Large-scale Epinephelus oanceolutus high-yield culture method

Abstract

The invention discloses a large-scale Epinephelus oanceolutus high-yield breeding method which comprises the following steps: (1) breeding environment setting: selecting an indoor breeding pond with the breeding density of 500-1000 Epinephelus oanceolutus per mu, and adjusting water quality parameters; (2) pretreatment before fry throwing: soaking Epinephelus oanceolutus fries in a mixed liquid medicine formed by mixing a citric acid aqueous solution, a feather cockscomb extracting solution and an elsholtzia extracting solution, and throwing the soaked fries into an indoor culture pond; (3) performing sterilization management after seedling throwing: uniformly splashing sterilization powder to the whole pond once every 14-21 days after throwing, wherein the sterilization powder comprises agrimony extract, catalpa extract and pomegranate rind extract; (4) performing illumination management after seedling throwing: keeping an alternating state of illumination and darkness after throwing; light sources for illumination are green light, white light and yellow light; and (5) performing bait management after fry casting. The adult Epinephelus oanceolutus cultured through the steps is high in disease resistance, high in survival rate and high in yield.

Description

Technical Field

[0001] The present invention relates to the technical field of aquaculture, and particularly relates to a high-yield aquaculture method for large-scale pearl gentian grouper. Background Art

[0002] Grouper is an important aquaculture species in seawater aquaculture. Pearl gentian, also known as pearl grouper or tiger-lion grouper, is a new variety bred by crossing Epinephelus fuscoguttatus♀ with Epinephelus lanceolatus♂. Pearl gentian combines the characteristics of fast growth of the male parent and delicate meat of the female parent, and has the appearance of a tiger grouper head and a giant grouper tail. It can be cultured as an ornamental fish and is known as "the rising star of grouper". Pearl gentian has strong adaptability and is suitable for various aquaculture methods such as net cages and ponds. However, with the expansion of the aquaculture scale, it is found that disease problems occur continuously and the growth rate is slow when culturing pearl gentian grouper with the existing aquaculture methods, which seriously restricts the development of the aquaculture industry. Summary of the Invention

[0003] In view of the above-mentioned defects of the prior art, the present invention provides a high-yield aquaculture method for large-scale pearl gentian grouper to solve the problems raised in the above background art.

[0004] To achieve the above object, the present invention provides the following technical solutions:

[0005] A high-yield aquaculture method for large-scale pearl gentian grouper, comprising the following steps:

[0006] (1) Setting of aquaculture environment

[0007] Select an indoor aquaculture pond, with a stocking density of 500 - 1000 tails / mu, and adjust the water quality parameters;

[0008] (2) Pretreatment before fry stocking

[0009] Select healthy pearl gentian grouper fry, soak them in a mixed medicinal solution composed of 35 - 40 mg / L citric acid aqueous solution, 200 - 400 mg / L extract of Celosia argentea L., and 300 - 450 mg / L extract of Elsholtzia ciliata (Thunb.) Hyland. for 3 - 6 minutes, and then put the soaked fry into the indoor aquaculture pond;

[0010] (3) Sterilization management after fry stocking

[0011] After stocking, sprinkle bactericidal powder evenly throughout the pond every 14 - 21 days. The bactericidal powder includes Agrimonia pilosa Ledeb. (the whole herb of the Rosaceae plant Agrimonia pilosa Ledeb.) extract, Lithospermum zollingeri A.DC. extract, and pomegranate peel (the dried pericarp of the Punica granatum L. of the Punicaceae plant) extract in a weight ratio of 3 - 10:1:5 - 15;

[0012] (4) Light management after stocking

[0013] When culturing Epinephelus lanceolatus fries after stocking, keep them in an alternating state of light irradiation and darkness; the light sources for light irradiation are green light, white light, and yellow light;

[0014] (5) Feed management after stocking

[0015] Alternately feed nutritional feed and growth feed, with the daily feeding amount being 4 - 15% of the weight of Epinephelus lanceolatus. First, feed nutritional feed for 30 - 45 days, then feed growth feed for 15 - 25 days, and alternate feeding until adult fish; the nutritional feed is prepared by adding licochalcone A to basic feed A, and the growth feed is prepared by adding daphnetin to basic feed B.

[0016] Preferably, the water quality parameters in step (1) are water salinity of 22 - 28‰, pH of 6.0 - 6.5, dissolved oxygen of 6 - 8 mg / L, water temperature of 23 - 26°C, nitrite content of 0.050 - 0.080 mg / L, and ammonia nitrogen content of 0.1 - 0.2 mg / L.

[0017] Preferably, the preparation method of the Celosia argentea extract in step (2) is: crush the stems, leaves or roots of Celosia argentea, add 10 - 15 times the weight of water of Celosia argentea and decoct for 30 - 60 minutes, then filter to obtain the filtrate, which is the Celosia argentea extract.

[0018] Preferably, the preparation method of the Elsholtzia ciliata extract in step (2) is: crush Elsholtzia ciliata, add 50 - 60 times the weight of water of Elsholtzia ciliata, first boil over high heat, then simmer for 40 - 45 minutes, and filter to obtain the filtrate, which is the Elsholtzia ciliata extract.

[0019] Preferably, the dosage of the bactericidal powder in step (3) is 5 - 10 kg / mu each time.

[0020] Preferably, the time ratio of light irradiation to darkness in step (4) is 3:1.

[0021] Preferably, the time of light irradiation and darkness in step (4) is darkness from 0 - 6 o'clock, green light irradiation from 6 - 12 o'clock, white light irradiation from 12 - 18 o'clock, and green light irradiation from 18 - 24 o'clock.

[0022] Preferably, the wavelength range of the green light in step (4) is 520±5 nm, the light intensity is 400-800 Lux, the wavelength range of the white light is 450±5 nm, the light intensity is 1000-1200 Lux, and the wavelength range of the yellow light is 590±5 nm, and the light intensity is 500-700 Lux.

[0023] Preferably, the basic feed A in step (5) comprises, by weight, 15-25 parts of corn flour, 10-20 parts of soybean meal, 10-15 parts of bone meal, and 5-12 parts of seaweed powder; the basic feed B comprises, by weight, 30-45 parts of silkworm pupa powder, 30-40 parts of peanut meal, 22-30 parts of rice distiller's grains, 18-25 parts of sweet potato leaves, and 1-3 parts of table salt.

[0024] Preferably, the addition amounts of licorice chalcone A and daphnetin in the nutritional bait and the growth bait in step (5) are 8-12 wt%.

[0025] Compared with the prior art, the beneficial effects of the present invention are as follows: the present invention provides a large-scale high-yield breeding method for Epinephelus lanceolatus, and before the fry are put in, they are soaked in a mixed medicinal liquid composed of a citric acid aqueous solution, a Celosia argentea extract, and an Elsholtzia ciliata extract, which can effectively kill the germs carried by the fry and improve their disease resistance. Regularly spraying bactericidal powder can effectively control the growth of germs in the water body. The main components of the bactericidal powder are all from plant traditional Chinese medicine components, which are safe and efficient and will not have side effects on the fry; by setting the alternating state of light irradiation and darkness, selecting the light sources for light irradiation as green light, white light, and yellow light and setting the irradiation time, the growth rate of Epinephelus lanceolatus is significantly improved, and the ability to resist diseases is improved; by setting the method of alternately feeding nutritional bait and growth bait and selecting appropriate bait components, it is convenient for digestion, absorption, and conversion, enhances the immunity of Epinephelus lanceolatus, and adding natural products licorice chalcone A and daphnetin to the basic feed A and the basic feed B has an obvious positive effect on their survival rate and yield. Detailed implementation manners

[0026] The technical solutions of the present invention will be further described in detail below in conjunction with the specific implementation manners.

[0027] Example 1

[0028] A large-scale high-yield breeding method for Epinephelus lanceolatus includes the following steps:

[0029] (1) Setting of the breeding environment

[0030] Select an indoor breeding pond with a breeding density of 500 tails / mu, and adjust the water quality parameters to a water salinity of 22‰, a pH of 6.0, a dissolved oxygen of 6 mg / L, a water temperature of 23°C, a nitrite content of 0.050 mg / L, and an ammonia nitrogen content of 0.1 mg / L;

[0031] (2) Pre-treatment before seeding

[0032] Select healthy Epinephelus lanceolatus fries, soak them in a mixed medicinal solution composed of 35 mg / L citric acid aqueous solution, 200 mg / L Celosia argentea extract, and 300 mg / L Mosla chinensis extract for 3 minutes, and then put the soaked fries into an indoor culture pond; the preparation method of the Celosia argentea extract is: take the stems, leaves or roots of Celosia argentea, crush them, add 10 times the weight of water of Celosia argentea and decoct for 30 minutes, filter to obtain the filtrate, which is the Celosia argentea extract; the preparation method of the Mosla chinensis extract is: take Mosla chinensis, crush it, add 50 times the weight of water of Mosla chinensis, first boil it over high heat, then simmer for 40 minutes, filter to obtain the filtrate, which is the Mosla chinensis extract;

[0033] (3) Post-seeding sterilization management

[0034] After seeding, sprinkle a sterilizing powder evenly throughout the pond every 14 days, and the dosage of the sterilizing powder each time is 5 kg / mu. The sterilizing powder includes Agrimonia pilosa extract, Lithospermum erythrorhizon extract, and Pomegranate peel extract with a weight ratio of 3:1:5;

[0035] (4) Post-seeding light management

[0036] After seeding, keep the Epinephelus lanceolatus fries in an alternating state of light irradiation and darkness during cultivation, and the time ratio of light irradiation to darkness is 3:1; the light sources for light irradiation are green light, white light, and yellow light; the time of light irradiation and darkness is darkness from 0 to 6 o'clock, green light irradiation from 6 to 12 o'clock, white light irradiation from 12 to 18 o'clock, and green light irradiation from 18 to 24 o'clock; the wavelength range of the green light is 520 ± 5 nm, the light intensity is 400 Lux, the wavelength range of the white light is 450 ± 5 nm, the light intensity is 1000 Lux, the wavelength range of the yellow light is 590 ± 5 nm, and the light intensity is 500 Lux;

[0037] (5) Post-seeding bait management

[0038] Alternately feed nutritional bait and growth bait, and the daily feeding amount is 4% of the weight of Epinephelus lanceolatus. First, feed the nutritional bait for 30 days, then feed the growth bait for 15 days, and alternate feeding until they grow into adult fish; the nutritional bait is prepared by adding licochalcone A to basic feed A, and the growth bait is prepared by adding daphnetin to basic feed B; the basic feed A includes, by weight, 15 parts of corn flour, 10 parts of soybean meal, 10 parts of bone meal, and 5 parts of seaweed powder; the basic feed B includes, by weight, 30 parts of silkworm pupa powder, 30 parts of peanut meal, 22 parts of rice wine lees, 18 parts of sweet potato leaves, and 1 part of salt; the addition amounts of licochalcone A and daphnetin in the nutritional bait and growth bait are 8 wt%, respectively.

[0039] Example 2

[0040] A large-scale high-yield aquaculture method for pearl spotted groupers, comprising the following steps:

[0041] (1) Setting up the aquaculture environment

[0042] Select an indoor aquaculture pond with a stocking density of 1000 tails per mu, and adjust the water quality parameters to a water salinity of 28‰, pH 6.5, dissolved oxygen of 8 mg / L, water temperature of 26 °C, nitrite content of 0.080 mg / L, and ammonia nitrogen content of 0.2 mg / L;

[0043] (2) Pretreatment before stocking

[0044] Select healthy pearl spotted grouper fry, soak them in a mixed medicinal solution composed of 40 mg / L citric acid aqueous solution, 400 mg / L celosia argentea extract, and 450 mg / L elsholtzia ciliata extract for 6 minutes, and then put the soaked fry into the indoor aquaculture pond; the preparation method of the celosia argentea extract is as follows: take the stems, leaves or roots of celosia argentea, crush them, add 15 times the weight of water of celosia argentea, decoct for 60 minutes, filter, and obtain the filtrate, which is the celosia argentea extract; the preparation method of the elsholtzia ciliata extract is as follows: take elsholtzia ciliata, crush it, add 60 times the weight of water of elsholtzia ciliata, first boil it over high heat, then simmer for 45 minutes, filter, and obtain the filtrate, which is the elsholtzia ciliata extract;

[0045] (3) Sterilization management after stocking

[0046] After stocking, sprinkle a sterilizing powder evenly throughout the pond every 21 days, and the dosage of the sterilizing powder each time is 10 kg per mu. The sterilizing powder includes agrimony extract, lithospermum erythrorhizon extract, and pomegranate peel extract with a weight ratio of 10:1:15;

[0047] (4) Lighting management after stocking

[0048] After stocking, when raising pearl spotted grouper fry, keep them in an alternating state of light irradiation and darkness, and the time ratio of light irradiation to darkness is 3:1; the light sources for light irradiation are green light, white light, and yellow light; the time of light irradiation and darkness is darkness from 0 to 6 o'clock, green light irradiation from 6 to 12 o'clock, white light irradiation from 12 to 18 o'clock, and green light irradiation from 18 to 24 o'clock; the wavelength range of the green light is 520 ± 5 nm, the light intensity is 800 Lux, the wavelength range of the white light is 450 ± 5 nm, the light intensity is 1200 Lux, the wavelength range of the yellow light is 590 ± 5 nm, and the light intensity is 700 Lux;

[0049] (5) Feed management after stocking

[0050] Feed nutritious bait and growth bait alternately, with the daily feeding amount being 15% of the weight of the pearl grouper. First, feed the nutritious bait for 45 days, then feed the growth bait for 25 days, and alternate feeding until the fish grow into adults. The nutritious bait is prepared by adding liquiritigenin A to the basic feed A, and the growth bait is prepared by adding daphnetin to the basic feed B. The basic feed A includes, by weight, 25 parts of corn flour, 20 parts of soybean meal, 15 parts of bone meal, and 12 parts of seaweed powder. The basic feed B includes, by weight, 45 parts of silkworm chrysalis powder, 40 parts of peanut meal, 30 parts of rice distiller's grains, 25 parts of sweet potato leaves, and 3 parts of table salt. The addition amounts of liquiritigenin A and daphnetin in the nutritious bait and growth bait are 12 wt%, respectively.

[0051] Example 3

[0052] A large-scale high-yield breeding method for pearl groupers includes the following steps:

[0053] (1) Setting up the breeding environment

[0054] Select an indoor breeding pond with a breeding density of 800 fish per mu. Adjust the water quality parameters to a water salinity of 25‰, pH 6.2, dissolved oxygen of 7 mg / L, water temperature of 25°C, nitrite content of 0.070 mg / L, and ammonia nitrogen content of 0.15 mg / L.

[0055] (2) Pretreatment before fry stocking

[0056] Select healthy pearl grouper fry, soak them in a mixed medicinal solution composed of 36 mg / L citric acid aqueous solution, 300 mg / L celosia argentea extract, and 400 mg / L elsholtzia ciliata extract for 5 minutes, and then put the soaked fry into the indoor breeding pond. The preparation method of the celosia argentea extract is as follows: crush the stems, leaves or roots of celosia argentea, add 12 times the weight of water of celosia argentea, decoct for 50 minutes, and filter to obtain the filtrate, which is the celosia argentea extract. The preparation method of the elsholtzia ciliata extract is as follows: crush elsholtzia ciliata, add 55 times the weight of water of elsholtzia ciliata, first boil over high heat, then simmer for 42 minutes, and filter to obtain the filtrate, which is the elsholtzia ciliata extract.

[0057] (3) Sterilization management after fry stocking

[0058] After fry stocking, sprinkle a bactericidal powder evenly throughout the pond every 18 days. The dosage of the bactericidal powder each time is 8 kg per mu. The bactericidal powder includes agrimony extract, lithospermum erythrorhizon extract, and pomegranate peel extract in a weight ratio of 7:1:10.

[0059] (4) Lighting management after fry stocking

[0060] After stocking the pearl grouper fry, maintain the farming environment in an alternating state of light exposure and darkness, with the time ratio of light exposure to darkness being 3:1. The light sources for light exposure are green light, white light, and yellow light. The time for light exposure and darkness is as follows: darkness from 0 to 6 hours, green light exposure from 6 to 12 hours, white light exposure from 12 to 18 hours, and green light exposure from 18 to 24 hours. The wavelength range of the green light is 520 ± 5 nm, and the light intensity is 600 Lux. The wavelength range of the white light is 450 ± 5 nm, and the light intensity is 1100 Lux. The wavelength range of the yellow light is 590 ± 5 nm, and the light intensity is 600 Lux.

[0061] (5) Feed management after stocking

[0062] Alternately feed nutritional feed and growth feed, with the daily feeding amount being 10% of the body weight of the pearl grouper. First, feed the nutritional feed for 40 days, then feed the growth feed for 20 days, and alternate feeding until the fish grow into adults. The nutritional feed is prepared by adding licorice chalcone A to basic feed A, and the growth feed is prepared by adding daphnetin to basic feed B. The basic feed A, by weight, includes 20 parts of corn flour, 18 parts of soybean meal, 12 parts of bone meal, and 10 parts of seaweed powder. The basic feed B, by weight, includes 40 parts of silkworm chrysalis powder, 35 parts of peanut meal, 25 parts of rice distiller's grains, 20 parts of sweet potato leaves, and 2 parts of table salt. The addition amounts of licorice chalcone A and daphnetin in the nutritional feed and growth feed are both 10 wt%.

[0063] Comparative Example 1

[0064] The difference between this comparative example and Example 3 is that the fry were not soaked in the mixed medicinal liquid, and the other components and steps remained unchanged.

[0065] A large-scale high-yield farming method for pearl groupers includes the following steps:

[0066] (1) Setting up the farming environment

[0067] Select an indoor farming pond with a farming density of 800 tails per mu, and adjust the water quality parameters to a water salinity of 25‰, pH 6.2, dissolved oxygen of 7 mg / L, water temperature of 25°C, nitrite content of 0.070 mg / L, and ammonia nitrogen content of 0.15 mg / L.

[0068] (2) Sterilization management after stocking

[0069] After stocking, evenly sprinkle a sterilizing powder all over the pond every 18 days. The amount of the sterilizing powder used each time is 8 kg per mu. The sterilizing powder includes Agrimony extract, Lithospermum extract, and Pomegranate peel extract with a weight ratio of 7:1:10.

[0070] (3) Light management after stocking

[0071] After stocking the fries, during the cultivation of Epinephelus lanceolatus fries, maintain an alternating state of light irradiation and darkness, with the time ratio of light irradiation to darkness being 3:1; the light sources for light irradiation are green light, white light, and yellow light; the time for light irradiation and darkness is 0 - 6 hours for darkness, 6 - 12 hours for green light irradiation, 12 - 18 hours for white light irradiation, and 18 - 24 hours for green light irradiation; the wavelength range of the green light is 520 ± 5 nm, the light intensity is 600 Lux, the wavelength range of the white light is 450 ± 5 nm, the light intensity is 1100 Lux, the wavelength range of the yellow light is 590 ± 5 nm, and the light intensity is 600 Lux;

[0072] (4) Feed management after stocking

[0073] Alternately feed nutritional feed and growth feed, with the daily feeding amount being 10% of the weight of Epinephelus lanceolatus, first feed the nutritional feed for 40 days, then feed the growth feed for 20 days, and alternate feeding until the fish grow into adults; the nutritional feed is prepared by adding licochalcone A to basic feed A, and the growth feed is prepared by adding daphnetin to basic feed B; the basic feed A includes, by weight, 20 parts of corn flour, 18 parts of soybean meal, 12 parts of bone meal, and 10 parts of seaweed powder; the basic feed B includes, by weight, 40 parts of silkworm chrysalis powder, 35 parts of peanut meal, 25 parts of rice distiller's grains, 20 parts of sweet potato leaves, and 2 parts of table salt; the addition amounts of licochalcone A and daphnetin in the nutritional feed and growth feed are 10 wt%, respectively.

[0074] Comparative Example 2

[0075] The difference between this comparative example and Example 3 is that the mixed liquid medicine is adjusted to 5% potassium permanganate, and the other components and steps remain unchanged.

[0076] A large - scale high - yield cultivation method for Epinephelus lanceolatus includes the following steps:

[0077] (1) Cultivation environment setting

[0078] Select an indoor cultivation pond with a cultivation density of 800 fries per mu, and adjust the water quality parameters to a water salinity of 25‰, pH 6.2, dissolved oxygen of 7 mg / L, water temperature of 25°C, nitrite content of 0.070 mg / L, and ammonia nitrogen content of 0.15 mg / L;

[0079] (2) Pretreatment before stocking

[0080] Select healthy Epinephelus lanceolatus fries, soak them in 5% potassium permanganate for 5 minutes, and then put the soaked fries into the indoor cultivation pond;

[0081] (3) Sterilization management after stocking

[0082] After stocking the fry, sprinkle bactericidal powder evenly throughout the pond every 18 days. The dosage of bactericidal powder each time is 8 kg / mu. The bactericidal powder includes Agrimonia pilosa Ledeb. extract, Lithospermum erythrorhizon Sieb. extract and Granatum pericarpium extract in a weight ratio of 7:1:10;

[0083] (4) Light management after stocking the fry

[0084] When culturing Epinephelus lanceolatus fries, keep them in an alternating state of light irradiation and darkness after stocking. The time ratio of light irradiation to darkness is 3:1; the light sources for light irradiation are green light, white light and yellow light; the time of light irradiation and darkness are darkness from 0 to 6 o'clock, green light irradiation from 6 to 12 o'clock, white light irradiation from 12 to 18 o'clock, and green light irradiation from 18 to 24 o'clock; the wavelength range of the green light is 520±5 nm, the light intensity is 600 Lux, the wavelength range of the white light is 450±5 nm, the light intensity is 1100 Lux, the wavelength range of the yellow light is 590±5 nm, and the light intensity is 600 Lux;

[0085] (5) Feed management after stocking the fry

[0086] Alternately feed nutritional feed and growth feed. The daily feeding amount is 10% of the weight of Epinephelus lanceolatus. First, feed nutritional feed for 40 days, then feed growth feed for 20 days, and alternate feeding until adulthood; the nutritional feed is prepared by adding licochalcone A to basic feed A, and the growth feed is prepared by adding daphnetin to basic feed B; the basic feed A includes, by weight, 20 parts of corn flour, 18 parts of soybean meal, 12 parts of bone meal, and 10 parts of seaweed powder; the basic feed B includes, by weight, 40 parts of silkworm chrysalis powder, 35 parts of peanut meal, 25 parts of rice distiller's grains, 20 parts of sweet potato leaves, and 2 parts of table salt; the addition amounts of licochalcone A and daphnetin in the nutritional feed and growth feed are 10 wt%, respectively.

[0087] Comparative Example 3

[0088] The difference between this comparative example and Example 3 is that the mixed liquid medicine is adjusted to 20 mg / L povidone iodine, and the other components and steps remain unchanged.

[0089] A large-scale high-yield aquaculture method for Epinephelus lanceolatus, comprising the following steps:

[0090] (1) Setting of aquaculture environment

[0091] Select an indoor aquaculture pond with a stocking density of 800 tails / mu, and adjust the water quality parameters to a water salinity of 25‰, pH 6.2, dissolved oxygen of 7 mg / L, water temperature of 25°C, nitrite content of 0.070 mg / L, and ammonia nitrogen content of 0.15 mg / L;

[0092] (2) Pretreatment before stocking the fry

[0093] Select healthy pearl grouper fry, soak them in 20 mg / L povidone iodine for 5 minutes, and then put the soaked fry into an indoor breeding pond.

[0094] (3) Disinfection management after fry stocking

[0095] After fry stocking, sprinkle bactericidal powder evenly throughout the pond every 18 days. The dosage of bactericidal powder each time is 8 kg / mu. The bactericidal powder includes Agrimony extract, Lithospermum erythrorhizon extract and Pomegranate peel extract with a weight ratio of 7:1:10.

[0096] (4) Lighting management after fry stocking

[0097] After fry stocking, keep the pearl grouper fry in an alternating state of light irradiation and darkness. The time ratio of light irradiation to darkness is 3:1. The light sources for light irradiation are green light, white light and yellow light. The time for light irradiation and darkness is as follows: darkness from 0 to 6 o'clock, green light irradiation from 6 to 12 o'clock, white light irradiation from 12 to 18 o'clock, and green light irradiation from 18 to 24 o'clock. The wavelength range of the green light is 520±5 nm, and the light intensity is 600 Lux. The wavelength range of the white light is 450±5 nm, and the light intensity is 1100 Lux. The wavelength range of the yellow light is 590±5 nm, and the light intensity is 600 Lux.

[0098] (5) Feeding management after fry stocking

[0099] Alternately feed nutritional bait and growth bait. The daily feeding amount is 10% of the weight of the pearl grouper. First, feed nutritional bait for 40 days, then feed growth bait for 20 days, and alternate feeding until the fish grow into adults. The nutritional bait is prepared by adding liquiritigenin A to basic feed A, and the growth bait is prepared by adding daphnetin to basic feed B. The basic feed A includes, by weight, 20 parts of corn flour, 18 parts of soybean meal, 12 parts of bone meal, and 10 parts of seaweed powder. The basic feed B includes, by weight, 40 parts of silkworm chrysalis powder, 35 parts of peanut meal, 25 parts of rice wine lees, 20 parts of sweet potato leaves, and 2 parts of salt. The addition amounts of liquiritigenin A and daphnetin in the nutritional bait and growth bait are 10 wt%, respectively.

[0100] Comparative Example 4

[0101] The difference between this comparative example and Example 3 is that the bactericidal powder is adjusted to chlorine dioxide disinfectant, and the other components and steps remain unchanged.

[0102] A large-scale high-yield breeding method for pearl grouper, comprising the following steps:

[0103] (1) Setting of breeding environment

[0104] Select an indoor aquaculture pond with a stocking density of 800 tails per mu, and adjust the water quality parameters to a water salinity of 25‰, pH 6.2, dissolved oxygen of 7 mg / L, water temperature of 25 °C, nitrite content of 0.070 mg / L, and ammonia nitrogen content of 0.15 mg / L;

[0105] (2) Pretreatment before stocking

[0106] Select healthy Epinephelus fuscoguttatus fries, soak them in a mixed medicinal solution composed of 36 mg / L citric acid aqueous solution, 300 mg / L Celosia argentea extract, and 400 mg / L Mosla chinensis extract for 5 minutes, and then put the soaked fries into the indoor aquaculture pond; the preparation method of the Celosia argentea extract is: take the stems, leaves or roots of Celosia argentea, crush them, add 12 times the weight of water of Celosia argentea and decoct for 50 minutes, filter to obtain the filtrate, which is the Celosia argentea extract; the preparation method of the Mosla chinensis extract is: take Mosla chinensis, crush it, add 55 times the weight of water of Mosla chinensis, first boil it over high heat, then simmer for 42 minutes, filter to obtain the filtrate, which is the Mosla chinensis extract;

[0107] (3) Disinfection management after stocking

[0108] After stocking, evenly sprinkle chlorine dioxide disinfectant throughout the pond once every 18 days, and the concentration of chlorine dioxide disinfectant is 50 mg / L;

[0109] (4) Lighting management after stocking

[0110] After stocking, keep the Epinephelus fuscoguttatus fries in an alternating state of light irradiation and darkness, and the time ratio of light irradiation to darkness is 3:1; the light sources for light irradiation are green light, white light, and yellow light; the time of light irradiation and darkness are darkness from 0 to 6 o'clock, green light irradiation from 6 to 12 o'clock, white light irradiation from 12 to 18 o'clock, and green light irradiation from 18 to 24 o'clock; the wavelength range of the green light is 520 ± 5 nm, the light intensity is 600 Lux, the wavelength range of the white light is 450 ± 5 nm, the light intensity is 1100 Lux, the wavelength range of the yellow light is 590 ± 5 nm, and the light intensity is 600 Lux;

[0111] (5) Feed management after stocking

[0112] Alternately feed nutritional bait and growth bait, with the daily feeding amount being 10% of the weight of the pearl grouper. First, feed the nutritional bait for 40 days, then feed the growth bait for 20 days, and alternate feeding until the fish grow into adults. The nutritional bait is prepared by adding liquiritigenin A to basic feed A, and the growth bait is prepared by adding daphnetin to basic feed B. The basic feed A includes, by weight, 20 parts of corn flour, 18 parts of soybean meal, 12 parts of bone meal, and 10 parts of seaweed powder. The basic feed B includes, by weight, 40 parts of silkworm chrysalis powder, 35 parts of peanut meal, 25 parts of rice distillers' grains, 20 parts of sweet potato leaves, and 2 parts of table salt. The addition amounts of liquiritigenin A and daphnetin in the nutritional bait and growth bait are 10 wt%, respectively.

[0113] Comparative Example 5

[0114] The difference between this comparative example and Example 3 is that: lithospermum extract is not added to the bactericidal powder, and the other components and steps remain unchanged.

[0115] A large-scale high-yield cultivation method for pearl groupers includes the following steps:

[0116] (1) Setting of the cultivation environment

[0117] Select an indoor cultivation pond with a cultivation density of 800 fish per mu, and adjust the water quality parameters to a water salinity of 25‰, pH 6.2, dissolved oxygen of 7 mg / L, water temperature of 25 °C, nitrite content of 0.070 mg / L, and ammonia nitrogen content of 0.15 mg / L.

[0118] (2) Pretreatment before fry stocking

[0119] Select healthy pearl grouper fry, soak them in a mixed medicinal solution composed of 36 mg / L citric acid aqueous solution, 300 mg / L celosia argentea extract, and 400 mg / L elsholtzia ciliata extract for 5 minutes, and then put the soaked fry into the indoor cultivation pond. The preparation method of the celosia argentea extract is as follows: crush the stems, leaves or roots of celosia argentea, add 12 times the weight of water of celosia argentea and decoct for 50 minutes, then filter to obtain the filtrate, which is the celosia argentea extract. The preparation method of the elsholtzia ciliata extract is as follows: crush elsholtzia ciliata, add 55 times the weight of water of elsholtzia ciliata, first boil over high heat, then simmer for 42 minutes, and filter to obtain the filtrate, which is the elsholtzia ciliata extract.

[0120] (3) Bactericidal management after fry stocking

[0121] Sprinkle the bactericidal powder evenly throughout the pond once every 18 days after fry stocking. The dosage of the bactericidal powder each time is 8 kg per mu. The bactericidal powder includes agrimony extract and pomegranate peel extract with a weight ratio of 7:10.

[0122] (4) Lighting management after fry stocking

[0123] After stocking the juvenile Epinephelus lanceolatus, maintain the culture environment in an alternating state of light exposure and darkness, with the time ratio of light exposure to darkness being 3:1. The light sources for light exposure are green light, white light, and yellow light. The time for light exposure and darkness is as follows: darkness from 0 to 6 o'clock, green light exposure from 6 to 12 o'clock, white light exposure from 12 to 18 o'clock, and green light exposure from 18 to 24 o'clock. The wavelength range of the green light is 520 ± 5 nm, and the light intensity is 600 Lux; the wavelength range of the white light is 450 ± 5 nm, and the light intensity is 1100 Lux; the wavelength range of the yellow light is 590 ± 5 nm, and the light intensity is 600 Lux.

[0124] (5) Feed management after stocking

[0125] Alternately feed nutritional feed and growth feed, with the daily feeding amount being 10% of the weight of Epinephelus lanceolatus. First, feed the nutritional feed for 40 days, then feed the growth feed for 20 days, and alternate feeding until the fish grow into adults. The nutritional feed is prepared by adding licochalcone A to basic feed A, and the growth feed is prepared by adding daphnetin to basic feed B. The basic feed A, by weight, includes 20 parts of corn flour, 18 parts of soybean meal, 12 parts of bone meal, and 10 parts of seaweed powder. The basic feed B, by weight, includes 40 parts of silkworm pupa powder, 35 parts of peanut meal, 25 parts of rice distiller's grains, 20 parts of sweet potato leaves, and 2 parts of table salt. The addition amounts of licochalcone A and daphnetin in the nutritional feed and growth feed are both 10 wt%.

[0126] Comparative Example 6

[0127] The difference between this comparative example and Example 3 is that in step (4), the light source for light exposure is white light, and the other components and steps remain unchanged.

[0128] A large-scale high-yield cultivation method for Epinephelus lanceolatus includes the following steps:

[0129] (1) Setting of the cultivation environment

[0130] Select an indoor cultivation pond with a cultivation density of 800 fish per mu, and adjust the water quality parameters to a water salinity of 25‰, pH 6.2, dissolved oxygen of 7 mg / L, water temperature of 25°C, nitrite content of 0.070 mg / L, and ammonia nitrogen content of 0.15 mg / L.

[0131] (2) Pretreatment before stocking

[0132] Select healthy pearl grouper fry and soak them in a mixed medicinal solution composed of 36 mg / L citric acid aqueous solution, 300 mg / L celosia argentea extract, and 400 mg / L elsholtzia ciliata extract for 5 minutes. Then, release the soaked fry into an indoor breeding pond. The preparation method of the celosia argentea extract is as follows: crush the stems, leaves, or roots of celosia argentea, add 12 times the weight of water of celosia argentea, decoct for 50 minutes, and filter to obtain the filtrate, which is the celosia argentea extract. The preparation method of the elsholtzia ciliata extract is as follows: crush elsholtzia ciliata, add 55 times the weight of water of elsholtzia ciliata, first boil over high heat, then simmer for 42 minutes, and filter to obtain the filtrate, which is the elsholtzia ciliata extract.

[0133] (3) Disinfection management after fry release

[0134] After fry release, sprinkle a disinfectant powder evenly throughout the pond every 18 days. The dosage of the disinfectant powder each time is 8 kg / mu. The disinfectant powder includes Agrimonia pilosa extract, Lithospermum erythrorhizon extract, and Punica granatum peel extract with a weight ratio of 7:1:10.

[0135] (4) Lighting management after fry release

[0136] After fry release, keep the pearl grouper fry in an alternating state of light irradiation and darkness during cultivation. The time ratio of light irradiation to darkness is 3:1. The light source for light irradiation is white light. The time of light irradiation and darkness is 0 - 6 hours for darkness and 6 - 24 hours for white light irradiation respectively. The wavelength range of the white light is 450 ± 5 nm, and the light intensity is 1100 Lux.

[0137] (5) Feeding management after fry release

[0138] Alternately feed nutritional bait and growth bait. The daily feeding amount is 10% of the weight of the pearl grouper. First, feed the nutritional bait for 40 days, then feed the growth bait for 20 days, and alternate feeding until the fish grow into adults. The nutritional bait is prepared by adding licochalcone A to basic feed A, and the growth bait is prepared by adding daphnetin to basic feed B. The basic feed A, by weight, includes 20 parts of corn flour, 18 parts of soybean meal, 12 parts of bone meal, and 10 parts of seaweed powder. The basic feed B, by weight, includes 40 parts of silkworm chrysalis powder, 35 parts of peanut meal, 25 parts of rice distiller's grains, 20 parts of sweet potato leaves, and 2 parts of table salt. The addition amounts of licochalcone A and daphnetin in the nutritional bait and growth bait are 10 wt%, respectively.

[0139] Comparative Example 7

[0140] The difference between this comparative example and Example 3 is that in step (4), the time ratio of light irradiation to darkness is 7:5 (i.e., 14L:10D), and the other components and steps remain unchanged.

[0141] A large-scale high-yield breeding method for pearl grouper includes the following steps:

[0142] (1) Aquaculture environment setting

[0143] Select an indoor aquaculture pond with a stocking density of 800 tails per mu, and adjust the water quality parameters to a water salinity of 25‰, pH 6.2, dissolved oxygen of 7 mg / L, water temperature of 25°C, nitrite content of 0.070 mg / L, and ammonia nitrogen content of 0.15 mg / L;

[0144] (2) Pretreatment before fry stocking

[0145] Select healthy pearl grouper fry, soak them in a mixed medicinal liquid composed of 36 mg / L citric acid aqueous solution, 300 mg / L celosia argentea extract, and 400 mg / L elsholtzia ciliata extract for 5 minutes, and then put the soaked fry into the indoor aquaculture pond; the preparation method of the celosia argentea extract is: crush the stems, leaves or roots of celosia argentea, add 12 times the weight of water of celosia argentea and decoct for 50 minutes, filter to obtain the filtrate, which is the celosia argentea extract; the preparation method of the elsholtzia ciliata extract is: crush elsholtzia ciliata, add 55 times the weight of water of elsholtzia ciliata, first boil over high heat, then simmer for 42 minutes, filter to obtain the filtrate, which is the elsholtzia ciliata extract;

[0146] (3) Disinfection management after fry stocking

[0147] After fry stocking, sprinkle the disinfection powder evenly throughout the pond every 18 days. The dosage of the disinfection powder each time is 8 kg per mu. The disinfection powder includes Agrimonia pilosa extract, Lithospermum erythrorhizon extract, and Pomegranate peel extract in a weight ratio of 7:1:10;

[0148] (4) Lighting management after fry stocking

[0149] After fry stocking, keep the pearl grouper fry in an alternating state of light irradiation and darkness. The time ratio of light irradiation to darkness is 7:5; the light sources for light irradiation are green light, white light, and yellow light; the time of light irradiation and darkness is 20 - 06 hours for darkness, 6 - 11 hours for green light irradiation, 11 - 16 hours for white light irradiation, and 16 - 20 hours for green light irradiation; the wavelength range of the green light is 520 ± 5 nm, the light intensity is 600 Lux, the wavelength range of the white light is 450 ± 5 nm, the light intensity is 1100 Lux, the wavelength range of the yellow light is 590 ± 5 nm, and the light intensity is 600 Lux;

[0150] (5) Feed management after fry stocking

[0151] Feeding nutritious bait and growth bait alternately, with a daily feeding amount of 10% of the body weight of pearl grouper, first feeding the nutritious bait for 40 days, then feeding the growth bait for 20 days, and feeding alternately until the fish grow up; the nutritious bait is prepared by adding licorice chalcone A to basic feed A, and the growth bait is prepared by adding daphneline to basic feed B; the basic feed A includes 20 parts of corn flour, 18 parts of soybean meal, 12 parts of bone meal, and 10 parts of seaweed powder by weight; the basic feed B includes 40 parts of silkworm pupa powder, 35 parts of peanut meal, 25 parts of rice wine lees, 20 parts of sweet potato leaves, and 2 parts of salt by weight; the added amounts of licorice chalcone A and daphneline in the nutritious bait and the growth bait are 10wt% respectively.

[0152] Comparative Example 8

[0153] The difference between this comparative example and Example 3 is that the light source for illumination in step (4) is adjusted to blue, and the other components and steps remain unchanged.

[0154] A large-scale high-yield breeding method for pearl grouper comprises the following steps:

[0155] (1) Breeding environment setting

[0156] Select indoor breeding ponds with a breeding density of 800 per mu, and adjust the water quality parameters to 25‰ salinity, pH 6.2, 7mg / L dissolved oxygen, 25℃ water temperature, 0.070mg / L nitrite, and 0.15mg / L ammonia nitrogen.

[0157] (2) Pretreatment before seedling planting

[0158] Select healthy pearl grouper fry, place them in a mixed solution of 36 mg / L citric acid aqueous solution, 300 mg / L Celosia australis extract and 400 mg / L Elsholtzia extract for immersion for 5 minutes, and then put the fry into an indoor breeding pond after immersion; the preparation method of the Celosia australis extract is: take the stems, leaves or roots of Celosia australis, crush them, add water 12 times the weight of Celosia australis to boil for 50 minutes, filter, and obtain a filtrate, which is the Celosia australis extract; the preparation method of the Elsholtzia extract is: take the Elsholtzia australis and crush it, add water 55 times the weight of Elsholtzia australis, boil it over high heat, then simmer it over low heat for 42 minutes, filter, and obtain a filtrate, which is the Elsholtzia australis extract;

[0159] (3) Sterilization management after seedling planting

[0160] After the seedlings are planted, the whole pond is evenly sprayed with sterilizing powder every 18 days, and the amount of sterilizing powder used each time is 8kg / mu. The sterilizing powder includes a spatholobi extract, a catalpa extract and a pomegranate peel extract in a weight ratio of 7:1:10;

[0161] (4) Light management after seedling planting

[0162] After stocking the fry, during the cultivation of Epinephelus lanceolatus fries, maintain an alternating state of light irradiation and darkness, with the time ratio of light irradiation to darkness being 3:1; the light source for light irradiation is blue light; the time for light irradiation and darkness is 0-6 hours for darkness and 6-14 hours for blue light irradiation respectively; the wavelength range of the blue light is 450 nm, and the light intensity is 1100 Lux;

[0163] (5) Feed management after stocking

[0164] Alternately feed nutritional feed and growth feed, with the daily feeding amount being 10% of the weight of Epinephelus lanceolatus, first feed the nutritional feed for 40 days, then feed the growth feed for 20 days, and alternate feeding until it becomes an adult fish; the nutritional feed is prepared by adding licochalcone A to basic feed A, and the growth feed is prepared by adding daphnetin to basic feed B; the basic feed A includes, by weight, 20 parts of corn flour, 18 parts of soybean meal, 12 parts of bone meal, and 10 parts of seaweed powder; the basic feed B includes, by weight, 40 parts of silkworm chrysalis powder, 35 parts of peanut meal, 25 parts of rice distillers' grains, 20 parts of sweet potato leaves, and 2 parts of table salt; the addition amounts of licochalcone A and daphnetin in the nutritional feed and growth feed are 10 wt% respectively.

[0165] Comparative Example 9

[0166] The difference between this comparative example and Example 3 is that licochalcone A is not added to the nutritional feed, and the other components and steps remain unchanged.

[0167] A large-scale high-yield cultivation method for Epinephelus lanceolatus includes the following steps:

[0168] (1) Cultivation environment setting

[0169] Select an indoor cultivation pond, with a cultivation density of 800 tails per mu, and adjust the water quality parameters to a water salinity of 25‰, pH 6.2, dissolved oxygen of 7 mg / L, water temperature of 25 °C, nitrite content of 0.070 mg / L, and ammonia nitrogen content of 0.15 mg / L;

[0170] (2) Pretreatment before stocking

[0171] Select healthy Epinephelus lanceolatus fries, soak them in a mixed medicinal liquid composed of 36 mg / L citric acid aqueous solution, 300 mg / L Celosia argentea extract, and 400 mg / L Elsholtzia ciliata extract for 5 minutes, and then put the soaked fries into the indoor cultivation pond; the preparation method of the Celosia argentea extract is: take the stems, leaves or roots of Celosia argentea, crush them, add 12 times the weight of water of Celosia argentea, decoct for 50 minutes, filter, and obtain the filtrate, which is the Celosia argentea extract; the preparation method of the Elsholtzia ciliata extract is: take Elsholtzia ciliata, crush it, add 55 times the weight of water of Elsholtzia ciliata, first boil it over high heat, then simmer it over low heat for 42 minutes, filter, and obtain the filtrate, which is the Elsholtzia ciliata extract;

[0172] (3) Post-seeding sterilization management

[0173] After seeding, sprinkle bactericidal powder evenly throughout the pond every 18 days. The dosage of bactericidal powder each time is 8 kg / mu. The bactericidal powder includes Agrimonia pilosa extract, Lithospermum erythrorhizon extract and pomegranate peel extract in a weight ratio of 7:1:10;

[0174] (4) Post-seeding lighting management

[0175] When culturing Epinephelus lanceolatus fries after seeding, keep them in an alternating state of light irradiation and darkness. The time ratio of light irradiation to darkness is 3:1; the light sources for light irradiation are green light, white light and yellow light; the time of light irradiation and darkness is darkness from 0 to 6 o'clock, green light irradiation from 6 to 12 o'clock, white light irradiation from 12 to 18 o'clock, and green light irradiation from 18 to 24 o'clock; the wavelength range of the green light is 520±5 nm, the light intensity is 600 Lux, the wavelength range of the white light is 450±5 nm, the light intensity is 1100 Lux, the wavelength range of the yellow light is 590±5 nm, and the light intensity is 600 Lux;

[0176] (5) Post-seeding bait management

[0177] Alternately feed nutritional bait and growth bait. The daily feeding amount is 10% of the weight of Epinephelus lanceolatus. First, feed nutritional bait for 40 days, then feed growth bait for 20 days, and alternate feeding until adult fish; the growth bait is prepared by adding daphnetin to basic feed B; the basic feed A includes, by weight, 20 parts of corn flour, 18 parts of soybean meal, 12 parts of bone meal, and 10 parts of seaweed powder; the basic feed B includes, by weight, 40 parts of silkworm chrysalis powder, 35 parts of peanut meal, 25 parts of rice distiller's grains, 20 parts of sweet potato leaves, and 2 parts of salt; the addition amount of daphnetin in the growth bait is 10 wt%.

[0178] Comparative Example 10

[0179] The difference between this comparative example and Example 3 is that daphnetin is not added to the growth bait, and the other components and steps remain unchanged.

[0180] A large-scale high-yield aquaculture method for Epinephelus lanceolatus includes the following steps:

[0181] (1) Aquaculture environment setting

[0182] Select an indoor aquaculture pond with a stocking density of 800 tails / mu, and adjust the water quality parameters to a water salinity of 25‰, pH 6.2, dissolved oxygen of 7 mg / L, water temperature of 25°C, nitrite content of 0.070 mg / L, and ammonia nitrogen content of 0.15 mg / L;

[0183] (2) Pre-seeding pretreatment

[0184] Select healthy Epinephelus lanceolatus fry, soak them in a mixed medicinal liquid composed of 36 mg / L citric acid aqueous solution, 300 mg / L Celosia argentea extract and 400 mg / L Elsholtzia ciliata extract for 5 min, and then put the soaked fry into an indoor breeding pond; the preparation method of the Celosia argentea extract is as follows: take the stems, leaves or roots of Celosia argentea, crush them, add 12 times the weight of water of Celosia argentea and decoct for 50 min, filter to obtain the filtrate, which is the Celosia argentea extract; the preparation method of the Elsholtzia ciliata extract is as follows: take Elsholtzia ciliata, crush it, add 55 times the weight of water of Elsholtzia ciliata, first boil it over high heat, then simmer for 42 min, filter to obtain the filtrate, which is the Elsholtzia ciliata extract;

[0185] (3) Post-seeding sterilization management

[0186] After seeding, sprinkle a sterilizing powder evenly over the whole pond every 18 days, and the dosage of the sterilizing powder each time is 8 kg / mu. The sterilizing powder includes Agrimonia pilosa extract, Lithospermum erythrorhizon extract and Punica granatum peel extract with a weight ratio of 7:1:10;

[0187] (4) Post-seeding lighting management

[0188] After seeding, keep the Epinephelus lanceolatus fry in an alternating state of light irradiation and darkness, and the time ratio of light irradiation to darkness is 3:1; the light source for light irradiation is green light, white light and yellow light; the time of light irradiation and darkness is darkness from 0 to 6 o'clock, green light irradiation from 6 to 12 o'clock, white light irradiation from 12 to 18 o'clock, and green light irradiation from 18 to 24 o'clock; the wavelength range of the green light is 520±5 nm, the light intensity is 600 Lux, the wavelength range of the white light is 450±5 nm, the light intensity is 1100 Lux, the wavelength range of the yellow light is 590±5 nm, and the light intensity is 600 Lux;

[0189] (5) Post-seeding bait management

[0190] Alternately feed nutritional bait and growth bait, and the daily feeding amount is 10% of the weight of Epinephelus lanceolatus. First feed the nutritional bait for 40 days, then feed the growth bait for 20 days, and alternately feed until they grow into adult fish; the nutritional bait is prepared by adding licochalcone A to the basic feed A; the basic feed A includes, by weight, 20 parts of corn flour, 18 parts of soybean meal, 12 parts of bone meal, and 10 parts of seaweed powder; the basic feed B includes, by weight, 40 parts of silkworm chrysalis powder, 35 parts of peanut meal, 25 parts of rice distiller's grains, 20 parts of sweet potato leaves, and 2 parts of table salt; the addition amount of licochalcone A in the growth bait is 10 wt%.

[0191] Test example

[0192] The situation of pearl grouper Epinephelus lanceolatus cultured for 5 months using the culture methods of Examples 1-3 and Comparative Examples 1-10 of the present invention was counted. The results are shown in Table 1. The enteritis of grouper mainly shows symptoms such as black body color, bloated belly, and white feces. It is highly contagious and has a relatively high mortality rate, especially common in the seedling stage.

[0193] Table 1 Culture situation of pearl grouper Epinephelus lanceolatus

[0194]

[0195] As can be seen from Table 1, using the culture methods of Examples 1-3 can reduce the disease rates of enteritis, gill rot, black body disease, and tail rot during the culture process, with a high survival rate. The average weight of each pearl grouper Epinephelus lanceolatus reaches 3.2 catties or more, and it can meet the market requirements after 5 months of culture, greatly saving the culture cost and bringing huge economic benefits. This culture method is suitable for large-scale culture and promotion. By comparing Example 3 with Comparative Examples 1-3, it can be seen that pearl grouper Epinephelus lanceolatus needs to be soaked and disinfected before being put into the culture pond to kill the harmful bacteria carried on the fish body. Potassium permanganate and povidone iodine are commonly used chemical bactericides, but their side effects will affect the survival rate of fry. Soaking with a Chinese medicine-based liquid medicine is safer and more efficient in killing bacteria and has no other side effects; by comparing Example 3 with Comparative Examples 4 and 5, it can be seen that the bactericidal powder obtained by mixing natural plant active ingredients can regulate the water environment, purify the water quality, increase the biological immunity of pearl grouper Epinephelus lanceolatus, and effectively resist the invasion of foreign pathogenic microorganisms such as bacteria and viruses; by comparing Example 3 with Comparative Examples 6-8, it can be seen that choosing the appropriate color and time of the light source significantly affects the growth of fish. Through multiple experiments in this application, it is confirmed that the light sources for light irradiation are green light, white light, and yellow light, and the ratio of the light irradiation time to the dark time is set. By precisely controlling the light conditions, the survival rate of pearl grouper Epinephelus lanceolatus can be effectively improved; by comparing Example 3 with Comparative Examples 9 and 10, it can be seen that alternately feeding nutritional bait and growth bait and adding a certain amount of natural products, licorice chalcone A and daphnetin, to the bait can effectively improve the feeding rate of fish, enhance the body immunity, and rapidly increase the growth rate of pearl grouper Epinephelus lanceolatus.

[0196] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A large-scale high-yield breeding method for pearl grouper, characterized in that: The following steps are involved: (1) Breeding environment setting Choose an indoor breeding pond with a breeding density of 500 to 1,000 per mu and adjust the water quality parameters; (2) Pretreatment before seedling planting Select healthy pearl grouper fry, soak them in a mixed solution of 35-40 mg / L citric acid aqueous solution, 200-400 mg / L Amaranthus australis extract and 300-450 mg / L Elsholtzia ciliata extract for 3-6 minutes, and then put the soaked fry into an indoor breeding pond; (3) Sterilization management after seedling planting After the seedlings are planted, the whole pond is evenly sprayed with sterilizing powder every 14 to 21 days, wherein the sterilizing powder comprises an extract of Agrimonia oleracea, an extract of Catalpa ovata and an extract of pomegranate peel in a weight ratio of 3 to 10:1:5 to 15; (4) Light management after seedling planting After the seedlings are put in, the pearl grouper fry are kept in an alternating state of light irradiation and darkness during cultivation; the light source of the light irradiation is green light, white light and yellow light; (5) Feed management after seedling release Nutritive feed and growth feed were fed alternately, with a daily feeding amount of 4-15% of the body weight of pearl grouper. The fish were first fed with nutritious feed for 30-45 days, and then fed with growth feed for 15-25 days, and the feeding was repeated alternately until the fish became adults. The nutritious feed was prepared by adding licorice chalcone A to basic feed A, and the growth feed was prepared by adding daphneline to basic feed B.

2. The large-scale high-yield culture method of pearl grouper according to claim 1, characterized in that: The water quality parameters of step (1) are water salinity 22-28‰, pH 6.0-6.5, dissolved oxygen 6-8 mg / L, water temperature 23-26°C, nitrite content 0.050-0.080 mg / L, and ammonia nitrogen content 0.1-0.2 mg / L.

3. The large-scale high-yield culture method of pearl grouper according to claim 1, characterized in that: The preparation method of the C. acutissima extract in step (2) is as follows: the stems, leaves or roots of C. acutissima are crushed, 10 to 15 times the weight of water of C. acutissima is added and boiled for 30 to 60 minutes, and the filtrate is filtered to obtain the C. acutissima extract.

4. The large-scale high-yield culture method of pearl grouper according to claim 1 is characterized in that: The preparation method of the Elsholtzia extract in step (2) is as follows: grind the Elsholtzia, add 50 to 60 times the weight of water as the Elsholtzia, boil over high heat, simmer over low heat for 40 to 45 minutes, filter, and obtain a filtrate, which is the Elsholtzia extract.

5. The large-scale high-yield culture method of pearl grouper according to claim 1 is characterized in that: The amount of the bactericidal powder used in step (3) is 5 to 10 kg / mu each time.

6. The large-scale high-yield culture method of pearl grouper according to claim 1 is characterized in that: In step (4), the ratio of light exposure to darkness is 3:

1.

7. The large-scale high-yield culture method of pearl grouper according to claim 6 is characterized in that: In step (4), the light irradiation and dark time are respectively 0-6 hours for darkness, 6-12 hours for green light irradiation, 12-18 hours for white light irradiation, and 18-24 hours for green light irradiation.

8. The large-scale high-yield culture method of pearl grouper according to claim 1 is characterized in that: In step (4), the wavelength range of the green light is 520±5nm, and the illumination intensity is 400-800Lux; the wavelength range of the white light is 450±5nm, and the illumination intensity is 1000-1200Lux; the wavelength range of the yellow light is 590±5nm, and the illumination intensity is 500-700Lux.

9. The large-scale high-yield culture method of pearl grouper according to claim 1, characterized in that: The basic A feed in step (5) comprises, by weight, 15 to 25 parts of corn flour, 10 to 20 parts of soybean meal, 10 to 15 parts of bone meal, and 5 to 12 parts of seaweed powder; the basic B feed comprises, by weight, 30 to 45 parts of silkworm pupa powder, 30 to 40 parts of peanut meal, 22 to 30 parts of rice wine lees, 18 to 25 parts of sweet potato leaves, and 1 to 3 parts of salt.

10. The large-scale high-yield breeding method of pearl grouper according to claim 1, characterized in that: In step (5), the amount of licorice chalcone A and daphnetoside added to the nutritional feed and the growth feed respectively is 8-12wt%.

Images