entrance pupil of the second lens. In this way, the mobile terminal can be used to collect image data of an external sample to be detected through the second lens and the first lens in sequence and obtain a corresponding optical image, so as to analyze and obtain a target base sequence, thereby making a gene sequencer more miniaturized and making gene sequencing more miniaturized, convenient and popular. BRIEF DESCRIPTION OF THE DRAWINGS In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work. FIG1 is a schematic diagram of a current gene sequencing method; FIG2 is a schematic diagram of the structure of an optical system provided in an embodiment of the present application; FIG3 is an optical principle diagram of an optical system provided in an embodiment of the present application; FIG4 is a schematic diagram of a detection process provided in an embodiment of the present application; FIG5 is a schematic diagram of the structure of a sample chip provided in an embodiment of the present application; FIG6 is a wavelength schematic diagram provided in an embodiment of the present application; FIG7 is a schematic diagram of field of view distribution in a sample chip provided in an embodiment of the present application; FIG8 is a parameter diagram provided in an embodiment of the present application; FIG. 9 is a schematic diagram of the structure of another sample chip provided in an embodiment of the present application. DETAILED DESCRIPTION In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the specific embodiments of the present invention are described in detail below with reference to the accompanying drawings. In the following description, many specific details are set forth to facilitate a full understanding of the present invention, but the present invention may also be implemented in other ways different from those described herein, and those skilled in the art may make similar generalizations without violating the connotation of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below. Secondly, the present invention is described in detail with reference to schematic diagrams. When describing the embodiments of the present invention in detail, for the sake of convenience, the cross-sectional diagrams showing the device structure will not be partially enlarged according to the general scale, and the schematic diagrams are only examples, which should not limit the scope of protection of the present invention. In addition, in actual production, the three-dimensional dimensions of length, width and depth should be included. As described in the background technology, in traditional gene sequencing methods, lasers are used to excite samples to produce fluorescence, which is then detected and analyzed by an imaging system consisting of a microscope and a camera to obtain the target base sequence. Currently, gene sequencing can only be completed by specific institutions such as hospitals, research institutes, and laboratories. This is because current gene sequencers are large in size, and even miniaturized, desktop gene sequencers require professional operators in specific environments to complete. Referring to FIG1, a schematic diagram of a current gene sequencing method is shown. The dotted line portion is the imaging system of the gene sequencer, which is composed of a microscope objective, a dichroic mirror, a filter, a tube lens, and a camera. The biological sample is combined with a fluorescent dye, and the sample is excited to generate a fluorescent signal through an illumination system including an excitation light source and a beam shaping module. The fluorescent signal generated by the sample is then photographed by a camera after passing through a microscope system composed of a microscope objective, a series of dichroic mirrors, a filter, and a tube lens. The target base sequence can be analyzed by analyzing the captured image. The imaging system of such a gene sequencer is large in size and complex in structure. The installation and use of the microscope objective requires professionals, which is not universal to the general public. In addition, the field of view of the microscope objective is small, and the requirements for the preparation of biological samples are relatively high. Based on this, an embodiment of the present application provides an optical system and a detection method, wherein the optical system includes a first lens and a second lens which are sequentially arranged along the optical axis from the object side toward the image side, the first lens and the second lens are arranged correspondingly, the second lens is configured in a mobile terminal, the first lens and the second lens have the same aperture number and the same focal length, and the exit pupil of the first lens is located at the entrance pupil of the second lens. In this way, the mobile terminal can be used to collect image data of an external sample to be detected through the second lens and the first lens in sequence and obtain a corresponding optical image, thereby analyzing and obtaining a target base sequence, making the gene sequencer more miniaturized, and making gene sequencing more miniaturized, convenient and popular. In order to better understand the technical solution and technical effects of the present application, specific embodiments will be described and illustrated in detail below with reference to the accompanying drawings. An optical system provided in an embodiment of the present application is shown in Figure 2, which is a structural schematic diagram of an optical system provided in an embodiment of the present application. The optical system includes a first lens 10 and a second lens 20 arranged in sequence from the object side to the image side along the optical axis direction. The first lens 10 and the second lens 20 are arranged correspondingly. The second lens 20 is configured in a mobile terminal 200 as a built-in lens of the mobile terminal 200. The first lens 10 serves as an additional lens of the mobile terminal. The first lens 10 and the second lens 20 have the same aperture number and the same focal length. The mobile terminal 200 can be a device with a camera such as a mobile phone or a tablet computer. The first lens 10 and the second lens 20 may have the same structure or different structures. Of course, different structures need to achieve the same aperture number and the same focal length. When the first lens 10 and the second lens 20 have the same structure, the first lens 10 and the second lens 20 are symmetrical about the center plane between the first lens 10 and the second lens 20. For example, the first lens 10 may be an inverted lens in another mobile terminal, or an independent lens. The first lens 10 and the second lens 20 may each include a plurality of lenses, that is, the first lens 10 includes a first optical element combination, the second lens 20 includes a second optical element combination, and the first optical element combination and the second optical element combination are arranged in mirror symmetry with each other. The optical element combination defined in the present application includes the surface structure of each optical element, the arrangement method, and the distance between the optical elements. Specifically, the exit pupil of the first lens 10 is located at the entrance pupil of the second lens 20, so that the light diffused by the first lens 10 can be completely received by the second lens 20 without causing signal loss, thereby achieving measurement of a large numerical aperture (NA) and a large field of view (FOV), so that the mobile terminal can be used to collect image data of an external sample to be detected through the second lens 20 and the first lens 10 in sequence and obtain a corresponding optical image. Let the aperture number of the first lens 10 and the second lens 20 be F, then the NA of the optical system is about 1 / 2F, the magnification β is 1, the image plane size is equal to the field of view size, the pixel size is α, the pixel size is X*Y, and the size of the FOV is α*(X+Y). For example, the aperture number of a certain mobile phone is F=1.6, the pixel size α=1.7um, and the pixel size is 3024*4032. The numerical aperture NA of the optical system is NA≈1 / 2F=0.3125, and the field of view size is FOV-X=6.8544mm and FOV-Y=5.1408mm. Referring to FIG3 , which is an optical principle diagram of an optical system provided by an embodiment of the present application, the structure in the left box is the structure of the first lens 10, and the structure in the right box is the structure of the second lens 20. In the direction from the first lens 10 to the second lens 20, the first lens 10 includes a first lens, a second lens, a third lens, a fourth lens and a fifth lens in sequence. The first lens is a plane mirror, the object side surface of the second lens is a concave surface, and the image side surface is a convex surface. The third lens and the fifth lens are both biconvex lenses, the object side surface of the fourth lens is a plane surface, and the image side surface is a concave surface. The second lens 20 is symmetrical to the first lens 10, and the second lens 20 includes a fifth lens, a fourth lens, a third lens, a second lens and a first lens in sequence. The two first lenses have the same structure and are symmetrical about the central plane, the two second lenses have the same structure and are symmetrical about the central plane, the two third lenses have the same structure and are symmetrical about the central plane, the two fourth lenses have the same structure and are symmetrical about the central plane, and the two fifth lenses have the same structure and are symmetrical about the central plane. In the embodiment of the present application, the number of the first lens 10 and the second lens 20 can be multiple, and the multiple first lenses 10 and the multiple second lenses 20 are arranged in pairs, that is, a corresponding first lens 10 and the first second lens 20 are regarded as a lens group. The optical system may include multiple lens groups. The first lens 10 and the second lens 20 belonging to the same lens group have the same focal length and the same aperture number, and the exit pupil of the first lens 10 is located at the entrance pupil of the second lens 20. Different first lenses 10 can have different focal planes, and these focal planes are parallel to each other. Specifically, multiple second lenses 20 can be set in different mobile terminals, so that the planes where the center points of the multiple second lenses 20 are located are perpendicular to the optical axis of the lens group. In this case, the second lenses 20 can have different focal lengths, so that the first lens 10 has different focal planes; or multiple second lenses 20 can also be set in different mobile terminals, and the first lens 10 can have different focal planes by setting the positions of the multiple mobile terminals and the focal lengths of the multiple second lenses 20. For example, the planes where the center points of the multiple second lenses 20 are located can be perpendicular to the optical axis of the lens group. In this case, the second lenses 20 can have different focal lengths, so that the first lens 10 has different focal planes. For example, the multiple second lenses 20 can be at different distances from the same focal plane, and the multiple second lenses 20 have the same focal length, so that the first lens 10 has different focal planes. As an example, the number of the first lens 10 and the second lens 20 is 2, the two second lenses 20 are arranged in the same mobile terminal and have different focal lengths, and the line connecting the center points of the two second lenses 20 is parallel to any focal plane, then the two second lenses 20 can respectively have two focal planes, which are recorded as the first focal plane and the second focal plane. By using the camera system and algorithm system of the mobile terminal, objects in the first focal plane and the second focal plane can be photographed, for example, focusing on the first focal plane to photograph a first object in the first focal plane, and then focusing on the second focal plane to photograph a second object in the second focal plane. In the embodiment of the present application, a filter 30 may be arranged between the corresponding first lens 10 and the second lens 20. The filter 30 may pass light of a preset band and block light of bands outside the preset band. The plane where the filter is located is the symmetrical center plane of the corresponding first lens 10 and the second lens 20. Based on an optical system provided in an embodiment of the present application, an embodiment of the present application also provides a detection method, in which a sample chip can generate a detectable fluorescence signal, and a first image can be obtained by photographing a first field of view area of ​​the sample chip 40 by connecting an additional lens to the mobile terminal 200. The mobile terminal 200 has a built-in lens, and the additional lens and the built-in lens have the same aperture number and the same focal length, and the exit pupil of the additional lens is located at the entrance pupil of the built-in lens. Specifically, before photographing the first field of view area, the excitation light source 50 can be used to illuminate the first field of view area of ​​the sample chip 40 from the side of the sample chip 40 away from the mobile terminal to excite a detectable fluorescent signal. The sample chip 40 has a sample to be detected, and the sample to be detected can be a biochemical sample to be detected, such as a DNA nanoball (DNB) combined with a fluorescent dye. The DNA nanoball combined with the fluorescent dye generates fluorescence when irradiated with the excitation light emitted by the excitation light source. In another embodiment of the present invention, an additional lens and a built-in lens are used to directly capture images of the fluorescence signal of the sample chip. In this case, there is no need to apply an excitation light source and a filter. The catalytic reaction in the sample chip produces a detectable fluorescence signal. For example, the detectable fluorescence signal in the sample chip is produced by enzyme-catalyzed substrates. The arrangement of the additional lens and the built-in lens can refer to the arrangement of the aforementioned first lens 10 and the second lens 20. Both the additional lens and the built-in lens can include multiple lenses, that is, the additional lens has a first optical element combination, the built-in lens has a second optical element combination, and the first optical element combination and the second optical element combination are arranged in mirror symmetry with each other. Referring to FIG. 4 , which is a schematic diagram of a detection process provided in an embodiment of the present application, the excitation light source 50 and the mobile terminal may be located on opposite sides of the sample chip 40, the side where the excitation light source 50 is located is recorded as the first side, and the side where the mobile terminal is located is recorded as the second side, and the additional lens is also located on the second side of the sample chip 40. When the excitation light source 50 is used to illuminate the first field of view area of ​​the sample chip 40 from the first side of the sample chip 40, the mobile terminal 200 may be used to photograph the first field of view area to obtain a first image. The size of the first field of view area is determined according to the field of view sizes of the additional lens and the built-in lens. The first image is formed on a sensor of the mobile terminal 200. The illumination of the first field of view area of ​​the sample chip 40 by the mobile terminal 200 may be achieved by the shooting system of the mobile terminal 200 itself. Referring to FIG5 , which is a schematic diagram of the structure of a sample chip provided in an embodiment of the present application, the sample chip 40 may include a substrate 401 and a cover plate 402, and the sample 403 to be tested is disposed between the substrate 401 and the cover plate 402. The sample 403 to be tested may be a nucleic acid library, wherein the cover plate 402 may be located on the side of the sample chip 40 facing the optical system, and the substrate 401 may be located on the side of the sample chip 40 facing the excitation light source 50. With the side where the substrate 401 is located as the bottom and the side where the cover plate 402 is located as the top, the nucleic acid library may be placed on the substrate 401 and then fixed by the top cover plate 402. The excitation light source 50 irradiates the nucleic acid library from the bottom, and the mobile terminal 200 is located above the sample chip 40 so that the mobile terminal 200 can shoot from above the sample chip 40. When a filter 30 is provided between the additional lens and the built-in lens, the filter 30 can be used to pass light of a preset wavelength band and suppress light of other wavelength bands outside the preset wavelength band. By selecting the filter, the selection of the preset wavelength band is realized, so that the central wavelength of the fluorescence excited by the sample chip 40 after being irradiated by the excitation light source 50 belongs to the preset wavelength band, and the excitation light emitted by the excitation light source 50 does not belong to the preset wavelength band, so that the selection of fluorescence and the filtering of excitation light can be realized by the filter 30. For example, the sample to be detected is a DNA nanosphere, which is combined with the fluorescent dye Alexa Fluor 532, and the central wavelength of its excitation light is 532nm, and the central wavelength of the emission light is 553nm. Then, an excitation light source with a central wavelength of 532nm and a filter with a preset wavelength band of 540nm to 580nm can be selected, as shown in Figure 6, which is a wavelength schematic diagram provided in an embodiment of the present application, wherein the horizontal axis is the wavelength and the vertical axis is dimensionless. It can be seen that the selection of fluorescence and the filtering of excitation light can be realized by the filter 30. The sample chip 40 may have a larger size so that more samples to be detected can be set. If the first field of view area is smaller than the entire area of ​​the sample chip 40, after photographing the first field of view area, the second field of view area of ​​the sample chip 40 may also be photographed. Specifically, if the detectable fluorescent signal is excited by the irradiation of the excitation light source, the excitation light source 50 may be turned off, and the sample chip 40 may be moved so that the built-in lens and the additional lens face the second field of view area of ​​the sample chip 40. Then, when the second field of view area of ​​the sample chip 40 is irradiated from the side of the sample chip 40 away from the mobile terminal by the excitation light source 50, the second field of view area is photographed by the mobile terminal 200 to obtain a second image; if the fluorescent signal is generated by a catalytic reaction in the sample chip, after photographing the first field of view area, the sample chip may be moved so that the built-in lens and the additional lens face the second field of view area of ​​the sample chip, and the second field of view area may be photographed by the mobile terminal to obtain a second image. The first field of view area and the second field of view area may have the same size. Of course, after photographing the second field of view area, the third field of view area and other areas of the sample chip 40 may also be photographed until all areas of the sample chip 40 are photographed. The target base sequence may be obtained by analyzing the photographed images. For the mobile phone with an aperture number F of 1.6 in the above example, the length sizes are FOV-X=6.8544mm, FOV-Y=5.1408mm. Assuming that the size of the sample chip 40 is 20mm*100mm, according to the size of the FOV, a chip has approximately 16 FOVs, as shown in Figure 7, which is a schematic diagram of the field of view distribution in a sample chip provided in an embodiment of the present application, and multiple FOVs are arranged vertically in the sample chip. If the fluorescence band is λ=553nm, according to the Rayleigh criterion, the interval between each DNB is at least σ=0.61λ / NA=1079nm, so that different DNBs can be accurately distinguished. Since the pixel size is α=1.7um and the magnification β=1, each DNB can occupy one pixel, and the distance between different DNBs can be one pixel. Then each DNB occupies at least 2 pixels in the horizontal direction and at least 2 pixels in the vertical direction, for a total of four pixels. Referring to FIG7 , the black circle represents the pixel. The size of each DNB in ​​the sample chip is Pitch=2*α / β=3400nm. The number of DNBs in each FOV is (3024*4032) / (2*2)=3048192. The total number of DNBs on a chip with a total of 16 FOVs is N=16*3048192=48.771M. That is to say, after 16 shots, the information of 48.771M samples can be completed. Generally speaking, there are four bases in each DNA. After the fluorescent dye binds to one base (e.g., base A) in the DNB in ​​the sample chip, the sample chip 40 can be photographed to obtain an image corresponding to the base. Assuming that the biochemical reaction time required for the fluorescent dye to bind to the base is 20 seconds, and the time required to photograph one FOV in the sample chip 40 is 10 seconds, the time required to obtain an image corresponding to one base is t=16*10s+20s=3min. The time required for the biochemical reaction and photographing of the four bases in the same sample chip 40 is t=16*10s+20s=3min. total =3min*4=12min, that is, 48.771M samples can be captured in 12 minutes. Referring to FIG8 , a parameter diagram is provided in an embodiment of the present application, wherein FIG8A indicates that the fluorescence wavelength (Longest dye wavelength) is 553 nm, the NA is 0.3125, the interval of each DNB obtained by the Rayleigh criterion (Resolution By Rayleigh) is 1079 nm, the size (Pitch) occupied by each DNB in ​​the sample chip is 3400 nm, the pixel size (Pixel Size) is 1.7 um, the ratio of pixel (pixel) to DNB is 2, the amplification (Amplification) is 1.00, the pixel size is: the X-direction size (Resolution-X) is 4032, the Y-direction size (Resolution-Y) is 3024, the diagonal size (Diagonal) in the mobile terminal sensor size (CMOS Sensor Size) is 8.57 mm, and the target FOV size (Objective The diagonal size of the FOV is 8.568 mm, the number of DNBs (DNB / FC) contained in each FOV is 3048192, the X-direction size of the target FOV (Objective FOV-X) is 6.8544 mm, the Y-direction size of the target FOV (Objective FOV-Y) is 5.1408 mm, the number of FOVs contained in each sample chip (FOV No / FC) is 16, the shooting time (Total Imaging Time) required for each sample chip is 12 min, and the number of samples contained in each sample chip (Spot Numbers / FC) is 48.771M. As can be seen from Figure 8B, the specifications of the sample chip (Chip Spec) include: FOV area (FOV Area) is 35.23709952 mm 2 The imaging time / chip is 3 min per round, the chip width is 20 mm, the chip length is 100 mm, and the chip area is 2000 mm. 2 . In an embodiment of the present application, in the optical system, the number of additional lenses and built-in lenses can be multiple, and the multiple additional lenses and the multiple built-in lenses are arranged corresponding to each other in pairs. Different additional lenses can have different focal planes, and the different focal planes are parallel to each other. In this way, the first field of view area may include multiple detection areas respectively located at the multiple focal planes, and the multiple detection areas are facing the multiple additional lenses, so that the multiple additional lenses and the built-in lenses corresponding to the multiple additional lenses are used to shoot the multiple detection areas, thereby further improving the shooting efficiency and reducing the number of times the sample chip is moved. Specifically, multiple built-in lenses can be arranged in different mobile terminals, so that the planes where the center points of the multiple built-in lenses are located are perpendicular to the optical axis of the lens group. In this case, the built-in lenses can have different focal lengths, so that the additional lenses have different focal planes; or multiple built-in lenses can also be arranged in different mobile terminals, and by setting the positions of multiple mobile terminals and the focal lengths of the multiple built-in lenses, the additional lenses can have different focal planes. For example, the planes where the center points of the multiple built-in lenses are located can be perpendicular to the optical axis of the lens group. In this case, the built-in lenses can have different focal lengths, so that the additional lenses have different focal planes. For another example, the multiple built-in lenses can be at different distances from the same focal plane, and the multiple built-in lenses have the same focal length, so that the additional lenses have different focal planes. As an example, the number of additional lenses and built-in lenses is 2, and the two built-in lenses are set in the same mobile terminal and have different focal lengths. The line connecting the center points of the two built-in lenses is parallel to any focal plane, and the two built-in lenses can have two focal planes respectively, which are recorded as the first focal plane and the second focal plane. Referring to FIG9, it is a structural schematic diagram of another sample chip provided in an embodiment of the present application. The sample chip includes a stacked substrate 401 and a cover plate 402, and two layers of samples to be tested 403 located between the substrate 401 and the cover plate 402. The two layers of samples to be tested 403 are located in the first focal plane and the second focal plane respectively. The first focal plane is the surface of the substrate 401 facing the cover plate 402, and the second focal plane is the surface of the cover plate 402 facing the substrate 401. In specific implementation, the mobile terminal can be used to focus on the first focal plane to shoot the sample to be tested at the first focal plane, and then focus on the second focal plane to shoot the sample to be tested at the second focal plane. Of course, the DNBs in the two layers of samples to be tested can be staggered to avoid mutual influence during shooting. The spacing between adjacent focal planes in the plurality of focal planes can enable one of the focal planes to be located within the focal depth range of other additional lenses other than the corresponding additional lens. For example, the spacing between adjacent focal planes is 1 um to 10 um. The embodiment of the present invention provides a detection method, in which a first image is obtained by shooting a first field of view area of ​​a sample chip with an additional lens connected to an external mobile terminal, the mobile terminal has a built-in lens, the additional lens and the built-in lens have the same aperture number and the same focal length, the exit pupil of the additional lens is located at the entrance pupil of the built-in lens, the mobile terminal and the additional lens are located on one side of the sample chip, and a detectable fluorescent signal can be generated in the sample chip. In this way, when the sample chip is irradiated with a laser light source, the sample chip can be photographed with a mobile terminal, so as to analyze and obtain the target base sequence, making the gene sequencer more miniaturized, and making gene sequencing more miniaturized, convenient and popular. The embodiment of the present application also provides a portable biochemical inspection optical system, including a mobile terminal and an auxiliary mechanism cooperating with the mobile terminal. The relationship between the mobile terminal and the auxiliary mechanism is equivalent to that between the mobile terminal 200 and the additional lens mentioned above. The mobile terminal has a second lens, the auxiliary mechanism is detachably mounted on the mobile terminal, and the auxiliary mechanism includes a first lens, the first lens corresponds to the second lens and is mounted on the object side of the second lens, the first lens and the second lens have the same aperture number and the same focal length, and the exit pupil of the first lens is located at the entrance pupil of the second lens; the mobile terminal is used to collect detectable signals of an external biochemical sample to be detected through the second lens and the first lens in sequence and obtain a corresponding optical image. Optionally, the first lens has a first optical element combination, the second lens has a second optical element combination, and the first optical element combination and the second optical element combination are arranged in mirror symmetry with each other. Optionally, the auxiliary mechanism includes a filter located on a side of the second lens close to the first lens, and the second optical element combination and the second optical element combination are arranged in mirror symmetry with respect to the filter. Optionally, the biochemical sample to be detected is a nucleic acid sequencing library. Optionally, the biochemical sample to be tested is a tissue sample. Optionally, the biochemical sample to be detected undergoes a biochemical reaction with reagents of multiple different reaction components to generate the detectable signal; the reaction components include at least one of a sample generation component or a sample analysis component. Optionally, the biochemical reaction includes controlling reaction conditions to allow different sample generating components to generate the sample; and the biochemical reaction includes analyzing the sample, including allowing sample analyzing components to react with the sample to provide the associated detectable signal. An embodiment of the present application also provides a biochemical testing method, comprising: loading a sample to be tested into a channel of a chip circulation pool; and loading a reagent having a plurality of different reaction components into the channel of the chip circulation pool to perform a biochemical reaction of the sample to be tested and the reagent; the reaction component comprises at least one of a sample generation component or a sample analysis component. Optionally, the biochemical reaction includes generating a sample in a channel of the chip circulation pool, including allowing different sample generating components to flow into the channel and controlling the reaction conditions of the channel to generate the sample; and the biochemical reaction includes analyzing the sample in the channel, including allowing sample analysis components to flow into the channel, and the sample analysis components react with the sample to provide a relevant detectable signal. The biochemical testing method also includes: using the above-mentioned portable optical system to identify the detectable signal, the portable optical system includes a mobile terminal and an auxiliary mechanism cooperating with the mobile terminal, the mobile terminal has a second lens, the auxiliary mechanism is detachably installed on the mobile terminal, and the auxiliary mechanism includes a first lens, the first lens corresponds to the second lens and is installed on the object side of the second lens, the first lens and the second lens have the same aperture number and the same focal length, and the exit pupil of the first lens is located at the entrance pupil of the second lens; the mobile terminal collects the detectable signal through the second lens and the first lens in turn and obtains the corresponding optical image. Optionally, the biochemical reaction is a nucleic acid sequencing reaction, and the sample to be detected is a nucleic acid sequencing library. Optionally, the detectable signal is an optical signal. Optionally, the sample to be detected is a tissue sample, and the biochemical reaction is a specific binding reaction. Each embodiment in this specification is described in a progressive manner, and the same or similar parts between the embodiments can be referred to each other, and each embodiment focuses on the differences from other embodiments. In particular, for the memory device embodiment, since it is basically similar to the method embodiment, the description is relatively simple, and the relevant parts can be referred to the partial description of the method embodiment. The above is only a preferred embodiment of the present invention. Although the present invention has been disclosed as a preferred embodiment, it is not intended to limit the present invention. Any technician familiar with the art can make many possible changes and modifications to the technical solution of the present invention by using the above disclosed methods and technical contents without departing from the scope of the technical solution of the present invention, or modify it into an equivalent embodiment of equivalent changes. Therefore, any simple modification, equivalent change and modification made to the above embodiments according to the technical essence of the present invention without departing from the content of the technical solution of the present invention still falls within the scope of protection of the technical solution of the present invention.

Claims

The biochemical sample undergoes a biochemical reaction with a plurality of reagents of different reaction components to generate the detectable signal; The reaction components include at least one of a sample generation component or a sample analysis component; Optionally, the biochemical reaction includes controlling reaction conditions so that different sample generating components generate the sample; and The biochemical reaction includes analyzing the sample, including allowing sample analysis components to react with the sample to provide the associated detectable signal. A biochemical test method, characterized in that: include: Loading the sample to be tested into the channel of the chip flow cell; and loading reagents having a plurality of different reaction components into the channels of the chip flow cell to perform a biochemical reaction between the sample to be detected and the reagents; The reaction components include at least one of a sample generation component or a sample analysis component; Optionally, the biochemical reaction comprises generating a sample in a channel of the chip flow cell, comprising allowing different sample generating components to flow into the channel and controlling reaction conditions of the channel to generate the sample; and The biochemical reaction includes analyzing the sample in the channel, including flowing a sample analysis component into the channel, the sample analysis component reacting with the sample to provide an associated detectable signal; The biochemical testing method also includes: using a portable optical system to identify the detectable signal, the portable optical system includes a mobile terminal and an auxiliary mechanism cooperating with the mobile terminal, the mobile terminal has a second lens, the auxiliary mechanism is detachably installed on the mobile terminal, and the auxiliary mechanism includes a first lens, the first lens corresponds to the second lens and is installed on the object side of the second lens, the first lens and the second lens have the same aperture number and the same focal length, and the exit pupil of the first lens is located at the entrance pupil of the second lens; the mobile terminal collects the detectable signal through the second lens and the first lens in turn and obtains the corresponding optical image. The biochemical testing method according to claim 20, characterized in that The biochemical reaction is a nucleic acid sequencing reaction, and the sample to be detected is a nucleic acid sequencing library. The biochemical testing method according to claim 20, characterized in that The detectable signal is an optical signal. The biochemical testing method according to claim 20, characterized in that The sample to be detected is a tissue sample, and the biochemical reaction is a specific binding reaction.

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