Skin care liposome inclusion as well as preparation method and application thereof

Abstract

The invention relates to the field of skin care products, and discloses a skin care liposome inclusion and a preparation method and application thereof.The skin care liposome inclusion is prepared from egg yolk lecithin, modified collagen, ceramide, caprylic / capric polyethylene glycol glyceride, hyaluronic acid, glycerin, deionized water, plant extract and absolute ethyl alcohol; the modified collagen is prepared by the following steps: carrying out amination treatment on collagen by using hexamethylenediamine and 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride, and then grafting carboxylated konjac glucomannan and carboxylated cholesterol. Carboxylated konjac glucomannan and carboxylated cholesterol are prepared by carboxylating and modifying konjac glucomannan and cholesterol through succinic anhydride, egg yolk lecithin, modified collagen and ceramide are used as film forming materials, and the prepared liposome inclusion has good transdermal absorption capacity and moisturizing and water locking effects.

Description

Technical Field

[0001] The present invention belongs to the technical field of skin care products, and particularly relates to a skin care micro-liposome inclusion and its preparation method and application. Background Art

[0002] The skin is an important reservoir of the human body. The water stored in it accounts for 18-20% of the total body water. The level of its water content is closely related to the health of the skin. Therefore, "moisturizing" is an eternal theme in skin care products. If the skin lacks water, it will lead to epidermal barrier dysfunction and cause various skin problems (such as dryness, freckles, acne, comedones, sensitivity, enlarged pores, dullness, wrinkles, sagging, etc.), and even lead to the occurrence of various skin diseases.

[0003] In recent years, with the changes in dietary structure, air pollution, and the increase in life and work pressure, skin cells are damaged, resulting in a decline in skin resistance, accelerated skin aging, and a decline in the barrier function of the stratum corneum, leading to skin dryness and peeling, sensitivity and flushing, fever, itching, stinging, red rashes, etc., which have become social problems affecting human health. At present, the moisturizing mechanism of cosmetics with moisturizing effects on the market is single, without considering the multiple factors leading to skin water loss and skin water loss caused by skin damage, and it is impossible to achieve comprehensive protection and good moisturizing effects. In addition, although existing skin care products contain active ingredients, due to their weak transdermal ability, their effects cannot be fully exerted, and they cannot meet the market demand. Summary of the Invention

[0004] To solve the deficiencies mentioned in the above background art, the purpose of the present invention is to provide a skin care micro-liposome inclusion and its preparation method and application. Using egg yolk lecithin, modified collagen, and ceramide as film-forming materials, the prepared micro-liposome inclusion has good transdermal absorption ability and moisturizing and water-locking effects.

[0005] The purpose of the present invention can be achieved by the following technical solutions: A skin care micro-liposome inclusion, comprising the following raw materials in parts by weight: 5-15 parts of egg yolk lecithin, 0.5-2 parts of modified collagen, 0.5-3 parts of ceramide, 1-2.5 parts of caprylic / capric polyethylene glycol glyceride, 0.02-0.05 parts of hyaluronic acid, 2-8 parts of glycerol, 7-15 parts of deionized water, 0.01-0.1 parts of plant extract, and 10-20 parts of absolute ethanol; The modified collagen is prepared by subjecting collagen to amidation treatment with hexamethylenediamine and 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride, and then grafting carboxylated konjac glucomannan and carboxylated cholesterol through a chemical reaction. The carboxylated konjac glucomannan and carboxylated cholesterol are prepared by carboxylating konjac glucomannan and cholesterol with succinic anhydride, respectively.

[0006] Preferably, the ceramide is one or a combination of more than one of ceramide IIIB, ceramide NP, ceramide NH, ceramide NDS, ceramide AP, and ceramide NS.

[0007] Preferably, the plant extract is one or a combination of more than one of chamomile extract, ginsenoside extract, lotus extract, resurrection grass extract, and sturgeon roe extract.

[0008] Preferably, the preparation method of the modified collagen comprises the following steps: (1) Dissolve hexamethylenediamine and collagen in deionized water respectively, and use concentrated hydrochloric acid to adjust the pH value of each to 6.2 - 6.8 to obtain a hexamethylenediamine solution and a collagen solution. Then mix the two, add 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride and stir for reaction for 4 - 6 h. Continue to add 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride and stir for reaction for 5 - 7 h. After the reaction is completed, filter to remove the precipitate in the reaction solution, and dialyze in a dialysis bag to remove the unreacted amination reagent to prepare amino collagen. (2) Mix amino collagen, dimethyl sulfoxide, and deionized water to obtain solution one. Mix carboxylated konjac glucomannan, carboxylated cholesterol, dimethyl sulfoxide, and deionized water to obtain solution two. Then mix solution one and solution two, add 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride and 4-dimethylaminopyridine, and stir for reaction for 10 - 12 h. After the reaction is completed, dialyze the mixture to remove the unreacted substances and salts, and finally obtain the modified collagen by freeze-drying.

[0009] Preferably, the concentration of the hexamethylenediamine solution in step (1) is 0.15 - 0.29 g / mL.

[0010] Preferably, the concentration of the collagen solution in step (1) is 0.01 - 0.1 g / mL.

[0011] Preferably, the preparation method of the carboxylated konjac glucomannan in step (2) comprises the following steps: Disperse konjac glucomannan in deionized water, place it under stirring and mixing at 30 - 50 °C, then add succinic anhydride and absolute ethanol, and use sodium hydroxide solution to adjust the pH value to 8 - 9, stir for reaction for 2 - 5 h, then use hydrochloric acid solution to adjust the pH value to 6.5 - 7 to terminate the reaction. After the reaction is completed, rotary evaporate and concentrate the reaction solution to 1 / 3 - 1 / 2 volume, then add 2 - 3 times the volume of ethanol for precipitation extraction, and finally obtain the carboxylated konjac glucomannan through centrifugation, washing, and drying.

[0012] Preferably, the preparation method of carboxylated cholesterol in the step (2) comprises the following steps: taking cholesterol in a reactor, adding ethanol to dissolve, then adding succinic anhydride, triethylamine and 4-dimethylaminopyridine, placing at 55-70 °C and stirring for reaction for 5-7 h, washing with alkaline sodium bicarbonate solution after the reaction is completed, adjusting the pH to 6.5-7 with dilute hydrochloric acid, then washing with deionized water, and purifying with a column chromatography column to prepare carboxylated cholesterol.

[0013] A preparation method of a skin care micro-liposome inclusion body comprises the following steps: weighing each raw material by weight, taking egg yolk lecithin, modified collagen, ceramide, and polyethylene glycol glycerol caprylate / caprate in absolute ethanol, placing at 65-80 °C and stirring for mixing, then adding hyaluronic acid, glycerol, plant extract and deionized water, stirring and mixing evenly, and then placing in a high-pressure homogenizer for homogenization treatment at 70-85 MPa for 2-5 cycles to prepare a skin care micro-liposome inclusion body.

[0014] An application of the skin care micro-liposome inclusion body, dispersing the skin care micro-liposome inclusion body in a cosmetic matrix such as a skin care lotion, cream, facial cleanser, and lotion to prepare a cosmetic.

[0015] The beneficial effects of the present invention: The present invention uses hexamethylenediamine and 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride to amino-functionalize collagen. At the same time, succinic anhydride is used to carboxyl-modify konjac glucomannan and cholesterol. Then, amidation reaction occurs between carboxylated konjac glucomannan, carboxylated cholesterol and amino-functionalized collagen, so that konjac glucomannan and cholesterol are grafted into the collagen structure through strong chemical bonds. Among them, konjac glucomannan aggregates to form supramolecules through hydrogen bonds, dipoles and other interactions with water molecules, and has good thickening and gelling properties, which significantly improves the stability of the micro-liposome encapsulation, reduces aggregation and precipitation, and can extend the shelf life of the product. In addition, the stable micro-liposome encapsulation is easier to penetrate the skin barrier, ensuring the effective delivery of active ingredients, and at the same time can enhance its compatibility with the skin cutin layer, making the micro-liposome encapsulation easier to spread on the skin surface and promoting the penetration of active ingredients. Collagen can improve the skin color, fine lines and wrinkles. Grafting lipophilic cholesterol onto the hydrophilic collagen molecular chain makes the grafted collagen amphiphilic and can self-assemble into micelles in aqueous solution, enhancing the skin's absorption of collagen. The present invention uses egg yolk lecithin, modified collagen and ceramide as film-forming materials. The prepared micro-liposome encapsulation can efficiently transdermally deliver the active ingredients encapsulated therein, efficiently penetrate into the basal layer of the skin, absorb more water and nutrients, maximize the compensation for the deficiency of dermal nutrition transmission, and has excellent stability and skin tolerance. In addition, because ceramide is added therein, it can also renew the natural protective layer of the skin and form an effective barrier to prevent water loss. Detailed implementation manners

[0016] The following will combine the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0017] Example 1 A preparation method of carboxylated konjac glucomannan comprises the following steps: Take 2.5 g of konjac glucomannan and disperse it in 500 mL of deionized water, place it under stirring and mixing at 45 °C, then add 1 g of succinic anhydride and 50 mL of absolute ethanol, and adjust the pH value to 8 with sodium hydroxide solution, stir and react for 4 h, then adjust the pH value to 7 with hydrochloric acid solution to terminate the reaction. After the reaction is completed, the reaction solution is rotary evaporated and concentrated to 1 / 3 volume, then 3 times the volume of ethanol is added for precipitation extraction, and finally it is centrifuged, washed and dried to prepare carboxylated konjac glucomannan.

[0018] Example 2 A preparation method of carboxylated cholesterol comprises the following steps: Take 1.2 g of cholesterol in a reactor, add 50 mL of ethanol to dissolve it, then add 3.6 g of succinic anhydride, 0.5 mL of triethylamine and 0.2 g of 4-dimethylaminopyridine, place it at 60 °C and stir for reaction for 7 h. After the reaction is completed, wash it with 0.1 mol / L basic sodium bicarbonate solution, adjust the pH to 6.5 with dilute hydrochloric acid, then wash it with deionized water, and purify it with a column chromatography column to prepare carboxylated cholesterol.

[0019] Example 3 A method for preparing modified collagen includes the following steps: (1) Take 1.6 g of hexamethylenediamine and 0.5 g of collagen and dissolve them in 10 mL of deionized water respectively, and adjust the pH value of each to 6.5 with concentrated hydrochloric acid to obtain a hexamethylenediamine solution and a collagen solution. Then mix the two, add 0.1 g of 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride and stir for reaction for 5 h. Continue to add 0.05 g of 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride and stir for reaction for 6 h. After the reaction is completed, filter to remove the precipitate in the reaction solution, and dialyze in a dialysis bag to remove the unreacted amination reagent to prepare aminoated collagen. (2) Take 0.4 g of aminoated collagen, 2 mL of dimethyl sulfoxide and 12 mL of deionized water and mix them to obtain Solution 1. Take 0.2 g of carboxylated konjac glucomannan prepared in Example 1, 0.2 g of carboxylated cholesterol prepared in Example 2, 4 mL of dimethyl sulfoxide and 24 mL of deionized water and mix them to obtain Solution 2. Then mix Solution 1 and Solution 2, add 0.1 g of 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride and 0.01 g of 4-dimethylaminopyridine, stir for reaction for 12 h. After the reaction is completed, dialyze the mixture to remove the unreacted substances and salts, and finally perform freeze-drying to prepare modified collagen.

[0020] Example 4 A skin care micro-liposome inclusion body includes the following raw materials in parts by weight: 8 parts of egg yolk lecithin, 0.6 part of modified collagen prepared in Example 3, 0.7 part of ceramide IIIB, 1.2 parts of caprylic / capric polyethylene glycol glycerol ester, 0.02 part of hyaluronic acid, 3 parts of glycerol, 7 parts of deionized water, 0.02 part of chamomile extract, and 12 parts of absolute ethanol.

[0021] The preparation method of the above skin care micro-liposome inclusion body includes the following steps: Weigh each raw material according to parts by weight. Take egg yolk lecithin, modified collagen, ceramide IIIB, and caprylic / capric polyethylene glycol glycerol ester in absolute ethanol, place it at 80 °C and stir to mix. Then add hyaluronic acid, glycerol, chamomile extract and deionized water, stir and mix evenly, and then place it in a high-pressure homogenizer for homogenization treatment at 80 MPa for 3 cycles to prepare the skin care micro-liposome inclusion body.

[0022] Example 5 A skin care micro-liposome inclusion, comprising the following raw materials in parts by weight: 10 parts of egg yolk lecithin, 1.1 parts of the modified collagen prepared in Example 3, 1.2 parts of ceramide IIIB, 1.8 parts of caprylic / capric polyethylene glycol glyceride, 0.03 parts of hyaluronic acid, 5 parts of glycerol, 11 parts of deionized water, 0.05 parts of chamomile extract, and 14 parts of absolute ethanol.

[0023] The preparation method of the above skin care micro-liposome inclusion is the same as that of Example 4.

[0024] Example 6 A skin care micro-liposome inclusion, comprising the following raw materials in parts by weight: 14 parts of egg yolk lecithin, 1.6 parts of the modified collagen prepared in Example 3, 2.7 parts of ceramide IIIB, 2.2 parts of caprylic / capric polyethylene glycol glyceride, 0.05 parts of hyaluronic acid, 7 parts of glycerol, 15 parts of deionized water, 0.06 parts of chamomile extract, and 18 parts of absolute ethanol.

[0025] The preparation method of the above skin care micro-liposome inclusion is the same as that of Example 4.

[0026] Comparative Example 1 A preparation method of modified collagen comprises the following steps: (1) Take 1.6 g of hexamethylenediamine and 0.5 g of collagen and dissolve them in 10 mL of deionized water respectively, and adjust the pH value of each to 6.5 with concentrated hydrochloric acid to obtain a hexamethylenediamine solution and a collagen solution. Then mix the two, add 0.1 g of 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride and stir for reaction for 5 h. Continue to add 0.05 g of 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride and stir for reaction for 6 h. After the reaction is completed, filter to remove the precipitate in the reaction solution, and dialyze in a dialysis bag to remove the unreacted amination reagent to prepare aminoated collagen; (2) Take 0.4 g of aminoated collagen, 2 mL of dimethyl sulfoxide and 12 mL of deionized water and mix them to obtain Solution 1. Take 0.2 g of the carboxylated cholesterol prepared in Example 2, 2 mL of dimethyl sulfoxide and 12 mL of deionized water and mix them to obtain Solution 2. Then mix Solution 1 and Solution 2, add 0.1 g of 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride and 0.01 g of 4-dimethylaminopyridine, and stir for reaction for 12 h. After the reaction is completed, dialyze the mixture to remove the unreacted substances and salts, and finally perform freeze-drying to prepare the modified collagen.

[0027] Comparative Example 2 A skin care micro-liposome inclusion, comprising the following raw materials in parts by weight: 14 parts of egg yolk lecithin, 1.6 parts of the modified collagen prepared in Comparative Example 1, 2.7 parts of ceramide IIIB, 2.2 parts of caprylic / capric polyethylene glycol glyceride, 0.05 parts of hyaluronic acid, 7 parts of glycerol, 15 parts of deionized water, 0.06 parts of chamomile extract, and 18 parts of absolute ethanol.

[0028] The preparation method of the above-mentioned skin care micro-liposome inclusion is the same as that of Example 4.

[0029] Comparative Example 3 A skin care micro-liposome inclusion, comprising the following raw materials in parts by weight: 14 parts of egg yolk lecithin, 0.4 parts of cholesterol, 0.8 parts of collagen, 0.4 parts of konjac glucomannan, 2.7 parts of ceramide IIIB, 2.2 parts of caprylic / capric polyethylene glycol glyceride, 0.05 parts of hyaluronic acid, 7 parts of glycerol, 15 parts of deionized water, 0.06 parts of chamomile extract, and 18 parts of absolute ethanol.

[0030] The preparation method of the above-mentioned skin care micro-liposome inclusion comprises the following steps: Weigh each raw material according to the parts by weight, take egg yolk lecithin, cholesterol, ceramide IIIB, and caprylic / capric polyethylene glycol glyceride and place them in absolute ethanol, stir and mix at 80 °C, then add collagen, hyaluronic acid, glycerol, konjac glucomannan, chamomile extract, and deionized water, stir and mix evenly, and then place them in a high-pressure homogenizer for homogenization treatment at 80 MPa for 3 cycles to prepare the skin care micro-liposome inclusion.

[0031] Performance testing A. Perform stability testing on the micro-liposome inclusions prepared in Examples 4-6: Use dynamic light scattering technology to measure the particle size, PDI (polymer dispersity index), and Zeta potential of the samples. Place the micro-liposome inclusion solution in a storage condition of normal temperature and dark, and take samples after 7, 21, and 60 days. Use a Malvern particle size analyzer to measure the particle size, PDI, and Zeta potential, and the data results are shown in Table 1.

[0032] Table 1 Results of sample stability testing

[0033] It can be seen from the data in Table 1 that after 60 days of storage in the dark, the particle size of the micro-liposome inclusions prepared in Examples 4-6 of the present invention slightly increases, but the change is not obvious, and the absolute value of the potential is above 30 mV, showing good stability.

[0034] B. Prepare a moisturizing and repairing cream with the micro-liposome inclusions prepared in Examples 4-6 and Comparative Examples 2-3: Heat the raw materials in Phase C to 60 °C, stir until completely dissolved and uniform, and set aside after cooling; put the raw materials in Phase A into an emulsifying pot and stir at 30 rpm for 30 minutes to mix evenly; put the raw materials in Phase B and the pre-dissolved and cooled raw materials in Phase C into a water phase pot and stir at 60 rpm for 20 minutes to mix evenly; under stirring, slowly put the raw materials in the water phase pot into the emulsifying pot, and continue to stir at 100 rpm for 40 minutes to mix evenly, and cool to room temperature to obtain the moisturizing and repairing cream, where the raw materials in Phases A, B, and C are shown in Table 2.

[0035] Table 2 Moisturizing and Repairing Cream Formula

[0036] (1) Skin irritation test: Thirty-two subjects were selected. 0.025 g of the prepared cream containing micro-liposome inclusions was applied to the chambers of the patch test device, and a special tape was applied externally to the back or the flexor side of the forearm of the subjects. After 24 h, the patch test device was removed. If there was any remaining product, it was gently wiped off with a moist tissue. The skin reactions were observed at 0.5, 24, and 48 h, respectively, and the results were recorded according to the skin reaction grading standard in Table 3: Table 3 Skin Reaction Grading Standard

[0037] Through the occlusive patch test, none of the 32 subjects showed any adverse reactions after using the cream containing micro-liposome inclusions for 0.5, 24, and 48 h. There were no symptoms such as erythema, burning sensation, itching, etc., showing a negative reaction, indicating that the prepared micro-liposome inclusions and cream were non-irritating to the skin.

[0038] (2) In vitro transdermal performance test: The vertical Franz diffusion cell method was used for the transdermal experiment on excised rat skin. The abdominal skin of SD female rats was fixed between the receiving chamber and the supply chamber. 1 g of the cream containing micro-liposome inclusions prepared in Examples 4-6 and Comparative Examples 2-3 was placed in the supply chamber. A physiological saline solution containing 2% sodium dodecyl sulfate and 20% ethanol by mass fraction was used as the receiving solution. Stirring diffusion was carried out at 37 °C at 300 r / min. 0.5 mL of the receiving solution was taken at 8 h, and an equal amount of fresh receiving solution at a constant temperature was immediately replenished. HPLC was used to analyze the cumulative permeation amount of the micro-liposome inclusions, and the data results are shown in Table 4.

[0039] (3) Moisturizing performance test: Forty subjects with an average age of 25-45 years, including 20 females and 20 males, were randomly divided into 5 groups of 8 each. The skin of the subjects was healthy and met the inclusion criteria. At room temperature of 20 °C and relative humidity of 35%, it was stabilized for at least half an hour. A 5 cm × 5 cm test area was drawn on the inner side of the right forearm of the subjects. The skin in the test area was torn 10 times with tape and then the cream containing micro-liposome inclusions prepared in Examples 4-6 and Comparative Examples 2-3 was applied. The sample usage amount was 1 g for each. Then, a skin moisture content tester was used to measure the moisture content of the skin before being damaged, after being damaged, and 2 h after applying the sample, respectively, and repeated 3 times to obtain the average value, and the skin moisture content growth rate was calculated. During the experiment, the subjects were not allowed to apply any other cosmetics to the experimental site. The formula for calculating the skin moisture content growth rate is: Skin moisture content growth rate = (M 1 -M 0 ) / M 0 × 100%, where M0 is the skin water content before use; M 1 is the skin water content after 2 hours of use, and the data results are shown in Table 4 below.

[0040] Table 4 Test results of in vitro transdermal performance and moisturizing performance of the samples

[0041] As can be seen from the data in Table 4, the micro-liposome encapsulates prepared in Examples 4-5 of the present invention have good transdermal absorption ability and moisturizing and water-locking effects. Among them, the modified collagen added in Comparative Example 2 does not contain carboxylated konjac glucomannan, and its measured cumulative permeation amount and the growth rate of skin moisture content are lower than those of Examples 4-6. The reason is that the grafting of carboxylated konjac glucomannan can make the micro-liposome encapsulate more easily penetrate the skin barrier and at the same time improve the moisturizing effect. In Comparative Example 3, cholesterol, collagen, and konjac glucomannan were simply mixed, and its measured cumulative permeation amount and the growth rate of skin moisture content were lower than those of Examples 4-6, indicating that the modification of collagen can promote the penetration of active ingredients and enhance the moisturizing effect.

[0042] In the description of this specification, the descriptions referring to the terms "one embodiment", "example", "specific example", etc. mean that the specific features, structures, materials or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in a suitable manner in any one or more embodiments or examples.

[0043] The above shows and describes the basic principles, main features and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited by the above embodiments, and the above embodiments and the descriptions in the specification only illustrate the principles of the present invention. Without departing from the spirit and scope of the present invention, the present invention will have various changes and improvements, and these changes and improvements all fall within the scope of the present invention claimed.

Claims

1. A skin care liposome inclusion, characterized in that: The invention comprises the following raw materials in parts by weight: 5-15 parts of egg yolk lecithin, 0.5-2 parts of modified collagen, 0.5-3 parts of ceramide, 1-2.5 parts of caprylic acid capric acid macrogol glyceride, 0.02-0.05 parts of hyaluronic acid, 2-8 parts of glycerol, 7-15 parts of deionized water, 0.01-0.1 parts of plant extract, and 10-20 parts of anhydrous ethanol; The modified collagen is prepared by subjecting collagen to amino treatment using hexamethylenediamine and 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride, and then grafting carboxylated konjac glucomannan and carboxylated cholesterol using chemical reaction. The carboxylated konjac glucomannan and carboxylated cholesterol are prepared by subjecting konjac glucomannan and cholesterol to carboxylation modification using succinic anhydride, respectively.

2. The skin care liposome inclusion according to claim 1, characterized in that: The ceramide is one or more combinations of ceramide IIIB, ceramide NP, ceramide NH, ceramide NDS, ceramide AP, and ceramide NS.

3. The skin care liposome inclusion according to claim 1, characterized in that: The plant extract is one or more combinations of chamomile extract, ginsenoside extract, lotus extract, resurrection grass extract and sturgeon caviar extract.

4. The skin care liposome inclusion according to claim 1, characterized in that: The preparation method of the modified collagen comprises the following steps: (1) Dissolve hexamethylenediamine and collagen in deionized water respectively, and adjust the pH value of each to 6.2-6.8 with concentrated hydrochloric acid to obtain a hexamethylenediamine solution and a collagen solution, then mix the two, add 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride, stir and react for 4-6 hours, continue to add 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride, stir and react for 5-7 hours, filter and remove the precipitate in the reaction solution after the reaction is completed, dialyze in a dialysis bag to remove the unreacted amination reagent, and prepare the amino collagen; (2) Aminated collagen, dimethyl sulfoxide and deionized water are mixed to obtain solution 1, carboxylated konjac glucomannan, carboxylated cholesterol, dimethyl sulfoxide and deionized water are mixed to obtain solution 2, and then solution 1 and solution 2 are mixed, 1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride and 4-dimethylaminopyridine are added, and the mixture is stirred for 10 to 12 hours. After the reaction is completed, the mixture is dialyzed to remove unreacted products and salts, and finally freeze-dried to prepare modified collagen.

5. The skin care liposome inclusion according to claim 4, characterized in that: The concentration of the hexamethylenediamine solution in step (1) is 0.15-0.29 g / mL.

6. The skin care liposome inclusion according to claim 4, characterized in that: The concentration of the collagen solution in step (1) is 0.01-0.1 g / mL.

7. The skin care liposome inclusion according to claim 4, characterized in that: The preparation method of carboxylated konjac glucomannan in step (2) comprises the following steps: dispersing konjac glucomannan in deionized water, stirring and mixing at 30-50° C., then adding succinic anhydride and anhydrous ethanol, and adjusting the pH value to 8-9 with sodium hydroxide solution, stirring and reacting for 2-5 hours, and then adjusting the pH value to 6.5-7 with hydrochloric acid solution to terminate the reaction, and after the reaction is completed, concentrating the reaction solution by rotary evaporation to 1 / 3-1 / 2 volume, and then adding 2-3 times volume of ethanol for precipitation extraction, and finally centrifuging, washing, and drying to prepare carboxylated konjac glucomannan.

8. The skin care liposome inclusion according to claim 4, characterized in that: The method for preparing carboxylated cholesterol in step (2) comprises the following steps: taking cholesterol into a reactor, adding ethanol to dissolve it, then adding succinic anhydride, triethylamine and 4-dimethylaminopyridine, stirring and reacting at 55-70°C for 5-7 hours, washing with alkaline sodium bicarbonate solution after the reaction is completed, adjusting the pH to 6.5-7 with dilute hydrochloric acid, then washing with deionized water, and purifying with column chromatography to prepare carboxylated cholesterol.

9. A method for preparing the skin care liposome inclusion according to any one of claims 1 to 8, characterized in that: The method comprises the following steps: weighing each raw material by weight, taking egg yolk lecithin, modified collagen, ceramide, and caprylic / capric acid macrogol glyceride in anhydrous ethanol, stirring and mixing at 65-80°C, then adding hyaluronic acid, glycerin, plant extract and deionized water, stirring and mixing, and placing in a high-pressure homogenizer for 70-85MPa, 2-5 cycles of homogenization treatment, so as to prepare skin care liposome inclusions.

10. An application of the skin care liposome inclusion according to claim 1, characterized in that: The skin care liposome inclusions are dispersed in a cosmetic matrix of skin care lotion, cream, facial cleanser, and lotion to prepare cosmetics.

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