A method for synergistically treating erucamide wastewater by Arthrobacter and Bacillus megaterium based on staged aeration regulation

Abstract

The present invention discloses a method for synergistically treating erucamide wastewater by Arthrobacter and Bacillus megaterium based on staged aeration regulation, belonging to the field of environmental microbiological technology. In the present invention, pre-cultured Arthrobacter and Bacillus megaterium are prepared into a composite bacterial agent and inoculated into erucamide wastewater, and a biological enhancement treatment is implemented by adopting a staged aeration regulation strategy: in stage I, high aeration conditions are adopted to activate the rapid proliferation of bacteria and induce the expression of amidase, and in stage II, the aeration conditions are adjusted to low aeration conditions to optimize the anoxic environment for deamidation reaction. The present invention realizes the spatio-temporal synergistic effect of two functional bacteria by regulating the dissolved oxygen concentration, that is, Bacillus megaterium preferentially degrades ammonia nitrogen pollutants, and Arthrobacter specifically catalyzes the cleavage of the amide bond of erucamide, and their metabolic pathways form a complementarity; this process conforms to the principle of green chemistry and no secondary pollutants are generated, providing a new efficient and sustainable biological treatment approach for refractory organic wastewater containing erucamide.

Description

Technical Field

[0001] The invention belongs to the technical field of environmental microorganisms, and in particular relates to a method for treating erucamide wastewater by cooperating with Arthrobacter and Bacillus megaterium based on staged aeration regulation. Background Art

[0002] In contemporary industrial production, erucamide, as a key fine chemical raw material, is widely used in the plastics, ink and coating industries. In the plastics industry, it acts as a lubricant and anti-sticking agent, significantly improving the quality and processing performance of plastic products; in the ink industry, it enhances the fluidity and dispersibility of inks, ensuring the quality of printed products; in the coating field, it enhances the wear resistance and scratch resistance of coatings, and is used in high-end coating products. However, the expansion of erucamide production has led to increasingly serious wastewater pollution problems.

[0003] The composition of erucamide wastewater is complex, containing high concentrations of long-chain fatty acids, amide substances and additives, and is difficult to degrade naturally. Direct discharge will lead to eutrophication of water bodies, destroy the ecological balance, and affect aquatic life; at the same time, it will destroy the physical and chemical properties of the soil, affect soil fertility and microbial communities, and threaten the stability of the ecosystem. Traditional physical treatment methods such as sedimentation and filtration can remove suspended particles and impurities, but have limited effects on the removal of dissolved organic matter and nutrients, and have high energy consumption. Chemical treatment methods such as chemical oxidation and coagulation sedimentation can decompose and remove pollutants, but require a large amount of chemical reagents, are costly and easily cause secondary pollution.

[0004] Biological treatment methods have attracted much attention due to their low cost and environmental friendliness. However, traditional biological treatment methods, such as the activated sludge method, have the following defects in treating erucamide wastewater:

[0005] 1. Traditional biological methods are inefficient: For example, the Chinese patent CN105198112A (publication date: 2015.12.30) is not efficient enough in degrading long-chain fatty acids and amides in erucamide wastewater, and high concentrations of pollutants and special chemical compositions may inhibit microbial growth and metabolism, resulting in poor treatment effects. The activated sludge method has a COD (chemical oxygen demand) removal rate of less than 65%, and ammonia nitrogen removal rate of less than 55%;

[0006] 2. Poor tolerance to high salt: When the salinity of wastewater is greater than 2% NaCl, the activity of microorganisms is inhibited and the COD removal rate drops to less than 50%;

[0007] 3. The treatment cycle is as long as 72-96 hours, the equipment occupies a large area, and the construction cost is increased. It has weak adaptability to water quality and water volume shocks and is difficult to cope with fluctuations. Moreover, it is easy to produce a large amount of residual sludge, which is difficult to dispose of later and the cost is quite high.

[0008] In addition, with the increasingly strict environmental protection requirements and the continuous improvement of wastewater discharge standards, conventional biological treatment methods are no longer able to meet the discharge requirements.

[0009] Therefore, it is urgent to develop an efficient, low-cost and environmentally friendly method for treating erucamide wastewater. This is not only an urgent need to solve the current environmental problems, but also the key to promoting the sustainable development of related industries, and has important practical significance for achieving the coordinated development of economy and environment. Summary of the Invention

[0010] The technical problem to be solved by the present invention is to provide a method for synergistically treating erucamide wastewater by Arthrobacter and Bacillus megaterium based on staged aeration regulation, which significantly improves the treatment efficiency of erucamide wastewater, reduces the treatment cost, ensures that the wastewater can stably meet the discharge standards, and realizes the coordinated development of environmental protection and economic benefits.

[0011] To solve the above technical problems, the technical solution adopted by the present invention is as follows:

[0012] A method for synergistically treating erucamide wastewater by Arthrobacter and Bacillus megaterium based on staged aeration regulation, comprising the following steps:

[0013] 1) Strain cultivation: Cultivate Arthrobacter strain and Bacillus megaterium strain;

[0014] 2) Bacterial agent compounding and inoculation: Compound and inoculate the Arthrobacter and Bacillus megaterium strains cultured in step 1) into erucamide wastewater;

[0015] 3) Staged biological enhancement treatment;

[0016] 4) Subsequent treatment: After the biological enhancement treatment is completed, perform solid-liquid separation, and the supernatant is adsorbed by activated carbon and filtered through a membrane.

[0017] In step 1), the process of culturing Arthrobacter strain is as follows: Use LB medium, add 0.1 wt% erucamide to induce enzyme activity, and culture at 30 °C and 150 rpm with shaking until OD 600 reaches 2.0.

[0018] In step 1), the process of culturing Bacillus megaterium strain is as follows: Use an optimized fermentation medium, culture at 37 °C and 200 rpm with shaking for 18 h until the spore formation rate ≥ 90%.

[0019] The preparation of the LB medium: 10 g / L of tryptone, 5 g / L of yeast extract, 10 g / L of NaCl, pH 7.0, and the sterilization conditions are 121 °C and 20 min.

[0020] Preparation of the optimized fermentation medium: 20 g / L glucose and 1 g / L dipotassium hydrogen phosphate, pH 7.0, sterilization conditions: 121 °C, 20 min.

[0021] In step 2), the mixing volume ratio of Arthrobacter and Bacillus megaterium is 3 - 1:1 - 3.

[0022] In step 2), the volume of erucamide wastewater is 10 - 100 L.

[0023] In step 2), the COD concentration of erucamide wastewater is 1000 - 3000 mg / L.

[0024] In step 3), the process of staged bioaugmentation for wastewater treatment is as follows: two-stage reaction: Stage I: aeration rate 2.5 L / min, dissolved oxygen DO maintained at 5.0 - 6.0 mg / L; Stage II: reduce aeration to 1.0 L / min, dissolved oxygen DO controlled at 1.5 - 2.0 mg / L.

[0025] Compared with the prior art, the present invention has the following advantages:

[0026] (1) The present invention utilizes the ammonia nitrogen degradation ability of Bacillus megaterium (ammonia nitrogen removal rate ≥ 94.7%) and the specific degradation function of Arthrobacter for erucamide. Through high aeration in Stage I to activate rapid proliferation of bacteria and induction of amidase, and low aeration in Stage II to optimize the anoxic deamidation reaction conditions, synergistic metabolism is achieved.

[0027] (2) The present invention regulates aeration in stages: in Stage I (0 - 12 h), aeration rate 2.5 L / min, COD degradation 40% - 50%; in Stage II (12 - 48 h), aeration rate 1.0 L / min, erucamide degradation rate increased to ≥ 85%, COD removal rate ≥ 80%, ammonia nitrogen synchronous degradation rate ≥ 75%, applicable to high-salt (≤ 5% NaCl) wastewater, effectively ensuring stable discharge of wastewater up to standard.

[0028] (3) The treatment method of the present invention has low cost, and the microbial agent can be reused 3 - 5 times. When the volume ratio of the seed solution of Arthrobacter and Bacillus megaterium is 3:1, the COD removal rate is the highest (88.4%), and the microbial agent can be reused 5 times, with a 40% cost reduction. The entire treatment process is green and environmentally friendly, without generating secondary pollution, and has significant economic and environmental benefits, providing a sustainable innovative solution for the treatment of erucamide wastewater. Detailed implementation manners

[0029] The following further clarifies the present invention in conjunction with specific embodiments. The embodiments are implemented on the premise of the technical solution of the present invention. It should be understood that these embodiments are only used to illustrate the present invention and not to limit the scope of the present invention.

[0030] In the following examples, COD was tested by the potassium dichromate method (HJ828 - 2017).

[0031] In the following examples, Arthrobacter sp. (deposit number CGMCC NO: 23463) is described in the Chinese invention patent with publication number CN114437979A: Arthrobacter capable of degrading erucamide and its obtaining method, cultivation method and application; Bacillus megaterium (deposit number CCTCC NO: M2012381) is described in the Chinese invention patent with publication number CN110194722A: A method for separating and extracting erucamide using Bacillus megaterium.

[0032] Example 1

[0033] Preparation of compound bacterium agent

[0034] (1) Strain cultivation

[0035] Arthrobacter sp. (deposit number CGMCC NO: 23463): Using LB liquid medium (tryptone 10 g / L, yeast extract 5 g / L, NaCl 10 g / L, pH 7.0, sterilization conditions: 121 °C, 20 min), adding 0.1% (w / w) erucamide as an enzyme activity inducer, and culturing with shaking at 30 °C, 150 rpm until OD 600 reaches 2.0 (about 24 h is required, and the cell concentration ≥ 1×10 8 CFU / mL) at this time.

[0036] Bacillus megaterium (deposit number CCTCC NO: M2012381): Using an optimized fermentation medium (glucose 20 g / L, dipotassium hydrogen phosphate 1 g / L, pH 7.0, sterilization conditions: 121 °C, 20 min), culturing at 37 °C, 200 rpm for 18 h, and the spore formation rate ≥ 90% (refer to the modified malachite green staining method in "GB / T 4789.2 - 2016 Microbiological examination of foods - Determination of total number of colonies").

[0037] (2) Mix the seed solutions of Arthrobacter sp. and Bacillus megaterium in a volume ratio of 3:1 to obtain a compound bacterium agent. The activity of the bacterium agent was verified by measuring the OD 600 after mixing and the 24 - hour degradation rate (≥ 50%) of the simulated wastewater (COD 1000 mg / L).

[0038] Example 2

[0039] (1)Phased strengthening treatment

[0040] Wastewater parameters: The volume of wastewater is 50 L, the initial COD concentration is 1500 mg / L, the ammonia nitrogen concentration is 120 mg / L, the salinity is 2% NaCl, and the pH is 8.3.

[0041] Inoculation: The compound bacterium agent prepared in Example 1 was inoculated into the erucamide wastewater at an inoculation amount of 5% of the wastewater volume (i.e., 2.5 L of the bacterium agent was inoculated into 50 L of wastewater); the pH was adjusted to 7.0 ± 0.2 (adjusted using NaOH / HCl), and soluble starch (food grade) was added as a co-metabolic carbon source (C / N = 15:1).

[0042] Phased strengthening treatment: Stage I (0 - 12 h): Control the aeration volume at 2.5 L / min, maintain the dissolved oxygen (DO) at 5.0 - 6.0 mg / L, the temperature at 30 ± 1 °C, the COD degradation rate at 48.7%, the erucamide degradation rate at 26.7% (HPLC method, GB / T 32952 - 2016), and the ammonia nitrogen removal rate at 74.7% (Nessler reagent method); Stage II (12 - 48 h): Reduce the aeration volume to 1.0 L / min, control the DO at 1.5 - 2.0 mg / L, the temperature at 30 ± 1 °C, the COD degradation rate at 88.4%, the erucamide degradation rate increased to 86.7%, and the synchronous ammonia nitrogen removal rate at 90.2%. The effluent effect results are shown in Table 1.

[0043] (2)Subsequent treatment

[0044] Solid-liquid separation: After the biological strengthening treatment, the wastewater was subjected to solid-liquid separation using centrifugation technology, centrifuged at 4000 rpm for 10 min, and the cell recovery rate was ≥95% (wet weight method).

[0045] Advanced treatment: The supernatant obtained by separation was further treated by activated carbon adsorption (particle size 200 mesh, dosage 2 g / L) and high-precision membrane filtration (0.22 μm PVDF) to ensure the effective removal of residual organic matter, pigments, fine particles, and dissolved pollutants in the wastewater.

[0046] Table 1 Effluent effect

[0047]

[0048] The results in Table 1 show the following conclusions:

[0049] (1)The synergistic effect of phased aeration is obvious

[0050] In this embodiment, through the oxygen gradient regulation of Stage I (aerobic phase, DO 5.0 - 6.0 mg / L) and Stage II (micro-aerobic phase, DO 1.5 - 2.0 mg / L), the metabolic function complementarity of the two types of bacteria is achieved:

[0051] Stage I: The high dissolved oxygen environment significantly activates the amidase activity of Arthrobacter. The COD degradation rate is 48.7% (0 - 12 h), and the erucamide degradation rate is 26.7%, laying a carbon source foundation for the subsequent deamidation reaction.

[0052] Stage II: After reducing the aeration volume, the ammonia oxidation function of Bacillus megaterium dominates. The ammonia nitrogen degradation rate jumps from 74.7% in Stage I to 90.2% of the total degradation rate. At the same time, the erucamide degradation rate increases to 86.7%, reflecting the sequential switching of the bacterial community functions driven by the oxygen environment.

[0053] (2) The pollutant removal efficiency fully meets the standards

[0054] COD: It drops from 1500 mg / L to 68.5 mg / L, better than the first-class standard of the Integrated Wastewater Discharge Standard (GB 8978 - 2002) (COD ≤ 100 mg / L).

[0055] Ammonia nitrogen: The concentration after advanced treatment is 0.96 mg / L, meeting the requirements of the most stringent Class III water body of the Surface Water Environment Quality Standard (GB3838 - 2002) (ammonia nitrogen ≤ 1.0 mg / L).

[0056] Erucamide: The final concentration is lower than the detection limit (0.5 mg / L), achieving complete harmlessness.

[0057] (3) Outstanding technical and economic advantages

[0058] Optimized treatment cycle: The total time-consuming is 48 h (including advanced treatment), with an efficiency improvement of 33% - 50% compared to the traditional activated sludge process (72 - 96 h). Moreover, no additional chemical agents need to be added, and there is no risk of secondary pollution.

[0059] (4) Verification of innovative technical features

[0060] Necessity of oxygen gradient regulation: Comparative experiments show that (see Comparative Example 1), the COD removal rate of the constant DO treatment (5.0 or 1.5 mg / L) is only 68.3% - 79.5%, significantly lower than 88.4% of the staged aeration, proving the irreplaceability of the dynamic regulation of the oxygen environment for the cooperation of the bacterial community.

[0061] Process adaptability: This solution still maintains high treatment ability for high-salt (2% NaCl) and complex pollutant (COD / ammonia nitrogen / amide coexistence) wastewater, breaking through the environmental tolerance bottleneck of traditional biological methods.

[0062] Example 3

[0063] The difference between this example and Example 2 is as follows:

[0064] Wastewater parameters: The volumes of three groups of erucamide wastewater are 10 L, 50 L, and 100 L respectively. The initial COD concentration is 1500 ± 50 mg / L, the ammonia nitrogen concentration is 100 ± 5 mg / L, the salinity is 2% NaCl, and the pH is 8.5 ± 0.3.

[0065] The COD degradation rate in Stage I is 45% - 48%; the erucamide degradation rate in Stage II is ≥ 85% (HPLC method, GB / T32952 - 2016), and the ammonia nitrogen removal rate is ≥ 80% (Nessler reagent method, HJ535 - 2009). See Table 2 for the detailed effluent results.

[0066] Table 2 Effluent Results

[0067]

[0068] The results in Table 2 show that within the range of 10 - 100 L, the removal rate of chemical oxygen demand (COD) is ≥ 83.6%, which is more than 20% higher than that of the traditional activated sludge method. The ammonia nitrogen removal rate is ≥ 80.5%, and the energy consumption per unit of wastewater is significantly reduced, from 1.8 kW·h / m 3 to 1.3 kW·h / m 3 , with a decrease of 27.8%. In addition, the recovery rate of the bacterial agent always remains above 90%. These data fully demonstrate that this method shows good treatment effects at different scales. It has strong scale adaptability, and the treatment process has good economy and stability, and has the potential for large-scale application.

[0069] Example 4

[0070] The difference between this example and Example 2 is as follows:

[0071] Wastewater parameters: The COD concentrations of three groups of erucamide wastewater are different. The main parameter indicators of the wastewater are shown in Table 3 below.

[0072] Table 3 Main Parameter Indicators of Wastewater

[0073]

[0074] The COD degradation rate in Stage I is 42% - 48%. See Table 4 for the degradation efficiency of erucamide and ammonia nitrogen, and see Table 5 for the COD treatment effect results of the wastewater.

[0075] Table 4 Degradation Efficiency of Erucamide and Ammonia Nitrogen

[0076]

[0077] Table 5 Treatment Effect of Wastewater COD

[0078]

[0079] The results in Table 4 and Table 5 show the following conclusions:

[0080] (1) Obvious improvement in treatment efficiency

[0081] Degradation efficiency: In the complex wastewater system with COD of 1000 - 3000 mg / L and salinity ≤ 3% NaCl, the degradation rate of erucamide ≥ 85.6%, the COD removal rate ≥ 84.5%, and the synchronous ammonia nitrogen removal rate ≥ 80.3%. Compared with the traditional activated sludge method (COD removal rate 65% and ammonia nitrogen removal rate 55%), it is improved by 20% - 40%.

[0082] Period optimization: The treatment period is shortened to 48 - 60 h (the traditional method requires more than 72 h), and the efficiency is improved by 20% - 40%.

[0083] Salt tolerance breakthrough: Under the condition of 3% NaCl high salinity, the activity of the bacterial community is not significantly inhibited, breaking through the normal salinity tolerance range of the traditional biological method (usually < 2% NaCl).

[0084] (2) Economic advantages

[0085] The staged aeration strategy reduces the energy consumption to 1.3 - 1.8 kW·h / ton. Compared with the conventional bioreactor (2.5 - 3.0 kW·h / ton), the energy saving effect reaches 40% - 56%.

[0086] (3) Environmental benefits

[0087] Green process: No chemical agents need to be added throughout the process, avoiding the generation of chemical sludge and the risk of secondary pollution. At the same time, compared with the chemical treatment method, this process achieves significant carbon emission reduction.

[0088] (4) Embodiment of technological innovation

[0089] Synergistic mechanism of strains: Driven by staged aeration, the amidase activity of Arthrobacter sp. (CGMCC 23463) and the fatty acid degradation function of Bacillus megaterium (CCTCC M2012381) form metabolic complementarity, and the synergistic efficiency is improved by 30% - 50%.

[0090] Process adaptability: By dynamically adjusting the aeration volume (from 2.5 L / min to 1.0 L / min) and dissolved oxygen concentration (from 5.0 mg / L to 1.5 mg / L), the orderly and efficient degradation of carbon and nitrogen pollutants is achieved, effectively improving the problem of biological inhibition in the high-salt environment.

[0091] Example 5

[0092] The difference between this example and Example 2 is as follows:

[0093] Wastewater parameters: The volume of three groups of erucamide wastewater is 50 L, the COD concentration is 2000 ± 30 mg / L, the ammonia nitrogen concentration is 105 ± 5 mg / L, the salinity is 3% NaCl, and the pH is 8.4 ± 0.2.

[0094] Inoculation: The compound bacterium agent obtained by mixing the Arthrobacter and Bacillus megaterium seed solutions in Example 1 at volume ratios of 3:1, 1:1, and 1:3 respectively was inoculated into the erucamide wastewater.

[0095] For the degradation efficiency of erucamide and ammonia nitrogen, see Table 6 in detail. The treatment effect of wastewater COD is shown in Table 7.

[0096] Table 6 Degradation efficiency of erucamide and ammonia nitrogen

[0097]

[0098] Table 7 Treatment effect of wastewater COD

[0099]

[0100] The results in Table 6 and Table 7 show that at three different ratios, the composite bacterial community all showed good treatment effects, indicating that this method is feasible within a certain range of bacterial species ratios. Among them, at the compound ratio of 3:1, the COD removal rate is the highest, which is 26.9% higher than that of a single bacterial species, fully reflecting the good synergistic effect between the two bacterial species.

[0101] Example 6

[0102] The difference between this example and Example 2 is as follows:

[0103] Wastewater parameters: After the actual chemical industrial wastewater is treated by traditional anaerobic-aerobic-physical and chemical methods, the water quality characteristics are as follows: the volume is 10 L, the erucamide concentration is 21.7 mg / L, the COD concentration is 158 mg / L, the ammonia nitrogen concentration is 16.4 mg / L, the pH is 8.2, and the salinity is 3%.

[0104] Inoculation: Adjust the pH of the wastewater to 7.5 ± 0.2.

[0105] For the degradation efficiency of erucamide and ammonia nitrogen, see Table 8 in detail. The treatment effect of wastewater COD is shown in Table 9, and the change of the degradation enzyme system is shown in Table 10.

[0106] Table 8 Degradation efficiency of erucamide and ammonia nitrogen

[0107]

[0108] Table 9 COD treatment effect of wastewater

[0109]

[0110] Table 10 Changes in the degradation enzyme system

[0111]

[0112] The results in Table 8 and Table 9 show that for chemical wastewater containing multiple pollutants (COD / ammonia nitrogen / amide), after traditional biological treatment, the COD removal rate is still as high as 60.5%, indicating that this technology has good adaptability to complex wastewater, produces no chemical sludge, and can achieve carbon emission reduction.

[0113] The results in Table 10 show that the introduction of Arthrobacter and Bacillus megaterium greatly increases the abundance of the microbial degradation enzyme system, which is beneficial to the removal of erucamide and refractory substances. Among them, peroxidase helps to maintain a redox environment conducive to pollutant degradation, accelerates the transformation of organic matter and the removal process of pollutants such as nitrogen, indicating that the activated sludge in the invention group can degrade organic pollutants more efficiently. Hydrogenase participates in multiple metabolic pathways, including organic matter transformation and energy generation. Its high abundance indicates that microorganisms can utilize substrates more efficiently for energy metabolism and material transformation. The series of enzymes in groups 3.1 and 3.5 show that the invention group has stronger ability to decompose complex organic molecular structures (such as esters, proteins, and nucleic acids), which helps to rapidly degrade the difficult-to-treat organic pollutants in sewage. The series of enzymes in 4.1 and 4.2 indicate that bioaugmentation treatment accelerates the mineralization process of organic matter and enhances the degradation ability of organic pollutants.

[0114] Comparative Example 1

[0115] The difference between this example and Example 2 is as follows:

[0116] Wastewater parameters: The main parameter indicators of the wastewater are shown in Table 11 below.

[0117] Table 11 Main parameter indicators of wastewater

[0118]

[0119] Inoculation: The seed solutions of Arthrobacter and Bacillus megaterium were inoculated into erucamide wastewater at an inoculation amount of 5% of the wastewater volume respectively, and the pH was adjusted to 7.0 ± 0.2.

[0120] Staged bioaugmentation treatment: The control group uniformly adopted an aeration rate of 2.5 L / min throughout the process. In terms of the treatment duration, the control group was 72 - 96 h, while the invention group was 48 h. The bioaugmentation treatment parameters are shown in Table 12.

[0121] Table 12 Bioaugmentation treatment parameters

[0122]

[0123] The treatment effect results of Comparative Example 1 are shown in Table 13.

[0124] Table 13 Treatment effect of Comparative Example 1

[0125]

[0126] The results in Table 13 show that compared with the treatment of single strain, the composite microbial agent has an 8.9% - 20.1% increase in COD removal rate and a 28.1% - 64.3% increase in erucamide degradation rate; compared with the traditional activated sludge process, the composite microbial agent shortens the treatment cycle by 50%, increases the COD removal rate by 23.4%, and increases the erucamide degradation rate by 63.1%, showing a significant synergistic effect.

[0127] The above are only the preferred embodiments of the present invention. It should be pointed out that for those of ordinary skill in the art, without departing from the principle of the present invention, several improvements and refinements can be made, and these improvements and refinements should also be regarded as the protection scope of the present invention.

Claims

1. A method for synergistically treating erucamide wastewater by Arthrobacter and Bacillus megaterium based on staged aeration regulation, characterized in that It includes the following steps: 1) Strain cultivation: Cultivate Arthrobacter sp. and Bacillus megaterium sp.; 2) Bacterial agent compounding and inoculation: Compound and inoculate the Arthrobacter sp. and Bacillus megaterium sp. cultivated in step 1) into erucamide wastewater; 3) Stage-by-stage biological enhancement treatment: Through high aeration in Stage I to activate rapid proliferation of bacteria and induction of amidase, and low aeration in Stage II to optimize the anoxic deamidation reaction conditions, synergistic metabolism is achieved; 4) Subsequent treatment: After the biological enhancement treatment, solid-liquid separation is carried out, and the supernatant is adsorbed by activated carbon and filtered through a membrane; The preservation number of the Arthrobacter sp. is CGMCC NO: 23463; the preservation number of the Bacillus megaterium sp. is CCTCC NO: M2012381.

2. The method for synergistically treating erucamide wastewater by Arthrobacter and Bacillus megaterium based on staged aeration regulation according to claim 1, characterized in that: In the step 1), the culture process of the Arthrobacter strain is as follows: Using LB medium, adding 0.1 wt% erucamide to induce enzyme activity, and culturing with shaking at 30 °C and 150 rpm until the OD 600 reaches 2.

0.

3. The method for synergistically treating erucamide wastewater by Arthrobacter and Bacillus megaterium based on staged aeration regulation according to claim 1, wherein: In step 1), the cultivation process of the Bacillus megaterium sp. is: Use an optimized fermentation medium, shake-culture at 37 °C and 200 rpm for 18 h until the spore formation rate ≥ 90%.

4. The method for synergistically treating erucamide wastewater by Arthrobacter and Bacillus megaterium based on staged aeration regulation according to claim 2, characterized in that: The preparation of the LB medium: 10 g / L tryptone, 5 g / L yeast extract, 10 g / L NaCl, pH 7.0, sterilization conditions: 121 °C, 20 min.

5. The method for synergistically treating erucamide wastewater by Arthrobacter and Bacillus megaterium based on staged aeration regulation according to claim 3, wherein: The preparation of the optimized fermentation medium: 20 g / L glucose and 1 g / L dipotassium hydrogen phosphate, pH 7.0, sterilization conditions: 121 °C, 20 min.

6. The method for synergistically treating erucamide wastewater by Arthrobacter and Bacillus megaterium based on staged aeration regulation according to claim 1, characterized in that: In step 2), the mixing volume ratio of Arthrobacter sp. and Bacillus megaterium sp. is 3~1:1~3.

7. The method for synergistically treating erucamide wastewater by Arthrobacter and Bacillus megaterium based on staged aeration regulation according to claim 1, wherein: In step 2), the volume of the erucamide wastewater is 10~100 L.

8. The method for synergistically treating erucamide wastewater by Arthrobacter and Bacillus megaterium based on staged aeration regulation according to claim 1, wherein: In step 2), the COD concentration of the erucamide wastewater is 1000~3000 mg / L.

9. The method for synergistically treating erucamide wastewater by Arthrobacter and Bacillus megaterium based on staged aeration regulation according to claim 1, wherein: In step 3), the process of stage-by-stage biological enhancement treatment of wastewater is: Two-stage reaction: Stage I: Aeration rate 2.5 L / min, dissolved oxygen DO is maintained at 5.0 - 6.0 mg / L; Stage II: Reduce aeration to 1.0 L / min, dissolved oxygen DO is controlled at 1.5 - 2.0 mg / L.

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