A biomimetic selenium-doped fiber membrane and its preparation method and application
Selenium-containing fibers are prepared through electrospinning technology and combined with macrophage membranes to produce bionic selenium-doped fiber membranes, which solves the immunomodulation and bone regeneration problems of electrospinning fiber materials in an inflammatory environment, and achieves the multifunctional synergy between inflammatory factor removal and bone regeneration.
Patent Information
- Application Number
- CN202510526442.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2025-04-25
- Publication Date
- 2025-07-22
- Estimated Expiration
- 2045-04-25
AI Technical Summary
The existing electrospinned fiber materials have insufficient immune regulation capabilities in inflammatory environments, cannot effectively remove inflammatory factors, and the bone regeneration effect is not ideal.
Electrospinning technology is used to prepare selenium-containing electrospinning fibers and mix them with macrophage membranes. A bionic selenium-doped fiber membrane is prepared through ultrasonic shock to achieve efficient coating of macrophage membranes on the fiber surface, and has good biological activity and selenium release ability.
Bionic selenium-doped fiber membranes can effectively remove inflammatory factors, regulate macrophage polarization, have bone immune regulation capabilities, promote bone regeneration, and are suitable for the treatment of infectious bone defects.
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Figure CN120037217B_ABST
Abstract
Description
Technical Field
[0001] The invention relates to the technical field of medical materials, and in particular to a bionic selenium-doped fiber membrane and a preparation method and application thereof. Background Art
[0002] Infected bone defects are a common orthopedic disease, usually caused by trauma, infection or tumor resection. Traditional bone repair materials such as autologous bone transplantation, allogeneic bone transplantation and metal implants have problems such as limited donors, immune rejection and high risk of infection. In recent years, electrospun fibers, as a material with good biocompatibility and degradability, have been widely used in tissue engineering and drug delivery. However, the immunomodulatory ability and bone regeneration effect of existing electrospun fiber materials in inflammatory environments still need to be improved.
[0003] In the prior art, electrospun fiber materials mainly promote tissue repair by loading drugs or growth factors, but in the inflammatory environment of infected bone defects, excessive inflammatory response will inhibit the bone regeneration process. In addition, the existing electrospun fiber materials lack the ability to clear inflammatory factors and cannot effectively regulate the bone immune microenvironment. Therefore, it is of great significance to develop an electrospun fiber material that can regulate the bone immune microenvironment and promote bone regeneration under inflammatory conditions. Summary of the invention
[0004] In order to solve the above technical problems, the purpose of the present invention is to provide a bionic selenium-doped fiber membrane and its preparation method and application, so as to solve the problems that the existing electrospun fiber materials have insufficient immunoregulatory ability in inflammatory environments and cannot effectively eliminate inflammatory factors; the ability to regulate macrophage polarization is limited and cannot effectively inhibit excessive inflammatory responses; the bone regeneration effect in inflammatory environments is not ideal, and the like.
[0005] The technical solution of the present invention to solve the above technical problems is as follows:
[0006] The first aspect of the present invention provides a method for preparing a bionic selenium-doped fiber membrane, comprising the following steps:
[0007] (1) firstly, polylactic acid-glycolic acid copolymer and sodium selenite are mixed and stirred in a solvent to prepare a spinning solution, then electrospinning is performed, and finally drying is performed to obtain selenium-containing electrospun fibers;
[0008] (2) The selenium-containing electrospun fibers obtained in step (1) are mixed with macrophage membranes and subjected to ultrasonic vibration to obtain a product.
[0009] The beneficial effects of the present invention are as follows: The present invention uses electrospinning technology to prepare selenium-containing fiber membranes, which has a simple process, low cost, and is easy to scale up production. By adjusting the electrospinning parameters (such as voltage, spinning speed, solvent ratio, etc.), the pore size, thickness, and mechanical properties of the fiber membranes can be precisely controlled to meet the requirements of different application scenarios.
[0010] Further, in step (1), the concentration of poly(lactic-co-glycolic acid) copolymer in the spinning solution is 10 w / v % - 30 w / v %; the mass ratio of poly(lactic-co-glycolic acid) copolymer to sodium selenite is (90 - 95):(5 - 10).
[0011] Preferably, in step (1), the concentration of poly(lactic-co-glycolic acid) copolymer in the spinning solution is 20 w / v %; the mass ratio of poly(lactic-co-glycolic acid) copolymer to sodium selenite is 92.25:7.75.
[0012] Further, in step (1), the solvent is a mixed solvent composed of dichloromethane and dimethylformamide; the volume ratio of dichloromethane to dimethylformamide is (1 - 5):1.
[0013] Further, the conditions for electrospinning in step (1) are as follows: the distance between the nozzle and the receiving roller is 15 - 20 cm, the spinning speed is 1 - 5 mL / h, the spinning voltage is 20 - 25 kV, and the spinning environment temperature is 20 - 30 °C; the drying temperature is 50 - 70 °C, and the time is 20 - 30 h.
[0014] The beneficial effects of adopting the above further technical solution are as follows: The present invention prepares selenium-containing electrospun fibers through a chemical synthesis method, ensuring that they can have good biological activity and the ability to release selenium elements.
[0015] Further, in step (2), the macrophage cell membrane is prepared by the following method: First, the macrophages are suspended in phosphate buffer solution, then subjected to freeze-thaw cycle treatment, and finally centrifuged and separated to obtain it.
[0016] Further, the conditions for the freeze-thaw cycle treatment are as follows: First, freeze at -90~-70 °C, then thaw at 35 - 40 °C, and repeat the freezing and thawing 2 - 5 times; the centrifugation speed is 8000 - 12000 rpm, and the time is 5 - 15 min.
[0017] The beneficial effects of adopting the above further technical solution are as follows: The present invention prepares macrophage cell membranes by the repeated freeze-thaw method, ensuring the purity and biocompatibility of the prepared cell membranes, providing excellent cell compatibility for the subsequent prepared biomimetic selenium-doped fiber membranes, and enabling them to support the normal growth of cells.
[0018] Further, the mass ratio of the selenium-containing electrospun fibers to the macrophage cell membrane in step (2) is (5-20):1.
[0019] Further, the frequency of ultrasonic oscillation in step (2) is 30-50 kHz, and the time is 10-60 min.
[0020] The beneficial effects of adopting the above further technical solutions are as follows: By using ultrasonic oscillation, through physical adsorption and ultrasonic-assisted self-assembly technology, the present invention realizes the efficient coating of the macrophage cell membrane on the fiber surface.
[0021] In the second aspect of the present invention, a bionic selenium-doped fiber membrane is provided, which is prepared by the above preparation method.
[0022] The beneficial effects of the present invention are as follows: The bionic selenium-doped fiber membrane prepared by the present invention has a multi-functional integrated design, integrating immunomodulation, inflammatory factor clearance, macrophage polarization regulation, and tissue repair promotion functions, realizing multi-functional synergistic effects, enabling it not only to regulate the immune microenvironment but also to have potential bone regeneration ability. At the same time, the porous structure and bionic characteristics of the fiber membrane are beneficial to cell adhesion, proliferation, and differentiation.
[0023] In the third aspect of the present invention, the application of the above bionic selenium-doped fiber membrane in the preparation of medical devices or drugs for treating infectious bone defects is provided.
[0024] The beneficial effects of the present invention are as follows: The bionic selenium-doped fiber membrane prepared by the present invention can effectively clear inflammatory factors, regulate macrophage polarization, has bone immune regulation ability, and has broad application prospects and practical value in the treatment of infectious bone defects. After the fiber membrane is implanted, the macrophage cell membrane on its surface can adsorb and clear local inflammatory factors (such as TNF-α, IL-1β, etc.) through membrane surface receptors (such as tumor necrosis factor receptors, interleukin receptors, etc.), thereby restoring the bone immune microenvironment. At the same time, the slowly released selenium element in the fiber membrane regulates the polarization of macrophages from the M1 type to the M2 type, inhibiting the inflammatory response.
[0025] The present invention has the following beneficial effects:
[0026] The present invention uses electrospinning technology to prepare a bionic selenium-doped fiber membrane. The preparation process is simple, the cost is low, it is easy to scale up production. The prepared bionic selenium-doped fiber membrane has good biological activity and selenium element release ability, has excellent immunomodulation ability, can effectively clear inflammatory factors in vitro, regulate macrophage polarization, has bone immune regulation ability, can effectively treat infectious bone defects, and has broad application prospects and practical value. Brief Description of the Drawings
[0027] Figure 1Transmission electron microscope image of the fiber filaments of the biomimetic selenium-doped fiber membrane prepared in Example 1;
[0028] Figure 2 Scanning electron microscope image of the PLGA fiber membrane prepared in Comparative Example 1;
[0029] Figure 3 Scanning electron microscope image of the selenium-containing fiber membrane prepared in Comparative Example 2;
[0030] Figure 4 ELISA test result graph for the experiment of clearing inflammatory factors in Test Example 1, where a is the IL-1β inflammatory factor and b is the TNF-α inflammatory factor;
[0031] Figure 5 qPCR test result graph for the macrophage polarization regulation experiment in Test Example 2, where a is the macrophage inflammatory differentiation marker IL-1β, b is the macrophage inflammatory differentiation marker IL-6, c is the macrophage pro-repair differentiation marker Arg-1, and d is the macrophage pro-repair differentiation marker CD206. Detailed implementation mode
[0032] The principles and features of the present invention are described below in conjunction with the accompanying drawings. The examples given are only for explaining the present invention and are not intended to limit the scope of the present invention. For those not specified in the examples, conventional conditions or conditions recommended by the manufacturer are used. For reagents or instruments not specified by the manufacturer, they are all conventional products that can be obtained through commercial purchase.
[0033] Example 1:
[0034] A preparation method of a biomimetic selenium-doped fiber membrane, comprising the following steps:
[0035] (1) Preparation of selenium-containing spinning solution:
[0036] First, use a precision analytical balance to weigh PLGA (polylactic acid-glycolic acid copolymer), and use dichloromethane (DCM) and dimethylformamide (DMF) in a volume ratio of 2.5:1 as the solvent. Dissolve PLGA in the mixed solvent and place it on a magnetic stirrer for continuous stirring for 12 hours to prepare a 20 w / v% PLGA spinning solution; then take a certain mass of sodium selenite (Na2SeO3) powder (the mass ratio of sodium selenite to PLGA is 7.75:92.25) and add it to the PLGA spinning solution, and continuously stir on a magnetic stirrer for 3 h to obtain a selenium-containing spinning solution.
[0037] (2) Electrospinning
[0038] Connect the selenium-containing spinning solution obtained in step (1) to a spinning nozzle, and set the parameters of the electrospinning equipment as follows: the distance between the nozzle and the receiving roller is 18 cm, the spinning speed is 3 mL / h, the voltage is 22 kV, the ambient temperature inside the electrospinning equipment is 25 °C, the roller at the bottom rotates at a low speed and the fibers are collected with aluminum foil covered. After the spinning is completed, dry the residual solvent in a vacuum drying oven, the drying temperature is 60 °C, and the time is 24 h to obtain a selenium-containing electrospun fiber membrane.
[0039] (3) Preparation of macrophage cell membranes
[0040] Extract macrophage cell membranes from macrophages by the repeated freeze-thaw method. The specific steps are as follows: suspend macrophages in PBS buffer, and after three freeze-thaw cycles (-80 °C freezing, 37 °C thawing), then centrifuge at 10000 rpm for 10 min to obtain macrophage cell membranes.
[0041] (4) Preparation of biomimetic selenium-doped fiber membranes
[0042] Mix the macrophage cell membranes obtained in step (3) and the selenium-containing electrospun fibers obtained in step (2) at a mass ratio of 1:10, place them in an ultrasonic oscillator, and oscillate at a frequency of 40 kHz for 30 min to ensure that the macrophage cell membranes uniformly wrap the fiber surface, and obtain a biomimetic selenium-doped fiber membrane (MSe@Fiber).
[0043] The transmission electron micrograph of the fiber filaments of the biomimetic selenium-doped fiber membrane prepared in this example is as Figure 1 shown, and it can be clearly seen that the fiber filaments are wrapped with clear cell membranes on the periphery.
[0044] Example 2:
[0045] A method for preparing a biomimetic selenium-doped fiber membrane, comprising the following steps:
[0046] (1) Preparation of selenium-containing spinning solution:
[0047] First, use an analytical balance to weigh PLGA (poly(lactic-co-glycolic acid)), and use dichloromethane (DCM) and dimethylformamide (DMF) as solvents in a volume ratio of 2.5:1. Dissolve PLGA in the mixed solvent, and place it on a magnetic stirrer and stir continuously for 12 hours to prepare a 15 w / v % PLGA spinning solution; then take a certain mass of sodium selenite (Na2SeO3) powder (the mass ratio of sodium selenite to PLGA is 7.75:92.25) and add it to the PLGA spinning solution, and stir continuously on a magnetic stirrer for 3 h to obtain a selenium-containing spinning solution.
[0048] (2) Electrospinning
[0049] Connect the selenium-containing spinning solution obtained in step (1) to a spinning nozzle. The parameters of the electrospinning equipment are set as follows: the distance between the nozzle and the receiving roller is 20 cm, the spinning speed is 3 mL / h, the voltage is 22 kV, the ambient temperature inside the electrospinning equipment is 25 °C, the roller at the bottom rotates at a low speed and the fibers are collected with aluminum foil covered. After spinning, it is dried in a vacuum drying oven to volatilize the residual solvent. The drying temperature is 60 °C and the time is 24 h to obtain a selenium-containing electrospun fiber membrane.
[0050] (3)Preparation of macrophage cell membrane
[0051] Extract the macrophage cell membrane from macrophages by the repeated freeze-thaw method. The specific steps are as follows: Suspend the macrophages in PBS buffer, perform three freeze-thaw cycles (-80 °C freezing, 37 °C thawing), and then centrifuge at 10,000 rpm for 10 min to obtain the macrophage cell membrane.
[0052] (4)Preparation of biomimetic selenium-doped fiber membrane
[0053] Mix the macrophage cell membrane obtained in step (3) and the selenium-containing electrospun fiber obtained in step (2) at a mass ratio of 1:20, place it in an ultrasonic oscillator, and oscillate at a frequency of 50 kHz for 30 min to ensure that the macrophage cell membrane uniformly wraps the fiber surface to obtain a biomimetic selenium-doped fiber membrane.
[0054] Example 3:
[0055] A method for preparing a biomimetic selenium-doped fiber membrane, comprising the following steps:
[0056] (1)Preparation of selenium-containing spinning solution:
[0057] First, weigh PLGA (poly(lactic-co-glycolic acid)) using a precision analytical balance. Using dichloromethane (DCM) and dimethylformamide (DMF) as solvents in a volume ratio of 2.5:1, dissolve PLGA in the mixed solvent, and place it on a magnetic stirrer and continuously stir for 12 hours to prepare a 25 w / v % PLGA spinning solution; then take a certain mass of sodium selenite (Na2SeO3) powder (the mass ratio of sodium selenite to PLGA is 7.75:92.25) and add it to the PLGA spinning solution, and continuously stir on a magnetic stirrer for 3 h to obtain a selenium-containing spinning solution.
[0058] (2)Electrospinning
[0059] Connect the selenium-containing spinning solution obtained in step (1) to a spinning nozzle. The parameters of the electrospinning equipment are set as follows: the distance between the nozzle and the receiving drum is 15 cm, the spinning speed is 3 mL / h, the voltage is 22 kV, the ambient temperature inside the electrospinning equipment is 25 °C, the bottom of the drum rotates at a low speed and is covered with aluminum foil to collect fibers. After spinning, it is dried in a vacuum drying oven to volatilize the residual solvent. The drying temperature is 60 °C and the time is 24 h to obtain a selenium-containing electrospun fiber membrane.
[0060] (3) Preparation of macrophage cell membranes
[0061] The macrophage cell membrane was extracted from macrophages by the repeated freeze-thaw method. The specific steps are as follows: suspend macrophages in PBS buffer, and after three freeze-thaw cycles (-80 °C freezing, 37 °C thawing), then centrifuge at 10000 rpm for 10 min to obtain the macrophage cell membrane.
[0062] (4) Preparation of biomimetic selenium-doped fiber membrane
[0063] Mix the macrophage cell membrane obtained in step (3) and the selenium-containing electrospun fiber obtained in step (2) at a mass ratio of 1:5, place it in an ultrasonic oscillator, and oscillate at a frequency of 30 kHz for 30 min to ensure that the macrophage cell membrane uniformly wraps the fiber surface to obtain a biomimetic selenium-doped fiber membrane.
[0064] Comparative Example 1:
[0065] A method for preparing a PLGA fiber membrane, comprising the following steps:
[0066] (1) Preparation of selenium-containing spinning solution:
[0067] First, weigh PLGA (polylactic acid-glycolic acid copolymer) using a precision analytical balance. Using dichloromethane (DCM) and dimethylformamide (DMF) as solvents in a volume ratio of 2.5:1, dissolve PLGA in the mixed solvent, and place it on a magnetic stirrer and stir continuously for 12 hours to prepare a 20 w / v % PLGA spinning solution.
[0068] (2) Electrospinning
[0069] Connect the PLGA spinning solution obtained in step (1) to a spinning nozzle. The parameters of the electrospinning equipment are set as follows: the distance between the nozzle and the receiving drum is 18 cm, the spinning speed is 3 mL / h, the voltage is 22 kV, the ambient temperature inside the electrospinning equipment is 25 °C, the bottom of the drum rotates at a low speed and is covered with aluminum foil to collect fibers. After spinning, it is dried in a vacuum drying oven to volatilize the residual solvent. The drying temperature is 60 °C and the time is 24 h to obtain a PLGA fiber membrane (PLGA), and the SEM image is as Figure 2 shown.
[0070] Comparative Example 2:
[0071] A preparation method of a selenium-containing fiber membrane, comprising the following steps:
[0072] (1) Preparation of selenium-containing spinning solution:
[0073] First, weigh PLGA (poly(lactic-co-glycolic acid)) using an analytical balance. Using dichloromethane (DCM) and dimethylformamide (DMF) in a volume ratio of 2.5:1 as the solvent, dissolve PLGA in the mixed solvent, and place it on a magnetic stirrer and continuously stir for 12 hours to prepare a 20 w / v % PLGA spinning solution. Then, take a certain mass of sodium selenite (Na2SeO3) powder (the mass ratio of sodium selenite to PLGA is 7.75:92.25) and add it to the PLGA spinning solution, and continuously stir on a magnetic stirrer for 3 h to obtain a selenium-containing spinning solution.
[0074] (2) Electrospinning
[0075] Connect the selenium-containing spinning solution obtained in step (1) to a spinning nozzle. The parameters of the electrospinning equipment are set as follows: the distance between the nozzle and the receiving drum is 18 cm, the spinning speed is 3 mL / h, the voltage is 22 kV, the ambient temperature inside the electrospinning equipment is 25 °C, and the fiber is collected by covering the bottom of the drum with aluminum foil. After spinning, dry and volatilize the residual solvent in a vacuum drying oven, the drying temperature is 60 °C, and the time is 24 h to obtain a selenium-containing fiber membrane (Se@Fiber). The SEM image is as shown in Figure 3 shown.
[0076] Test Example 1: In vitro inflammatory factor clearance experiment
[0077] Co-culture the biomimetic selenium-doped fiber membrane (MSe@Fiber) prepared in Example 1, the PLGA fiber membrane (PLGA) prepared in Comparative Example 1, and the selenium-containing fiber membrane (Se@Fiber) prepared in Comparative Example 2 with a high-glucose medium containing inflammatory factors (IL-1β and TNF-α) (IL-1β concentration: 100 pg / mL, TNF-α concentration: 220 pg / mL), and set a blank control group (control) without adding a fiber membrane. Detect the concentration change of inflammatory factors in the medium by ELISA.
[0078] The results are as shown in Figure 4As shown in the figure, the results show that the average IL-1β concentrations of the control group, PLGA group, Se@Fiber group, and MSe@Fiber group were 95.2 pg / mL, 92.9 pg / mL, 92.3 pg / mL, and 63.3 pg / mL, respectively; the average TNF-α concentrations were 211.7 pg / mL, 209.3 pg / mL, 207.4 pg / mL, and 128.1 pg / mL, respectively. Data analysis shows that the clearance effect of the MSe@Fiber group on IL-1β reached 33.5%, which was significantly lower than that of Comparative Example 1 and Comparative Example 2; at the same time, the clearance effect of the MSe@Fiber group on TNF-α reached 39.5%, which was also significantly lower than that of Comparative Example 1 and Comparative Example 2, while the fiber membranes prepared in Comparative Example 1 and Comparative Example 2 did not have such an effect. The above results prove that the bionic selenium-doped fiber membrane (MSe@Fiber) prepared by the present invention exhibits significant inflammatory factor clearance ability in in vitro experiments, and its surface macrophage cell membrane wrapping has significant superiority in regulating the inflammatory microenvironment.
[0079] Test Example 2: Macrophage polarization regulation experiment
[0080] The bionic selenium-doped fiber membrane (MSe@Fiber) prepared in Example 1, the PLGA fiber membrane (PLGA) prepared in Comparative Example 1, and the selenium-containing fiber membrane (Se@Fiber) prepared in Comparative Example 2 were co-cultured with macrophages under inflammatory stimulation, and the macrophage density was 4×10 4 / cm 2 , inflammation stimulation method: stimulate with a medium containing 1 μg / mL lipopolysaccharide for 12 hours, medium preparation: containing 89% high-glucose medium, 10% fetal bovine serum, 1% penicillin-streptomycin, the above percentages are all volume fractions, and a blank control group (control) without adding a fiber membrane was set. After culturing for 48 h, the expression levels of macrophage inflammation differentiation markers (IL-1β and IL-6) and macrophage repair-promoting differentiation markers (Arg-1 and CD206) were detected by qPCR.
[0081] The experimental results are as Figure 5As shown. The results showed that, compared with the comparative example and the blank control group, the biomimetic selenium-doped fiber membrane (MSe@Fiber) prepared in Example 1 of the present invention could significantly reduce the expression of IL-1β and IL-6, and the reduction amplitude was significantly higher than that of the Se@Fiber group in Comparative Example 2; at the same time, the expressions of Arg-1 and CD206 in the MSe@Fiber group were significantly increased, and the increase amplitude was better than that of the Se@Fiber group. There was no significant difference between the PLGA group in Comparative Example 1 and the blank control group. The results indicated that the biomimetic selenium-doped fiber membrane (MSe@Fiber) prepared in the present invention could significantly inhibit the inflammatory differentiation of macrophages and promote their differentiation towards repair promotion, further demonstrating its superiority in regulating macrophage polarization.
[0082] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.
Claims
1. A preparation method of a bionic selenium-doped fiber membrane, characterized in that, It includes the following steps: (1) First, mix poly(lactic-co-glycolic acid) and sodium selenite in a solvent and stir to prepare a spinning solution, then perform electrospinning, and finally dry to obtain selenium-containing electrospun fibers; (2) Mix the selenium-containing electrospun fibers obtained in step (1) with macrophage membranes and perform ultrasonic oscillation to obtain; In step (1), the concentration of poly(lactic-co-glycolic acid) in the spinning solution is 10 w / v % - 30 w / v %; the mass ratio of poly(lactic-co-glycolic acid) to sodium selenite is (90 - 95):(5 - 10); In step (2), the mass ratio of the selenium-containing electrospun fibers to macrophage membranes is (5 - 20):
1.
2. The preparation method of the biomimetic selenium-doped fiber membrane according to claim 1, wherein In step (1), the solvent is a mixed solvent composed of dichloromethane and dimethylformamide; the volume ratio of dichloromethane to dimethylformamide is (1 - 5):
1.
3. The preparation method of the biomimetic selenium-doped fiber membrane according to claim 1, wherein The conditions for electrospinning in step (1) are: the distance between the nozzle and the receiving roller is 15 - 20 cm, the spinning speed is 1 - 5 mL / h, the spinning voltage is 20 - 25 kV, and the spinning environment temperature is 20 - 30 °C; the drying temperature is 50 - 70 °C and the time is 20 - 30 h.
4. The preparation method of the bionic selenium-doped fiber membrane according to claim 1, characterized in that The macrophage membranes in step (2) are prepared by the following method: First, suspend macrophages in phosphate buffer solution, then perform freeze-thaw cycle treatment, and finally perform centrifugal separation to obtain.
5. The preparation method of the bionic selenium-doped fiber membrane according to claim 4, wherein, The conditions for the freeze-thaw cycle treatment are: first freeze at -90~-70 °C, then thaw at 35 - 40 °C, and repeat freezing and thawing 2 - 5 times; the centrifugal rotation speed is 8000 - 12000 rpm and the time is 5 - 15 min.
6. The preparation method of the bionic selenium-doped fiber membrane according to claim 1, wherein, In step (2), the frequency of ultrasonic oscillation is 30 - 50 kHz and the time is 10 - 60 min.
7. A bionic selenium-doped fiber membrane, characterized in that, Prepared by the preparation method according to any one of claims 1 - 6.
8. Use of the biomimetic selenium-doped fiber membrane according to claim 7 in the preparation of medical devices or drugs for treating infectious bone defects.