Flumetralin synergistic composition, pesticide and application of flumetralin synergistic composition and pesticide

Abstract

The invention relates to a flumetralin synergistic composition, a pesticide and application of the flumetralin synergistic composition and the pesticide, and belongs to the technical field of plant growth regulators. Experiments prove that when octanoic acid, 1-aminocyclopropane carboxylic acid or itaconic acid and flumetralin are combined for use, the effect of the composition is obviously better than that of flumetralin used alone, and the drug effect lasts for a longer time. It is proved for the first time that caprylic acid, 1-aminocyclopropane carboxylic acid or itaconic acid and flumetralin have a synergistic effect, the pesticide effect can be better achieved, meanwhile, the use amount of flumetralin can be remarkably reduced, and the problems that the yield of agricultural products is reduced and the quality of the agricultural products is reduced due to improper inhibition or phytotoxicity caused by single use of flumetralin can be better solved.

Description

Technical Field

[0001] The present invention relates to a flumetralin synergistic composition, a pesticide and its application, and belongs to the technical field of plant growth regulators. Background Art

[0002] Flumetralin, with the chemical name of N-(2-chloro-6-fluorobenzyl)-N-ethyl-2,6-dinitro-4-trifluoromethylaniline, is a contact and locally systemic plant growth regulator that can affect the function of enzyme systems in plants. Flumetralin is absorbed through the stems and leaves of plants and has a local conduction function. It inhibits the division and growth of meristematic cells at the contact site, thus having the effect of inhibiting the growth of tender buds and new shoots. Flumetralin mainly acts on lateral buds and has no phytotoxicity to fully expanded leaves. In the field of agricultural technology, it is widely used in tobacco bud inhibition, cotton pruning and shaping without topping, citrus summer shoot control, etc.

[0003] Flumetralin belongs to dinitroaniline pesticides and also has herbicidal activity. Its effect is easily affected by field conditions, plant growth and application techniques, etc., and there may be phenomena such as an unclear and inconsistent growth inhibition effect. Moreover, excessive or unreasonable use may cause phytotoxicity similar to the side effects of herbicides, such as curled and deformed young leaves, tissue chlorosis with patches or even necrosis. Therefore, the application of flumetralin in plant growth regulation has great limitations, with characteristics such as strict requirements for the application period, precise dosage control, and narrow application range. Currently, for the use of flumetralin, a compounding method is usually adopted to reduce the dosage and improve the drug effect.

[0004] The Chinese patent application for invention with the publication date of April 21, 2020 and the publication number of CN111034728A discloses a tobacco axillary bud inhibitor and a tobacco axillary bud inhibition method, specifically discloses flumetralin and prohexadione-calcium as active ingredients, and the combination of flumetralin and prohexadione-calcium shows a synergistic effect in inhibiting tobacco axillary buds, and can inhibit the germination and growth of tobacco axillary buds for a long time at a low application dose.

[0005] The Chinese invention patent with the announcement date of March 25, 2022 and the announcement number of CN113068691B discloses a special synergistic composition of axillary bud inhibitor containing uniconazole, specifically discloses that azone and Astragalus complanatus extract synergize with flumetralin and uniconazole compositions for bud inhibition on tobacco and cotton, effectively improving the bud inhibition effect and the bud inhibition duration of the compound preparation of uniconazole and flumetralin. The bud inhibition rate gradually decreases from 90.71 - 97.93% at 2 weeks after application to 83.59 - 91.25% at 6 weeks after application.

[0006] The Chinese invention patent with the publication date of July 25, 2023 and the publication number of CN115316385B discloses a pesticide composition containing paclobutrazol and flumetralin and its applications, specifically discloses that the paclobutrazol and flumetralin composition is used for controlling the shoot growth of citrus, and the shoot control rate can be increased from 44 - 64.89% of using flumetralin alone to 76.44 - 88.44%.

[0007] The above patents all control the potential phytotoxicity risk and high-dose residue risk of flumetralin to a certain extent through the way of combined synergism. However, in current agricultural production, there are still technical requirements for continuous improvement and optimization in aspects such as the efficacy, residue, and phytotoxicity of using flumetralin, and it is hoped to further improve in aspects such as reducing the dosage, increasing the quick-acting property, extending the residual period, increasing the use safety, broadening the use range, and reducing the agricultural production cost. Summary of the Invention

[0008] The first object of the present invention is to provide a flumetralin synergistic composition to solve the problems of excessive use times and short residual period existing in the composition mainly composed of flumetralin in the prior art.

[0009] The second object of the present invention is to provide a pesticide including the flumetralin synergistic composition to solve the problems of poor efficacy and low safety existing in the pesticide mainly composed of flumetralin as the main active ingredient in the prior art.

[0010] The third object of the present invention is to provide an application of a pesticide including the flumetralin synergistic composition in plant growth regulation, and provide an application of a pesticide with flumetralin as the active ingredient in plant growth regulation.

[0011] In order to achieve the above objects, the technical solution of a composition containing flumetralin in the present invention is as follows:

[0012] A flumetralin synergistic composition, the composition includes flumetralin and organic acid; the mass ratio of flumetralin to organic acid is (10 - 40):(1 - 40); the organic acid is one of octanoic acid, 1-aminocyclopropanecarboxylic acid, and itaconic acid.

[0013] The beneficial effects of the above technical solution are as follows: The flumetralin synergistic composition of the present invention belongs to a pioneering invention. The present invention proves through experiments that when using octanoic acid, 1-aminocyclopropanecarboxylic acid or itaconic acid in combination with flumetralin, the effect is significantly better than that of using flumetralin alone, and the duration of the efficacy is longer. The present invention first proves that there is a synergistic effect between octanoic acid, 1-aminocyclopropanecarboxylic acid or itaconic acid and flumetralin. While better achieving the efficacy, it can significantly reduce the usage amount of flumetralin, and can better solve the problems of improper inhibition or phytotoxicity when using flumetralin alone, resulting in reduced agricultural product yield and quality decline.

[0014] As a further improvement, the mass ratio of flumetralin to octanoic acid is (10 - 40):(10 - 25).

[0015] As a further improvement, the mass ratio of flumetralin to 1-aminocyclopropanecarboxylic acid is (10 - 40):(1 - 10).

[0016] As a further improvement, the mass ratio of flumetralin to itaconic acid is (10 - 40):(25 - 40).

[0017] To achieve the above object, the technical solution of a pesticide including a flumetralin synergistic composition in the present invention is:

[0018] A pesticide including a flumetralin synergistic composition, wherein the mass percentage of the flumetralin synergistic composition in the pesticide is 11 - 80%.

[0019] The beneficial effects of the above technical solution are as follows: Through field efficacy experiments, it is proved that there is a synergistic effect between flumetralin and organic acids such as octanoic acid, 1-aminocyclopropanecarboxylic acid or itaconic acid. It can be prepared into a pesticide with flumetralin as the main active ingredient to achieve reduced use. The pesticide including the flumetralin synergistic composition in the present invention has the advantages of less application amount and frequency, quick effect, long-lasting effect, and good safety compared with the commonly used flumetralin suspension concentrate and flumetralin emulsifiable concentrate at present. It can achieve the effects of reduced use, being healthier for crops, and being more environmentally friendly, and has great economic and social benefits.

[0020] As a further improvement, the dosage form of the pesticide is a suspension concentrate, a soluble concentrate or an emulsifiable concentrate.

[0021] According to actual application requirements, the pesticide including the flumetralin synergistic composition in the present invention is prepared into one of dosage forms such as a water emulsion, a microemulsion, a suspension concentrate, a soluble concentrate, an emulsifiable concentrate, etc. by adding a wetting and dispersing agent, an emulsifier, a penetrant, a pH regulator, an antifreeze, a thickener, a dispersion medium, etc. according to the methods well-known to those skilled in the art; preferably a suspension concentrate, a soluble concentrate, an emulsifiable concentrate.

[0022] Specifically, wetting and dispersing agents such as polycarboxylates, lignosulfonates, alkylbenzene or naphthalenesulfonates, fatty alcohol polyoxyethylene ethers, castor oil polyoxyethylene ethers, polynaphthalene formaldehyde sulfonates, phosphate esters, polyethylene glycol series, etc. can be selected; emulsifiers such as Tween series (polyoxyethylene sorbitan fatty acid esters), OP series (alkylphenol polyoxyethylene ethers), alkyl glycosides, etc.; penetrants such as azone, Quick T, JFC, etc.; pH regulators such as monoethanolamine, triethanolamine, etc.; antifreezes such as ethylene glycol, propylene glycol, glycerol, etc.; thickeners such as xanthan gum, magnesium aluminum silicate, sodium carboxymethyl cellulose, etc.; dispersion media such as water, ethanol, dimethyl sulfoxide, xylene, solvent oil, etc.

[0023] To achieve the above object, the technical solution of the application of a pesticide comprising a flumetralin synergistic composition in plant growth regulation in the present invention is as follows:

[0024] The application of a pesticide comprising a flumetralin synergistic composition in plant growth regulation, wherein the plant growth regulation is to inhibit the germination of plant axillary buds, apical growth, the germination and / or growth of new shoots.

[0025] The beneficial effects of the above technical solution are as follows: Through field efficacy experiments, the present invention proves that compared with the application of flumetralin suspension concentrate and flumetralin emulsifiable concentrate, applying the pesticide comprising the flumetralin synergistic composition to plants can significantly inhibit the growth of plant axillary buds, apical growth, and the growth of new shoots, and the effect of inhibiting growth has a long duration and the dosage of flumetralin is small, having great economic and social benefits.

[0026] As a further improvement, the plants are cotton, tobacco, and citrus.

[0027] Specifically, when the pesticide is used in cotton, tobacco, and citrus, it is carried out in the following manner:

[0028] Spray once within 5 days before topping in cotton, focusing on spraying the apical bud part; use the liquid medicine to drench or spray once within 24 hours after manual topping in tobacco; evenly spray once on the outer periphery of the tree crown during the sprouting period of summer shoots in citrus. Description of the Drawings

[0029] Figure 1 It is the growth situation of cotton after the treatment of Example 1 in Experimental Example 1 of the present invention (wherein, from left to right are: the day of applying the medicine, the topping situation 30 days after the medicine, and the growth situation of the top bolls before harvesting);

[0030] Figure 2 It is the growth situation of cotton after the 2nd treatment with 25% flumetralin in Experimental Example 1 of the present invention (wherein, from left to right are: the day of applying the medicine, the topping situation 30 days after the medicine, and the growth situation of the top bolls before harvesting);

[0031] Figure 3 It is the growth situation of cotton in the blank control group in Experimental Example 1 of the present invention (wherein, from left to right are: the day of applying the medicine, the topping situation 30 days after the medicine, and the growth situation of the top bolls before harvesting);

[0032] Figure 4 It is the growth situation of tobacco axillary buds 2 weeks after the treatment of some treatment groups in Experimental Example 2 of the present invention (wherein, from left to right are: Example 4 (Treatment A), 25% flumetralin emulsifiable concentrate (Treatment G), blank control (Treatment CK));

[0033] Figure 5Growth of tobacco axillary buds in some treatment groups after 4 weeks of treatment in Experimental Example 2 of the present invention (from left to right: Example 4 (Treatment A), 25% flumetralin emulsifiable concentrate (Treatment G), blank control (Treatment CK));

[0034] Figure 6 Field performance of living buds of tobacco axillary buds in some treatment groups after 6 weeks of treatment in Experimental Example 2 of the present invention (total of 5 plants, from left to right: Example 4 (Treatment A), 25% flumetralin emulsifiable concentrate (Treatment G), blank control (Treatment CK));

[0035] Figure 7 Phytotoxicity performance after treatment of Comparative Example 2 in Experimental Example 2 of the present invention (left figure is Comparative Example 2, right figure is blank control (Treatment CK));

[0036] Figure 8 Growth of new shoots at different stages after treatment with Example 14 in Experimental Example 3 of the present invention (from left to right are the growth of new shoots at 5 days, 10 days, and 20 days after application);

[0037] Figure 9 Growth of new shoots at different stages after 2 treatments with 40% flumetralin in Experimental Example 3 of the present invention (from left to right are the growth of new shoots at 5 days, 10 days, and 20 days after application);

[0038] Figure 10 Growth of new shoots at different stages after treatment with blank control (CK) in Experimental Example 3 of the present invention (from left to right are the growth of new shoots at 5 days, 10 days, and 20 days after application). Detailed implementation mode

[0039] As a contact and locally systemic plant growth regulator, flumetralin has the effect of inhibiting the growth of tender buds and new shoots, and is often used as an inhibitor for inhibiting the growth of tobacco axillary buds, a topping agent for inhibiting the apical growth of cotton, a pesticide for inhibiting the growth of citrus new shoots, etc. However, excessive or unreasonable use may cause phytotoxicity similar to the side effects of herbicides, such as curled and deformed young leaves, tissue chlorosis with patches or even necrosis. Based on this, the present invention provides a flumetralin synergistic composition, which includes flumetralin and an organic acid (caprylic acid, 1-aminocyclopropanecarboxylic acid or itaconic acid). By utilizing the synergistic effect between the two, while ensuring the drug effect, the usage amount of flumetralin is reduced, the inhibition time of flumetralin is increased, and the safety of flumetralin during application is increased.

[0040] The present invention will be further described in conjunction with specific embodiments. It should be noted that, on the premise of non-conflict, the following-described embodiments or technical features can be arbitrarily combined to form new embodiments. The equipment and raw materials used can be purchased from the market or are commonly used in the art. The methods in the following embodiments are conventional methods in the art unless otherwise specified.

[0041] The pesticides in the following examples and comparative examples were prepared in the following manner:

[0042] The processing process of the aqueous suspension concentrate is as follows: All materials are put into a mixing kettle (which can be replaced by a colloid mill, a sand mill, a shearing machine, etc.), stirred and mixed, then passed through a colloid mill, and then enter the sand mill for three-stage sanding, and finally sheared evenly in a shearing machine. After passing the inspection, it is transferred to a storage tank for filling.

[0043] The processing process of the emulsifiable concentrate and the soluble concentrate is as follows: All liquid materials are put into a reaction kettle or a preparation kettle (which can be replaced by a reaction kettle, etc.), stirred and dissolved completely. After passing the inspection, it can be transferred to a storage tank for filling.

[0044] I. Specific embodiments of a flumetralin synergistic composition of the present invention:

[0045] Example 1

[0046] A flumetralin synergistic composition in this example consists of flumetralin and 1-aminocyclopropanecarboxylic acid; the mass ratio of flumetralin to 1-aminocyclopropanecarboxylic acid is 10:1.

[0047] Example 2

[0048] A flumetralin synergistic composition in this example consists of flumetralin and octanoic acid; the mass ratio of flumetralin to octanoic acid is 1:1.

[0049] Example 3

[0050] A flumetralin synergistic composition in this example consists of flumetralin and itaconic acid; the mass ratio of flumetralin to itaconic acid is 2:5.

[0051] Example 4

[0052] A flumetralin synergistic composition in this example consists of flumetralin and 1-aminocyclopropanecarboxylic acid; the mass ratio of flumetralin to 1-aminocyclopropanecarboxylic acid is 1:1.

[0053] Example 5

[0054] A flumetralin synergistic composition in this example consists of flumetralin and octanoic acid; the mass ratio of flumetralin to octanoic acid is 2:5.

[0055] Example 6

[0056] A flumetralin synergistic composition of this example is composed of flumetralin and itaconic acid; the mass ratio of flumetralin to itaconic acid is 1:4.

[0057] Example 7

[0058] A flumetralin synergistic composition of this example is composed of flumetralin and 1-aminocyclopropane carboxylic acid; the mass ratio of flumetralin to 1-aminocyclopropane carboxylic acid is 5:1.

[0059] Example 8

[0060] A flumetralin synergistic composition of this example is composed of flumetralin and octanoic acid; the mass ratio of flumetralin to octanoic acid is 5:4.

[0061] Example 9

[0062] A flumetralin synergistic composition of this example is composed of flumetralin and itaconic acid; the mass ratio of flumetralin to itaconic acid is 5:6.

[0063] Example 10

[0064] A flumetralin synergistic composition of this example is composed of flumetralin and 1-aminocyclopropane carboxylic acid; the mass ratio of flumetralin to 1-aminocyclopropane carboxylic acid is 40:1.

[0065] Example 11

[0066] A flumetralin synergistic composition of this example is composed of flumetralin and octanoic acid; the mass ratio of flumetralin to octanoic acid is 4:1.

[0067] Example 12

[0068] A flumetralin synergistic composition of this example is composed of flumetralin and itaconic acid; the mass ratio of flumetralin to itaconic acid is 8:5.

[0069] Example 13

[0070] A flumetralin synergistic composition of this example is composed of flumetralin and 1-aminocyclopropane carboxylic acid; the mass ratio of flumetralin to 1-aminocyclopropane carboxylic acid is 4:1.

[0071] Example 14

[0072] A flumetralin synergistic composition of this example is composed of flumetralin and octanoic acid; the mass ratio of flumetralin to octanoic acid is 8:5.

[0073] Example 15

[0074] A flumetralin synergistic composition of this example is composed of flumetralin and itaconic acid; the mass ratio of flumetralin to itaconic acid is 1:1.

[0075] II. Specific embodiments of a pesticide of the present invention comprising a flumetralin synergistic composition:

[0076] Example 16

[0077] The pesticide in this example is a soluble concentrate comprising the flumetralin synergistic composition of Example 1, and is specifically composed of the following components by mass fraction:

[0078] Flumetralin 10%, 1-aminocyclopropanecarboxylic acid 1%, alkyl polyglycoside (0814) 4%, glycerol 20%, dimethyl sulfoxide to make up 100%.

[0079] Example 17

[0080] The pesticide in this example is an emulsifiable concentrate comprising the flumetralin synergistic composition of Example 2, and is specifically composed of the following components by mass fraction:

[0081] Flumetralin 10%, octanoic acid 10%, ethanolamine 3%, xylene 10%, dimethylacetamide 30%, calcium dodecylbenzenesulfonate 6%, ethanol to make up 100%.

[0082] Example 18

[0083] The pesticide in this example is a suspension concentrate comprising the flumetralin synergistic composition of Example 3, and is specifically composed of the following components by mass fraction:

[0084] Flumetralin 10%, itaconic acid 25%, polycarboxylate 5%, fatty alcohol polyoxyethylene ether 2.5%, xanthan gum 0.8%, ethylene glycol 5%, carbendazim 0.05%, water to make up 100%.

[0085] Example 19

[0086] The pesticide in this example is a suspension concentrate comprising the flumetralin synergistic composition of Example 4, and is specifically composed of the following components by mass fraction:

[0087] Flumetralin 10%, 1-aminocyclopropanecarboxylic acid 10%, polynaphthalene formaldehyde sulfonate 5%, fatty alcohol polyoxyethylene ether 4%, magnesium aluminum silicate 1%, xanthan gum 0.25%, ethylene glycol 4%, carbendazim 0.05%, water to make up 100%.

[0088] Example 20

[0089] The pesticide in this example is a soluble concentrate comprising the flumetralin synergistic composition of Example 5, and is specifically composed of the following components by mass fraction:

[0090] Flumetralin 10%, octanoic acid 25%, triethanolamine 15%, polyethylene glycol (600) 5%, OP-10 4%, dimethyl sulfoxide to make up 100%.

[0091] Example 21

[0092] The pesticide in this example is an emulsifiable concentrate comprising the flumetralin synergistic composition of Example 6, and is specifically composed of the following components by mass fraction:

[0093] Flumetralin 10%, itaconic acid 40%, xylene 10%, calcium dodecylbenzenesulfonate 4%, emulsifier 2201 5%, dimethyl sulfoxide to make up 100%.

[0094] Example 22

[0095] The pesticide in this example is an emulsifiable concentrate comprising the flumetralin synergistic composition of Example 7, and is specifically composed of the following components by mass fraction:

[0096] Flumetralin 25%, 1-aminocyclopropanecarboxylic acid 5%, calcium dodecylbenzenesulfonate 4%, castor oil polyoxyethylene ether 4%, solvent oil to make up 100%.

[0097] Example 23

[0098] The pesticide in this example is a suspending agent comprising the flumetralin synergistic composition of Example 8, and is specifically composed of the following components by mass fraction:

[0099] Flumetralin 25%, octanoic acid 20%, ethanolamine 10%, sodium lignosulfonate 4%, isodecyl alcohol polyoxyethylene ether 5%, sodium carboxymethyl cellulose 1%, xanthan gum 0.5%, ethylene glycol 4%, carbendazim 0.05%, and the balance is water.

[0100] Example 24

[0101] The pesticide in this example is a soluble concentrate comprising the flumetralin synergistic composition of Example 9, and is specifically composed of the following components by mass fraction:

[0102] Flumetralin 25%, itaconic acid 30%, fatty alcohol polyoxyethylene ether 5%, alkyl glycoside 7%, azone 2%, dimethyl sulfoxide to make up 100%.

[0103] Example 25

[0104] The pesticide in this example is an emulsifiable concentrate comprising the flumetralin synergistic composition of Example 10, and is specifically composed of the following components by mass fraction:

[0105] Flumetralin 40%, 1-aminocyclopropanecarboxylic acid 1%, calcium dodecylbenzenesulfonate 5%, fatty alcohol polyoxyethylene ether 6%, dimethyl sulfoxide 20%, xylene to make up 100%.

[0106] Example 26

[0107] The pesticide in this example is a soluble concentrate comprising the flumetralin synergistic composition of Example 11, and is specifically composed of the following components by mass fraction:

[0108] Flumetralin 40%, octanoic acid 10%, ethanolamine 3%, polyethylene glycol 10%, Tween 80 6%, Quick T 1.5%, dimethylacetamide to make up 100%.

[0109] Example 27

[0110] The pesticide in this example is a suspending agent comprising the flumetralin synergistic composition of Example 12, and is specifically composed of the following components by mass fraction:

[0111] Flumetralin 40%, itaconic acid 25%, alkylnaphthalenesulfonate 4%, isodecyl alcohol polyoxyethylene ether 4%, magnesium aluminum silicate 0.8%, xanthan gum 0.4%, ethylene glycol 4%, Kathon 0.05%, the balance being water.

[0112] Example 28

[0113] The pesticide in this example is a soluble concentrate comprising the flumetralin synergistic composition of Example 13, and is specifically composed of the following components by mass fraction:

[0114] Flumetralin 40%, 1-aminocyclopropanecarboxylic acid 10%, polyethylene glycol (6000) 8%, OP-10 5%, penetrant JFC 5%, dimethyl sulfoxide to make up 100%.

[0115] Example 29

[0116] The pesticide in this example is an emulsifiable concentrate comprising the flumetralin synergistic composition of Example 14, and is specifically composed of the following components by mass fraction:

[0117] Flumetralin 40%, octanoic acid 25%, ethanolamine 15%, calcium dodecylbenzenesulfonate 5%, alkylphenol polyoxyethylene ether 5%, xylene to make up 100%.

[0118] Example 30

[0119] The pesticide in this example is a suspending agent comprising the flumetralin synergistic composition of Example 15, and is specifically composed of the following components by mass fraction:

[0120] Flumetralin 40%, itaconic acid 40%, alkylnaphthalenesulfonate 6%, isodecyl alcohol polyoxyethylene ether 4%, penetrant Quick T 1.5%, xanthan 0.7%, magnesium aluminum silicate 1%, ethylene glycol 2%, water to make up 100%.

[0121] III. Comparative Examples

[0122] Comparative Example 1

[0123] The difference between the pesticide of this comparative example and that of Example 28 is that it does not include flumetralin, and it is specifically composed of the following components by mass fraction:

[0124] 1-aminocyclopropanecarboxylic acid 10%, polyethylene glycol (6000) 8%, OP-10 5%, penetrant JFC 5%, dimethyl sulfoxide to make up 100%.

[0125] Comparative Example 2

[0126] The difference between the pesticide of this comparative example and that of Example 29 is that it does not include flumetralin, and it is specifically composed of the following components by mass fraction:

[0127] Octanoic acid 25%, ethanolamine 15%, calcium dodecylbenzenesulfonate 5%, alkylphenol polyoxyethylene ether 5%, xylene to make up 100%.

[0128] Comparative Example 3

[0129] The difference between the pesticide of this comparative example and that of Example 30 is that it does not include flumetralin, and it is specifically composed of the following components by mass fraction:

[0130] Itaconic acid 40%, alkylnaphthalenesulfonate 6%, isodecyl alcohol polyoxyethylene ether 4%, penetrant Quick T 1.5%, xanthan gum 0.7%, magnesium aluminum silicate 1%, ethylene glycol 2%, water to make up 100%.

[0131] IV. Experimental Examples Application of the pesticide including the flumetralin synergistic composition in plant growth regulation

[0132] The pesticides prepared in Examples 16 to 30 and Comparative Examples 1 to 3 of the present invention were used on cotton, tobacco and citrus to evaluate their application effects in controlling cotton shaping without topping, tobacco axillary bud inhibition, and citrus new shoot growth inhibition.

[0133] Experimental Example 1 Cotton Topping-Free Experiment

[0134] In this experimental example, the pesticides prepared in Examples 16, 23, 27 and Comparative Examples 1 to 3 were applied to cotton to evaluate their effects on inhibiting the apical growth of cotton. The specific operations are as follows:

[0135] Experiment time: July - September 2024

[0136] Experiment location: Shihezi, Xinjiang

[0137] Experimental agents: Pesticides prepared in Examples 16, 23, 27; Pesticides prepared in Comparative Examples 1 to 3; Commercially available 25% flumetralin suspension; At the same time, manual topping was set as the control group and non-topping was set as the blank control.

[0138] Experimental method: Select a uniform plot in the field. Three days before topping the cotton, each treatment was uniformly sprayed once after diluting with 30 kg of water per mu. The top bud was mainly sprayed (for the commercially available 25% flumetralin suspension, an additional spraying treatment was carried out 20 days after application). The dosages of each treatment group are shown in Table 1.

[0139] Investigation method: Randomly select 30 plants for each treatment. Measure the plant height on the day of application, 30 days and 60 days after application, calculate the increase in plant height, and investigate the number of cotton bolls before the cotton is mature and harvested. Count the number of bolls per plant and the average single boll weight. The specific results are shown in Table 1 and Figures 1 to 3 as follows.

[0140] Table 1 Comparison of plant height increment and yield components of cotton in each treatment group

[0141]

[0142] As can be seen from Table 1 and Figures 1 to 3 it can be known that flumetralin as a cotton topping agent can inhibit the growth of the main stem of cotton, reduce the increase in plant height, coordinate the distribution of nutrients, and promote yield increase. However, the effect of applying the drug twice is only close to that of manual topping, with a large dosage of the drug and high labor costs. And the topping effects of the organic acid single agents in Comparative Examples 1-3 are poor. While for the composition of the present invention, applying the drug only once can approach or achieve the effect of manual topping, and is even better than manual topping in terms of the number of bolls per plant and single boll weight, which are the influencing factors of yield composition, and is beneficial to cotton yield increase. Therefore, using the composition of the present invention can effectively reduce the dosage of flumetralin, extend the lasting period of topping, reduce the number of spraying times, is beneficial to reducing the input cost of the drug, and has the beneficial effect of reducing costs and increasing efficiency.

[0143] Experimental Example 2 Tobacco axillary bud inhibition experiment

[0144] In this experimental example, the pesticides prepared in Examples 19, 20, 21, 25, 26, 27 and Comparative Examples 1-3 were applied to tobacco to evaluate their effects on inhibiting the growth of tobacco axillary buds. The specific operations are as follows:

[0145] Experimental time: April - May 2024

[0146] Experimental location: Tengchong, Yunnan

[0147] Experimental pesticides: The pesticides prepared in Examples 19, 20, 21, 25, 26, 27; the pesticides prepared in Comparative Examples 1-3; commercially available 25% flumetralin suspension and 25% flumetralin suspension oil; at the same time, a water treatment was set as a blank control (CK).

[0148] Experimental method: Set 10 plants per plot as one replication, and repeat each treatment 5 times (a total of 50 plants per treatment). When 50% of the central flowers in the tobacco field are in bloom, manually top the plants, and apply the medicine within 24 hours. When applying the medicine, wipe off the axillary buds longer than 2 cm. When applying the medicine, pour or spray the liquid medicine along the main stem of the plant from the top to the bottom on the axillary bud part, and the application rate is 20 mL per plant. The dosage of each treatment group is shown in Table 2 and Figures 4 to 7 as shown.

[0149] Investigation method: Randomly select 3 plants from each plot, and a total of 15 plants are selected for each treatment. Investigate the number of living buds (counting those longer than 2 cm) once every 2 weeks, 4 weeks, and 6 weeks after applying the medicine, and calculate the bud inhibition rate. The specific results are shown in Table 2.

[0150] Table 2 Comparison of bud inhibition rate and phytotoxicity of each treatment group at different times

[0151]

[0152] Note: ① Bud inhibition rate (%) = (number of living buds in the clear water control group - number of living buds in the treatment group) / number of living buds in the clear water control group * 100;

[0153] ② Degree of phytotoxicity: - No phytotoxicity; + Slight phytotoxicity, does not affect the normal growth of crops; ++ Obvious phytotoxicity, but will gradually recover, will not cause losses or only cause slight losses; +++ Severe phytotoxicity, serious losses in crop yield and quality.

[0154] As can be seen from Table 2 and Figures 4 to 7 it can be seen that flumetralin can inhibit the germination and growth of tobacco axillary buds. The effect of flumetralin emulsifiable concentrate is better than that of the suspension concentrate formulation, and with the passage of time, the bud inhibition rate gradually decreases. The composition of the present invention also has a relatively obvious inhibitory effect on tobacco axillary buds within the ratio range. The highest bud inhibition rate can reach 99.35% 2 weeks after applying the medicine, 97.39% 4 weeks after applying the medicine, and about 90% 6 weeks after applying the medicine. The effect is significantly better than that of each comparative example and the control agent, and the formulation does not affect the effect of the composition. In addition, high-dose treatments of flumetralin emulsifiable concentrate and octanoic acid and itaconic acid will have different degrees of negative impacts on the quality of tobacco. However, the composition of the present invention completely avoids the generation of phytotoxicity caused by high-dose flumetralin or the formulation on the penetration of drug efficacy, and has the advantages of obvious bud inhibition effect, long duration of drug efficacy, and high safety factor.

[0155] Experimental Example 3 Citrus Shoot Control Experiment

[0156] In this experimental example, the pesticides prepared in Examples 28, 29, 30 and Comparative Examples 1-3 were applied to citrus trees to evaluate their effects on inhibiting the growth of summer shoots of citrus trees. The specific operations are as follows:

[0157] Experimental time: June - September 2024

[0158] Experimental location: Guilin, Guangxi

[0159] Experimental agents: pesticides prepared in Examples 28, 29 and 30; pesticides prepared in Comparative Examples 1 to 3; commercially available 40% flumetralin suspension; and clear water treatment as a blank control (CK).

[0160] Experimental method: Each plot was replicated with 5 plants, and each treatment was replicated 3 times (a total of 15 plants in each treatment). During the summer shoot budding period, the crown periphery was sprayed evenly. Flumetralin treatment was applied twice (15 days apart), and the rest of the treatments were applied once, with an application rate of 3L / plant. The dosage of each treatment group is shown in Table 3.

[0161] Survey method: Three new shoots were selected and marked in four directions of east, west, south and north for each fruit tree. The length of new shoots was measured on the day of pesticide application and 5, 10 and 20 days after pesticide application. The inhibition rate of pesticide on summer shoots at each period was calculated. The final fruit setting rate and the yield of marked branches were measured at the fruit ripening period. The specific results are shown in Table 3 and Figures 8 to 10 shown.

[0162] Table 3 Comparison of summer shoot inhibition rate and fruit development in each treatment group at different stages

[0163]

[0164]

[0165] Note: ①Summer shoot inhibition rate (%) = (summer shoot growth of the water control group - summer shoot growth of the treatment group) / summer shoot growth of the water control group * 100, (where the growth refers to the difference between the day after the drug application and the day of drug application);

[0166] ②Fruit setting rate (%) = number of fruits in each treatment at maturity / number of fruits before medication * 100.

[0167] From Table 3 and Figures 8 to 10 It can be seen that two treatments of flumetralin have a good inhibitory effect on summer shoots, while one treatment greatly reduces the inhibition rate of new shoots. The composition of the present invention has a strong inhibitory effect on summer shoots when used once within the ratio range, especially the inhibition rate of new shoots 5d after the application of flumetralin and 1-aminocyclopropanecarboxylic acid (ACC) can reach 100%, which is significantly better than the single-dose treatment of each comparative example and the control agent flumetralin. By controlling the vegetative growth of new shoots, it is beneficial to transfer nutrients to fruits, thereby increasing the fruit setting rate and yield. Combined data can show that the composition of the present invention has the advantages of strong inhibitory effect and long duration of drug effect, effectively reduces the dosage and number of uses of flumetralin, is beneficial to the increase of fruit tree yield and reduces labor costs.

[0168] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. The scope of patent protection of the present invention is subject to the claims. Any equivalent structural changes made by using the content of the specification of the present invention should, by the same token, be included within the protection scope of the present invention.

Claims

1. A flumetralin synergistic composition, characterized in that: The composition consists of flumetralin and an organic acid; the mass ratio of flumetralin to the organic acid is (10-40):(1-40); the organic acid is one of octanoic acid, 1-aminocyclopropanecarboxylic acid and itaconic acid.

2. The synergistic composition of flumetralin according to claim 1, characterized in that: The mass ratio of flumetralin to octanoic acid is (10-40):(10-25).

3. The synergistic composition of flumetralin according to claim 1, characterized in that: The mass ratio of flubendiamide to 1-aminocyclopropanecarboxylic acid is (10-40): (1-10).

4. The synergistic composition of flumetralin according to claim 1, characterized in that: The mass ratio of flumetralin and itaconic acid is (10-40): (25-40).

5. A pesticide comprising the synergistic composition of flumetralin according to any one of 1 to 4, characterized in that: The mass percentage of the flumetralin synergistic composition in the pesticide is 11-80%.

6. The pesticide according to claim 5, characterized in that: The pesticide is in the form of a suspension, a soluble concentrate or an emulsifiable concentrate.

7. Use of a pesticide comprising a synergistic composition of flumetralin as claimed in claim 5 or 6 in plant growth regulation, characterized in that: The plant growth regulation is to inhibit the germination of axillary buds, apical growth, germination of new shoots and / or growth of new shoots of plants.

8. Use of the pesticide comprising the synergistic composition of flumetralin according to claim 7 in plant growth regulation, characterized in that: The plants are cotton, tobacco and citrus.

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