Desiccated coconut feed prepared based on lactobacillus fermentation and preparation method of desiccated coconut feed

Abstract

The invention belongs to the technical field of feed preparation, and particularly relates to shredded coconut stuffing feed prepared based on lactobacillus fermentation and a preparation method of the shredded coconut stuffing feed. According to the invention, the high-content manganese sulfate and the low-content dipotassium phosphate are used for expanding culture of the compound lactic acid bacteria stock seeds, so that enzymes in microorganisms are activated, metabolic reaction of the microorganisms can be promoted, fermentation efficiency is improved, growth and metabolism of the microorganisms can be influenced, and composition and properties of fermentation products can be changed; the shredded coconut stuffing feed prepared from the shredded coconut stuffing is good in palatability and high in nutritional value, not only can improve meat flavor and promote animal growth, but also can reduce ammonia emission, and is more environmentally friendly.

Description

Technical Field

[0001] The present invention belongs to the technical field of feed preparation, and particularly relates to a coconut flake feed prepared by lactic acid bacteria fermentation and a preparation method thereof. Background Art

[0002] China is the world's largest producer and consumer of industrial feed, and the feed preparation industry is large-scale and continuously growing. However, with the improvement of the understanding of the functions of feed raw materials and the development of the downstream aquaculture industry, refined feeds are constantly improving. At the same time, factors such as low-carbon environmental protection, meat quality improvement, and supplementation of functional components also need to be considered during the feed preparation process to continuously meet the development needs of the market.

[0003] Coconut flakes are made by cutting high-quality, fresh, thick coconut meat into shreds or grinding it into powder and then drying it. It is rich in nutritional value and can be used to prepare feed. For example, in Chinese Patent CN 111713598 A, a mixture of coconut flakes and soybean meal is used as a fermentation substrate, and a fermented coconut flake biological feed is obtained through two-step solid-state fermentation. Although the fermented coconut flake feed has good palatability, the preparation process is relatively cumbersome, which is not conducive to large-scale production and nutrition. Summary of the Invention

[0004] The purpose of the present invention is to provide a coconut flake feed prepared by lactic acid bacteria fermentation and a preparation method thereof. The preparation process is simple, which can not only improve the meat flavor, promote animal growth, but also reduce ammonia emissions, making it more environmentally friendly.

[0005] The present invention provides a preparation method of a coconut flake feed, comprising the following steps:

[0006] Inoculate the compound lactic acid bacteria original strain into a culture solution for propagation culture to obtain a compound lactic acid bacteria expanded culture solution;

[0007] Mix the compound lactic acid bacteria expanded culture solution with coconut flakes and perform anaerobic fermentation to obtain a coconut flake feed;

[0008] The compound lactic acid bacteria original strain includes: Lactobacillus plantarum, Lactobacillus acidophilus, Bifidobacterium adolescentis, and Saccharomyces cerevisiae;

[0009] The culture solution includes: manganese sulfate and dipotassium hydrogen phosphate.

[0010] Preferably, the culture solution uses water as a solvent and includes the following components: 40-50 g / L of brown sugar, 10-20 g / L of milk powder, and 10-20 g / L of a composition;

[0011] By mass, the composition includes the following raw materials: 15-20 parts of glucose, 10-12 parts of calcium carbonate, 0.25-0.28 parts of magnesium sulfate, 1-1.2 parts of manganese sulfate, and 0.03-0.05 parts of dipotassium hydrogen phosphate.

[0012] Preferably, the composite lactic acid bacteria stock culture is composed of Lactobacillus plantarum, Lactobacillus acidophilus, Bifidobacterium adolescentis, and Saccharomyces cerevisiae in a mass ratio of 1:0.2 - 0.3:0.4 - 0.6:0.02 - 0.03;

[0013] The effective viable counts of Lactobacillus plantarum, Lactobacillus acidophilus, Bifidobacterium adolescentis, and Saccharomyces cerevisiae are 1×10 8 - 2×10 8 cfu / g.

[0014] Preferably, the mass ratio of the composite lactic acid bacteria stock culture to the culture medium is 0.3 - 0.5:10;

[0015] The conditions for the propagation culture include: the temperature is 33 - 35°C, and the time is 44 - 50 h.

[0016] Preferably, during the anaerobic fermentation, the mass ratio of the composite lactic acid bacteria expanded culture solution to the coconut meat is 2 - 2.5:100;

[0017] The effective viable count of the composite lactic acid bacteria expanded culture solution > 5×10 8 cfu / mL.

[0018] Preferably, the conditions for the anaerobic fermentation include: the temperature is 30 - 36°C, and the time is 6 - 8 d.

[0019] Preferably, the present invention provides a coconut meat feed prepared by the preparation method described in the above technical solution.

[0020] The present invention provides the application of the coconut meat feed described in the above technical solution, and the application includes: promoting animal growth, improving meat quality flavor, and reducing ammonia emissions, etc.;

[0021] Preferably, the animals include: one or more of chickens, ducks, geese, pigs, fish, sheep, and cows.

[0022] The present invention provides a method for improving animal meat quality flavor and / or promoting animal growth, including the following steps:

[0023] Feeding the coconut meat feed described in the above technical solution to animals;

[0024] The addition amount of the coconut meat feed is 15wt% - 60wt% of the total amount of the basal diet.

[0025] Beneficial effects:

[0026] The present invention provides a method for preparing coconut meal feed, comprising the following steps: inoculating a composite lactic acid bacteria original strain into a culture medium for propagation culture to obtain a composite lactic acid bacteria expanded culture solution; mixing the composite lactic acid bacteria expanded culture solution with coconut meal and performing anaerobic fermentation to obtain coconut meal feed; the composite lactic acid bacteria original strain includes: Lactiplantibacillus plantarum, Lactobacillus acidophilus, Bifidobacterium adolescentis, and Saccharomyces cerevisiae; the culture medium includes: manganese sulfate and dipotassium hydrogen phosphate. By using manganese sulfate and dipotassium hydrogen phosphate to expand the culture of the composite lactic acid bacteria original strain, the present invention not only activates the enzymes in the microorganisms, can promote the metabolic reactions of the microorganisms, improve the fermentation efficiency, but also affects the growth and metabolism of the microorganisms, changes the composition and properties of the fermentation products; by fermenting it with coconut meal, the biological activity of the secondary metabolites in the coconut meal is significantly improved. Therefore, after fermenting coconut meal with a composite strain containing Lactiplantibacillus plantarum, Lactobacillus acidophilus, Bifidobacterium adolescentis, and Saccharomyces cerevisiae, it is beneficial to improve the palatability and nutritional value of coconut meal feed, and can be used to improve meat quality flavor, promote animal growth, and reduce ammonia emissions. Detailed implementation manners

[0027] In the present invention, unless otherwise specified, the raw materials, methods, and equipment used are all purchased conventionally.

[0028] The present invention provides a method for improving animal meat quality flavor and / or promoting animal growth, comprising the following steps: feeding the coconut meal feed described in the above technical solution to animals; the addition amount of the coconut meal feed is 15 wt% - 60 wt% of the total amount of the basal diet. The addition amount of the coconut meal feed described in the present invention is related to the weight of the animal, that is:

[0029] When the weight of the animal < 25 kg, the addition amount of the coconut meal feed is 15 wt% of the total amount of the basal diet;

[0030] When the weight of the animal ≥ 25 kg and < 50 kg, the addition amount of the coconut meal feed is > 15 wt% and ≤ 25 wt% of the total amount of the basal diet;

[0031] When the weight of the animal ≥ 50 kg and < 75 kg, the addition amount of the coconut meal feed is > 25 wt% and ≤ 35 wt% of the total amount of the basal diet;

[0032] When the weight of the animal ≥ 75 kg and < 100 kg, the addition amount of the coconut meal feed is > 35 wt% and ≤ 40 wt% of the total amount of the basal diet;

[0033] When the weight of the animal ≥ 100 kg, the addition amount of the coconut meal feed is > 40 wt% and ≤ 60 wt% of the total amount of the basal diet.

[0034] The present invention provides the application of the coconut meal feed described in the above technical solution, and the application includes: promoting animal growth, improving meat quality flavor, and reducing ammonia emissions, one or more of them; the improvement of meat quality flavor preferably includes: by improving the types or contents of fatty acids in animal fats, and then achieving the effect of improving meat quality flavor. In a specific embodiment of the present application, the fats preferably include: one or more of lauric acid (C12:0), myristic acid (C14:0), palmitic acid (C16:0), palmitoleic acid (C16:1), stearic acid (C18:0), oleic acid (C18:1, %), linoleic acid (C18:2), and linolenic acid (C18:3), more preferably lauric acid (C12:0), myristic acid (C14:0), palmitic acid (C16:0), palmitoleic acid (C16:1), stearic acid (C18:0), oleic acid (C18:1, %), linoleic acid (C18:2), and linolenic acid (C18:3).

[0035] In order to further illustrate the present invention, the following describes the solutions provided by the present invention in detail in conjunction with embodiments, but they cannot be understood as limiting the protection scope of the present invention.

[0036] Example 1

[0037] A preparation method of a coconut meal feed, the steps are as follows:

[0038] (1) Prepare the composite lactic acid bacteria stock:

[0039] The composite lactic acid bacteria stock is composed of Lactobacillus plantarum, Lactobacillus acidophilus, Bifidobacterium adolescentis, and Saccharomyces cerevisiae according to a mass ratio of 1:0.3:0.6:0.03; the Lactobacillus plantarum, Lactobacillus acidophilus, Bifidobacterium adolescentis, and Saccharomyces cerevisiae are all obtained by regular purchase;

[0040] The effective viable counts of Lactobacillus plantarum, Lactobacillus acidophilus, Bifidobacterium adolescentis, and Saccharomyces cerevisiae are all 1×10 8 cfu / g.

[0041] (2) Prepare the culture solution:

[0042] By mass, the composition includes the following raw materials: 15 parts of glucose, 10 parts of calcium carbonate, 0.28 parts of magnesium sulfate, 1 part of manganese sulfate, and 0.05 parts of dipotassium hydrogen phosphate;

[0043] Put 100 jin (i.e., 50 L) of well water in a plastic bucket, heat the well water to 35°C, add 2 kg of brown sugar and melt it, then add 0.5 kg of milk powder, 0.5 kg of the composition, and 0.2 kg of the original compound lactic acid bacteria. Stir the above substances evenly, seal them with inner and outer lids, and expand and propagate at 33°C for 48 h to obtain a compound lactic acid bacteria expanded culture solution with an effective viable count > 5×10 8 cfu / mL.

[0044] (3) Prepare coconut flake feed

[0045] Convey 1000 kg of coconut flakes to a blender by a conveyor belt. At the same time, draw 25 kg of the compound lactic acid bacteria expanded culture solution into the blender with a sprayer, and spray and stir evenly with the coconut flakes for 10 min, and anaerobically ferment at 30°C for 7 d to obtain coconut flake feed.

[0046] Example 2

[0047] A preparation method of coconut flake feed, the steps are as follows:

[0048] (1) Prepare the original compound lactic acid bacteria:

[0049] The original compound lactic acid bacteria is composed of Lactobacillus plantarum, Lactobacillus acidophilus, Bifidobacterium adolescentis, and Saccharomyces cerevisiae according to a mass ratio of 1:0.2:0.5:0.02;

[0050] The effective viable counts of Lactobacillus plantarum, Lactobacillus acidophilus, Bifidobacterium adolescentis, and Saccharomyces cerevisiae are all 1×10 8 cfu / g.

[0051] (2) Prepare the culture solution:

[0052] Calculated by mass parts, the composition includes the following raw materials: 15 parts of glucose, 10 parts of calcium carbonate, 0.28 parts of magnesium sulfate, 1 part of manganese sulfate, and 0.05 parts of dipotassium hydrogen phosphate;

[0053] Put 100 jin (i.e., 50 L) of well water in a plastic bucket, heat the well water to 35°, add 2 kg of brown sugar and melt it, then add 0.5 kg of milk powder, 0.5 kg of the composition, and 0.2 kg of the original compound lactic acid bacteria. Stir the above substances evenly, seal them with inner and outer lids, and expand and propagate at 33°C for 48 h to obtain a compound lactic acid bacteria expanded culture solution with an effective viable count > 5×10 8 cfu / mL.

[0054] (3) Prepare coconut flake feed

[0055] Convey 1000 kg of coconut flakes to a blender by a conveyor belt. At the same time, draw 25 kg of the compound lactic acid bacteria expanded culture solution into the blender with a sprayer, and spray and stir evenly with the coconut flakes for 10 min, and anaerobically ferment at 30°C for 7 d to obtain coconut flake feed.

[0056] Example 3

[0057] A preparation method of coconut flake feed, the steps are as follows:

[0058] (1) Prepare the composite lactic acid bacteria stock culture:

[0059] The composite lactic acid bacteria stock culture is composed of Lactobacillus plantarum, Lactobacillus acidophilus, Bifidobacterium adolescentis and Saccharomyces cerevisiae according to a mass ratio of 1:0.3:0.6:0.03;

[0060] The effective viable counts of Lactobacillus plantarum, Lactobacillus acidophilus, Bifidobacterium adolescentis and Saccharomyces cerevisiae are all 1×10 8 cfu / g.

[0061] (2) Prepare the culture solution:

[0062] By mass, the composition includes the following raw materials: 20 parts of glucose, 10 parts of calcium carbonate, 0.25 part of magnesium sulfate, 1.2 parts of manganese sulfate and 0.05 part of dipotassium hydrogen phosphate;

[0063] Put 100 catties (i.e., 50L) of well water in a plastic bucket, heat the well water to 35°, add 2 kg of brown sugar and melt it, then add 0.5 kg of milk powder, 0.5 kg of the composition, and 0.2 kg of the composite lactic acid bacteria stock culture. Stir the above substances evenly, seal them with inner and outer lids, and expand and multiply at 33 degrees for 48 h to obtain a composite lactic acid bacteria expanded culture solution with an effective viable count > 5×10 8 cfu / mL.

[0064] (3) Prepare the coconut flake feed

[0065] Convey 1000 kg of coconut flakes to a mixer by a conveyor belt. At the same time, draw 25 kg of the composite lactic acid bacteria expanded culture solution into the mixer with a sprayer, and spray and stir evenly with the coconut flakes for 10 min. Anaerobic fermentation is carried out at 30°C for 7 d to obtain the coconut flake feed.

[0066] Comparative Example 1

[0067] A preparation method of coconut flake feed, the steps are as follows:

[0068] (1) Prepare the composite lactic acid bacteria stock culture:

[0069] The composite lactic acid bacteria stock culture is composed of Lactobacillus plantarum, Lactobacillus acidophilus, Bacillus subtilis and Saccharomyces cerevisiae according to a mass ratio of 1:0.3:0.6:0.03;

[0070] The effective viable counts of Lactobacillus plantarum, Lactobacillus acidophilus, Bacillus subtilis and Saccharomyces cerevisiae are all 1×10 8 cfu / g.

[0071] (2) Preparation of culture medium:

[0072] By mass fraction, the composition comprises the following raw materials: 15 parts of glucose, 10 parts of calcium carbonate, 0.28 part of magnesium sulfate, 1 part of manganese sulfate, and 0.05 part of dipotassium hydrogen phosphate;

[0073] Put 100 catties (i.e., 50L) of well water in a plastic bucket, heat the well water to 35°, add 2 kg of brown sugar and melt it, then add 0.5 kg of milk powder, 0.5 kg of the composition, and 0.2 kg of the original compound lactic acid bacteria. Stir the above substances evenly, seal them with inner and outer lids, and propagate at 33° for 48 h to obtain a compound lactic acid bacteria expanded culture solution with an effective viable count > 5×10 8 cfu / mL.

[0074] (3) Preparation of coconut meal feed

[0075] Convey 1000 kg of coconut meal to a blender by a conveyor belt. At the same time, draw 25 kg of the compound lactic acid bacteria expanded culture solution into the blender with a sprayer, and spray and stir it evenly with the coconut meal for 10 min. Ferment anaerobically at 30°C for 7 d to obtain coconut meal feed.

[0076] Comparative Example 2

[0077] A preparation method of coconut meal feed, the steps are as follows:

[0078] (1) Preparation of the original compound lactic acid bacteria:

[0079] The original compound lactic acid bacteria is composed of Lactobacillus plantarum, Lactobacillus acidophilus, Bifidobacterium adolescentis, and Saccharomyces cerevisiae according to a mass ratio of 1:0.3:0.6:0.03;

[0080] The effective viable counts of Lactobacillus plantarum, Lactobacillus acidophilus, Bifidobacterium adolescentis, and Saccharomyces cerevisiae are all 1×10 8 cfu / g.

[0081] (2) Preparation of culture medium:

[0082] By mass fraction, the composition comprises the following raw materials: 15 parts of glucose, 10 parts of calcium carbonate, 0.28 part of magnesium sulfate, 0.5 part of manganese sulfate, and 0.05 part of dipotassium hydrogen phosphate;

[0083] Put 100 catties (i.e., 50L) of well water in a plastic bucket, heat the well water to 35°, add 2 kg of brown sugar and melt it, then add 0.5 kg of milk powder, 0.5 kg of the composition, and 0.2 kg of the original compound lactic acid bacteria. Stir the above substances evenly, seal them with inner and outer lids, and propagate at 33° for 48 h to obtain a compound lactic acid bacteria expanded culture solution with an effective viable count > 5×10 8 cfu / mL.

[0084] (3) Preparation of coconut flake feed

[0085] Convey 1000 kg of coconut flakes to a blender using a conveyor belt. At the same time, draw 25 kg of the compound lactic acid bacteria expanded culture solution into the blender with a sprayer, and spray and uniformly stir it with the coconut flakes for 10 minutes, and anaerobically ferment for 7 days at 30 °C to obtain coconut flake feed.

[0086] Comparative Example 3

[0087] A preparation method of coconut flake feed, the steps are as follows:

[0088] (1) Preparation of the original compound lactic acid bacteria:

[0089] The original compound lactic acid bacteria is composed of Lactobacillus plantarum, Lactobacillus acidophilus, Bifidobacterium adolescentis and Saccharomyces cerevisiae according to a mass ratio of 1:0.3:0.6:0.03;

[0090] The effective viable counts of Lactobacillus plantarum, Lactobacillus acidophilus, Bifidobacterium adolescentis and Saccharomyces cerevisiae are all 1×10 8 cfu / g.

[0091] (2) Preparation of the culture solution:

[0092] Calculated by mass parts, the composition includes the following raw materials: 15 parts of glucose, 10 parts of calcium carbonate, 0.28 parts of magnesium sulfate, 0.05 parts of manganese sulfate and 0.05 parts of dipotassium hydrogen phosphate;

[0093] Put 100 catties (i.e., 50 L) of well water in a plastic bucket, heat the well water to 35 °C, add 2 kg of brown sugar and melt it, then add 0.5 kg of milk powder, 0.5 kg of the composition, and 0.2 kg of the original compound lactic acid bacteria. Stir the above substances evenly, seal them with inner and outer lids, and expand and propagate at 33 °C for 48 h to obtain a compound lactic acid bacteria expanded culture solution with an effective viable count > 5×10 8 cfu / mL.

[0094] (3) Preparation of coconut flake feed

[0095] Convey 1000 kg of coconut flakes to a blender using a conveyor belt. At the same time, draw 25 kg of the compound lactic acid bacteria expanded culture solution into the blender with a sprayer, and spray and uniformly stir it with the coconut flakes for 10 minutes, and anaerobically ferment for 7 days at 30 °C to obtain coconut flake feed.

[0096] Comparative Example 4

[0097] A preparation method of coconut flake feed, the steps are as follows:

[0098] (1) Preparation of the original compound lactic acid bacteria:

[0099] The original compound lactic acid bacteria are composed of Lactobacillus plantarum, Lactobacillus acidophilus, Bifidobacterium adolescentis, and Saccharomyces cerevisiae in a mass ratio of 1:0.3:0.6:0.03;

[0100] The effective viable counts of Lactobacillus plantarum, Lactobacillus acidophilus, Bifidobacterium adolescentis, and Saccharomyces cerevisiae are all 1×10 8 cfu / g.

[0101] (2) Preparation of the culture medium:

[0102] By mass, the composition includes the following raw materials: 15 parts of glucose, 10 parts of calcium carbonate, 0.28 parts of magnesium sulfate, 1 part of manganese sulfate, and 0.05 parts of dipotassium hydrogen phosphate;

[0103] Put 100 catties (i.e., 50L) of well water in a plastic bucket, heat the well water to 35°, add 2 kg of brown sugar and melt it, then add 0.5 kg of milk powder, 0.5 kg of the composition, and 0.2 kg of the original compound lactic acid bacteria. Stir the above substances evenly, seal them with inner and outer lids, and expand and multiply at 33 degrees for 48 hours to obtain a compound lactic acid bacteria expanded culture solution with an effective viable count > 5×10 8 cfu / mL.

[0104] (3) Preparation of the coconut meal feed

[0105] Use a conveyor belt to transport 1000 kg of the coconut meal mixture (the coconut meal mixture is composed of coconut meal and soybean meal in a mass ratio of 5:1) to a mixer. At the same time, draw 25 kg of the compound lactic acid bacteria expanded culture solution into the mixer with a sprayer, and spray and stir it evenly with the coconut meal for 10 minutes. Anaerobic fermentation is carried out at 30°C for 7 days to obtain the coconut meal feed.

[0106] Application Example 1

[0107] Isolate the same pigsty into several pens with the same layout and relatively independent by plastic sheets to control the same feeding environment in the pigsty; select 80 fattening pigs with basically the same initial weight and good health, and randomly divide them into 8 groups, with 10 pigs in each group.

[0108] The fattening feed is composed of corn: soybean cake: fine bran: flour: special premix for fattening pigs in a mass ratio of 60:20:18:6:3;

[0109] Treatment 1: Mix the fattening feed with the coconut meal feed in Example 1 in a mass ratio of 6.5:3.5, that is, the addition amount of the coconut meal feed is 35 wt% of the total amount of the basal diet;

[0110] Treatment 2: Mix the fattening feed with the coconut meal feed in Example 2 in a mass ratio of 6.5:3.5;

[0111] Treatment 3: It is prepared by mixing the fattening feed and the coconut flake feed in Example 3 at a mass ratio of 6.5:3.5;

[0112] Treatment 4: It is prepared by mixing the fattening feed and the coconut flake feed in Comparative Example 1 at a mass ratio of 6.5:3.5;

[0113] Treatment 5: It is prepared by mixing the fattening feed and the coconut flake feed in Comparative Example 2 at a mass ratio of 6.5:3.5;

[0114] Treatment 6: It is prepared by mixing the fattening feed and the coconut flake feed in Comparative Example 3 at a mass ratio of 6.5:3.5;

[0115] Treatment 7: It is prepared by mixing the fattening feed and the coconut flake feed in Comparative Example 4 at a mass ratio of 6.5:3.5;

[0116] Blank control: Fattening feed.

[0117] According to the feeds in Treatments 1 - 7 and the blank control, fattening pigs are fed. The feed is put in three times a day (the feeding amount in each treatment is the same at each feeding time), allowing the fattening pigs to eat freely and drink water freely, and the feces are cleaned every two days; The experimental period is 28 days. Starting from the 19th day of the experiment, the ammonia concentration in the pig house is measured at 12:00 noon every day. After continuously measuring for 10 days, the average value of ammonia is calculated, and the results are shown in Table 1.

[0118] Table 1 Effects of different treatments on the feeding of fattening pigs

[0119] Treatment Initial average weight (kg) Final average weight (kg) Average ammonia emissions (ppm) Treatment 1 62.9 93.5 2.4 Treatment 2 62.8 92.8 2.3 Treatment 3 63.2 94.1 2.5 Treatment 4 63.1 88.1 8.5 Treatment 5 62.7 90.5 4.3 Treatment 6 63.2 89.2 7.8 Treatment 7 62.9 88.7 8.7 Blank control 63.0 87.8 13.1

[0120] Combined with the data in Table 1, it can be seen that the coconut flake feeds prepared in Examples 1 - 3 are added in Treatments 1 - 3 respectively. It not only helps the fattening pigs gain weight, but also reduces the ammonia emissions. The average ammonia emissions in the pig house are only 2.3 - 2.5 ppm, which is beneficial to reducing the odor in the pig house; while after the coconut flake feeds prepared in Comparative Examples 3 - 4 are added in Treatments 4 - 7 respectively, the weight gain of the fattening pigs is less, and the ammonia emissions are high.

[0121] Application Example 2

[0122] According to the results in Application Example 1, the components of the lard (obtained by frying the fat in pork) in the fattening pigs raised in Treatments 1 - 3, Treatment 5 and the blank control are detected. It is carried out according to the third method in GB 5009.168 - 2016 "National Food Safety Standard - Determination of Fatty Acids in Foods", and the results are shown in Table 2.

[0123] Table 2 Contents of fatty acids in different treatments

[0124]

[0125] It can be seen from the data in Table 2 that after feeding the feeds in Feeding Treatments 1-3 (i.e., adding the coconut flake feeds prepared in Examples 1-3), it is beneficial to enrich the content and types of fatty acids in lard, improve the flavor of the meat quality. However, in Treatment 5 and the blank control, C14:0 myristic acid or C18:0 stearic acid, etc. are lacking.

[0126] In summary, in the present invention, a composite lactic acid bacteria stock containing Lactobacillus plantarum, Lactobacillus acidophilus, Bifidobacterium adolescentis, and Saccharomyces cerevisiae is expanded by using a high content of manganese sulfate and a low content of dipotassium hydrogen phosphate. This not only activates the enzymes in the microorganisms, can promote the metabolic reactions of the microorganisms, improve the fermentation efficiency, but also can affect the growth and metabolism of the microorganisms, change the composition and properties of the fermentation products; by subjecting coconut flakes to low-temperature freezing and room-temperature standing, the biological activity of the secondary metabolites in the coconut flakes is significantly improved. The sequentially prepared coconut flake feeds have good palatability and high nutritional value, can be used to improve the types and content of fatty acids in the meat quality flavor, can not only promote animal growth but also reduce ammonia emissions, and are green and environmentally friendly.

[0127] Although the above embodiments have made a detailed description of the present invention, they are only a part of the embodiments of the present invention, rather than all embodiments. People can also obtain other embodiments based on these embodiments without creative efforts, and these embodiments all fall within the protection scope of the present invention.

Claims

1. A method for preparing a desiccated coconut feed, characterized in that: The steps include: The composite lactic acid bacteria stock is inoculated into the culture solution for expansion and cultivation to obtain the composite lactic acid bacteria expansion culture solution; The composite lactic acid bacteria culture solution is mixed with shredded coconut and then subjected to anaerobic fermentation to obtain shredded coconut feed; The composite lactic acid bacteria stock comprises: Lactobacillus plantarum, Lactobacillus acidophilus, Bifidobacterium adolescentis and Saccharomyces cerevisiae; The culture solution includes: manganese sulfate and dipotassium hydrogen phosphate.

2. The preparation method according to claim 1, characterized in that: The culture solution uses water as a solvent and includes the following components: 40-50 g / L of brown sugar, 10-20 g / L of milk powder and 10-20 g / L of a combination; The composition comprises the following raw materials in parts by mass: 15-20 parts of glucose, 10-12 parts of calcium carbonate, 0.25-0.28 parts of magnesium sulfate, 1-1.2 parts of manganese sulfate and 0.03-0.05 parts of dipotassium hydrogen phosphate.

3. The preparation method according to claim 1 or 2, characterized in that: The composite lactic acid bacteria stock is composed of Lactobacillus plantarum, Lactobacillus acidophilus, Bifidobacterium adolescentis and Saccharomyces cerevisiae in a mass ratio of 1:0.2-0.3:0.4-0.6:0.02-0.03; The effective viable counts of Lactobacillus plantarum, Lactobacillus acidophilus, Bifidobacterium adolescentis and Saccharomyces cerevisiae are 1×10 8 -2×10 8 cfu / g.

4. The preparation method according to claim 1, characterized in that: The mass ratio of the composite lactic acid bacteria stock to the culture solution is 0.3-0.5:100; The conditions for the propagation culture include: a temperature of 33-35° C. and a time of 44-50 h.

5. The preparation method according to claim 1, characterized in that: During the anaerobic fermentation, the mass ratio of the composite lactic acid bacteria culture liquid to the coconut flakes is 2-2.5:100; The effective viable bacteria count of the compound lactic acid bacteria culture solution is >5×10 8 cfu / mL.

6. The preparation method according to claim 1, characterized in that: The conditions of the anaerobic fermentation include: a temperature of 30-36° C. and a time of 6-8 days.

7. The desiccated coconut feed prepared by the preparation method according to any one of claims 1 to 6.

8. The application of the desiccated coconut feed according to claim 7, comprising: One or more of promoting animal growth, improving meat flavor and reducing ammonia emissions.

9. The use according to claim 8, characterized in that: The animals include: one or more of chicken, duck, goose, pig, fish, sheep and cattle.

10. A method for improving the flavor of animal meat and / or promoting animal growth, characterized in that: The steps include: Feeding the coconut feed according to claim 7 to animals; The added amount of the coconut feed is 15wt%-60wt% of the total amount of the basic diet.

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